Rh2BG205200

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007147
Physical Location & Seq
Reverse (-)
18976359 .. 18980068
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG205200.1

Sequence Viewer

Length: 684 bp
ATGCTCCGGGTAGATGTGTATTGCTCTCTACTTTCTAGTCCCCGACAGGTCAAGTTGGTAAATGTCGGCAGCAGAAGTTTAGGGTCATGGCAGATCCAGCAGCGTAGCGCTCAAAGACTACCCAAGGGGATTTCTGTCAAGGCCGTGAATGATGGGACGGGAGGGGGAACAAGCGGATTTCGGGGTCAGAGCTGGGAGCCTGGGTCAGAAATTGAGGTTCCTTTTGAACAAAGACCGGTGAATGAGTACTCATCTTTGAAGGAAGGAACCTTGTACTCGTGGGGCGATCTGGGACCAGGGCCTTTTTTCCTTCGTCTTGGAGGTTTGTGGTTGGTAACATTCACAGTTTTAGGAGCGCCCATAGCAGCTGCAAGCTTTAATCCATCAAGGGACCCTCTACGATTTGTTCTAGCAGCTGGAACCGGAACTCTTTTCCTTGTATCGTTGATCGTCTTAAGAATTTATCTGGGATGGAGTTACGTTGGCGATAGACTACTATCAGCAGTCATACCTTACGAAGAGACTGGATGGTATGATGGACAAATGTGGGTGAAACCACCTGAGGTAAGCACCTTTCCCATATTCTTTCTTATTGTGTTCACTGTTCAATTCATTTTTTGTTCTTCTTGGAGAGCAAAGGTATTAATGAGGGAGAGGACAGTGAATCCCCTGTTGTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

227

Amino Acids

25.26

Weight (kDa)

9.39

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 72 - 190 1e-43 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 174
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 1 cut(s) 459
AfaI GTAC 2 cut(s) 248, 275
AfeI AGCGCT 1 cut(s) 109
AflII CTTAAG 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 235
AgsI TTSAA 3 cut(s) 227, 259, 608
AjnI CCWGG 2 cut(s) 199, 295
AluBI AGCT 4 cut(s) 192, 368, 375, 416
AluI AGCT 4 cut(s) 192, 368, 375, 416
Alw26I GTCTC 1 cut(s) 515
AlwI GGATC 1 cut(s) 88
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 2 cut(s) 141, 299
ApeKI GCWGC 5 cut(s) 69, 100, 365, 368, 413
ApoI RAATTY 1 cut(s) 459
AseI ATTAAT 1 cut(s) 644
AsiGI ACCGGT 1 cut(s) 235
AspLEI GCGC 2 cut(s) 110, 358
AspS9I GGNCC 3 cut(s) 293, 299, 391
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 250, 562
AvaII GGWCC 2 cut(s) 293, 391
AxyI CCTNAGG 1 cut(s) 561
BauI CACGAG 1 cut(s) 277
BbvI GCAGC 5 cut(s) 81, 112, 355, 377, 425
BccI CCATC 5 cut(s) 146, 391, 465, 522, 530
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 2 cut(s) 201, 297
BcnI CCSGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 515
BfaI CTAG 2 cut(s) 36, 410
BfoI RGCGCY 2 cut(s) 111, 359
BfrI CTTAAG 1 cut(s) 454
BisI GCNGC 5 cut(s) 70, 101, 366, 369, 414
BlsI GCNGC 5 cut(s) 71, 102, 367, 370, 415
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 3 cut(s) 8, 201, 297
Bme18I GGWCC 2 cut(s) 293, 391
BmgT120I GGNCC 3 cut(s) 293, 299, 391
BmiI GGNNCC 7 cut(s) 198, 219, 268, 294, 392, 393, 421
BmrFI CCNGG 3 cut(s) 8, 201, 297
BpuMI CCSGG 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 123, 200, 296
BsaWI WCCGGW 2 cut(s) 235, 422
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 529
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 201, 297
BseDI CCNNGG 3 cut(s) 123, 200, 296
BseGI GGATG 2 cut(s) 476, 533
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 529
BseXI GCAGC 5 cut(s) 81, 112, 355, 377, 425
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 2 cut(s) 143, 301
BshTI ACCGGT 1 cut(s) 235
BsiSI CCGG 3 cut(s) 7, 236, 423
BslFI GGGAC 4 cut(s) 24, 169, 306, 404
BsmAI GTCTC 1 cut(s) 515
BsmFI GGGAC 4 cut(s) 24, 169, 306, 404
BsnI GGCC 2 cut(s) 143, 301
Bsp143I GATC 3 cut(s) 93, 286, 447
BspACI CCGC 1 cut(s) 174
BspANI GGCC 2 cut(s) 143, 301
BspCNI CTCAG 1 cut(s) 553
BspLI GGNNCC 7 cut(s) 198, 219, 268, 294, 392, 393, 421
BspPI GGATC 1 cut(s) 88
BspTI CTTAAG 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 1 cut(s) 529
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 123, 200, 296
BssMI GATC 3 cut(s) 93, 286, 447
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 201, 297
Bst4CI ACNGT 3 cut(s) 346, 604, 661
Bst6I CTCTTC 1 cut(s) 513
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 476, 533
BstH2I RGCGCY 2 cut(s) 111, 359
BstHHI GCGC 2 cut(s) 110, 358
BstKTI GATC 3 cut(s) 96, 289, 450
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 3 cut(s) 93, 286, 447
BstMWI GCNNNNNNNGC 2 cut(s) 97, 362
BstNI CCWGG 2 cut(s) 201, 297
BstSCI CCNGG 3 cut(s) 6, 199, 295
BstV1I GCAGC 5 cut(s) 81, 112, 355, 377, 425
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 2 cut(s) 143, 301
BtsCI GGATG 2 cut(s) 476, 533
BtsIMutI CAGTG 2 cut(s) 600, 666
Cac8I GCNNGC 1 cut(s) 373
CfoI GCGC 2 cut(s) 110, 358
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 3 cut(s) 293, 299, 391
Csp6I GTAC 2 cut(s) 247, 274
CspAI ACCGGT 1 cut(s) 235
CviAII CATG 1 cut(s) 87
CviJI RGCY 7 cut(s) 143, 192, 199, 301, 368, 375, 416
CviKI_1 RGCY 7 cut(s) 143, 192, 199, 301, 368, 375, 416
CviQI GTAC 2 cut(s) 247, 274
DdeI CTNAG 1 cut(s) 561
DpnI GATC 3 cut(s) 95, 288, 449
DpnII GATC 3 cut(s) 93, 286, 447
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 293, 391
Eco47III AGCGCT 1 cut(s) 109
Eco81I CCTNAGG 1 cut(s) 561
EcoO109I RGGNCCY 2 cut(s) 299, 391
EcoRII CCWGG 2 cut(s) 199, 295
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 1 cut(s) 90
FaiI YATR 5 cut(s) 88, 362, 509, 534, 581
FaqI GGGAC 4 cut(s) 24, 169, 306, 404
FatI CATG 1 cut(s) 86
Fnu4HI GCNGC 5 cut(s) 70, 101, 366, 369, 414
FokI GGATG 2 cut(s) 483, 540
Fsp4HI GCNGC 5 cut(s) 70, 101, 366, 369, 414
FspBI CTAG 2 cut(s) 36, 410
GlaI GCGC 2 cut(s) 109, 357
GluI GCNGC 5 cut(s) 70, 101, 366, 369, 414
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 2 cut(s) 111, 359
HaeIII GGCC 2 cut(s) 143, 301
HapII CCGG 3 cut(s) 7, 236, 423
HhaI GCGC 2 cut(s) 110, 358
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 2 cut(s) 108, 356
HinP1I GCGC 2 cut(s) 108, 356
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 1 cut(s) 664
HpaII CCGG 3 cut(s) 7, 236, 423
HphI GGTGA 2 cut(s) 250, 562
Hpy166II GTNNAC 1 cut(s) 600
Hpy188I TCNGA 2 cut(s) 189, 208
Hpy8I GTNNAC 1 cut(s) 600
HpyAV CCTTC 3 cut(s) 253, 257, 320
HpyCH4III ACNGT 3 cut(s) 346, 604, 661
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 1 cut(s) 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 362
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 2 cut(s) 108, 356
KflI GGGWCCC 1 cut(s) 391
Kzo9I GATC 3 cut(s) 93, 286, 447
LmnI GCTCC 3 cut(s) 9, 196, 353
Lsp1109I GCAGC 5 cut(s) 81, 112, 355, 377, 425
MaeI CTAG 2 cut(s) 36, 410
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 2 cut(s) 334, 476
MalI GATC 3 cut(s) 95, 288, 449
MboI GATC 3 cut(s) 93, 286, 447
MboII GAAGA 2 cut(s) 530, 615
MflI RGATCY 1 cut(s) 93
MluCI AATT 3 cut(s) 210, 459, 608
MnlI CCTC 7 cut(s) 155, 208, 314, 405, 556, 642, 648
MseI TTAA 3 cut(s) 378, 455, 644
MspA1I CMGCKG 2 cut(s) 368, 416
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 3 cut(s) 7, 236, 423
MspR9I CCNGG 3 cut(s) 8, 201, 297
MvaI CCWGG 2 cut(s) 201, 297
MwoI GCNNNNNNNGC 2 cut(s) 97, 362
NciI CCSGG 1 cut(s) 8
NdeII GATC 3 cut(s) 93, 286, 447
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 7 cut(s) 198, 219, 268, 294, 392, 393, 421
PfeI GAWTC 1 cut(s) 664
PinAI ACCGGT 1 cut(s) 235
PkrI GCNGC 5 cut(s) 71, 102, 367, 370, 415
PpuMI RGGWCCY 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 199, 295
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 199, 295
PspN4I GGNNCC 7 cut(s) 198, 219, 268, 294, 392, 393, 421
PspPI GGNCC 3 cut(s) 293, 299, 391
PspPPI RGGWCCY 1 cut(s) 391
PsuI RGATCY 1 cut(s) 93
PvuII CAGCTG 2 cut(s) 368, 416
RsaI GTAC 2 cut(s) 248, 275
RsaNI GTAC 2 cut(s) 247, 274
SaqAI TTAA 3 cut(s) 378, 455, 644
SatI GCNGC 5 cut(s) 70, 101, 366, 369, 414
Sau3AI GATC 3 cut(s) 93, 286, 447
Sau96I GGNCC 3 cut(s) 293, 299, 391
ScaI AGTACT 1 cut(s) 248
ScrFI CCNGG 3 cut(s) 8, 201, 297
SinI GGWCC 2 cut(s) 293, 391
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 3 cut(s) 210, 459, 608
SsiI CCGC 1 cut(s) 174
SspMI CTAG 2 cut(s) 36, 410
StyD4I CCNGG 3 cut(s) 6, 199, 295
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 346, 604, 661
TaiI ACGT 1 cut(s) 483
TasI AATT 3 cut(s) 210, 459, 608
TatI WGTACW 2 cut(s) 246, 273
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 3 cut(s) 378, 455, 644
Tru9I TTAA 3 cut(s) 378, 455, 644
TscAI CASTG 2 cut(s) 607, 666
TseI GCWGC 5 cut(s) 69, 100, 365, 368, 413
TspDTI ATGAA 1 cut(s) 601
TspRI CASTG 2 cut(s) 607, 666
Vha464I CTTAAG 1 cut(s) 454
VpaK11BI GGWCC 2 cut(s) 293, 391
VspI ATTAAT 1 cut(s) 644
XapI RAATTY 1 cut(s) 459
XspI CTAG 2 cut(s) 36, 410
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.