Rroxscaffold_2G00143370

Lysosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004264
Physical Location & Seq
Reverse (-)
81416330 .. 81422443
6114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143370.1

Sequence Viewer

Length: 3087 bp
ATGAAGAAGGGCAGTGTGGCCTCAGCTTTTCATGTGCCTATTGTTGTTGTTGTTGTTATTGTTTGCTTGTGTAACAAATCTGTTAATGGGGGTTTTGTGAAGTACAACACAGGGAGTGGTGTGGTGGAAGGCAAACTAAATGTTCATTTGGTTGCGCACTCCCATGACGACGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAATAGCATTCAGGCTGCATGTATAGAGAATGTGTTGGACTCTGTCATGGAATCGTTGGTTCGTAATCCAAATAGGAAATTTGTGTTTGCTGAGATGTATGAACTTTTAGGCATTTTTCAAAGATGGTGGTTAACACAAACTGTGGAAATGCAAGAACAAGTGAGAAAGCTAGTAGAAGCCGGGCAGCTAGAGTTTGTGTATGCCTCTAGCAATCAACTTGTTTTGAAAACTCGTTTATTTGTGTTTTGGATTTTGGATCTGTTGTTAAGCCTTATGCAAATGCCTATGCATAGAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGACCAGACGACACTAGGTCACCGTGCAATCAAGGAGCAATTCAACAAGGCCCCCCGAGCAGGATGGCAAATCGATACATTTGGACACTCGGCTGTGCAGGCTTACTTGCTTGGAGCTGAGCTTGGATTTGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGAAAAGCGAAAGAGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTGTTCACTACGGTCCACCCCCAGGTTTCCGTTTTGAAATCAATGGTGACTTCATCCCTATTCAGGATAATCCTCTACTTTTTGACTCCAATGTTGAAAAGCGTGTTCATGATTTTATTAATGCTGCAGTCACACAGGCTAATGTGACAAGGACAGAACACATTATGTGGACAATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAAATGGACAAATTAATTCACTATGTTAATAAGGACGGTCGAGTTAATGCATTGTACTCTACCCCATCAATCTATTCTGATGCAAAAAATGCAGCAAATCAGTCATGGCCACTGAAAACCGATGATTATTTTCCTTATGCAGATGTGATCAACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTCTGAAGCGATATGTAAGAATGTTGAGTGGCTACTACTTGGCAGCAAGGCAACTAGAATTTCTGGTGGGAAAGAGATCAACTGGTCCAAATACTTTTAGCCTTGGAGATGCTTTAGGAATTGTGCAGCACCACGATGCAGTCACAGGCACTTCTAAACAGCACACAACAAGTGACTATGCAAAGCGCCTATCTATTGGTGCATCTGAGGCAGAAGCTGTTGTCAGTGCTGCTCTATCATGCCTAACTACAGTGTCAATTACTCAATATCAGTTATTGCCCTCCAACCGAGGAAGGGATACCAGAAGGAAAGAGTTTGTGGCTTATAATCCTCTTGGATGGAATAGAACTGAGAATGTTAGAATACCTGTTAATGATGCCAATCTCGCTGTCCAAGATTCCCTCGGTAATTCTCTTGAGGCACAGTTTGTAAGTTTGGATAATGTTACAATAAATATAAGAAACTTCTATACAAAAGCATACTTGGGACTGTCATCAAAACAAGTTCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCACCACTTGGTTGGAACACATACTTCATTTCTAGACGAGCTACCAGAGGAAAAGACAGAAATGGATTTTTTTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAATTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCCAACGGATGTATGATTCCAAAACAGGAGTTGATGTACCAATTCAGCAGAGCTTCCTTTGGTATAATTCAAGCTCAGAAATTCCAGGTTCTGGTGCATACATTTTTCGCCCCGAGTCCTTTCCAAAAATTGTGCCAAGATCAGTAAACTTGAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACCAGACTTTACAGGGACAAACAGCACGCGGAAGTTGAGTTCACTATCGGTCCAATTCCCACCGAAGATTATACTGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGAGAAGGTGTTCTACACTGATTCAAATGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGATCCGACTGGTCCCTTTTGGTTAACCAACCTGTAGCAGGAAATTATTATCCATTAAATCTTGGCATTTTTACCTCGGATAAGAAATATGAGTTTTCGGTCCTGGTAGATCGTGCAACTGGAGGAGCTAGCATTAAAGATGGAGAACTGGAGCTCATGCTTCATAGGCGTGTGCTCTTCGATGATCATAAAGGATTGGAGGAAGCCCTTGACGAAACAGTGTGTATCAATGGAAATAGTATATGTGAAGGACTAACGGTTCGAGGGAATTATTATGTGAACATTAACCAAGTAGGGGAAGGTGCTCGTTGGCGCCGAAAAACTGGCCAAGAAGTGTACTCTCCACTTGTTTTGGCTTTCACACATGAGAAATTGGAGAATTGGACTGCATCCCATTTGACAAAAGCATCTACAATGGATTTGAATTACACCTTGCCGCCCAATGTAGCGCTGATTACTCTTCAGGAGTTGGATGATGGCAGTGTACTTGTCCGTCTAGCACATCTATATGAGGCAGCTGAAGTCCCTGGGTACTCGACATTAACCAAAGTTGAACTGAAGAAGATGTTTACAGGAAAAATGATAAAGGAAGTGAAGGAGATGAGCTTGTCAGCAAACCAAGACAAGTCGGAGATGAAGAGGATGGAATGGAAAGTTGAAGGTAATGACAGAGAGCCTATCCCAGTTAGAGGGGGACCTGTAAATAATTCAACTCTTCTTGTTGAGCTTGGACCCATGGAGATACGAACTTTCTTGCTGAAGTTTTAG

Protein Analysis

1028

Amino Acids

116.69

Weight (kDa)

6.94

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_38N PF01074 47 - 140 5.1e-19 Glycosyl hydrolases family 38 N-terminal domain
Glyco_hydro_38N PF01074 171 - 394 3.7e-48 Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid PF09261 399 - 491 1.9e-18 Alpha mannosidase middle domain
Laman-like_dom PF21260 550 - 599 4.4e-07 Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C PF07748 629 - 836 9e-46 Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2 PF17677 921 - 1024 7e-08 Glycosyl hydrolases family 38 C-terminal beta sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1554
Acc16I TGCGCA 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 2631
AccB7I CCANNNNNTGG 3 cut(s) 1743, 1775, 2015
AccII CGCG 1 cut(s) 2171
AciI CCGC 2 cut(s) 2171, 2756
AclWI GGATC 6 cut(s) 217, 480, 771, 784, 2138, 2316
AcoI YGGCCR 2 cut(s) 1148, 2644
AcsI RAATTY 3 cut(s) 293, 1286, 2004
AcuI CTGAAG 5 cut(s) 1253, 1913, 2765, 2859, 2897
AcyI GRCGYC 1 cut(s) 2632
AfaI GTAC 9 cut(s) 104, 200, 702, 1016, 1097, 1962, 2657, 2805, 2852
AfeI AGCGCT 1 cut(s) 2769
AhdI GACNNNNNGTC 2 cut(s) 579, 2096
AhlI ACTAGT 2 cut(s) 1217, 2095
AjnI CCWGG 5 cut(s) 844, 1882, 2008, 2421, 2845
AjuI GAANNNNNNNTTGG 2 cut(s) 502, 534
AloI GAACNNNNNNTCC 4 cut(s) 2165, 2197, 2562, 2594
Alw21I GWGCWC 3 cut(s) 2475, 2496, 2626
Alw26I GTCTC 4 cut(s) 759, 1765, 1859, 1881
AlwI GGATC 6 cut(s) 217, 480, 771, 784, 2138, 2316
AlwNI CAGNNNCTG 2 cut(s) 1445, 2015
Ama87I CYCGRG 2 cut(s) 618, 2036
Aor51HI AGCGCT 1 cut(s) 2769
AoxI GGCC 4 cut(s) 18, 612, 1148, 2644
ApeKI GCWGC 8 cut(s) 230, 400, 947, 1133, 1271, 1354, 1457, 2834
ApoI RAATTY 3 cut(s) 293, 1286, 2004
ArsI GACNNNNNNTTYG 2 cut(s) 2562, 2594
AseI ATTAAT 2 cut(s) 942, 1055
Asp700I GAANNNNTTC 1 cut(s) 2267
AspLEI GCGC 4 cut(s) 157, 1416, 2634, 2770
AsuC2I CCSGG 1 cut(s) 397
AsuHPI GGTGA 5 cut(s) 575, 881, 1016, 2102, 2134
AsuNHI GCTAGC 1 cut(s) 2447
AvaI CYCGRG 2 cut(s) 618, 2036
AvaII GGWCC 9 cut(s) 836, 1313, 1880, 2090, 2192, 2331, 2419, 3014, 3050
BalI TGGCCA 2 cut(s) 1150, 2646
BamHI GGATCC 1 cut(s) 776
BanI GGYRCC 1 cut(s) 2631
BanII GRGCYC 1 cut(s) 2475
BarI GAAGNNNNNNTAC 2 cut(s) 2255, 2287
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 3 cut(s) 2475, 2496, 2626
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 8 cut(s) 217, 412, 934, 1145, 1283, 1366, 1444, 2846
BccI CCATC 9 cut(s) 171, 333, 621, 1114, 1560, 1840, 2453, 2789, 2956
BciT130I CCWGG 5 cut(s) 846, 1884, 2010, 2423, 2847
BciVI GTATCC 1 cut(s) 1519
BclI TGATCA 3 cut(s) 193, 1188, 2503
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 4 cut(s) 759, 1765, 1859, 1881
BcuI ACTAGT 2 cut(s) 1217, 2095
BfmI CTRYAG 3 cut(s) 948, 1476, 2352
BfoI RGCGCY 3 cut(s) 1417, 2635, 2771
BfuI GTATCC 1 cut(s) 1519
BisI GCNGC 9 cut(s) 231, 401, 948, 1134, 1272, 1355, 1458, 2756, 2835
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 9 cut(s) 232, 402, 949, 1135, 1273, 1356, 1459, 2757, 2836
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 6 cut(s) 397, 846, 1884, 2010, 2423, 2847
Bme18I GGWCC 9 cut(s) 836, 1313, 1880, 2090, 2192, 2331, 2419, 3014, 3050
BmeRI GACNNNNNGTC 2 cut(s) 579, 2096
BmeT110I CYCGRG 2 cut(s) 618, 2036
BmiI GGNNCC 6 cut(s) 615, 778, 2333, 2633, 3015, 3052
BmrFI CCNGG 6 cut(s) 397, 846, 1884, 2010, 2423, 2847
BmrI ACTGGG 2 cut(s) 1221, 2996
BmsI GCATC 7 cut(s) 1111, 1327, 1354, 1439, 1594, 2717, 2735
BmtI GCTAGC 1 cut(s) 2451
BmuI ACTGGG 2 cut(s) 1221, 2996
BpiI GAAGAC 1 cut(s) 191
BpmI CTGGAG 3 cut(s) 1905, 2460, 2489
Bpu10I CCTNAGC 1 cut(s) 22
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 1664
BpuMI CCSGG 1 cut(s) 397
Bsa29I ATCGAT 1 cut(s) 636
BsaBI GATNNNNATC 1 cut(s) 1608
BsaHI GRCGYC 1 cut(s) 2632
BsaI GGTCTC 2 cut(s) 1859, 1881
BsaJI CCNNGG 8 cut(s) 844, 1330, 1516, 1630, 2394, 2845, 2846, 3054
BsaXI ACNNNNNCTCC 2 cut(s) 1944, 1974
Bse1I ACTGG 9 cut(s) 1210, 1227, 1315, 2221, 2333, 2443, 2472, 2647, 3002
Bse8I GATNNNNATC 1 cut(s) 1608
BseBI CCWGG 5 cut(s) 846, 1884, 2010, 2423, 2847
BseCI ATCGAT 1 cut(s) 636
BseDI CCNNGG 8 cut(s) 844, 1330, 1516, 1630, 2394, 2845, 2846, 3054
BseGI GGATG 8 cut(s) 182, 632, 876, 1571, 1938, 2708, 2797, 2967
BseJI GATNNNNATC 1 cut(s) 1608
BseMII CTCAG 9 cut(s) 36, 297, 672, 815, 1017, 1425, 1569, 1776, 2013
BseNI ACTGG 9 cut(s) 1210, 1227, 1315, 2221, 2333, 2443, 2472, 2647, 3002
BseRI GAGGAG 1 cut(s) 2457
BseXI GCAGC 8 cut(s) 217, 412, 934, 1145, 1283, 1366, 1444, 2846
BsgI GTGCAG 2 cut(s) 680, 1373
Bsh1236I CGCG 1 cut(s) 2171
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 4 cut(s) 20, 614, 1150, 2646
BshNI GGYRCC 1 cut(s) 2631
BshVI ATCGAT 1 cut(s) 636
BsiEI CGRYCG 1 cut(s) 1081
BsiHKAI GWGCWC 3 cut(s) 2475, 2496, 2626
BsiHKCI CYCGRG 2 cut(s) 618, 2036
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 5 cut(s) 1728, 2171, 2317, 2828, 3027
BsmAI GTCTC 4 cut(s) 759, 1765, 1859, 1881
BsmFI GGGAC 5 cut(s) 1728, 2171, 2317, 2828, 3027
BsmI GAATGC 1 cut(s) 222
BsnI GGCC 4 cut(s) 20, 614, 1150, 2646
Bso31I GGTCTC 2 cut(s) 1859, 1881
BsoBI CYCGRG 2 cut(s) 618, 2036
Bsp1286I GDGCHC 3 cut(s) 2475, 2496, 2626
Bsp1407I TGTACA 1 cut(s) 700
Bsp1720I GCTNAGC 1 cut(s) 681
Bsp19I CCATGG 1 cut(s) 3054
BspACI CCGC 2 cut(s) 2171, 2756
BspANI GGCC 4 cut(s) 20, 614, 1150, 2646
BspCNI CTCAG 9 cut(s) 35, 298, 673, 814, 1018, 1426, 1570, 1775, 2012
BspDI ATCGAT 1 cut(s) 636
BspFNI CGCG 1 cut(s) 2171
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 6 cut(s) 615, 778, 2333, 2633, 3015, 3052
BspMAI CTGCAG 1 cut(s) 952
BspOI GCTAGC 1 cut(s) 2451
BspPI GGATC 6 cut(s) 217, 480, 771, 784, 2138, 2316
BspQI GCTCTTC 1 cut(s) 2501
BspT107I GGYRCC 1 cut(s) 2631
BspTNI GGTCTC 2 cut(s) 1859, 1881
BsrGI TGTACA 1 cut(s) 700
BsrI ACTGG 9 cut(s) 1210, 1227, 1315, 2221, 2333, 2443, 2472, 2647, 3002
BssECI CCNNGG 8 cut(s) 844, 1330, 1516, 1630, 2394, 2845, 2846, 3054
BssNI GRCGYC 1 cut(s) 2632
BssT1I CCWWGG 2 cut(s) 1330, 3054
Bst2UI CCWGG 5 cut(s) 846, 1884, 2010, 2423, 2847
Bst6I CTCTTC 5 cut(s) 2215, 2501, 2784, 2951, 3039
BstACI GRCGYC 1 cut(s) 2632
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstAUI TGTACA 1 cut(s) 700
BstC8I GCNNGC 4 cut(s) 527, 663, 2169, 2449
BstDEI CTNAG 9 cut(s) 22, 306, 681, 801, 1026, 1434, 1578, 1762, 1999
BstDSI CCRYGG 1 cut(s) 3054
BstEII GGTNACC 2 cut(s) 581, 2140
BstENI CCTNNNNNAGG 1 cut(s) 2355
BstF5I GGATG 8 cut(s) 182, 632, 876, 1571, 1938, 2708, 2797, 2967
BstFNI CGCG 1 cut(s) 2171
BstH2I RGCGCY 3 cut(s) 1417, 2635, 2771
BstHHI GCGC 4 cut(s) 157, 1416, 2634, 2770
BstMAI GTCTC 4 cut(s) 759, 1765, 1859, 1881
BstMCI CGRYCG 1 cut(s) 1081
BstMWI GCNNNNNNNGC 6 cut(s) 620, 662, 1130, 1436, 1613, 2485
BstNI CCWGG 5 cut(s) 846, 1884, 2010, 2423, 2847
BstNSI RCATGY 3 cut(s) 237, 529, 546
BstPI GGTNACC 2 cut(s) 581, 2140
BstSCI CCNGG 6 cut(s) 395, 844, 1882, 2008, 2421, 2845
BstSFI CTRYAG 3 cut(s) 948, 1476, 2352
BstUI CGCG 1 cut(s) 2171
BstV1I GCAGC 8 cut(s) 217, 412, 934, 1145, 1283, 1366, 1444, 2846
BstV2I GAAGAC 1 cut(s) 191
BstX2I RGATCY 4 cut(s) 472, 776, 2130, 2321
BstXI CCANNNNNNTGG 1 cut(s) 1779
BstYI RGATCY 4 cut(s) 472, 776, 2130, 2321
Bsu15I ATCGAT 1 cut(s) 636
BsuI GTATCC 1 cut(s) 1519
BsuRI GGCC 4 cut(s) 20, 614, 1150, 2646
BsuTUI ATCGAT 1 cut(s) 636
BtgI CCRYGG 1 cut(s) 3054
BtsCI GGATG 8 cut(s) 182, 632, 876, 1571, 1938, 2708, 2797, 2967
BtsI GCAGTG 2 cut(s) 19, 2806
BtsIMutI CAGTG 8 cut(s) 19, 1151, 1459, 1485, 1770, 2274, 2544, 2806
Cac8I GCNNGC 4 cut(s) 527, 663, 2169, 2449
CaiI CAGNNNCTG 2 cut(s) 1445, 2015
CciI TCATGA 1 cut(s) 931
CfoI GCGC 4 cut(s) 157, 1416, 2634, 2770
ClaI ATCGAT 1 cut(s) 636
Csp6I GTAC 9 cut(s) 103, 199, 701, 1015, 1096, 1961, 2656, 2804, 2851
CspCI CAANNNNNGTGG 4 cut(s) 323, 358, 2652, 2687
CviQI GTAC 9 cut(s) 103, 199, 701, 1015, 1096, 1961, 2656, 2804, 2851
DdeI CTNAG 9 cut(s) 22, 306, 681, 801, 1026, 1434, 1578, 1762, 1999
DinI GGCGCC 1 cut(s) 2633
DriI GACNNNNNGTC 2 cut(s) 579, 2096
EaeI YGGCCR 2 cut(s) 1148, 2644
Eam1104I CTCTTC 5 cut(s) 2215, 2501, 2784, 2951, 3039
Eam1105I GACNNNNNGTC 2 cut(s) 579, 2096
EarI CTCTTC 5 cut(s) 2215, 2501, 2784, 2951, 3039
Ecl136II GAGCTC 1 cut(s) 2473
Eco130I CCWWGG 2 cut(s) 1330, 3054
Eco24I GRGCYC 1 cut(s) 2475
Eco31I GGTCTC 2 cut(s) 1859, 1881
Eco47I GGWCC 9 cut(s) 836, 1313, 1880, 2090, 2192, 2331, 2419, 3014, 3050
Eco47III AGCGCT 1 cut(s) 2769
Eco53kI GAGCTC 1 cut(s) 2473
Eco57I CTGAAG 5 cut(s) 1253, 1913, 2765, 2859, 2897
Eco88I CYCGRG 2 cut(s) 618, 2036
Eco91I GGTNACC 2 cut(s) 581, 2140
EcoICRI GAGCTC 1 cut(s) 2473
EcoNI CCTNNNNNAGG 1 cut(s) 2355
EcoO109I RGGNCCY 2 cut(s) 613, 3014
EcoO65I GGTNACC 2 cut(s) 581, 2140
EcoRII CCWGG 5 cut(s) 844, 1882, 2008, 2421, 2845
EcoT14I CCWWGG 2 cut(s) 1330, 3054
EcoT22I ATGCAT 3 cut(s) 507, 531, 1093
EcoT38I GRGCYC 1 cut(s) 2475
EgeI GGCGCC 1 cut(s) 2633
EheI GGCGCC 1 cut(s) 2633
ErhI CCWWGG 2 cut(s) 1330, 3054
FaqI GGGAC 5 cut(s) 1728, 2171, 2317, 2828, 3027
FbaI TGATCA 3 cut(s) 193, 1188, 2503
Fnu4HI GCNGC 9 cut(s) 231, 401, 948, 1134, 1272, 1355, 1458, 2756, 2835
FokI GGATG 8 cut(s) 189, 639, 863, 1578, 1945, 2695, 2804, 2974
FriOI GRGCYC 1 cut(s) 2475
Fsp4HI GCNGC 9 cut(s) 231, 401, 948, 1134, 1272, 1355, 1458, 2756, 2835
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 4 cut(s) 156, 1415, 2633, 2769
GluI GCNGC 9 cut(s) 231, 401, 948, 1134, 1272, 1355, 1458, 2756, 2835
GsuI CTGGAG 3 cut(s) 1905, 2460, 2489
HaeII RGCGCY 3 cut(s) 1417, 2635, 2771
HaeIII GGCC 4 cut(s) 20, 614, 1150, 2646
HapII CCGG 1 cut(s) 396
HhaI GCGC 4 cut(s) 157, 1416, 2634, 2770
Hin1I GRCGYC 1 cut(s) 2632
Hin6I GCGC 4 cut(s) 155, 1414, 2632, 2768
HinP1I GCGC 4 cut(s) 155, 1414, 2632, 2768
HincII GTYRAC 2 cut(s) 348, 2344
HindII GTYRAC 2 cut(s) 348, 2344
HpaI GTTAAC 2 cut(s) 348, 2344
HpaII CCGG 1 cut(s) 396
HphI GGTGA 5 cut(s) 575, 881, 1016, 2102, 2134
Hpy188I TCNGA 8 cut(s) 804, 1120, 1233, 1435, 2002, 2326, 2398, 2950
Hpy99I CGWCG 1 cut(s) 173
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 6 cut(s) 620, 662, 1130, 1436, 1613, 2485
HpyF3I CTNAG 9 cut(s) 22, 306, 681, 801, 1026, 1434, 1578, 1762, 1999
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 2632
HspAI GCGC 4 cut(s) 155, 1414, 2632, 2768
KasI GGCGCC 1 cut(s) 2631
Ksp22I TGATCA 3 cut(s) 193, 1188, 2503
KspAI GTTAAC 2 cut(s) 348, 2344
LguI GCTCTTC 1 cut(s) 2501
LmnI GCTCC 4 cut(s) 598, 677, 2444, 2470
Lsp1109I GCAGC 8 cut(s) 217, 412, 934, 1145, 1283, 1366, 1444, 2846
LweI GCATC 7 cut(s) 1111, 1327, 1354, 1439, 1594, 2717, 2735
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 9 cut(s) 71, 581, 869, 952, 967, 1369, 1400, 1672, 2140
MflI RGATCY 4 cut(s) 472, 776, 2130, 2321
MhlI GDGCHC 3 cut(s) 2475, 2496, 2626
MlsI TGGCCA 2 cut(s) 1150, 2646
MluNI TGGCCA 2 cut(s) 1150, 2646
Mly113I GGCGCC 1 cut(s) 2632
MlyI GAGTC 4 cut(s) 248, 902, 1037, 2048
MmeI TCCRAC 9 cut(s) 154, 187, 231, 1536, 1760, 1951, 2349, 2769, 2928
Mox20I TGGCCA 2 cut(s) 1150, 2646
Mph1103I ATGCAT 3 cut(s) 507, 531, 1093
MroXI GAANNNNTTC 1 cut(s) 2267
MscI TGGCCA 2 cut(s) 1150, 2646
MslI CAYNNNNRTG 7 cut(s) 162, 510, 557, 1362, 2237, 2487, 2826
Msp20I TGGCCA 2 cut(s) 1150, 2646
MspA1I CMGCKG 1 cut(s) 2837
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 6 cut(s) 397, 846, 1884, 2010, 2423, 2847
Mva1269I GAATGC 1 cut(s) 222
MvaI CCWGG 5 cut(s) 846, 1884, 2010, 2423, 2847
MvnI CGCG 1 cut(s) 2171
MwoI GCNNNNNNNGC 6 cut(s) 620, 662, 1130, 1436, 1613, 2485
NarI GGCGCC 1 cut(s) 2632
NciI CCSGG 1 cut(s) 397
NcoI CCATGG 1 cut(s) 3054
NheI GCTAGC 1 cut(s) 2447
NlaIV GGNNCC 6 cut(s) 615, 778, 2333, 2633, 3015, 3052
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 7 cut(s) 581, 869, 952, 967, 1369, 1400, 2140
NsbI TGCGCA 1 cut(s) 156
NsiI ATGCAT 3 cut(s) 507, 531, 1093
NspI RCATGY 3 cut(s) 237, 529, 546
PaeI GCATGC 1 cut(s) 529
PagI TCATGA 1 cut(s) 931
PasI CCCWGGG 1 cut(s) 2846
PciSI GCTCTTC 1 cut(s) 2501
PcsI WCGNNNNNNNCGW 1 cut(s) 2632
PctI GAATGC 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 2267
PfeI GAWTC 6 cut(s) 266, 695, 1625, 1940, 2107, 2279
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 3 cut(s) 1743, 1775, 2015
PkrI GCNGC 9 cut(s) 232, 402, 949, 1135, 1273, 1356, 1459, 2757, 2836
PleI GAGTC 4 cut(s) 248, 902, 1036, 2047
PluTI GGCGCC 1 cut(s) 2635
PpsI GAGTC 4 cut(s) 248, 902, 1036, 2047
PpuMI RGGWCCY 1 cut(s) 3014
PshBI ATTAAT 2 cut(s) 942, 1055
PsiI TTATAA 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 2475
Psp5II RGGWCCY 1 cut(s) 3014
Psp6I CCWGG 5 cut(s) 844, 1882, 2008, 2421, 2845
PspEI GGTNACC 2 cut(s) 581, 2140
PspGI CCWGG 5 cut(s) 844, 1882, 2008, 2421, 2845
PspN4I GGNNCC 6 cut(s) 615, 778, 2333, 2633, 3015, 3052
PspPPI RGGWCCY 1 cut(s) 3014
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 2 cut(s) 1445, 2015
PsuI RGATCY 4 cut(s) 472, 776, 2130, 2321
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 2837
RsaI GTAC 9 cut(s) 104, 200, 702, 1016, 1097, 1962, 2657, 2805, 2852
RsaNI GTAC 9 cut(s) 103, 199, 701, 1015, 1096, 1961, 2656, 2804, 2851
RseI CAYNNNNRTG 7 cut(s) 162, 510, 557, 1362, 2237, 2487, 2826
SacI GAGCTC 1 cut(s) 2475
SapI GCTCTTC 1 cut(s) 2501
SatI GCNGC 9 cut(s) 231, 401, 948, 1134, 1272, 1355, 1458, 2756, 2835
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 4 cut(s) 248, 902, 1037, 2048
ScrFI CCNGG 6 cut(s) 397, 846, 1884, 2010, 2423, 2847
SduI GDGCHC 3 cut(s) 2475, 2496, 2626
SfaNI GCATC 7 cut(s) 1111, 1327, 1354, 1439, 1594, 2717, 2735
SfcI CTRYAG 3 cut(s) 948, 1476, 2352
SfoI GGCGCC 1 cut(s) 2633
SinI GGWCC 9 cut(s) 836, 1313, 1880, 2090, 2192, 2331, 2419, 3014, 3050
SmiMI CAYNNNNRTG 7 cut(s) 162, 510, 557, 1362, 2237, 2487, 2826
SmlI CTYRAG 1 cut(s) 1643
SmoI CTYRAG 1 cut(s) 1643
SpeI ACTAGT 2 cut(s) 1217, 2095
SphI GCATGC 1 cut(s) 529
SsiI CCGC 2 cut(s) 2171, 2756
SspDI GGCGCC 1 cut(s) 2631
SstI GAGCTC 1 cut(s) 2475
StyD4I CCNGG 6 cut(s) 395, 844, 1882, 2008, 2421, 2845
StyI CCWWGG 2 cut(s) 1330, 3054
TaiI ACGT 1 cut(s) 174
TaqI TCGA 8 cut(s) 636, 1011, 1081, 2100, 2311, 2499, 2581, 2855
TaqII GACCGA 2 cut(s) 2180, 2407
TatI WGTACW 6 cut(s) 102, 198, 700, 1095, 2655, 2803
TauI GCSGC 1 cut(s) 2758
TfiI GAWTC 6 cut(s) 266, 695, 1625, 1940, 2107, 2279
TscAI CASTG 8 cut(s) 19, 1158, 1459, 1485, 1770, 2281, 2544, 2806
TseFI GTSAC 7 cut(s) 581, 869, 952, 967, 1369, 1400, 2140
TseI GCWGC 8 cut(s) 230, 400, 947, 1133, 1271, 1354, 1457, 2834
Tsp45I GTSAC 7 cut(s) 581, 869, 952, 967, 1369, 1400, 2140
TspGWI ACGGA 3 cut(s) 842, 1945, 2801
TspRI CASTG 8 cut(s) 19, 1158, 1459, 1485, 1770, 2281, 2544, 2806
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 3 cut(s) 1743, 1775, 2015
VpaK11BI GGWCC 9 cut(s) 836, 1313, 1880, 2090, 2192, 2331, 2419, 3014, 3050
VspI ATTAAT 2 cut(s) 942, 1055
XagI CCTNNNNNAGG 1 cut(s) 2355
XapI RAATTY 3 cut(s) 293, 1286, 2004
XbaI TCTAGA 1 cut(s) 1799
XceI RCATGY 3 cut(s) 237, 529, 546
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 1776
XmnI GAANNNNTTC 1 cut(s) 2267
ZrmI AGTACT 1 cut(s) 200
Zsp2I ATGCAT 3 cut(s) 507, 531, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.