FvH4_1g11550

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004264
Physical Location & Seq
Forward (+)
6301301 .. 6307574
6274 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11550.t1

Sequence Viewer

Length: 3036 bp
ATGGGGAAGGGCAGTGTGGCCTCAGCTTTTCAGTGTGTTCTTATAGTAGTTGTTGTTGTTTGCTTGTGTAACAAAGCCGTTAATGGCGGTTATATGAAGTACAACACTAGCAGTGGTTTGGTGGAAGGCAAACTGAATGTTCATTTGGTTGCGCATTCCCATGATGATGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGCTGCATGTATTGAGAACGTGTTGGACTCAGTTGTGGAATCGTTGTTTCGTGATCCAAATAGGAAGTTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTAACACAAAGTGAGGAAATGCAAGAACGAGTGCGAAAGCTAGTAGAAGCTGGGCAGCTAGAGTTTGTAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGATCAGACGACACTAGGTCACCGAGCAATCAAGGAGCAGTTCAACAAGGCGCCACGAGCAGGATGGCAAATCGATCCATTTGGACACTCGGCTGTGCAGGCTTACTTGCTTGGAGCTGAGCTTGGATTTGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGAAAAGCGAAAGAAAGACAAGTCTCTTGAAGTTATCTGGCAAGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTCGCCAATGCCTTTCCTGTTCACTATGGTCCACCTCCGGGTTTCCGTTTTGAGATCAATGGTGACTTCATCCCAATTCAGGATAATCCTCTTCTCTTTGACTCCAATATTGAGGAGCGTGTTAATGATTTTGTTAATGCTGCAGCTACACAGGCTAATGTGACTAGGACAAACCACATTATGTGGAAGATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAGATGGATAAATTAATTCACTATGTTAATAAGGATGGTCGAGTTAATGCACTTTACTCTACCCCATCAATCTATACTGATGCGAAAAATGCAGCAAATCAGTCATGGCCGCTGAAGAACGATGATTATTTTCCTTATGCAGATGCGGTCCACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTTTGAAGCGATATGTAAGAATGCTGAGTGGTTACTACTTGGCAGCAAGGCAACTAGAATATCTGGTGGGGAAAAAGTCAACTGGTCCAAATACTTTTAGCCTCGGAGATGCCTTAGGAATCACGCAGCACCATGATGCTGTATCCGGCACTTCTAAGGAACACACAACAAGTGACTATGCAAAGCGCCTGTCTATTGGTGCCTCTGAGGCAGAAGCTGTTGTCAGTGCTGCTCTATCATGCCTAACCGCAGGTGAATCAGGAGAGCAATGTAAGGACCTGGCATTGACTTTTAGCCAGTGTCGATTACTCAATATCAGTTATTGCCCTCCAACTGAGGAAGAGATTCCAGAAGGAAATAGTTTGGTTATAGTGGCTTATAATCCTCTTGGATGGAATAGAACTGAGATTGTTAGAATACCTGTTAATGATGCCAATCTTGCTGTCCAAGATTCCCACGGAAATTCTATTGAGGCACAGTTCATAAGTTTGGATAACGTTACAATAAATATAAGAAATTTCTATACAAACGCCTACTTGGGAGTGTCATCAAAACAAGTCCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCGCCGCTTGGTTGGAACACATACTTCATTTCTAGACGAGCTACCAAAGAAAACAATACAAATGGATTTTTCTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAGTTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCAAACGGATGTATAATCCCAAGACAGGAGTTGATGTACCAATTCAGCAGAGCTTCCTCTGGTATAATTCAAGCTCAGAATTTCCAGGTTCTGGTGCATACCTGTTTCGCCCTAGTGAGTCTGTTCCAAAAAATGTGTCAAGATCAGTACACTTGAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACTAAACTTTACAGAGACAAACAGCATGCAGAAGTTGAGTTCACTATCGGTCCAATTCCAACAGAAGATTATAATGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGACAAGGTGTTCTACACTGATTCAAACGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGAGCAGACTGGTCCCTTTCGGTTAATGAACCAGTGGCAGGAAACTATTATCCATTAAATCTTGGCATGTTTACCTCAGATAAGAAATATGAGTTCTCGGTCTTGGTAGATCGTGCCACTGGAGGAGCTAGCATTAAAGATGGAGAAGTGGAGCTCATGCTTCATAGGCGTGTGCTCTTCGATGATGCTAAGGGAGTGGAGGAAGCCCTTGATGAAATAGTGTGCATCGATCACAGTAGTATATGTAAAGGACTAACGGTTCGAGGGAATTATTATATGAACATCAACAAACCAGGGGAAGGTGCTCGTTGGCGCAGGAAAACTGGCCAAGAAATCTACTCTCCACTTGTTTTGGCTTTCACGCATGAGAACTTGGAGAATTGGACTGCATCACATTTGTCAAAAGCATCTACAATGGATTCGAATTACAGCTTGCCGCAAAATGTAGCGCTGATTACTCTTCAGGAGTTGGATGATGGAAGTGTACTTGTCCGTGTAGCACATCTGTATGAGGCAGCTGAAGTCCCCGAGTACTCGACATTGACCAAAGTTGAACTGAAGAAGATGTTTACAGGAAAAATGATCAAGGAAGTGAAGGAGGTGAGCTTGTCAGCAAACCAAGACAAGTCAGAGATGAAGAGGATGGAATGGAAAGTTGAAGGCAATAATAACACAGAGCCTATCCCAGTTAGAGGTGGACCTGTAAATAATTCAACTCTTGTTTTTGAGCTTGGACCCATGGAGATACGAACTTTCTTGTTGAAGTTTTAG

Protein Analysis

1012

Amino Acids

114.03

Weight (kDa)

5.85

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 46 - 357 2e-81 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 362 - 454 1.5e-17 Alpha mannosidase middle domain
Laman-like_dom PF21260 531 - 580 1.4e-06 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 611 - 818 2e-46 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 903 - 1007 1.1e-07 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1497, 2160
AarI CACCTGC 1 cut(s) 1358
Acc16I TGCGCA 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 1358
AccB1I GGYRCC 2 cut(s) 502, 1316
AccB7I CCANNNNNTGG 2 cut(s) 1686, 1958
AciI CCGC 7 cut(s) 87, 1040, 1076, 1365, 1712, 1715, 2702
AclI AACGTT 1 cut(s) 1614
AclWI GGATC 6 cut(s) 214, 272, 521, 660, 673, 2084
AcoI YGGCCR 2 cut(s) 1037, 2590
AcsI RAATTY 3 cut(s) 1579, 1633, 1946
AcuI CTGAAG 5 cut(s) 1064, 1856, 2711, 2805, 2843
AcyI GRCGYC 1 cut(s) 503
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 8 cut(s) 101, 197, 591, 905, 1905, 2016, 2751, 2798
AfeI AGCGCT 1 cut(s) 2715
AflIII ACRYGT 1 cut(s) 243
AhdI GACNNNNNGTC 2 cut(s) 468, 2042
AhlI ACTAGT 2 cut(s) 1106, 2041
AjnI CCWGG 4 cut(s) 1395, 1825, 1951, 2557
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AloI GAACNNNNNNTCC 2 cut(s) 2508, 2540
Alw21I GWGCWC 3 cut(s) 2421, 2442, 2572
Alw26I GTCTC 5 cut(s) 648, 1708, 1802, 1824, 2097
AlwI GGATC 6 cut(s) 214, 272, 521, 660, 673, 2084
AlwNI CAGNNNCTG 2 cut(s) 1334, 1958
Ama87I CYCGRG 1 cut(s) 2792
Aor51HI AGCGCT 1 cut(s) 2715
AoxI GGCC 3 cut(s) 18, 1037, 2590
ApeKI GCWGC 9 cut(s) 227, 385, 836, 839, 1022, 1160, 1243, 1346, 2780
ApoI RAATTY 3 cut(s) 1579, 1633, 1946
ArsI GACNNNNNNTTYG 6 cut(s) 1292, 1324, 1988, 2020, 2508, 2540
AseI ATTAAT 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 1461
AspLEI GCGC 5 cut(s) 154, 505, 1305, 2580, 2716
AsuC2I CCSGG 1 cut(s) 735
AsuHPI GGTGA 6 cut(s) 464, 770, 905, 1382, 2048, 2878
AsuII TTCGAA 1 cut(s) 2687
AsuNHI GCTAGC 1 cut(s) 2393
AvaI CYCGRG 1 cut(s) 2792
AxyI CCTNAGG 1 cut(s) 1231
BalI TGGCCA 1 cut(s) 2592
BamHI GGATCC 1 cut(s) 665
BanI GGYRCC 2 cut(s) 502, 1316
BanII GRGCYC 1 cut(s) 2421
BauI CACGAG 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 3 cut(s) 2421, 2442, 2572
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 9 cut(s) 214, 397, 823, 851, 1034, 1172, 1255, 1333, 2792
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 4 cut(s) 1397, 1827, 1953, 2559
BciVI GTATCC 1 cut(s) 1270
BclI TGATCA 3 cut(s) 190, 454, 2847
BcnI CCSGG 1 cut(s) 735
BcoDI GTCTC 5 cut(s) 648, 1708, 1802, 1824, 2097
BcuI ACTAGT 2 cut(s) 1106, 2041
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 3 cut(s) 506, 1306, 2717
BfuAI ACCTGC 1 cut(s) 1358
BfuI GTATCC 1 cut(s) 1270
BglI GCCNNNNNGGC 1 cut(s) 1325
BlpI GCTNAGC 1 cut(s) 570
BmcAI AGTACT 2 cut(s) 197, 2798
Bme1390I CCNGG 5 cut(s) 735, 1397, 1827, 1953, 2559
BmeRI GACNNNNNGTC 2 cut(s) 468, 2042
BmeT110I CYCGRG 1 cut(s) 2792
BmiI GGNNCC 5 cut(s) 504, 667, 1318, 2279, 3001
BmrFI CCNGG 5 cut(s) 735, 1397, 1827, 1953, 2559
BmrI ACTGGG 2 cut(s) 1110, 2945
BmsI GCATC 9 cut(s) 1000, 1063, 1216, 1243, 1537, 2440, 2499, 2663, 2681
BmtI GCTAGC 1 cut(s) 2397
BmuI ACTGGG 2 cut(s) 1110, 2945
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 1848, 2406
Bpu10I CCTNAGC 2 cut(s) 22, 2454
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 2687
BpuMI CCSGG 1 cut(s) 735
Bsa29I ATCGAT 2 cut(s) 525, 2493
BsaBI GATNNNNATC 1 cut(s) 1551
BsaHI GRCGYC 1 cut(s) 503
BsaI GGTCTC 2 cut(s) 1802, 1824
BsaJI CCNNGG 4 cut(s) 1219, 1573, 2558, 3003
BsaXI ACNNNNNCTCC 2 cut(s) 1887, 1917
Bse1I ACTGG 9 cut(s) 1099, 1116, 1204, 1414, 2279, 2297, 2389, 2593, 2951
Bse21I CCTNAGG 1 cut(s) 1231
Bse3DI GCAATG 1 cut(s) 1391
Bse8I GATNNNNATC 1 cut(s) 1551
BseBI CCWGG 4 cut(s) 1397, 1827, 1953, 2559
BseCI ATCGAT 2 cut(s) 525, 2493
BseDI CCNNGG 4 cut(s) 1219, 1573, 2558, 3003
BseGI GGATG 8 cut(s) 179, 521, 765, 970, 1514, 1881, 2743, 2913
BseJI GATNNNNATC 1 cut(s) 1551
BseMI GCAATG 1 cut(s) 1391
BseNI ACTGG 9 cut(s) 1099, 1116, 1204, 1414, 2279, 2297, 2389, 2593, 2951
BseRI GAGGAG 2 cut(s) 824, 2403
BseXI GCAGC 9 cut(s) 214, 397, 823, 851, 1034, 1172, 1255, 1333, 2792
BseYI CCCAGC 1 cut(s) 380
BsgI GTGCAG 1 cut(s) 569
BshFI GGCC 3 cut(s) 20, 1039, 2592
BshNI GGYRCC 2 cut(s) 502, 1316
BshVI ATCGAT 2 cut(s) 525, 2493
BsiHKAI GWGCWC 3 cut(s) 2421, 2442, 2572
BsiHKCI CYCGRG 1 cut(s) 2792
BsiSI CCGG 2 cut(s) 734, 1263
BslFI GGGAC 3 cut(s) 1661, 2263, 2774
BsmAI GTCTC 5 cut(s) 648, 1708, 1802, 1824, 2097
BsmFI GGGAC 3 cut(s) 1661, 2263, 2774
BsmI GAATGC 3 cut(s) 154, 219, 1143
BsnI GGCC 3 cut(s) 20, 1039, 2592
Bso31I GGTCTC 2 cut(s) 1802, 1824
BsoBI CYCGRG 1 cut(s) 2792
Bsp119I TTCGAA 1 cut(s) 2687
Bsp1286I GDGCHC 3 cut(s) 2421, 2442, 2572
Bsp1407I TGTACA 1 cut(s) 589
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 1 cut(s) 3003
BspACI CCGC 7 cut(s) 87, 1040, 1076, 1365, 1712, 1715, 2702
BspANI GGCC 3 cut(s) 20, 1039, 2592
BspDI ATCGAT 2 cut(s) 525, 2493
BspLI GGNNCC 5 cut(s) 504, 667, 1318, 2279, 3001
BspMAI CTGCAG 1 cut(s) 841
BspMI ACCTGC 1 cut(s) 1358
BspOI GCTAGC 1 cut(s) 2397
BspPI GGATC 6 cut(s) 214, 272, 521, 660, 673, 2084
BspQI GCTCTTC 1 cut(s) 2447
BspT104I TTCGAA 1 cut(s) 2687
BspT107I GGYRCC 2 cut(s) 502, 1316
BspTNI GGTCTC 2 cut(s) 1802, 1824
BsrDI GCAATG 1 cut(s) 1391
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 9 cut(s) 1099, 1116, 1204, 1414, 2279, 2297, 2389, 2593, 2951
BssECI CCNNGG 4 cut(s) 1219, 1573, 2558, 3003
BssNI GRCGYC 1 cut(s) 503
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 3003
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 4 cut(s) 1397, 1827, 1953, 2559
Bst4CI ACNGT 4 cut(s) 187, 1596, 2501, 2524
Bst6I CTCTTC 6 cut(s) 792, 1452, 2161, 2447, 2730, 2897
BstACI GRCGYC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 589
BstBI TTCGAA 1 cut(s) 2687
BstC8I GCNNGC 5 cut(s) 416, 552, 2115, 2395, 2699
BstDSI CCRYGG 2 cut(s) 1573, 3003
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 8 cut(s) 179, 521, 765, 970, 1514, 1881, 2743, 2913
BstH2I RGCGCY 3 cut(s) 506, 1306, 2717
BstHHI GCGC 5 cut(s) 154, 505, 1305, 2580, 2716
BstMAI GTCTC 5 cut(s) 648, 1708, 1802, 1824, 2097
BstMWI GCNNNNNNNGC 8 cut(s) 308, 509, 551, 848, 1019, 1325, 1556, 2431
BstNI CCWGG 4 cut(s) 1397, 1827, 1953, 2559
BstNSI RCATGY 5 cut(s) 234, 418, 435, 2117, 2335
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 5 cut(s) 733, 1395, 1825, 1951, 2557
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 9 cut(s) 214, 397, 823, 851, 1034, 1172, 1255, 1333, 2792
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 2 cut(s) 665, 2076
BstYI RGATCY 2 cut(s) 665, 2076
Bsu15I ATCGAT 2 cut(s) 525, 2493
Bsu36I CCTNAGG 1 cut(s) 1231
BsuI GTATCC 1 cut(s) 1270
BsuRI GGCC 3 cut(s) 20, 1039, 2592
BsuTUI ATCGAT 2 cut(s) 525, 2493
BtgI CCRYGG 2 cut(s) 1573, 3003
BtsCI GGATG 8 cut(s) 179, 521, 765, 970, 1514, 1881, 2743, 2913
BtsI GCAGTG 2 cut(s) 19, 118
BtsIMutI CAGTG 9 cut(s) 19, 38, 118, 1348, 1421, 1713, 2220, 2304, 2382
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 5 cut(s) 416, 552, 2115, 2395, 2699
CaiI CAGNNNCTG 2 cut(s) 1334, 1958
CfoI GCGC 5 cut(s) 154, 505, 1305, 2580, 2716
ClaI ATCGAT 2 cut(s) 525, 2493
Csp6I GTAC 8 cut(s) 100, 196, 590, 904, 1904, 2015, 2750, 2797
CspCI CAANNNNNGTGG 6 cut(s) 308, 343, 1070, 1105, 2598, 2633
CviQI GTAC 8 cut(s) 100, 196, 590, 904, 1904, 2015, 2750, 2797
DinI GGCGCC 1 cut(s) 504
DraIII CACNNNGTG 1 cut(s) 341
DriI GACNNNNNGTC 2 cut(s) 468, 2042
EaeI YGGCCR 2 cut(s) 1037, 2590
Eam1104I CTCTTC 6 cut(s) 792, 1452, 2161, 2447, 2730, 2897
Eam1105I GACNNNNNGTC 2 cut(s) 468, 2042
EarI CTCTTC 6 cut(s) 792, 1452, 2161, 2447, 2730, 2897
Ecl136II GAGCTC 1 cut(s) 2419
Eco130I CCWWGG 1 cut(s) 3003
Eco24I GRGCYC 1 cut(s) 2421
Eco31I GGTCTC 2 cut(s) 1802, 1824
Eco47III AGCGCT 1 cut(s) 2715
Eco53kI GAGCTC 1 cut(s) 2419
Eco57I CTGAAG 5 cut(s) 1064, 1856, 2711, 2805, 2843
Eco81I CCTNAGG 1 cut(s) 1231
Eco88I CYCGRG 1 cut(s) 2792
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 2419
EcoO109I RGGNCCY 1 cut(s) 1393
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 4 cut(s) 1395, 1825, 1951, 2557
EcoT14I CCWWGG 1 cut(s) 3003
EcoT22I ATGCAT 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 2421
EgeI GGCGCC 1 cut(s) 504
EheI GGCGCC 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 3003
FaqI GGGAC 3 cut(s) 1661, 2263, 2774
FbaI TGATCA 3 cut(s) 190, 454, 2847
FokI GGATG 8 cut(s) 186, 528, 752, 977, 1521, 1888, 2750, 2920
FriOI GRGCYC 1 cut(s) 2421
FspI TGCGCA 1 cut(s) 153
GlaI GCGC 5 cut(s) 153, 504, 1304, 2579, 2715
GsaI CCCAGC 1 cut(s) 384
GsuI CTGGAG 2 cut(s) 1848, 2406
HaeII RGCGCY 3 cut(s) 506, 1306, 2717
HaeIII GGCC 3 cut(s) 20, 1039, 2592
HapII CCGG 2 cut(s) 734, 1263
HhaI GCGC 5 cut(s) 154, 505, 1305, 2580, 2716
Hin1I GRCGYC 1 cut(s) 503
Hin6I GCGC 5 cut(s) 152, 503, 1303, 2578, 2714
HinP1I GCGC 5 cut(s) 152, 503, 1303, 2578, 2714
HincII GTYRAC 2 cut(s) 333, 1197
HindII GTYRAC 2 cut(s) 333, 1197
HpaI GTTAAC 1 cut(s) 333
HpaII CCGG 2 cut(s) 734, 1263
HphI GGTGA 6 cut(s) 464, 770, 905, 1382, 2048, 2878
Hpy188I TCNGA 7 cut(s) 459, 693, 1223, 1324, 1945, 2344, 2896
HpyAV CCTTC 7 cut(s) 119, 1463, 2017, 2229, 2558, 2854, 2918
HpyCH4III ACNGT 4 cut(s) 187, 1596, 2501, 2524
HpyCH4IV ACGT 2 cut(s) 243, 1614
HpyF10VI GCNNNNNNNGC 8 cut(s) 308, 509, 551, 848, 1019, 1325, 1556, 2431
HpySE526I ACGT 2 cut(s) 243, 1614
Hsp92I GRCGYC 1 cut(s) 503
HspAI GCGC 5 cut(s) 152, 503, 1303, 2578, 2714
KasI GGCGCC 1 cut(s) 502
Ksp22I TGATCA 3 cut(s) 190, 454, 2847
KspAI GTTAAC 1 cut(s) 333
LguI GCTCTTC 1 cut(s) 2447
LmnI GCTCC 5 cut(s) 487, 566, 811, 2390, 2416
Lsp1109I GCAGC 9 cut(s) 214, 397, 823, 851, 1034, 1172, 1255, 1333, 2792
LweI GCATC 9 cut(s) 1000, 1063, 1216, 1243, 1537, 2440, 2499, 2663, 2681
MaeII ACGT 2 cut(s) 243, 1614
MaeIII GTNAC 8 cut(s) 68, 470, 758, 856, 1148, 1289, 1615, 2086
MflI RGATCY 2 cut(s) 665, 2076
MhlI GDGCHC 3 cut(s) 2421, 2442, 2572
MlsI TGGCCA 1 cut(s) 2592
MluNI TGGCCA 1 cut(s) 2592
Mly113I GGCGCC 1 cut(s) 503
MlyI GAGTC 4 cut(s) 245, 791, 926, 1994
MmeI TCCRAC 8 cut(s) 151, 184, 228, 1472, 1703, 1801, 2171, 2715
Mox20I TGGCCA 1 cut(s) 2592
Mph1103I ATGCAT 1 cut(s) 420
MroXI GAANNNNTTC 1 cut(s) 1461
MscI TGGCCA 1 cut(s) 2592
MslI CAYNNNNRTG 7 cut(s) 159, 165, 446, 1251, 2183, 2433, 2772
Msp20I TGGCCA 1 cut(s) 2592
MspA1I CMGCKG 2 cut(s) 1042, 2783
MspI CCGG 2 cut(s) 734, 1263
MspR9I CCNGG 5 cut(s) 735, 1397, 1827, 1953, 2559
Mva1269I GAATGC 3 cut(s) 154, 219, 1143
MvaI CCWGG 4 cut(s) 1397, 1827, 1953, 2559
MwoI GCNNNNNNNGC 8 cut(s) 308, 509, 551, 848, 1019, 1325, 1556, 2431
NarI GGCGCC 1 cut(s) 503
NciI CCSGG 1 cut(s) 735
NcoI CCATGG 1 cut(s) 3003
NheI GCTAGC 1 cut(s) 2393
NlaIV GGNNCC 5 cut(s) 504, 667, 1318, 2279, 3001
NmeAIII GCCGAG 1 cut(s) 521
NmuCI GTSAC 5 cut(s) 470, 758, 856, 1289, 2086
NsbI TGCGCA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 5 cut(s) 234, 418, 435, 2117, 2335
NspV TTCGAA 1 cut(s) 2687
PaeI GCATGC 2 cut(s) 418, 2117
PaqCI CACCTGC 1 cut(s) 1358
PciSI GCTCTTC 1 cut(s) 2447
PctI GAATGC 3 cut(s) 154, 219, 1143
PdmI GAANNNNTTC 1 cut(s) 1461
PfeI GAWTC 9 cut(s) 263, 584, 1236, 1373, 1462, 1568, 2053, 2225, 2684
PflMI CCANNNNNTGG 2 cut(s) 1686, 1958
PleI GAGTC 4 cut(s) 245, 791, 925, 1993
PluTI GGCGCC 1 cut(s) 506
PpsI GAGTC 4 cut(s) 245, 791, 925, 1993
PpuMI RGGWCCY 1 cut(s) 1393
PshBI ATTAAT 1 cut(s) 944
PsiI TTATAA 2 cut(s) 1497, 2160
Psp124BI GAGCTC 1 cut(s) 2421
Psp1406I AACGTT 1 cut(s) 1614
Psp5II RGGWCCY 1 cut(s) 1393
Psp6I CCWGG 4 cut(s) 1395, 1825, 1951, 2557
PspEI GGTNACC 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 4 cut(s) 1395, 1825, 1951, 2557
PspN4I GGNNCC 5 cut(s) 504, 667, 1318, 2279, 3001
PspPPI RGGWCCY 1 cut(s) 1393
PstI CTGCAG 1 cut(s) 841
PstNI CAGNNNCTG 2 cut(s) 1334, 1958
PsuI RGATCY 2 cut(s) 665, 2076
PvuII CAGCTG 1 cut(s) 2783
RsaI GTAC 8 cut(s) 101, 197, 591, 905, 1905, 2016, 2751, 2798
RsaNI GTAC 8 cut(s) 100, 196, 590, 904, 1904, 2015, 2750, 2797
RseI CAYNNNNRTG 7 cut(s) 159, 165, 446, 1251, 2183, 2433, 2772
SacI GAGCTC 1 cut(s) 2421
SapI GCTCTTC 1 cut(s) 2447
ScaI AGTACT 2 cut(s) 197, 2798
SchI GAGTC 4 cut(s) 245, 791, 926, 1994
ScrFI CCNGG 5 cut(s) 735, 1397, 1827, 1953, 2559
SduI GDGCHC 3 cut(s) 2421, 2442, 2572
SfaNI GCATC 9 cut(s) 1000, 1063, 1216, 1243, 1537, 2440, 2499, 2663, 2681
SfcI CTRYAG 1 cut(s) 837
SfoI GGCGCC 1 cut(s) 504
SfuI TTCGAA 1 cut(s) 2687
SmiMI CAYNNNNRTG 7 cut(s) 159, 165, 446, 1251, 2183, 2433, 2772
SpeI ACTAGT 2 cut(s) 1106, 2041
SphI GCATGC 2 cut(s) 418, 2117
SsiI CCGC 7 cut(s) 87, 1040, 1076, 1365, 1712, 1715, 2702
SspDI GGCGCC 1 cut(s) 502
SspI AATATT 1 cut(s) 805
SstI GAGCTC 1 cut(s) 2421
StyD4I CCNGG 5 cut(s) 733, 1395, 1825, 1951, 2557
StyI CCWWGG 1 cut(s) 3003
TaaI ACNGT 4 cut(s) 187, 1596, 2501, 2524
TaiI ACGT 2 cut(s) 246, 1617
TaqII GACCGA 2 cut(s) 2126, 2353
TatI WGTACW 6 cut(s) 99, 195, 589, 2014, 2749, 2796
TauI GCSGC 4 cut(s) 1042, 1714, 1717, 2704
TfiI GAWTC 9 cut(s) 263, 584, 1236, 1373, 1462, 1568, 2053, 2225, 2684
TscAI CASTG 9 cut(s) 19, 38, 118, 1348, 1421, 1713, 2227, 2304, 2389
TseFI GTSAC 5 cut(s) 470, 758, 856, 1289, 2086
TseI GCWGC 9 cut(s) 227, 385, 836, 839, 1022, 1160, 1243, 1346, 2780
Tsp45I GTSAC 5 cut(s) 470, 758, 856, 1289, 2086
TspGWI ACGGA 5 cut(s) 731, 1590, 1888, 2247, 2747
TspRI CASTG 9 cut(s) 19, 38, 118, 1348, 1421, 1713, 2227, 2304, 2389
Van91I CCANNNNNTGG 2 cut(s) 1686, 1958
VspI ATTAAT 1 cut(s) 944
XapI RAATTY 3 cut(s) 1579, 1633, 1946
XbaI TCTAGA 1 cut(s) 1742
XceI RCATGY 5 cut(s) 234, 418, 435, 2117, 2335
XcmI CCANNNNNNNNNTGG 2 cut(s) 167, 513
XmnI GAANNNNTTC 1 cut(s) 1461
ZrmI AGTACT 2 cut(s) 197, 2798
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.