Rh2CG131100

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004264
Physical Location & Seq
Reverse (-)
11687246 .. 11695221
7976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG131100.1

Sequence Viewer

Length: 2814 bp
ATGCTAAAGCTCGCGGCGTTTTTCGAGCTTGTGATTGTGGTTGTTTGGTTGGGAGTGGCGAGTGTTGATGGAGTTTTTGTGAAGTACCAGACCGGGAGTGGTGTTGTGGAAGGGAAGTTGAATGTTCATTTGGTTGCGCATTCCCATGATGACGTTGGTTGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAATAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTCGTTGAATCGTTGGTTCGTGATCCGAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTTACACAAACTGTGGAAGTTCAAGAACGAGTGCGGAAACTAGTAGATGCTGGCCAGTTAGAGTTTGTAAATGGAGGTTGGTGCATGCATGATGAGGCTACAGTCCATTACATAGACATGATTGACCAAACAACTCTAGGCCACCTTGCTATAAAGCAGCAGTTCAACAAGACACCTCGAGCGGGGTGGCAAATTGATCCGTTCGGACACTCTGCTGTGCAGGGTTACTTACTTGGAGCTGAGCTTGGATTCGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGCACAGCGTAAGGGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTCGTCACTATAGTCCGCCAGATGGTTTCCATTTTGAAGTTTTTGATGACTTTGTCCCTGTGCAGGATAATCCGCTTATTTTTGACTACAATGTTGAACTGCGTGTTAATGATTTTATTACTGCTGCAATCACCCAAGCAAATGTGACTAGGGGAAATCACATGATGTGGACAATGGGAGATGATTTCCAGTACCAATATGCTGAATCTTGGTTCAAACAAATGGATAAGTTAATTCACTATGTTAATAAGGATGGCCGAGTTAATGCCTTATATTCTACACCGTCTCTCTACACTGATGCTAAAAATGCAGCAAATCAATCATGGCCATTAAAAACTGAGGATTATTTTCCGTATGCAGATATAATAAATGCTTATTGGACCGGTTATTTTACTAGTCGCCCGGGTTTGAAAAGATATATTCGATCACTGAGCGGATATTATTTGGCAGCAAGAGAACTAGAATTTCTGGTTGGAAAGAGAGCAAATGGTTCAAGTACCTATTCCCTTGGAGATGCTTTAGGAATTGCACAGCACCACGATGCTGCCACTGGCACTGCTAAACAACATACAACAAATGACTATGTAAAGCGCCTGTCAATTGGTGTCACTGAGGCAGAAGCTGTTGTCAGTTCTGCTCTGTCGTGCCTAACTACAAATAAATCTGGAGATCAATGTAAAGACCCTGCAGTTACTTTTAGCCAGTGTCGACTACTCAATATCAGTTTCTGCCCTCCAACGGAGGAAGATATTCCAGAAGGGAAGAGCTTGGTTGTTGTGGCATATAATCCTCTTGGATGGAATCGCACTGAGATTGTTAGGATACCTGTTAATTATGATGATCTTGTTGTTCAAGATTCCTCTGGAAACATCCTCGAGACACAATATGTGAGTGTGGATAATGTTACAAGCAATCTAAGAACATTTTATACAGAAGCCTACTTGGGACAGTCATCAAAACAAATTCCCAAGTACTGGCTAATCTTTCAAGCCTCTGTGTCACCACTTGGTTGGGACACATACTTCATCTCTAGAGGAGCTACCAAAGGGAAAAGAAGAGTTGGACTTCTCTCTGTGAAGGACAGTCCACAGAATGAGACCATTGAAATTGGACCAGGAGATCTGAAGATGTCCTTTTCTTCTGCTTCTGGACAACTCAAGCGGATGTATAATTCTAAAACAGGAGTTGATGTACCAATACAACAGAGCTACCTCTGGTATGGTTCAAGCACCGATGGCCAGGCTTCAGGTGCATATATATTCCGGCCTAATGGTGCCCCTCCAACTATTGTGTCAAGATCTGTACCCTTGAAGGTCTTTCGTGGACCATTATTCGATGAGATTCACCAAGAATTCAGTTCATGGATCTATCAGGTCATTAGAGTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACTATTGGTCCAATTCCTACAGACGACGGTGTTGGTAAAGAGGTCATCACACGAATGACAGCAAACATGGAGACTAACAAGATGTTTTACACCGATTCCAATGGGAGGGATTTCATTAAAAGGGTACGAGATTATAGAGCAGACTGGAACCTTTCAGTTAATCAACCTGTTGCAGGAAACTACTATCCACTAAACCTTGGGATCTTTACTTCGGATAACAAATCTGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCCAGCATTCAAGACGGACAAGTAGAGATGATGCTACATAGGTTCGATTTTGACTCGCTGTTTTATCAGCATTTCAGATCAAGTAATTCCTTTATTCACAATCTAATTCTTCCTGTTATAAACTCAAGGCGTCTACTGGTTGATGATGGTAGAGGGGTGGGTGAAGCCCTTGATGAAACAGTGTGCATCAAGAATAATACATGTCAAGGACTAACGGTTCGAGGAAACTATTATATGAGCATAAACCAGTTCGGGGATGGTGCCCAATGGCGTCGGACAACTGGACAAGAAGTTTACTCGCCACTTCTTCTAGCTTTCACCCATGAGAACTTGGAGAATTGGACTGCATCTCATTTAACAAAAGGGTCTGTCATGGATTCAAGTTACAGCTTGCCTCCCAATGTTGCTTTGATTACTCTTCAGGTGACTGTATATTAA

Protein Analysis

937

Amino Acids

105.56

Weight (kDa)

5.8

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 41 - 352 2.5e-77 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 357 - 449 4.6e-18 Alpha mannosidase middle domain
Laman-like_dom PF21260 539 - 575 8.5e-06 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 605 - 807 5.9e-43 Glycosyl hydrolases family 38 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2495
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 1970, 2636
AccB7I CCANNNNNTGG 1 cut(s) 1671
AccBSI CCGCTC 2 cut(s) 497, 1131
AccI GTMKAC 2 cut(s) 1405, 2509
AccII CGCG 1 cut(s) 14
AciI CCGC 7 cut(s) 14, 349, 497, 713, 770, 1131, 1858
AclWI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AcoI YGGCCR 4 cut(s) 367, 952, 1022, 1933
AcsI RAATTY 3 cut(s) 1160, 1659, 2048
AcuI CTGAAG 3 cut(s) 1841, 1926, 2780
AcyI GRCGYC 2 cut(s) 2506, 2647
AfaI GTAC 9 cut(s) 86, 182, 576, 890, 1195, 1670, 1890, 2001, 2241
AfiI CCNNNNNNNGG 8 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2629
AflIII ACRYGT 1 cut(s) 2576
AgeI ACCGGT 1 cut(s) 1079
AhlI ACTAGT 2 cut(s) 355, 1091
AjnI CCWGG 2 cut(s) 1810, 1935
AjuI GAANNNNNNNTTGG 6 cut(s) 113, 145, 1152, 1184, 2103, 2135
AloI GAACNNNNNNTCC 2 cut(s) 2577, 2609
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
AlwI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AlwNI CAGNNNCTG 2 cut(s) 1319, 1425
Ama87I CYCGRG 3 cut(s) 492, 1099, 1571
AoxI GGCC 6 cut(s) 367, 454, 952, 1022, 1933, 1961
ApeKI GCWGC 5 cut(s) 472, 821, 1007, 1145, 1241
ApoI RAATTY 3 cut(s) 1160, 1659, 2048
ArsI GACNNNNNNTTYG 2 cut(s) 2577, 2609
AsiGI ACCGGT 1 cut(s) 1079
Asp700I GAANNNNTTC 1 cut(s) 1446
AspLEI GCGC 2 cut(s) 139, 1290
AspS9I GGNCC 4 cut(s) 1077, 1808, 2021, 2123
AsuC2I CCSGG 3 cut(s) 94, 1100, 1101
AsuHPI GGTGA 6 cut(s) 820, 1689, 2033, 2549, 2686, 2812
AvaI CYCGRG 3 cut(s) 492, 1099, 1571
AvaII GGWCC 4 cut(s) 1077, 1808, 2021, 2123
BaeGI GKGCMC 2 cut(s) 1975, 2641
BalI TGGCCA 3 cut(s) 369, 1024, 1935
BamHI GGATCC 2 cut(s) 650, 2375
BanI GGYRCC 2 cut(s) 1970, 2636
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 5 cut(s) 484, 808, 1019, 1157, 1228
BccI CCATC 9 cut(s) 62, 286, 303, 713, 944, 1488, 1925, 2516, 2627
BciT130I CCWGG 2 cut(s) 1812, 1937
BciVI GTATCC 1 cut(s) 1512
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 3 cut(s) 94, 1100, 1101
BcoDI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BcuI ACTAGT 2 cut(s) 355, 1091
BfaI CTAG 7 cut(s) 356, 452, 846, 1092, 1157, 1728, 2687
BfmI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BfoI RGCGCY 1 cut(s) 1291
BfuI GTATCC 1 cut(s) 1512
BglII AGATCT 2 cut(s) 1816, 1994
BisI GCNGC 6 cut(s) 15, 473, 822, 1008, 1146, 1242
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 6 cut(s) 16, 474, 823, 1009, 1147, 1243
BmcAI AGTACT 2 cut(s) 182, 1670
Bme1390I CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
Bme18I GGWCC 4 cut(s) 1077, 1808, 2021, 2123
BmeT110I CYCGRG 3 cut(s) 492, 1099, 1571
BmgT120I GGNCC 4 cut(s) 1077, 1808, 2021, 2123
BmiI GGNNCC 5 cut(s) 652, 1972, 2264, 2377, 2638
BmrFI CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
BmsI GCATC 7 cut(s) 352, 985, 1201, 1228, 2397, 2571, 2732
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 1383, 2391
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 1838, 2485
BpuMI CCSGG 3 cut(s) 94, 1100, 1101
BsaHI GRCGYC 2 cut(s) 2506, 2647
BsaI GGTCTC 1 cut(s) 1787
BsaJI CCNNGG 3 cut(s) 1099, 1204, 2311
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1872, 1902
Bsc4I CCNNNNNNNGG 8 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2629
Bse118I RCCGGY 1 cut(s) 1079
BseBI CCWGG 2 cut(s) 1812, 1937
BseDI CCNNGG 3 cut(s) 1099, 1204, 2311
BseGI GGATG 5 cut(s) 955, 1499, 1566, 1866, 2638
BseLI CCNNNNNNNGG 8 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2629
BseMII CTCAG 9 cut(s) 279, 546, 689, 1026, 1118, 1299, 1497, 2331, 2361
BseRI GAGGAG 1 cut(s) 1746
BseSI GKGCMC 2 cut(s) 1975, 2641
BseXI GCAGC 5 cut(s) 484, 808, 1019, 1157, 1228
BsgI GTGCAG 2 cut(s) 554, 779
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BshNI GGYRCC 2 cut(s) 1970, 2636
BshTI ACCGGT 1 cut(s) 1079
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 3 cut(s) 492, 1099, 1571
BsiSI CCGG 4 cut(s) 93, 1080, 1100, 1960
BslFI GGGAC 3 cut(s) 737, 1656, 1724
BslI CCNNNNNNNGG 8 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2629
BsmAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 3 cut(s) 737, 1656, 1724
BsmI GAATGC 3 cut(s) 139, 204, 2382
BsnI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
Bso31I GGTCTC 1 cut(s) 1787
BsoBI CYCGRG 3 cut(s) 492, 1099, 1571
Bsp1286I GDGCHC 3 cut(s) 610, 1975, 2641
Bsp1407I TGTACA 1 cut(s) 574
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 7 cut(s) 14, 349, 497, 713, 770, 1131, 1858
BspANI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BspCNI CTCAG 9 cut(s) 280, 547, 688, 1027, 1119, 1300, 1498, 2332, 2360
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 5 cut(s) 652, 1972, 2264, 2377, 2638
BspMAI CTGCAG 1 cut(s) 1387
BspPI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 2 cut(s) 1970, 2636
BspTNI GGTCTC 1 cut(s) 1787
BsrBI CCGCTC 2 cut(s) 497, 1131
BsrFI RCCGGY 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 3 cut(s) 1099, 1204, 2311
BssNI GRCGYC 2 cut(s) 2506, 2647
BssT1I CCWWGG 2 cut(s) 1204, 2311
Bst2UI CCWGG 2 cut(s) 1812, 1937
Bst6I CTCTTC 3 cut(s) 1454, 1747, 2799
BstACI GRCGYC 2 cut(s) 2506, 2647
BstAUI TGTACA 1 cut(s) 574
BstC8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2768
BstENI CCTNNNNNAGG 1 cut(s) 2286
BstF5I GGATG 5 cut(s) 955, 1499, 1566, 1866, 2638
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 1 cut(s) 1291
BstHHI GCGC 2 cut(s) 139, 1290
BstMAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BstMWI GCNNNNNNNGC 4 cut(s) 293, 1004, 1932, 1946
BstNI CCWGG 2 cut(s) 1812, 1937
BstNSI RCATGY 3 cut(s) 403, 2102, 2580
BstSCI CCNGG 5 cut(s) 92, 1098, 1099, 1810, 1935
BstSFI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BstSLI GKGCMC 2 cut(s) 1975, 2641
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 5 cut(s) 484, 808, 1019, 1157, 1228
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BstXI CCANNNNNNTGG 1 cut(s) 1707
BstYI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BsuI GTATCC 1 cut(s) 1512
BsuRI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BtsCI GGATG 5 cut(s) 955, 1499, 1566, 1866, 2638
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 9 cut(s) 990, 1124, 1245, 1251, 1305, 1406, 1503, 2367, 2562
Cac8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2768
CaiI CAGNNNCTG 2 cut(s) 1319, 1425
CfoI GCGC 2 cut(s) 139, 1290
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 4 cut(s) 1077, 1808, 2021, 2123
Cfr9I CCCGGG 1 cut(s) 1099
CseI GACGC 2 cut(s) 2495, 2636
Csp6I GTAC 9 cut(s) 85, 181, 575, 889, 1194, 1669, 1889, 2000, 2240
CspAI ACCGGT 1 cut(s) 1079
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviQI GTAC 9 cut(s) 85, 181, 575, 889, 1194, 1669, 1889, 2000, 2240
EaeI YGGCCR 4 cut(s) 367, 952, 1022, 1933
Eam1104I CTCTTC 3 cut(s) 1454, 1747, 2799
EarI CTCTTC 3 cut(s) 1454, 1747, 2799
EciI GGCGGA 1 cut(s) 702
Eco130I CCWWGG 2 cut(s) 1204, 2311
Eco31I GGTCTC 1 cut(s) 1787
Eco47I GGWCC 4 cut(s) 1077, 1808, 2021, 2123
Eco57I CTGAAG 3 cut(s) 1841, 1926, 2780
Eco88I CYCGRG 3 cut(s) 492, 1099, 1571
EcoNI CCTNNNNNAGG 1 cut(s) 2286
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 2 cut(s) 1810, 1935
EcoT14I CCWWGG 2 cut(s) 1204, 2311
EcoT22I ATGCAT 1 cut(s) 405
ErhI CCWWGG 2 cut(s) 1204, 2311
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 1814, 1846
FaqI GGGAC 3 cut(s) 737, 1656, 1724
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 2 cut(s) 1405, 2509
Fnu4HI GCNGC 6 cut(s) 15, 473, 822, 1008, 1146, 1242
FokI GGATG 5 cut(s) 962, 1506, 1553, 1873, 2645
Fsp4HI GCNGC 6 cut(s) 15, 473, 822, 1008, 1146, 1242
FspBI CTAG 7 cut(s) 356, 452, 846, 1092, 1157, 1728, 2687
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 2 cut(s) 138, 1289
GluI GCNGC 6 cut(s) 15, 473, 822, 1008, 1146, 1242
GsuI CTGGAG 2 cut(s) 1383, 2391
HaeII RGCGCY 1 cut(s) 1291
HaeIII GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
HapII CCGG 4 cut(s) 93, 1080, 1100, 1960
HgaI GACGC 2 cut(s) 2495, 2636
HhaI GCGC 2 cut(s) 139, 1290
Hin1I GRCGYC 2 cut(s) 2506, 2647
Hin6I GCGC 2 cut(s) 137, 1288
HinP1I GCGC 2 cut(s) 137, 1288
HincII GTYRAC 1 cut(s) 1406
HindII GTYRAC 1 cut(s) 1406
HpaII CCGG 4 cut(s) 93, 1080, 1100, 1960
HphI GGTGA 6 cut(s) 820, 1689, 2033, 2549, 2686, 2812
Hpy166II GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2510, 2671
Hpy188I TCNGA 8 cut(s) 267, 521, 678, 1821, 2329, 2341, 2453, 2652
Hpy8I GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2510, 2671
Hpy99I CGWCG 2 cut(s) 2144, 2652
HpyAV CCTTC 4 cut(s) 104, 1448, 1768, 2002
HpyCH4IV ACGT 2 cut(s) 153, 230
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 1004, 1932, 1946
HpySE526I ACGT 2 cut(s) 153, 230
Hsp92I GRCGYC 2 cut(s) 2506, 2647
HspAI GCGC 2 cut(s) 137, 1288
Ksp22I TGATCA 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 2 cut(s) 551, 1733
Lsp1109I GCAGC 5 cut(s) 484, 808, 1019, 1157, 1228
LweI GCATC 7 cut(s) 352, 985, 1201, 1228, 2397, 2571, 2732
MaeI CTAG 7 cut(s) 356, 452, 846, 1092, 1157, 1728, 2687
MaeII ACGT 2 cut(s) 153, 230
MaeIII GTNAC 9 cut(s) 539, 701, 841, 1303, 1387, 1600, 1695, 2759, 2800
MbiI CCGCTC 2 cut(s) 497, 1131
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
MhlI GDGCHC 3 cut(s) 610, 1975, 2641
MlsI TGGCCA 3 cut(s) 369, 1024, 1935
MluNI TGGCCA 3 cut(s) 369, 1024, 1935
MlyI GAGTC 1 cut(s) 2423
MmeI TCCRAC 7 cut(s) 169, 213, 1150, 1457, 1738, 2003, 2630
Mox20I TGGCCA 3 cut(s) 369, 1024, 1935
Mph1103I ATGCAT 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 3 cut(s) 369, 1024, 1935
MslI CAYNNNNRTG 4 cut(s) 144, 431, 1236, 2168
Msp20I TGGCCA 3 cut(s) 369, 1024, 1935
MspI CCGG 4 cut(s) 93, 1080, 1100, 1960
MspR9I CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 3 cut(s) 139, 204, 2382
MvaI CCWGG 2 cut(s) 1812, 1937
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 4 cut(s) 293, 1004, 1932, 1946
NciI CCSGG 3 cut(s) 94, 1100, 1101
NlaIV GGNNCC 5 cut(s) 652, 1972, 2264, 2377, 2638
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 5 cut(s) 701, 841, 1303, 1695, 2800
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 403, 2102, 2580
PaeI GCATGC 2 cut(s) 403, 2102
PaeR7I CTCGAG 2 cut(s) 492, 1571
PciI ACATGT 1 cut(s) 2576
PciSI GCTCTTC 1 cut(s) 1454
PctI GAATGC 3 cut(s) 139, 204, 2382
PdmI GAANNNNTTC 1 cut(s) 1446
PflFI GACNNNGTC 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 1671
PinAI ACCGGT 1 cut(s) 1079
PkrI GCNGC 6 cut(s) 16, 474, 823, 1009, 1147, 1243
PleI GAGTC 1 cut(s) 2423
PpsI GAGTC 1 cut(s) 2423
PscI ACATGT 1 cut(s) 2576
PsiI TTATAA 1 cut(s) 2495
Psp6I CCWGG 2 cut(s) 1810, 1935
PspGI CCWGG 2 cut(s) 1810, 1935
PspN4I GGNNCC 5 cut(s) 652, 1972, 2264, 2377, 2638
PspPI GGNCC 4 cut(s) 1077, 1808, 2021, 2123
PspXI VCTCGAGB 1 cut(s) 492
PsrI GAACNNNNNNTAC 2 cut(s) 1609, 1641
PstI CTGCAG 1 cut(s) 1387
PstNI CAGNNNCTG 2 cut(s) 1319, 1425
PsuI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
PsyI GACNNNGTC 1 cut(s) 749
RsaI GTAC 9 cut(s) 86, 182, 576, 890, 1195, 1670, 1890, 2001, 2241
RsaNI GTAC 9 cut(s) 85, 181, 575, 889, 1194, 1669, 1889, 2000, 2240
RseI CAYNNNNRTG 4 cut(s) 144, 431, 1236, 2168
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 1 cut(s) 1454
SatI GCNGC 6 cut(s) 15, 473, 822, 1008, 1146, 1242
Sau96I GGNCC 4 cut(s) 1077, 1808, 2021, 2123
ScaI AGTACT 2 cut(s) 182, 1670
SchI GAGTC 1 cut(s) 2423
ScrFI CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
SduI GDGCHC 3 cut(s) 610, 1975, 2641
SfaNI GCATC 7 cut(s) 352, 985, 1201, 1228, 2397, 2571, 2732
SfcI CTRYAG 4 cut(s) 414, 706, 1383, 2133
Sfr274I CTCGAG 2 cut(s) 492, 1571
SinI GGWCC 4 cut(s) 1077, 1808, 2021, 2123
SlaI CTCGAG 2 cut(s) 492, 1571
SmaI CCCGGG 1 cut(s) 1101
SmiMI CAYNNNNRTG 4 cut(s) 144, 431, 1236, 2168
SmlI CTYRAG 4 cut(s) 492, 1571, 1853, 2500
SmoI CTYRAG 4 cut(s) 492, 1571, 1853, 2500
SpeI ACTAGT 2 cut(s) 355, 1091
SphI GCATGC 2 cut(s) 403, 2102
SsiI CCGC 7 cut(s) 14, 349, 497, 713, 770, 1131, 1858
SspMI CTAG 7 cut(s) 356, 452, 846, 1092, 1157, 1728, 2687
StyD4I CCNGG 5 cut(s) 92, 1098, 1099, 1810, 1935
StyI CCWWGG 2 cut(s) 1204, 2311
TaiI ACGT 2 cut(s) 156, 233
TaqI TCGA 9 cut(s) 24, 493, 567, 1120, 1405, 1572, 2031, 2421, 2596
TatI WGTACW 3 cut(s) 180, 574, 1668
TauI GCSGC 1 cut(s) 17
TscAI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2562
TseFI GTSAC 5 cut(s) 701, 841, 1303, 1695, 2800
TseI GCWGC 5 cut(s) 472, 821, 1007, 1145, 1241
Tsp45I GTSAC 5 cut(s) 701, 841, 1303, 1695, 2800
TspDTI ATGAA 6 cut(s) 116, 660, 1711, 2046, 2218, 2565
TspGWI ACGGA 4 cut(s) 504, 1038, 1451, 2406
TspMI CCCGGG 1 cut(s) 1099
TspRI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2562
Tth111I GACNNNGTC 1 cut(s) 749
Van91I CCANNNNNTGG 1 cut(s) 1671
VpaK11BI GGWCC 4 cut(s) 1077, 1808, 2021, 2123
XagI CCTNNNNNAGG 1 cut(s) 2286
XapI RAATTY 3 cut(s) 1160, 1659, 2048
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 3 cut(s) 403, 2102, 2580
XcmI CCANNNNNNNNNTGG 3 cut(s) 95, 152, 2630
XhoI CTCGAG 2 cut(s) 492, 1571
XmaI CCCGGG 1 cut(s) 1099
XmiI GTMKAC 2 cut(s) 1405, 2509
XmnI GAANNNNTTC 1 cut(s) 1446
XspI CTAG 7 cut(s) 356, 452, 846, 1092, 1157, 1728, 2687
ZrmI AGTACT 2 cut(s) 182, 1670
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.