MD15G1293200.v1.1

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007147
Physical Location & Seq
Reverse (-)
27171916 .. 27176523
4608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1293200.v1.1.491

Sequence Viewer

Length: 942 bp
ATGCTCCGAGCGCAAGTGTATTGCTCTCTGCTCTTTAGTCCTCCAAAAGTCAAGTTAGTGACGGTTGGTAGCAGCTCCACCTCATGGCTCATCCAATACCGTAGTGCTAGAAGGCTGCCCAAGGGGGTTTCTATCAAGGCCTTGAAAGATGGGGCAGGCGGGGAAACGAGTGGCTTCCGCGGGCAGAGTTGGGATCCGGGGTCAGAAATTGAGGTTCCTTTTGAGCAAAGGCCGGTGAATGAGTACTCATCTCTGAAGGACGGAGCCTTGTACTCGTGGGGCGAACTGGGACCTGGGCCCTTTTTACTTCGACTCGGAGGTTTGTGGTTGGTAACTTTCACAACTCTAGGATTGCCTATTGCTGCTGCAAGCTTTAATCCATCAAGGGACCCTCTAAGATTTGCGCTAGCCGCTGGAACTGGAACTCTTTTTGTTGTATCGTTGATTGTCTTAAGAATTTATCTGGGATGGAGTTATGTTGGTGATAGGCTACTATCAGCAGTCATACCTTATGAAGAGAGTGGCTGGTATGACGGACAAATGTGGGTAAAGCCCCCTGAGGTCTTGGCTCGTGACAGACTCTTGGGATCTTACAAGGTCAAACCAGTTATCAAATTGCTGAAACAGACTCTAGTTGGAACAGGGGCATTACTTGTTACAGGAGTGCTGCTGTTTATCTTTGCTACACCAGTGGAAGACTTTGTTCGCACCACATTTGGCACGAAACAAAACTCGTCAGATGTTTCCGCCTCAAAAATCAGCTCAAAGTTCAATATAAGTAAAGAGGAGTTACTTAAACTGCCTATGGATGTGAAGGCCAATGATGATCTAGCAGCAGCCGCTGCTGAGGCTGCTGACGGAAGACCAGTCTACTGCAGAGATCGGTTTTATCGTGCATTAGCAGGTGGACAATACTGCAAATGGGAGGATCTGCTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

314

Amino Acids

34.29

Weight (kDa)

9.28

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 71 - 212 1.2e-53 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 893
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 870
AccII CGCG 1 cut(s) 180
AciI CCGC 6 cut(s) 159, 178, 180, 411, 747, 840
AclWI GGATC 4 cut(s) 188, 201, 595, 936
AcsI RAATTY 1 cut(s) 456
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 2 cut(s) 245, 272
AfiI CCNNNNNNNGG 1 cut(s) 84
AflII CTTAAG 2 cut(s) 451, 935
AgsI TTSAA 2 cut(s) 145, 772
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 3 cut(s) 75, 372, 762
AluI AGCT 3 cut(s) 75, 372, 762
AlwI GGATC 4 cut(s) 188, 201, 595, 936
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 4 cut(s) 138, 230, 296, 816
ApaI GGGCCC 1 cut(s) 300
ApeKI GCWGC 9 cut(s) 72, 115, 362, 365, 667, 833, 836, 842, 851
ApoI RAATTY 1 cut(s) 456
AspLEI GCGC 2 cut(s) 13, 406
AspS9I GGNCC 4 cut(s) 290, 296, 297, 388
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 247, 494
AsuNHI GCTAGC 1 cut(s) 406
AvaII GGWCC 2 cut(s) 290, 388
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 300
BamHI GGATCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 300
BauI CACGAG 2 cut(s) 274, 570
BbsI GAAGAC 2 cut(s) 702, 868
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 9 cut(s) 84, 102, 349, 352, 654, 829, 838, 845, 848
BccI CCATC 3 cut(s) 143, 388, 462
BciT130I CCWGG 1 cut(s) 294
BcnI CCSGG 1 cut(s) 198
BfaI CTAG 5 cut(s) 108, 347, 407, 632, 830
BfmI CTRYAG 1 cut(s) 874
BfrI CTTAAG 2 cut(s) 451, 935
BfuAI ACCTGC 1 cut(s) 893
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 2 cut(s) 198, 294
Bme18I GGWCC 2 cut(s) 290, 388
BmgT120I GGNCC 4 cut(s) 290, 296, 297, 388
BmiI GGNNCC 7 cut(s) 195, 216, 265, 291, 298, 389, 390
BmrFI CCNGG 2 cut(s) 198, 294
BmrI ACTGGG 1 cut(s) 296
BmtI GCTAGC 1 cut(s) 410
BmuI ACTGGG 1 cut(s) 296
BpiI GAAGAC 2 cut(s) 702, 868
Bpu10I CCTNAGC 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 120, 178, 197, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 5 cut(s) 291, 424, 605, 689, 866
Bse21I CCTNAGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 120, 178, 197, 293
BseGI GGATG 3 cut(s) 90, 473, 814
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMII CTCAG 2 cut(s) 549, 837
BseNI ACTGG 5 cut(s) 291, 424, 605, 689, 866
BseRI GAGGAG 1 cut(s) 800
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 9 cut(s) 84, 102, 349, 352, 654, 829, 838, 845, 848
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 140, 232, 298, 818
BsiSI CCGG 2 cut(s) 197, 233
BslFI GGGAC 2 cut(s) 303, 401
BslI CCNNNNNNNGG 1 cut(s) 84
BsmFI GGGAC 2 cut(s) 303, 401
BsnI GGCC 4 cut(s) 140, 232, 298, 818
Bsp120I GGGCCC 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 5 cut(s) 193, 587, 826, 880, 928
BspACI CCGC 6 cut(s) 159, 178, 180, 411, 747, 840
BspANI GGCC 4 cut(s) 140, 232, 298, 818
BspCNI CTCAG 2 cut(s) 550, 838
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 7 cut(s) 195, 216, 265, 291, 298, 389, 390
BspMAI CTGCAG 1 cut(s) 878
BspMI ACCTGC 1 cut(s) 893
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 4 cut(s) 188, 201, 595, 936
BspTI CTTAAG 2 cut(s) 451, 935
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 5 cut(s) 291, 424, 605, 689, 866
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 4 cut(s) 120, 178, 197, 293
BssMI GATC 5 cut(s) 193, 587, 826, 880, 928
BssSI CACGAG 2 cut(s) 274, 570
BssT1I CCWWGG 1 cut(s) 120
Bst2BI CACGAG 2 cut(s) 274, 570
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 64, 101
Bst6I CTCTTC 1 cut(s) 510
BstAFI CTTAAG 2 cut(s) 451, 935
BstAPI GCANNNNNTGC 1 cut(s) 842
BstC8I GCNNGC 4 cut(s) 157, 182, 370, 408
BstDEI CTNAG 3 cut(s) 395, 558, 846
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 3 cut(s) 90, 473, 814
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 2 cut(s) 13, 406
BstKTI GATC 5 cut(s) 196, 590, 829, 883, 931
BstMBI GATC 5 cut(s) 193, 587, 826, 880, 928
BstMWI GCNNNNNNNGC 6 cut(s) 10, 410, 839, 842, 848, 851
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 2 cut(s) 196, 292
BstSFI CTRYAG 1 cut(s) 874
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 9 cut(s) 84, 102, 349, 352, 654, 829, 838, 845, 848
BstV2I GAAGAC 2 cut(s) 702, 868
BstX2I RGATCY 3 cut(s) 193, 587, 928
BstYI RGATCY 3 cut(s) 193, 587, 928
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 4 cut(s) 140, 232, 298, 818
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 90, 473, 814
BtsIMutI CAGTG 1 cut(s) 696
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 4 cut(s) 157, 182, 370, 408
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 13, 406
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 4 cut(s) 290, 296, 297, 388
Cfr42I CCGCGG 1 cut(s) 181
Csp6I GTAC 2 cut(s) 244, 271
CviAII CATG 1 cut(s) 84
CviQI GTAC 2 cut(s) 244, 271
DdeI CTNAG 3 cut(s) 395, 558, 846
DpnI GATC 5 cut(s) 195, 589, 828, 882, 930
DpnII GATC 5 cut(s) 193, 587, 826, 880, 928
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
EciI GGCGGA 1 cut(s) 736
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 290, 388
Eco57I CTGAAG 1 cut(s) 275
Eco81I CCTNAGG 1 cut(s) 558
EcoO109I RGGNCCY 3 cut(s) 290, 297, 388
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 87
FaiI YATR 7 cut(s) 85, 477, 506, 513, 531, 776, 806
FaqI GGGAC 2 cut(s) 303, 401
FatI CATG 1 cut(s) 83
FauI CCCGC 2 cut(s) 152, 173
FblI GTMKAC 1 cut(s) 870
FokI GGATG 3 cut(s) 77, 480, 821
FriOI GRGCYC 1 cut(s) 300
FspBI CTAG 5 cut(s) 108, 347, 407, 632, 830
GlaI GCGC 2 cut(s) 12, 405
HaeIII GGCC 4 cut(s) 140, 232, 298, 818
HapII CCGG 2 cut(s) 197, 233
HhaI GCGC 2 cut(s) 13, 406
Hin1II CATG 1 cut(s) 87
Hin6I GCGC 2 cut(s) 11, 404
HinP1I GCGC 2 cut(s) 11, 404
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 3 cut(s) 312, 579, 628
HpaII CCGG 2 cut(s) 197, 233
HphI GGTGA 2 cut(s) 247, 494
Hpy166II GTNNAC 2 cut(s) 871, 908
Hpy188I TCNGA 5 cut(s) 8, 205, 255, 317, 739
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 2 cut(s) 871, 908
HpyAV CCTTC 3 cut(s) 105, 250, 808
HpyCH4III ACNGT 2 cut(s) 64, 101
HpyCH4V TGCA 4 cut(s) 368, 876, 896, 918
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 410, 839, 842, 848, 851
HpyF3I CTNAG 3 cut(s) 395, 558, 846
Hsp92II CATG 1 cut(s) 87
HspAI GCGC 2 cut(s) 11, 404
KflI GGGWCCC 1 cut(s) 388
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 5 cut(s) 193, 587, 826, 880, 928
LmnI GCTCC 3 cut(s) 9, 80, 263
Lsp1109I GCAGC 9 cut(s) 84, 102, 349, 352, 654, 829, 838, 845, 848
MaeI CTAG 5 cut(s) 108, 347, 407, 632, 830
MaeIII GTNAC 5 cut(s) 58, 331, 572, 655, 789
MalI GATC 5 cut(s) 195, 589, 828, 882, 930
MboI GATC 5 cut(s) 193, 587, 826, 880, 928
MboII GAAGA 3 cut(s) 527, 707, 873
MflI RGATCY 3 cut(s) 193, 587, 928
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 3 cut(s) 207, 456, 614
MlyI GAGTC 3 cut(s) 306, 573, 622
MmeI TCCRAC 1 cut(s) 616
MseI TTAA 4 cut(s) 375, 452, 795, 936
MspA1I CMGCKG 3 cut(s) 180, 413, 842
MspCI CTTAAG 2 cut(s) 451, 935
MspI CCGG 2 cut(s) 197, 233
MspR9I CCNGG 2 cut(s) 198, 294
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 6 cut(s) 10, 410, 839, 842, 848, 851
NciI CCSGG 1 cut(s) 198
NdeII GATC 5 cut(s) 193, 587, 826, 880, 928
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 1 cut(s) 87
NlaIV GGNNCC 7 cut(s) 195, 216, 265, 291, 298, 389, 390
NmuCI GTSAC 2 cut(s) 58, 572
PaqCI CACCTGC 1 cut(s) 893
PceI AGGCCT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PflMI CCANNNNNTGG 1 cut(s) 84
PleI GAGTC 3 cut(s) 306, 573, 622
PpsI GAGTC 3 cut(s) 306, 573, 622
PpuMI RGGWCCY 2 cut(s) 290, 388
Psp5II RGGWCCY 2 cut(s) 290, 388
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 7 cut(s) 195, 216, 265, 291, 298, 389, 390
PspOMI GGGCCC 1 cut(s) 296
PspPI GGNCC 4 cut(s) 290, 296, 297, 388
PspPPI RGGWCCY 2 cut(s) 290, 388
PstI CTGCAG 1 cut(s) 878
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 3 cut(s) 193, 587, 928
RsaI GTAC 2 cut(s) 245, 272
RsaNI GTAC 2 cut(s) 244, 271
SacII CCGCGG 1 cut(s) 181
SaqAI TTAA 4 cut(s) 375, 452, 795, 936
Sau3AI GATC 5 cut(s) 193, 587, 826, 880, 928
Sau96I GGNCC 4 cut(s) 290, 296, 297, 388
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 3 cut(s) 306, 573, 622
ScrFI CCNGG 2 cut(s) 198, 294
SduI GDGCHC 1 cut(s) 300
SfcI CTRYAG 1 cut(s) 874
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SinI GGWCC 2 cut(s) 290, 388
SmlI CTYRAG 2 cut(s) 451, 935
SmoI CTYRAG 2 cut(s) 451, 935
Sse9I AATT 3 cut(s) 207, 456, 614
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 6 cut(s) 159, 178, 180, 411, 747, 840
SspMI CTAG 5 cut(s) 108, 347, 407, 632, 830
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 2 cut(s) 196, 292
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 64, 101
TaqI TCGA 1 cut(s) 310
TasI AATT 3 cut(s) 207, 456, 614
TatI WGTACW 2 cut(s) 243, 270
TauI GCSGC 2 cut(s) 413, 842
Tru1I TTAA 4 cut(s) 375, 452, 795, 936
Tru9I TTAA 4 cut(s) 375, 452, 795, 936
TscAI CASTG 1 cut(s) 696
TseFI GTSAC 2 cut(s) 58, 572
TseI GCWGC 9 cut(s) 72, 115, 362, 365, 667, 833, 836, 842, 851
Tsp45I GTSAC 2 cut(s) 58, 572
TspDTI ATGAA 1 cut(s) 528
TspGWI ACGGA 3 cut(s) 276, 549, 873
TspRI CASTG 1 cut(s) 696
Van91I CCANNNNNTGG 1 cut(s) 84
Vha464I CTTAAG 2 cut(s) 451, 935
VpaK11BI GGWCC 2 cut(s) 290, 388
XapI RAATTY 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 870
XspI CTAG 5 cut(s) 108, 347, 407, 632, 830
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.