AT5G67370

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007147
Physical Location & Seq
Reverse (-)
26877272 .. 26879639
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67370.1

Sequence Viewer

Length: 984 bp
ATGCTCAGGTTAATCGTTAATTACCCTCTGATTCCCAAGATATCCCACCGGGTTTGTAGTAACAGTAGTAGTAAGCTCGGGAGTTACTATGATTCTTCGTCGATCATAAAATATGGCGGAATCAGCGATGTTGTTGGTAAGAAGCAGGAGCTTTTTCTAAGTGTTTCGGTGAAAGCAGTGGAAGACAAAGGTAACAATGGAGGTGGAAGCATGAGTTTCTCGGGTCAAAGCTGGGATCCTAGTTCCGAGATTGAAGTTCCTTCAGACCAAAGACCCGTGAATGAGTATTCGTCTCTAAAGGAAGGAATGTTGTATTCATGGGGAGAATTGGGTCCAAGCGAATTCTTTATTCGTCTAGGTGGTCTTTGGTTGGTCACTTTCACTGTTCTTGGAGTTCCTGTTGCAGCTGCTAGCTTTAATCCTTCCAGAGAGCCTTTGAGATTTATTCTAGCTGCAGGGACTGGAACTTTATTTCTCGTTTCTTTGATTGTCTTGAGAATTTATCTGGGGTGGAGCTATGTTGGAGATAGACTACTTTCTGCAGTTATACCTTACGAAGAGAGTGGATGGTATGATGGACAAATGTGGGTCAAACCACCTGAGGTGTTGGCTCGCGACAGGTTGTTGGGTTCTTACAAGGTGAAGCCAGTGATCAAGATGCTCAAACAAACATTAATCGGAACAGGAGCTTTACTCGTTTCAGCATTCGTGCTATTCGTTTTCGCAACACCAGTCGAGGATTTCTTTAAAACCACTCTAGGATCTACAGAAAACCAACCAGAAGTTTCCATTTCAAGAACCAGCAACAAGTTCAATATAAGAAAAGAACAATTGCTTCGACTACCCGTAGATGTTGTGACTGACGATGATCTGGCAGCAGCCGCGGCTGAGGCTGCGGATGGAAGACCTGTCTATTGCAGAGACCGGTACTACCGTGCATTAGCAGGTGGTCAATACTGCAAATGGGAAGATCTTGTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

327

Amino Acids

36.11

Weight (kDa)

7.6

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 85 - 226 1.3e-56 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 935
Acc36I ACCTGC 1 cut(s) 935
AccII CGCG 2 cut(s) 615, 884
AciI CCGC 4 cut(s) 117, 882, 884, 896
AclWI GGATC 3 cut(s) 230, 243, 769
AcsI RAATTY 2 cut(s) 341, 498
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 929
AgeI ACCGGT 1 cut(s) 924
AgsI TTSAA 3 cut(s) 254, 795, 814
AluBI AGCT 8 cut(s) 76, 151, 231, 407, 414, 452, 516, 689
AluI AGCT 8 cut(s) 76, 151, 231, 407, 414, 452, 516, 689
Alw26I GTCTC 2 cut(s) 297, 915
AlwI GGATC 3 cut(s) 230, 243, 769
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 2 cut(s) 77, 220
ApeKI GCWGC 6 cut(s) 404, 407, 452, 875, 878, 893
ApoI RAATTY 2 cut(s) 341, 498
AseI ATTAAT 1 cut(s) 674
AsiGI ACCGGT 1 cut(s) 924
AspS9I GGNCC 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 181, 652
AsuNHI GCTAGC 1 cut(s) 410
AvaI CYCGRG 2 cut(s) 77, 220
AvaII GGWCC 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 600
BaeI ACNNNNGTAYC 2 cut(s) 919, 952
BamHI GGATCC 1 cut(s) 235
BbsI GAAGAC 2 cut(s) 189, 910
BbvCI CCTCAGC 1 cut(s) 888
BbvI GCAGC 6 cut(s) 394, 416, 439, 880, 887, 890
BccI CCATC 3 cut(s) 561, 569, 893
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 297, 915
BfaI CTAG 5 cut(s) 240, 356, 411, 449, 758
BfmI CTRYAG 3 cut(s) 453, 540, 765
BfuAI ACCTGC 1 cut(s) 935
BglII AGATCT 1 cut(s) 970
BisI GCNGC 8 cut(s) 405, 408, 453, 876, 879, 882, 885, 894
BlsI GCNGC 8 cut(s) 406, 409, 454, 877, 880, 883, 886, 895
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 332
BmeT110I CYCGRG 2 cut(s) 77, 220
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 237, 333
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 648
BmtI GCTAGC 1 cut(s) 414
BpiI GAAGAC 2 cut(s) 189, 910
BplI GAGNNNNNCTC 2 cut(s) 678, 710
Bpu10I CCTNAGC 2 cut(s) 5, 888
BpuEI CTTGAG 1 cut(s) 514
BpuMI CCSGG 1 cut(s) 50
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 882
BsaWI WCCGGW 1 cut(s) 924
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bse118I RCCGGY 1 cut(s) 924
Bse1I ACTGG 3 cut(s) 466, 647, 731
Bse21I CCTNAGG 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 882
BseGI GGATG 2 cut(s) 572, 904
BseMII CTCAG 3 cut(s) 19, 591, 879
BseNI ACTGG 3 cut(s) 466, 647, 731
BseXI GCAGC 6 cut(s) 394, 416, 439, 880, 887, 890
BseYI CCCAGC 1 cut(s) 231
Bsh1236I CGCG 2 cut(s) 615, 884
BshTI ACCGGT 1 cut(s) 924
BsiHKCI CYCGRG 2 cut(s) 77, 220
BsiSI CCGG 2 cut(s) 49, 925
BslFI GGGAC 1 cut(s) 472
BsmAI GTCTC 2 cut(s) 297, 915
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 704
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 2 cut(s) 77, 220
Bsp143I GATC 6 cut(s) 102, 235, 651, 761, 868, 970
Bsp68I TCGCGA 1 cut(s) 615
BspACI CCGC 4 cut(s) 117, 882, 884, 896
BspCNI CTCAG 3 cut(s) 18, 592, 880
BspFNI CGCG 2 cut(s) 615, 884
BspLI GGNNCC 2 cut(s) 237, 333
BspMAI CTGCAG 2 cut(s) 457, 544
BspMI ACCTGC 1 cut(s) 935
BspOI GCTAGC 1 cut(s) 414
BspPI GGATC 3 cut(s) 230, 243, 769
BspTNI GGTCTC 1 cut(s) 915
BsrFI RCCGGY 1 cut(s) 924
BsrI ACTGG 3 cut(s) 466, 647, 731
BssAI RCCGGY 1 cut(s) 924
BssECI CCNNGG 1 cut(s) 882
BssMI GATC 6 cut(s) 102, 235, 651, 761, 868, 970
Bst4CI ACNGT 3 cut(s) 65, 385, 935
Bst6I CTCTTC 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 412, 613
BstDEI CTNAG 4 cut(s) 5, 158, 600, 888
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 2 cut(s) 572, 904
BstFNI CGCG 2 cut(s) 615, 884
BstKTI GATC 6 cut(s) 105, 238, 654, 764, 871, 973
BstMAI GTCTC 2 cut(s) 297, 915
BstMBI GATC 6 cut(s) 102, 235, 651, 761, 868, 970
BstMWI GCNNNNNNNGC 5 cut(s) 123, 881, 884, 890, 893
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 3 cut(s) 453, 540, 765
BstUI CGCG 2 cut(s) 615, 884
BstV1I GCAGC 6 cut(s) 394, 416, 439, 880, 887, 890
BstV2I GAAGAC 2 cut(s) 189, 910
BstX2I RGATCY 3 cut(s) 235, 761, 970
BstYI RGATCY 3 cut(s) 235, 761, 970
Bsu36I CCTNAGG 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 882
BtgZI GCGATG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 572, 904
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 3 cut(s) 183, 381, 654
BtuMI TCGCGA 1 cut(s) 615
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 2 cut(s) 412, 613
CaiI CAGNNNCTG 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 924
Cfr13I GGNCC 1 cut(s) 332
Cfr42I CCGCGG 1 cut(s) 885
Csp6I GTAC 1 cut(s) 928
CspAI ACCGGT 1 cut(s) 924
CspCI CAANNNNNGTGG 4 cut(s) 35, 70, 184, 219
CviAII CATG 2 cut(s) 211, 318
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 4 cut(s) 5, 158, 600, 888
DpnI GATC 6 cut(s) 104, 237, 653, 763, 870, 972
DpnII GATC 6 cut(s) 102, 235, 651, 761, 868, 970
DraI TTTAAA 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 915
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 600
Eco88I CYCGRG 2 cut(s) 77, 220
EcoRI GAATTC 1 cut(s) 341
EcoRV GATATC 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 2 cut(s) 214, 321
FaiI YATR 9 cut(s) 90, 107, 114, 212, 319, 519, 548, 573, 818
FaqI GGGAC 1 cut(s) 472
FatI CATG 2 cut(s) 210, 317
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 8 cut(s) 405, 408, 453, 876, 879, 882, 885, 894
FokI GGATG 2 cut(s) 579, 911
Fsp4HI GCNGC 8 cut(s) 405, 408, 453, 876, 879, 882, 885, 894
FspBI CTAG 5 cut(s) 240, 356, 411, 449, 758
GluI GCNGC 8 cut(s) 405, 408, 453, 876, 879, 882, 885, 894
GsaI CCCAGC 1 cut(s) 235
HapII CCGG 2 cut(s) 49, 925
Hin1II CATG 2 cut(s) 214, 321
HinfI GANTC 3 cut(s) 31, 92, 120
HpaII CCGG 2 cut(s) 49, 925
HphI GGTGA 2 cut(s) 181, 652
Hpy188I TCNGA 4 cut(s) 30, 247, 265, 680
Hpy188III TCNNGA 6 cut(s) 79, 426, 493, 614, 655, 795
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 3 cut(s) 270, 296, 432
HpyCH4III ACNGT 3 cut(s) 65, 385, 935
HpyCH4V TGCA 6 cut(s) 404, 455, 542, 918, 938, 960
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 881, 884, 890, 893
HpyF3I CTNAG 4 cut(s) 5, 158, 600, 888
Hsp92II CATG 2 cut(s) 214, 321
Ksp22I TGATCA 1 cut(s) 651
KspI CCGCGG 1 cut(s) 885
Kzo9I GATC 6 cut(s) 102, 235, 651, 761, 868, 970
LmnI GCTCC 3 cut(s) 148, 513, 686
Lsp1109I GCAGC 6 cut(s) 394, 416, 439, 880, 887, 890
LweI GCATC 1 cut(s) 648
MaeI CTAG 5 cut(s) 240, 356, 411, 449, 758
MaeIII GTNAC 5 cut(s) 59, 83, 191, 373, 856
MalI GATC 6 cut(s) 104, 237, 653, 763, 870, 972
MboI GATC 6 cut(s) 102, 235, 651, 761, 868, 970
MboII GAAGA 5 cut(s) 87, 194, 569, 915, 980
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 3 cut(s) 235, 761, 970
MluCI AATT 5 cut(s) 19, 326, 341, 498, 830
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 5 cut(s) 36, 194, 595, 730, 883
MseI TTAA 6 cut(s) 11, 18, 417, 674, 747, 978
MspA1I CMGCKG 2 cut(s) 407, 884
MspI CCGG 2 cut(s) 49, 925
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 704
MvnI CGCG 2 cut(s) 615, 884
MwoI GCNNNNNNNGC 5 cut(s) 123, 881, 884, 890, 893
NciI CCSGG 1 cut(s) 50
NdeII GATC 6 cut(s) 102, 235, 651, 761, 868, 970
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 2 cut(s) 214, 321
NlaIV GGNNCC 2 cut(s) 237, 333
NmuCI GTSAC 2 cut(s) 373, 856
NruI TCGCGA 1 cut(s) 615
PaqCI CACCTGC 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 714
PctI GAATGC 1 cut(s) 704
PfeI GAWTC 3 cut(s) 31, 92, 120
PinAI ACCGGT 1 cut(s) 924
PkrI GCNGC 8 cut(s) 406, 409, 454, 877, 880, 883, 886, 895
PshBI ATTAAT 1 cut(s) 674
PspFI CCCAGC 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 237, 333
PspPI GGNCC 1 cut(s) 332
PstI CTGCAG 2 cut(s) 457, 544
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 3 cut(s) 235, 761, 970
PvuII CAGCTG 1 cut(s) 407
RruI TCGCGA 1 cut(s) 615
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
SacII CCGCGG 1 cut(s) 885
SaqAI TTAA 6 cut(s) 11, 18, 417, 674, 747, 978
SatI GCNGC 8 cut(s) 405, 408, 453, 876, 879, 882, 885, 894
Sau3AI GATC 6 cut(s) 102, 235, 651, 761, 868, 970
Sau96I GGNCC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 50
SfaNI GCATC 1 cut(s) 648
SfcI CTRYAG 3 cut(s) 453, 540, 765
Sfr303I CCGCGG 1 cut(s) 885
SgrBI CCGCGG 1 cut(s) 885
SinI GGWCC 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 5 cut(s) 19, 326, 341, 498, 830
SsiI CCGC 4 cut(s) 117, 882, 884, 896
SspMI CTAG 5 cut(s) 240, 356, 411, 449, 758
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 65, 385, 935
TaqI TCGA 3 cut(s) 101, 735, 838
TasI AATT 5 cut(s) 19, 326, 341, 498, 830
TauI GCSGC 2 cut(s) 884, 887
TfiI GAWTC 3 cut(s) 31, 92, 120
Tru1I TTAA 6 cut(s) 11, 18, 417, 674, 747, 978
Tru9I TTAA 6 cut(s) 11, 18, 417, 674, 747, 978
TscAI CASTG 3 cut(s) 183, 388, 654
TseFI GTSAC 2 cut(s) 373, 856
TseI GCWGC 6 cut(s) 404, 407, 452, 875, 878, 893
Tsp45I GTSAC 2 cut(s) 373, 856
TspDTI ATGAA 1 cut(s) 306
TspRI CASTG 3 cut(s) 183, 388, 654
VpaK11BI GGWCC 1 cut(s) 332
VspI ATTAAT 1 cut(s) 674
XapI RAATTY 2 cut(s) 341, 498
XspI CTAG 5 cut(s) 240, 356, 411, 449, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.