Rh2AG193000

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007147
Physical Location & Seq
Reverse (-)
19126467 .. 19130193
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG193000.1

Sequence Viewer

Length: 930 bp
ATGCTCCGGGTAGATGTGTATTGCTCTCTACTTTCTAGTCCCCGACAAGTCAAGTTGGTAAATGTCGGCAGCAGAAGTTTAGGGTCATGGCAGATCCAGCAGCGTAGCGCTCAAAGACTACCCAAGGGGATTTCCGTCAAGGCCGTGAATGATGGGACGGGAGGGGGAACAAGCGGATTTCGGGGTCAGAGCTGGGAGCCTGGGTCAGAAATTGAGGTTCCTTTTGAACAAAGACCGGTGAATGAGTACTCATCTTTGAAGGAAGGAACCTTGTACTCGTGGGGCGATCTGGGACCGGGGCCTTTTTTCCTTCGTCTTGGAGGTTTGTGGTTGGTAACATTCACAGTTTTAGGAGCGCCCATAGCAGCTGCAAGCTTTAATCCATCAAGGGACCCTCTACGATTTGTTCTAGCAGCTGGAACCGGAACTCTTTTCCTTGTATCATTGATCGTCTTAAGAATTTATCTGGGATGGAGTTACGTTGGCGATAGACTACTATCAGCAGTCATACCTTACGAAGAGACTGGATGGTATGATGGACAAATGTGGGTGAAACCACCTGAGGTCCTGGCTCGTGACAGACTTTTGGGCTCTTACAAGGTTAAACCAGTCATCAAGTTGCTGAAACAGACTCTAGTCGGAACAGGGGTATTGCTTGTTTCAGCAGTGATGCTATTCATCTTTGCTACACCAGTGGAGGATTTCGTTCAAACCACATTTTCTACAAAAGAAAACTCATCAAATGTTTCTGTCTCAAAACTGAGAAGAGAAGAATTGCTTAAATTGCCTACGGAAGTAAAATCTGATGATGACCTAGCAGCAGCCGCTGCTGAGGCGGCAGACGGAAGACCAGTCTACTGCAGAGATAGGTTTTATCGTGCACTAGCAGGTGGACAATACTGCAAGTCGGATGATCTACTGATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

309

Amino Acids

33.94

Weight (kDa)

9.02

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 72 - 213 1.4e-54 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 878
AccI GTMKAC 1 cut(s) 855
AciI CCGC 3 cut(s) 174, 825, 836
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 459
AfaI GTAC 2 cut(s) 248, 275
AfeI AGCGCT 1 cut(s) 109
AflII CTTAAG 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 235
AgsI TTSAA 3 cut(s) 227, 259, 710
AjnI CCWGG 2 cut(s) 199, 567
AluBI AGCT 4 cut(s) 192, 368, 375, 416
AluI AGCT 4 cut(s) 192, 368, 375, 416
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 2 cut(s) 515, 757
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 1 cut(s) 88
AlwNI CAGNNNCTG 1 cut(s) 827
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 2 cut(s) 141, 299
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 8 cut(s) 69, 100, 365, 368, 413, 818, 821, 827
ApoI RAATTY 1 cut(s) 459
AsiGI ACCGGT 1 cut(s) 235
AspLEI GCGC 2 cut(s) 110, 358
AspS9I GGNCC 4 cut(s) 293, 299, 391, 565
AsuC2I CCSGG 2 cut(s) 8, 297
AsuHPI GGTGA 2 cut(s) 250, 562
AvaII GGWCC 3 cut(s) 293, 391, 565
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 883
BanII GRGCYC 1 cut(s) 593
BauI CACGAG 2 cut(s) 277, 573
BbsI GAAGAC 1 cut(s) 853
Bbv12I GWGCWC 1 cut(s) 883
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 8 cut(s) 81, 112, 355, 377, 425, 814, 830, 833
BccI CCATC 5 cut(s) 146, 391, 465, 522, 530
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 2 cut(s) 201, 569
BcnI CCSGG 2 cut(s) 8, 297
BcoDI GTCTC 2 cut(s) 515, 757
BfaI CTAG 5 cut(s) 36, 410, 635, 815, 884
BfmI CTRYAG 1 cut(s) 859
BfoI RGCGCY 2 cut(s) 111, 359
BfrI CTTAAG 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 878
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 4 cut(s) 8, 201, 297, 569
Bme18I GGWCC 3 cut(s) 293, 391, 565
BmgT120I GGNCC 4 cut(s) 293, 299, 391, 565
BmiI GGNNCC 8 cut(s) 198, 219, 268, 294, 300, 392, 393, 421
BmrFI CCNGG 4 cut(s) 8, 201, 297, 569
BmsI GCATC 1 cut(s) 660
BoxI GACNNNNGTC 1 cut(s) 635
BpiI GAAGAC 1 cut(s) 853
Bpu10I CCTNAGC 1 cut(s) 831
BpuMI CCSGG 2 cut(s) 8, 297
BsaJI CCNNGG 3 cut(s) 123, 200, 296
BsaWI WCCGGW 2 cut(s) 235, 422
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 4 cut(s) 529, 608, 692, 851
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 201, 569
BseDI CCNNGG 3 cut(s) 123, 200, 296
BseGI GGATG 3 cut(s) 476, 533, 916
BseMII CTCAG 3 cut(s) 552, 752, 822
BseNI ACTGG 4 cut(s) 529, 608, 692, 851
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 8 cut(s) 81, 112, 355, 377, 425, 814, 830, 833
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 2 cut(s) 143, 301
BshTI ACCGGT 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 4 cut(s) 7, 236, 296, 423
BslFI GGGAC 4 cut(s) 24, 169, 306, 404
BsmAI GTCTC 2 cut(s) 515, 757
BsmFI GGGAC 4 cut(s) 24, 169, 306, 404
BsnI GGCC 2 cut(s) 143, 301
Bsp1286I GDGCHC 2 cut(s) 593, 883
Bsp143I GATC 4 cut(s) 93, 286, 447, 913
BspACI CCGC 3 cut(s) 174, 825, 836
BspANI GGCC 2 cut(s) 143, 301
BspCNI CTCAG 3 cut(s) 553, 753, 823
BspLI GGNNCC 8 cut(s) 198, 219, 268, 294, 300, 392, 393, 421
BspMAI CTGCAG 1 cut(s) 863
BspMI ACCTGC 1 cut(s) 878
BspPI GGATC 1 cut(s) 88
BspTI CTTAAG 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 4 cut(s) 529, 608, 692, 851
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 123, 200, 296
BssMI GATC 4 cut(s) 93, 286, 447, 913
BssSI CACGAG 2 cut(s) 277, 573
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 2 cut(s) 277, 573
Bst2UI CCWGG 2 cut(s) 201, 569
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 2 cut(s) 513, 760
BstAFI CTTAAG 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 561, 761, 831
BstF5I GGATG 3 cut(s) 476, 533, 916
BstH2I RGCGCY 2 cut(s) 111, 359
BstHHI GCGC 2 cut(s) 110, 358
BstKTI GATC 4 cut(s) 96, 289, 450, 916
BstMAI GTCTC 2 cut(s) 515, 757
BstMBI GATC 4 cut(s) 93, 286, 447, 913
BstMWI GCNNNNNNNGC 7 cut(s) 97, 362, 784, 824, 827, 833, 836
BstNI CCWGG 2 cut(s) 201, 569
BstPAI GACNNNNGTC 1 cut(s) 635
BstSCI CCNGG 4 cut(s) 6, 199, 295, 567
BstSFI CTRYAG 1 cut(s) 859
BstSLI GKGCMC 1 cut(s) 883
BstV1I GCAGC 8 cut(s) 81, 112, 355, 377, 425, 814, 830, 833
BstV2I GAAGAC 1 cut(s) 853
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 2 cut(s) 143, 301
BtsCI GGATG 3 cut(s) 476, 533, 916
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 672, 699
BveI ACCTGC 1 cut(s) 878
Cac8I GCNNGC 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 2 cut(s) 110, 358
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 4 cut(s) 293, 299, 391, 565
Csp6I GTAC 2 cut(s) 247, 274
CspAI ACCGGT 1 cut(s) 235
CviAII CATG 1 cut(s) 87
CviQI GTAC 2 cut(s) 247, 274
DdeI CTNAG 3 cut(s) 561, 761, 831
DpnI GATC 4 cut(s) 95, 288, 449, 915
DpnII GATC 4 cut(s) 93, 286, 447, 913
Eam1104I CTCTTC 2 cut(s) 513, 760
EarI CTCTTC 2 cut(s) 513, 760
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 593
Eco47I GGWCC 3 cut(s) 293, 391, 565
Eco47III AGCGCT 1 cut(s) 109
Eco81I CCTNAGG 1 cut(s) 561
EcoO109I RGGNCCY 3 cut(s) 299, 391, 565
EcoRII CCWGG 2 cut(s) 199, 567
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 1 cut(s) 90
FaiI YATR 4 cut(s) 88, 362, 509, 534
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 4 cut(s) 24, 169, 306, 404
FatI CATG 1 cut(s) 86
FblI GTMKAC 1 cut(s) 855
FokI GGATG 3 cut(s) 483, 540, 923
FriOI GRGCYC 1 cut(s) 593
FspBI CTAG 5 cut(s) 36, 410, 635, 815, 884
GlaI GCGC 2 cut(s) 109, 357
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 2 cut(s) 111, 359
HaeIII GGCC 2 cut(s) 143, 301
HapII CCGG 4 cut(s) 7, 236, 296, 423
HhaI GCGC 2 cut(s) 110, 358
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 2 cut(s) 108, 356
HinP1I GCGC 2 cut(s) 108, 356
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 1 cut(s) 631
HpaII CCGG 4 cut(s) 7, 236, 296, 423
HphI GGTGA 2 cut(s) 250, 562
Hpy166II GTNNAC 3 cut(s) 856, 881, 893
Hpy188I TCNGA 5 cut(s) 189, 208, 641, 805, 910
Hpy188III TCNNGA 1 cut(s) 575
Hpy8I GTNNAC 3 cut(s) 856, 881, 893
HpyAV CCTTC 3 cut(s) 253, 257, 320
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 4 cut(s) 371, 861, 881, 903
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 362, 784, 824, 827, 833, 836
HpyF3I CTNAG 3 cut(s) 561, 761, 831
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 2 cut(s) 108, 356
KflI GGGWCCC 1 cut(s) 391
Kzo9I GATC 4 cut(s) 93, 286, 447, 913
LmnI GCTCC 3 cut(s) 9, 196, 353
Lsp1109I GCAGC 8 cut(s) 81, 112, 355, 377, 425, 814, 830, 833
LweI GCATC 1 cut(s) 660
MaeI CTAG 5 cut(s) 36, 410, 635, 815, 884
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 334, 476, 575
MalI GATC 4 cut(s) 95, 288, 449, 915
MboI GATC 4 cut(s) 93, 286, 447, 913
MboII GAAGA 4 cut(s) 530, 777, 782, 858
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 593, 883
MluCI AATT 4 cut(s) 210, 459, 773, 782
MlyI GAGTC 1 cut(s) 625
MmeI TCCRAC 2 cut(s) 619, 888
MnlI CCTC 7 cut(s) 155, 208, 314, 405, 556, 691, 826
MseI TTAA 4 cut(s) 378, 455, 603, 780
MspA1I CMGCKG 3 cut(s) 368, 416, 827
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 4 cut(s) 7, 236, 296, 423
MspR9I CCNGG 4 cut(s) 8, 201, 297, 569
MvaI CCWGG 2 cut(s) 201, 569
MwoI GCNNNNNNNGC 7 cut(s) 97, 362, 784, 824, 827, 833, 836
NciI CCSGG 2 cut(s) 8, 297
NdeII GATC 4 cut(s) 93, 286, 447, 913
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 8 cut(s) 198, 219, 268, 294, 300, 392, 393, 421
NmuCI GTSAC 1 cut(s) 575
PaqCI CACCTGC 1 cut(s) 878
PinAI ACCGGT 1 cut(s) 235
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
PpuMI RGGWCCY 2 cut(s) 391, 565
PshAI GACNNNNGTC 1 cut(s) 635
Psp5II RGGWCCY 2 cut(s) 391, 565
Psp6I CCWGG 2 cut(s) 199, 567
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 199, 567
PspN4I GGNNCC 8 cut(s) 198, 219, 268, 294, 300, 392, 393, 421
PspPI GGNCC 4 cut(s) 293, 299, 391, 565
PspPPI RGGWCCY 2 cut(s) 391, 565
PstI CTGCAG 1 cut(s) 863
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 2 cut(s) 368, 416
RsaI GTAC 2 cut(s) 248, 275
RsaNI GTAC 2 cut(s) 247, 274
SaqAI TTAA 4 cut(s) 378, 455, 603, 780
Sau3AI GATC 4 cut(s) 93, 286, 447, 913
Sau96I GGNCC 4 cut(s) 293, 299, 391, 565
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 4 cut(s) 8, 201, 297, 569
SduI GDGCHC 2 cut(s) 593, 883
SfaNI GCATC 1 cut(s) 660
SfcI CTRYAG 1 cut(s) 859
SinI GGWCC 3 cut(s) 293, 391, 565
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 4 cut(s) 210, 459, 773, 782
SsiI CCGC 3 cut(s) 174, 825, 836
SspMI CTAG 5 cut(s) 36, 410, 635, 815, 884
StyD4I CCNGG 4 cut(s) 6, 199, 295, 567
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 483
TasI AATT 4 cut(s) 210, 459, 773, 782
TatI WGTACW 2 cut(s) 246, 273
TauI GCSGC 2 cut(s) 827, 839
Tru1I TTAA 4 cut(s) 378, 455, 603, 780
Tru9I TTAA 4 cut(s) 378, 455, 603, 780
TscAI CASTG 2 cut(s) 672, 699
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 8 cut(s) 69, 100, 365, 368, 413, 818, 821, 827
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 1 cut(s) 667
TspGWI ACGGA 3 cut(s) 124, 806, 858
TspRI CASTG 2 cut(s) 672, 699
Vha464I CTTAAG 1 cut(s) 454
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 3 cut(s) 293, 391, 565
XapI RAATTY 1 cut(s) 459
XmiI GTMKAC 1 cut(s) 855
XspI CTAG 5 cut(s) 36, 410, 635, 815, 884
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.