AT3G54860

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002637
Physical Location & Seq
Reverse (-)
20324088 .. 20330030
5943 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54860.2

Sequence Viewer

Length: 1779 bp
ATGGCGCAGATCCCCAGCTTGGAGAATGCTCCTCTCAATCTGAAATCCATTAGGGACAAGTCTGAGAGGGAGCTCGTAAACTTGCTTAAAGACGTTCGTGGAACTAAGTGTTTGGTTATTGATCCAAAGCTCAGTGGTTCAGTCTCATTGATCATCCCGACCTCTAAGCTTAAGGAACTTGGTTTGGAGTTACGACATCTTACTGCGGAACCAGTTCAGACTGAATGCACAAAAGTGGTTTATCTTGTTCGCTCTCAACTTAGCTTTATGAAATTCATAGCGTCTCACATTCAGAATGATATTGCAAAAGCGATTCAGAGAGACTACTATGTTTACTTTGTTCCTCGTAGGTCTGTTGCGTGTGAGAAGATTCTTGAGCAAGAAAAAGTGCATAACCTGGTTACAGTGAAAGAGTTTCCCTTGTATATGGTTCCACTAGATGAGGATGTTATTTCATTTGAGCTCGAGCTCTCTGAAAAGGATTGCCTTGTGGATGGTGATGTTAGCTCCCTTTGGCATATCGCAAAAGCCATACATGAACTTGAGTTCTCTTTTGGAGTTATATCAAAAATGAGGGCAAAGGGCAAAGCATCAGTTCGTGTTGCAGACATTCTAAACCGCATGCAAGTGGAGGAACCTGTCAATTCGAATGATGTGGGAAGGCCTGAAGTGGATACACTCATTCTATTGGATAGAGAGGTTGACATGGTTACTCCTATGTGTTCCCAGTTAACTTATGAGGGACTAATAGACGAGATTTTGCATATCAGCAATGGAGCTGTCGAGGTCGACTCATCTGTGATGGGTGCTCAGCAGGAGGGAAAAAAGATGAAAGTTCCCCTTAATTCAAGTGATAAGTTGTTCAAGGAGACAAGGGATCTCAACTTTGAAGTCGTTGTACAGGTTCTACGTCAGAAGGCAATGACCATGAAGGAGGACTACACGGAAATTAATTCTACCCAGACAGTTTCTGAGCTGAAGGACTTTGTTAAGAAGCTTAACTCACTACCAGAGATGACAAGGCACATACATCTTGCCCAGCACCTAACGACGTTCACATCCAAGCAATCATTTAATTCTCAACTTGATATGGAACAAACACTTGTTGAGGCAGAAAATTATGACATATGCTATGAGTACATAGAAGAGATGATCCATAAACAGGAGCCTCTCACTAATGTCTTACGTCTATTAGTCTTATTTTCTGTTACAAACTCAGGGTTACCGAAGAAACAATTTGATTATATAAGAATGGAACTACTCCACAGTTATGGGTTTGAGCATGTGGTTACACTGAACAACTTGGAAAAAGCAGGATTGTTGAAGAAGCAGGAGTTTAAAAGCAACTGGCTGACAGTGAAACGGACACTGAAACTTATTGTCGAAGACACTGATACGTCTAGGCCTAACGATATCGCTTATGTCTATTCGGGATATGCTCCTCTTAGCATTCGTCTTATCCAACAAGCTATTCATTCTGGATGGCGTCCTATGGAAGACATTTTGAAGCTGTTACCAGGACCCCATCTGGAGACTAAAAGAAGTGGATTCCCAAGTAGTCCATCAGTTGATTCATTGCACGGAGCTTCAAATGGGGTTGCTGATGGAAGACGCTCAATTGTTCTTGTTGTCTTCATTGGAGGTGTAACATTTGCTGAAATCTCTGCTTTACGGTATCTCGCCTCTAAGGAAGGAATGGCTTATGATTTGATTGTTGCCACAACAAAAATCGTCAACGGCGCAACGTTGATAGAAACATTCATGGAAAAACTAGGCTGA

Protein Analysis

592

Amino Acids

66.91

Weight (kDa)

5.84

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 36 - 601 6e-111 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 789
AciI CCGC 2 cut(s) 206, 619
AclI AACGTT 1 cut(s) 1745
AclWI GGATC 4 cut(s) 4, 116, 885, 1147
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 2 cut(s) 687, 998
AcyI GRCGYC 1 cut(s) 1486
AfaI GTAC 2 cut(s) 900, 1139
AfiI CCNNNNNNNGG 2 cut(s) 19, 1162
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 6 cut(s) 849, 865, 890, 1324, 1507, 1590
AjnI CCWGG 2 cut(s) 396, 1516
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
AleI CACNNNNGTG 1 cut(s) 233
Alw21I GWGCWC 4 cut(s) 75, 465, 471, 811
Alw26I GTCTC 5 cut(s) 148, 288, 315, 863, 1526
AlwI GGATC 4 cut(s) 4, 116, 885, 1147
AlwNI CAGNNNCTG 1 cut(s) 971
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 2 cut(s) 662, 1403
ApoI RAATTY 1 cut(s) 272
AseI ATTAAT 1 cut(s) 951
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 7, 1742
AspS9I GGNCC 1 cut(s) 1520
AsuHPI GGTGA 1 cut(s) 509
AsuII TTCGAA 1 cut(s) 647
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 1 cut(s) 1520
BanII GRGCYC 3 cut(s) 75, 465, 471
BarI GAAGNNNNNNTAC 4 cut(s) 882, 914, 923, 955
BbsI GAAGAC 4 cut(s) 1392, 1503, 1615, 1624
Bbv12I GWGCWC 4 cut(s) 75, 465, 471, 811
BccI CCATC 6 cut(s) 488, 796, 1476, 1533, 1570, 1598
BceAI ACGGC 1 cut(s) 1753
BciT130I CCWGG 2 cut(s) 398, 1518
BciVI GTATCC 1 cut(s) 667
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 5 cut(s) 148, 288, 315, 863, 1526
BfaI CTAG 3 cut(s) 437, 1401, 1772
BfrI CTTAAG 1 cut(s) 170
BfuI GTATCC 1 cut(s) 667
BlpI GCTNAGC 1 cut(s) 810
Bme1390I CCNGG 2 cut(s) 398, 1518
Bme18I GGWCC 1 cut(s) 1520
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 1520
BmiI GGNNCC 5 cut(s) 210, 432, 636, 1167, 1522
BmrFI CCNGG 2 cut(s) 398, 1518
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 1 cut(s) 599
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 4 cut(s) 1392, 1503, 1615, 1624
BplI GAGNNNNNCTC 2 cut(s) 776, 808
BpmI CTGGAG 1 cut(s) 1550
Bpu1102I GCTNAGC 1 cut(s) 810
Bpu14I TTCGAA 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 395, 563
BsaHI GRCGYC 1 cut(s) 1486
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 1162
Bse1I ACTGG 3 cut(s) 212, 727, 1352
Bse3DI GCAATG 3 cut(s) 778, 927, 1574
BseBI CCWGG 2 cut(s) 398, 1518
BseGI GGATG 5 cut(s) 153, 451, 499, 1058, 1487
BseLI CCNNNNNNNGG 2 cut(s) 19, 1162
BseMI GCAATG 3 cut(s) 778, 927, 1574
BseMII CTCAG 5 cut(s) 54, 145, 824, 963, 1230
BseNI ACTGG 3 cut(s) 212, 727, 1352
BseRI GAGGAG 2 cut(s) 21, 1431
BseYI CCCAGC 2 cut(s) 14, 1038
BshFI GGCC 2 cut(s) 664, 1405
BsiHKAI GWGCWC 4 cut(s) 75, 465, 471, 811
BsiHKCI CYCGRG 1 cut(s) 464
BslFI GGGAC 2 cut(s) 68, 756
BslI CCNNNNNNNGG 2 cut(s) 19, 1162
BsmAI GTCTC 5 cut(s) 148, 288, 315, 863, 1526
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 2 cut(s) 68, 756
BsmI GAATGC 3 cut(s) 31, 230, 1449
BsnI GGCC 2 cut(s) 664, 1405
BsoBI CYCGRG 1 cut(s) 464
Bsp119I TTCGAA 1 cut(s) 647
Bsp1286I GDGCHC 4 cut(s) 75, 465, 471, 811
Bsp1407I TGTACA 1 cut(s) 898
Bsp143I GATC 5 cut(s) 9, 121, 150, 877, 1152
Bsp1720I GCTNAGC 1 cut(s) 810
BspACI CCGC 2 cut(s) 206, 619
BspANI GGCC 2 cut(s) 664, 1405
BspCNI CTCAG 5 cut(s) 55, 144, 823, 964, 1229
BspLI GGNNCC 5 cut(s) 210, 432, 636, 1167, 1522
BspPI GGATC 4 cut(s) 4, 116, 885, 1147
BspT104I TTCGAA 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 3 cut(s) 778, 927, 1574
BsrGI TGTACA 1 cut(s) 898
BsrI ACTGG 3 cut(s) 212, 727, 1352
BssMI GATC 5 cut(s) 9, 121, 150, 877, 1152
BssNI GRCGYC 1 cut(s) 1486
Bst2UI CCWGG 2 cut(s) 398, 1518
Bst4CI ACNGT 5 cut(s) 406, 967, 1268, 1357, 1674
Bst6I CTCTTC 1 cut(s) 1140
BstACI GRCGYC 1 cut(s) 1486
BstAFI CTTAAG 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 898
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 1 cut(s) 623
BstEII GGTNACC 1 cut(s) 1221
BstF5I GGATG 5 cut(s) 153, 451, 499, 1058, 1487
BstHHI GCGC 2 cut(s) 7, 1742
BstKTI GATC 5 cut(s) 12, 124, 153, 880, 1155
BstMAI GTCTC 5 cut(s) 148, 288, 315, 863, 1526
BstMBI GATC 5 cut(s) 9, 121, 150, 877, 1152
BstNI CCWGG 2 cut(s) 398, 1518
BstNSI RCATGY 2 cut(s) 625, 1286
BstPI GGTNACC 1 cut(s) 1221
BstSCI CCNGG 2 cut(s) 396, 1516
BstV2I GAAGAC 4 cut(s) 1392, 1503, 1615, 1624
BstX2I RGATCY 2 cut(s) 9, 877
BstXI CCANNNNNNTGG 1 cut(s) 1271
BstYI RGATCY 2 cut(s) 9, 877
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 2 cut(s) 664, 1405
BtsCI GGATG 5 cut(s) 153, 451, 499, 1058, 1487
BtsIMutI CAGTG 6 cut(s) 139, 411, 1292, 1362, 1367, 1389
Cac8I GCNNGC 1 cut(s) 623
CaiI CAGNNNCTG 1 cut(s) 971
CfoI GCGC 2 cut(s) 7, 1742
Cfr13I GGNCC 1 cut(s) 1520
CseI GACGC 3 cut(s) 270, 1475, 1620
CsiI ACCWGGT 1 cut(s) 396
Csp6I GTAC 2 cut(s) 899, 1138
CviAII CATG 6 cut(s) 536, 622, 706, 928, 1283, 1762
CviQI GTAC 2 cut(s) 899, 1138
DpnI GATC 5 cut(s) 11, 123, 152, 879, 1154
DpnII GATC 5 cut(s) 9, 121, 150, 877, 1152
DraI TTTAAA 1 cut(s) 1339
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Ecl136II GAGCTC 3 cut(s) 73, 463, 469
Eco147I AGGCCT 2 cut(s) 664, 1405
Eco24I GRGCYC 3 cut(s) 75, 465, 471
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 1 cut(s) 1520
Eco53kI GAGCTC 3 cut(s) 73, 463, 469
Eco57I CTGAAG 2 cut(s) 687, 998
Eco88I CYCGRG 1 cut(s) 464
Eco91I GGTNACC 1 cut(s) 1221
EcoICRI GAGCTC 3 cut(s) 73, 463, 469
EcoO109I RGGNCCY 1 cut(s) 1520
EcoO65I GGTNACC 1 cut(s) 1221
EcoRII CCWGG 2 cut(s) 396, 1516
EcoRV GATATC 1 cut(s) 1414
EcoT38I GRGCYC 3 cut(s) 75, 465, 471
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 6 cut(s) 539, 625, 709, 931, 1286, 1765
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 2 cut(s) 68, 756
FatI CATG 6 cut(s) 535, 621, 705, 927, 1282, 1761
FauNDI CATATG 1 cut(s) 1127
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 789
FokI GGATG 5 cut(s) 140, 458, 506, 1045, 1494
FriOI GRGCYC 3 cut(s) 75, 465, 471
FspBI CTAG 3 cut(s) 437, 1401, 1772
GlaI GCGC 2 cut(s) 6, 1741
GsaI CCCAGC 2 cut(s) 18, 1042
GsuI CTGGAG 1 cut(s) 1550
HaeIII GGCC 2 cut(s) 664, 1405
HgaI GACGC 3 cut(s) 270, 1475, 1620
HhaI GCGC 2 cut(s) 7, 1742
Hin1I GRCGYC 1 cut(s) 1486
Hin1II CATG 6 cut(s) 539, 625, 709, 931, 1286, 1765
Hin6I GCGC 2 cut(s) 5, 1740
HinP1I GCGC 2 cut(s) 5, 1740
HincII GTYRAC 4 cut(s) 703, 732, 790, 1735
HindII GTYRAC 4 cut(s) 703, 732, 790, 1735
HindIII AAGCTT 2 cut(s) 167, 995
HinfI GANTC 5 cut(s) 313, 370, 791, 1548, 1571
HpaI GTTAAC 1 cut(s) 732
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 7 cut(s) 79, 334, 703, 732, 790, 1056, 1735
Hpy188I TCNGA 8 cut(s) 42, 64, 219, 294, 318, 475, 915, 973
Hpy188III TCNNGA 5 cut(s) 157, 374, 1431, 1479, 1529
Hpy8I GTNNAC 7 cut(s) 79, 334, 703, 732, 790, 1056, 1735
Hpy99I CGWCG 1 cut(s) 1054
HpyAV CCTTC 5 cut(s) 654, 910, 925, 973, 1685
HpyCH4III ACNGT 5 cut(s) 406, 967, 1268, 1357, 1674
HpyCH4IV ACGT 6 cut(s) 93, 910, 1052, 1186, 1397, 1745
HpyCH4V TGCA 7 cut(s) 228, 305, 391, 605, 625, 763, 1579
HpySE526I ACGT 6 cut(s) 93, 910, 1052, 1186, 1397, 1745
Hsp92I GRCGYC 1 cut(s) 1486
Hsp92II CATG 6 cut(s) 539, 625, 709, 931, 1286, 1765
HspAI GCGC 2 cut(s) 5, 1740
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 732
Kzo9I GATC 5 cut(s) 9, 121, 150, 877, 1152
LmnI GCTCC 7 cut(s) 34, 70, 512, 776, 1165, 1444, 1583
LweI GCATC 1 cut(s) 599
MabI ACCWGGT 1 cut(s) 396
MaeI CTAG 3 cut(s) 437, 1401, 1772
MaeII ACGT 6 cut(s) 93, 910, 1052, 1186, 1397, 1745
MaeIII GTNAC 8 cut(s) 189, 400, 709, 1207, 1221, 1288, 1512, 1645
MalI GATC 5 cut(s) 11, 123, 152, 879, 1154
MboI GATC 5 cut(s) 9, 121, 150, 877, 1152
MboII GAAGA 8 cut(s) 379, 1157, 1240, 1336, 1397, 1508, 1620, 1624
MfeI CAATTG 1 cut(s) 1617
MflI RGATCY 2 cut(s) 9, 877
MhlI GDGCHC 4 cut(s) 75, 465, 471, 811
MluCI AATT 9 cut(s) 272, 643, 844, 948, 952, 1075, 1117, 1235, 1617
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 1 cut(s) 1486
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 9 cut(s) 87, 171, 731, 843, 951, 990, 999, 1074, 1338
MslI CAYNNNNRTG 3 cut(s) 233, 626, 1269
MspCI CTTAAG 1 cut(s) 170
MspR9I CCNGG 2 cut(s) 398, 1518
MunI CAATTG 1 cut(s) 1617
Mva1269I GAATGC 3 cut(s) 31, 230, 1449
MvaI CCWGG 2 cut(s) 398, 1518
NdeI CATATG 1 cut(s) 1127
NdeII GATC 5 cut(s) 9, 121, 150, 877, 1152
NlaIII CATG 6 cut(s) 539, 625, 709, 931, 1286, 1765
NlaIV GGNNCC 5 cut(s) 210, 432, 636, 1167, 1522
NspI RCATGY 2 cut(s) 625, 1286
NspV TTCGAA 1 cut(s) 647
OliI CACNNNNGTG 1 cut(s) 233
PaeI GCATGC 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 464
PceI AGGCCT 2 cut(s) 664, 1405
PctI GAATGC 3 cut(s) 31, 230, 1449
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 4 cut(s) 313, 370, 1548, 1571
PleI GAGTC 1 cut(s) 785
PpsI GAGTC 1 cut(s) 785
PpuMI RGGWCCY 1 cut(s) 1520
PshBI ATTAAT 1 cut(s) 951
Psp124BI GAGCTC 3 cut(s) 75, 465, 471
Psp1406I AACGTT 1 cut(s) 1745
Psp5II RGGWCCY 1 cut(s) 1520
Psp6I CCWGG 2 cut(s) 396, 1516
PspEI GGTNACC 1 cut(s) 1221
PspFI CCCAGC 2 cut(s) 14, 1038
PspGI CCWGG 2 cut(s) 396, 1516
PspN4I GGNNCC 5 cut(s) 210, 432, 636, 1167, 1522
PspPI GGNCC 1 cut(s) 1520
PspPPI RGGWCCY 1 cut(s) 1520
PspXI VCTCGAGB 1 cut(s) 464
PstNI CAGNNNCTG 1 cut(s) 971
PsuI RGATCY 2 cut(s) 9, 877
RsaI GTAC 2 cut(s) 900, 1139
RsaNI GTAC 2 cut(s) 899, 1138
RseI CAYNNNNRTG 3 cut(s) 233, 626, 1269
SacI GAGCTC 3 cut(s) 75, 465, 471
SalI GTCGAC 1 cut(s) 788
SaqAI TTAA 9 cut(s) 87, 171, 731, 843, 951, 990, 999, 1074, 1338
Sau3AI GATC 5 cut(s) 9, 121, 150, 877, 1152
Sau96I GGNCC 1 cut(s) 1520
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 2 cut(s) 398, 1518
SduI GDGCHC 4 cut(s) 75, 465, 471, 811
SexAI ACCWGGT 1 cut(s) 396
SfaNI GCATC 1 cut(s) 599
Sfr274I CTCGAG 1 cut(s) 464
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 1 cut(s) 1520
SlaI CTCGAG 1 cut(s) 464
SmiMI CAYNNNNRTG 3 cut(s) 233, 626, 1269
SmlI CTYRAG 4 cut(s) 170, 374, 464, 542
SmoI CTYRAG 4 cut(s) 170, 374, 464, 542
SphI GCATGC 1 cut(s) 625
Sse9I AATT 9 cut(s) 272, 643, 844, 948, 952, 1075, 1117, 1235, 1617
SseBI AGGCCT 2 cut(s) 664, 1405
SsiI CCGC 2 cut(s) 206, 619
SspMI CTAG 3 cut(s) 437, 1401, 1772
SstI GAGCTC 3 cut(s) 75, 465, 471
StuI AGGCCT 2 cut(s) 664, 1405
StyD4I CCNGG 2 cut(s) 396, 1516
TaaI ACNGT 5 cut(s) 406, 967, 1268, 1357, 1674
TaiI ACGT 6 cut(s) 96, 913, 1055, 1189, 1400, 1748
TaqI TCGA 5 cut(s) 465, 647, 783, 789, 1383
TasI AATT 9 cut(s) 272, 643, 844, 948, 952, 1075, 1117, 1235, 1617
TatI WGTACW 2 cut(s) 898, 1137
TfiI GAWTC 4 cut(s) 313, 370, 1548, 1571
Tru1I TTAA 9 cut(s) 87, 171, 731, 843, 951, 990, 999, 1074, 1338
Tru9I TTAA 9 cut(s) 87, 171, 731, 843, 951, 990, 999, 1074, 1338
TscAI CASTG 6 cut(s) 139, 411, 1299, 1362, 1374, 1396
TspGWI ACGGA 3 cut(s) 959, 1378, 1596
TspRI CASTG 6 cut(s) 139, 411, 1299, 1362, 1374, 1396
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 1520
VspI ATTAAT 1 cut(s) 951
XapI RAATTY 1 cut(s) 272
XceI RCATGY 2 cut(s) 625, 1286
XhoI CTCGAG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 3 cut(s) 437, 1401, 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.