Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004264

Physical Location & Seq

Genomic Coordinates Reverse (-)

10434313 .. 10440653

Gene Structure 6341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGGGAAAGGCGGAAACTCTGGGGTTGGGTTTTCGGCTTGTGGTTGTTTTGGTTGTGGGTTGTTTGTATAGGGAGGCTGTTGGCAGCGGTTTTGTGAAGTATGAGACGGGGAGTGGCGTGGTGGAGGGGAAACTGAATGTTCATTTGGTGGCGCATTCACACGATGATGTCGGGTGGTTGAAGACGGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTTGTTGAATCGTTGGTCCGTGATCCGAACAGGAAGTTTGTGTTTGCTGAGATGGCATTCTTCCAAAGATGGTGGTTAACACAAAGTCCGGGAATTCAAGCTCTCGTGCGAAAACTAGTAGATGCCGGGCAGTTAGAGTTTGTAAATGGAGGTTGGTGTATGCATGATGAAGCAACAACCCATTACATAGACATGATTGATCAAACAACGCTAGGTCACCGTGCAATAAAGGCGCAGTTCAACAAGACGCCTCGTGCTGGATGGCAGATTGATCCTTTTGGACACTCTGCCGTGCAAGCTTACTTACTCACAGCCGAGCTCGGGTTTGATTCTGTGCATTTTGCAAGGATTGATTATCAGGACAGAGCGAAGCGCAAGGAGGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTCGGTTCTTCATCTCAGATTTTTGCCAATGCTTTTCCGGTTAACTATAATCCACCTCCCGGTTTCCATTTCGAAGTATTTGACGACGTAGTCCCTGTCCAGGATAATCCTCTTCTGTTCGACTACAATGTTGAACAGCGTGTAAGCGATTTCATCAGCGCTTCAGTTACCCAAGCAAATGTGACAAGGACGAATCACGTTATGTGGACGATGGGTGATGATTTCCAGTACCAATATGCAGAATCATGGTTCAAACAAATGGACAAGTTAATTCACTATGTTAATAAGGACGGTCGAGTTAATGCCTTGTATTCTACACCATCTATCTATACCGATGCTAAAAATGCAGCAAACCAGTCATGGCCACTGAAAACTGAGGATTATTTTCCGTATGCAGATGCGGTAGATGCTTATTGGACTGGATTTTTCACTAGTCGCCCGGGTTTGAAGGGATATATCCGCTTGCTAAGCGGATATTATTTGGCGGCACGAGAACTAGAATTTCTGGCTGGAAGGAGAGCAAATGGTTCCAATACAGATGGCCTTGCAGATGCTTTAGGAACCGCACAGCACCATGATGCAGTCACCGGCACTGCCTTACAACATACTACAAATGACTACGCAAAGCGCCTTTTCATCGGAGCTTCTGAGGCAGAAGCTGTTGTCAGTTCCGCTCTTTTGTGTCTAACTACAAATAAAACAGGAGATCAATGTACTAGCTCAACACCGACTTTTAGCCAGTGTCAATCACTCAATATCAGTTACTGCCCTCCAACAGAGGAAGATATTCCACAAGGGAAGAGCTTGGTTGTTGTGGCGTATAATCCTCTTGGATGGAATCGGGCTGAGATTGTTCGGATTCCTGTTAATGATGCAACTCTTTCTGTTCAAGATTCTTCGGGAAATATTCTTGAGGTACAATATGTAAGTTTGGACAATGTAACAGCGAATTTAAGAAACTTCTACGTAAAAGCCTACTTGGGACAGCCACCAGACCAAGTTCCAAAGTTCTGGCTAATCTTTCAAGTCTCCGTGCAACCACTTGGTTGGAACACATACTTCATTTCAAGAAGAACTACCAAACGTAGATTGAAGAGCGGAAATGGATTTCTTTCTGTGATGGACAGTCCACAGAATGGAACAATTGAAATCGGACCTGGAGATCTGAAAATGTCGTTTTCTTTAGCTTCGGGACAACTCAATCGGATGTATAATTCTAAAACAGGAGTTGATGTACCGATACAGCAGAGCTACCTCTGGTATGGTTCAAGCAGAGGCGATGCCGATTCCCAGCAAGCTTCTGGTGCATACGTATTCCGGCCTAATGGCGCCCCTCCAACTGTTGTGTCAAGATCAGTTCCTTTAAAGGTCTTTCGTGGACCGATAGTTGATGAGATTCACCAACAGTTCAGTCCATGGATCTATCAGGTCACTAGACTCTACAGAGACAAAGAGCATGCCGAAGTTGAATATACGATCGGTCCTATTCCCACAGAAGACAGTGTGGGAAAGGAGGTCATCACAAGAATCACAGCAAACATGGAAACAAACAAGGAGTTCTACACCGATTCTAACGGAAGGGACTTCATTAAACGGGTGCGAGATTATAGACCAGATTGGCCCCTTGTGGTTAATCAACCTGTGGCAGGAAATTATTATCCACTAAGTCTTGGAATGTTTACTTCGGATAACAAATCCGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCAAGCATTGAAGATGGACAAGTAGAGCTGATGCTTCACAGGCGTCTACTTGAGGACGATCCAAGTGGACTGGGTGAAGCTCTTGATGAAATAGTGTGCATCGAAGAAAATACATGCGAAGGACTAACGGTTCGAGGGAACTATTATATGAGCGTAAACCAGGCAGGGTCGGGTGCCCCCTGGCGCCGCACAACTGGCCAGGAAGTTTACTCTCCGCTTCTTCTAGCATTCGCCCACGAGAAAATGGAGGATTGGACTGCATCACATTTGACAAAATCATCTATCATGGATTCTAATTACAGCTTGCCTCTCAATGTTGCCTTAATTACTCTTCAGGAACTGGATGACGGTAGTGTGCTCCTTCGGTTAGCTCATCTATATGAGGCAGACGAAGATCCTCAATATTCAACACTGGCCAAAGTTGAACTGAAGAAGATGCTTACAAGAAAAGCGATAAGGGAGGTGAAGGAAATGAGCTTGTCTGCAAATCAAGAGAAGTCGGAGATGAAGAAGATGACATGGAAAGTCGAGGGTGACGGTGGAGATGTACCTACACCGGTTAGAGGGGGGCCCGTCGATGGCTCAACTCTTGTTGTTGAGCTTGGACCCATGGAGATACGCACCTTTGTATTGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009987 GO:0015923 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1016

Amino Acids

113.33

Weight (kDa)

5.41

Isoelectric Point (pI)

38.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	46 - 357	2.4e-80	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	362 - 452	1.8e-16	Alpha mannosidase middle domain
Glyco_hydro_38C	PF07748	612 - 825	1.1e-44	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	908 - 1012	3.8e-09	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66150 AT5G66150 AT5G66150
fragaria_vesca	FvH4_1g11550 FvH4_1g11600 FvH4_1g11600 FvH4_1g11600
malus_domestica	MD02G1128700.v1.1 MD02G1129000.v1.1
prunus_persica	Prupe.7G170500_v2.0.a1 Prupe.7G170500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0099191 RchiOBHm_Chr2g0099201
rosa_laevigata	RLG00000016854 RLG00000016860
rosa_multiflora	Rmu_co8341649.1_g000001 Rmu_sc0002025.1_g000008 Rmu_sc0010993.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143340 Rroxscaffold_2G00143370
rosa_rugosa	Rorug02G0077600 Rorug02G0078100 Rorug02G0078100
rosa_samantha	Rh2AG126500 Rh2AG126900 Rh2BG129700 Rh2BG129900 Rh2CG130700 Rh2CG131100 Rh2DG131100 Rh2DG131500
rosa_wichuraiana	Rw2G009780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	764
AccB1I	GGYRCC	3 cut(s)	2003, 2582, 2592
AccB7I	CCANNNNNTGG	1 cut(s)	1811
AccBSI	CCGCTC	2 cut(s)	1349, 1773
AccI	GTMKAC	1 cut(s)	2455
AclWI	GGATC	9 cut(s)	214, 272, 521, 660, 673, 2102, 2416, 2462, 2798
AcoI	YGGCCR	3 cut(s)	1037, 2605, 2823
AcsI	RAATTY	3 cut(s)	348, 1175, 1624
AcuI	CTGAAG	3 cut(s)	822, 2726, 2858
AcyI	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
AfaI	GTAC	6 cut(s)	197, 905, 1390, 1593, 1911, 2958
AfeI	AGCGCT	1 cut(s)	835
AfiI	CCNNNNNNNGG	8 cut(s)	512, 576, 734, 775, 1811, 2321, 2972, 2987
AgeI	ACCGGT	1 cut(s)	2965
AhlI	ACTAGT	2 cut(s)	370, 1106
AjnI	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
AjuI	GAANNNNNNNTTGG	2 cut(s)	128, 160
AloI	GAACNNNNNNTCC	2 cut(s)	2523, 2555
Alw21I	GWGCWC	2 cut(s)	576, 2769
Alw26I	GTCTC	4 cut(s)	98, 648, 1708, 2115
AlwI	GGATC	9 cut(s)	214, 272, 521, 660, 673, 2102, 2416, 2462, 2798
AlwNI	CAGNNNCTG	3 cut(s)	1334, 1440, 2749
Ama87I	CYCGRG	2 cut(s)	575, 1114
Aor51HI	AGCGCT	1 cut(s)	835
AoxI	GGCC	7 cut(s)	1037, 1216, 1994, 2294, 2605, 2823, 2978
ApaI	GGGCCC	1 cut(s)	2982
ApeKI	GCWGC	2 cut(s)	84, 1022
ApoI	RAATTY	3 cut(s)	348, 1175, 1624
ArsI	GACNNNNNNTTYG	2 cut(s)	2523, 2555
AsiGI	ACCGGT	1 cut(s)	2965
Asp700I	GAANNNNTTC	1 cut(s)	1461
AspLEI	GCGC	7 cut(s)	154, 490, 630, 836, 1305, 2006, 2595
AspS9I	GGNCC	8 cut(s)	271, 1829, 2054, 2156, 2295, 2978, 2979, 3014
AsuC2I	CCSGG	5 cut(s)	345, 382, 735, 1115, 1116
AsuHPI	GGTGA	7 cut(s)	464, 902, 1252, 2066, 2495, 2884, 2954
AsuII	TTCGAA	1 cut(s)	747
AvaI	CYCGRG	2 cut(s)	575, 1114
AvaII	GGWCC	5 cut(s)	271, 1829, 2054, 2156, 3014
BaeGI	GKGCMC	2 cut(s)	2587, 2982
BalI	TGGCCA	3 cut(s)	1039, 2607, 2825
BamHI	GGATCC	1 cut(s)	665
BanI	GGYRCC	3 cut(s)	2003, 2582, 2592
BanII	GRGCYC	2 cut(s)	576, 2982
BauI	CACGAG	4 cut(s)	359, 507, 1164, 2645
BbsI	GAAGAC	2 cut(s)	188, 2178
Bbv12I	GWGCWC	2 cut(s)	576, 2769
BbvI	GCAGC	2 cut(s)	96, 1034
BceAI	ACGGC	1 cut(s)	530
BciT130I	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BclI	TGATCA	2 cut(s)	190, 454
BcnI	CCSGG	5 cut(s)	345, 382, 735, 1115, 1116
BcoDI	GTCTC	4 cut(s)	98, 648, 1708, 2115
BcuI	ACTAGT	2 cut(s)	370, 1106
BfaI	CTAG	7 cut(s)	371, 467, 1107, 1172, 1392, 2109, 2633
BfmI	CTRYAG	1 cut(s)	2116
BfoI	RGCGCY	4 cut(s)	837, 1306, 2007, 2596
BglII	AGATCT	1 cut(s)	1837
BisI	GCNGC	4 cut(s)	85, 1023, 1161, 2596
BlpI	GCTNAGC	1 cut(s)	1142
BlsI	GCNGC	4 cut(s)	86, 1024, 1162, 2597
BmcAI	AGTACT	1 cut(s)	197
Bme18I	GGWCC	5 cut(s)	271, 1829, 2054, 2156, 3014
BmeT110I	CYCGRG	2 cut(s)	575, 1114
BmgT120I	GGNCC	8 cut(s)	271, 1829, 2054, 2156, 2295, 2978, 2979, 3014
BmrI	ACTGGG	1 cut(s)	2489
BmuI	ACTGGG	1 cut(s)	2489
BpiI	GAAGAC	2 cut(s)	188, 2178
BpmI	CTGGAG	2 cut(s)	1854, 2424
Bpu1102I	GCTNAGC	1 cut(s)	1142
Bpu14I	TTCGAA	1 cut(s)	747
BpuEI	CTTGAG	2 cut(s)	1607, 2480
BpuMI	CCSGG	5 cut(s)	345, 382, 735, 1115, 1116
BsaAI	YACGTR	2 cut(s)	1642, 1987
BsaHI	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
BsaJI	CCNNGG	4 cut(s)	1114, 2090, 2588, 3018
BsaWI	WCCGGW	2 cut(s)	712, 2965
BsaXI	ACNNNNNCTCC	4 cut(s)	626, 656, 1893, 1923
Bsc4I	CCNNNNNNNGG	8 cut(s)	512, 576, 734, 775, 1811, 2321, 2972, 2987
Bse118I	RCCGGY	2 cut(s)	1262, 2965
Bse1I	ACTGG	9 cut(s)	901, 1030, 1099, 1414, 2407, 2484, 2608, 2754, 2826
BseBI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BseDI	CCNNGG	4 cut(s)	1114, 2090, 2588, 3018
BseGI	GGATG	4 cut(s)	521, 1514, 1887, 2758
BseLI	CCNNNNNNNGG	8 cut(s)	512, 576, 734, 775, 1811, 2321, 2972, 2987
BseMII	CTCAG	6 cut(s)	294, 704, 1041, 1314, 1512, 2394
BseNI	ACTGG	9 cut(s)	901, 1030, 1099, 1414, 2407, 2484, 2608, 2754, 2826
BseSI	GKGCMC	2 cut(s)	2587, 2982
BseXI	GCAGC	2 cut(s)	96, 1034
BseYI	CCCAGC	1 cut(s)	1965
Bsh1285I	CGRYCG	2 cut(s)	970, 2154
BshFI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BshNI	GGYRCC	3 cut(s)	2003, 2582, 2592
BshTI	ACCGGT	1 cut(s)	2965
BsiEI	CGRYCG	2 cut(s)	970, 2154
BsiHKAI	GWGCWC	2 cut(s)	576, 2769
BsiHKCI	CYCGRG	2 cut(s)	575, 1114
BsiSI	CCGG	8 cut(s)	344, 381, 713, 735, 1115, 1263, 1993, 2966
BslFI	GGGAC	4 cut(s)	752, 1671, 1881, 2270
BslI	CCNNNNNNNGG	8 cut(s)	512, 576, 734, 775, 1811, 2321, 2972, 2987
BsmAI	GTCTC	4 cut(s)	98, 648, 1708, 2115
BsmBI	CGTCTC	1 cut(s)	98
BsmFI	GGGAC	4 cut(s)	752, 1671, 1881, 2270
BsmI	GAATGC	4 cut(s)	154, 219, 311, 2636
BsnI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BsoBI	CYCGRG	2 cut(s)	575, 1114
Bsp119I	TTCGAA	1 cut(s)	747
Bsp120I	GGGCCC	1 cut(s)	2978
Bsp1286I	GDGCHC	4 cut(s)	576, 2587, 2769, 2982
Bsp1720I	GCTNAGC	1 cut(s)	1142
Bsp19I	CCATGG	2 cut(s)	2090, 3018
BspANI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BspCNI	CTCAG	6 cut(s)	295, 703, 1042, 1315, 1513, 2393
BspPI	GGATC	9 cut(s)	214, 272, 521, 660, 673, 2102, 2416, 2462, 2798
BspQI	GCTCTTC	2 cut(s)	1469, 1763
BspT104I	TTCGAA	1 cut(s)	747
BspT107I	GGYRCC	3 cut(s)	2003, 2582, 2592
BsrBI	CCGCTC	2 cut(s)	1349, 1773
BsrFI	RCCGGY	2 cut(s)	1262, 2965
BsrI	ACTGG	9 cut(s)	901, 1030, 1099, 1414, 2407, 2484, 2608, 2754, 2826
BssAI	RCCGGY	2 cut(s)	1262, 2965
BssECI	CCNNGG	4 cut(s)	1114, 2090, 2588, 3018
BssNI	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
BssSI	CACGAG	4 cut(s)	359, 507, 1164, 2645
BssT1I	CCWWGG	2 cut(s)	2090, 3018
Bst2BI	CACGAG	4 cut(s)	359, 507, 1164, 2645
Bst2UI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
Bst6I	CTCTTC	4 cut(s)	792, 1469, 1763, 2745
BstACI	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
BstBAI	YACGTR	2 cut(s)	1642, 1987
BstBI	TTCGAA	1 cut(s)	747
BstC8I	GCNNGC	5 cut(s)	552, 1139, 1971, 2133, 2714
BstDEI	CTNAG	8 cut(s)	303, 690, 1050, 1142, 1323, 1521, 2339, 2380
BstDSI	CCRYGG	2 cut(s)	2090, 3018
BstEII	GGTNACC	1 cut(s)	470
BstENI	CCTNNNNNAGG	1 cut(s)	2319
BstF5I	GGATG	4 cut(s)	521, 1514, 1887, 2758
BstH2I	RGCGCY	4 cut(s)	837, 1306, 2007, 2596
BstHHI	GCGC	7 cut(s)	154, 490, 630, 836, 1305, 2006, 2595
BstMAI	GTCTC	4 cut(s)	98, 648, 1708, 2115
BstMCI	CGRYCG	2 cut(s)	970, 2154
BstNI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BstNSI	RCATGY	2 cut(s)	2135, 2526
BstPI	GGTNACC	1 cut(s)	470
BstSFI	CTRYAG	1 cut(s)	2116
BstSLI	GKGCMC	2 cut(s)	2587, 2982
BstSNI	TACGTA	2 cut(s)	1642, 1987
BstV1I	GCAGC	2 cut(s)	96, 1034
BstV2I	GAAGAC	2 cut(s)	188, 2178
BstX2I	RGATCY	4 cut(s)	665, 1837, 2094, 2803
BstXI	CCANNNNNNTGG	2 cut(s)	1686, 1722
BstYI	RGATCY	4 cut(s)	665, 1837, 2094, 2803
BsuRI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BtgI	CCRYGG	2 cut(s)	2090, 3018
BtgZI	GCGATG	1 cut(s)	1968
BtsCI	GGATG	4 cut(s)	521, 1514, 1887, 2758
BtsI	GCAGTG	1 cut(s)	1266
BtsIMutI	CAGTG	6 cut(s)	1040, 1266, 1421, 2182, 2400, 2819
Cac8I	GCNNGC	5 cut(s)	552, 1139, 1971, 2133, 2714
CaiI	CAGNNNCTG	3 cut(s)	1334, 1440, 2749
CfoI	GCGC	7 cut(s)	154, 490, 630, 836, 1305, 2006, 2595
Cfr10I	RCCGGY	2 cut(s)	1262, 2965
Cfr13I	GGNCC	8 cut(s)	271, 1829, 2054, 2156, 2295, 2978, 2979, 3014
Cfr9I	CCCGGG	1 cut(s)	1114
CseI	GACGC	2 cut(s)	511, 2441
Csp6I	GTAC	6 cut(s)	196, 904, 1389, 1592, 1910, 2957
CspAI	ACCGGT	1 cut(s)	2965
CspCI	CAANNNNNGTGG	2 cut(s)	308, 343
CviQI	GTAC	6 cut(s)	196, 904, 1389, 1592, 1910, 2957
DdeI	CTNAG	8 cut(s)	303, 690, 1050, 1142, 1323, 1521, 2339, 2380
DinI	GGCGCC	2 cut(s)	2005, 2594
DraI	TTTAAA	1 cut(s)	2040
DrdI	GACNNNNNNGTC	1 cut(s)	764
DseDI	GACNNNNNNGTC	1 cut(s)	764
EaeI	YGGCCR	3 cut(s)	1037, 2605, 2823
Eam1104I	CTCTTC	4 cut(s)	792, 1469, 1763, 2745
EarI	CTCTTC	4 cut(s)	792, 1469, 1763, 2745
EciI	GGCGGA	1 cut(s)	26
Ecl136II	GAGCTC	1 cut(s)	574
Eco105I	TACGTA	2 cut(s)	1642, 1987
Eco130I	CCWWGG	2 cut(s)	2090, 3018
Eco24I	GRGCYC	2 cut(s)	576, 2982
Eco47I	GGWCC	5 cut(s)	271, 1829, 2054, 2156, 3014
Eco47III	AGCGCT	1 cut(s)	835
Eco53kI	GAGCTC	1 cut(s)	574
Eco57I	CTGAAG	3 cut(s)	822, 2726, 2858
Eco88I	CYCGRG	2 cut(s)	575, 1114
Eco91I	GGTNACC	1 cut(s)	470
EcoICRI	GAGCTC	1 cut(s)	574
EcoNI	CCTNNNNNAGG	1 cut(s)	2319
EcoO109I	RGGNCCY	1 cut(s)	2978
EcoO65I	GGTNACC	1 cut(s)	470
EcoRI	GAATTC	1 cut(s)	348
EcoRII	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
EcoT14I	CCWWGG	2 cut(s)	2090, 3018
EcoT22I	ATGCAT	1 cut(s)	420
EcoT38I	GRGCYC	2 cut(s)	576, 2982
EgeI	GGCGCC	2 cut(s)	2005, 2594
EheI	GGCGCC	2 cut(s)	2005, 2594
ErhI	CCWWGG	2 cut(s)	2090, 3018
Esp3I	CGTCTC	1 cut(s)	98
FaqI	GGGAC	4 cut(s)	752, 1671, 1881, 2270
FbaI	TGATCA	2 cut(s)	190, 454
FblI	GTMKAC	1 cut(s)	2455
Fnu4HI	GCNGC	4 cut(s)	85, 1023, 1161, 2596
FokI	GGATG	4 cut(s)	528, 1521, 1894, 2765
FriOI	GRGCYC	2 cut(s)	576, 2982
Fsp4HI	GCNGC	4 cut(s)	85, 1023, 1161, 2596
FspBI	CTAG	7 cut(s)	371, 467, 1107, 1172, 1392, 2109, 2633
GlaI	GCGC	7 cut(s)	153, 489, 629, 835, 1304, 2005, 2594
GluI	GCNGC	4 cut(s)	85, 1023, 1161, 2596
GsaI	CCCAGC	1 cut(s)	1969
GsuI	CTGGAG	2 cut(s)	1854, 2424
HaeII	RGCGCY	4 cut(s)	837, 1306, 2007, 2596
HaeIII	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
HapII	CCGG	8 cut(s)	344, 381, 713, 735, 1115, 1263, 1993, 2966
HgaI	GACGC	2 cut(s)	511, 2441
HhaI	GCGC	7 cut(s)	154, 490, 630, 836, 1305, 2006, 2595
Hin1I	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
Hin6I	GCGC	7 cut(s)	152, 488, 628, 834, 1303, 2004, 2593
HinP1I	GCGC	7 cut(s)	152, 488, 628, 834, 1303, 2004, 2593
HincII	GTYRAC	2 cut(s)	333, 718
HindII	GTYRAC	2 cut(s)	333, 718
HindIII	AAGCTT	2 cut(s)	552, 1971
HpaI	GTTAAC	2 cut(s)	333, 718
HpaII	CCGG	8 cut(s)	344, 381, 713, 735, 1115, 1263, 1993, 2966
HphI	GGTGA	7 cut(s)	464, 902, 1252, 2066, 2495, 2884, 2954
Hpy99I	CGWCG	2 cut(s)	764, 2987
HpyAV	CCTTC	6 cut(s)	1117, 1182, 2247, 2522, 2780, 2869
HpyCH4IV	ACGT	6 cut(s)	245, 762, 873, 1641, 1759, 1986
HpyF3I	CTNAG	8 cut(s)	303, 690, 1050, 1142, 1323, 1521, 2339, 2380
HpySE526I	ACGT	6 cut(s)	245, 762, 873, 1641, 1759, 1986
Hsp92I	GRCGYC	4 cut(s)	503, 2004, 2452, 2593
HspAI	GCGC	7 cut(s)	152, 488, 628, 834, 1303, 2004, 2593
KasI	GGCGCC	2 cut(s)	2003, 2592
Ksp22I	TGATCA	2 cut(s)	190, 454
KspAI	GTTAAC	2 cut(s)	333, 718
LguI	GCTCTTC	2 cut(s)	1469, 1763
LmnI	GCTCC	2 cut(s)	1316, 2772
Lsp1109I	GCAGC	2 cut(s)	96, 1034
MaeI	CTAG	7 cut(s)	371, 467, 1107, 1172, 1392, 2109, 2633
MaeII	ACGT	6 cut(s)	245, 762, 873, 1641, 1759, 1986
MaeIII	GTNAC	8 cut(s)	470, 841, 856, 1258, 1436, 1615, 2104, 2942
MbiI	CCGCTC	2 cut(s)	1349, 1773
MfeI	CAATTG	1 cut(s)	1818
MflI	RGATCY	4 cut(s)	665, 1837, 2094, 2803
MhlI	GDGCHC	4 cut(s)	576, 2587, 2769, 2982
MlsI	TGGCCA	3 cut(s)	1039, 2607, 2825
MluCI	AATT	9 cut(s)	348, 945, 1175, 1624, 1818, 1888, 2326, 2704, 2733
MluNI	TGGCCA	3 cut(s)	1039, 2607, 2825
Mly113I	GGCGCC	2 cut(s)	2004, 2593
MlyI	GAGTC	1 cut(s)	2106
MmeI	TCCRAC	6 cut(s)	184, 228, 1472, 1703, 2036, 2889
Mox20I	TGGCCA	3 cut(s)	1039, 2607, 2825
Mph1103I	ATGCAT	1 cut(s)	420
MroXI	GAANNNNTTC	1 cut(s)	1461
MscI	TGGCCA	3 cut(s)	1039, 2607, 2825
MslI	CAYNNNNRTG	4 cut(s)	165, 446, 1251, 2787
Msp20I	TGGCCA	3 cut(s)	1039, 2607, 2825
MspA1I	CMGCKG	1 cut(s)	87
MspI	CCGG	8 cut(s)	344, 381, 713, 735, 1115, 1263, 1993, 2966
MunI	CAATTG	1 cut(s)	1818
Mva1269I	GAATGC	4 cut(s)	154, 219, 311, 2636
MvaI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
NarI	GGCGCC	2 cut(s)	2004, 2593
NciI	CCSGG	5 cut(s)	345, 382, 735, 1115, 1116
NcoI	CCATGG	2 cut(s)	2090, 3018
NmeAIII	GCCGAG	1 cut(s)	595
NmuCI	GTSAC	5 cut(s)	470, 856, 1258, 2104, 2942
NsiI	ATGCAT	1 cut(s)	420
NspI	RCATGY	2 cut(s)	2135, 2526
NspV	TTCGAA	1 cut(s)	747
PaeI	GCATGC	1 cut(s)	2135
PciSI	GCTCTTC	2 cut(s)	1469, 1763
PctI	GAATGC	4 cut(s)	154, 219, 311, 2636
PdmI	GAANNNNTTC	1 cut(s)	1461
PflFI	GACNNNGTC	1 cut(s)	764
PflMI	CCANNNNNTGG	1 cut(s)	1811
PfoI	TCCNGGA	2 cut(s)	343, 774
PinAI	ACCGGT	1 cut(s)	2965
PkrI	GCNGC	4 cut(s)	86, 1024, 1162, 2597
Ple19I	CGATCG	1 cut(s)	2154
PleI	GAGTC	1 cut(s)	2106
PluTI	GGCGCC	2 cut(s)	2007, 2596
PpsI	GAGTC	1 cut(s)	2106
Ppu21I	YACGTR	2 cut(s)	1642, 1987
Psp124BI	GAGCTC	1 cut(s)	576
Psp6I	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
PspEI	GGTNACC	1 cut(s)	470
PspFI	CCCAGC	1 cut(s)	1965
PspGI	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
PspOMI	GGGCCC	1 cut(s)	2978
PspPI	GGNCC	8 cut(s)	271, 1829, 2054, 2156, 2295, 2978, 2979, 3014
PstNI	CAGNNNCTG	3 cut(s)	1334, 1440, 2749
PsuI	RGATCY	4 cut(s)	665, 1837, 2094, 2803
PsyI	GACNNNGTC	1 cut(s)	764
PvuI	CGATCG	1 cut(s)	2154
RsaI	GTAC	6 cut(s)	197, 905, 1390, 1593, 1911, 2958
RsaNI	GTAC	6 cut(s)	196, 904, 1389, 1592, 1910, 2957
RseI	CAYNNNNRTG	4 cut(s)	165, 446, 1251, 2787
SacI	GAGCTC	1 cut(s)	576
SapI	GCTCTTC	2 cut(s)	1469, 1763
SatI	GCNGC	4 cut(s)	85, 1023, 1161, 2596
Sau96I	GGNCC	8 cut(s)	271, 1829, 2054, 2156, 2295, 2978, 2979, 3014
ScaI	AGTACT	1 cut(s)	197
SchI	GAGTC	1 cut(s)	2106
SduI	GDGCHC	4 cut(s)	576, 2587, 2769, 2982
SfcI	CTRYAG	1 cut(s)	2116
SfoI	GGCGCC	2 cut(s)	2005, 2594
SfuI	TTCGAA	1 cut(s)	747
SinI	GGWCC	5 cut(s)	271, 1829, 2054, 2156, 3014
SmaI	CCCGGG	1 cut(s)	1116
SmiMI	CAYNNNNRTG	4 cut(s)	165, 446, 1251, 2787
SmlI	CTYRAG	2 cut(s)	1586, 2459
SmoI	CTYRAG	2 cut(s)	1586, 2459
SnaBI	TACGTA	2 cut(s)	1642, 1987
SpeI	ACTAGT	2 cut(s)	370, 1106
SphI	GCATGC	1 cut(s)	2135
Sse9I	AATT	9 cut(s)	348, 945, 1175, 1624, 1818, 1888, 2326, 2704, 2733
SspDI	GGCGCC	2 cut(s)	2003, 2592
SspI	AATATT	2 cut(s)	1582, 2813
SspMI	CTAG	7 cut(s)	371, 467, 1107, 1172, 1392, 2109, 2633
SstI	GAGCTC	1 cut(s)	576
StyI	CCWWGG	2 cut(s)	2090, 3018
TaiI	ACGT	6 cut(s)	248, 765, 876, 1644, 1762, 1989
TaqI	TCGA	7 cut(s)	747, 795, 970, 2511, 2542, 2937, 2985
TaqII	GACCGA	2 cut(s)	2071, 2144
TasI	AATT	9 cut(s)	348, 945, 1175, 1624, 1818, 1888, 2326, 2704, 2733
TatI	WGTACW	2 cut(s)	195, 1388
TauI	GCSGC	2 cut(s)	1163, 2598
TscAI	CASTG	6 cut(s)	1047, 1273, 1421, 2182, 2407, 2826
TseFI	GTSAC	5 cut(s)	470, 856, 1258, 2104, 2942
TseI	GCWGC	2 cut(s)	84, 1022
Tsp45I	GTSAC	5 cut(s)	470, 856, 1258, 2104, 2942
TspDTI	ATGAA	9 cut(s)	131, 438, 675, 817, 1300, 1726, 2251, 2511, 2930
TspGWI	ACGGA	4 cut(s)	263, 1053, 1696, 2265
TspMI	CCCGGG	1 cut(s)	1114
TspRI	CASTG	6 cut(s)	1047, 1273, 1421, 2182, 2407, 2826
Tth111I	GACNNNGTC	1 cut(s)	764
Van91I	CCANNNNNTGG	1 cut(s)	1811
VpaK11BI	GGWCC	5 cut(s)	271, 1829, 2054, 2156, 3014
XagI	CCTNNNNNAGG	1 cut(s)	2319
XapI	RAATTY	3 cut(s)	348, 1175, 1624
XceI	RCATGY	2 cut(s)	2135, 2526
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1973
XmaI	CCCGGG	1 cut(s)	1114
XmiI	GTMKAC	1 cut(s)	2455
XmnI	GAANNNNTTC	1 cut(s)	1461
XspI	CTAG	7 cut(s)	371, 467, 1107, 1172, 1392, 2109, 2633
ZrmI	AGTACT	1 cut(s)	197
Zsp2I	ATGCAT	1 cut(s)	420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.