Rh7DG446800

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004574
Physical Location & Seq
Forward (+)
63402570 .. 63405080
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG446800.1

Sequence Viewer

Length: 759 bp
ATGGCGTCCTCCATACCCGATAACCTAACCAGAGACCAGTACGTGTACCTGGCCAAGCTCGCCGAGCAGGCCGAGCGCTACGAGGAGATGGTCCAGTTCATGGAGAAGCTCGTCGTGGGGTCCACTCCGGCGTCGGAGCTCACCGTCGAGGAGCGCAACCTCCTCTCCGTCGCCTACAAGAACGTGATCGGGTCGCTCCGGGCCGCCTGGCGGATCGTGTCGTCGATCGAGCAGAAGGAGGAGGGCCGCAAGAACGAGGAGCACGTGGCGCTCGTCAAGGAGTACAGATCGAAGGTCGAGGCCGAGCTCTCTGAGGTCTGCGCCAGCATTCTGAGGATCCTGGACTCCAATCTGGTGCCGTCGGCGTCGGCGAGCGAGTCGAAGGTGTTCTATTTGAAGATGAAGGGGGACTACCACCGCTACTTGGCGGAGTTCAAGTCGGCCGAGGAGAGGAAGGCTGCTGCTGAGGACACCATGCTCTCCTACAAGGCTGCTCAGGATGTTGCTGTTGCGGATCTGGCACCAACTCACCCCATAAGATTGGGTCTAGCTCTGAACTTCTCAGTGTTCTACTATGAGATTCTCAATCAGTCTGACAAAGCTTGTAGCATGGCCAAACAGGCATTTGAGGAAGCCATTGCTGAGCTAGACACTTTGGGTGAAGAATCTTACAAGGACAGCACTCTCATCATGCAACTTTTGAGGGACAACCTCACCCTCTGGACTTCAGATGTGACGGAAGGGTTAGATGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

252

Amino Acids

28.34

Weight (kDa)

4.75

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 242 3e-99 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 355, 520
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 6 cut(s) 204, 211, 247, 418, 428, 512
AclWI GGATC 4 cut(s) 221, 331, 344, 522
AcoI YGGCCR 3 cut(s) 51, 441, 612
AcuI CTGAAG 1 cut(s) 711
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 3 cut(s) 5, 131, 365
AfaI GTAC 3 cut(s) 41, 47, 284
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 4 cut(s) 100, 210, 424, 450
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 2 cut(s) 397, 436
AjnI CCWGG 3 cut(s) 48, 206, 339
AluBI AGCT 7 cut(s) 58, 109, 139, 307, 551, 602, 646
AluI AGCT 7 cut(s) 58, 109, 139, 307, 551, 602, 646
Alw21I GWGCWC 3 cut(s) 141, 264, 309
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 4 cut(s) 221, 331, 344, 522
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 7 cut(s) 51, 69, 201, 244, 300, 441, 612
ApeKI GCWGC 3 cut(s) 458, 461, 491
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 4 cut(s) 78, 156, 271, 323
AspS9I GGNCC 4 cut(s) 91, 120, 201, 244
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 4 cut(s) 133, 521, 671, 706
AvaII GGWCC 2 cut(s) 91, 120
BalI TGGCCA 2 cut(s) 53, 614
BamHI GGATCC 1 cut(s) 336
BanI GGYRCC 2 cut(s) 355, 520
BanII GRGCYC 2 cut(s) 141, 309
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 3 cut(s) 141, 264, 309
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 445, 448, 478
BccI CCATC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 3 cut(s) 50, 208, 341
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 548, 647
BfoI RGCGCY 2 cut(s) 79, 272
BglI GCCNNNNNGGC 2 cut(s) 68, 620
BisI GCNGC 5 cut(s) 204, 247, 459, 462, 492
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 5 cut(s) 205, 248, 460, 463, 493
Bme1390I CCNGG 4 cut(s) 50, 200, 208, 341
Bme18I GGWCC 2 cut(s) 91, 120
BmgT120I GGNCC 4 cut(s) 91, 120, 201, 244
BmiI GGNNCC 4 cut(s) 121, 338, 357, 522
BmrFI CCNGG 4 cut(s) 50, 200, 208, 341
Bpu10I CCTNAGC 2 cut(s) 465, 495
Bpu1102I GCTNAGC 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 200
BsaAI YACGTR 2 cut(s) 43, 265
BsaHI GRCGYC 3 cut(s) 5, 131, 365
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 444
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 422, 452
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 210, 424, 450
Bse1I ACTGG 2 cut(s) 37, 94
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 3 cut(s) 50, 208, 341
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 4 cut(s) 100, 210, 424, 450
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 6 cut(s) 303, 323, 456, 509, 576, 633
BseNI ACTGG 2 cut(s) 37, 94
BseRI GAGGAG 6 cut(s) 98, 152, 164, 254, 272, 461
BseX3I CGGCCG 1 cut(s) 441
BseXI GCAGC 3 cut(s) 445, 448, 478
Bsh1285I CGRYCG 2 cut(s) 228, 444
BshFI GGCC 7 cut(s) 53, 71, 203, 246, 302, 443, 614
BshNI GGYRCC 2 cut(s) 355, 520
BsiEI CGRYCG 2 cut(s) 228, 444
BsiHKAI GWGCWC 3 cut(s) 141, 264, 309
BsiSI CCGG 2 cut(s) 128, 199
BslFI GGGAC 2 cut(s) 422, 719
BslI CCNNNNNNNGG 4 cut(s) 100, 210, 424, 450
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 422, 719
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 7 cut(s) 53, 71, 203, 246, 302, 443, 614
Bso31I GGTCTC 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 141, 264, 309
Bsp143I GATC 6 cut(s) 186, 213, 225, 287, 336, 514
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 6 cut(s) 204, 211, 247, 418, 428, 512
BspANI GGCC 7 cut(s) 53, 71, 203, 246, 302, 443, 614
BspCNI CTCAG 6 cut(s) 304, 324, 457, 508, 575, 634
BspLI GGNNCC 4 cut(s) 121, 338, 357, 522
BspPI GGATC 4 cut(s) 221, 331, 344, 522
BspT107I GGYRCC 2 cut(s) 355, 520
BspTNI GGTCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 2 cut(s) 37, 94
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 6 cut(s) 186, 213, 225, 287, 336, 514
BssNI GRCGYC 3 cut(s) 5, 131, 365
Bst2UI CCWGG 3 cut(s) 50, 208, 341
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 3 cut(s) 5, 131, 365
BstBAI YACGTR 2 cut(s) 43, 265
BstC8I GCNNGC 4 cut(s) 60, 69, 325, 373
BstDEI CTNAG 6 cut(s) 312, 332, 465, 495, 562, 642
BstF5I GGATG 1 cut(s) 505
BstH2I RGCGCY 2 cut(s) 79, 272
BstHHI GCGC 4 cut(s) 78, 156, 271, 323
BstKTI GATC 6 cut(s) 189, 216, 228, 290, 339, 517
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 6 cut(s) 186, 213, 225, 287, 336, 514
BstMCI CGRYCG 2 cut(s) 228, 444
BstMWI GCNNNNNNNGC 7 cut(s) 59, 64, 68, 73, 268, 518, 620
BstNI CCWGG 3 cut(s) 50, 208, 341
BstSCI CCNGG 4 cut(s) 48, 198, 206, 339
BstV1I GCAGC 3 cut(s) 445, 448, 478
BstX2I RGATCY 2 cut(s) 336, 514
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 2 cut(s) 336, 514
BstZI CGGCCG 1 cut(s) 441
BsuRI GGCC 7 cut(s) 53, 71, 203, 246, 302, 443, 614
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 4 cut(s) 60, 69, 325, 373
CfoI GCGC 4 cut(s) 78, 156, 271, 323
Cfr13I GGNCC 4 cut(s) 91, 120, 201, 244
CseI GACGC 2 cut(s) 120, 354
Csp6I GTAC 3 cut(s) 40, 46, 283
CviAII CATG 4 cut(s) 100, 475, 610, 691
CviQI GTAC 3 cut(s) 40, 46, 283
DdeI CTNAG 6 cut(s) 312, 332, 465, 495, 562, 642
DpnI GATC 6 cut(s) 188, 215, 227, 289, 338, 516
DpnII GATC 6 cut(s) 186, 213, 225, 287, 336, 514
EaeI YGGCCR 3 cut(s) 51, 441, 612
EagI CGGCCG 1 cut(s) 441
EciI GGCGGA 2 cut(s) 226, 443
Ecl136II GAGCTC 2 cut(s) 139, 307
EclXI CGGCCG 1 cut(s) 441
Eco24I GRGCYC 2 cut(s) 141, 309
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 91, 120
Eco47III AGCGCT 1 cut(s) 77
Eco52I CGGCCG 1 cut(s) 441
Eco53kI GAGCTC 2 cut(s) 139, 307
Eco57I CTGAAG 1 cut(s) 711
Eco72I CACGTG 1 cut(s) 265
EcoICRI GAGCTC 2 cut(s) 139, 307
EcoRII CCWGG 3 cut(s) 48, 206, 339
EcoT38I GRGCYC 2 cut(s) 141, 309
FaeI CATG 4 cut(s) 103, 478, 613, 694
FaiI YATR 8 cut(s) 14, 101, 476, 536, 576, 611, 692, 757
FaqI GGGAC 2 cut(s) 422, 719
FatI CATG 4 cut(s) 99, 474, 609, 690
Fnu4HI GCNGC 5 cut(s) 204, 247, 459, 462, 492
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 2 cut(s) 141, 309
Fsp4HI GCNGC 5 cut(s) 204, 247, 459, 462, 492
FspBI CTAG 2 cut(s) 548, 647
GlaI GCGC 4 cut(s) 77, 155, 270, 322
GluI GCNGC 5 cut(s) 204, 247, 459, 462, 492
HaeII RGCGCY 2 cut(s) 79, 272
HaeIII GGCC 7 cut(s) 53, 71, 203, 246, 302, 443, 614
HapII CCGG 2 cut(s) 128, 199
HgaI GACGC 2 cut(s) 120, 354
HhaI GCGC 4 cut(s) 78, 156, 271, 323
Hin1I GRCGYC 3 cut(s) 5, 131, 365
Hin1II CATG 4 cut(s) 103, 478, 613, 694
Hin6I GCGC 4 cut(s) 76, 154, 269, 321
HinP1I GCGC 4 cut(s) 76, 154, 269, 321
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 4 cut(s) 344, 377, 580, 665
HpaII CCGG 2 cut(s) 128, 199
HphI GGTGA 4 cut(s) 133, 521, 671, 706
Hpy166II GTNNAC 2 cut(s) 46, 123
Hpy188I TCNGA 6 cut(s) 136, 313, 333, 555, 595, 730
Hpy188III TCNNGA 2 cut(s) 497, 721
Hpy8I GTNNAC 2 cut(s) 46, 123
Hpy99I CGWCG 7 cut(s) 116, 136, 149, 173, 226, 364, 370
HpyAV CCTTC 6 cut(s) 229, 286, 376, 397, 448, 734
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 42, 183, 264
HpyCH4V TGCA 1 cut(s) 694
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 64, 68, 73, 268, 518, 620
HpyF3I CTNAG 6 cut(s) 312, 332, 465, 495, 562, 642
HpySE526I ACGT 3 cut(s) 42, 183, 264
Hsp92I GRCGYC 3 cut(s) 5, 131, 365
Hsp92II CATG 4 cut(s) 103, 478, 613, 694
HspAI GCGC 4 cut(s) 76, 154, 269, 321
Kzo9I GATC 6 cut(s) 186, 213, 225, 287, 336, 514
LmnI GCTCC 4 cut(s) 136, 151, 201, 259
Lsp1109I GCAGC 3 cut(s) 445, 448, 478
MaeI CTAG 2 cut(s) 548, 647
MaeII ACGT 3 cut(s) 42, 183, 264
MaeIII GTNAC 1 cut(s) 733
MalI GATC 6 cut(s) 188, 215, 227, 289, 338, 516
MboI GATC 6 cut(s) 186, 213, 225, 287, 336, 514
MboII GAAGA 2 cut(s) 409, 674
MflI RGATCY 2 cut(s) 336, 514
MhlI GDGCHC 3 cut(s) 141, 264, 309
MlsI TGGCCA 2 cut(s) 53, 614
MluNI TGGCCA 2 cut(s) 53, 614
MlyI GAGTC 2 cut(s) 338, 386
MmeI TCCRAC 1 cut(s) 114
Mox20I TGGCCA 2 cut(s) 53, 614
MroXI GAANNNNTTC 1 cut(s) 386
MscI TGGCCA 2 cut(s) 53, 614
Msp20I TGGCCA 2 cut(s) 53, 614
MspI CCGG 2 cut(s) 128, 199
MspR9I CCNGG 4 cut(s) 50, 200, 208, 341
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 3 cut(s) 50, 208, 341
MwoI GCNNNNNNNGC 7 cut(s) 59, 64, 68, 73, 268, 518, 620
NciI CCSGG 1 cut(s) 200
NdeII GATC 6 cut(s) 186, 213, 225, 287, 336, 514
NlaIII CATG 4 cut(s) 103, 478, 613, 694
NlaIV GGNNCC 4 cut(s) 121, 338, 357, 522
NmeAIII GCCGAG 4 cut(s) 88, 97, 328, 469
NmuCI GTSAC 1 cut(s) 733
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 270, 368
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 580, 665
PflMI CCANNNNNTGG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 339
PkrI GCNGC 5 cut(s) 205, 248, 460, 463, 493
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 2 cut(s) 338, 385
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 2 cut(s) 338, 385
Ppu21I YACGTR 2 cut(s) 43, 265
Psp124BI GAGCTC 2 cut(s) 141, 309
Psp6I CCWGG 3 cut(s) 48, 206, 339
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 3 cut(s) 48, 206, 339
PspN4I GGNNCC 4 cut(s) 121, 338, 357, 522
PspPI GGNCC 4 cut(s) 91, 120, 201, 244
PsuI RGATCY 2 cut(s) 336, 514
PvuI CGATCG 1 cut(s) 228
RsaI GTAC 3 cut(s) 41, 47, 284
RsaNI GTAC 3 cut(s) 40, 46, 283
SacI GAGCTC 2 cut(s) 141, 309
SatI GCNGC 5 cut(s) 204, 247, 459, 462, 492
Sau3AI GATC 6 cut(s) 186, 213, 225, 287, 336, 514
Sau96I GGNCC 4 cut(s) 91, 120, 201, 244
SchI GAGTC 2 cut(s) 338, 386
ScrFI CCNGG 4 cut(s) 50, 200, 208, 341
SduI GDGCHC 3 cut(s) 141, 264, 309
SinI GGWCC 2 cut(s) 91, 120
SsiI CCGC 6 cut(s) 204, 211, 247, 418, 428, 512
SspMI CTAG 2 cut(s) 548, 647
SstI GAGCTC 2 cut(s) 141, 309
StyD4I CCNGG 4 cut(s) 48, 198, 206, 339
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 45, 186, 267
TaqI TCGA 6 cut(s) 147, 224, 228, 290, 297, 380
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 206, 249
TfiI GAWTC 2 cut(s) 580, 665
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 3 cut(s) 458, 461, 491
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 2 cut(s) 88, 416
TspGWI ACGGA 2 cut(s) 157, 752
TspRI CASTG 1 cut(s) 570
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 91, 120
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 2 cut(s) 548, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.