Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004264

Physical Location & Seq

Genomic Coordinates Reverse (-)

10415094 .. 10421604

Gene Structure 6511 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1128700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGGAAAGGCGGAAACTTTGGAGTTGGGTTTTCGGCTTGTGGTTGTTTTGGTTGTGGGTTGTTTATGTAGGGAGGCTGTTGGCGGCGGTTTTGTGAAGTATGAGACGGGGAGTGGCGTGGTGGAGGGGAAACTGAATGTTCATTTGGTGGCGCATTCGCACGATGATGTCGGGTGGTTGAAGACGGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTTGTTGAATCGTTGGTTCGTGATCCGAACAGGAAGTTTGTGTTTGCTGAGATGGCATTCTTCCAAAGATGGTGGTTAACACAAAGTCCGGAAATTCAAGCTCTCGTGCGAAAACTAGTAGATGCTGGGCAGTTAGAGTTTGTCAATGGTGGTTGGTGTATGCATGATGAAGCAACAACCCATTACATAGACATGATTGATCAAACGACGCTAGGTCACCGTGCAATAAAGGCGCAGTTCAACAAGACACCTCGTGCTGGATGGCAAATTGATCCCTTTGGACACTCTGCCGTGCAAGCTTACTTACTCACCGCTGAGCTCGGGTTTGATTCTGTGCATTTTGCAAGGATCGATTATCAGGACAGAGCGAAGCGCAAGGTGGATAAGTCTCTAGAGGTTATATGGCGTGGATCCAAAACTTTTAGTTCTTCATCTCAGATTTTTGCCAATGCTTTTCCGGTTCACTATAGTCCACCTCCCGGTTTCCATTTCGAAGTATTTGACAACTTCGAACCTGTCCAGGATAATCCTCTTCTGTTCGACTACAATGTTGAACAGCGTGTCAGCGATTTCATCAGCGCTTCACTCACCCAAGCAAATGTGACAAGGACGAATCACATTATGTGGACGATGGGAGATGATTTCCAGTACCAATATGCAGAATCATGGTTCAAACAAATGGACAAGTTAATTCACTATGTTAATAAGGACGGTCGAGTTAATGCCTTGTATTCTACACCATCTATCTATACCGATGCTAAAAATGCAGCAAACCAGTCATGGCCACTGAAAACTGAGGATTATTTTCCGTATGCAGATGCGGTAGATGCTTATTGGACTGGATTTTTCACTAGTCGCCCGGGTTTGAAGGGATATATCCGCTTGCTAAGCGGATATTATTTGGCGGCACGAGAACTAGAATTTCTGGCTGGAAGGAAAGCAAATGGTTCCAATACCGATGGCCTTGCAGATGCTTTAGGAATCGCACAGCACCATGATGCAGTCACTGGCACTGCCAAACAACATACTACAAATGACTACGCAAAGCGCCTTTTCATCGGAGCTTCTGAGGCAGAAGCTGTTGTCAATTCCGATCTTTTGTGTCTAACTACAAATAAAACAGGAGATCAATGTACTAACTCAACACCGACTTTTAGCCAGTGTCAATTACTCAATATCAGCTACTGCCCTCCAACAGAGGAAGATATTCCACAAGGGAAGAGCTTGGTCGTTGTGGCATATAATCCTCTCGGATGGAATCGGACTGAGACTGTTCGGATTCCTGTTAACGATGCCACTCTTTCTGTTCAAGATTCTTCGGGAAATATTCTTGTGGTACAGTATGTAAGTTTGGACAATGTAACAGCGAATTTAAGAAACTTCTATGTAAAAGCCTACTTGGGACAGCCACCAGACCAAGTTCCAAAGTACTGGCTAATCTTTCAAGTCTCCGTGCCGCCACTTGGTTGGAATACATACTTCGTTTCAAGAACAGCTACCAAACGTAGATTGAAAAGCGGAAATGGATTTCTCTCTGCGATGGACAGTCCACAGAATGAGACAATTGAAATCGGACCTGGAGATCTGAAAATGTCGTTTTCTTTAGCTTCGGGACAACTCAATCGGATGTATAATTCTAAAACAGGAGTTGATGTCCCGATACAGCAGAGCTACCTCTGGTATGGTTCAAGCACAGGAGATGCCGATTCCCAGCAAGCTTCTGGTGCATACATATTCCGGCCTAATGGTGCCCCTCCAACTGTCGTCTCAAGATCAGTTCCTTTGAAGGTCTTTCGTGGACCGATAGTTGATGAGATTCACCAACAGTTCAGTCCATGGATCTATCAGGTTACTAGACTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACGGTCGGTCCTATTCCCACAGACGATGGTGTTGGAAAGGAGGTGATCACAAGAATGACAGCAAACATGGACACAAACAAGGAGTTCTACACCGATTCTAACGGAAGGGATTTCATTAAACGGGTACGAGATTATAGACCAGACTGGCCCCTTGTGGTTAATCAACCTGTGGCAGGAAACTATTATCCACTAAATCTTGGAATGTTTACTTCGGATAACAAATCCGAGTTCTCAGTCTTGGTAGATCGTGCCACTGGAGGATCAAGCATTGAAAATGGACAAATAGAGCTGATGCTTCACAGGCGTATACTTGAGGATGATTCGAGAGGAGTGGGTGAAGCACTTGATGAAACAGTGTGCATCGAAGAAAATACATGCGAAGGACTAACGGTTCGAGGGAACTATTATATGAGCGTAAACCAGGCAGGGTCGGGTGCCCCCTGGCGCCGCACAACTGGCCAGGAAGTTTACTCTCCTCTTCTTCTAGCATTCGCCCACGAGAAAATGGAGGATTGGGCTGCATCACATTTGACAAAATCATCTACCATGGATTCTAATTACAGCTTGCCTCTCAATGTTGCTTTGATTACTCTTCAGGAACTGGATGACGGTACTGTGCTCCTTCGGTTAGCTCATCTATATGAGGCAGCCGAAGATCCTCAATATTCAACACTGGCCAAAGTTGAACTGAAGAAGATGTTTACAGGAAAAACGATAAAAGAGGTGAAGGAAGTGAGCTTGTCTGCAAACCAAGAGAAGTCGGAGATGAAGAAGATGACATGGAAAGTCGAGGGTGACGGGGGAGATGAACCTGCACCGGTTAGAGGGGGGCCTGTCGATAGATCAGCTCTTGTTGTTGAGCTTGGACCCATGGAGATCCGCACCTTGGTGTTGAAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009987 GO:0015923 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1017

Amino Acids

113.49

Weight (kDa)

5.4

Isoelectric Point (pI)

37.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	46 - 357	4.2e-81	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	362 - 451	6.6e-17	Alpha mannosidase middle domain
Laman-like_dom	PF21260	557 - 580	1.2e-07	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	612 - 825	2.8e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	908 - 1012	7.2e-09	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66150 AT5G66150 AT5G66150
fragaria_vesca	FvH4_1g11550 FvH4_1g11600 FvH4_1g11600 FvH4_1g11600
malus_domestica	MD02G1128700.v1.1 MD02G1129000.v1.1
prunus_persica	Prupe.7G170500_v2.0.a1 Prupe.7G170500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0099191 RchiOBHm_Chr2g0099201
rosa_laevigata	RLG00000016854 RLG00000016860
rosa_multiflora	Rmu_co8341649.1_g000001 Rmu_sc0002025.1_g000008 Rmu_sc0010993.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143340 Rroxscaffold_2G00143370
rosa_rugosa	Rorug02G0077600 Rorug02G0078100 Rorug02G0078100
rosa_samantha	Rh2AG126500 Rh2AG126900 Rh2BG129700 Rh2BG129900 Rh2CG130700 Rh2CG131100 Rh2DG131100 Rh2DG131500
rosa_wichuraiana	Rw2G009780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2968
AccB1I	GGYRCC	3 cut(s)	2003, 2582, 2592
AccI	GTMKAC	1 cut(s)	2455
AccIII	TCCGGA	1 cut(s)	343
AcoI	YGGCCR	3 cut(s)	1037, 2605, 2823
AcsI	RAATTY	4 cut(s)	348, 1175, 1624, 3044
AcuI	CTGAAG	2 cut(s)	2726, 2858
AcyI	GRCGYC	1 cut(s)	2593
AdeI	CACNNNGTG	1 cut(s)	509
AfaI	GTAC	7 cut(s)	197, 905, 1390, 1593, 1685, 2274, 2761
AfeI	AGCGCT	1 cut(s)	835
AfiI	CCNNNNNNNGG	6 cut(s)	512, 734, 1718, 2321, 2972, 3034
AgeI	ACCGGT	1 cut(s)	2965
AhdI	GACNNNNNGTC	1 cut(s)	468
AhlI	ACTAGT	2 cut(s)	370, 1106
AjnI	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
AjuI	GAANNNNNNNTTGG	2 cut(s)	128, 160
AleI	CACNNNNGTG	1 cut(s)	3035
AloI	GAACNNNNNNTCC	2 cut(s)	2523, 2555
Alw21I	GWGCWC	2 cut(s)	576, 2769
Alw26I	GTCTC	7 cut(s)	98, 648, 1517, 1708, 1808, 2026, 2115
AlwNI	CAGNNNCTG	4 cut(s)	1262, 1334, 1440, 2749
Ama87I	CYCGRG	2 cut(s)	575, 1114
Aor13HI	TCCGGA	1 cut(s)	343
Aor51HI	AGCGCT	1 cut(s)	835
AoxI	GGCC	7 cut(s)	1037, 1216, 1994, 2294, 2605, 2823, 2978
ApeKI	GCWGC	3 cut(s)	1022, 2666, 2795
ApoI	RAATTY	4 cut(s)	348, 1175, 1624, 3044
ArsI	GACNNNNNNTTYG	2 cut(s)	2523, 2555
AsiGI	ACCGGT	1 cut(s)	2965
Asp700I	GAANNNNTTC	1 cut(s)	1461
AspLEI	GCGC	6 cut(s)	154, 490, 630, 836, 1305, 2595
AspS9I	GGNCC	6 cut(s)	1829, 2054, 2156, 2295, 2978, 3014
AsuC2I	CCSGG	3 cut(s)	735, 1115, 1116
AsuHPI	GGTGA	8 cut(s)	464, 556, 835, 2066, 2203, 2495, 2884, 2954
AsuII	TTCGAA	2 cut(s)	747, 765
AvaI	CYCGRG	2 cut(s)	575, 1114
AvaII	GGWCC	4 cut(s)	1829, 2054, 2156, 3014
BaeGI	GKGCMC	2 cut(s)	2008, 2587
BalI	TGGCCA	3 cut(s)	1039, 2607, 2825
BamHI	GGATCC	1 cut(s)	665
BanI	GGYRCC	3 cut(s)	2003, 2582, 2592
BanII	GRGCYC	1 cut(s)	576
BauI	CACGAG	4 cut(s)	359, 507, 1164, 2645
BbsI	GAAGAC	1 cut(s)	188
Bbv12I	GWGCWC	2 cut(s)	576, 2769
BbvI	GCAGC	3 cut(s)	1034, 2653, 2807
BccI	CCATC	9 cut(s)	301, 318, 510, 880, 1003, 1208, 1503, 1789, 2168
BceAI	ACGGC	1 cut(s)	530
BcgI	CGANNNNNNTGC	2 cut(s)	1202, 1236
BciT130I	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BclI	TGATCA	3 cut(s)	190, 454, 2193
BcnI	CCSGG	3 cut(s)	735, 1115, 1116
BcoDI	GTCTC	7 cut(s)	98, 648, 1517, 1708, 1808, 2026, 2115
BcuI	ACTAGT	2 cut(s)	370, 1106
BfaI	CTAG	7 cut(s)	371, 467, 647, 1107, 1172, 2109, 2633
BfmI	CTRYAG	1 cut(s)	721
BfoI	RGCGCY	3 cut(s)	837, 1306, 2596
BfuAI	ACCTGC	1 cut(s)	2968
BglII	AGATCT	1 cut(s)	1837
BisI	GCNGC	7 cut(s)	85, 1023, 1161, 1712, 2596, 2667, 2796
BlpI	GCTNAGC	2 cut(s)	570, 1142
BlsI	GCNGC	7 cut(s)	86, 1024, 1162, 1713, 2597, 2668, 2797
BmcAI	AGTACT	2 cut(s)	197, 1685
Bme1390I	CCNGG	8 cut(s)	735, 776, 1115, 1116, 1833, 2570, 2590, 2609
Bme18I	GGWCC	4 cut(s)	1829, 2054, 2156, 3014
BmeRI	GACNNNNNGTC	1 cut(s)	468
BmeT110I	CYCGRG	2 cut(s)	575, 1114
BmgT120I	GGNCC	6 cut(s)	1829, 2054, 2156, 2295, 2978, 3014
BmiI	GGNNCC	8 cut(s)	667, 1204, 2005, 2297, 2584, 2594, 2979, 3016
BmrFI	CCNGG	8 cut(s)	735, 776, 1115, 1116, 1833, 2570, 2590, 2609
BpiI	GAAGAC	1 cut(s)	188
BpmI	CTGGAG	2 cut(s)	1854, 2424
Bpu1102I	GCTNAGC	2 cut(s)	570, 1142
Bpu14I	TTCGAA	2 cut(s)	747, 765
BpuEI	CTTGAG	2 cut(s)	2008, 2480
BpuMI	CCSGG	3 cut(s)	735, 1115, 1116
Bsa29I	ATCGAT	1 cut(s)	606
BsaHI	GRCGYC	1 cut(s)	2593
BsaJI	CCNNGG	6 cut(s)	1114, 2090, 2588, 2694, 3018, 3033
BsaWI	WCCGGW	3 cut(s)	343, 712, 2965
BsaXI	ACNNNNNCTCC	2 cut(s)	1893, 1923
Bsc4I	CCNNNNNNNGG	6 cut(s)	512, 734, 1718, 2321, 2972, 3034
Bse118I	RCCGGY	1 cut(s)	2965
BseAI	TCCGGA	1 cut(s)	343
BseBI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BseCI	ATCGAT	1 cut(s)	606
BseDI	CCNNGG	6 cut(s)	1114, 2090, 2588, 2694, 3018, 3033
BseGI	GGATG	5 cut(s)	521, 1514, 1887, 2470, 2758
BseLI	CCNNNNNNNGG	6 cut(s)	512, 734, 1718, 2321, 2972, 3034
BseMII	CTCAG	7 cut(s)	294, 561, 704, 1041, 1314, 1512, 2394
BseRI	GAGGAG	2 cut(s)	2490, 2613
BseSI	GKGCMC	2 cut(s)	2008, 2587
BseXI	GCAGC	3 cut(s)	1034, 2653, 2807
BseYI	CCCAGC	2 cut(s)	380, 1965
BsgI	GTGCAG	1 cut(s)	2946
Bsh1285I	CGRYCG	2 cut(s)	970, 2154
BshFI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BshNI	GGYRCC	3 cut(s)	2003, 2582, 2592
BshTI	ACCGGT	1 cut(s)	2965
BshVI	ATCGAT	1 cut(s)	606
BsiEI	CGRYCG	2 cut(s)	970, 2154
BsiHKAI	GWGCWC	2 cut(s)	576, 2769
BsiHKCI	CYCGRG	2 cut(s)	575, 1114
BsiSI	CCGG	6 cut(s)	344, 713, 735, 1115, 1993, 2966
BslFI	GGGAC	3 cut(s)	1671, 1881, 1895
BslI	CCNNNNNNNGG	6 cut(s)	512, 734, 1718, 2321, 2972, 3034
BsmAI	GTCTC	7 cut(s)	98, 648, 1517, 1708, 1808, 2026, 2115
BsmBI	CGTCTC	2 cut(s)	98, 2026
BsmFI	GGGAC	3 cut(s)	1671, 1881, 1895
BsmI	GAATGC	4 cut(s)	154, 219, 311, 2636
BsnI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BsoBI	CYCGRG	2 cut(s)	575, 1114
Bsp119I	TTCGAA	2 cut(s)	747, 765
Bsp1286I	GDGCHC	4 cut(s)	576, 2008, 2587, 2769
Bsp13I	TCCGGA	1 cut(s)	343
Bsp1720I	GCTNAGC	2 cut(s)	570, 1142
Bsp19I	CCATGG	3 cut(s)	2090, 2694, 3018
BspANI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BspCNI	CTCAG	7 cut(s)	295, 562, 703, 1042, 1315, 1513, 2393
BspDI	ATCGAT	1 cut(s)	606
BspEI	TCCGGA	1 cut(s)	343
BspLI	GGNNCC	8 cut(s)	667, 1204, 2005, 2297, 2584, 2594, 2979, 3016
BspMI	ACCTGC	1 cut(s)	2968
BspQI	GCTCTTC	1 cut(s)	1469
BspT104I	TTCGAA	2 cut(s)	747, 765
BspT107I	GGYRCC	3 cut(s)	2003, 2582, 2592
BsrFI	RCCGGY	1 cut(s)	2965
BssAI	RCCGGY	1 cut(s)	2965
BssECI	CCNNGG	6 cut(s)	1114, 2090, 2588, 2694, 3018, 3033
BssNAI	GTATAC	1 cut(s)	2456
BssNI	GRCGYC	1 cut(s)	2593
BssSI	CACGAG	4 cut(s)	359, 507, 1164, 2645
BssT1I	CCWWGG	4 cut(s)	2090, 2694, 3018, 3033
Bst1107I	GTATAC	1 cut(s)	2456
Bst2BI	CACGAG	4 cut(s)	359, 507, 1164, 2645
Bst2UI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
Bst6I	CTCTTC	4 cut(s)	792, 1469, 2631, 2745
BstACI	GRCGYC	1 cut(s)	2593
BstBI	TTCGAA	2 cut(s)	747, 765
BstC8I	GCNNGC	5 cut(s)	552, 1139, 1971, 2133, 2714
BstDEI	CTNAG	8 cut(s)	303, 570, 690, 1050, 1142, 1323, 1521, 2380
BstDSI	CCRYGG	3 cut(s)	2090, 2694, 3018
BstEII	GGTNACC	1 cut(s)	470
BstENI	CCTNNNNNAGG	1 cut(s)	2319
BstF5I	GGATG	5 cut(s)	521, 1514, 1887, 2470, 2758
BstH2I	RGCGCY	3 cut(s)	837, 1306, 2596
BstHHI	GCGC	6 cut(s)	154, 490, 630, 836, 1305, 2595
BstMAI	GTCTC	7 cut(s)	98, 648, 1517, 1708, 1808, 2026, 2115
BstMCI	CGRYCG	2 cut(s)	970, 2154
BstNI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
BstNSI	RCATGY	2 cut(s)	2135, 2526
BstPI	GGTNACC	1 cut(s)	470
BstSCI	CCNGG	8 cut(s)	733, 774, 1113, 1114, 1831, 2568, 2588, 2607
BstSFI	CTRYAG	1 cut(s)	721
BstSLI	GKGCMC	2 cut(s)	2008, 2587
BstV1I	GCAGC	3 cut(s)	1034, 2653, 2807
BstV2I	GAAGAC	1 cut(s)	188
BstX2I	RGATCY	5 cut(s)	665, 1837, 2094, 2803, 3024
BstXI	CCANNNNNNTGG	2 cut(s)	1686, 1722
BstYI	RGATCY	5 cut(s)	665, 1837, 2094, 2803, 3024
BstZ17I	GTATAC	1 cut(s)	2456
Bsu15I	ATCGAT	1 cut(s)	606
BsuRI	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
BsuTUI	ATCGAT	1 cut(s)	606
BtgI	CCRYGG	3 cut(s)	2090, 2694, 3018
BtgZI	GCGATG	1 cut(s)	1808
BtsCI	GGATG	5 cut(s)	521, 1514, 1887, 2470, 2758
BtsI	GCAGTG	1 cut(s)	1266
BtsIMutI	CAGTG	7 cut(s)	1040, 1260, 1266, 1421, 2400, 2508, 2819
BveI	ACCTGC	1 cut(s)	2968
Cac8I	GCNNGC	5 cut(s)	552, 1139, 1971, 2133, 2714
CaiI	CAGNNNCTG	4 cut(s)	1262, 1334, 1440, 2749
CfoI	GCGC	6 cut(s)	154, 490, 630, 836, 1305, 2595
Cfr10I	RCCGGY	1 cut(s)	2965
Cfr13I	GGNCC	6 cut(s)	1829, 2054, 2156, 2295, 2978, 3014
Cfr9I	CCCGGG	1 cut(s)	1114
ClaI	ATCGAT	1 cut(s)	606
CseI	GACGC	1 cut(s)	472
Csp6I	GTAC	7 cut(s)	196, 904, 1389, 1592, 1684, 2273, 2760
CspAI	ACCGGT	1 cut(s)	2965
CspCI	CAANNNNNGTGG	2 cut(s)	308, 343
CviQI	GTAC	7 cut(s)	196, 904, 1389, 1592, 1684, 2273, 2760
DdeI	CTNAG	8 cut(s)	303, 570, 690, 1050, 1142, 1323, 1521, 2380
DinI	GGCGCC	1 cut(s)	2594
DraIII	CACNNNGTG	1 cut(s)	509
DriI	GACNNNNNGTC	1 cut(s)	468
EaeI	YGGCCR	3 cut(s)	1037, 2605, 2823
Eam1104I	CTCTTC	4 cut(s)	792, 1469, 2631, 2745
Eam1105I	GACNNNNNGTC	1 cut(s)	468
EarI	CTCTTC	4 cut(s)	792, 1469, 2631, 2745
EciI	GGCGGA	1 cut(s)	26
Ecl136II	GAGCTC	1 cut(s)	574
Eco130I	CCWWGG	4 cut(s)	2090, 2694, 3018, 3033
Eco24I	GRGCYC	1 cut(s)	576
Eco47I	GGWCC	4 cut(s)	1829, 2054, 2156, 3014
Eco47III	AGCGCT	1 cut(s)	835
Eco53kI	GAGCTC	1 cut(s)	574
Eco57I	CTGAAG	2 cut(s)	2726, 2858
Eco88I	CYCGRG	2 cut(s)	575, 1114
Eco91I	GGTNACC	1 cut(s)	470
EcoICRI	GAGCTC	1 cut(s)	574
EcoNI	CCTNNNNNAGG	1 cut(s)	2319
EcoO109I	RGGNCCY	1 cut(s)	2978
EcoO65I	GGTNACC	1 cut(s)	470
EcoRII	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
EcoT14I	CCWWGG	4 cut(s)	2090, 2694, 3018, 3033
EcoT22I	ATGCAT	1 cut(s)	420
EcoT38I	GRGCYC	1 cut(s)	576
EgeI	GGCGCC	1 cut(s)	2594
EheI	GGCGCC	1 cut(s)	2594
ErhI	CCWWGG	4 cut(s)	2090, 2694, 3018, 3033
Esp3I	CGTCTC	2 cut(s)	98, 2026
FaqI	GGGAC	3 cut(s)	1671, 1881, 1895
FbaI	TGATCA	3 cut(s)	190, 454, 2193
FblI	GTMKAC	1 cut(s)	2455
Fnu4HI	GCNGC	7 cut(s)	85, 1023, 1161, 1712, 2596, 2667, 2796
FokI	GGATG	5 cut(s)	528, 1521, 1894, 2477, 2765
FriOI	GRGCYC	1 cut(s)	576
Fsp4HI	GCNGC	7 cut(s)	85, 1023, 1161, 1712, 2596, 2667, 2796
FspBI	CTAG	7 cut(s)	371, 467, 647, 1107, 1172, 2109, 2633
GlaI	GCGC	6 cut(s)	153, 489, 629, 835, 1304, 2594
GluI	GCNGC	7 cut(s)	85, 1023, 1161, 1712, 2596, 2667, 2796
GsaI	CCCAGC	2 cut(s)	384, 1969
GsuI	CTGGAG	2 cut(s)	1854, 2424
HaeII	RGCGCY	3 cut(s)	837, 1306, 2596
HaeIII	GGCC	7 cut(s)	1039, 1218, 1996, 2296, 2607, 2825, 2980
HapII	CCGG	6 cut(s)	344, 713, 735, 1115, 1993, 2966
HgaI	GACGC	1 cut(s)	472
HhaI	GCGC	6 cut(s)	154, 490, 630, 836, 1305, 2595
Hin1I	GRCGYC	1 cut(s)	2593
Hin6I	GCGC	6 cut(s)	152, 488, 628, 834, 1303, 2593
HinP1I	GCGC	6 cut(s)	152, 488, 628, 834, 1303, 2593
HincII	GTYRAC	2 cut(s)	333, 1543
HindII	GTYRAC	2 cut(s)	333, 1543
HindIII	AAGCTT	2 cut(s)	552, 1971
HpaI	GTTAAC	2 cut(s)	333, 1543
HpaII	CCGG	6 cut(s)	344, 713, 735, 1115, 1993, 2966
HphI	GGTGA	8 cut(s)	464, 556, 835, 2066, 2203, 2495, 2884, 2954
Hpy99I	CGWCG	1 cut(s)	466
HpyAV	CCTTC	7 cut(s)	1117, 1182, 2035, 2247, 2522, 2780, 2869
HpyCH4IV	ACGT	2 cut(s)	245, 1759
HpyF3I	CTNAG	8 cut(s)	303, 570, 690, 1050, 1142, 1323, 1521, 2380
HpySE526I	ACGT	2 cut(s)	245, 1759
Hsp92I	GRCGYC	1 cut(s)	2593
HspAI	GCGC	6 cut(s)	152, 488, 628, 834, 1303, 2593
KasI	GGCGCC	1 cut(s)	2592
Kpn2I	TCCGGA	1 cut(s)	343
Ksp22I	TGATCA	3 cut(s)	190, 454, 2193
KspAI	GTTAAC	2 cut(s)	333, 1543
LguI	GCTCTTC	1 cut(s)	1469
LmnI	GCTCC	2 cut(s)	1316, 2772
Lsp1109I	GCAGC	3 cut(s)	1034, 2653, 2807
MaeI	CTAG	7 cut(s)	371, 467, 647, 1107, 1172, 2109, 2633
MaeII	ACGT	2 cut(s)	245, 1759
MaeIII	GTNAC	6 cut(s)	470, 856, 1258, 1615, 2104, 2942
MfeI	CAATTG	1 cut(s)	1818
MflI	RGATCY	5 cut(s)	665, 1837, 2094, 2803, 3024
MhlI	GDGCHC	4 cut(s)	576, 2008, 2587, 2769
MlsI	TGGCCA	3 cut(s)	1039, 2607, 2825
MluNI	TGGCCA	3 cut(s)	1039, 2607, 2825
Mly113I	GGCGCC	1 cut(s)	2593
MmeI	TCCRAC	7 cut(s)	184, 228, 1472, 1703, 2036, 2161, 2889
Mox20I	TGGCCA	3 cut(s)	1039, 2607, 2825
Mph1103I	ATGCAT	1 cut(s)	420
MroI	TCCGGA	1 cut(s)	343
MroXI	GAANNNNTTC	1 cut(s)	1461
MscI	TGGCCA	3 cut(s)	1039, 2607, 2825
MseI	TTAA	8 cut(s)	332, 944, 957, 975, 1542, 1628, 2265, 2307
MslI	CAYNNNNRTG	6 cut(s)	165, 446, 1251, 2201, 2787, 3035
Msp20I	TGGCCA	3 cut(s)	1039, 2607, 2825
MspA1I	CMGCKG	1 cut(s)	569
MspI	CCGG	6 cut(s)	344, 713, 735, 1115, 1993, 2966
MspR9I	CCNGG	8 cut(s)	735, 776, 1115, 1116, 1833, 2570, 2590, 2609
MunI	CAATTG	1 cut(s)	1818
Mva1269I	GAATGC	4 cut(s)	154, 219, 311, 2636
MvaI	CCWGG	5 cut(s)	776, 1833, 2570, 2590, 2609
NarI	GGCGCC	1 cut(s)	2593
NciI	CCSGG	3 cut(s)	735, 1115, 1116
NcoI	CCATGG	3 cut(s)	2090, 2694, 3018
NlaIV	GGNNCC	8 cut(s)	667, 1204, 2005, 2297, 2584, 2594, 2979, 3016
NmuCI	GTSAC	4 cut(s)	470, 856, 1258, 2942
NsiI	ATGCAT	1 cut(s)	420
NspI	RCATGY	2 cut(s)	2135, 2526
NspV	TTCGAA	2 cut(s)	747, 765
OliI	CACNNNNGTG	1 cut(s)	3035
PaeI	GCATGC	1 cut(s)	2135
PciSI	GCTCTTC	1 cut(s)	1469
PctI	GAATGC	4 cut(s)	154, 219, 311, 2636
PdmI	GAANNNNTTC	1 cut(s)	1461
PfoI	TCCNGGA	1 cut(s)	774
PinAI	ACCGGT	1 cut(s)	2965
PkrI	GCNGC	7 cut(s)	86, 1024, 1162, 1713, 2597, 2668, 2797
PluTI	GGCGCC	1 cut(s)	2596
Psp124BI	GAGCTC	1 cut(s)	576
Psp6I	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
PspEI	GGTNACC	1 cut(s)	470
PspFI	CCCAGC	2 cut(s)	380, 1965
PspGI	CCWGG	5 cut(s)	774, 1831, 2568, 2588, 2607
PspN4I	GGNNCC	8 cut(s)	667, 1204, 2005, 2297, 2584, 2594, 2979, 3016
PspPI	GGNCC	6 cut(s)	1829, 2054, 2156, 2295, 2978, 3014
PstNI	CAGNNNCTG	4 cut(s)	1262, 1334, 1440, 2749
PsuI	RGATCY	5 cut(s)	665, 1837, 2094, 2803, 3024
RsaI	GTAC	7 cut(s)	197, 905, 1390, 1593, 1685, 2274, 2761
RsaNI	GTAC	7 cut(s)	196, 904, 1389, 1592, 1684, 2273, 2760
RseI	CAYNNNNRTG	6 cut(s)	165, 446, 1251, 2201, 2787, 3035
SacI	GAGCTC	1 cut(s)	576
SapI	GCTCTTC	1 cut(s)	1469
SaqAI	TTAA	8 cut(s)	332, 944, 957, 975, 1542, 1628, 2265, 2307
SatI	GCNGC	7 cut(s)	85, 1023, 1161, 1712, 2596, 2667, 2796
Sau96I	GGNCC	6 cut(s)	1829, 2054, 2156, 2295, 2978, 3014
ScaI	AGTACT	2 cut(s)	197, 1685
ScrFI	CCNGG	8 cut(s)	735, 776, 1115, 1116, 1833, 2570, 2590, 2609
SduI	GDGCHC	4 cut(s)	576, 2008, 2587, 2769
SfcI	CTRYAG	1 cut(s)	721
SfoI	GGCGCC	1 cut(s)	2594
SfuI	TTCGAA	2 cut(s)	747, 765
SinI	GGWCC	4 cut(s)	1829, 2054, 2156, 3014
SmaI	CCCGGG	1 cut(s)	1116
SmiMI	CAYNNNNRTG	6 cut(s)	165, 446, 1251, 2201, 2787, 3035
SmlI	CTYRAG	2 cut(s)	2023, 2459
SmoI	CTYRAG	2 cut(s)	2023, 2459
SpeI	ACTAGT	2 cut(s)	370, 1106
SphI	GCATGC	1 cut(s)	2135
SspDI	GGCGCC	1 cut(s)	2592
SspI	AATATT	2 cut(s)	1582, 2813
SspMI	CTAG	7 cut(s)	371, 467, 647, 1107, 1172, 2109, 2633
SstI	GAGCTC	1 cut(s)	576
StyD4I	CCNGG	8 cut(s)	733, 774, 1113, 1114, 1831, 2568, 2588, 2607
StyI	CCWWGG	4 cut(s)	2090, 2694, 3018, 3033
TaiI	ACGT	2 cut(s)	248, 1762
TaqII	GACCGA	2 cut(s)	2071, 2144
TatI	WGTACW	3 cut(s)	195, 1388, 1683
TauI	GCSGC	4 cut(s)	87, 1163, 1714, 2598
Tru1I	TTAA	8 cut(s)	332, 944, 957, 975, 1542, 1628, 2265, 2307
Tru9I	TTAA	8 cut(s)	332, 944, 957, 975, 1542, 1628, 2265, 2307
TscAI	CASTG	7 cut(s)	1047, 1267, 1273, 1421, 2407, 2508, 2826
TseFI	GTSAC	4 cut(s)	470, 856, 1258, 2942
TseI	GCWGC	3 cut(s)	1022, 2666, 2795
Tsp45I	GTSAC	4 cut(s)	470, 856, 1258, 2942
TspDTI	ATGAA	9 cut(s)	131, 438, 675, 817, 1300, 2251, 2511, 2930, 2970
TspGWI	ACGGA	3 cut(s)	1053, 1696, 2265
TspMI	CCCGGG	1 cut(s)	1114
TspRI	CASTG	7 cut(s)	1047, 1267, 1273, 1421, 2407, 2508, 2826
VpaK11BI	GGWCC	4 cut(s)	1829, 2054, 2156, 3014
XagI	CCTNNNNNAGG	1 cut(s)	2319
XapI	RAATTY	4 cut(s)	348, 1175, 1624, 3044
XbaI	TCTAGA	1 cut(s)	646
XceI	RCATGY	2 cut(s)	2135, 2526
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1973
XmaI	CCCGGG	1 cut(s)	1114
XmiI	GTMKAC	1 cut(s)	2455
XmnI	GAANNNNTTC	1 cut(s)	1461
XspI	CTAG	7 cut(s)	371, 467, 647, 1107, 1172, 2109, 2633
ZrmI	AGTACT	2 cut(s)	197, 1685
Zsp2I	ATGCAT	1 cut(s)	420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.