RH2AG126900

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0004264
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG126900.1

Sequence Viewer

Length: 2994 bp
ATGCTAAAGCTCGCGGCGTTTTTCGAGCTTGTGATTGTGGTTGTTTGGTTGGGAGTGGCGAGTGTTGATGGAGTTTTTGTGAAGTACCAGACCGGGAGTGGTGTTGTGGAAGGGAAGTTGAATGTTCATTTGGTTGCGCATTCCCATGATGACGTTGGTTGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAATAACAGCATTCAGGGTGCTTGTGTTGAGAATACGTTGGATTCTGTCGTTGAATCGTTGGTTCGTGATCCGAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTTACACAAACTGTGGAAGTTCAAGAACGAGTGCGGAAACTAGTAGATGCTGGCCAGTTAGAGTTTGTAAATGGAGGTTGGTGCATGCATGATGAGGCTACAGTCCATTACATAGACATGATTGACCAAACAACTCTAGGCCACCTTGCTATAAAGCAGCAGTTCAACAAGACACCTCGAGCGGGGTGGCAAATTGATCCGTTCGGACACTCTGCTGTGCAGGGTTACTTACTTGGAGCTGAGCTTGGATTCGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGCACAGCGTAAGGGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTCGTCACTATAGTCCGCCAGATGGTTTCCATTTTGAAGTTTTTGATGACTTTGTCCCTGTGCAGGATAATCCGCTTATTTTTGACTACAATGTTGAACTGCGTGTTAATGATTTTATTACTGCTGCAATCACCCAAGCAAATGTGACTAGGGGAAATCACATGATGTGGACAATGGGAGATGATTTCCAGTACCAATATGCTGAATCTTGGTTCAAACAAATGGATAAGTTAATTCACTATGTTAATAAGGATGGCCGAGTTAATGCCTTATATTCTACACCGTCTCTCTACACTGATGCTAAAAATGCAGCAAATCAATCATGGCCATTAAAAACTGAGGATTATTTTCCGTATGCAGATATAATAAATGCTTATTGGACCGGTTATTTTACTAGTCGCCCGGGTTTGAAAAGATATATTCGATCACTGAGCGGATATTATTTGGCAGCAAGAGAACTAGAATTTCTGGTTGGAAAGAGAGCAAATGGTTCAAGTACCTATTCCCTTGGAGATGCTTTAGGAATTGCACAGCACCACGATGCTGCCACTGGCACTGCTAAACAACATACAACAAATGACTATGTAAAGCGCCTGTCAATTGGTGTCACTGAGGCAGAAGCTGTTGTCAGTTCTGCTCTGTCGTGCCTAACTACAAATAAATCTGGAGATCAATGTAAAGACCCTGCAGTTACTTTTAGCCAGTGTCGACTACTCAATATCAGTTTCTGCCCTCCAACGGAGGAAGATATTCCAGAAGGGAAGAGCTTGGTTGTTGTGGCATATAATCCTCTTGGATGGAATCGCACTGAGATTGTTAGGATACCTGTTAATTATGATGATCTTGTTGTTCAAGATTCCTCTGGAAACATCCTCGAGACACAATATGTGAGTGTGGATAATGTTACAAGCAATCTAAGAACATTTTATACAGAAGCCTACTTGGGACAGTCATCAAAACAAATTCCCAAGTACTGGCTAATCTTTCAAGCCTCTGTGTCACCACTTGGTTGGGACACATACTTCATCTCTAGAGGAGCTACCAAAGGGAAAAGAAGAGTTGGACTTCTCTCTGTGAAGGACAGTCCACAGAATGAGACCATTGAAATTGGACCAGGAGATCTGAAGATGTCCTTTTCTTCTGCTTCTGGACAACTCAAGCGGATGTATAATTCTAAAACAGGAGTTGATGTACCAATACAACAGAGCTACCTCTGGTATGGTTCAAGCACCGATGGCCAGGCTTCAGGTGCATATATATTCCGGCCTAATGGTGCCCCTCCAACTATTGTGTCAAGATCTGTACCCTTGAAGGTCTTTCGTGGACCATTATTCGATGAGATTCACCAAGAATTCAGTTCATGGATCTATCAGGTCATTAGAGTTTACAGAGACAAAGAGCATGCCGAAGTTGAATATACTATTGGTCCAATTCCTACAGACGACGGTGTTGGTAAAGAGGTCATCACACGAATGACAGCAAACATGGAGACTAACAAGATGTTTTACACCGATTCCAATGGGAGGGATTTCATTAAAAGGGTACGAGATTATAGAGCAGACTGGAACCTTTCAGTTAATCAACCTGTTGCAGGAAACTACTATCCACTAAACCTTGGGATCTTTACTTCGGATAACAAATCTGAGTTCTCAGTCTTGGTTGATCGTGCCACTGGAGGATCCAGCATTCAAGACGGACAAGTAGAGATGATGCTACATAGAGGGGTGGGTGAAGCCCTTGATGAAACAGTGTGCATCAAGAATAATACATGTCAAGGACTAACGGTTCGAGGAAACTATTATATGAGCATAAACCAGTTCGGGGATGGTGCCCAATGGCGTCGGACAACTGGACAAGAAGTTTACTCGCCACTTCTTCTAGCTTTCACCCATGAGAACTTGGAGAATTGGACTGCATCTCATTTAACAAAAGGGTCTGTCATGGATTCAAGTTACAGCTTGCCTCCCAATGTTGCTTTGATTACTCTTCAGGAGTTGGATGGTGGAAGTGTACTTCTCCGTCTAGCACATCTATATGAGGCTGCTGAAGCTCCTCAATATTCTACATTGGCAAAAGTTGAACTGAAGAAGATGCTAACAGGAAAAACGATAAAGCAGTTGAAAGAAGTGAGCTTGTCAGCAAACCAAGAGAAGTCAGCGATGAAGAAGATGACGTGGAAAGTCGAGGGTTCCACAGGAGAAGAACCTACACCAGTAAGAGGCGGCGCTGTTGATAGTTCAACTCTTGTTGTTGAGCTTGGACCCATGGAGATACGTACTTTCTTATTGAAATTTTAG

Protein Analysis

997

Amino Acids

111.69

Weight (kDa)

5.84

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 41 - 352 2.9e-77 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 357 - 449 5e-18 Alpha mannosidase middle domain
Laman-like_dom PF21260 539 - 575 9.1e-06 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 605 - 808 3.8e-43 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 889 - 993 5.1e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 1970, 2525
AccB7I CCANNNNNTGG 1 cut(s) 1671
AccBSI CCGCTC 2 cut(s) 497, 1131
AccI GTMKAC 1 cut(s) 1405
AccII CGCG 1 cut(s) 14
AciI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2919
AclWI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AcoI YGGCCR 4 cut(s) 367, 952, 1022, 1933
AcsI RAATTY 4 cut(s) 1160, 1659, 2048, 2987
AcuI CTGAAG 5 cut(s) 1841, 1926, 2669, 2763, 2801
AcyI GRCGYC 1 cut(s) 2536
AfiI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2518, 2915
AflIII ACRYGT 1 cut(s) 2465
AgeI ACCGGT 1 cut(s) 1079
AhlI ACTAGT 2 cut(s) 355, 1091
AjiI CACGTC 1 cut(s) 2871
AjnI CCWGG 2 cut(s) 1810, 1935
AjuI GAANNNNNNNTTGG 6 cut(s) 113, 145, 1152, 1184, 2103, 2135
AloI GAACNNNNNNTCC 2 cut(s) 2466, 2498
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
AlwI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
AlwNI CAGNNNCTG 2 cut(s) 1319, 1425
Ama87I CYCGRG 3 cut(s) 492, 1099, 1571
AoxI GGCC 6 cut(s) 367, 454, 952, 1022, 1933, 1961
ApeKI GCWGC 6 cut(s) 472, 821, 1007, 1145, 1241, 2738
ApoI RAATTY 4 cut(s) 1160, 1659, 2048, 2987
ArsI GACNNNNNNTTYG 2 cut(s) 2466, 2498
AsiGI ACCGGT 1 cut(s) 1079
Asp700I GAANNNNTTC 1 cut(s) 1446
AspLEI GCGC 3 cut(s) 139, 1290, 2924
AspS9I GGNCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
AsuC2I CCSGG 3 cut(s) 94, 1100, 1101
AsuHPI GGTGA 5 cut(s) 820, 1689, 2033, 2438, 2575
AvaI CYCGRG 3 cut(s) 492, 1099, 1571
AvaII GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
BaeGI GKGCMC 2 cut(s) 1975, 2530
BalI TGGCCA 3 cut(s) 369, 1024, 1935
BamHI GGATCC 2 cut(s) 650, 2375
BanI GGYRCC 2 cut(s) 1970, 2525
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 6 cut(s) 484, 808, 1019, 1157, 1228, 2725
BccI CCATC 9 cut(s) 62, 286, 303, 713, 944, 1488, 1925, 2516, 2690
BciT130I CCWGG 2 cut(s) 1812, 1937
BciVI GTATCC 1 cut(s) 1512
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 3 cut(s) 94, 1100, 1101
BcoDI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BcuI ACTAGT 2 cut(s) 355, 1091
BfaI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2576, 2720
BfmI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BfoI RGCGCY 2 cut(s) 1291, 2925
BfuI GTATCC 1 cut(s) 1512
BglII AGATCT 2 cut(s) 1816, 1994
BisI GCNGC 8 cut(s) 15, 473, 822, 1008, 1146, 1242, 2739, 2920
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 8 cut(s) 16, 474, 823, 1009, 1147, 1243, 2740, 2921
BmcAI AGTACT 2 cut(s) 182, 1670
Bme1390I CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
Bme18I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
BmeT110I CYCGRG 3 cut(s) 492, 1099, 1571
BmgBI CACGTC 1 cut(s) 2871
BmgT120I GGNCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
BmiI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2527, 2887, 2959
BmrFI CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
BmsI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2460, 2621, 2778
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 1383, 2391
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 1838
BpuMI CCSGG 3 cut(s) 94, 1100, 1101
BsaAI YACGTR 1 cut(s) 2972
BsaHI GRCGYC 1 cut(s) 2536
BsaI GGTCTC 1 cut(s) 1787
BsaJI CCNNGG 4 cut(s) 1099, 1204, 2311, 2961
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1872, 1902
Bsc4I CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2518, 2915
Bse118I RCCGGY 1 cut(s) 1079
BseBI CCWGG 2 cut(s) 1812, 1937
BseDI CCNNGG 4 cut(s) 1099, 1204, 2311, 2961
BseGI GGATG 6 cut(s) 955, 1499, 1566, 1866, 2527, 2701
BseLI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2518, 2915
BseMII CTCAG 9 cut(s) 279, 546, 689, 1026, 1118, 1299, 1497, 2331, 2361
BseRI GAGGAG 2 cut(s) 1746, 2739
BseSI GKGCMC 2 cut(s) 1975, 2530
BseXI GCAGC 6 cut(s) 484, 808, 1019, 1157, 1228, 2725
BsgI GTGCAG 2 cut(s) 554, 779
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BshNI GGYRCC 2 cut(s) 1970, 2525
BshTI ACCGGT 1 cut(s) 1079
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 3 cut(s) 492, 1099, 1571
BsiSI CCGG 4 cut(s) 93, 1080, 1100, 1960
BslFI GGGAC 3 cut(s) 737, 1656, 1724
BslI CCNNNNNNNGG 9 cut(s) 497, 719, 760, 1435, 1671, 2220, 2288, 2518, 2915
BsmAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 3 cut(s) 737, 1656, 1724
BsmI GAATGC 3 cut(s) 139, 204, 2382
BsnI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
Bso31I GGTCTC 1 cut(s) 1787
BsoBI CYCGRG 3 cut(s) 492, 1099, 1571
Bsp1286I GDGCHC 3 cut(s) 610, 1975, 2530
Bsp1407I TGTACA 1 cut(s) 574
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 2961
BspACI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2919
BspANI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BspCNI CTCAG 9 cut(s) 280, 547, 688, 1027, 1119, 1300, 1498, 2332, 2360
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2527, 2887, 2959
BspMAI CTGCAG 1 cut(s) 1387
BspPI GGATC 9 cut(s) 199, 257, 506, 645, 658, 2069, 2324, 2370, 2383
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 2 cut(s) 1970, 2525
BspTNI GGTCTC 1 cut(s) 1787
BsrBI CCGCTC 2 cut(s) 497, 1131
BsrFI RCCGGY 1 cut(s) 1079
BsrGI TGTACA 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 4 cut(s) 1099, 1204, 2311, 2961
BssNI GRCGYC 1 cut(s) 2536
BssT1I CCWWGG 3 cut(s) 1204, 2311, 2961
Bst2UI CCWGG 2 cut(s) 1812, 1937
Bst4CI ACNGT 9 cut(s) 172, 328, 418, 981, 1647, 1781, 2144, 2446, 2482
Bst6I CTCTTC 3 cut(s) 1454, 1747, 2688
BstACI GRCGYC 1 cut(s) 2536
BstAUI TGTACA 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 2972
BstC8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2657
BstDSI CCRYGG 1 cut(s) 2961
BstENI CCTNNNNNAGG 1 cut(s) 2286
BstF5I GGATG 6 cut(s) 955, 1499, 1566, 1866, 2527, 2701
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 2 cut(s) 1291, 2925
BstHHI GCGC 3 cut(s) 139, 1290, 2924
BstMAI GTCTC 6 cut(s) 633, 987, 1568, 1787, 2082, 2180
BstMWI GCNNNNNNNGC 5 cut(s) 293, 1004, 1932, 1946, 2744
BstNI CCWGG 2 cut(s) 1812, 1937
BstNSI RCATGY 3 cut(s) 403, 2102, 2469
BstSCI CCNGG 5 cut(s) 92, 1098, 1099, 1810, 1935
BstSFI CTRYAG 4 cut(s) 414, 706, 1383, 2133
BstSLI GKGCMC 2 cut(s) 1975, 2530
BstSNI TACGTA 1 cut(s) 2972
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 6 cut(s) 484, 808, 1019, 1157, 1228, 2725
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BstXI CCANNNNNNTGG 1 cut(s) 1707
BstYI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
BsuI GTATCC 1 cut(s) 1512
BsuRI GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
BtgI CCRYGG 1 cut(s) 2961
BtgZI GCGATG 1 cut(s) 2870
BtrI CACGTC 1 cut(s) 2871
BtsCI GGATG 6 cut(s) 955, 1499, 1566, 1866, 2527, 2701
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 9 cut(s) 990, 1124, 1245, 1251, 1305, 1406, 1503, 2367, 2451
Cac8I GCNNGC 5 cut(s) 12, 367, 401, 2100, 2657
CaiI CAGNNNCTG 2 cut(s) 1319, 1425
CfoI GCGC 3 cut(s) 139, 1290, 2924
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
Cfr9I CCCGGG 1 cut(s) 1099
CseI GACGC 1 cut(s) 2525
CspAI ACCGGT 1 cut(s) 1079
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
EaeI YGGCCR 4 cut(s) 367, 952, 1022, 1933
Eam1104I CTCTTC 3 cut(s) 1454, 1747, 2688
EarI CTCTTC 3 cut(s) 1454, 1747, 2688
EciI GGCGGA 1 cut(s) 702
Eco105I TACGTA 1 cut(s) 2972
Eco130I CCWWGG 3 cut(s) 1204, 2311, 2961
Eco31I GGTCTC 1 cut(s) 1787
Eco47I GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
Eco57I CTGAAG 5 cut(s) 1841, 1926, 2669, 2763, 2801
Eco88I CYCGRG 3 cut(s) 492, 1099, 1571
EcoNI CCTNNNNNAGG 1 cut(s) 2286
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 2 cut(s) 1810, 1935
EcoT14I CCWWGG 3 cut(s) 1204, 2311, 2961
EcoT22I ATGCAT 1 cut(s) 405
ErhI CCWWGG 3 cut(s) 1204, 2311, 2961
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 2 cut(s) 1814, 1846
FaqI GGGAC 3 cut(s) 737, 1656, 1724
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 1405
Fnu4HI GCNGC 8 cut(s) 15, 473, 822, 1008, 1146, 1242, 2739, 2920
FokI GGATG 6 cut(s) 962, 1506, 1553, 1873, 2534, 2708
Fsp4HI GCNGC 8 cut(s) 15, 473, 822, 1008, 1146, 1242, 2739, 2920
FspBI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2576, 2720
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 3 cut(s) 138, 1289, 2923
GluI GCNGC 8 cut(s) 15, 473, 822, 1008, 1146, 1242, 2739, 2920
GsuI CTGGAG 2 cut(s) 1383, 2391
HaeII RGCGCY 2 cut(s) 1291, 2925
HaeIII GGCC 6 cut(s) 369, 456, 954, 1024, 1935, 1963
HapII CCGG 4 cut(s) 93, 1080, 1100, 1960
HgaI GACGC 1 cut(s) 2525
HhaI GCGC 3 cut(s) 139, 1290, 2924
Hin1I GRCGYC 1 cut(s) 2536
Hin6I GCGC 3 cut(s) 137, 1288, 2922
HinP1I GCGC 3 cut(s) 137, 1288, 2922
HincII GTYRAC 1 cut(s) 1406
HindII GTYRAC 1 cut(s) 1406
HpaII CCGG 4 cut(s) 93, 1080, 1100, 1960
HphI GGTGA 5 cut(s) 820, 1689, 2033, 2438, 2575
Hpy166II GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2560, 2708
Hpy188I TCNGA 7 cut(s) 267, 521, 678, 1821, 2329, 2341, 2541
Hpy8I GTNNAC 8 cut(s) 318, 867, 1406, 1784, 2021, 2083, 2560, 2708
Hpy99I CGWCG 2 cut(s) 2144, 2541
HpyAV CCTTC 4 cut(s) 104, 1448, 1768, 2002
HpyCH4III ACNGT 9 cut(s) 172, 328, 418, 981, 1647, 1781, 2144, 2446, 2482
HpyCH4IV ACGT 4 cut(s) 153, 230, 2870, 2971
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 1004, 1932, 1946, 2744
HpySE526I ACGT 4 cut(s) 153, 230, 2870, 2971
Hsp92I GRCGYC 1 cut(s) 2536
HspAI GCGC 3 cut(s) 137, 1288, 2922
Ksp22I TGATCA 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 3 cut(s) 551, 1733, 2752
Lsp1109I GCAGC 6 cut(s) 484, 808, 1019, 1157, 1228, 2725
LweI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2460, 2621, 2778
MaeI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2576, 2720
MaeII ACGT 4 cut(s) 153, 230, 2870, 2971
MaeIII GTNAC 8 cut(s) 539, 701, 841, 1303, 1387, 1600, 1695, 2648
MbiI CCGCTC 2 cut(s) 497, 1131
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
MhlI GDGCHC 3 cut(s) 610, 1975, 2530
MlsI TGGCCA 3 cut(s) 369, 1024, 1935
MluNI TGGCCA 3 cut(s) 369, 1024, 1935
MmeI TCCRAC 8 cut(s) 169, 213, 1150, 1457, 1738, 2003, 2519, 2673
Mox20I TGGCCA 3 cut(s) 369, 1024, 1935
Mph1103I ATGCAT 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 3 cut(s) 369, 1024, 1935
MseI TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2621
MslI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2730
Msp20I TGGCCA 3 cut(s) 369, 1024, 1935
MspI CCGG 4 cut(s) 93, 1080, 1100, 1960
MspR9I CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 3 cut(s) 139, 204, 2382
MvaI CCWGG 2 cut(s) 1812, 1937
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 5 cut(s) 293, 1004, 1932, 1946, 2744
NciI CCSGG 3 cut(s) 94, 1100, 1101
NcoI CCATGG 1 cut(s) 2961
NlaIV GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2527, 2887, 2959
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 4 cut(s) 701, 841, 1303, 1695
NsbI TGCGCA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 403, 2102, 2469
PaeI GCATGC 2 cut(s) 403, 2102
PaeR7I CTCGAG 2 cut(s) 492, 1571
PciI ACATGT 1 cut(s) 2465
PciSI GCTCTTC 1 cut(s) 1454
PctI GAATGC 3 cut(s) 139, 204, 2382
PdmI GAANNNNTTC 1 cut(s) 1446
PflFI GACNNNGTC 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 1671
PinAI ACCGGT 1 cut(s) 1079
PkrI GCNGC 8 cut(s) 16, 474, 823, 1009, 1147, 1243, 2740, 2921
Ppu21I YACGTR 1 cut(s) 2972
PscI ACATGT 1 cut(s) 2465
Psp6I CCWGG 2 cut(s) 1810, 1935
PspGI CCWGG 2 cut(s) 1810, 1935
PspN4I GGNNCC 7 cut(s) 652, 1972, 2264, 2377, 2527, 2887, 2959
PspPI GGNCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
PspXI VCTCGAGB 1 cut(s) 492
PsrI GAACNNNNNNTAC 2 cut(s) 1609, 1641
PstI CTGCAG 1 cut(s) 1387
PstNI CAGNNNCTG 2 cut(s) 1319, 1425
PsuI RGATCY 6 cut(s) 650, 1816, 1994, 2061, 2316, 2375
PsyI GACNNNGTC 1 cut(s) 749
RseI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2730
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 1 cut(s) 1454
SaqAI TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2621
SatI GCNGC 8 cut(s) 15, 473, 822, 1008, 1146, 1242, 2739, 2920
Sau96I GGNCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
ScaI AGTACT 2 cut(s) 182, 1670
ScrFI CCNGG 5 cut(s) 94, 1100, 1101, 1812, 1937
SduI GDGCHC 3 cut(s) 610, 1975, 2530
SfaNI GCATC 8 cut(s) 352, 985, 1201, 1228, 2397, 2460, 2621, 2778
SfcI CTRYAG 4 cut(s) 414, 706, 1383, 2133
Sfr274I CTCGAG 2 cut(s) 492, 1571
SinI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
SlaI CTCGAG 2 cut(s) 492, 1571
SmaI CCCGGG 1 cut(s) 1101
SmiMI CAYNNNNRTG 5 cut(s) 144, 431, 1236, 2168, 2730
SmlI CTYRAG 3 cut(s) 492, 1571, 1853
SmoI CTYRAG 3 cut(s) 492, 1571, 1853
SnaBI TACGTA 1 cut(s) 2972
SpeI ACTAGT 2 cut(s) 355, 1091
SphI GCATGC 2 cut(s) 403, 2102
SsiI CCGC 8 cut(s) 14, 349, 497, 713, 770, 1131, 1858, 2919
SspI AATATT 1 cut(s) 2756
SspMI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2576, 2720
StyD4I CCNGG 5 cut(s) 92, 1098, 1099, 1810, 1935
StyI CCWWGG 3 cut(s) 1204, 2311, 2961
TaaI ACNGT 9 cut(s) 172, 328, 418, 981, 1647, 1781, 2144, 2446, 2482
TaiI ACGT 4 cut(s) 156, 233, 2873, 2974
TaqI TCGA 9 cut(s) 24, 493, 567, 1120, 1405, 1572, 2031, 2485, 2880
TatI WGTACW 4 cut(s) 180, 574, 1668, 2707
TauI GCSGC 2 cut(s) 17, 2922
Tru1I TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2621
Tru9I TTAA 9 cut(s) 804, 929, 942, 960, 1028, 1527, 2232, 2274, 2621
TscAI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2451
TseFI GTSAC 4 cut(s) 701, 841, 1303, 1695
TseI GCWGC 6 cut(s) 472, 821, 1007, 1145, 1241, 2738
Tsp45I GTSAC 4 cut(s) 701, 841, 1303, 1695
TspDTI ATGAA 7 cut(s) 116, 660, 1711, 2046, 2218, 2454, 2873
TspGWI ACGGA 5 cut(s) 504, 1038, 1451, 2406, 2705
TspMI CCCGGG 1 cut(s) 1099
TspRI CASTG 9 cut(s) 997, 1131, 1252, 1258, 1312, 1406, 1510, 2374, 2451
Tth111I GACNNNGTC 1 cut(s) 749
Van91I CCANNNNNTGG 1 cut(s) 1671
VpaK11BI GGWCC 5 cut(s) 1077, 1808, 2021, 2123, 2957
XagI CCTNNNNNAGG 1 cut(s) 2286
XapI RAATTY 4 cut(s) 1160, 1659, 2048, 2987
XbaI TCTAGA 1 cut(s) 1727
XceI RCATGY 3 cut(s) 403, 2102, 2469
XcmI CCANNNNNNNNNTGG 3 cut(s) 95, 152, 2519
XhoI CTCGAG 2 cut(s) 492, 1571
XmaI CCCGGG 1 cut(s) 1099
XmiI GTMKAC 1 cut(s) 1405
XmnI GAANNNNTTC 1 cut(s) 1446
XspI CTAG 8 cut(s) 356, 452, 846, 1092, 1157, 1728, 2576, 2720
ZrmI AGTACT 2 cut(s) 182, 1670
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.