Rorug07G0302900

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002637
Physical Location & Seq
Forward (+)
30133116 .. 30137180
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0302900.1

Sequence Viewer

Length: 957 bp
ATGATGAGCTCTTCTGAAGAGTCAGGAAAGAATGCCAAGTCCGTGCATCATGTGAGAGAAGACAGTGAATATGTTAGGCTGGTTATATCCAACGAAACAAACACAGCAGAAGCAGATGTCTTGCAGCCTCAATCAGAAGCAGGGGTTAAATCTTTTTGGTGGTGGATTAGGGCCTTAGCCTTGTGCCTTGTCACCATTGTAGTGCTTCTCATTTTCTTAAAATGGGGGGTTCCGTTTCTTTTTGAAAAGGTTCTGTTGCCAATAATGCGATGGGAAGCCACAGCCTTTGGCCGTCCAGTTCTTGCCATTGTTCTCGTTGCTTCTCTGGCATTGTTCCCTGTGGTCTTGATCCCTTCTGGCCCTTCCATGTGGTTGGCTGGAATGATTTTTGGTTATGGCTTCGGATTTGTTATAATCATGGTTGGAACAACTATTGGGATGATCCTACCATATTTGATTGGTCTGTATTTCCGCGACCGCATCCATCAATGGTTAAAGAGATGGCCTCGAAACGCTGCAATGATTAGACTTGCTGGGGAAGGGAGTTGGCTCCATCAATTTCGAGTGGTTGCTCTCTTTAGGGTTTCACCCTTTCCATACACAATTTTCAATTACGCAATCGTGGTTACAAGTATGACATTTTGGCCCTACTTGTGGGGATCAATTGCTGGGATGGTGCCAGAAGCTTTTATTTACATCTACAGTGGTCGCTTAATAAGGACATTTGCGGATGTCAAGTATGGGAACTATCACATGACTACCGTGGAGATTGTATACAACACTCTATCCTTAATTGTTGCAATCGTAACCACAGTTGCTTTTACTGTTTATGCCAAAAATGCTTTGAATGAGCTGAAGAGGGCAGAGACTAGTGGTGTGGAAGCATCTGCTTCTGACCATATCAGCTTACAGATGGAGAAGCTTCCACTTGAAAAACCGCATATTTTTACGTTGTAG

Protein Analysis

318

Amino Acids

35.97

Weight (kDa)

9.06

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 117 - 237 3.7e-20 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 774
AccII CGCG 1 cut(s) 474
AciI CCGC 4 cut(s) 472, 478, 728, 938
AclWI GGATC 3 cut(s) 343, 436, 667
AcoI YGGCCR 1 cut(s) 289
AcuI CTGAAG 2 cut(s) 36, 875
AfiI CCNNNNNNNGG 1 cut(s) 654
AgsI TTSAA 4 cut(s) 245, 610, 847, 932
AhlI ACTAGT 1 cut(s) 869
AluBI AGCT 5 cut(s) 9, 686, 853, 906, 922
AluI AGCT 5 cut(s) 9, 686, 853, 906, 922
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 3 cut(s) 343, 436, 667
AoxI GGCC 5 cut(s) 171, 289, 358, 503, 644
ApeKI GCWGC 2 cut(s) 124, 515
Asp700I GAANNNNTTC 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 171, 359, 645
AsuHPI GGTGA 2 cut(s) 184, 579
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 136, 502
BccI CCATC 6 cut(s) 264, 492, 495, 561, 667, 907
BceAI ACGGC 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 860
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 1 cut(s) 870
BfmI CTRYAG 1 cut(s) 700
BisI GCNGC 2 cut(s) 125, 516
BlsI GCNGC 2 cut(s) 126, 517
BmgT120I GGNCC 3 cut(s) 171, 359, 645
BmiI GGNNCC 3 cut(s) 231, 551, 678
BmsI GCATC 3 cut(s) 55, 489, 893
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 490, 522
Bpu10I CCTNAGC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 762
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 4 cut(s) 444, 480, 678, 736
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMI GCAATG 1 cut(s) 525
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 136, 502
BseYI CCCAGC 2 cut(s) 533, 668
Bsh1236I CGCG 1 cut(s) 474
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 5 cut(s) 173, 291, 360, 505, 646
BshNI GGYRCC 1 cut(s) 676
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 1 cut(s) 860
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 5 cut(s) 173, 291, 360, 505, 646
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 348, 441, 659
BspACI CCGC 4 cut(s) 472, 478, 728, 938
BspANI GGCC 5 cut(s) 173, 291, 360, 505, 646
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 231, 551, 678
BspPI GGATC 3 cut(s) 343, 436, 667
BspQI GCTCTTC 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 676
BsrDI GCAATG 1 cut(s) 525
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 3 cut(s) 348, 441, 659
BssNAI GTATAC 1 cut(s) 775
Bst1107I GTATAC 1 cut(s) 775
Bst4CI ACNGT 5 cut(s) 65, 704, 763, 814, 826
Bst6I CTCTTC 3 cut(s) 12, 16, 851
BstDEI CTNAG 1 cut(s) 175
BstDSI CCRYGG 1 cut(s) 762
BstF5I GGATG 4 cut(s) 444, 480, 678, 736
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 3 cut(s) 351, 444, 662
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 3 cut(s) 348, 441, 659
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 3 cut(s) 265, 326, 839
BstSFI CTRYAG 1 cut(s) 700
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 2 cut(s) 136, 502
BstV2I GAAGAC 1 cut(s) 66
BstXI CCANNNNNNTGG 1 cut(s) 373
BstZ17I GTATAC 1 cut(s) 775
BsuRI GGCC 5 cut(s) 173, 291, 360, 505, 646
BtgI CCRYGG 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 4 cut(s) 444, 480, 678, 736
BtsIMutI CAGTG 2 cut(s) 70, 709
Cfr13I GGNCC 3 cut(s) 171, 359, 645
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 4 cut(s) 50, 367, 418, 754
DdeI CTNAG 1 cut(s) 175
DpnI GATC 3 cut(s) 350, 443, 661
DpnII GATC 3 cut(s) 348, 441, 659
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 3 cut(s) 12, 16, 851
EarI CTCTTC 3 cut(s) 12, 16, 851
Ecl136II GAGCTC 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 11
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 36, 875
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 4 cut(s) 53, 370, 421, 757
FatI CATG 4 cut(s) 49, 366, 417, 753
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 125, 516
FokI GGATG 4 cut(s) 451, 467, 685, 743
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 2 cut(s) 125, 516
FspBI CTAG 1 cut(s) 870
GluI GCNGC 2 cut(s) 125, 516
GsaI CCCAGC 2 cut(s) 537, 672
HaeIII GGCC 5 cut(s) 173, 291, 360, 505, 646
Hin1II CATG 4 cut(s) 53, 370, 421, 757
HindIII AAGCTT 2 cut(s) 684, 920
HinfI GANTC 1 cut(s) 20
HphI GGTGA 2 cut(s) 184, 579
Hpy166II GTNNAC 1 cut(s) 775
Hpy188I TCNGA 4 cut(s) 16, 136, 404, 895
Hpy188III TCNNGA 2 cut(s) 24, 346
Hpy8I GTNNAC 1 cut(s) 775
HpyAV CCTTC 3 cut(s) 363, 372, 533
HpyCH4III ACNGT 5 cut(s) 65, 704, 763, 814, 826
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 4 cut(s) 46, 124, 518, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 326, 839
HpyF3I CTNAG 1 cut(s) 175
HpySE526I ACGT 1 cut(s) 950
Hsp92II CATG 4 cut(s) 53, 370, 421, 757
Kzo9I GATC 3 cut(s) 348, 441, 659
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 1 cut(s) 555
Lsp1109I GCAGC 2 cut(s) 136, 502
LweI GCATC 3 cut(s) 55, 489, 893
MaeI CTAG 1 cut(s) 870
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 3 cut(s) 190, 625, 805
MalI GATC 3 cut(s) 350, 443, 661
MboI GATC 3 cut(s) 348, 441, 659
MboII GAAGA 4 cut(s) 3, 29, 71, 868
MfeI CAATTG 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 5 cut(s) 557, 603, 610, 663, 792
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 2 cut(s) 114, 403
MnlI CCTC 3 cut(s) 138, 516, 852
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 5 cut(s) 147, 218, 494, 713, 791
MslI CAYNNNNRTG 1 cut(s) 200
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 37
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 3 cut(s) 265, 326, 839
NdeII GATC 3 cut(s) 348, 441, 659
NlaIII CATG 4 cut(s) 53, 370, 421, 757
NlaIV GGNNCC 3 cut(s) 231, 551, 678
NmuCI GTSAC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 16
PctI GAATGC 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 249
PkrI GCNGC 2 cut(s) 126, 517
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PsiI TTATAA 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 533, 668
PspN4I GGNNCC 3 cut(s) 231, 551, 678
PspPI GGNCC 3 cut(s) 171, 359, 645
RseI CAYNNNNRTG 1 cut(s) 200
SacI GAGCTC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 5 cut(s) 147, 218, 494, 713, 791
SatI GCNGC 2 cut(s) 125, 516
Sau3AI GATC 3 cut(s) 348, 441, 659
Sau96I GGNCC 3 cut(s) 171, 359, 645
SchI GAGTC 1 cut(s) 29
SduI GDGCHC 1 cut(s) 11
SetI ASST 7 cut(s) 11, 252, 688, 855, 908, 924, 953
SfaNI GCATC 3 cut(s) 55, 489, 893
SfcI CTRYAG 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 200
SpeI ACTAGT 1 cut(s) 869
Sse9I AATT 5 cut(s) 557, 603, 610, 663, 792
SsiI CCGC 4 cut(s) 472, 478, 728, 938
SspMI CTAG 1 cut(s) 870
SstI GAGCTC 1 cut(s) 11
TaaI ACNGT 5 cut(s) 65, 704, 763, 814, 826
TaiI ACGT 1 cut(s) 953
TaqI TCGA 2 cut(s) 508, 562
TasI AATT 5 cut(s) 557, 603, 610, 663, 792
Tru1I TTAA 5 cut(s) 147, 218, 494, 713, 791
Tru9I TTAA 5 cut(s) 147, 218, 494, 713, 791
TscAI CASTG 2 cut(s) 70, 709
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 124, 515
Tsp45I GTSAC 1 cut(s) 190
TspGWI ACGGA 2 cut(s) 31, 222
TspRI CASTG 2 cut(s) 70, 709
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.