Rh7CG477700

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002637
Physical Location & Seq
Reverse (-)
64146534 .. 64147250
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG477700.1

Sequence Viewer

Length: 717 bp
ATGAAGAGGACCCAAACGGCACCGTATTCTCTACTACTGCCACATATAGTCCGATTCATCTTTTATGTACGAAAGGTGGAGTCTTTGGATTTGTGTGCTGAAGCAGGTTTGGGAAGAAGACGATCGAGTTTGATTTCGAGGGCATTGCGATGGTTTTTGGTCTCCACTTTAAATTGGATTACTTCACCCCAACTTGATTTTCAGGTACCCTTCATTTTCCATCTTTTCCATTTTGAGGTTCGGCTACCGAATTTTAGAGGTTCGAATTTGATGGCTCAGATTCCCAAATTGGACGATGCTCCTCTCAATTTCAAATATATGAGGGAACAGTCTCAGAAGGAGCTCATAAACATCCTCAAGAATATTCGAGGAAGGAAGTGTTTGGTGATTGACCCCAACCTCAGCGGTTCAATATCATTGATCATTCAGACTGCACTTCTCAAAGAACTTGTGGTTGACCTGCGACATCTTTCGGCTGAAACAATTCAAACTGACTGTTCCAAAGTGGTTTTCCTTGTCCGTTCTCAGCTTAGTTTGATGAAATACATTAGTTCACAAGTCCATAATGATACCTCGAAAGGGTTACAGAGAGAATACTATCTTTACTTTGTCCCTTGCCGCGCAGTTGCTTGTGAGAAGATCCTTGAGGAGGAAAAAGTCCATGGCTTGTTGAGTATAGGGGAGTACCAGTTATACAGTACGTGTTCCATTGGATGA

Protein Analysis

238

Amino Acids

27.47

Weight (kDa)

9.33

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 126 - 221 1e-09 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 95, 468
Acc65I GGTACC 1 cut(s) 205
AccB1I GGYRCC 2 cut(s) 19, 205
AccII CGCG 1 cut(s) 621
AciI CCGC 2 cut(s) 405, 619
AclWI GGATC 1 cut(s) 634
AcsI RAATTY 2 cut(s) 250, 265
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 4 cut(s) 69, 207, 686, 700
AfiI CCNNNNNNNGG 3 cut(s) 235, 579, 649
AflIII ACRYGT 1 cut(s) 701
AgsI TTSAA 3 cut(s) 313, 411, 488
AjuI GAANNNNNNNTTGG 4 cut(s) 183, 215, 494, 526
AluBI AGCT 2 cut(s) 343, 529
AluI AGCT 2 cut(s) 343, 529
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 166, 336
AlwI GGATC 1 cut(s) 634
ApoI RAATTY 2 cut(s) 250, 265
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 205
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 1 cut(s) 9
AsuHPI GGTGA 2 cut(s) 177, 397
AsuII TTCGAA 1 cut(s) 263
AvaII GGWCC 1 cut(s) 9
BanI GGYRCC 2 cut(s) 19, 205
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 401
BccI CCATC 3 cut(s) 144, 228, 265
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 2 cut(s) 166, 336
BfuAI ACCTGC 2 cut(s) 95, 468
BisI GCNGC 1 cut(s) 619
BlsI GCNGC 1 cut(s) 620
Bme18I GGWCC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 3 cut(s) 11, 21, 207
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 401
Bpu14I TTCGAA 1 cut(s) 263
BpuEI CTTGAG 2 cut(s) 341, 665
BsaAI YACGTR 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 674, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 579, 649
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 661
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 3 cut(s) 235, 579, 649
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 4 cut(s) 290, 347, 415, 539
BseNI ACTGG 1 cut(s) 688
BseRI GAGGAG 2 cut(s) 291, 662
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 621
Bsh1285I CGRYCG 1 cut(s) 125
BshNI GGYRCC 2 cut(s) 19, 205
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 345
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 3 cut(s) 235, 579, 649
BsmAI GTCTC 2 cut(s) 166, 336
BsmFI GGGAC 1 cut(s) 596
Bso31I GGTCTC 1 cut(s) 166
Bsp119I TTCGAA 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 3 cut(s) 122, 420, 639
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 2 cut(s) 405, 619
BspCNI CTCAG 4 cut(s) 289, 346, 414, 538
BspFNI CGCG 1 cut(s) 621
BspLI GGNNCC 3 cut(s) 11, 21, 207
BspMI ACCTGC 2 cut(s) 95, 468
BspPI GGATC 1 cut(s) 634
BspT104I TTCGAA 1 cut(s) 263
BspT107I GGYRCC 2 cut(s) 19, 205
BspTNI GGTCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 143
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 1 cut(s) 661
BssMI GATC 3 cut(s) 122, 420, 639
BssT1I CCWWGG 1 cut(s) 661
Bst4CI ACNGT 4 cut(s) 24, 330, 497, 698
BstBAI YACGTR 1 cut(s) 702
BstBI TTCGAA 1 cut(s) 263
BstDEI CTNAG 5 cut(s) 276, 333, 401, 525, 530
BstDSI CCRYGG 1 cut(s) 661
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 1 cut(s) 621
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 3 cut(s) 125, 423, 642
BstMAI GTCTC 2 cut(s) 166, 336
BstMBI GATC 3 cut(s) 122, 420, 639
BstMCI CGRYCG 1 cut(s) 125
BstUI CGCG 1 cut(s) 621
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 639
BstYI RGATCY 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 661
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 1 cut(s) 351
BveI ACCTGC 2 cut(s) 95, 468
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 9
Csp6I GTAC 4 cut(s) 68, 206, 685, 699
CviAII CATG 1 cut(s) 662
CviJI RGCY 6 cut(s) 244, 275, 343, 476, 529, 666
CviKI_1 RGCY 6 cut(s) 244, 275, 343, 476, 529, 666
CviQI GTAC 4 cut(s) 68, 206, 685, 699
DdeI CTNAG 5 cut(s) 276, 333, 401, 525, 530
DpnI GATC 3 cut(s) 124, 422, 641
DpnII GATC 3 cut(s) 122, 420, 639
DraI TTTAAA 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 661
FaeI CATG 1 cut(s) 665
FaqI GGGAC 1 cut(s) 596
FatI CATG 1 cut(s) 661
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 619
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 619
GlaI GCGC 1 cut(s) 622
GluI GCNGC 1 cut(s) 619
HhaI GCGC 1 cut(s) 623
Hin1II CATG 1 cut(s) 665
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 3 cut(s) 54, 80, 280
HphI GGTGA 2 cut(s) 177, 397
Hpy166II GTNNAC 2 cut(s) 457, 554
Hpy188I TCNGA 4 cut(s) 53, 279, 336, 429
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 2 cut(s) 457, 554
HpyAV CCTTC 3 cut(s) 220, 331, 366
HpyCH4III ACNGT 4 cut(s) 24, 330, 497, 698
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 1 cut(s) 434
HpyF3I CTNAG 5 cut(s) 276, 333, 401, 525, 530
HpySE526I ACGT 1 cut(s) 701
Hsp92II CATG 1 cut(s) 665
HspAI GCGC 1 cut(s) 621
KpnI GGTACC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 3 cut(s) 122, 420, 639
LmnI GCTCC 2 cut(s) 304, 340
LpnPI CCDG 4 cut(s) 90, 188, 473, 701
LweI GCATC 1 cut(s) 286
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 1 cut(s) 582
MalI GATC 3 cut(s) 124, 422, 641
MboI GATC 3 cut(s) 122, 420, 639
MboII GAAGA 4 cut(s) 16, 126, 129, 649
MflI RGATCY 1 cut(s) 639
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 6 cut(s) 172, 250, 265, 287, 307, 483
MlyI GAGTC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 148
MspA1I CMGCKG 1 cut(s) 405
MvnI CGCG 1 cut(s) 621
NcoI CCATGG 1 cut(s) 661
NdeII GATC 3 cut(s) 122, 420, 639
NlaIII CATG 1 cut(s) 665
NlaIV GGNNCC 3 cut(s) 11, 21, 207
NspV TTCGAA 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 54, 280
PkrI GCNGC 1 cut(s) 620
Ple19I CGATCG 1 cut(s) 125
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 702
PpuMI RGGWCCY 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 11, 21, 207
PspPI GGNCC 1 cut(s) 9
PspPPI RGGWCCY 1 cut(s) 9
PsuI RGATCY 1 cut(s) 639
PvuI CGATCG 1 cut(s) 125
RsaI GTAC 4 cut(s) 69, 207, 686, 700
RsaNI GTAC 4 cut(s) 68, 206, 685, 699
RseI CAYNNNNRTG 1 cut(s) 148
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 1 cut(s) 619
Sau3AI GATC 3 cut(s) 122, 420, 639
Sau96I GGNCC 1 cut(s) 9
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 1 cut(s) 286
SfuI TTCGAA 1 cut(s) 263
SinI GGWCC 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 356, 644
SmoI CTYRAG 2 cut(s) 356, 644
Sse9I AATT 6 cut(s) 172, 250, 265, 287, 307, 483
SsiI CCGC 2 cut(s) 405, 619
SspI AATATT 1 cut(s) 364
SstI GAGCTC 1 cut(s) 345
StyI CCWWGG 1 cut(s) 661
TaaI ACNGT 4 cut(s) 24, 330, 497, 698
TaiI ACGT 1 cut(s) 704
TaqI TCGA 5 cut(s) 125, 137, 263, 367, 575
TasI AATT 6 cut(s) 172, 250, 265, 287, 307, 483
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 2 cut(s) 54, 280
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TspDTI ATGAA 4 cut(s) 17, 46, 202, 554
TspGWI ACGGA 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 2 cut(s) 250, 265
XmnI GAANNNNTTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.