FvH4_5g35740

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004574
Physical Location & Seq
Forward (+)
26176726 .. 26179882
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35740.t1

Sequence Viewer

Length: 762 bp
ATGGCGTCCTCCGTCATCCCCGATAACCTGACCCGAGAGCAGTACGTCTACCTCGCCAAGCTCGCCGAGCAGGCCGAGCGCTACGAGGAGATGGTCCAGTTCATGGAGAAGCTTGTGGTGGGGTGCACCCCGGCGTCGGAGCTGACGGTGGAGGAGCGCAACCTCCTCTCCGTCGCCTACAAGAACGTGATCGGGTCGCTCCGGGCGGCGTGGCGGATCGTGTCGTCGATCGAGCAGAAGGAGGAGGGGCGCAAGAACGAGGACCACGTGGCGCTCGTCAAGGAGTACAGATCCAAGGTCGAGGCGGAGCTCTCAGACGTCTGCGCCAGCATCTTGAAGATCTTGGACTCCAACCTGGTCCCGTCGGCGTCGTCGAGCGAGTCAAAGGTGTTCTATTTGAAGATGAAGGGGGACTACCACCGCTACTTGGCGGAGTTCAAGTCGGCTGAGGAGAGGAAGGCCGCTGCTGAGGACACTATGCTCTCCTACAAGGCCGCTCAGGATGTGGCTATGACGGATCTGGCGCCAACTCACCCCATAAGATTGGGTCTGGCTCTGAACTTCTCAGTGTTCTACTATGAGATTCTCAATCAGTCTGATAAGGCTTGTAGCATGGCCAAACAGGCATTTGAGGAAGCTATTGCTGAGCTAGACACTTTGGGAGAAGAATCTTACAAGGACAGCACTCTGATCATGCAACTTTTGAGGGACAACCTCACCCTTTGGACCTCAGATGTGACGGAAGGGTTAGATGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

254

Amino Acids

28.49

Weight (kDa)

4.74

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 18 - 243 6.3e-100 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 523
AccB7I CCANNNNNTGG 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 1 cut(s) 48
AciI CCGC 7 cut(s) 206, 214, 305, 421, 431, 462, 495
AclWI GGATC 3 cut(s) 224, 285, 525
AcoI YGGCCR 1 cut(s) 615
AcvI CACGTG 1 cut(s) 268
AcyI GRCGYC 5 cut(s) 5, 134, 318, 368, 524
AfaI GTAC 2 cut(s) 44, 287
AfeI AGCGCT 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 103, 136, 427
AgsI TTSAA 3 cut(s) 337, 400, 439
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 61, 112, 142, 310, 638, 649
AluI AGCT 6 cut(s) 61, 112, 142, 310, 638, 649
Alw21I GWGCWC 2 cut(s) 128, 312
Alw44I GTGCAC 1 cut(s) 124
AlwI GGATC 3 cut(s) 224, 285, 525
Ama87I CYCGRG 1 cut(s) 33
Aor51HI AGCGCT 1 cut(s) 80
AoxI GGCC 4 cut(s) 72, 459, 492, 615
ApaLI GTGCAC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 464
AspLEI GCGC 6 cut(s) 81, 159, 252, 274, 326, 526
AspS9I GGNCC 4 cut(s) 94, 262, 358, 726
AsuC2I CCSGG 2 cut(s) 131, 203
AsuHPI GGTGA 2 cut(s) 524, 709
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 4 cut(s) 94, 262, 358, 726
BaeGI GKGCMC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbrPI CACGTG 1 cut(s) 268
Bbv12I GWGCWC 2 cut(s) 128, 312
BbvCI CCTCAGC 2 cut(s) 447, 468
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 356
BclI TGATCA 1 cut(s) 690
BcnI CCSGG 2 cut(s) 131, 203
BfaI CTAG 1 cut(s) 650
BfoI RGCGCY 3 cut(s) 82, 275, 527
BglI GCCNNNNNGGC 2 cut(s) 71, 623
BglII AGATCT 1 cut(s) 339
BisI GCNGC 4 cut(s) 207, 462, 465, 495
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 4 cut(s) 208, 463, 466, 496
Bme1390I CCNGG 3 cut(s) 131, 203, 356
Bme18I GGWCC 4 cut(s) 94, 262, 358, 726
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 4 cut(s) 94, 262, 358, 726
BmiI GGNNCC 2 cut(s) 360, 525
BmrFI CCNGG 3 cut(s) 131, 203, 356
BmsI GCATC 1 cut(s) 339
Bpu10I CCTNAGC 3 cut(s) 447, 468, 498
Bpu1102I GCTNAGC 1 cut(s) 645
BpuMI CCSGG 2 cut(s) 131, 203
BsaAI YACGTR 1 cut(s) 268
BsaHI GRCGYC 5 cut(s) 5, 134, 318, 368, 524
BsaJI CCNNGG 2 cut(s) 129, 294
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 136, 427
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 129, 294
BseGI GGATG 2 cut(s) 15, 508
BseLI CCNNNNNNNGG 3 cut(s) 103, 136, 427
BseMII CTCAG 7 cut(s) 327, 438, 459, 512, 579, 636, 744
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 5 cut(s) 101, 155, 167, 257, 464
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 74, 461, 494, 617
BshNI GGYRCC 1 cut(s) 523
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 2 cut(s) 128, 312
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 2 cut(s) 131, 202
BslFI GGGAC 3 cut(s) 344, 425, 722
BslI CCNNNNNNNGG 3 cut(s) 103, 136, 427
BsmFI GGGAC 3 cut(s) 344, 425, 722
BsnI GGCC 4 cut(s) 74, 461, 494, 617
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 128, 312
Bsp143I GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
Bsp1720I GCTNAGC 1 cut(s) 645
BspACI CCGC 7 cut(s) 206, 214, 305, 421, 431, 462, 495
BspANI GGCC 4 cut(s) 74, 461, 494, 617
BspCNI CTCAG 7 cut(s) 326, 439, 460, 511, 578, 637, 743
BspLI GGNNCC 2 cut(s) 360, 525
BspPI GGATC 3 cut(s) 224, 285, 525
BspT107I GGYRCC 1 cut(s) 523
BsrBI CCGCTC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 129, 294
BssMI GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
BssNI GRCGYC 5 cut(s) 5, 134, 318, 368, 524
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 148
BstACI GRCGYC 5 cut(s) 5, 134, 318, 368, 524
BstBAI YACGTR 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 63, 72, 328
BstDEI CTNAG 7 cut(s) 313, 447, 468, 498, 565, 645, 730
BstF5I GGATG 2 cut(s) 15, 508
BstH2I RGCGCY 3 cut(s) 82, 275, 527
BstHHI GCGC 6 cut(s) 81, 159, 252, 274, 326, 526
BstKTI GATC 7 cut(s) 192, 219, 231, 293, 342, 520, 693
BstMBI GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 62, 67, 71, 76, 623
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 129, 201, 354
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 451
BstX2I RGATCY 3 cut(s) 290, 339, 517
BstXI CCANNNNNNTGG 1 cut(s) 544
BstYI RGATCY 3 cut(s) 290, 339, 517
BsuRI GGCC 4 cut(s) 74, 461, 494, 617
BtsCI GGATG 2 cut(s) 15, 508
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 3 cut(s) 63, 72, 328
CfoI GCGC 6 cut(s) 81, 159, 252, 274, 326, 526
Cfr13I GGNCC 4 cut(s) 94, 262, 358, 726
CseI GACGC 2 cut(s) 123, 357
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 2 cut(s) 43, 286
CviAII CATG 3 cut(s) 103, 613, 694
CviQI GTAC 2 cut(s) 43, 286
DdeI CTNAG 7 cut(s) 313, 447, 468, 498, 565, 645, 730
DinI GGCGCC 1 cut(s) 525
DpnI GATC 7 cut(s) 191, 218, 230, 292, 341, 519, 692
DpnII GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
EaeI YGGCCR 1 cut(s) 615
EciI GGCGGA 3 cut(s) 229, 320, 446
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 4 cut(s) 94, 262, 358, 726
Eco47III AGCGCT 1 cut(s) 80
Eco53kI GAGCTC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 268
Eco88I CYCGRG 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 106, 616, 697
FaiI YATR 8 cut(s) 104, 479, 512, 539, 579, 614, 695, 760
FaqI GGGAC 3 cut(s) 344, 425, 722
FatI CATG 3 cut(s) 102, 612, 693
FbaI TGATCA 1 cut(s) 690
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 207, 462, 465, 495
FokI GGATG 2 cut(s) 2, 515
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 4 cut(s) 207, 462, 465, 495
FspBI CTAG 1 cut(s) 650
GlaI GCGC 6 cut(s) 80, 158, 251, 273, 325, 525
GluI GCNGC 4 cut(s) 207, 462, 465, 495
HaeII RGCGCY 3 cut(s) 82, 275, 527
HaeIII GGCC 4 cut(s) 74, 461, 494, 617
HapII CCGG 2 cut(s) 131, 202
HgaI GACGC 2 cut(s) 123, 357
HhaI GCGC 6 cut(s) 81, 159, 252, 274, 326, 526
Hin1I GRCGYC 5 cut(s) 5, 134, 318, 368, 524
Hin1II CATG 3 cut(s) 106, 616, 697
Hin6I GCGC 6 cut(s) 79, 157, 250, 272, 324, 524
HinP1I GCGC 6 cut(s) 79, 157, 250, 272, 324, 524
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 4 cut(s) 347, 380, 583, 668
HpaII CCGG 2 cut(s) 131, 202
HphI GGTGA 2 cut(s) 524, 709
Hpy166II GTNNAC 2 cut(s) 49, 126
Hpy188I TCNGA 6 cut(s) 139, 316, 558, 598, 690, 733
Hpy188III TCNNGA 2 cut(s) 334, 500
Hpy8I GTNNAC 2 cut(s) 49, 126
Hpy99I CGWCG 6 cut(s) 139, 176, 229, 367, 373, 376
HpyAV CCTTC 4 cut(s) 232, 400, 451, 737
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 4 cut(s) 45, 186, 267, 318
HpyCH4V TGCA 2 cut(s) 126, 697
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 67, 71, 76, 623
HpyF3I CTNAG 7 cut(s) 313, 447, 468, 498, 565, 645, 730
HpySE526I ACGT 4 cut(s) 45, 186, 267, 318
Hsp92I GRCGYC 5 cut(s) 5, 134, 318, 368, 524
Hsp92II CATG 3 cut(s) 106, 616, 697
HspAI GCGC 6 cut(s) 79, 157, 250, 272, 324, 524
KasI GGCGCC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 690
Kzo9I GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
LmnI GCTCC 4 cut(s) 139, 154, 204, 307
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 339
MabI ACCWGGT 1 cut(s) 354
MaeI CTAG 1 cut(s) 650
MaeII ACGT 4 cut(s) 45, 186, 267, 318
MaeIII GTNAC 1 cut(s) 736
MalI GATC 7 cut(s) 191, 218, 230, 292, 341, 519, 692
MbiI CCGCTC 1 cut(s) 497
MboI GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
MboII GAAGA 3 cut(s) 349, 412, 677
MflI RGATCY 3 cut(s) 290, 339, 517
MhlI GDGCHC 2 cut(s) 128, 312
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 2 cut(s) 341, 389
MmeI TCCRAC 2 cut(s) 117, 375
Mox20I TGGCCA 1 cut(s) 617
MscI TGGCCA 1 cut(s) 617
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 131, 202
MspR9I CCNGG 3 cut(s) 131, 203, 356
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 5 cut(s) 62, 67, 71, 76, 623
NarI GGCGCC 1 cut(s) 524
NciI CCSGG 2 cut(s) 131, 203
NdeII GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
NlaIII CATG 3 cut(s) 106, 616, 697
NlaIV GGNNCC 2 cut(s) 360, 525
NmeAIII GCCGAG 2 cut(s) 91, 100
NmuCI GTSAC 1 cut(s) 736
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 273, 371
PfeI GAWTC 2 cut(s) 583, 668
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 4 cut(s) 208, 463, 466, 496
Ple19I CGATCG 1 cut(s) 231
PleI GAGTC 2 cut(s) 341, 388
PluTI GGCGCC 1 cut(s) 527
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
PpsI GAGTC 2 cut(s) 341, 388
Ppu21I YACGTR 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 354
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 360, 525
PspPI GGNCC 4 cut(s) 94, 262, 358, 726
PsuI RGATCY 3 cut(s) 290, 339, 517
PvuI CGATCG 1 cut(s) 231
RsaI GTAC 2 cut(s) 44, 287
RsaNI GTAC 2 cut(s) 43, 286
SacI GAGCTC 1 cut(s) 312
SatI GCNGC 4 cut(s) 207, 462, 465, 495
Sau3AI GATC 7 cut(s) 189, 216, 228, 290, 339, 517, 690
Sau96I GGNCC 4 cut(s) 94, 262, 358, 726
SchI GAGTC 2 cut(s) 341, 389
ScrFI CCNGG 3 cut(s) 131, 203, 356
SduI GDGCHC 2 cut(s) 128, 312
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 1 cut(s) 339
SfoI GGCGCC 1 cut(s) 525
SinI GGWCC 4 cut(s) 94, 262, 358, 726
SsiI CCGC 7 cut(s) 206, 214, 305, 421, 431, 462, 495
SspDI GGCGCC 1 cut(s) 523
SspMI CTAG 1 cut(s) 650
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 129, 201, 354
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 4 cut(s) 48, 189, 270, 321
TaqI TCGA 4 cut(s) 227, 231, 300, 374
TatI WGTACW 1 cut(s) 285
TauI GCSGC 3 cut(s) 209, 464, 497
TfiI GAWTC 2 cut(s) 583, 668
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 2 cut(s) 91, 419
TspGWI ACGGA 3 cut(s) 160, 530, 755
TspRI CASTG 1 cut(s) 573
Van91I CCANNNNNTGG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 124
VpaK11BI GGWCC 4 cut(s) 94, 262, 358, 726
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 650
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.