Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004264

Physical Location & Seq

Genomic Coordinates Forward (+)

10671285 .. 10677375

Gene Structure 6091 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016854

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGGAGAAGGGCAGTGTGGCCTCAGCTTTTCATGTGCTTATTGTTGTTGTTGTTGTTATTGTTTGCTTGTGTAACAAAGCTGTTAATGGCGGTTTTGTGAAGTACAACACAGGGAGTGGTGTGGTGGAAGGCAAACTAAATGTTCATTTGGTTGCACACTCCCATGACGACGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGCTGCATGTATAGAGAATGTGTTGGACTCTGTCATGGAATCGTTGGTTCGTGATCCGAATAGGAAATTTGTGTTTGCTGAGATGGCATTTTTCCAAAGATGGTGGTTAACACAAACTGTGGAAATGCAAGAACAAGTGCGAAAGCTAGTAGAAGCCGGGCGGCTAGAGTTTGTAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGACCAGACGACACTAGGTCACCATGCAATCAAGGAGCAATTCAACAAGGCCCCCCGAGCAGGATGGCAAATTGATACATTTGGACACTCCGCTGTGCAGGCTTACTTGCTCGGAGCTGAGCTAGGATTTGATTCTGTACATTTTGCAAGGATTGACTATCAGGACAGAGAAAAGCGAAAGAGAGAAAAGTCTCTTGAAGTTATATGGCGTGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTGTTCACTACGGTCCACCCCCAGGTTTCCGTTTTGAAATCAATGGTGACTTCATCCCTATTCAGGATAATCCTCTACTTTTTGACTCCAATGTTGAAAAGCGTGTTCATGATTTTATTAATGCTGCAGTCGCACAGGCTAATGTGACAAGGACAAATCACATTATGTGGACAATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAAATGGACAAATTAATTCACTATGTTAATAAGGACGCTCGAGTTAATGCATTGTACTCTACCCCATCAATCTATTCTGATGCAAAAAATGCAGCAAATCAGTCATGGCCACTGAAAACCGACGATTATTTTCCTTATGCAGATGCGGTCAACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTCTGAAGCGATATGTAAGAATGTTGAGTGGCTACTACTTGGCGGCAAGGCAGCTAGAATTTCTGGGGGGGAAAAGGTCAACTGGTCCAAATACTTTTAGCCTTGGAGATGCTTTAGGAATCGCGCAGCACCACGATGCAGTCACAGGCACTTCTAAACAGCACACAACAAGTGACTATGCAAAGCGCCTGTCTATTGGTGCCTCTGAGGCAGAAGCTGTTGTCAGTGCTGCTCTATCATGCCTAACTACAGGTAAATCAGGAGAGCAATGTAAGGACCCGGCATTGACTTTTAGCCAGTGTCAATTACTCAATATCAGTTATTGCCCTCCAACCGAGGGAGGGATACCAGAAGGAAAGAGTTTGGTTGTAGTGGCTTATAACCCTCTTGGATGGAATAGAACTGAGAATGTTAGAATACCTGTTAATGATGCCAATCTTGCTGTCCAAGATTCCCTCGGTAATTCTCTTGAGGCACAGTTTGTAAGTTTGGATAATGTTACAATAAATATAAGAAATTTCTATACAAAAGCATACTTGGGACTGTCATCAAAGCAAGTTCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCACCACTTGGTTGGAACACATACTTCATTTCTACACTAGCTACCAGAGGAAAAAACAGAAATGGATTTTTCTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAATTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCCAACGGATGTATGATTCCAAAACAGGAGTTGATGTACCGATTCAGCAGAGCTTCCTTTGGTATAATTCAAGCTCAGAAGTTCCAGGTTCTGGTGCATACATTTTTCGCCCTAGTGAGTCCTTTCCAAAAATTGTGTCAAGATCAGTAAACTTGAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACTAGACTTTACAGAGACAAACAGCACGCGGAAGTTGAGTTCACTATCGGTCCAATTCCCACCGAAGATTATACTGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGAGAAGGTGTTCTACACTGATTCAAATGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGATCCGACTGGTCCCTTTTGGTTAACCAACCTATAGCGGGAAATTATTATCCATTAAATCTTGGCATCTTTACCTTGGATAAGAAATATGAGTTTTCGGTCCTGGTAGATCGTGCCACTGGAGGAGCTAGCATTAAAGATGGAGAACTGGAGCTCATGCTTCATAGGCGTGTGGTCTTCGATGATCATAAAGGATTGGAGGAAGCCCTTGATGAAACAGTGTGTATCAATGGAAATAGTATATGTGAAGGACTAACGGTTCGAGGGAATTATTATGTGAACATTAACCAAGTAGGGGAAGGTGCTCGTTGGCGCCGAAAAACTGGCCAAGAAGTGTACTTTCCACTTGTTTTGGCTTTCACACATGAGAAATTGGAGAAGTGGACTGCAACTCATTTGACAAAAGCATCTACAATGGATTCGAATTACACCTTGCCGCCCAATGTAGCACTGATTACTCTTCAGGAGTTGGATGATGGAAGTGTACTTGTCCGTCTAGCACATCTATATGAGGCAGCTGAAGTCCCTGAGTACTCGACATTAACCAAAGTTGAACTGAAGAAGATGTTTACAGGAAAAATGATAAAGGAAGTGAAGGAGATGAGCTTGTCAGCAAACCAAGACAAGTCGGAGATGAAGAGGATGGAATGGAAAGTTGAAGGTAATGACACAGAGCCTATCCCAGTTAGAGGGGGACCTGTAAATAATTCAACTCTTCTTGTTGAGCTTGGACCCATGGAGATACGAACTTTCTTGCTGAAGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009987 GO:0015923 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901135 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1012

Amino Acids

113.86

Weight (kDa)

6.16

Isoelectric Point (pI)

36.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	47 - 358	2.3e-79	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	363 - 455	2e-18	Alpha mannosidase middle domain
Laman-like_dom	PF21260	532 - 581	4.3e-07	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	611 - 819	1.2e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	904 - 1007	6.9e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66150 AT5G66150 AT5G66150
fragaria_vesca	FvH4_1g11550 FvH4_1g11600 FvH4_1g11600 FvH4_1g11600
malus_domestica	MD02G1128700.v1.1 MD02G1129000.v1.1
prunus_persica	Prupe.7G170500_v2.0.a1 Prupe.7G170500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0099191 RchiOBHm_Chr2g0099201
rosa_laevigata	RLG00000016854 RLG00000016860
rosa_multiflora	Rmu_co8341649.1_g000001 Rmu_sc0002025.1_g000008 Rmu_sc0010993.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143340 Rroxscaffold_2G00143370
rosa_rugosa	Rorug02G0077600 Rorug02G0078100 Rorug02G0078100
rosa_samantha	Rh2AG126500 Rh2AG126900 Rh2BG129700 Rh2BG129900 Rh2CG130700 Rh2CG131100 Rh2DG131100 Rh2DG131500
rosa_wichuraiana	Rw2G009780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1500
AccB1I	GGYRCC	2 cut(s)	1319, 2580
AccB7I	CCANNNNNTGG	3 cut(s)	1689, 1721, 1961
AccII	CGCG	2 cut(s)	1244, 2120
AciI	CCGC	8 cut(s)	90, 388, 546, 1079, 1163, 2120, 2306, 2705
AclWI	GGATC	6 cut(s)	217, 275, 663, 676, 2087, 2265
AcoI	YGGCCR	2 cut(s)	1040, 2593
AcsI	RAATTY	3 cut(s)	293, 1178, 1636
AcuI	CTGAAG	5 cut(s)	1145, 1859, 2714, 2808, 2846
AcyI	GRCGYC	1 cut(s)	2581
AfaI	GTAC	9 cut(s)	104, 200, 594, 908, 989, 1908, 2606, 2754, 2801
AhdI	GACNNNNNGTC	2 cut(s)	471, 2045
AhlI	ACTAGT	2 cut(s)	1109, 2044
AjnI	CCWGG	4 cut(s)	736, 1828, 1954, 2370
AloI	GAACNNNNNNTCC	4 cut(s)	2114, 2146, 2511, 2543
Alw21I	GWGCWC	2 cut(s)	2424, 2575
Alw26I	GTCTC	5 cut(s)	651, 1711, 1805, 1827, 2100
AlwI	GGATC	6 cut(s)	217, 275, 663, 676, 2087, 2265
AlwNI	CAGNNNCTG	2 cut(s)	1337, 1961
Ama87I	CYCGRG	2 cut(s)	510, 972
AoxI	GGCC	4 cut(s)	18, 504, 1040, 2593
ApeKI	GCWGC	7 cut(s)	230, 839, 1025, 1171, 1246, 1349, 2783
ApoI	RAATTY	3 cut(s)	293, 1178, 1636
ArsI	GACNNNNNNTTYG	6 cut(s)	1295, 1327, 1991, 2023, 2511, 2543
AseI	ATTAAT	2 cut(s)	834, 947
Asp700I	GAANNNNTTC	1 cut(s)	2216
AspLEI	GCGC	3 cut(s)	1246, 1308, 2583
AsuC2I	CCSGG	2 cut(s)	385, 1400
AsuHPI	GGTGA	4 cut(s)	467, 773, 908, 2051
AsuII	TTCGAA	1 cut(s)	2690
AsuNHI	GCTAGC	1 cut(s)	2396
AvaI	CYCGRG	2 cut(s)	510, 972
BalI	TGGCCA	2 cut(s)	1042, 2595
BamHI	GGATCC	1 cut(s)	668
BanI	GGYRCC	2 cut(s)	1319, 2580
BanII	GRGCYC	1 cut(s)	2424
BarI	GAAGNNNNNNTAC	2 cut(s)	2204, 2236
BbsI	GAAGAC	2 cut(s)	191, 2437
Bbv12I	GWGCWC	2 cut(s)	2424, 2575
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	7 cut(s)	217, 826, 1037, 1183, 1258, 1336, 2795
BciT130I	CCWGG	4 cut(s)	738, 1830, 1956, 2372
BciVI	GTATCC	1 cut(s)	1458
BclI	TGATCA	2 cut(s)	193, 2452
BcnI	CCSGG	2 cut(s)	385, 1400
BcoDI	GTCTC	5 cut(s)	651, 1711, 1805, 1827, 2100
BcuI	ACTAGT	2 cut(s)	1109, 2044
BfmI	CTRYAG	3 cut(s)	840, 1368, 2301
BfoI	RGCGCY	2 cut(s)	1309, 2584
BfuI	GTATCC	1 cut(s)	1458
BglI	GCCNNNNNGGC	1 cut(s)	1328
BlpI	GCTNAGC	1 cut(s)	573
BmcAI	AGTACT	2 cut(s)	200, 2801
Bme1390I	CCNGG	6 cut(s)	385, 738, 1400, 1830, 1956, 2372
BmeRI	GACNNNNNGTC	2 cut(s)	471, 2045
BmeT110I	CYCGRG	2 cut(s)	510, 972
BmiI	GGNNCC	8 cut(s)	507, 670, 1321, 1398, 2282, 2582, 2964, 3001
BmrFI	CCNGG	6 cut(s)	385, 738, 1400, 1830, 1956, 2372
BmrI	ACTGGG	2 cut(s)	1113, 2945
BmsI	GCATC	7 cut(s)	1003, 1066, 1219, 1246, 1540, 2343, 2684
BmtI	GCTAGC	1 cut(s)	2400
BmuI	ACTGGG	2 cut(s)	1113, 2945
BpiI	GAAGAC	2 cut(s)	191, 2437
BpmI	CTGGAG	3 cut(s)	1851, 2409, 2438
Bpu10I	CCTNAGC	1 cut(s)	22
Bpu1102I	GCTNAGC	1 cut(s)	573
Bpu14I	TTCGAA	1 cut(s)	2690
BpuEI	CTTGAG	1 cut(s)	1610
BpuMI	CCSGG	2 cut(s)	385, 1400
BsaBI	GATNNNNATC	1 cut(s)	1554
BsaHI	GRCGYC	1 cut(s)	2581
BsaI	GGTCTC	2 cut(s)	1805, 1827
BsaJI	CCNNGG	6 cut(s)	736, 1222, 1455, 1576, 2343, 3003
BsaXI	ACNNNNNCTCC	2 cut(s)	1890, 1920
Bse3DI	GCAATG	1 cut(s)	1394
Bse8I	GATNNNNATC	1 cut(s)	1554
BseBI	CCWGG	4 cut(s)	738, 1830, 1956, 2372
BseDI	CCNNGG	6 cut(s)	736, 1222, 1455, 1576, 2343, 3003
BseGI	GGATG	7 cut(s)	182, 524, 768, 1517, 1884, 2746, 2916
BseJI	GATNNNNATC	1 cut(s)	1554
BseMI	GCAATG	1 cut(s)	1394
BseRI	GAGGAG	1 cut(s)	2406
BseXI	GCAGC	7 cut(s)	217, 826, 1037, 1183, 1258, 1336, 2795
BsgI	GTGCAG	1 cut(s)	572
Bsh1236I	CGCG	2 cut(s)	1244, 2120
BshFI	GGCC	4 cut(s)	20, 506, 1042, 2595
BshNI	GGYRCC	2 cut(s)	1319, 2580
BsiHKAI	GWGCWC	2 cut(s)	2424, 2575
BsiHKCI	CYCGRG	2 cut(s)	510, 972
BsiSI	CCGG	2 cut(s)	384, 1400
BslFI	GGGAC	4 cut(s)	1674, 2266, 2777, 2976
BsmAI	GTCTC	5 cut(s)	651, 1711, 1805, 1827, 2100
BsmFI	GGGAC	4 cut(s)	1674, 2266, 2777, 2976
BsmI	GAATGC	1 cut(s)	222
BsnI	GGCC	4 cut(s)	20, 506, 1042, 2595
Bso31I	GGTCTC	2 cut(s)	1805, 1827
BsoBI	CYCGRG	2 cut(s)	510, 972
Bsp119I	TTCGAA	1 cut(s)	2690
Bsp1286I	GDGCHC	2 cut(s)	2424, 2575
Bsp1407I	TGTACA	1 cut(s)	592
Bsp1720I	GCTNAGC	1 cut(s)	573
Bsp19I	CCATGG	1 cut(s)	3003
BspACI	CCGC	8 cut(s)	90, 388, 546, 1079, 1163, 2120, 2306, 2705
BspANI	GGCC	4 cut(s)	20, 506, 1042, 2595
BspFNI	CGCG	2 cut(s)	1244, 2120
BspHI	TCATGA	1 cut(s)	823
BspLI	GGNNCC	8 cut(s)	507, 670, 1321, 1398, 2282, 2582, 2964, 3001
BspMAI	CTGCAG	1 cut(s)	844
BspOI	GCTAGC	1 cut(s)	2400
BspPI	GGATC	6 cut(s)	217, 275, 663, 676, 2087, 2265
BspT104I	TTCGAA	1 cut(s)	2690
BspT107I	GGYRCC	2 cut(s)	1319, 2580
BspTNI	GGTCTC	2 cut(s)	1805, 1827
BsrDI	GCAATG	1 cut(s)	1394
BsrGI	TGTACA	1 cut(s)	592
BssECI	CCNNGG	6 cut(s)	736, 1222, 1455, 1576, 2343, 3003
BssNI	GRCGYC	1 cut(s)	2581
BssT1I	CCWWGG	3 cut(s)	1222, 2343, 3003
Bst2UI	CCWGG	4 cut(s)	738, 1830, 1956, 2372
Bst4CI	ACNGT	7 cut(s)	190, 346, 728, 1599, 1665, 2488, 2527
Bst6I	CTCTTC	4 cut(s)	2164, 2733, 2900, 2988
BstACI	GRCGYC	1 cut(s)	2581
BstAPI	GCANNNNNTGC	1 cut(s)	1022
BstAUI	TGTACA	1 cut(s)	592
BstBI	TTCGAA	1 cut(s)	2690
BstC8I	GCNNGC	4 cut(s)	419, 555, 2118, 2398
BstDSI	CCRYGG	1 cut(s)	3003
BstEII	GGTNACC	1 cut(s)	473
BstF5I	GGATG	7 cut(s)	182, 524, 768, 1517, 1884, 2746, 2916
BstFNI	CGCG	2 cut(s)	1244, 2120
BstH2I	RGCGCY	2 cut(s)	1309, 2584
BstHHI	GCGC	3 cut(s)	1246, 1308, 2583
BstMAI	GTCTC	5 cut(s)	651, 1711, 1805, 1827, 2100
BstMWI	GCNNNNNNNGC	8 cut(s)	311, 512, 554, 845, 1022, 1328, 1559, 2434
BstNI	CCWGG	4 cut(s)	738, 1830, 1956, 2372
BstNSI	RCATGY	3 cut(s)	237, 421, 438
BstPI	GGTNACC	1 cut(s)	473
BstSCI	CCNGG	6 cut(s)	383, 736, 1398, 1828, 1954, 2370
BstSFI	CTRYAG	3 cut(s)	840, 1368, 2301
BstUI	CGCG	2 cut(s)	1244, 2120
BstV1I	GCAGC	7 cut(s)	217, 826, 1037, 1183, 1258, 1336, 2795
BstV2I	GAAGAC	2 cut(s)	191, 2437
BstX2I	RGATCY	3 cut(s)	668, 2079, 2270
BstXI	CCANNNNNNTGG	1 cut(s)	1725
BstYI	RGATCY	3 cut(s)	668, 2079, 2270
BsuI	GTATCC	1 cut(s)	1458
BsuRI	GGCC	4 cut(s)	20, 506, 1042, 2595
BtgI	CCRYGG	1 cut(s)	3003
BtsCI	GGATG	7 cut(s)	182, 524, 768, 1517, 1884, 2746, 2916
BtsI	GCAGTG	1 cut(s)	19
BtsIMutI	CAGTG	9 cut(s)	19, 1043, 1351, 1424, 1716, 2223, 2385, 2493, 2717
Cac8I	GCNNGC	4 cut(s)	419, 555, 2118, 2398
CaiI	CAGNNNCTG	2 cut(s)	1337, 1961
CciI	TCATGA	1 cut(s)	823
CfoI	GCGC	3 cut(s)	1246, 1308, 2583
CseI	GACGC	1 cut(s)	977
Csp6I	GTAC	9 cut(s)	103, 199, 593, 907, 988, 1907, 2605, 2753, 2800
CspCI	CAANNNNNGTGG	4 cut(s)	311, 346, 2601, 2636
CviQI	GTAC	9 cut(s)	103, 199, 593, 907, 988, 1907, 2605, 2753, 2800
DinI	GGCGCC	1 cut(s)	2582
DriI	GACNNNNNGTC	2 cut(s)	471, 2045
EaeI	YGGCCR	2 cut(s)	1040, 2593
Eam1104I	CTCTTC	4 cut(s)	2164, 2733, 2900, 2988
Eam1105I	GACNNNNNGTC	2 cut(s)	471, 2045
EarI	CTCTTC	4 cut(s)	2164, 2733, 2900, 2988
Ecl136II	GAGCTC	1 cut(s)	2422
Eco130I	CCWWGG	3 cut(s)	1222, 2343, 3003
Eco24I	GRGCYC	1 cut(s)	2424
Eco31I	GGTCTC	2 cut(s)	1805, 1827
Eco53kI	GAGCTC	1 cut(s)	2422
Eco57I	CTGAAG	5 cut(s)	1145, 1859, 2714, 2808, 2846
Eco88I	CYCGRG	2 cut(s)	510, 972
Eco91I	GGTNACC	1 cut(s)	473
EcoICRI	GAGCTC	1 cut(s)	2422
EcoO109I	RGGNCCY	3 cut(s)	505, 1396, 2963
EcoO65I	GGTNACC	1 cut(s)	473
EcoRII	CCWGG	4 cut(s)	736, 1828, 1954, 2370
EcoT14I	CCWWGG	3 cut(s)	1222, 2343, 3003
EcoT22I	ATGCAT	2 cut(s)	423, 985
EcoT38I	GRGCYC	1 cut(s)	2424
EgeI	GGCGCC	1 cut(s)	2582
EheI	GGCGCC	1 cut(s)	2582
ErhI	CCWWGG	3 cut(s)	1222, 2343, 3003
FaqI	GGGAC	4 cut(s)	1674, 2266, 2777, 2976
FauI	CCCGC	1 cut(s)	2299
FbaI	TGATCA	2 cut(s)	193, 2452
FokI	GGATG	7 cut(s)	189, 531, 755, 1524, 1891, 2753, 2923
FriOI	GRGCYC	1 cut(s)	2424
GlaI	GCGC	3 cut(s)	1245, 1307, 2582
GsuI	CTGGAG	3 cut(s)	1851, 2409, 2438
HaeII	RGCGCY	2 cut(s)	1309, 2584
HaeIII	GGCC	4 cut(s)	20, 506, 1042, 2595
HapII	CCGG	2 cut(s)	384, 1400
HgaI	GACGC	1 cut(s)	977
HhaI	GCGC	3 cut(s)	1246, 1308, 2583
Hin1I	GRCGYC	1 cut(s)	2581
Hin6I	GCGC	3 cut(s)	1244, 1306, 2581
HinP1I	GCGC	3 cut(s)	1244, 1306, 2581
HincII	GTYRAC	4 cut(s)	336, 1084, 1200, 2293
HindII	GTYRAC	4 cut(s)	336, 1084, 1200, 2293
HpaI	GTTAAC	2 cut(s)	336, 2293
HpaII	CCGG	2 cut(s)	384, 1400
HphI	GGTGA	4 cut(s)	467, 773, 908, 2051
Hpy188I	TCNGA	9 cut(s)	285, 569, 696, 1012, 1125, 1327, 1948, 2275, 2899
Hpy99I	CGWCG	2 cut(s)	173, 1058
HpyAV	CCTTC	9 cut(s)	122, 1466, 2020, 2206, 2232, 2510, 2561, 2857, 2921
HpyCH4III	ACNGT	7 cut(s)	190, 346, 728, 1599, 1665, 2488, 2527
HpyCH4IV	ACGT	1 cut(s)	171
HpyF10VI	GCNNNNNNNGC	8 cut(s)	311, 512, 554, 845, 1022, 1328, 1559, 2434
HpySE526I	ACGT	1 cut(s)	171
Hsp92I	GRCGYC	1 cut(s)	2581
HspAI	GCGC	3 cut(s)	1244, 1306, 2581
KasI	GGCGCC	1 cut(s)	2580
Ksp22I	TGATCA	2 cut(s)	193, 2452
KspAI	GTTAAC	2 cut(s)	336, 2293
LmnI	GCTCC	4 cut(s)	490, 569, 2393, 2419
Lsp1109I	GCAGC	7 cut(s)	217, 826, 1037, 1183, 1258, 1336, 2795
LweI	GCATC	7 cut(s)	1003, 1066, 1219, 1246, 1540, 2343, 2684
MaeII	ACGT	1 cut(s)	171
MaeIII	GTNAC	8 cut(s)	71, 473, 761, 859, 1261, 1292, 1618, 2089
MflI	RGATCY	3 cut(s)	668, 2079, 2270
MhlI	GDGCHC	2 cut(s)	2424, 2575
MlsI	TGGCCA	2 cut(s)	1042, 2595
MluNI	TGGCCA	2 cut(s)	1042, 2595
Mly113I	GGCGCC	1 cut(s)	2581
MlyI	GAGTC	4 cut(s)	248, 794, 929, 1997
MmeI	TCCRAC	9 cut(s)	154, 187, 231, 1475, 1706, 1897, 2298, 2718, 2877
Mox20I	TGGCCA	2 cut(s)	1042, 2595
Mph1103I	ATGCAT	2 cut(s)	423, 985
MroXI	GAANNNNTTC	1 cut(s)	2216
MscI	TGGCCA	2 cut(s)	1042, 2595
MslI	CAYNNNNRTG	6 cut(s)	162, 449, 1254, 2186, 2436, 2775
Msp20I	TGGCCA	2 cut(s)	1042, 2595
MspA1I	CMGCKG	2 cut(s)	548, 2786
MspI	CCGG	2 cut(s)	384, 1400
MspR9I	CCNGG	6 cut(s)	385, 738, 1400, 1830, 1956, 2372
Mva1269I	GAATGC	1 cut(s)	222
MvaI	CCWGG	4 cut(s)	738, 1830, 1956, 2372
MvnI	CGCG	2 cut(s)	1244, 2120
MwoI	GCNNNNNNNGC	8 cut(s)	311, 512, 554, 845, 1022, 1328, 1559, 2434
NarI	GGCGCC	1 cut(s)	2581
NciI	CCSGG	2 cut(s)	385, 1400
NcoI	CCATGG	1 cut(s)	3003
NheI	GCTAGC	1 cut(s)	2396
NlaIV	GGNNCC	8 cut(s)	507, 670, 1321, 1398, 2282, 2582, 2964, 3001
NmuCI	GTSAC	6 cut(s)	473, 761, 859, 1261, 1292, 2089
NsiI	ATGCAT	2 cut(s)	423, 985
NspI	RCATGY	3 cut(s)	237, 421, 438
NspV	TTCGAA	1 cut(s)	2690
PaeI	GCATGC	1 cut(s)	421
PaeR7I	CTCGAG	1 cut(s)	972
PagI	TCATGA	1 cut(s)	823
PcsI	WCGNNNNNNNCGW	1 cut(s)	2581
PctI	GAATGC	1 cut(s)	222
PdmI	GAANNNNTTC	1 cut(s)	2216
PfeI	GAWTC	9 cut(s)	266, 587, 1239, 1571, 1886, 1912, 2056, 2228, 2687
PflFI	GACNNNGTC	1 cut(s)	257
PflMI	CCANNNNNTGG	3 cut(s)	1689, 1721, 1961
PleI	GAGTC	4 cut(s)	248, 794, 928, 1996
PluTI	GGCGCC	1 cut(s)	2584
PpsI	GAGTC	4 cut(s)	248, 794, 928, 1996
PpuMI	RGGWCCY	2 cut(s)	1396, 2963
PshBI	ATTAAT	2 cut(s)	834, 947
PsiI	TTATAA	1 cut(s)	1500
Psp124BI	GAGCTC	1 cut(s)	2424
Psp5II	RGGWCCY	2 cut(s)	1396, 2963
Psp6I	CCWGG	4 cut(s)	736, 1828, 1954, 2370
PspEI	GGTNACC	1 cut(s)	473
PspGI	CCWGG	4 cut(s)	736, 1828, 1954, 2370
PspN4I	GGNNCC	8 cut(s)	507, 670, 1321, 1398, 2282, 2582, 2964, 3001
PspPPI	RGGWCCY	2 cut(s)	1396, 2963
PspXI	VCTCGAGB	1 cut(s)	972
PstI	CTGCAG	1 cut(s)	844
PstNI	CAGNNNCTG	2 cut(s)	1337, 1961
PsuI	RGATCY	3 cut(s)	668, 2079, 2270
PsyI	GACNNNGTC	1 cut(s)	257
PvuII	CAGCTG	1 cut(s)	2786
RsaI	GTAC	9 cut(s)	104, 200, 594, 908, 989, 1908, 2606, 2754, 2801
RsaNI	GTAC	9 cut(s)	103, 199, 593, 907, 988, 1907, 2605, 2753, 2800
RseI	CAYNNNNRTG	6 cut(s)	162, 449, 1254, 2186, 2436, 2775
SacI	GAGCTC	1 cut(s)	2424
ScaI	AGTACT	2 cut(s)	200, 2801
SchI	GAGTC	4 cut(s)	248, 794, 929, 1997
ScrFI	CCNGG	6 cut(s)	385, 738, 1400, 1830, 1956, 2372
SduI	GDGCHC	2 cut(s)	2424, 2575
SfaNI	GCATC	7 cut(s)	1003, 1066, 1219, 1246, 1540, 2343, 2684
SfcI	CTRYAG	3 cut(s)	840, 1368, 2301
SfoI	GGCGCC	1 cut(s)	2582
Sfr274I	CTCGAG	1 cut(s)	972
SfuI	TTCGAA	1 cut(s)	2690
SlaI	CTCGAG	1 cut(s)	972
SmiMI	CAYNNNNRTG	6 cut(s)	162, 449, 1254, 2186, 2436, 2775
SmlI	CTYRAG	2 cut(s)	972, 1589
SmoI	CTYRAG	2 cut(s)	972, 1589
SpeI	ACTAGT	2 cut(s)	1109, 2044
SphI	GCATGC	1 cut(s)	421
SsiI	CCGC	8 cut(s)	90, 388, 546, 1079, 1163, 2120, 2306, 2705
SspDI	GGCGCC	1 cut(s)	2580
SstI	GAGCTC	1 cut(s)	2424
StyD4I	CCNGG	6 cut(s)	383, 736, 1398, 1828, 1954, 2370
StyI	CCWWGG	3 cut(s)	1222, 2343, 3003
TaaI	ACNGT	7 cut(s)	190, 346, 728, 1599, 1665, 2488, 2527
TaiI	ACGT	1 cut(s)	174
TaqI	TCGA	8 cut(s)	903, 973, 2049, 2260, 2448, 2530, 2690, 2804
TaqII	GACCGA	2 cut(s)	2129, 2356
TatI	WGTACW	7 cut(s)	102, 198, 592, 987, 2604, 2752, 2799
TauI	GCSGC	3 cut(s)	391, 1166, 2707
TfiI	GAWTC	9 cut(s)	266, 587, 1239, 1571, 1886, 1912, 2056, 2228, 2687
TscAI	CASTG	9 cut(s)	19, 1050, 1351, 1424, 1716, 2230, 2392, 2493, 2724
TseFI	GTSAC	6 cut(s)	473, 761, 859, 1261, 1292, 2089
TseI	GCWGC	7 cut(s)	230, 839, 1025, 1171, 1246, 1349, 2783
Tsp45I	GTSAC	6 cut(s)	473, 761, 859, 1261, 1292, 2089
TspGWI	ACGGA	3 cut(s)	734, 1891, 2750
TspRI	CASTG	9 cut(s)	19, 1050, 1351, 1424, 1716, 2230, 2392, 2493, 2724
Tth111I	GACNNNGTC	1 cut(s)	257
Van91I	CCANNNNNTGG	3 cut(s)	1689, 1721, 1961
VspI	ATTAAT	2 cut(s)	834, 947
XapI	RAATTY	3 cut(s)	293, 1178, 1636
XceI	RCATGY	3 cut(s)	237, 421, 438
XcmI	CCANNNNNNNNNTGG	2 cut(s)	170, 1722
XhoI	CTCGAG	1 cut(s)	972
XmnI	GAANNNNTTC	1 cut(s)	2216
ZrmI	AGTACT	2 cut(s)	200, 2801
Zsp2I	ATGCAT	2 cut(s)	423, 985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.