AT5G66150

glycosyl hydrolase family 38 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004264
Physical Location & Seq
Reverse (-)
26438839 .. 26444434
5596 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66150.2

Sequence Viewer

Length: 3144 bp
ATGGAGAAACCGGGTATGTCTTTATTGAAAGGATCACTTTGTGTTATTGTTTTTCTTCTTCTGTTGTCGTTGGTTGAGAGCGTCAAAGGCGGGGGATACGTGAAGTATGGAACCGAAGCTAAAGTGGTTCCGGGGAAACTAAATGTTCATCTTGTTCCGCATTCGCATGACGATGTCGGCTGGTTGAAAACTGTTGATCAGTACTACGTAGGATCTAACAACAGAATCCAGAACGCTTGCGTGAGGAACGTCCTAGACTCGGTGGTGGATTCTCTACTTCGTGATCCCAATAGAAAGTTTGTGTTTGCGGAAATGGCCTTCTTTACGCGTTGGTGGGAAGAACAAAGCCCCGAGAGACAAGAGCAAGTGAGGAGACTAGTGAAATCCGGGCAACTAGAGTTTGTAAATGGAGGATGGGCTATGAATGATGAAGCAACGTGCCATTACATAGATATGATAGACCAAACCACAAAGGGGCATCGGTTTATAAAGCAACAGTTCAACACAACTCCACGTGCTGCTTGGCAAATCGATCCCTTTGGTCATTCCTCTGTTCAAGCTTACCTCCTTGGAGCAGAGTTAGGGTTGGATTCTGTGCATTTCGCGAGAATCGATTACCAAGACAGAGAAAAGCGAAAGGCCGAGAAATCACTTGAAGTCATCTGGAGAGGATCAAAAACACTGGATTCTTCTTCTCAGATATTCACCAATATCTTCTTTGTTCATTACGGCCCTCCCACTGGCTTTCACTATGAAGTCACCGACGATTACGTACCCCTCCAGGACAACCCTCGTTTCGATGGCTATAACATTAAGGAGGCGGTTAATGATTTCGTCAATGCTTCTCTTGTTTATGCTAATGTGAGTCGAGGGAACCATGTGATGTGGACAATGGGCGATGATTTTCAGTACCAGTTCGCCGAGTCTTGGTTCAGACAGATGGACAGACTTATCCATTATGTTAACAAGGATGGCCGTGTGAACGCCTTGTACTCCACACCATCTCTCTATGTTGATGCCAAGAATGTTGCCAATGTTACTTGGCCTCTCAAGACTCATGACTTTTTCCCATATGCGGATCGGGCATACGCTTACTGGACTGGCTACTTCACCAGCCGTCCTGCTCTTAAACGATATGTTCGGGCTCTTAGCGGATACTACATGGCTGCAAGGCAACTTGAATTTCTGGTGGGCAAGAACTCTGGAGGTCCAAACACTTACAGTCTTGGGGATGCTTTAGGGATAGCGCAGCACCATGATGCTGTCACTGGAACTGCCAAGCAGCATGTAACAAATGATTACATGAAACGCCTTGCTCTTGGTGCTTCTGAGGCAGAAGCTGTGGTGAATTCTGCGCTGGCATGCCTAATGAACAAAGCACCAAAAGGTGGCTGCACAAAGCCTGCAATAGCATTCAGCCAGCAGTGCTCGTTGATGAATATCAGCTATTGCCCATCGACAGAAGAAACCCTTCCAGGCCAGAAGAGCTTGATTCTGGTGGCGTATAACTCCCTTGGATGGAACCGTACAGAAATCATCAGGATTCCGGTCAATGATGCAGGTCTAAGTGTGGAAGATTCATCTGGAAACACCCTTGATGCTCAATACATCCCCATGGATAATGTCACGAGCAACCTCAGAAGTTTCTACACAAAAGCTTATTTAGGAATATCTTCCCTGCAGCGTCCAAAGTATTGGCTCGTGTTTAAAGCAAAGGTGCCACCACTTGGTTGGAACACATTCTTCATATCTAAAGCATCCGCACAAGGAAGCAACAATCACAAACACTCTTCAGTGATGCTTAGTCCGATGAACAATACGACTGAAATTGGTCCAGGAAATCTAAAGATGGTGTTTTCTTCAGACTCTGGTCGTCTCGAACGGATGTACAACTCCAGAACAGGAGCTGACATAAAGGTGGATCAGAACTATTTCTGGTATGCTTCAAATGTTGGGGACGCAAAAGATCCCCAGGTTTCTGGTGCATATATATTTCGACCTAATGGTTCCTTGGCGTATCCTGTTTCGTCGTCCAAAATATGTACCGTGACTAGTGCTTTCATTGGCAATGGAAACGTGCAGTCAAAGTTGCAGATTGTGCGAGGGCCTTTAATAGATGAGGTGCACCAGCAGTTTAGTCCTTGGGTGGCACAGGTGGTAAGACTATACAAAGAAAAAGAGCACGCCGAATTTGAGTTCACTATTGGTCCAATTTCTGTTGGTAAGGGACATCTAACAGGAAAAGAGATTATCACGCGAATGGTAACAGATATGACTACAGCTAAGGAGTTCTATACTGACTCTAATGGAAGAGACTTTCTGAAACGGGTCCGGGACAACCGAACAGACTGGCATCTCGAAGTGAATGAGCCAATAGCAGGAAACTACTATCCGCTTAATCTTGGAATGTACATAAAAGACGAGAAAGCAGAGTTGTCGGTGTTGGTTGATCGGGCTACTGGGGGTGCAAGTATTAAGGACGGTGAAATAGAGTTGATGCTTCACAGGAGAACGTCCATGGATGACTCGAGAGGAGTGGAGGAGAGCCTTGTAGAAACTGTGTGTGTGAATGACACCTGCGCAGGATTGACGATTCGTGGGAATTACTATGTAAGCATAAATAAGGTAGGAGAAGGAGGGCGGTGGAGACGAGAGACTGGACAGGAAATATACTCACCTCTTCTAATGGCATTCGCACACGAGAACAAGGAGAAATGGAAAGCCTCAAATACTGTGAAAGGTTATGCCATGGATCATCTCTACACCTTGCCTCAAAACATTGCTCTCATCACTCTTGAGGAGTTGGATCTCGGGAACGTCCTTCTTCGCCTGGCTCATCTCTACGAGGCGGGAGAAGACAGTGATTACTCTAAAATCGCTAAAGTTGAGTTGAAGAAGTTGTTTTCCGGCAAAATGATCAAAGAAGTGACGGAAATGAGTTTGTCAGCAAATCAAGAGAAAGTAAAGATGAAGGAGAAGATGAAGTGGAAAGTGGAGGGTGAAGCGGAACAACCTTCTTCCCCGCTAAGAGGCGGACCCGTTGACAAGTCGACTTTAGTAGTAGAGCTTGGTCCCATGGAGATTCGTACATTTGTGGTTCAGTTTTATCAGAAACAACGAAGAAGAAAGTTGTTTGTGGGATGA

Protein Analysis

1047

Amino Acids

118.01

Weight (kDa)

8.33

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 48 - 359 6.5e-83 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 364 - 456 1.8e-21 Alpha mannosidase middle domain
Laman-like_dom PF21260 559 - 582 8.5e-07 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 610 - 843 9.7e-48 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 925 - 1030 1.4e-07 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 488
AarI CACCTGC 1 cut(s) 2585
Acc16I TGCGCA 1 cut(s) 2581
Acc36I ACCTGC 2 cut(s) 1550, 2585
AccB1I GGYRCC 1 cut(s) 1717
AccB7I CCANNNNNTGG 2 cut(s) 1388, 1727
AccI GTMKAC 1 cut(s) 3050
AccII CGCG 3 cut(s) 328, 605, 2257
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 3 cut(s) 1181, 1348, 2189
AcuI CTGAAG 2 cut(s) 1776, 1845
AcvI CACGTG 1 cut(s) 515
AdeI CACNNNGTG 1 cut(s) 41
AfaI GTAC 9 cut(s) 203, 774, 911, 992, 1528, 1889, 2044, 2411, 3088
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 8 cut(s) 28, 187, 502, 557, 656, 1181, 1947, 2893
AhlI ACTAGT 2 cut(s) 376, 2051
AjnI CCWGG 5 cut(s) 780, 1474, 1834, 1971, 2829
AluBI AGCT 9 cut(s) 119, 560, 1340, 1446, 1488, 1658, 1907, 2282, 3067
AluI AGCT 9 cut(s) 119, 560, 1340, 1446, 1488, 1658, 1907, 2282, 3067
Alw21I GWGCWC 3 cut(s) 1430, 2127, 2184
Alw26I GTCTC 6 cut(s) 349, 367, 1880, 2307, 2641, 2648
Alw44I GTGCAC 1 cut(s) 2123
AlwNI CAGNNNCTG 3 cut(s) 1340, 1868, 1907
Ama87I CYCGRG 3 cut(s) 350, 2527, 2810
AoxI GGCC 7 cut(s) 315, 639, 730, 973, 1043, 1477, 2105
ApaLI GTGCAC 1 cut(s) 2123
ApeKI GCWGC 6 cut(s) 518, 1166, 1249, 1282, 1392, 1681
ApoI RAATTY 3 cut(s) 1181, 1348, 2189
Asp700I GAANNNNTTC 4 cut(s) 1470, 1672, 1739, 1931
AspLEI GCGC 3 cut(s) 1249, 1357, 2582
AspS9I GGNCC 8 cut(s) 731, 1208, 1832, 2105, 2207, 2329, 3035, 3071
AsuC2I CCSGG 4 cut(s) 12, 132, 388, 2333
AsuHPI GGTGA 7 cut(s) 697, 751, 1102, 1357, 2495, 2667, 3011
AvaI CYCGRG 3 cut(s) 350, 2527, 2810
AvaII GGWCC 6 cut(s) 1208, 1832, 2207, 2329, 3035, 3071
BaeGI GKGCMC 1 cut(s) 2127
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 1 cut(s) 1147
BauI CACGAG 3 cut(s) 1627, 1700, 2699
BbrPI CACGTG 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 2862
Bbv12I GWGCWC 3 cut(s) 1430, 2127, 2184
BbvI GCAGC 6 cut(s) 505, 1153, 1261, 1294, 1379, 1693
BccI CCATC 8 cut(s) 408, 794, 934, 965, 1009, 1462, 1512, 1843
BceAI ACGGC 3 cut(s) 745, 960, 1101
BcgI CGANNNNNNTGC 2 cut(s) 2418, 2452
BciT130I CCWGG 5 cut(s) 782, 1476, 1836, 1973, 2831
BciVI GTATCC 3 cut(s) 89, 1148, 2028
BclI TGATCA 2 cut(s) 196, 2916
BcnI CCSGG 4 cut(s) 12, 132, 388, 2333
BcoDI GTCTC 6 cut(s) 349, 367, 1880, 2307, 2641, 2648
BcuI ACTAGT 2 cut(s) 376, 2051
BfaI CTAG 4 cut(s) 254, 377, 395, 2052
BfmI CTRYAG 2 cut(s) 1679, 2277
BfuAI ACCTGC 2 cut(s) 1550, 2585
BfuI GTATCC 3 cut(s) 89, 1148, 2028
BisI GCNGC 6 cut(s) 519, 1167, 1250, 1283, 1393, 1682
BlsI GCNGC 6 cut(s) 520, 1168, 1251, 1284, 1394, 1683
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 9 cut(s) 12, 132, 388, 782, 1476, 1836, 1973, 2333, 2831
Bme18I GGWCC 6 cut(s) 1208, 1832, 2207, 2329, 3035, 3071
BmeT110I CYCGRG 3 cut(s) 350, 2527, 2810
BmgT120I GGNCC 8 cut(s) 731, 1208, 1832, 2105, 2207, 2329, 3035, 3071
BmiI GGNNCC 9 cut(s) 112, 129, 875, 1523, 1719, 2008, 2330, 3037, 3073
BmrFI CCNGG 9 cut(s) 12, 132, 388, 782, 1476, 1836, 1973, 2333, 2831
BmrI ACTGGG 1 cut(s) 2469
BmuI ACTGGG 1 cut(s) 2469
BoxI GACNNNNGTC 1 cut(s) 1869
BpiI GAAGAC 1 cut(s) 2862
BpmI CTGGAG 4 cut(s) 685, 764, 1224, 1879
Bpu10I CCTNAGC 1 cut(s) 2283
BpuEI CTTGAG 2 cut(s) 1034, 2816
BpuMI CCSGG 4 cut(s) 12, 132, 388, 2333
Bsa29I ATCGAT 2 cut(s) 531, 612
BsaAI YACGTR 4 cut(s) 100, 208, 515, 772
BsaBI GATNNNNATC 1 cut(s) 1439
BsaWI WCCGGW 1 cut(s) 1546
Bse1I ACTGG 9 cut(s) 687, 745, 913, 1100, 1105, 1273, 2354, 2464, 2662
Bse3DI GCAATG 2 cut(s) 2074, 2778
Bse8I GATNNNNATC 1 cut(s) 1439
BseBI CCWGG 5 cut(s) 782, 1476, 1836, 1973, 2831
BseCI ATCGAT 2 cut(s) 531, 612
BseGI GGATG 8 cut(s) 419, 976, 1237, 1523, 1608, 1757, 1890, 2527
BseJI GATNNNNATC 1 cut(s) 1439
BseMI GCAATG 2 cut(s) 2074, 2778
BseMII CTCAG 3 cut(s) 710, 1320, 1651
BseNI ACTGG 9 cut(s) 687, 745, 913, 1100, 1105, 1273, 2354, 2464, 2662
BseRI GAGGAG 4 cut(s) 385, 2547, 2555, 2813
BseSI GKGCMC 1 cut(s) 2127
BseXI GCAGC 6 cut(s) 505, 1153, 1261, 1294, 1379, 1693
BsgI GTGCAG 2 cut(s) 1378, 2099
Bsh1236I CGCG 3 cut(s) 328, 605, 2257
BshFI GGCC 7 cut(s) 317, 641, 732, 975, 1045, 1479, 2107
BshNI GGYRCC 1 cut(s) 1717
BshVI ATCGAT 2 cut(s) 531, 612
BsiHKAI GWGCWC 3 cut(s) 1430, 2127, 2184
BsiHKCI CYCGRG 3 cut(s) 350, 2527, 2810
BsiSI CCGG 6 cut(s) 11, 131, 387, 1547, 2332, 2907
BslFI GGGAC 4 cut(s) 1970, 2241, 2348, 3057
BsmAI GTCTC 6 cut(s) 349, 367, 1880, 2307, 2641, 2648
BsmBI CGTCTC 2 cut(s) 1880, 2641
BsmFI GGGAC 4 cut(s) 1970, 2241, 2348, 3057
BsmI GAATGC 3 cut(s) 160, 1412, 2690
BsnI GGCC 7 cut(s) 317, 641, 732, 975, 1045, 1479, 2107
BsoBI CYCGRG 3 cut(s) 350, 2527, 2810
Bsp1286I GDGCHC 4 cut(s) 1147, 1430, 2127, 2184
Bsp1407I TGTACA 2 cut(s) 1887, 2409
Bsp19I CCATGG 4 cut(s) 1614, 2517, 2748, 3075
Bsp68I TCGCGA 1 cut(s) 605
BspANI GGCC 7 cut(s) 317, 641, 732, 975, 1045, 1479, 2107
BspCNI CTCAG 3 cut(s) 709, 1321, 1650
BspDI ATCGAT 2 cut(s) 531, 612
BspFNI CGCG 3 cut(s) 328, 605, 2257
BspHI TCATGA 1 cut(s) 1057
BspLI GGNNCC 9 cut(s) 112, 129, 875, 1523, 1719, 2008, 2330, 3037, 3073
BspMAI CTGCAG 1 cut(s) 1683
BspMI ACCTGC 2 cut(s) 1550, 2585
BspQI GCTCTTC 1 cut(s) 1478
BspT107I GGYRCC 1 cut(s) 1717
BsrDI GCAATG 2 cut(s) 2074, 2778
BsrGI TGTACA 2 cut(s) 1887, 2409
BsrI ACTGG 9 cut(s) 687, 745, 913, 1100, 1105, 1273, 2354, 2464, 2662
BssSI CACGAG 3 cut(s) 1627, 1700, 2699
BssT1I CCWWGG 8 cut(s) 568, 1513, 1614, 2010, 2141, 2517, 2748, 3075
Bst2BI CACGAG 3 cut(s) 1627, 1700, 2699
Bst2UI CCWGG 5 cut(s) 782, 1476, 1836, 1973, 2831
Bst4CI ACNGT 9 cut(s) 193, 498, 1223, 1526, 2047, 2483, 2560, 2734, 2861
Bst6I CTCTTC 4 cut(s) 1478, 1795, 2305, 2685
BstAPI GCANNNNNTGC 1 cut(s) 2098
BstAUI TGTACA 2 cut(s) 1887, 2409
BstBAI YACGTR 4 cut(s) 100, 208, 515, 772
BstC8I GCNNGC 6 cut(s) 238, 1359, 1363, 1404, 1421, 2184
BstDEI CTNAG 8 cut(s) 696, 1148, 1329, 1565, 1637, 1802, 2283, 3026
BstDSI CCRYGG 4 cut(s) 1614, 2517, 2748, 3075
BstF5I GGATG 8 cut(s) 419, 976, 1237, 1523, 1608, 1757, 1890, 2527
BstFNI CGCG 3 cut(s) 328, 605, 2257
BstHHI GCGC 3 cut(s) 1249, 1357, 2582
BstMAI GTCTC 6 cut(s) 349, 367, 1880, 2307, 2641, 2648
BstMWI GCNNNNNNNGC 8 cut(s) 87, 314, 1082, 1322, 1331, 1425, 1485, 2098
BstNI CCWGG 5 cut(s) 782, 1476, 1836, 1973, 2831
BstNSI RCATGY 2 cut(s) 1289, 1365
BstPAI GACNNNNGTC 1 cut(s) 1869
BstSCI CCNGG 9 cut(s) 10, 130, 386, 780, 1474, 1834, 1971, 2331, 2829
BstSFI CTRYAG 2 cut(s) 1679, 2277
BstSLI GKGCMC 1 cut(s) 2127
BstSNI TACGTA 2 cut(s) 208, 772
BstUI CGCG 3 cut(s) 328, 605, 2257
BstV1I GCAGC 6 cut(s) 505, 1153, 1261, 1294, 1379, 1693
BstV2I GAAGAC 1 cut(s) 2862
BstX2I RGATCY 3 cut(s) 212, 1966, 2806
BstXI CCANNNNNNTGG 3 cut(s) 1695, 1731, 1979
BstYI RGATCY 3 cut(s) 212, 1966, 2806
Bsu15I ATCGAT 2 cut(s) 531, 612
BsuI GTATCC 3 cut(s) 89, 1148, 2028
BsuRI GGCC 7 cut(s) 317, 641, 732, 975, 1045, 1479, 2107
BsuTUI ATCGAT 2 cut(s) 531, 612
BtgI CCRYGG 4 cut(s) 1614, 2517, 2748, 3075
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 8 cut(s) 419, 976, 1237, 1523, 1608, 1757, 1890, 2527
BtsI GCAGTG 1 cut(s) 1430
BtsIMutI CAGTG 6 cut(s) 680, 738, 1266, 1430, 1800, 2866
BtuMI TCGCGA 1 cut(s) 605
BveI ACCTGC 2 cut(s) 1550, 2585
Cac8I GCNNGC 6 cut(s) 238, 1359, 1363, 1404, 1421, 2184
CaiI CAGNNNCTG 3 cut(s) 1340, 1868, 1907
CciI TCATGA 1 cut(s) 1057
CfoI GCGC 3 cut(s) 1249, 1357, 2582
Cfr13I GGNCC 8 cut(s) 731, 1208, 1832, 2105, 2207, 2329, 3035, 3071
ClaI ATCGAT 2 cut(s) 531, 612
CseI GACGC 3 cut(s) 70, 1673, 1967
Csp6I GTAC 9 cut(s) 202, 773, 910, 991, 1527, 1888, 2043, 2410, 3087
CviQI GTAC 9 cut(s) 202, 773, 910, 991, 1527, 1888, 2043, 2410, 3087
DdeI CTNAG 8 cut(s) 696, 1148, 1329, 1565, 1637, 1802, 2283, 3026
DraI TTTAAA 1 cut(s) 1708
DraIII CACNNNGTG 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 4 cut(s) 1478, 1795, 2305, 2685
EarI CTCTTC 4 cut(s) 1478, 1795, 2305, 2685
EciI GGCGGA 1 cut(s) 3048
Eco105I TACGTA 2 cut(s) 208, 772
Eco130I CCWWGG 8 cut(s) 568, 1513, 1614, 2010, 2141, 2517, 2748, 3075
Eco24I GRGCYC 1 cut(s) 1147
Eco47I GGWCC 6 cut(s) 1208, 1832, 2207, 2329, 3035, 3071
Eco57I CTGAAG 2 cut(s) 1776, 1845
Eco72I CACGTG 1 cut(s) 515
Eco88I CYCGRG 3 cut(s) 350, 2527, 2810
EcoO109I RGGNCCY 1 cut(s) 2105
EcoRI GAATTC 1 cut(s) 1348
EcoRII CCWGG 5 cut(s) 780, 1474, 1834, 1971, 2829
EcoT14I CCWWGG 8 cut(s) 568, 1513, 1614, 2010, 2141, 2517, 2748, 3075
EcoT38I GRGCYC 1 cut(s) 1147
ErhI CCWWGG 8 cut(s) 568, 1513, 1614, 2010, 2141, 2517, 2748, 3075
Esp3I CGTCTC 2 cut(s) 1880, 2641
FalI AAGNNNNNCTT 4 cut(s) 21, 53, 1455, 1487
FaqI GGGAC 4 cut(s) 1970, 2241, 2348, 3057
FauI CCCGC 3 cut(s) 83, 2842, 3030
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 2 cut(s) 196, 2916
FblI GTMKAC 1 cut(s) 3050
Fnu4HI GCNGC 6 cut(s) 519, 1167, 1250, 1283, 1393, 1682
FokI GGATG 8 cut(s) 426, 983, 1244, 1530, 1595, 1744, 1897, 2534
FriOI GRGCYC 1 cut(s) 1147
Fsp4HI GCNGC 6 cut(s) 519, 1167, 1250, 1283, 1393, 1682
FspBI CTAG 4 cut(s) 254, 377, 395, 2052
FspI TGCGCA 1 cut(s) 2581
GlaI GCGC 3 cut(s) 1248, 1356, 2581
GluI GCNGC 6 cut(s) 519, 1167, 1250, 1283, 1393, 1682
GsuI CTGGAG 4 cut(s) 685, 764, 1224, 1879
HaeIII GGCC 7 cut(s) 317, 641, 732, 975, 1045, 1479, 2107
HapII CCGG 6 cut(s) 11, 131, 387, 1547, 2332, 2907
HgaI GACGC 3 cut(s) 70, 1673, 1967
HhaI GCGC 3 cut(s) 1249, 1357, 2582
Hin6I GCGC 3 cut(s) 1247, 1355, 2580
HinP1I GCGC 3 cut(s) 1247, 1355, 2580
HincII GTYRAC 3 cut(s) 964, 3043, 3051
HindII GTYRAC 3 cut(s) 964, 3043, 3051
HindIII AAGCTT 2 cut(s) 558, 1656
HpaI GTTAAC 1 cut(s) 964
HpaII CCGG 6 cut(s) 11, 131, 387, 1547, 2332, 2907
HphI GGTGA 7 cut(s) 697, 751, 1102, 1357, 2495, 2667, 3011
Hpy166II GTNNAC 7 cut(s) 888, 964, 982, 2125, 2199, 3043, 3051
Hpy188I TCNGA 9 cut(s) 699, 935, 1330, 1640, 1809, 1864, 1926, 2322, 3111
Hpy8I GTNNAC 7 cut(s) 888, 964, 982, 2125, 2199, 3043, 3051
Hpy99I CGWCG 2 cut(s) 767, 2032
HpyAV CCTTC 6 cut(s) 328, 1481, 2627, 2831, 2965, 3024
HpyCH4III ACNGT 9 cut(s) 193, 498, 1223, 1526, 2047, 2483, 2560, 2734, 2861
HpyCH4IV ACGT 9 cut(s) 99, 207, 249, 437, 514, 771, 2076, 2513, 2817
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 314, 1082, 1322, 1331, 1425, 1485, 2098
HpyF3I CTNAG 8 cut(s) 696, 1148, 1329, 1565, 1637, 1802, 2283, 3026
HpySE526I ACGT 9 cut(s) 99, 207, 249, 437, 514, 771, 2076, 2513, 2817
HspAI GCGC 3 cut(s) 1247, 1355, 2580
Ksp22I TGATCA 2 cut(s) 196, 2916
KspAI GTTAAC 1 cut(s) 964
LguI GCTCTTC 1 cut(s) 1478
LmnI GCTCC 2 cut(s) 572, 1904
Lsp1109I GCAGC 6 cut(s) 505, 1153, 1261, 1294, 1379, 1693
MaeI CTAG 4 cut(s) 254, 377, 395, 2052
MaeII ACGT 9 cut(s) 99, 207, 249, 437, 514, 771, 2076, 2513, 2817
MaeIII GTNAC 8 cut(s) 757, 1036, 1264, 1288, 1624, 2047, 2263, 2926
MflI RGATCY 3 cut(s) 212, 1966, 2806
MhlI GDGCHC 4 cut(s) 1147, 1430, 2127, 2184
MluCI AATT 6 cut(s) 1181, 1348, 1827, 2189, 2211, 2602
MluI ACGCGT 1 cut(s) 326
MlyI GAGTC 7 cut(s) 251, 874, 932, 1048, 1859, 2294, 2519
MmeI TCCRAC 3 cut(s) 567, 1712, 2784
MroXI GAANNNNTTC 4 cut(s) 1470, 1672, 1739, 1931
MseI TTAA 8 cut(s) 813, 825, 963, 1128, 1707, 2111, 2397, 2475
MslI CAYNNNNRTG 7 cut(s) 165, 171, 452, 1257, 1613, 1916, 2258
MspI CCGG 6 cut(s) 11, 131, 387, 1547, 2332, 2907
MspR9I CCNGG 9 cut(s) 12, 132, 388, 782, 1476, 1836, 1973, 2333, 2831
Mva1269I GAATGC 3 cut(s) 160, 1412, 2690
MvaI CCWGG 5 cut(s) 782, 1476, 1836, 1973, 2831
MvnI CGCG 3 cut(s) 328, 605, 2257
MwoI GCNNNNNNNGC 8 cut(s) 87, 314, 1082, 1322, 1331, 1425, 1485, 2098
NciI CCSGG 4 cut(s) 12, 132, 388, 2333
NcoI CCATGG 4 cut(s) 1614, 2517, 2748, 3075
NdeI CATATG 1 cut(s) 1072
NlaIV GGNNCC 9 cut(s) 112, 129, 875, 1523, 1719, 2008, 2330, 3037, 3073
NmeAIII GCCGAG 2 cut(s) 667, 946
NmuCI GTSAC 5 cut(s) 757, 1264, 1624, 2047, 2926
NruI TCGCGA 1 cut(s) 605
NsbI TGCGCA 1 cut(s) 2581
NspI RCATGY 2 cut(s) 1289, 1365
PaeI GCATGC 1 cut(s) 1365
PaeR7I CTCGAG 1 cut(s) 2527
PagI TCATGA 1 cut(s) 1057
PaqCI CACCTGC 1 cut(s) 2585
PciSI GCTCTTC 1 cut(s) 1478
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PctI GAATGC 3 cut(s) 160, 1412, 2690
PdmI GAANNNNTTC 4 cut(s) 1470, 1672, 1739, 1931
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 2 cut(s) 1388, 1727
PfoI TCCNGGA 3 cut(s) 780, 1834, 2331
PkrI GCNGC 6 cut(s) 520, 1168, 1251, 1284, 1394, 1683
PleI GAGTC 7 cut(s) 251, 873, 931, 1048, 1859, 2294, 2519
PmaCI CACGTG 1 cut(s) 515
PmlI CACGTG 1 cut(s) 515
PpsI GAGTC 7 cut(s) 251, 873, 931, 1048, 1859, 2294, 2519
Ppu21I YACGTR 4 cut(s) 100, 208, 515, 772
PshAI GACNNNNGTC 1 cut(s) 1869
PsiI TTATAA 1 cut(s) 488
Psp6I CCWGG 5 cut(s) 780, 1474, 1834, 1971, 2829
PspCI CACGTG 1 cut(s) 515
PspGI CCWGG 5 cut(s) 780, 1474, 1834, 1971, 2829
PspN4I GGNNCC 9 cut(s) 112, 129, 875, 1523, 1719, 2008, 2330, 3037, 3073
PspPI GGNCC 8 cut(s) 731, 1208, 1832, 2105, 2207, 2329, 3035, 3071
PsrI GAACNNNNNNTAC 2 cut(s) 974, 1006
PstI CTGCAG 1 cut(s) 1683
PstNI CAGNNNCTG 3 cut(s) 1340, 1868, 1907
PsuI RGATCY 3 cut(s) 212, 1966, 2806
PsyI GACNNNGTC 1 cut(s) 173
RruI TCGCGA 1 cut(s) 605
RsaI GTAC 9 cut(s) 203, 774, 911, 992, 1528, 1889, 2044, 2411, 3088
RsaNI GTAC 9 cut(s) 202, 773, 910, 991, 1527, 1888, 2043, 2410, 3087
RseI CAYNNNNRTG 7 cut(s) 165, 171, 452, 1257, 1613, 1916, 2258
SalI GTCGAC 1 cut(s) 3049
SapI GCTCTTC 1 cut(s) 1478
SaqAI TTAA 8 cut(s) 813, 825, 963, 1128, 1707, 2111, 2397, 2475
SatI GCNGC 6 cut(s) 519, 1167, 1250, 1283, 1393, 1682
Sau96I GGNCC 8 cut(s) 731, 1208, 1832, 2105, 2207, 2329, 3035, 3071
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 7 cut(s) 251, 874, 932, 1048, 1859, 2294, 2519
ScrFI CCNGG 9 cut(s) 12, 132, 388, 782, 1476, 1836, 1973, 2333, 2831
SduI GDGCHC 4 cut(s) 1147, 1430, 2127, 2184
SfcI CTRYAG 2 cut(s) 1679, 2277
Sfr274I CTCGAG 1 cut(s) 2527
SinI GGWCC 6 cut(s) 1208, 1832, 2207, 2329, 3035, 3071
SlaI CTCGAG 1 cut(s) 2527
SmiMI CAYNNNNRTG 7 cut(s) 165, 171, 452, 1257, 1613, 1916, 2258
SmlI CTYRAG 3 cut(s) 1049, 2527, 2795
SmoI CTYRAG 3 cut(s) 1049, 2527, 2795
SnaBI TACGTA 2 cut(s) 208, 772
SpeI ACTAGT 2 cut(s) 376, 2051
SphI GCATGC 1 cut(s) 1365
Sse9I AATT 6 cut(s) 1181, 1348, 1827, 2189, 2211, 2602
SspMI CTAG 4 cut(s) 254, 377, 395, 2052
StyD4I CCNGG 9 cut(s) 10, 130, 386, 780, 1474, 1834, 1971, 2331, 2829
StyI CCWWGG 8 cut(s) 568, 1513, 1614, 2010, 2141, 2517, 2748, 3075
TaaI ACNGT 9 cut(s) 193, 498, 1223, 1526, 2047, 2483, 2560, 2734, 2861
TaiI ACGT 9 cut(s) 102, 210, 252, 440, 517, 774, 2079, 2516, 2820
TasI AATT 6 cut(s) 1181, 1348, 1827, 2189, 2211, 2602
TatI WGTACW 4 cut(s) 201, 990, 1887, 2409
Tru1I TTAA 8 cut(s) 813, 825, 963, 1128, 1707, 2111, 2397, 2475
Tru9I TTAA 8 cut(s) 813, 825, 963, 1128, 1707, 2111, 2397, 2475
TscAI CASTG 6 cut(s) 687, 745, 1273, 1430, 1800, 2866
TseFI GTSAC 5 cut(s) 757, 1264, 1624, 2047, 2926
TseI GCWGC 6 cut(s) 518, 1166, 1249, 1282, 1392, 1681
Tsp45I GTSAC 5 cut(s) 757, 1264, 1624, 2047, 2926
TspGWI ACGGA 2 cut(s) 1897, 2945
TspRI CASTG 6 cut(s) 687, 745, 1273, 1430, 1800, 2866
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 1388, 1727
VneI GTGCAC 1 cut(s) 2123
VpaK11BI GGWCC 6 cut(s) 1208, 1832, 2207, 2329, 3035, 3071
XapI RAATTY 3 cut(s) 1181, 1348, 2189
XceI RCATGY 2 cut(s) 1289, 1365
XcmI CCANNNNNNNNNTGG 2 cut(s) 519, 1728
XhoI CTCGAG 1 cut(s) 2527
XmiI GTMKAC 1 cut(s) 3050
XmnI GAANNNNTTC 4 cut(s) 1470, 1672, 1739, 1931
XspI CTAG 4 cut(s) 254, 377, 395, 2052
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.