RchiOBHm_Chr2g0099191

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004264
Physical Location & Seq
Forward (+)
11450866 .. 11456970
6105 bp
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UTR
Exon/CDS
Intron
PRQ47391

Sequence Viewer

Length: 3111 bp
ATGGAGAAGGGCAGTGTGGCCACAGCTTTTCATGTGCTTATTGTTGTTATTGTTTGCTTGTGTAACAAAGCTGTTAGTGGCGGTTTTGTGAAGTACAACACAGGGAGTGGTGTGGTGGAAGGCAAACTAAATGTTCATTTGGTTGCGCACTCCCATGACGACGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGCTGCATGTATAGAGAATGTGTTGGACTCTGTCATGGAATCATTGGTTCGTGATCCGAATAGGAAATTTGTGTTTGCTGAGATGGCATTTTTTCAAAGATGGTGGTTAACACAAACTGTGGAAATGCAAGAACAAGTGCGCAAGCTAGTAGAAGCCGGGCAGCTAGAGTTTGTAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGACCAGACGACACTAGGTCACCGTGCAATCAAGGAGCAATTCAACAAGGCCCCCCGAGCAGGATGGCAAATCGATACATTTGGACACTCGGCTGTGCAGGCTTACTTGCTTGGAGCTGAGCTTGGATTTGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGAAAAGCGAAAGAGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTGTTCACTACGGTCCACCCCCAGGTTTCCGTTTTGAAATTAATGGTGACTTCATCCCTATTCAGGATAATCCTTTACTTTTTGACTCCAATGTTGAAAAGCGTGTTCATGATTTTATTAATGCTGCATTCACACAGGCTAATGTGACAAGGACAAATCACATTATGTGGACAATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAAATGGACAAATTAATTCACTATGTTAATAAGGACGGTCGAGTTAATGCATTGTACTCTACCCCGTCAATCTATTCTGATGCAAAAAATGCAGCAAATCAGTCATGGCCACTGAAAACCGATGATTATTTTCCTTATGCAGATGTGGTCAACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTCTGAAGCGATATGTAAGAATGTTGAGCGGCTACTACTTGGCCGCAAGGCAACTAGAATTTCTGGTGGGAAAAAGGTCAACTGGTCCAAATACTTTTAGCCTTGGAGATGCTTTAGGAATTGCGCAGCACCACGATGCAGTCACAGGCACTTCTAAACAGCACACAACAAATGACTATGCAAAGCGCCTGTCTATTGGTGCCTCTGAGGCAGAAGCTGTTGTCAGTGCTGCTCTATCATGCCTAACTACAGGTAAATCAGGAGAGCAATGTAAGGACCCGGCATTGACTTTTAGCCAGTGTCAATTACTCAATATCAGTTATTGCCCTCCAACCGAGGAAGGGATACCTGAAGGAGAGAGTTTGGTTGTAGTGGCTTATAACCCTCTTGGATGGAATAGAACTGAGAATGTTAGAATACCTGTTAATGATGCCAGTCTTGCTGTCCAAGATTCCCTCGGTAATTCTCTTGAGGCACAGTTTGTAAGTTTGGATAATGTTACAATAAATATAAGAAATTTCTATACAAAAGCATACTTGGGACTGTCATCAAAACAAGTTCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCACCACTTGGTTGGAACACATACTTCATTTCTAGACGACCTACCAGAGGAAAAAACAGAAATGGATTTTTCTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAATTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCCAACGGATGTATGATTCCAAAACAGGAGTTGATGTACCAATTCAGCAGAGCTTCCTCTGGTATAATTCAAGCTCAGAAATTCCAGGTTCTGGTGCATACATTTTTCGCCCTGAGTCCTTTCCAAAAATTGCGTCAAGATCAGTAAACTTAAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACTAGACTTTACAGAGACAAACAGCATGCGGAAGTTGAGTTCACTATCGGTCCAATTCCCAACGAAGATTATACTGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGAGAAGGTGTTCTACACTGATTCGAATGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGATCCGACTGGTCCCTTTTGGTTAACCAACCTGTAGCAGGAAATTATTATCCATTAAATCTTGGCATTTTTACCTCGGAGAAGAAATATGAGTTTTCGGTCCTGGTAGATCGTGCCACTGGAGGAGCTAGCATTAGAGATGGAGAACTGGAGCTCATGCTTCATAGGCGTGTGCTCTTCGATGATCATAAAGGATTGGAGGAAGCCCTTGACGAAACAGTGTGTATCAATGGAAATAGTATATGTGAAGGACTAACGGTTCGAGGGAATTATTATGTGAACATTAACCAAGTAGGGGAAGGTGCTCGTTGGCGCCGAAAAACTGGCCAAGAAGTGTACTCTCCACTTGTTTTGGCTTTCACACATGAGAAATTGGAGAAGTGGACTGCATGCCATTTGACAAAAGCATCTACAATGGATTCGAATTACACCTTGCCGCCCAATGTAGCGCTGATTACTCTTCAGTTGGATGATGGAAGTGTACTAGTCCGTCTAGCACATCTATATGAGGCAGCTGAAGTTCCCGAGTACTCGACATTAACCAAAGTTGAACTGAAGAAGATGTTTTCAGGAAAAATGGTATTTATATCATGTGTTTACACTGGTATTAACTGGTCCAGTAGGGAAATTACTCAATTATGCTATTGCTGGTGTGTGAAATTTTTTCAGATAAAGGAAGTGAAGGAGATGAGCTTGTCAGCAAACCAAGACAAGTCGGAGATGAAGAGGATGGAATGGAAAGTTGAAGGTAATGACACAGAGCCTATCCCAGTTAGAGGGGGGCCTGTAAATAATTCAACTCTTCTTGTTGAGCTTGGACCCATGGAGATACGAACTTTCTTGCTGAAGTTTTAG

Protein Analysis

1036

Amino Acids

117.19

Weight (kDa)

6.2

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 44 - 355 1.7e-79 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 360 - 452 9.1e-19 Alpha mannosidase middle domain
Laman-like_dom PF21260 529 - 578 4.5e-07 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 608 - 815 4e-45 Glycosyl hydrolases family 38 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1491
Acc16I TGCGCA 3 cut(s) 147, 359, 1236
AccB1I GGYRCC 2 cut(s) 1310, 2568
AccB7I CCANNNNNTGG 3 cut(s) 1680, 1712, 1952
AccBSI CCGCTC 1 cut(s) 1140
AciI CCGC 5 cut(s) 81, 1140, 1155, 2108, 2693
AclWI GGATC 6 cut(s) 208, 266, 654, 667, 2075, 2253
AcoI YGGCCR 4 cut(s) 18, 1031, 1152, 2581
AcsI RAATTY 5 cut(s) 284, 1169, 1627, 1941, 2915
AcuI CTGAAG 6 cut(s) 1136, 1482, 1850, 2702, 2793, 2831
AcyI GRCGYC 1 cut(s) 2569
AfaI GTAC 9 cut(s) 95, 191, 585, 899, 980, 1899, 2594, 2739, 2786
AfeI AGCGCT 1 cut(s) 2706
AhdI GACNNNNNGTC 2 cut(s) 462, 2033
AhlI ACTAGT 3 cut(s) 1100, 2032, 2740
AjnI CCWGG 4 cut(s) 727, 1819, 1945, 2358
AloI GAACNNNNNNTCC 4 cut(s) 2102, 2134, 2499, 2531
Alw21I GWGCWC 3 cut(s) 2412, 2433, 2563
Alw26I GTCTC 5 cut(s) 642, 1702, 1796, 1818, 2088
AlwI GGATC 6 cut(s) 208, 266, 654, 667, 2075, 2253
AlwNI CAGNNNCTG 2 cut(s) 1328, 1952
Ama87I CYCGRG 2 cut(s) 501, 2780
Aor51HI AGCGCT 1 cut(s) 2706
AoxI GGCC 6 cut(s) 18, 495, 1031, 1152, 2581, 3038
ApeKI GCWGC 7 cut(s) 221, 379, 830, 1016, 1237, 1340, 2768
ApoI RAATTY 5 cut(s) 284, 1169, 1627, 1941, 2915
ArsI GACNNNNNNTTYG 6 cut(s) 1286, 1318, 1979, 2011, 2499, 2531
AseI ATTAAT 3 cut(s) 747, 825, 938
Asp700I GAANNNNTTC 1 cut(s) 2204
AspLEI GCGC 6 cut(s) 148, 360, 1237, 1299, 2571, 2707
AsuC2I CCSGG 2 cut(s) 376, 1391
AsuHPI GGTGA 4 cut(s) 458, 764, 899, 2039
AsuII TTCGAA 2 cut(s) 2219, 2678
AsuNHI GCTAGC 1 cut(s) 2384
AvaI CYCGRG 2 cut(s) 501, 2780
BalI TGGCCA 3 cut(s) 20, 1033, 2583
BamHI GGATCC 1 cut(s) 659
BanI GGYRCC 2 cut(s) 1310, 2568
BanII GRGCYC 1 cut(s) 2412
BarI GAAGNNNNNNTAC 2 cut(s) 2192, 2224
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 3 cut(s) 2412, 2433, 2563
BbvI GCAGC 7 cut(s) 208, 391, 817, 1028, 1249, 1327, 2780
BccI CCATC 9 cut(s) 162, 295, 312, 504, 1497, 1777, 2390, 2723, 2980
BciT130I CCWGG 4 cut(s) 729, 1821, 1947, 2360
BciVI GTATCC 1 cut(s) 1449
BclI TGATCA 2 cut(s) 184, 2440
BcnI CCSGG 2 cut(s) 376, 1391
BcoDI GTCTC 5 cut(s) 642, 1702, 1796, 1818, 2088
BcuI ACTAGT 3 cut(s) 1100, 2032, 2740
BfmI CTRYAG 2 cut(s) 1359, 2289
BfoI RGCGCY 3 cut(s) 1300, 2572, 2708
BfuI GTATCC 1 cut(s) 1449
BglI GCCNNNNNGGC 1 cut(s) 1319
BlpI GCTNAGC 1 cut(s) 564
BmcAI AGTACT 2 cut(s) 191, 2786
Bme1390I CCNGG 6 cut(s) 376, 729, 1391, 1821, 1947, 2360
BmeRI GACNNNNNGTC 2 cut(s) 462, 2033
BmeT110I CYCGRG 2 cut(s) 501, 2780
BmiI GGNNCC 8 cut(s) 498, 661, 1312, 1389, 2270, 2570, 3039, 3076
BmrFI CCNGG 6 cut(s) 376, 729, 1391, 1821, 1947, 2360
BmrI ACTGGG 2 cut(s) 1104, 3020
BmsI GCATC 5 cut(s) 994, 1210, 1237, 1531, 2672
BmtI GCTAGC 1 cut(s) 2388
BmuI ACTGGG 2 cut(s) 1104, 3020
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 3 cut(s) 1842, 2397, 2426
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 2 cut(s) 2219, 2678
BpuEI CTTGAG 1 cut(s) 1601
BpuMI CCSGG 2 cut(s) 376, 1391
Bsa29I ATCGAT 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 2569
BsaI GGTCTC 2 cut(s) 1796, 1818
BsaJI CCNNGG 6 cut(s) 727, 1213, 1446, 1567, 2331, 3078
BsaXI ACNNNNNCTCC 2 cut(s) 1881, 1911
Bse3DI GCAATG 1 cut(s) 1385
BseBI CCWGG 4 cut(s) 729, 1821, 1947, 2360
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 6 cut(s) 727, 1213, 1446, 1567, 2331, 3078
BseGI GGATG 7 cut(s) 173, 515, 759, 1508, 1875, 2731, 2991
BseMI GCAATG 1 cut(s) 1385
BseMII CTCAG 9 cut(s) 288, 555, 698, 900, 1308, 1506, 1713, 1950, 1965
BseRI GAGGAG 1 cut(s) 2394
BseXI GCAGC 7 cut(s) 208, 391, 817, 1028, 1249, 1327, 2780
BsgI GTGCAG 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 964
BshFI GGCC 6 cut(s) 20, 497, 1033, 1154, 2583, 3040
BshNI GGYRCC 2 cut(s) 1310, 2568
BshVI ATCGAT 1 cut(s) 519
BsiEI CGRYCG 1 cut(s) 964
BsiHKAI GWGCWC 3 cut(s) 2412, 2433, 2563
BsiHKCI CYCGRG 2 cut(s) 501, 2780
BsiSI CCGG 2 cut(s) 375, 1391
BslFI GGGAC 2 cut(s) 1665, 2254
BsmAI GTCTC 5 cut(s) 642, 1702, 1796, 1818, 2088
BsmFI GGGAC 2 cut(s) 1665, 2254
BsmI GAATGC 2 cut(s) 213, 833
BsnI GGCC 6 cut(s) 20, 497, 1033, 1154, 2583, 3040
Bso31I GGTCTC 2 cut(s) 1796, 1818
BsoBI CYCGRG 2 cut(s) 501, 2780
Bsp119I TTCGAA 2 cut(s) 2219, 2678
Bsp1286I GDGCHC 3 cut(s) 2412, 2433, 2563
Bsp1407I TGTACA 1 cut(s) 583
Bsp1720I GCTNAGC 1 cut(s) 564
Bsp19I CCATGG 1 cut(s) 3078
BspACI CCGC 5 cut(s) 81, 1140, 1155, 2108, 2693
BspANI GGCC 6 cut(s) 20, 497, 1033, 1154, 2583, 3040
BspCNI CTCAG 9 cut(s) 289, 556, 697, 901, 1309, 1507, 1712, 1949, 1966
BspDI ATCGAT 1 cut(s) 519
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 8 cut(s) 498, 661, 1312, 1389, 2270, 2570, 3039, 3076
BspOI GCTAGC 1 cut(s) 2388
BspPI GGATC 6 cut(s) 208, 266, 654, 667, 2075, 2253
BspQI GCTCTTC 1 cut(s) 2438
BspT104I TTCGAA 2 cut(s) 2219, 2678
BspT107I GGYRCC 2 cut(s) 1310, 2568
BspTNI GGTCTC 2 cut(s) 1796, 1818
BsrBI CCGCTC 1 cut(s) 1140
BsrDI GCAATG 1 cut(s) 1385
BsrGI TGTACA 1 cut(s) 583
BssECI CCNNGG 6 cut(s) 727, 1213, 1446, 1567, 2331, 3078
BssNI GRCGYC 1 cut(s) 2569
BssT1I CCWWGG 2 cut(s) 1213, 3078
Bst2UI CCWGG 4 cut(s) 729, 1821, 1947, 2360
Bst4CI ACNGT 9 cut(s) 181, 337, 470, 719, 962, 1590, 1656, 2476, 2515
Bst6I CTCTTC 5 cut(s) 2152, 2438, 2721, 2975, 3063
BstACI GRCGYC 1 cut(s) 2569
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 583
BstBI TTCGAA 2 cut(s) 2219, 2678
BstC8I GCNNGC 6 cut(s) 362, 410, 546, 2106, 2386, 2647
BstDEI CTNAG 9 cut(s) 297, 564, 684, 909, 1317, 1515, 1699, 1936, 1974
BstDSI CCRYGG 1 cut(s) 3078
BstEII GGTNACC 1 cut(s) 464
BstENI CCTNNNNNAGG 2 cut(s) 1749, 2292
BstF5I GGATG 7 cut(s) 173, 515, 759, 1508, 1875, 2731, 2991
BstH2I RGCGCY 3 cut(s) 1300, 2572, 2708
BstHHI GCGC 6 cut(s) 148, 360, 1237, 1299, 2571, 2707
BstMAI GTCTC 5 cut(s) 642, 1702, 1796, 1818, 2088
BstMCI CGRYCG 1 cut(s) 964
BstMWI GCNNNNNNNGC 7 cut(s) 302, 503, 545, 1013, 1319, 1550, 2422
BstNI CCWGG 4 cut(s) 729, 1821, 1947, 2360
BstNSI RCATGY 5 cut(s) 228, 412, 429, 2108, 2649
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 6 cut(s) 374, 727, 1389, 1819, 1945, 2358
BstSFI CTRYAG 2 cut(s) 1359, 2289
BstV1I GCAGC 7 cut(s) 208, 391, 817, 1028, 1249, 1327, 2780
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 3 cut(s) 659, 2067, 2258
BstXI CCANNNNNNTGG 1 cut(s) 1716
BstYI RGATCY 3 cut(s) 659, 2067, 2258
Bsu15I ATCGAT 1 cut(s) 519
BsuI GTATCC 1 cut(s) 1449
BsuRI GGCC 6 cut(s) 20, 497, 1033, 1154, 2583, 3040
BsuTUI ATCGAT 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 3078
BtsCI GGATG 7 cut(s) 173, 515, 759, 1508, 1875, 2731, 2991
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 9 cut(s) 19, 1034, 1342, 1415, 1707, 2211, 2373, 2481, 2856
Cac8I GCNNGC 6 cut(s) 362, 410, 546, 2106, 2386, 2647
CaiI CAGNNNCTG 2 cut(s) 1328, 1952
CciI TCATGA 1 cut(s) 814
CfoI GCGC 6 cut(s) 148, 360, 1237, 1299, 2571, 2707
ClaI ATCGAT 1 cut(s) 519
CseI GACGC 1 cut(s) 1983
Csp6I GTAC 9 cut(s) 94, 190, 584, 898, 979, 1898, 2593, 2738, 2785
CspCI CAANNNNNGTGG 4 cut(s) 302, 337, 2589, 2624
CviQI GTAC 9 cut(s) 94, 190, 584, 898, 979, 1898, 2593, 2738, 2785
DdeI CTNAG 9 cut(s) 297, 564, 684, 909, 1317, 1515, 1699, 1936, 1974
DinI GGCGCC 1 cut(s) 2570
DriI GACNNNNNGTC 2 cut(s) 462, 2033
EaeI YGGCCR 4 cut(s) 18, 1031, 1152, 2581
Eam1104I CTCTTC 5 cut(s) 2152, 2438, 2721, 2975, 3063
Eam1105I GACNNNNNGTC 2 cut(s) 462, 2033
EarI CTCTTC 5 cut(s) 2152, 2438, 2721, 2975, 3063
Ecl136II GAGCTC 1 cut(s) 2410
Eco130I CCWWGG 2 cut(s) 1213, 3078
Eco24I GRGCYC 1 cut(s) 2412
Eco31I GGTCTC 2 cut(s) 1796, 1818
Eco47III AGCGCT 1 cut(s) 2706
Eco53kI GAGCTC 1 cut(s) 2410
Eco57I CTGAAG 6 cut(s) 1136, 1482, 1850, 2702, 2793, 2831
Eco88I CYCGRG 2 cut(s) 501, 2780
Eco91I GGTNACC 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 2410
EcoNI CCTNNNNNAGG 2 cut(s) 1749, 2292
EcoO109I RGGNCCY 3 cut(s) 496, 1387, 3038
EcoO65I GGTNACC 1 cut(s) 464
EcoRII CCWGG 4 cut(s) 727, 1819, 1945, 2358
EcoT14I CCWWGG 2 cut(s) 1213, 3078
EcoT22I ATGCAT 2 cut(s) 414, 976
EcoT38I GRGCYC 1 cut(s) 2412
EgeI GGCGCC 1 cut(s) 2570
EheI GGCGCC 1 cut(s) 2570
ErhI CCWWGG 2 cut(s) 1213, 3078
FaqI GGGAC 2 cut(s) 1665, 2254
FbaI TGATCA 2 cut(s) 184, 2440
FokI GGATG 7 cut(s) 180, 522, 746, 1515, 1882, 2738, 2998
FriOI GRGCYC 1 cut(s) 2412
FspI TGCGCA 3 cut(s) 147, 359, 1236
GlaI GCGC 6 cut(s) 147, 359, 1236, 1298, 2570, 2706
GsuI CTGGAG 3 cut(s) 1842, 2397, 2426
HaeII RGCGCY 3 cut(s) 1300, 2572, 2708
HaeIII GGCC 6 cut(s) 20, 497, 1033, 1154, 2583, 3040
HapII CCGG 2 cut(s) 375, 1391
HgaI GACGC 1 cut(s) 1983
HhaI GCGC 6 cut(s) 148, 360, 1237, 1299, 2571, 2707
Hin1I GRCGYC 1 cut(s) 2569
Hin6I GCGC 6 cut(s) 146, 358, 1235, 1297, 2569, 2705
HinP1I GCGC 6 cut(s) 146, 358, 1235, 1297, 2569, 2705
HincII GTYRAC 4 cut(s) 327, 1075, 1191, 2281
HindII GTYRAC 4 cut(s) 327, 1075, 1191, 2281
HpaI GTTAAC 2 cut(s) 327, 2281
HpaII CCGG 2 cut(s) 375, 1391
HphI GGTGA 4 cut(s) 458, 764, 899, 2039
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 9 cut(s) 113, 1445, 1457, 2194, 2220, 2498, 2549, 2932, 2996
HpyCH4III ACNGT 9 cut(s) 181, 337, 470, 719, 962, 1590, 1656, 2476, 2515
HpyCH4IV ACGT 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 7 cut(s) 302, 503, 545, 1013, 1319, 1550, 2422
HpyF3I CTNAG 9 cut(s) 297, 564, 684, 909, 1317, 1515, 1699, 1936, 1974
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 2569
HspAI GCGC 6 cut(s) 146, 358, 1235, 1297, 2569, 2705
KasI GGCGCC 1 cut(s) 2568
Ksp22I TGATCA 2 cut(s) 184, 2440
KspAI GTTAAC 2 cut(s) 327, 2281
LguI GCTCTTC 1 cut(s) 2438
LmnI GCTCC 4 cut(s) 481, 560, 2381, 2407
Lsp1109I GCAGC 7 cut(s) 208, 391, 817, 1028, 1249, 1327, 2780
LweI GCATC 5 cut(s) 994, 1210, 1237, 1531, 2672
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 7 cut(s) 62, 464, 752, 850, 1252, 1609, 2077
MbiI CCGCTC 1 cut(s) 1140
MflI RGATCY 3 cut(s) 659, 2067, 2258
MhlI GDGCHC 3 cut(s) 2412, 2433, 2563
MlsI TGGCCA 3 cut(s) 20, 1033, 2583
MluNI TGGCCA 3 cut(s) 20, 1033, 2583
Mly113I GGCGCC 1 cut(s) 2569
MlyI GAGTC 4 cut(s) 239, 785, 920, 1985
MmeI TCCRAC 9 cut(s) 145, 178, 222, 1466, 1697, 1888, 2286, 2703, 2952
Mox20I TGGCCA 3 cut(s) 20, 1033, 2583
Mph1103I ATGCAT 2 cut(s) 414, 976
MroXI GAANNNNTTC 1 cut(s) 2204
MscI TGGCCA 3 cut(s) 20, 1033, 2583
MslI CAYNNNNRTG 6 cut(s) 153, 440, 1245, 2174, 2424, 2760
Msp20I TGGCCA 3 cut(s) 20, 1033, 2583
MspA1I CMGCKG 1 cut(s) 2771
MspI CCGG 2 cut(s) 375, 1391
MspR9I CCNGG 6 cut(s) 376, 729, 1391, 1821, 1947, 2360
Mva1269I GAATGC 2 cut(s) 213, 833
MvaI CCWGG 4 cut(s) 729, 1821, 1947, 2360
MwoI GCNNNNNNNGC 7 cut(s) 302, 503, 545, 1013, 1319, 1550, 2422
NarI GGCGCC 1 cut(s) 2569
NciI CCSGG 2 cut(s) 376, 1391
NcoI CCATGG 1 cut(s) 3078
NheI GCTAGC 1 cut(s) 2384
NlaIV GGNNCC 8 cut(s) 498, 661, 1312, 1389, 2270, 2570, 3039, 3076
NmeAIII GCCGAG 1 cut(s) 515
NmuCI GTSAC 5 cut(s) 464, 752, 850, 1252, 2077
NsbI TGCGCA 3 cut(s) 147, 359, 1236
NsiI ATGCAT 2 cut(s) 414, 976
NspI RCATGY 5 cut(s) 228, 412, 429, 2108, 2649
NspV TTCGAA 2 cut(s) 2219, 2678
PaeI GCATGC 3 cut(s) 412, 2108, 2649
PagI TCATGA 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 2438
PcsI WCGNNNNNNNCGW 1 cut(s) 2569
PctI GAATGC 2 cut(s) 213, 833
PdmI GAANNNNTTC 1 cut(s) 2204
PfeI GAWTC 7 cut(s) 257, 578, 1562, 1877, 2044, 2216, 2675
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 3 cut(s) 1680, 1712, 1952
PleI GAGTC 4 cut(s) 239, 785, 919, 1984
PluTI GGCGCC 1 cut(s) 2572
PpsI GAGTC 4 cut(s) 239, 785, 919, 1984
PpuMI RGGWCCY 1 cut(s) 1387
PshBI ATTAAT 3 cut(s) 747, 825, 938
PsiI TTATAA 1 cut(s) 1491
Psp124BI GAGCTC 1 cut(s) 2412
Psp5II RGGWCCY 1 cut(s) 1387
Psp6I CCWGG 4 cut(s) 727, 1819, 1945, 2358
PspEI GGTNACC 1 cut(s) 464
PspGI CCWGG 4 cut(s) 727, 1819, 1945, 2358
PspN4I GGNNCC 8 cut(s) 498, 661, 1312, 1389, 2270, 2570, 3039, 3076
PspPPI RGGWCCY 1 cut(s) 1387
PstNI CAGNNNCTG 2 cut(s) 1328, 1952
PsuI RGATCY 3 cut(s) 659, 2067, 2258
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 2771
RsaI GTAC 9 cut(s) 95, 191, 585, 899, 980, 1899, 2594, 2739, 2786
RsaNI GTAC 9 cut(s) 94, 190, 584, 898, 979, 1898, 2593, 2738, 2785
RseI CAYNNNNRTG 6 cut(s) 153, 440, 1245, 2174, 2424, 2760
SacI GAGCTC 1 cut(s) 2412
SapI GCTCTTC 1 cut(s) 2438
ScaI AGTACT 2 cut(s) 191, 2786
SchI GAGTC 4 cut(s) 239, 785, 920, 1985
ScrFI CCNGG 6 cut(s) 376, 729, 1391, 1821, 1947, 2360
SduI GDGCHC 3 cut(s) 2412, 2433, 2563
SfaNI GCATC 5 cut(s) 994, 1210, 1237, 1531, 2672
SfcI CTRYAG 2 cut(s) 1359, 2289
SfoI GGCGCC 1 cut(s) 2570
SfuI TTCGAA 2 cut(s) 2219, 2678
SmiMI CAYNNNNRTG 6 cut(s) 153, 440, 1245, 2174, 2424, 2760
SmlI CTYRAG 1 cut(s) 1580
SmoI CTYRAG 1 cut(s) 1580
SpeI ACTAGT 3 cut(s) 1100, 2032, 2740
SphI GCATGC 3 cut(s) 412, 2108, 2649
SsiI CCGC 5 cut(s) 81, 1140, 1155, 2108, 2693
SspDI GGCGCC 1 cut(s) 2568
SstI GAGCTC 1 cut(s) 2412
StyD4I CCNGG 6 cut(s) 374, 727, 1389, 1819, 1945, 2358
StyI CCWWGG 2 cut(s) 1213, 3078
TaaI ACNGT 9 cut(s) 181, 337, 470, 719, 962, 1590, 1656, 2476, 2515
TaiI ACGT 1 cut(s) 165
TaqII GACCGA 2 cut(s) 2117, 2344
TatI WGTACW 7 cut(s) 93, 189, 583, 978, 2592, 2737, 2784
TauI GCSGC 3 cut(s) 1143, 1157, 2695
TfiI GAWTC 7 cut(s) 257, 578, 1562, 1877, 2044, 2216, 2675
TscAI CASTG 9 cut(s) 19, 1041, 1342, 1415, 1707, 2218, 2380, 2481, 2863
TseFI GTSAC 5 cut(s) 464, 752, 850, 1252, 2077
TseI GCWGC 7 cut(s) 221, 379, 830, 1016, 1237, 1340, 2768
Tsp45I GTSAC 5 cut(s) 464, 752, 850, 1252, 2077
TspGWI ACGGA 3 cut(s) 725, 1882, 2735
TspRI CASTG 9 cut(s) 19, 1041, 1342, 1415, 1707, 2218, 2380, 2481, 2863
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 3 cut(s) 1680, 1712, 1952
VspI ATTAAT 3 cut(s) 747, 825, 938
XagI CCTNNNNNAGG 2 cut(s) 1749, 2292
XapI RAATTY 5 cut(s) 284, 1169, 1627, 1941, 2915
XbaI TCTAGA 1 cut(s) 1736
XceI RCATGY 5 cut(s) 228, 412, 429, 2108, 2649
XcmI CCANNNNNNNNNTGG 2 cut(s) 161, 1713
XmnI GAANNNNTTC 1 cut(s) 2204
ZrmI AGTACT 2 cut(s) 191, 2786
Zsp2I ATGCAT 2 cut(s) 414, 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.