MD02G1181900.v1.1

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007147
Physical Location & Seq
Reverse (-)
16381278 .. 16385054
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1181900.v1.1.491

Sequence Viewer

Length: 699 bp
ATGCTCCGGGCGCAGGTGTATTGTTCTCTACTCTTTAGTCCTCCACAATTCAAGTTAGTGAAGGTTGGTAGCAACTCTAGCTCATGGCTAATCCAATACCGTAGTGCTCAGAAGCTGCCCAATGAGGTTTCTGTCAAGGCCTTGAAAGATGGGGTAGGTGGGGAAACGAGCGGCTTCCGCGGGCAGATACTCGCTCTGAAGGATGGAGTCTTGTACTCGTGGGGCGAACTGGGACCTGGGCCTTTTTTACTTCGACTTGGAGGTTTGTGGTTGGTAACCTTCACAACTCTAGGATTGCCTATTGCAGCTACAAGCTTCAATCCATCAAGGGACCCTCTAAGATTTGCGCTAGCAGCTGGAACTGGAACTCTTTTTGTTGTGTTGTTGATTGTCTTAAGAATTTATCTGGGATGGAGCTATATTGGTGATAGGTTACTATCAGCAGTTATACCTTACGAAAAGAGTGGCTGGTATGACGGACAAATGTGGGTAAAGCCACATGAGGTTAAACCGGTCATCAAATTGCTGAAGCAGACTCTAGTGGGAACAGGAGCATTACTTGTTACAGGAGTGCTGTTATTTATCTTTGCTACACCGGTGAAGGATTTCATTCACACCACATTCGGCACAAAACAAAACCCATCAAGTGTTTCCGCCTCAAAGATCAGCTCAAAGCTCAATATAAGGTTTTATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

233

Amino Acids

25.45

Weight (kDa)

10.04

Isoelectric Point (pI)

24.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 65 - 178 1.4e-33 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4
Acc36I ACCTGC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 1 cut(s) 180
AciI CCGC 4 cut(s) 171, 178, 180, 654
AcsI RAATTY 1 cut(s) 399
AcuI CTGAAG 2 cut(s) 218, 548
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 13
AflII CTTAAG 1 cut(s) 394
AgeI ACCGGT 2 cut(s) 511, 595
AgsI TTSAA 3 cut(s) 52, 145, 319
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 8 cut(s) 81, 115, 308, 315, 356, 417, 669, 676
AluI AGCT 8 cut(s) 81, 115, 308, 315, 356, 417, 669, 676
Alw21I GWGCWC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 2 cut(s) 138, 239
ApeKI GCWGC 3 cut(s) 115, 305, 353
ApoI RAATTY 1 cut(s) 399
AsiGI ACCGGT 2 cut(s) 511, 595
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 2 cut(s) 13, 349
AspS9I GGNCC 3 cut(s) 233, 239, 331
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 437, 610
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 2 cut(s) 233, 331
BauI CACGAG 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 102, 317, 365
BccI CCATC 5 cut(s) 143, 197, 331, 405, 649
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 8
BfaI CTAG 4 cut(s) 78, 290, 350, 539
BfrI CTTAAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 4 cut(s) 116, 172, 306, 354
BlsI GCNGC 4 cut(s) 117, 173, 307, 355
Bme1390I CCNGG 2 cut(s) 8, 237
Bme18I GGWCC 2 cut(s) 233, 331
BmgT120I GGNCC 3 cut(s) 233, 239, 331
BmiI GGNNCC 3 cut(s) 234, 332, 333
BmrFI CCNGG 2 cut(s) 8, 237
BmrI ACTGGG 1 cut(s) 239
BmtI GCTAGC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 178, 236
BsaWI WCCGGW 2 cut(s) 511, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 13
Bse118I RCCGGY 2 cut(s) 511, 595
Bse1I ACTGG 2 cut(s) 234, 367
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 2 cut(s) 178, 236
BseGI GGATG 2 cut(s) 208, 416
BseLI CCNNNNNNNGG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 122
BseNI ACTGG 2 cut(s) 234, 367
BseXI GCAGC 3 cut(s) 102, 317, 365
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 2 cut(s) 140, 241
BshTI ACCGGT 2 cut(s) 511, 595
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 3 cut(s) 7, 512, 596
BslFI GGGAC 2 cut(s) 246, 344
BslI CCNNNNNNNGG 1 cut(s) 13
BsmFI GGGAC 2 cut(s) 246, 344
BsnI GGCC 2 cut(s) 140, 241
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 1 cut(s) 663
BspACI CCGC 4 cut(s) 171, 178, 180, 654
BspANI GGCC 2 cut(s) 140, 241
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 234, 332, 333
BspMI ACCTGC 1 cut(s) 4
BspOI GCTAGC 1 cut(s) 353
BspTI CTTAAG 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 2 cut(s) 511, 595
BsrI ACTGG 2 cut(s) 234, 367
BssAI RCCGGY 2 cut(s) 511, 595
BssECI CCNNGG 2 cut(s) 178, 236
BssMI GATC 1 cut(s) 663
BssSI CACGAG 1 cut(s) 217
Bst2BI CACGAG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 394
BstC8I GCNNGC 2 cut(s) 182, 351
BstDEI CTNAG 2 cut(s) 108, 338
BstDSI CCRYGG 1 cut(s) 178
BstEII GGTNACC 1 cut(s) 274
BstF5I GGATG 2 cut(s) 208, 416
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 2 cut(s) 13, 349
BstKTI GATC 1 cut(s) 666
BstMBI GATC 1 cut(s) 663
BstMWI GCNNNNNNNGC 4 cut(s) 10, 78, 177, 353
BstNI CCWGG 1 cut(s) 237
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 6, 235
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 102, 317, 365
BsuRI GGCC 2 cut(s) 140, 241
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 2 cut(s) 208, 416
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 2 cut(s) 182, 351
CaiI CAGNNNCTG 1 cut(s) 115
CfoI GCGC 2 cut(s) 13, 349
Cfr10I RCCGGY 2 cut(s) 511, 595
Cfr13I GGNCC 3 cut(s) 233, 239, 331
Cfr42I CCGCGG 1 cut(s) 181
Csp6I GTAC 1 cut(s) 214
CspAI ACCGGT 2 cut(s) 511, 595
CviAII CATG 2 cut(s) 84, 500
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 2 cut(s) 108, 338
DpnI GATC 1 cut(s) 665
DpnII GATC 1 cut(s) 663
EciI GGCGGA 1 cut(s) 643
Eco147I AGGCCT 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 233, 331
Eco57I CTGAAG 2 cut(s) 218, 548
Eco91I GGTNACC 1 cut(s) 274
EcoO109I RGGNCCY 2 cut(s) 233, 331
EcoO65I GGTNACC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 235
FaeI CATG 2 cut(s) 87, 503
FaiI YATR 7 cut(s) 85, 420, 449, 474, 501, 683, 693
FaqI GGGAC 2 cut(s) 246, 344
FatI CATG 2 cut(s) 83, 499
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 4 cut(s) 116, 172, 306, 354
FokI GGATG 2 cut(s) 215, 423
Fsp4HI GCNGC 4 cut(s) 116, 172, 306, 354
FspBI CTAG 4 cut(s) 78, 290, 350, 539
GlaI GCGC 2 cut(s) 12, 348
GluI GCNGC 4 cut(s) 116, 172, 306, 354
HaeIII GGCC 2 cut(s) 140, 241
HapII CCGG 3 cut(s) 7, 512, 596
HhaI GCGC 2 cut(s) 13, 349
Hin1II CATG 2 cut(s) 87, 503
Hin6I GCGC 2 cut(s) 11, 347
HinP1I GCGC 2 cut(s) 11, 347
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 2 cut(s) 207, 535
HpaII CCGG 3 cut(s) 7, 512, 596
HphI GGTGA 2 cut(s) 437, 610
Hpy188I TCNGA 2 cut(s) 111, 198
HpyAV CCTTC 4 cut(s) 55, 193, 289, 595
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 78, 177, 353
HpyF3I CTNAG 2 cut(s) 108, 338
Hsp92II CATG 2 cut(s) 87, 503
HspAI GCGC 2 cut(s) 11, 347
KflI GGGWCCC 1 cut(s) 331
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 9, 414, 551
Lsp1109I GCAGC 3 cut(s) 102, 317, 365
MaeI CTAG 4 cut(s) 78, 290, 350, 539
MaeIII GTNAC 3 cut(s) 274, 432, 562
MalI GATC 1 cut(s) 665
MbiI CCGCTC 1 cut(s) 171
MboI GATC 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 3 cut(s) 47, 399, 521
MlyI GAGTC 2 cut(s) 216, 529
MnlI CCTC 6 cut(s) 51, 118, 254, 345, 496, 667
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 2 cut(s) 395, 507
MspA1I CMGCKG 2 cut(s) 180, 356
MspCI CTTAAG 1 cut(s) 394
MspI CCGG 3 cut(s) 7, 512, 596
MspR9I CCNGG 2 cut(s) 8, 237
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 10, 78, 177, 353
NciI CCSGG 1 cut(s) 8
NdeII GATC 1 cut(s) 663
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 2 cut(s) 87, 503
NlaIV GGNNCC 3 cut(s) 234, 332, 333
PaqCI CACCTGC 1 cut(s) 4
PceI AGGCCT 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 605
PinAI ACCGGT 2 cut(s) 511, 595
PkrI GCNGC 4 cut(s) 117, 173, 307, 355
PleI GAGTC 2 cut(s) 215, 529
PpsI GAGTC 2 cut(s) 215, 529
PpuMI RGGWCCY 2 cut(s) 233, 331
Psp5II RGGWCCY 2 cut(s) 233, 331
Psp6I CCWGG 1 cut(s) 235
PspEI GGTNACC 1 cut(s) 274
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 234, 332, 333
PspPI GGNCC 3 cut(s) 233, 239, 331
PspPPI RGGWCCY 2 cut(s) 233, 331
PstNI CAGNNNCTG 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacII CCGCGG 1 cut(s) 181
SaqAI TTAA 2 cut(s) 395, 507
SatI GCNGC 4 cut(s) 116, 172, 306, 354
Sau3AI GATC 1 cut(s) 663
Sau96I GGNCC 3 cut(s) 233, 239, 331
SchI GAGTC 2 cut(s) 216, 529
ScrFI CCNGG 2 cut(s) 8, 237
SduI GDGCHC 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 181
SgrAI CRCCGGYG 1 cut(s) 595
SgrBI CCGCGG 1 cut(s) 181
SinI GGWCC 2 cut(s) 233, 331
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 3 cut(s) 47, 399, 521
SseBI AGGCCT 1 cut(s) 140
SsiI CCGC 4 cut(s) 171, 178, 180, 654
SspMI CTAG 4 cut(s) 78, 290, 350, 539
StuI AGGCCT 1 cut(s) 140
StyD4I CCNGG 2 cut(s) 6, 235
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 1 cut(s) 253
TasI AATT 3 cut(s) 47, 399, 521
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 174
Tru1I TTAA 2 cut(s) 395, 507
Tru9I TTAA 2 cut(s) 395, 507
TseI GCWGC 3 cut(s) 115, 305, 353
TspDTI ATGAA 1 cut(s) 598
TspGWI ACGGA 1 cut(s) 492
Vha464I CTTAAG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 233, 331
XapI RAATTY 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 4 cut(s) 78, 290, 350, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.