RchiOBHm_Chr6g0252451

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002637
Physical Location & Seq
Forward (+)
7611193 .. 7611597
405 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22636

Sequence Viewer

Length: 405 bp
ATGAAATACATTAGTTCACAAGTCCATAATGATACTTCGAAAGGGTTACAGAGAGAATACTATCTTTACTTTGTCCCTTGCTGCGCAGTTGCTTGTGAGAATATCCTTGAGGAGGAAAAAGTCCATGACTTGTTGAGTATAGGGGAGTACCAGCTATGCAGTGTTCCATTGGATGAGGATGTTTTGTCATTTGAACTTGATCTCTCTCTCAAGGAATGCCTTGTTGATGGTGATATGAGCTCACTTTGGCATATTGCGAAAGCCATTCACAAGCTTGAGTTTTCTTTTGGGGTGATACCAAATGTGAGGGCGAAAGGAAATGCATCGGTGTGTGTTGCTGACATTCTAAACGCATGCAAGCTTAGGAACCCGTTAGCTCATCAAACATGGTTGTTCCAAAGATAA

Protein Analysis

134

Amino Acids

15.19

Weight (kDa)

5.26

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 4 - 116 8.5e-14 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 85
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 5 cut(s) 154, 240, 274, 361, 377
AluI AGCT 5 cut(s) 154, 240, 274, 361, 377
Alw21I GWGCWC 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 81
AspLEI GCGC 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 242, 304
AsuII TTCGAA 1 cut(s) 38
BanII GRGCYC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 68
BccI CCATC 1 cut(s) 221
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
BmiI GGNNCC 1 cut(s) 368
BmsI GCATC 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 362
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 3 cut(s) 128, 194, 296
Bsc4I CCNNNNNNNGG 1 cut(s) 112
BseGI GGATG 2 cut(s) 178, 184
BseLI CCNNNNNNNGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 242
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 368
BspT104I TTCGAA 1 cut(s) 38
BssMI GATC 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 355, 359
BstDEI CTNAG 1 cut(s) 362
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 178, 184
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 357
BstV1I GCAGC 1 cut(s) 68
BtsCI GGATG 2 cut(s) 178, 184
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 2 cut(s) 355, 359
CfoI GCGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 125, 354, 387
CviJI RGCY 6 cut(s) 154, 240, 263, 274, 361, 377
CviKI_1 RGCY 6 cut(s) 154, 240, 263, 274, 361, 377
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 362
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 242
FaeI CATG 3 cut(s) 128, 357, 390
FaiI YATR 8 cut(s) 27, 126, 140, 157, 236, 252, 355, 388
FaqI GGGAC 1 cut(s) 59
FatI CATG 3 cut(s) 124, 353, 386
Fnu4HI GCNGC 1 cut(s) 82
FokI GGATG 2 cut(s) 185, 191
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 1 cut(s) 82
FspI TGCGCA 1 cut(s) 85
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 128, 357, 390
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HindIII AAGCTT 2 cut(s) 272, 359
HphI GGTGA 2 cut(s) 242, 304
Hpy166II GTNNAC 1 cut(s) 17
Hpy8I GTNNAC 1 cut(s) 17
HpyCH4V TGCA 3 cut(s) 159, 323, 357
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 3 cut(s) 128, 357, 390
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 1 cut(s) 199
LpnPI CCDG 1 cut(s) 164
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 45
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 242
MnlI CCTC 4 cut(s) 103, 106, 169, 300
Mph1103I ATGCAT 1 cut(s) 325
MslI CAYNNNNRTG 1 cut(s) 328
Mva1269I GAATGC 1 cut(s) 221
NdeII GATC 1 cut(s) 199
NlaIII CATG 3 cut(s) 128, 357, 390
NlaIV GGNNCC 1 cut(s) 368
NsbI TGCGCA 1 cut(s) 85
NsiI ATGCAT 1 cut(s) 325
NspI RCATGY 1 cut(s) 357
NspV TTCGAA 1 cut(s) 38
PaeI GCATGC 1 cut(s) 357
PctI GAATGC 1 cut(s) 221
PkrI GCNGC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 368
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 328
SacI GAGCTC 1 cut(s) 242
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 1 cut(s) 199
SduI GDGCHC 1 cut(s) 242
SetI ASST 5 cut(s) 156, 242, 276, 363, 379
SfaNI GCATC 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 328
SmlI CTYRAG 3 cut(s) 107, 209, 275
SmoI CTYRAG 3 cut(s) 107, 209, 275
SphI GCATGC 1 cut(s) 357
SstI GAGCTC 1 cut(s) 242
TaqI TCGA 1 cut(s) 38
TscAI CASTG 1 cut(s) 166
TseI GCWGC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 110
XceI RCATGY 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.