Rh7BG430100

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002637
Physical Location & Seq
Reverse (-)
49235826 .. 49238407
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG430100.1

Sequence Viewer

Length: 1800 bp
ATGGCTCAGATTCCCAAATTGGACAATGCTCCTCTCAATCTCAAATATATGAGGGAACAGTCTCAGAAGGAGCTCATAAACATCCTCAAGAATATTCGAGGAAGGAAGTGTTTGGTGATTGACCCCAAGCTCAGCGGTTCAATATCATTGATCATTCAGACTGCAGTACTTCTCAAAGAACTTGAGGTTGAACTGCGACATCTTTCGGCTGAAACAATTCAAACTGACTGTTCCAAAGTGGTTTTCCTTGTCCGTTCTCAGCTTAGTTTGATGAAATACATTAGTTCACAAGTCCATAATGATACTTCGAAAGGGTTACAGAGAGAATACTATATTTACTTTGTCCCTTGCCGCGCAGTTGCTTGTGAGAAGATCCTTGAGGAGGAAAACGTCCATGACTTGTTGAGTATAGGGGAGTACCAGTTATACAGTGTTCCATTGGATGAGGATGTTTTGTCGTTTGAACTTGATCTCTCTCTCAAGGAATGCCTTGTTGATGGTGATATGAGCTCACTTTGGCATATTGCGAAAGCCATTCACAAGCTTGAGTTTTCTTCTGGGGTGATACCAAATGTGAGGGCAAAAGGAAATGCATCGGTGCGTGTTGCTGACATTCTAAACCGCATGCAAGCTGAGGAACCCGTTAGCTCATCAGATATGGTTGTTCCAGAGATAAATACTGTCATCCTTCTAGACAGGGAGGTGGACATGGTTACTCCCATGTGTACTCAGTTAACATATGAGGGCATTATTGATGAGTTTCTGCGTATCAATGATGGTTCTGTTGAGGTTGATCCATCCATCATGGATGTCAAACAAGAGGGAAAGAAGATGAAAGTTCCACTTAATTCAAGTGACAAGCTGTTTAAAGAGATAAGAGATCTCCACTTTGAAGTTGTTGTCCAGATTCTACGCGAAAAAGTAACATCTATGAAGCAGGACTACAATGAGGTGACTTCAAATAAACAGACTCTTTCTGAAATAAAGAATTTTGTCAAAAAGATGCACTCATTGCCAGAAGTGACAAGGCACATAAATCTTGCTAAGCATCTGATGACTTTCACGTCGAAGCCATCATTTCGTGGACTACTTGACATGGAACAAACAATCGTTGAGACTGAGAGTTTTGACATATGCTTAGATTACATTGAAGAAATGATGCATAAGCAGGAGCCTCTTGTAAGTGTCATGCGTCTTCTCATCTTATTTTCTATTACAAATTCTGGGTTGCCCAAAGAGAATTTTGACTATATGAGGAATGAACTACTCCAGAGTTATGGATATGAGCACATGGCTACATTGAAAAATCTGGAGAAGGCAGGATTGTTTAAAAAACAGGAGACAAAAGGCAACTGGCCGAGAGTTAAGCGTGCCTTACAACTTGTCATTGAACACAGTGACACAGCTAACCCCAATGACATTGACTATGTCTTTTCTGGATATGCACCTCTTAGCATTCGCCTTGTTCAGCATGCTATTCGATCTGGATGGCGTCCGATTGAAGAAACTCTAAAGCTGTTACCCGGACCACATTCAGAAACAAAGAGAGGCCCATTCTCAAGCAGCGCATCATTTGACAATTTTCAGGGGGCAGAAACCAATGTAGACAAGGTAGCTGATGGGAGGCGCTCCCTTGTACTTGTCATCTTCATTGGAGGTGTAACGTTTGCAGAGATTTCCGCCCTTCGGTTTCTTAGTTCTCGGGAAGGGATGGCATATGATTTCATAGTTGGGACAACGAAAATGGTTAGTGGCCATAGCTTGACTGAACCATTTGTGGGGAAGATGGAGGACCTGTAA

Protein Analysis

599

Amino Acids

68.01

Weight (kDa)

5.71

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 36 - 589 1.6e-113 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1063
AccI GTMKAC 1 cut(s) 1605
AccII CGCG 2 cut(s) 354, 915
AciI CCGC 4 cut(s) 135, 352, 622, 1680
AclI AACGTT 1 cut(s) 1664
AclWI GGATC 2 cut(s) 367, 788
AcoI YGGCCR 2 cut(s) 1355, 1753
AcsI RAATTY 3 cut(s) 988, 1219, 1240
AcyI GRCGYC 1 cut(s) 1492
AfaI GTAC 4 cut(s) 168, 419, 727, 1638
AfiI CCNNNNNNNGG 3 cut(s) 382, 1686, 1778
AjiI CACGTC 1 cut(s) 1065
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
Alw21I GWGCWC 3 cut(s) 75, 512, 1290
Alw26I GTCTC 3 cut(s) 66, 1109, 1334
AlwI GGATC 2 cut(s) 367, 788
Ama87I CYCGRG 1 cut(s) 1701
AoxI GGCC 3 cut(s) 1355, 1549, 1753
ApeKI GCWGC 1 cut(s) 1563
ApoI RAATTY 3 cut(s) 988, 1219, 1240
Asp700I GAANNNNTTC 1 cut(s) 216
AspLEI GCGC 3 cut(s) 356, 1568, 1629
AspS9I GGNCC 3 cut(s) 1526, 1550, 1792
AsuC2I CCSGG 1 cut(s) 1524
AsuHPI GGTGA 4 cut(s) 127, 512, 574, 964
AsuII TTCGAA 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 1701
AvaII GGWCC 2 cut(s) 1526, 1792
BalI TGGCCA 1 cut(s) 1755
BanII GRGCYC 2 cut(s) 75, 512
BarI GAAGNNNNNNTAC 2 cut(s) 926, 958
BbsI GAAGAC 1 cut(s) 1187
Bbv12I GWGCWC 3 cut(s) 75, 512, 1290
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 1 cut(s) 1575
BccI CCATC 9 cut(s) 491, 770, 805, 809, 1081, 1482, 1613, 1705, 1780
BcgI CGANNNNNNTGC 2 cut(s) 1460, 1494
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 1524
BcoDI GTCTC 3 cut(s) 66, 1109, 1334
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 1630
BglII AGATCT 1 cut(s) 880
BisI GCNGC 2 cut(s) 352, 1564
BlpI GCTNAGC 2 cut(s) 131, 1044
BlsI GCNGC 2 cut(s) 353, 1565
BmcAI AGTACT 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 1524
Bme18I GGWCC 2 cut(s) 1526, 1792
BmeT110I CYCGRG 1 cut(s) 1701
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 3 cut(s) 1526, 1550, 1792
BmiI GGNNCC 2 cut(s) 639, 1173
BmrFI CCNGG 1 cut(s) 1524
BmsI GCATC 5 cut(s) 602, 993, 1057, 1149, 1577
BpiI GAAGAC 1 cut(s) 1187
BpmI CTGGAG 2 cut(s) 1253, 1331
Bpu10I CCTNAGC 1 cut(s) 633
Bpu1102I GCTNAGC 2 cut(s) 131, 1044
Bpu14I TTCGAA 1 cut(s) 308
BpuEI CTTGAG 6 cut(s) 71, 203, 398, 464, 566, 1543
BpuMI CCSGG 1 cut(s) 1524
BsaHI GRCGYC 1 cut(s) 1492
BsaXI ACNNNNNCTCC 3 cut(s) 407, 437, 1781
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 1686, 1778
Bse1I ACTGG 2 cut(s) 421, 1358
Bse3DI GCAATG 1 cut(s) 1010
BseGI GGATG 8 cut(s) 81, 448, 454, 684, 797, 814, 1493, 1716
BseLI CCNNNNNNNGG 3 cut(s) 382, 1686, 1778
BseMI GCAATG 1 cut(s) 1010
BseMII CTCAG 7 cut(s) 20, 77, 145, 272, 624, 743, 1110
BseNI ACTGG 2 cut(s) 421, 1358
BseRI GAGGAG 2 cut(s) 21, 395
BseXI GCAGC 1 cut(s) 1575
Bsh1236I CGCG 2 cut(s) 354, 915
BshFI GGCC 3 cut(s) 1357, 1551, 1755
BsiHKAI GWGCWC 3 cut(s) 75, 512, 1290
BsiHKCI CYCGRG 1 cut(s) 1701
BsiSI CCGG 1 cut(s) 1524
BslFI GGGAC 2 cut(s) 329, 1747
BslI CCNNNNNNNGG 3 cut(s) 382, 1686, 1778
BsmAI GTCTC 3 cut(s) 66, 1109, 1334
BsmFI GGGAC 2 cut(s) 329, 1747
BsmI GAATGC 2 cut(s) 491, 1455
BsnI GGCC 3 cut(s) 1357, 1551, 1755
BsoBI CYCGRG 1 cut(s) 1701
Bsp119I TTCGAA 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 75, 512, 1290
Bsp143I GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
Bsp1720I GCTNAGC 2 cut(s) 131, 1044
BspACI CCGC 4 cut(s) 135, 352, 622, 1680
BspANI GGCC 3 cut(s) 1357, 1551, 1755
BspCNI CTCAG 7 cut(s) 19, 76, 144, 271, 625, 742, 1111
BspFNI CGCG 2 cut(s) 354, 915
BspLI GGNNCC 2 cut(s) 639, 1173
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 2 cut(s) 367, 788
BspT104I TTCGAA 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 1010
BsrI ACTGG 2 cut(s) 421, 1358
BssMI GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
BssNI GRCGYC 1 cut(s) 1492
Bst4CI ACNGT 5 cut(s) 60, 230, 431, 682, 1397
BstACI GRCGYC 1 cut(s) 1492
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 4 cut(s) 626, 630, 1371, 1473
BstENI CCTNNNNNAGG 1 cut(s) 380
BstF5I GGATG 8 cut(s) 81, 448, 454, 684, 797, 814, 1493, 1716
BstFNI CGCG 2 cut(s) 354, 915
BstH2I RGCGCY 1 cut(s) 1630
BstHHI GCGC 3 cut(s) 356, 1568, 1629
BstKTI GATC 6 cut(s) 153, 375, 472, 796, 883, 1484
BstMAI GTCTC 3 cut(s) 66, 1109, 1334
BstMBI GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
BstMWI GCNNNNNNNGC 1 cut(s) 1012
BstNSI RCATGY 2 cut(s) 628, 1475
BstSCI CCNGG 1 cut(s) 1522
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 2 cut(s) 354, 915
BstV1I GCAGC 1 cut(s) 1575
BstV2I GAAGAC 1 cut(s) 1187
BstX2I RGATCY 2 cut(s) 372, 880
BstXI CCANNNNNNTGG 1 cut(s) 1277
BstYI RGATCY 2 cut(s) 372, 880
BsuRI GGCC 3 cut(s) 1357, 1551, 1755
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 8 cut(s) 81, 448, 454, 684, 797, 814, 1493, 1716
BtsIMutI CAGTG 2 cut(s) 436, 1402
Cac8I GCNNGC 4 cut(s) 626, 630, 1371, 1473
CfoI GCGC 3 cut(s) 356, 1568, 1629
Cfr13I GGNCC 3 cut(s) 1526, 1550, 1792
CseI GACGC 2 cut(s) 1181, 1481
Csp6I GTAC 4 cut(s) 167, 418, 726, 1637
CviAII CATG 9 cut(s) 395, 625, 709, 721, 805, 1096, 1189, 1291, 1472
CviQI GTAC 4 cut(s) 167, 418, 726, 1637
DpnI GATC 6 cut(s) 152, 374, 471, 795, 882, 1483
DpnII GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
DraI TTTAAA 2 cut(s) 868, 1330
DrdI GACNNNNNNGTC 1 cut(s) 1063
DseDI GACNNNNNNGTC 1 cut(s) 1063
EaeI YGGCCR 2 cut(s) 1355, 1753
EciI GGCGGA 1 cut(s) 1669
Ecl136II GAGCTC 2 cut(s) 73, 510
Eco24I GRGCYC 2 cut(s) 75, 512
Eco47I GGWCC 2 cut(s) 1526, 1792
Eco53kI GAGCTC 2 cut(s) 73, 510
Eco88I CYCGRG 1 cut(s) 1701
EcoICRI GAGCTC 2 cut(s) 73, 510
EcoNI CCTNNNNNAGG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 1792
EcoT22I ATGCAT 2 cut(s) 595, 1164
EcoT38I GRGCYC 2 cut(s) 75, 512
FaeI CATG 9 cut(s) 398, 628, 712, 724, 808, 1099, 1192, 1294, 1475
FalI AAGNNNNNCTT 4 cut(s) 828, 860, 1358, 1390
FaqI GGGAC 2 cut(s) 329, 1747
FatI CATG 9 cut(s) 394, 624, 708, 720, 804, 1095, 1188, 1290, 1471
FauNDI CATATG 3 cut(s) 739, 1133, 1717
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 1605
Fnu4HI GCNGC 2 cut(s) 352, 1564
FokI GGATG 8 cut(s) 68, 455, 461, 671, 784, 821, 1500, 1723
FriOI GRGCYC 2 cut(s) 75, 512
Fsp4HI GCNGC 2 cut(s) 352, 1564
FspBI CTAG 1 cut(s) 692
GlaI GCGC 3 cut(s) 355, 1567, 1628
GluI GCNGC 2 cut(s) 352, 1564
GsuI CTGGAG 2 cut(s) 1253, 1331
HaeII RGCGCY 1 cut(s) 1630
HaeIII GGCC 3 cut(s) 1357, 1551, 1755
HapII CCGG 1 cut(s) 1524
HgaI GACGC 2 cut(s) 1181, 1481
HhaI GCGC 3 cut(s) 356, 1568, 1629
Hin1I GRCGYC 1 cut(s) 1492
Hin1II CATG 9 cut(s) 398, 628, 712, 724, 808, 1099, 1192, 1294, 1475
Hin6I GCGC 3 cut(s) 354, 1566, 1627
HinP1I GCGC 3 cut(s) 354, 1566, 1627
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 3 cut(s) 10, 907, 970
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 1524
HphI GGTGA 4 cut(s) 127, 512, 574, 964
Hpy166II GTNNAC 6 cut(s) 287, 706, 726, 735, 1085, 1606
Hpy188I TCNGA 8 cut(s) 9, 66, 159, 655, 979, 1053, 1497, 1537
Hpy188III TCNNGA 9 cut(s) 88, 668, 692, 904, 1270, 1310, 1437, 1485, 1703
Hpy8I GTNNAC 6 cut(s) 287, 706, 726, 735, 1085, 1606
Hpy99I CGWCG 1 cut(s) 1069
HpyAV CCTTC 6 cut(s) 61, 96, 698, 1309, 1694, 1700
HpyCH4III ACNGT 5 cut(s) 60, 230, 431, 682, 1397
HpyCH4IV ACGT 3 cut(s) 390, 1064, 1664
HpyCH4V TGCA 7 cut(s) 164, 593, 628, 1006, 1162, 1445, 1670
HpyF10VI GCNNNNNNNGC 1 cut(s) 1012
HpySE526I ACGT 3 cut(s) 390, 1064, 1664
Hsp92I GRCGYC 1 cut(s) 1492
Hsp92II CATG 9 cut(s) 398, 628, 712, 724, 808, 1099, 1192, 1294, 1475
HspAI GCGC 3 cut(s) 354, 1566, 1627
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
LmnI GCTCC 4 cut(s) 34, 70, 1171, 1634
Lsp1109I GCAGC 1 cut(s) 1575
LweI GCATC 5 cut(s) 602, 993, 1057, 1149, 1577
MaeI CTAG 1 cut(s) 692
MaeII ACGT 3 cut(s) 390, 1064, 1664
MaeIII GTNAC 9 cut(s) 315, 712, 854, 922, 952, 1021, 1397, 1518, 1660
MalI GATC 6 cut(s) 152, 374, 471, 795, 882, 1483
MboI GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
MboII GAAGA 8 cut(s) 382, 546, 841, 1163, 1187, 1514, 1639, 1795
MflI RGATCY 2 cut(s) 372, 880
MhlI GDGCHC 3 cut(s) 75, 512, 1290
MlsI TGGCCA 1 cut(s) 1755
MluCI AATT 7 cut(s) 17, 216, 847, 988, 1219, 1240, 1579
MluNI TGGCCA 1 cut(s) 1755
MlyI GAGTC 1 cut(s) 964
Mox20I TGGCCA 1 cut(s) 1755
Mph1103I ATGCAT 2 cut(s) 595, 1164
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 1755
MseI TTAA 5 cut(s) 734, 846, 867, 1329, 1365
Msp20I TGGCCA 1 cut(s) 1755
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 1 cut(s) 1524
MspR9I CCNGG 1 cut(s) 1524
Mva1269I GAATGC 2 cut(s) 491, 1455
MvnI CGCG 2 cut(s) 354, 915
MwoI GCNNNNNNNGC 1 cut(s) 1012
NciI CCSGG 1 cut(s) 1524
NdeI CATATG 3 cut(s) 739, 1133, 1717
NdeII GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
NlaIII CATG 9 cut(s) 398, 628, 712, 724, 808, 1099, 1192, 1294, 1475
NlaIV GGNNCC 2 cut(s) 639, 1173
NmeAIII GCCGAG 1 cut(s) 1383
NmuCI GTSAC 4 cut(s) 854, 952, 1021, 1397
NsiI ATGCAT 2 cut(s) 595, 1164
NspI RCATGY 2 cut(s) 628, 1475
NspV TTCGAA 1 cut(s) 308
PaeI GCATGC 2 cut(s) 628, 1475
PctI GAATGC 2 cut(s) 491, 1455
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 10, 907
PflFI GACNNNGTC 1 cut(s) 1427
PkrI GCNGC 2 cut(s) 353, 1565
PleI GAGTC 1 cut(s) 964
PpsI GAGTC 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 1792
Psp124BI GAGCTC 2 cut(s) 75, 512
Psp1406I AACGTT 1 cut(s) 1664
Psp5II RGGWCCY 1 cut(s) 1792
PspN4I GGNNCC 2 cut(s) 639, 1173
PspPI GGNCC 3 cut(s) 1526, 1550, 1792
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 2 cut(s) 372, 880
PsyI GACNNNGTC 1 cut(s) 1427
RsaI GTAC 4 cut(s) 168, 419, 727, 1638
RsaNI GTAC 4 cut(s) 167, 418, 726, 1637
SacI GAGCTC 2 cut(s) 75, 512
SaqAI TTAA 5 cut(s) 734, 846, 867, 1329, 1365
SatI GCNGC 2 cut(s) 352, 1564
Sau3AI GATC 6 cut(s) 150, 372, 469, 793, 880, 1481
Sau96I GGNCC 3 cut(s) 1526, 1550, 1792
ScaI AGTACT 1 cut(s) 168
SchI GAGTC 1 cut(s) 964
ScrFI CCNGG 1 cut(s) 1524
SduI GDGCHC 3 cut(s) 75, 512, 1290
SfaNI GCATC 5 cut(s) 602, 993, 1057, 1149, 1577
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 308
SinI GGWCC 2 cut(s) 1526, 1792
SmlI CTYRAG 6 cut(s) 86, 182, 377, 479, 545, 1558
SmoI CTYRAG 6 cut(s) 86, 182, 377, 479, 545, 1558
SphI GCATGC 2 cut(s) 628, 1475
Sse9I AATT 7 cut(s) 17, 216, 847, 988, 1219, 1240, 1579
SsiI CCGC 4 cut(s) 135, 352, 622, 1680
SspI AATATT 1 cut(s) 94
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 2 cut(s) 75, 512
StyD4I CCNGG 1 cut(s) 1522
TaaI ACNGT 5 cut(s) 60, 230, 431, 682, 1397
TaiI ACGT 3 cut(s) 393, 1067, 1667
TaqI TCGA 4 cut(s) 97, 308, 1067, 1480
TasI AATT 7 cut(s) 17, 216, 847, 988, 1219, 1240, 1579
TatI WGTACW 3 cut(s) 166, 725, 1636
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 2 cut(s) 10, 907
Tru1I TTAA 5 cut(s) 734, 846, 867, 1329, 1365
Tru9I TTAA 5 cut(s) 734, 846, 867, 1329, 1365
TscAI CASTG 2 cut(s) 436, 1402
TseFI GTSAC 4 cut(s) 854, 952, 1021, 1397
TseI GCWGC 1 cut(s) 1563
Tsp45I GTSAC 4 cut(s) 854, 952, 1021, 1397
TspDTI ATGAA 6 cut(s) 287, 848, 947, 1275, 1639, 1714
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 2 cut(s) 436, 1402
Tth111I GACNNNGTC 1 cut(s) 1427
VpaK11BI GGWCC 2 cut(s) 1526, 1792
XagI CCTNNNNNAGG 1 cut(s) 380
XapI RAATTY 3 cut(s) 988, 1219, 1240
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 2 cut(s) 628, 1475
XmiI GTMKAC 1 cut(s) 1605
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 1 cut(s) 692
ZrmI AGTACT 1 cut(s) 168
Zsp2I ATGCAT 2 cut(s) 595, 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.