Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004264

Physical Location & Seq

Genomic Coordinates Reverse (-)

10740997 .. 10748946

Gene Structure 7950 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016860

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGCTAAAGCTCGCGGCGTTTTTCGAGCTTGTGATTGTGGTTGTTTGGTTGGGAGTGGCGAGTGTTGATGGAGTTTTTGTGAAGTACCAGACCGGGAGTGGTGTTGTGGAAGGGAAGTTGAATGTTCATTTGGTTGCGCATTCCCATGATGACGTTGGTTGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAATAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTCGTTGAATCGTTGGTTCGTGATCCGAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTAACACAGAGTGTGGAAGTTCAAGAACGAGTGCGGAAACTAGTAGATGCTGGCCAGTTAGAGTTTGTAAATGGAGGTTGGTGCATGCATGATGAGGCTGCAGTCCATTACATAGACATGATTGACCAAACAACTCTAGGCCACCTTGCTATAAAGCAGCAGTTCAACAAGACACCTCGAGCGGGGTGGCAAATTGATCCGTTCGGACACTCTGCTGTGCAGGGTTACTTACTTGGAGCTGAGCTTGGATTCGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGCACAGCGTAAGGGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTCGTCACTATAGTCCGCCAGATGGTTTCCATTTTGAAGTTTTTGATGACTTTGTCCCTGTGCAGGATAATCCGCTTATTTTTGACTACAATGTTGAACTGCGCGTTAATGATTTTATTACTGCTGCAATCACCCAAGCAAATGTGACTAGGGGAAATCACATGATGTGGACAATGGGAGATGATTTCCAGTACCAATATGCTGAATCTTGGTTCAAACAAATGGATAAGTTAATTCACTATGTTAATAAGGATGGCCGAGTTAATGCCTTATATTCTACACCGTCTCTCTACACTGATGCTAAAAATGCAGCAAATCAATCATGGCCGTTAAAAACTGAGGATTATTTTCCGTATGCAGATATAATAAATGCTTATTGGACCGGTTATTTTACTAGTCGCCCAGGTTTGAAAAGATATATTCGATCACTGAGCGGATATTATTTGGCAGCAAGAGAACTAGAATTTCTGGTTGGAAAGAGAGCGAATGGTTCAAGTACCTATTCCCTTGGAGATGCTTTAGGAATTGCACAGCACCACGATGCTGCCACTGGCACTGCTAAACAGCATACAACAAATGACTATGTAAAGCGCCTGTCAATTGGTGTCACTGAGGCAGAAGCTGTTGTCAGTTCTGCTCTGTCGTGCCTAACTACAAATAAATCTGGAGATCAATGTAAAGACCCAGCAGTTACTTTTAGCCAGTGTCGACTACTCAATATCAGTTTCTGTCCTCCAACGGAGGAAGATATTCCAGAAGGGAAGAGCTTGGTTGTTGTGGCATATAATCCTCTTGGATGGAATCGCACTGAGATTGTTAGGATACCTGTTAATTATGATGATCTTGTTGTCCAAGATTCCTCCGGAAACATCCTCGAGACACAATATGTGAGTGTGGATAATGTTACAAGCAATCTAAGAACATTTTATACAGAAGCCTACTTGGGACAGTCATCAAAACAAATTCCCAAGTACTGGCTAATCTTTCAAGCCTCTGTGTCACCACTTGGTTGGGACACATACTTCATCTCTAGAGGAGCTACCAAAGGGAAAAGAAGAGTTGGACTTCTCTCTGTGAAGGACAGTCCACAGAATGAGACCATTGAAATTGGACCAGGAGATCTGAAGATGTCCTTTTCTTCTGCTTCTGGACAACTCAAGCGGATGTATAATTCTAAAACAGGAGTTGATGTACCAATACAGCAGAGCTACCTCTGGTATGGTTCAAGCACCGATGGCCAGGCTTCAGGTGCATATATATTCCGGCCTAATGGTGCCCCTCCAACTATTGTGTCAAGATCTGTACCCTTGAAGGTCTTTCGTGGACCATTATTCGATGAGATTCACCAAGAATTCAGTTCATGGATCTATCAGGTCATTAGAGTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACTATTGGTCCAATTCCTACAGACGACGGTGTTGGTAAAGAGGTCATCACACGAATGACAGCAAACATGGAGACAAACAAGATGTTTTACACTGATTCCAATGGGAGAGATTTCATTAAAAGGGTACGAGATTATAGAGCAGACTGGAACCTTTCAGTTAATCAACCTGTTGCAGGCAACTACTATCCACTAAACCTTGGGATCTTTACTTCGGATAACAAATCTGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCCAGCATTCAAGACGGACAAGTAGAGATGATGCTACATAGGCGCCTACTGGTTGATGATGGTAGAGGGGTGGGTGAAGCCCTTGATGAAACAGTGTGCATCAAGAATAATACATGTCAAGGACTAACGGTTCGAGGAAACTATTATATGAGCATAAACCAGTTCGGGGATGGTGCCCAATGGCGTCGGAAAACTGGACAAGAAGTTTACTCGCCACTTCTTCTAGCTTTCACCCATGAGAACTTGGAGAATTGGACTGCATCTCATTCAACGAAAGGGTCTGTCATGGATTCAAGTTACAGCTTGCCTCCCAATGTTGCTTTGATTACTCTTCAGGAGTTGGATGGTGGAAGTGTACTTCTCCGTCTAGCACATCTATATGAGGCTGCTGAAGCTCCTCAATATTCTACATTGGCAAAAGTTGAACTGAAGAAGATGCTAACAGGAAAAACGATAAAGCAGTTGAAAGAAGTGAGCTTGTCAGCAAACCAAGAGAAGTCAGCGATGAAGAAGATGACGTGGAAAGTCGAGGGTTCCACGGGAGAAGAACCTACACCAGTAAAAGGCGGCGCTGTCGATAGTTCAACTCTTGTTGTTGAGCTTGGACCCATGGAGATACGAGCGGCGCGTTTCCATGGCGTGGCAGGAATGGCGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009987 GO:0015923 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1012

Amino Acids

112.99

Weight (kDa)

5.99

Isoelectric Point (pI)

35.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	41 - 352	1e-77	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	357 - 449	5.1e-18	Alpha mannosidase middle domain
Laman-like_dom	PF21260	539 - 575	9.3e-06	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	605 - 814	1.3e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	897 - 999	3.8e-06	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66150 AT5G66150 AT5G66150
fragaria_vesca	FvH4_1g11550 FvH4_1g11600 FvH4_1g11600 FvH4_1g11600
malus_domestica	MD02G1128700.v1.1 MD02G1129000.v1.1
prunus_persica	Prupe.7G170500_v2.0.a1 Prupe.7G170500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0099191 RchiOBHm_Chr2g0099201
rosa_laevigata	RLG00000016854 RLG00000016860
rosa_multiflora	Rmu_co8341649.1_g000001 Rmu_sc0002025.1_g000008 Rmu_sc0010993.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143340 Rroxscaffold_2G00143370
rosa_rugosa	Rorug02G0077600 Rorug02G0078100 Rorug02G0078100
rosa_samantha	Rh2AG126500 Rh2AG126900 Rh2BG129700 Rh2BG129900 Rh2CG130700 Rh2CG131100 Rh2DG131100 Rh2DG131500
rosa_wichuraiana	Rw2G009780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	138
AccB1I	GGYRCC	3 cut(s)	1970, 2418, 2549
AccB7I	CCANNNNNTGG	2 cut(s)	1671, 3016
AccBSI	CCGCTC	3 cut(s)	497, 1131, 2999
AccI	GTMKAC	1 cut(s)	1405
AccII	CGCG	3 cut(s)	14, 801, 3004
AccIII	TCCGGA	1 cut(s)	1559
AciI	CCGC	9 cut(s)	14, 349, 497, 713, 770, 1131, 1858, 2943, 2999
AclWI	GGATC	9 cut(s)	199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AcoI	YGGCCR	4 cut(s)	367, 952, 1022, 1933
AcsI	RAATTY	3 cut(s)	1160, 1659, 2048
AcuI	CTGAAG	5 cut(s)	1841, 1926, 2693, 2787, 2825
AcyI	GRCGYC	2 cut(s)	2419, 2560
AdeI	CACNNNGTG	1 cut(s)	326
AfiI	CCNNNNNNNGG	8 cut(s)	497, 719, 760, 1671, 2288, 2542, 2939, 3016
AflIII	ACRYGT	1 cut(s)	2489
AgeI	ACCGGT	1 cut(s)	1079
AhlI	ACTAGT	2 cut(s)	355, 1091
AjiI	CACGTC	1 cut(s)	2895
AjnI	CCWGG	3 cut(s)	1099, 1810, 1935
AjuI	GAANNNNNNNTTGG	6 cut(s)	113, 145, 1152, 1184, 2103, 2135
AloI	GAACNNNNNNTCC	2 cut(s)	2490, 2522
Alw21I	GWGCWC	1 cut(s)	610
Alw26I	GTCTC	6 cut(s)	633, 987, 1568, 1787, 2082, 2180
AlwI	GGATC	9 cut(s)	199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AlwNI	CAGNNNCTG	2 cut(s)	1319, 1425
Ama87I	CYCGRG	2 cut(s)	492, 1571
Aor13HI	TCCGGA	1 cut(s)	1559
AoxI	GGCC	6 cut(s)	367, 454, 952, 1022, 1933, 1961
ApeKI	GCWGC	7 cut(s)	413, 472, 821, 1007, 1145, 1241, 2762
ApoI	RAATTY	3 cut(s)	1160, 1659, 2048
ArsI	GACNNNNNNTTYG	2 cut(s)	2490, 2522
AsiGI	ACCGGT	1 cut(s)	1079
Asp700I	GAANNNNTTC	1 cut(s)	1446
AspLEI	GCGC	6 cut(s)	139, 801, 1290, 2421, 2948, 3004
AspS9I	GGNCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
AsuC2I	CCSGG	1 cut(s)	94
AsuHPI	GGTGA	5 cut(s)	820, 1689, 2033, 2462, 2599
AvaI	CYCGRG	2 cut(s)	492, 1571
AvaII	GGWCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
BaeGI	GKGCMC	2 cut(s)	1975, 2554
BalI	TGGCCA	2 cut(s)	369, 1935
BamHI	GGATCC	2 cut(s)	650, 2375
BanI	GGYRCC	3 cut(s)	1970, 2418, 2549
BbsI	GAAGAC	1 cut(s)	173
Bbv12I	GWGCWC	1 cut(s)	610
BbvI	GCAGC	7 cut(s)	400, 484, 808, 1019, 1157, 1228, 2749
BceAI	ACGGC	1 cut(s)	1009
BciT130I	CCWGG	3 cut(s)	1101, 1812, 1937
BciVI	GTATCC	1 cut(s)	1512
BclI	TGATCA	1 cut(s)	175
BcnI	CCSGG	1 cut(s)	94
BcoDI	GTCTC	6 cut(s)	633, 987, 1568, 1787, 2082, 2180
BcuI	ACTAGT	2 cut(s)	355, 1091
BfaI	CTAG	8 cut(s)	356, 452, 846, 1092, 1157, 1728, 2600, 2744
BfmI	CTRYAG	3 cut(s)	414, 706, 2133
BfoI	RGCGCY	3 cut(s)	1291, 2422, 2949
BfuI	GTATCC	1 cut(s)	1512
BglII	AGATCT	2 cut(s)	1816, 1994
BlpI	GCTNAGC	1 cut(s)	555
BmcAI	AGTACT	2 cut(s)	182, 1670
Bme1390I	CCNGG	4 cut(s)	94, 1101, 1812, 1937
Bme18I	GGWCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
BmeT110I	CYCGRG	2 cut(s)	492, 1571
BmgBI	CACGTC	1 cut(s)	2895
BmgT120I	GGNCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
BmiI	GGNNCC	8 cut(s)	652, 1972, 2264, 2377, 2420, 2551, 2911, 2983
BmrFI	CCNGG	4 cut(s)	94, 1101, 1812, 1937
BmsI	GCATC	8 cut(s)	352, 985, 1201, 1228, 2397, 2484, 2645, 2802
BpiI	GAAGAC	1 cut(s)	173
BpmI	CTGGAG	2 cut(s)	1383, 2391
Bpu1102I	GCTNAGC	1 cut(s)	555
BpuEI	CTTGAG	1 cut(s)	1838
BpuMI	CCSGG	1 cut(s)	94
BsaHI	GRCGYC	2 cut(s)	2419, 2560
BsaI	GGTCTC	1 cut(s)	1787
BsaJI	CCNNGG	6 cut(s)	1099, 1204, 2311, 2913, 2985, 3010
BsaWI	WCCGGW	2 cut(s)	1079, 1559
BsaXI	ACNNNNNCTCC	4 cut(s)	63, 93, 1872, 1902
Bsc4I	CCNNNNNNNGG	8 cut(s)	497, 719, 760, 1671, 2288, 2542, 2939, 3016
Bse118I	RCCGGY	1 cut(s)	1079
BseAI	TCCGGA	1 cut(s)	1559
BseBI	CCWGG	3 cut(s)	1101, 1812, 1937
BseDI	CCNNGG	6 cut(s)	1099, 1204, 2311, 2913, 2985, 3010
BseGI	GGATG	6 cut(s)	955, 1499, 1566, 1866, 2551, 2725
BseLI	CCNNNNNNNGG	8 cut(s)	497, 719, 760, 1671, 2288, 2542, 2939, 3016
BseMII	CTCAG	9 cut(s)	279, 546, 689, 1026, 1118, 1299, 1497, 2331, 2361
BseRI	GAGGAG	2 cut(s)	1746, 2763
BseSI	GKGCMC	2 cut(s)	1975, 2554
BseXI	GCAGC	7 cut(s)	400, 484, 808, 1019, 1157, 1228, 2749
BseYI	CCCAGC	1 cut(s)	1381
BsgI	GTGCAG	2 cut(s)	554, 779
Bsh1236I	CGCG	3 cut(s)	14, 801, 3004
BshFI	GGCC	6 cut(s)	369, 456, 954, 1024, 1935, 1963
BshNI	GGYRCC	3 cut(s)	1970, 2418, 2549
BshTI	ACCGGT	1 cut(s)	1079
BsiHKAI	GWGCWC	1 cut(s)	610
BsiHKCI	CYCGRG	2 cut(s)	492, 1571
BsiSI	CCGG	4 cut(s)	93, 1080, 1560, 1960
BslFI	GGGAC	3 cut(s)	737, 1656, 1724
BslI	CCNNNNNNNGG	8 cut(s)	497, 719, 760, 1671, 2288, 2542, 2939, 3016
BsmAI	GTCTC	6 cut(s)	633, 987, 1568, 1787, 2082, 2180
BsmBI	CGTCTC	1 cut(s)	987
BsmFI	GGGAC	3 cut(s)	737, 1656, 1724
BsmI	GAATGC	3 cut(s)	139, 204, 2382
BsnI	GGCC	6 cut(s)	369, 456, 954, 1024, 1935, 1963
Bso31I	GGTCTC	1 cut(s)	1787
BsoBI	CYCGRG	2 cut(s)	492, 1571
Bsp1286I	GDGCHC	3 cut(s)	610, 1975, 2554
Bsp13I	TCCGGA	1 cut(s)	1559
Bsp1407I	TGTACA	1 cut(s)	574
Bsp1720I	GCTNAGC	1 cut(s)	555
Bsp19I	CCATGG	2 cut(s)	2985, 3010
BspACI	CCGC	9 cut(s)	14, 349, 497, 713, 770, 1131, 1858, 2943, 2999
BspANI	GGCC	6 cut(s)	369, 456, 954, 1024, 1935, 1963
BspCNI	CTCAG	9 cut(s)	280, 547, 688, 1027, 1119, 1300, 1498, 2332, 2360
BspEI	TCCGGA	1 cut(s)	1559
BspFNI	CGCG	3 cut(s)	14, 801, 3004
BspLI	GGNNCC	8 cut(s)	652, 1972, 2264, 2377, 2420, 2551, 2911, 2983
BspMAI	CTGCAG	1 cut(s)	418
BspPI	GGATC	9 cut(s)	199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
BspQI	GCTCTTC	1 cut(s)	1454
BspT107I	GGYRCC	3 cut(s)	1970, 2418, 2549
BspTNI	GGTCTC	1 cut(s)	1787
BsrBI	CCGCTC	3 cut(s)	497, 1131, 2999
BsrFI	RCCGGY	1 cut(s)	1079
BsrGI	TGTACA	1 cut(s)	574
BssAI	RCCGGY	1 cut(s)	1079
BssECI	CCNNGG	6 cut(s)	1099, 1204, 2311, 2913, 2985, 3010
BssNI	GRCGYC	2 cut(s)	2419, 2560
BssT1I	CCWWGG	4 cut(s)	1204, 2311, 2985, 3010
Bst2UI	CCWGG	3 cut(s)	1101, 1812, 1937
Bst4CI	ACNGT	7 cut(s)	172, 981, 1647, 1781, 2144, 2470, 2506
Bst6I	CTCTTC	3 cut(s)	1454, 1747, 2712
BstACI	GRCGYC	2 cut(s)	2419, 2560
BstAUI	TGTACA	1 cut(s)	574
BstC8I	GCNNGC	6 cut(s)	12, 367, 401, 2100, 2290, 2681
BstDSI	CCRYGG	3 cut(s)	2913, 2985, 3010
BstENI	CCTNNNNNAGG	1 cut(s)	2286
BstF5I	GGATG	6 cut(s)	955, 1499, 1566, 1866, 2551, 2725
BstFNI	CGCG	3 cut(s)	14, 801, 3004
BstH2I	RGCGCY	3 cut(s)	1291, 2422, 2949
BstHHI	GCGC	6 cut(s)	139, 801, 1290, 2421, 2948, 3004
BstMAI	GTCTC	6 cut(s)	633, 987, 1568, 1787, 2082, 2180
BstMWI	GCNNNNNNNGC	7 cut(s)	293, 1004, 1932, 1946, 2416, 2768, 3026
BstNI	CCWGG	3 cut(s)	1101, 1812, 1937
BstNSI	RCATGY	3 cut(s)	403, 2102, 2493
BstSCI	CCNGG	4 cut(s)	92, 1099, 1810, 1935
BstSFI	CTRYAG	3 cut(s)	414, 706, 2133
BstSLI	GKGCMC	2 cut(s)	1975, 2554
BstUI	CGCG	3 cut(s)	14, 801, 3004
BstV1I	GCAGC	7 cut(s)	400, 484, 808, 1019, 1157, 1228, 2749
BstV2I	GAAGAC	1 cut(s)	173
BstX2I	RGATCY	6 cut(s)	650, 1816, 1994, 2061, 2316, 2375
BstXI	CCANNNNNNTGG	1 cut(s)	1707
BstYI	RGATCY	6 cut(s)	650, 1816, 1994, 2061, 2316, 2375
BsuI	GTATCC	1 cut(s)	1512
BsuRI	GGCC	6 cut(s)	369, 456, 954, 1024, 1935, 1963
BtgI	CCRYGG	3 cut(s)	2913, 2985, 3010
BtgZI	GCGATG	1 cut(s)	2894
BtrI	CACGTC	1 cut(s)	2895
BtsCI	GGATG	6 cut(s)	955, 1499, 1566, 1866, 2551, 2725
BtsI	GCAGTG	1 cut(s)	1251
Cac8I	GCNNGC	6 cut(s)	12, 367, 401, 2100, 2290, 2681
CaiI	CAGNNNCTG	2 cut(s)	1319, 1425
CfoI	GCGC	6 cut(s)	139, 801, 1290, 2421, 2948, 3004
Cfr10I	RCCGGY	1 cut(s)	1079
Cfr13I	GGNCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
CseI	GACGC	1 cut(s)	2549
CspAI	ACCGGT	1 cut(s)	1079
CspCI	CAANNNNNGTGG	2 cut(s)	293, 328
DinI	GGCGCC	1 cut(s)	2420
DraIII	CACNNNGTG	1 cut(s)	326
EaeI	YGGCCR	4 cut(s)	367, 952, 1022, 1933
Eam1104I	CTCTTC	3 cut(s)	1454, 1747, 2712
EarI	CTCTTC	3 cut(s)	1454, 1747, 2712
EciI	GGCGGA	1 cut(s)	702
Eco130I	CCWWGG	4 cut(s)	1204, 2311, 2985, 3010
Eco31I	GGTCTC	1 cut(s)	1787
Eco47I	GGWCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
Eco57I	CTGAAG	5 cut(s)	1841, 1926, 2693, 2787, 2825
Eco88I	CYCGRG	2 cut(s)	492, 1571
EcoNI	CCTNNNNNAGG	1 cut(s)	2286
EcoRI	GAATTC	1 cut(s)	2048
EcoRII	CCWGG	3 cut(s)	1099, 1810, 1935
EcoT14I	CCWWGG	4 cut(s)	1204, 2311, 2985, 3010
EcoT22I	ATGCAT	1 cut(s)	405
EgeI	GGCGCC	1 cut(s)	2420
EheI	GGCGCC	1 cut(s)	2420
ErhI	CCWWGG	4 cut(s)	1204, 2311, 2985, 3010
Esp3I	CGTCTC	1 cut(s)	987
FalI	AAGNNNNNCTT	2 cut(s)	1814, 1846
FaqI	GGGAC	3 cut(s)	737, 1656, 1724
FauI	CCCGC	1 cut(s)	490
FbaI	TGATCA	1 cut(s)	175
FblI	GTMKAC	1 cut(s)	1405
FokI	GGATG	6 cut(s)	962, 1506, 1553, 1873, 2558, 2732
FspBI	CTAG	8 cut(s)	356, 452, 846, 1092, 1157, 1728, 2600, 2744
FspI	TGCGCA	1 cut(s)	138
GlaI	GCGC	6 cut(s)	138, 800, 1289, 2420, 2947, 3003
GsaI	CCCAGC	1 cut(s)	1385
GsuI	CTGGAG	2 cut(s)	1383, 2391
HaeII	RGCGCY	3 cut(s)	1291, 2422, 2949
HaeIII	GGCC	6 cut(s)	369, 456, 954, 1024, 1935, 1963
HapII	CCGG	4 cut(s)	93, 1080, 1560, 1960
HgaI	GACGC	1 cut(s)	2549
HhaI	GCGC	6 cut(s)	139, 801, 1290, 2421, 2948, 3004
Hin1I	GRCGYC	2 cut(s)	2419, 2560
Hin6I	GCGC	6 cut(s)	137, 799, 1288, 2419, 2946, 3002
HinP1I	GCGC	6 cut(s)	137, 799, 1288, 2419, 2946, 3002
HincII	GTYRAC	2 cut(s)	318, 1406
HindII	GTYRAC	2 cut(s)	318, 1406
HpaI	GTTAAC	1 cut(s)	318
HpaII	CCGG	4 cut(s)	93, 1080, 1560, 1960
HphI	GGTGA	5 cut(s)	820, 1689, 2033, 2462, 2599
Hpy166II	GTNNAC	8 cut(s)	318, 867, 1406, 1784, 2021, 2083, 2584, 2732
Hpy188I	TCNGA	7 cut(s)	267, 521, 678, 1821, 2329, 2341, 2565
Hpy8I	GTNNAC	8 cut(s)	318, 867, 1406, 1784, 2021, 2083, 2584, 2732
Hpy99I	CGWCG	2 cut(s)	2144, 2565
HpyAV	CCTTC	4 cut(s)	104, 1448, 1768, 2002
HpyCH4III	ACNGT	7 cut(s)	172, 981, 1647, 1781, 2144, 2470, 2506
HpyCH4IV	ACGT	3 cut(s)	153, 230, 2894
HpyF10VI	GCNNNNNNNGC	7 cut(s)	293, 1004, 1932, 1946, 2416, 2768, 3026
HpySE526I	ACGT	3 cut(s)	153, 230, 2894
Hsp92I	GRCGYC	2 cut(s)	2419, 2560
HspAI	GCGC	6 cut(s)	137, 799, 1288, 2419, 2946, 3002
KasI	GGCGCC	1 cut(s)	2418
Kpn2I	TCCGGA	1 cut(s)	1559
Ksp22I	TGATCA	1 cut(s)	175
KspAI	GTTAAC	1 cut(s)	318
LguI	GCTCTTC	1 cut(s)	1454
LmnI	GCTCC	3 cut(s)	551, 1733, 2776
Lsp1109I	GCAGC	7 cut(s)	400, 484, 808, 1019, 1157, 1228, 2749
LweI	GCATC	8 cut(s)	352, 985, 1201, 1228, 2397, 2484, 2645, 2802
MaeI	CTAG	8 cut(s)	356, 452, 846, 1092, 1157, 1728, 2600, 2744
MaeII	ACGT	3 cut(s)	153, 230, 2894
MaeIII	GTNAC	8 cut(s)	539, 701, 841, 1303, 1387, 1600, 1695, 2672
MbiI	CCGCTC	3 cut(s)	497, 1131, 2999
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	6 cut(s)	650, 1816, 1994, 2061, 2316, 2375
MhlI	GDGCHC	3 cut(s)	610, 1975, 2554
MlsI	TGGCCA	2 cut(s)	369, 1935
MluNI	TGGCCA	2 cut(s)	369, 1935
Mly113I	GGCGCC	1 cut(s)	2419
MmeI	TCCRAC	8 cut(s)	169, 213, 1150, 1457, 1738, 2003, 2543, 2697
Mox20I	TGGCCA	2 cut(s)	369, 1935
Mph1103I	ATGCAT	1 cut(s)	405
MroI	TCCGGA	1 cut(s)	1559
MroXI	GAANNNNTTC	1 cut(s)	1446
MscI	TGGCCA	2 cut(s)	369, 1935
MseI	TTAA	9 cut(s)	317, 804, 929, 942, 960, 1028, 1527, 2232, 2274
MslI	CAYNNNNRTG	5 cut(s)	144, 431, 1236, 2168, 2754
Msp20I	TGGCCA	2 cut(s)	369, 1935
MspI	CCGG	4 cut(s)	93, 1080, 1560, 1960
MspR9I	CCNGG	4 cut(s)	94, 1101, 1812, 1937
MunI	CAATTG	1 cut(s)	1296
Mva1269I	GAATGC	3 cut(s)	139, 204, 2382
MvaI	CCWGG	3 cut(s)	1101, 1812, 1937
MvnI	CGCG	3 cut(s)	14, 801, 3004
MwoI	GCNNNNNNNGC	7 cut(s)	293, 1004, 1932, 1946, 2416, 2768, 3026
NarI	GGCGCC	1 cut(s)	2419
NciI	CCSGG	1 cut(s)	94
NcoI	CCATGG	2 cut(s)	2985, 3010
NlaIV	GGNNCC	8 cut(s)	652, 1972, 2264, 2377, 2420, 2551, 2911, 2983
NmeAIII	GCCGAG	1 cut(s)	980
NmuCI	GTSAC	4 cut(s)	701, 841, 1303, 1695
NsbI	TGCGCA	1 cut(s)	138
NsiI	ATGCAT	1 cut(s)	405
NspI	RCATGY	3 cut(s)	403, 2102, 2493
PaeI	GCATGC	2 cut(s)	403, 2102
PaeR7I	CTCGAG	2 cut(s)	492, 1571
PciI	ACATGT	1 cut(s)	2489
PciSI	GCTCTTC	1 cut(s)	1454
PcsI	WCGNNNNNNNCGW	1 cut(s)	3001
PctI	GAATGC	3 cut(s)	139, 204, 2382
PdmI	GAANNNNTTC	1 cut(s)	1446
PflFI	GACNNNGTC	1 cut(s)	749
PflMI	CCANNNNNTGG	2 cut(s)	1671, 3016
PinAI	ACCGGT	1 cut(s)	1079
PluTI	GGCGCC	1 cut(s)	2422
PscI	ACATGT	1 cut(s)	2489
Psp6I	CCWGG	3 cut(s)	1099, 1810, 1935
PspFI	CCCAGC	1 cut(s)	1381
PspGI	CCWGG	3 cut(s)	1099, 1810, 1935
PspN4I	GGNNCC	8 cut(s)	652, 1972, 2264, 2377, 2420, 2551, 2911, 2983
PspPI	GGNCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
PspXI	VCTCGAGB	1 cut(s)	492
PsrI	GAACNNNNNNTAC	2 cut(s)	1609, 1641
PstI	CTGCAG	1 cut(s)	418
PstNI	CAGNNNCTG	2 cut(s)	1319, 1425
PsuI	RGATCY	6 cut(s)	650, 1816, 1994, 2061, 2316, 2375
PsyI	GACNNNGTC	1 cut(s)	749
RseI	CAYNNNNRTG	5 cut(s)	144, 431, 1236, 2168, 2754
SalI	GTCGAC	1 cut(s)	1404
SapI	GCTCTTC	1 cut(s)	1454
SaqAI	TTAA	9 cut(s)	317, 804, 929, 942, 960, 1028, 1527, 2232, 2274
Sau96I	GGNCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
ScaI	AGTACT	2 cut(s)	182, 1670
ScrFI	CCNGG	4 cut(s)	94, 1101, 1812, 1937
SduI	GDGCHC	3 cut(s)	610, 1975, 2554
SfaNI	GCATC	8 cut(s)	352, 985, 1201, 1228, 2397, 2484, 2645, 2802
SfcI	CTRYAG	3 cut(s)	414, 706, 2133
SfoI	GGCGCC	1 cut(s)	2420
Sfr274I	CTCGAG	2 cut(s)	492, 1571
SinI	GGWCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
SlaI	CTCGAG	2 cut(s)	492, 1571
SmiMI	CAYNNNNRTG	5 cut(s)	144, 431, 1236, 2168, 2754
SmlI	CTYRAG	3 cut(s)	492, 1571, 1853
SmoI	CTYRAG	3 cut(s)	492, 1571, 1853
SpeI	ACTAGT	2 cut(s)	355, 1091
SphI	GCATGC	2 cut(s)	403, 2102
SsiI	CCGC	9 cut(s)	14, 349, 497, 713, 770, 1131, 1858, 2943, 2999
SspDI	GGCGCC	1 cut(s)	2418
SspI	AATATT	1 cut(s)	2780
SspMI	CTAG	8 cut(s)	356, 452, 846, 1092, 1157, 1728, 2600, 2744
StyD4I	CCNGG	4 cut(s)	92, 1099, 1810, 1935
StyI	CCWWGG	4 cut(s)	1204, 2311, 2985, 3010
TaaI	ACNGT	7 cut(s)	172, 981, 1647, 1781, 2144, 2470, 2506
TaiI	ACGT	3 cut(s)	156, 233, 2897
TatI	WGTACW	4 cut(s)	180, 574, 1668, 2731
TauI	GCSGC	3 cut(s)	17, 2946, 3002
Tru1I	TTAA	9 cut(s)	317, 804, 929, 942, 960, 1028, 1527, 2232, 2274
Tru9I	TTAA	9 cut(s)	317, 804, 929, 942, 960, 1028, 1527, 2232, 2274
TseFI	GTSAC	4 cut(s)	701, 841, 1303, 1695
TseI	GCWGC	7 cut(s)	413, 472, 821, 1007, 1145, 1241, 2762
Tsp45I	GTSAC	4 cut(s)	701, 841, 1303, 1695
TspDTI	ATGAA	7 cut(s)	116, 660, 1711, 2046, 2218, 2478, 2897
TspGWI	ACGGA	5 cut(s)	504, 1038, 1451, 2406, 2729
Tth111I	GACNNNGTC	1 cut(s)	749
Van91I	CCANNNNNTGG	2 cut(s)	1671, 3016
VpaK11BI	GGWCC	5 cut(s)	1077, 1808, 2021, 2123, 2981
XagI	CCTNNNNNAGG	1 cut(s)	2286
XapI	RAATTY	3 cut(s)	1160, 1659, 2048
XbaI	TCTAGA	1 cut(s)	1727
XceI	RCATGY	3 cut(s)	403, 2102, 2493
XcmI	CCANNNNNNNNNTGG	3 cut(s)	95, 152, 2543
XhoI	CTCGAG	2 cut(s)	492, 1571
XmiI	GTMKAC	1 cut(s)	1405
XmnI	GAANNNNTTC	1 cut(s)	1446
XspI	CTAG	8 cut(s)	356, 452, 846, 1092, 1157, 1728, 2600, 2744
ZrmI	AGTACT	2 cut(s)	182, 1670
Zsp2I	ATGCAT	1 cut(s)	405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.