Rh2AG126500

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004264
Physical Location & Seq
Forward (+)
11483174 .. 11489272
6099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG126500.1

Sequence Viewer

Length: 3027 bp
ATGGAGAAGGGCAGTGTGGCCTCAGCTTTTCATGTGCTTATTGTTCTTGTTCTTGTTTGCTTGTGTAACAAAGCTGTTAATGGCGGTTTTGTGAAGTACAACACAGGGAGTGGTGTGGTGGAAGGCAAACTAAATGTTCATTTGGTTGCGCACTCCCATGACGACGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGCTGCATGTATAGAGAATGTGTTGGACTCTGTCATGGAATCATTGGTTCGTGATCCGAATAGGAAATTTGTGTTTGCTGAGATGGCATTTTTTCAAAGATGGTGGTTAACACAAACTGTGGAAATGCAAGAACAAGTGCGCAAGCTAGTAGAAGCCGGGCAGCTAGAGTTTGTAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGACCAGACGACACTAGGTCACCGTGCAATCAAGGAGCAATTCAACAAGGCCCCCCGAACAGGATGGCAAATCGATACATTTGGACACTCGGCTGTGCAGGCTTACTTGCTTGGAGCTGAGCTTGGATTTGATTCTGTACATTTTGCAAGGGTTGATTATCAGGACAGAGAAAACCGAAAGAGAGATAAGTCGCTTGAAGTTATATGGCGTGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTGTTCACTACGGTCCACCCCCAGGTTTCCGTTTTGAAATCAATGGTGACTTCATCCCTATGCAGGATAATCCTCTACTTTTTGACTCCAATGTTGAAAAGCGTATTCATGATTTTATTAATGCTGCAGTCACACAGGCTAATGTGACAAGGACAAATCACATTATGTGGACGATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAAATGGACAAATTAATTCACTATGTTAATAAGGACGGTCGAGTTAATGCATTGTACTCTACCCCGTCAATCTATTCTGATGCAAAAAATGCAGCAAATCAGTCATGGCCACTGAAAACCGATGATTATTTTCCTTATGCAGATGTGGTCAACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTCTGAAGCGATATGTAAGAATGTTGAGTGGCTACTACTTGGCAGCAAGGCAACTAGAATTTCTGGTGGGAAAAAGGTCAACTGGTCCAAATACTTTTAGCCTTGGAGATGCTTTAGGAATTGCGCAGCACCACGATGCAGTCACAGGCACTTCTAAACAGCACACAACAAGTGACTATGCAAAGCGCCTGTCTATTGGTGCCTCTGAGGCAGAAGCTGTTGTCAGTGCTGCTCTATCATGCCTAACTACAGGTAAATCAGGAGAGCAATGTAAGGACCCGGCATTGACTTTTAGCCAGTGTCAATTACTCAATATCAGTTATTGCCCTCCAACCGAGGAAGGGATACCAGAAGGAAAGAGTTTGGTTGTAGTGGCTTATAACCCTCTTGGATGGAATAGAACTGAGAATGTTAGAATACCTGTTAATGATGCCAATCTTGCTGTCCAAGATTCCCTCGGTAATTCTCTTGAGGCACAGTTTGTAAGTTTGGATAATGTTACAATAAATATAAGAAATTTCTATACAAAAGCATACTTGGGACTGTCATCAAAACAAGTTCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCACCACTTGGTTGGAACACATACTTCATTTCTAGACGAGCTACCAGAGGAAAAAACAGAAATGGATTTTTCTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAATTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCCAACGGATGTATGATTCCAAAACAGGAGTTGATGTACCAATTCAGCAGAGCTTCCTCTGGTATAATTCAAGCTCAGAAATTCCAGGTTCTGGTGCATACATTTTTCGCCCTGAGTCCTTTCCAAAAATTGTGTCAAGATCAGTAAACTTGAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACTAGACTTTACAGAGACAAACAGCATGCGGAAGTTGAGTTCACTATCGGTCCAATTCCCACCGAAGATTATACTGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGAGAAGGTGTTCTACACTGATTCAAATGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGATCCGACTGGTCCCTTTTGGTTAACCAACCTGTAGCAGGAAATTATTATCCATTAAATCTTGGCATTTTTACCTCGGATAAGAAATATGAGTTTTCGGTCCTGGTAGATCGTGCCACTGGAGGAGCTAGCATTAAAGATGGAGAACTGGAGCTCATGCTTCATAGGCGTGTGCTCTTCGATGATCATAAAGGATTGGAGGAAGCCCTTGACGAAACAGTGTGTATCAATGGAAATAGTATATGTGAAGGACTAACGGTTCGAGGGAATTATTATGTGAACATTAACCAAGTAGGGGAAGGTGCTCGTTGGCGCCGAAAAACCGGCCAAGAAGTGTACTCTCCACTTGTTTTGACTTTCACACATGAGAAATTGGAGAAGTGGACTGCATCCCATTTGACAAAAGCATCTACAATGGATTCGAATTACACCTTGCCGCCCAATGTAGCGCTGATTACTCTTCAGGAGTTGGATGATGGAAGTGTACTTGTCCGTCTAGCACATCTATATGAGGCAGCTGAAGTCCCTGAGTACTCGACATTAACCAAAGTTGAACTGAAGAAGATGTTTACAGGAAAAATGATAAAGGAAGTGAAGGAGATGAGCTTGTCAGCAAACCAAGACAAGTCGGAGATGAAGAGGATGGAATGGAAAGTTGAAGGTAATGACACAGAGCCTATCCCAGTTAGAGGGGGACCTGTGAATAATTCAACTCTTCTTGTTGAGCTTGGACCCATGGAGATACGAACTTTCTTGCTGAAGTTTTAG

Protein Analysis

1008

Amino Acids

113.8

Weight (kDa)

6.14

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 45 - 356 4.4e-79 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 361 - 453 2.1e-18 Alpha mannosidase middle domain
Laman-like_dom PF21260 530 - 579 4.3e-07 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 609 - 816 4.1e-46 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 901 - 1004 6.8e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1494
Acc16I TGCGCA 3 cut(s) 150, 362, 1239
AccB1I GGYRCC 2 cut(s) 1313, 2571
AccB7I CCANNNNNTGG 3 cut(s) 1683, 1715, 1955
AciI CCGC 3 cut(s) 84, 2111, 2696
AclWI GGATC 6 cut(s) 211, 269, 657, 670, 2078, 2256
AcoI YGGCCR 2 cut(s) 1034, 2584
AcsI RAATTY 4 cut(s) 287, 1172, 1630, 1944
AcuI CTGAAG 5 cut(s) 1139, 1853, 2705, 2799, 2837
AcyI GRCGYC 1 cut(s) 2572
AfaI GTAC 9 cut(s) 98, 194, 588, 902, 983, 1902, 2597, 2745, 2792
AfeI AGCGCT 1 cut(s) 2709
AhdI GACNNNNNGTC 2 cut(s) 465, 2036
AhlI ACTAGT 2 cut(s) 1103, 2035
AjnI CCWGG 4 cut(s) 730, 1822, 1948, 2361
AloI GAACNNNNNNTCC 4 cut(s) 2105, 2137, 2502, 2534
Alw21I GWGCWC 3 cut(s) 2415, 2436, 2566
Alw26I GTCTC 4 cut(s) 1705, 1799, 1821, 2091
AlwI GGATC 6 cut(s) 211, 269, 657, 670, 2078, 2256
AlwNI CAGNNNCTG 2 cut(s) 1331, 1955
Aor51HI AGCGCT 1 cut(s) 2709
AoxI GGCC 4 cut(s) 18, 498, 1034, 2584
ApeKI GCWGC 8 cut(s) 224, 382, 833, 1019, 1157, 1240, 1343, 2774
ApoI RAATTY 4 cut(s) 287, 1172, 1630, 1944
ArsI GACNNNNNNTTYG 6 cut(s) 1289, 1321, 1982, 2014, 2502, 2534
AseI ATTAAT 2 cut(s) 828, 941
Asp700I GAANNNNTTC 1 cut(s) 2207
AspLEI GCGC 6 cut(s) 151, 363, 1240, 1302, 2574, 2710
AsuC2I CCSGG 2 cut(s) 379, 1394
AsuHPI GGTGA 4 cut(s) 461, 767, 902, 2042
AsuII TTCGAA 1 cut(s) 2681
AsuNHI GCTAGC 1 cut(s) 2387
BalI TGGCCA 1 cut(s) 1036
BamHI GGATCC 1 cut(s) 662
BanI GGYRCC 2 cut(s) 1313, 2571
BanII GRGCYC 1 cut(s) 2415
BarI GAAGNNNNNNTAC 2 cut(s) 2195, 2227
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 3 cut(s) 2415, 2436, 2566
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 8 cut(s) 211, 394, 820, 1031, 1169, 1252, 1330, 2786
BciT130I CCWGG 4 cut(s) 732, 1824, 1950, 2363
BciVI GTATCC 1 cut(s) 1452
BclI TGATCA 2 cut(s) 187, 2443
BcnI CCSGG 2 cut(s) 379, 1394
BcoDI GTCTC 4 cut(s) 1705, 1799, 1821, 2091
BcuI ACTAGT 2 cut(s) 1103, 2035
BfmI CTRYAG 3 cut(s) 834, 1362, 2292
BfoI RGCGCY 3 cut(s) 1303, 2575, 2711
BfuI GTATCC 1 cut(s) 1452
BglI GCCNNNNNGGC 1 cut(s) 1322
BisI GCNGC 9 cut(s) 225, 383, 834, 1020, 1158, 1241, 1344, 2696, 2775
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 9 cut(s) 226, 384, 835, 1021, 1159, 1242, 1345, 2697, 2776
BmcAI AGTACT 2 cut(s) 194, 2792
Bme1390I CCNGG 6 cut(s) 379, 732, 1394, 1824, 1950, 2363
BmeRI GACNNNNNGTC 2 cut(s) 465, 2036
BmiI GGNNCC 8 cut(s) 501, 664, 1315, 1392, 2273, 2573, 2955, 2992
BmrFI CCNGG 6 cut(s) 379, 732, 1394, 1824, 1950, 2363
BmrI ACTGGG 2 cut(s) 1107, 2936
BmsI GCATC 6 cut(s) 997, 1213, 1240, 1534, 2657, 2675
BmtI GCTAGC 1 cut(s) 2391
BmuI ACTGGG 2 cut(s) 1107, 2936
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 3 cut(s) 1845, 2400, 2429
Bpu10I CCTNAGC 1 cut(s) 22
Bpu1102I GCTNAGC 1 cut(s) 567
Bpu14I TTCGAA 1 cut(s) 2681
BpuEI CTTGAG 1 cut(s) 1604
BpuMI CCSGG 2 cut(s) 379, 1394
Bsa29I ATCGAT 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 1548
BsaHI GRCGYC 1 cut(s) 2572
BsaI GGTCTC 2 cut(s) 1799, 1821
BsaJI CCNNGG 6 cut(s) 730, 1216, 1449, 1570, 2334, 2994
BsaXI ACNNNNNCTCC 2 cut(s) 1884, 1914
Bse118I RCCGGY 1 cut(s) 2582
Bse1I ACTGG 9 cut(s) 1096, 1113, 1201, 1411, 2161, 2273, 2383, 2412, 2942
Bse3DI GCAATG 1 cut(s) 1388
Bse8I GATNNNNATC 1 cut(s) 1548
BseBI CCWGG 4 cut(s) 732, 1824, 1950, 2363
BseCI ATCGAT 1 cut(s) 522
BseDI CCNNGG 6 cut(s) 730, 1216, 1449, 1570, 2334, 2994
BseGI GGATG 8 cut(s) 176, 518, 762, 1511, 1878, 2648, 2737, 2907
BseJI GATNNNNATC 1 cut(s) 1548
BseMI GCAATG 1 cut(s) 1388
BseNI ACTGG 9 cut(s) 1096, 1113, 1201, 1411, 2161, 2273, 2383, 2412, 2942
BseRI GAGGAG 1 cut(s) 2397
BseXI GCAGC 8 cut(s) 211, 394, 820, 1031, 1169, 1252, 1330, 2786
BsgI GTGCAG 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 967
BshFI GGCC 4 cut(s) 20, 500, 1036, 2586
BshNI GGYRCC 2 cut(s) 1313, 2571
BshVI ATCGAT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 967
BsiHKAI GWGCWC 3 cut(s) 2415, 2436, 2566
BsiSI CCGG 3 cut(s) 378, 1394, 2583
BslFI GGGAC 4 cut(s) 1668, 2257, 2768, 2967
BsmAI GTCTC 4 cut(s) 1705, 1799, 1821, 2091
BsmFI GGGAC 4 cut(s) 1668, 2257, 2768, 2967
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 4 cut(s) 20, 500, 1036, 2586
Bso31I GGTCTC 2 cut(s) 1799, 1821
Bsp119I TTCGAA 1 cut(s) 2681
Bsp1286I GDGCHC 3 cut(s) 2415, 2436, 2566
Bsp1407I TGTACA 1 cut(s) 586
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 2994
BspACI CCGC 3 cut(s) 84, 2111, 2696
BspANI GGCC 4 cut(s) 20, 500, 1036, 2586
BspDI ATCGAT 1 cut(s) 522
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 8 cut(s) 501, 664, 1315, 1392, 2273, 2573, 2955, 2992
BspMAI CTGCAG 1 cut(s) 838
BspOI GCTAGC 1 cut(s) 2391
BspPI GGATC 6 cut(s) 211, 269, 657, 670, 2078, 2256
BspQI GCTCTTC 1 cut(s) 2441
BspT104I TTCGAA 1 cut(s) 2681
BspT107I GGYRCC 2 cut(s) 1313, 2571
BspTNI GGTCTC 2 cut(s) 1799, 1821
BsrDI GCAATG 1 cut(s) 1388
BsrFI RCCGGY 1 cut(s) 2582
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 9 cut(s) 1096, 1113, 1201, 1411, 2161, 2273, 2383, 2412, 2942
BssAI RCCGGY 1 cut(s) 2582
BssECI CCNNGG 6 cut(s) 730, 1216, 1449, 1570, 2334, 2994
BssNI GRCGYC 1 cut(s) 2572
BssT1I CCWWGG 2 cut(s) 1216, 2994
Bst2UI CCWGG 4 cut(s) 732, 1824, 1950, 2363
Bst4CI ACNGT 9 cut(s) 184, 340, 473, 722, 965, 1593, 1659, 2479, 2518
Bst6I CTCTTC 5 cut(s) 2155, 2441, 2724, 2891, 2979
BstACI GRCGYC 1 cut(s) 2572
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstAUI TGTACA 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 2681
BstC8I GCNNGC 5 cut(s) 365, 413, 549, 2109, 2389
BstDSI CCRYGG 1 cut(s) 2994
BstEII GGTNACC 1 cut(s) 467
BstENI CCTNNNNNAGG 1 cut(s) 2295
BstF5I GGATG 8 cut(s) 176, 518, 762, 1511, 1878, 2648, 2737, 2907
BstH2I RGCGCY 3 cut(s) 1303, 2575, 2711
BstHHI GCGC 6 cut(s) 151, 363, 1240, 1302, 2574, 2710
BstMAI GTCTC 4 cut(s) 1705, 1799, 1821, 2091
BstMCI CGRYCG 1 cut(s) 967
BstMWI GCNNNNNNNGC 6 cut(s) 305, 548, 1016, 1322, 1553, 2425
BstNI CCWGG 4 cut(s) 732, 1824, 1950, 2363
BstNSI RCATGY 4 cut(s) 231, 415, 432, 2111
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 6 cut(s) 377, 730, 1392, 1822, 1948, 2361
BstSFI CTRYAG 3 cut(s) 834, 1362, 2292
BstV1I GCAGC 8 cut(s) 211, 394, 820, 1031, 1169, 1252, 1330, 2786
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 3 cut(s) 662, 2070, 2261
BstXI CCANNNNNNTGG 1 cut(s) 1719
BstYI RGATCY 3 cut(s) 662, 2070, 2261
Bsu15I ATCGAT 1 cut(s) 522
BsuI GTATCC 1 cut(s) 1452
BsuRI GGCC 4 cut(s) 20, 500, 1036, 2586
BsuTUI ATCGAT 1 cut(s) 522
BtgI CCRYGG 1 cut(s) 2994
BtsCI GGATG 8 cut(s) 176, 518, 762, 1511, 1878, 2648, 2737, 2907
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 8 cut(s) 19, 1037, 1345, 1418, 1710, 2214, 2376, 2484
Cac8I GCNNGC 5 cut(s) 365, 413, 549, 2109, 2389
CaiI CAGNNNCTG 2 cut(s) 1331, 1955
CciI TCATGA 1 cut(s) 817
CfoI GCGC 6 cut(s) 151, 363, 1240, 1302, 2574, 2710
Cfr10I RCCGGY 1 cut(s) 2582
ClaI ATCGAT 1 cut(s) 522
Csp6I GTAC 9 cut(s) 97, 193, 587, 901, 982, 1901, 2596, 2744, 2791
CspCI CAANNNNNGTGG 4 cut(s) 305, 340, 2592, 2627
CviQI GTAC 9 cut(s) 97, 193, 587, 901, 982, 1901, 2596, 2744, 2791
DinI GGCGCC 1 cut(s) 2573
DriI GACNNNNNGTC 2 cut(s) 465, 2036
EaeI YGGCCR 2 cut(s) 1034, 2584
Eam1104I CTCTTC 5 cut(s) 2155, 2441, 2724, 2891, 2979
Eam1105I GACNNNNNGTC 2 cut(s) 465, 2036
EarI CTCTTC 5 cut(s) 2155, 2441, 2724, 2891, 2979
Ecl136II GAGCTC 1 cut(s) 2413
Eco130I CCWWGG 2 cut(s) 1216, 2994
Eco24I GRGCYC 1 cut(s) 2415
Eco31I GGTCTC 2 cut(s) 1799, 1821
Eco47III AGCGCT 1 cut(s) 2709
Eco53kI GAGCTC 1 cut(s) 2413
Eco57I CTGAAG 5 cut(s) 1139, 1853, 2705, 2799, 2837
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 2413
EcoNI CCTNNNNNAGG 1 cut(s) 2295
EcoO109I RGGNCCY 3 cut(s) 499, 1390, 2954
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 4 cut(s) 730, 1822, 1948, 2361
EcoT14I CCWWGG 2 cut(s) 1216, 2994
EcoT22I ATGCAT 2 cut(s) 417, 979
EcoT38I GRGCYC 1 cut(s) 2415
EgeI GGCGCC 1 cut(s) 2573
EheI GGCGCC 1 cut(s) 2573
ErhI CCWWGG 2 cut(s) 1216, 2994
FaqI GGGAC 4 cut(s) 1668, 2257, 2768, 2967
FbaI TGATCA 2 cut(s) 187, 2443
Fnu4HI GCNGC 9 cut(s) 225, 383, 834, 1020, 1158, 1241, 1344, 2696, 2775
FokI GGATG 8 cut(s) 183, 525, 749, 1518, 1885, 2635, 2744, 2914
FriOI GRGCYC 1 cut(s) 2415
Fsp4HI GCNGC 9 cut(s) 225, 383, 834, 1020, 1158, 1241, 1344, 2696, 2775
FspI TGCGCA 3 cut(s) 150, 362, 1239
GlaI GCGC 6 cut(s) 150, 362, 1239, 1301, 2573, 2709
GluI GCNGC 9 cut(s) 225, 383, 834, 1020, 1158, 1241, 1344, 2696, 2775
GsuI CTGGAG 3 cut(s) 1845, 2400, 2429
HaeII RGCGCY 3 cut(s) 1303, 2575, 2711
HaeIII GGCC 4 cut(s) 20, 500, 1036, 2586
HapII CCGG 3 cut(s) 378, 1394, 2583
HhaI GCGC 6 cut(s) 151, 363, 1240, 1302, 2574, 2710
Hin1I GRCGYC 1 cut(s) 2572
Hin6I GCGC 6 cut(s) 149, 361, 1238, 1300, 2572, 2708
HinP1I GCGC 6 cut(s) 149, 361, 1238, 1300, 2572, 2708
HincII GTYRAC 4 cut(s) 330, 1078, 1194, 2284
HindII GTYRAC 4 cut(s) 330, 1078, 1194, 2284
HpaI GTTAAC 2 cut(s) 330, 2284
HpaII CCGG 3 cut(s) 378, 1394, 2583
HphI GGTGA 4 cut(s) 461, 767, 902, 2042
Hpy188I TCNGA 9 cut(s) 279, 690, 1006, 1119, 1321, 1942, 2266, 2338, 2890
Hpy99I CGWCG 1 cut(s) 167
HpyCH4III ACNGT 9 cut(s) 184, 340, 473, 722, 965, 1593, 1659, 2479, 2518
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 548, 1016, 1322, 1553, 2425
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 2572
HspAI GCGC 6 cut(s) 149, 361, 1238, 1300, 2572, 2708
KasI GGCGCC 1 cut(s) 2571
Ksp22I TGATCA 2 cut(s) 187, 2443
KspAI GTTAAC 2 cut(s) 330, 2284
LguI GCTCTTC 1 cut(s) 2441
LmnI GCTCC 4 cut(s) 484, 563, 2384, 2410
Lsp1109I GCAGC 8 cut(s) 211, 394, 820, 1031, 1169, 1252, 1330, 2786
LweI GCATC 6 cut(s) 997, 1213, 1240, 1534, 2657, 2675
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 9 cut(s) 65, 467, 755, 838, 853, 1255, 1286, 1612, 2080
MflI RGATCY 3 cut(s) 662, 2070, 2261
MhlI GDGCHC 3 cut(s) 2415, 2436, 2566
MlsI TGGCCA 1 cut(s) 1036
MluNI TGGCCA 1 cut(s) 1036
Mly113I GGCGCC 1 cut(s) 2572
MlyI GAGTC 4 cut(s) 242, 788, 923, 1988
MmeI TCCRAC 9 cut(s) 148, 181, 225, 1469, 1700, 1891, 2289, 2709, 2868
Mox20I TGGCCA 1 cut(s) 1036
Mph1103I ATGCAT 2 cut(s) 417, 979
MroXI GAANNNNTTC 1 cut(s) 2207
MscI TGGCCA 1 cut(s) 1036
MslI CAYNNNNRTG 7 cut(s) 156, 443, 767, 1248, 2177, 2427, 2766
Msp20I TGGCCA 1 cut(s) 1036
MspA1I CMGCKG 1 cut(s) 2777
MspI CCGG 3 cut(s) 378, 1394, 2583
MspR9I CCNGG 6 cut(s) 379, 732, 1394, 1824, 1950, 2363
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 4 cut(s) 732, 1824, 1950, 2363
MwoI GCNNNNNNNGC 6 cut(s) 305, 548, 1016, 1322, 1553, 2425
NarI GGCGCC 1 cut(s) 2572
NciI CCSGG 2 cut(s) 379, 1394
NcoI CCATGG 1 cut(s) 2994
NheI GCTAGC 1 cut(s) 2387
NlaIV GGNNCC 8 cut(s) 501, 664, 1315, 1392, 2273, 2573, 2955, 2992
NmeAIII GCCGAG 1 cut(s) 518
NmuCI GTSAC 7 cut(s) 467, 755, 838, 853, 1255, 1286, 2080
NsbI TGCGCA 3 cut(s) 150, 362, 1239
NsiI ATGCAT 2 cut(s) 417, 979
NspI RCATGY 4 cut(s) 231, 415, 432, 2111
NspV TTCGAA 1 cut(s) 2681
PaeI GCATGC 2 cut(s) 415, 2111
PagI TCATGA 1 cut(s) 817
PciSI GCTCTTC 1 cut(s) 2441
PcsI WCGNNNNNNNCGW 1 cut(s) 2572
PctI GAATGC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 2207
PfeI GAWTC 7 cut(s) 260, 581, 1565, 1880, 2047, 2219, 2678
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 3 cut(s) 1683, 1715, 1955
PkrI GCNGC 9 cut(s) 226, 384, 835, 1021, 1159, 1242, 1345, 2697, 2776
PleI GAGTC 4 cut(s) 242, 788, 922, 1987
PluTI GGCGCC 1 cut(s) 2575
PpsI GAGTC 4 cut(s) 242, 788, 922, 1987
PpuMI RGGWCCY 2 cut(s) 1390, 2954
PshBI ATTAAT 2 cut(s) 828, 941
PsiI TTATAA 1 cut(s) 1494
Psp124BI GAGCTC 1 cut(s) 2415
Psp5II RGGWCCY 2 cut(s) 1390, 2954
Psp6I CCWGG 4 cut(s) 730, 1822, 1948, 2361
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 4 cut(s) 730, 1822, 1948, 2361
PspN4I GGNNCC 8 cut(s) 501, 664, 1315, 1392, 2273, 2573, 2955, 2992
PspPPI RGGWCCY 2 cut(s) 1390, 2954
PstI CTGCAG 1 cut(s) 838
PstNI CAGNNNCTG 2 cut(s) 1331, 1955
PsuI RGATCY 3 cut(s) 662, 2070, 2261
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 2777
RsaI GTAC 9 cut(s) 98, 194, 588, 902, 983, 1902, 2597, 2745, 2792
RsaNI GTAC 9 cut(s) 97, 193, 587, 901, 982, 1901, 2596, 2744, 2791
RseI CAYNNNNRTG 7 cut(s) 156, 443, 767, 1248, 2177, 2427, 2766
SacI GAGCTC 1 cut(s) 2415
SapI GCTCTTC 1 cut(s) 2441
SatI GCNGC 9 cut(s) 225, 383, 834, 1020, 1158, 1241, 1344, 2696, 2775
ScaI AGTACT 2 cut(s) 194, 2792
SchI GAGTC 4 cut(s) 242, 788, 923, 1988
ScrFI CCNGG 6 cut(s) 379, 732, 1394, 1824, 1950, 2363
SduI GDGCHC 3 cut(s) 2415, 2436, 2566
SfaNI GCATC 6 cut(s) 997, 1213, 1240, 1534, 2657, 2675
SfcI CTRYAG 3 cut(s) 834, 1362, 2292
SfoI GGCGCC 1 cut(s) 2573
SfuI TTCGAA 1 cut(s) 2681
SmiMI CAYNNNNRTG 7 cut(s) 156, 443, 767, 1248, 2177, 2427, 2766
SmlI CTYRAG 1 cut(s) 1583
SmoI CTYRAG 1 cut(s) 1583
SpeI ACTAGT 2 cut(s) 1103, 2035
SphI GCATGC 2 cut(s) 415, 2111
SsiI CCGC 3 cut(s) 84, 2111, 2696
SspDI GGCGCC 1 cut(s) 2571
SstI GAGCTC 1 cut(s) 2415
StyD4I CCNGG 6 cut(s) 377, 730, 1392, 1822, 1948, 2361
StyI CCWWGG 2 cut(s) 1216, 2994
TaaI ACNGT 9 cut(s) 184, 340, 473, 722, 965, 1593, 1659, 2479, 2518
TaiI ACGT 1 cut(s) 168
TaqI TCGA 9 cut(s) 522, 897, 967, 2040, 2251, 2439, 2521, 2681, 2795
TaqII GACCGA 2 cut(s) 2120, 2347
TatI WGTACW 7 cut(s) 96, 192, 586, 981, 2595, 2743, 2790
TauI GCSGC 1 cut(s) 2698
TfiI GAWTC 7 cut(s) 260, 581, 1565, 1880, 2047, 2219, 2678
TscAI CASTG 8 cut(s) 19, 1044, 1345, 1418, 1710, 2221, 2383, 2484
TseFI GTSAC 7 cut(s) 467, 755, 838, 853, 1255, 1286, 2080
TseI GCWGC 8 cut(s) 224, 382, 833, 1019, 1157, 1240, 1343, 2774
Tsp45I GTSAC 7 cut(s) 467, 755, 838, 853, 1255, 1286, 2080
TspGWI ACGGA 3 cut(s) 728, 1885, 2741
TspRI CASTG 8 cut(s) 19, 1044, 1345, 1418, 1710, 2221, 2383, 2484
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 3 cut(s) 1683, 1715, 1955
VspI ATTAAT 2 cut(s) 828, 941
XagI CCTNNNNNAGG 1 cut(s) 2295
XapI RAATTY 4 cut(s) 287, 1172, 1630, 1944
XbaI TCTAGA 1 cut(s) 1739
XceI RCATGY 4 cut(s) 231, 415, 432, 2111
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 1716
XmnI GAANNNNTTC 1 cut(s) 2207
ZrmI AGTACT 2 cut(s) 194, 2792
Zsp2I ATGCAT 2 cut(s) 417, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.