Rorug02G0142400

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007147
Physical Location & Seq
Reverse (-)
12521582 .. 12526075
4494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0142400.1

Sequence Viewer

Length: 1707 bp
ATGTGGCGGAGGAGCTTTAGCAGCGGCGCCGGCGCAGCCGCAAGCGCGTTGAAGGACAAGAAATGGGACGCGCTGGTCATCGGAGGCGGCCACAACGGCCTCACTGCAGCCGCGTACCTCGCACGAGCCGGCCTCTCCGTCGCAGTCCTCGAACGCCGCCACGTCCTCGGCGGAGCCGCCGTGACGGAAGAGTTCATTCCAGGATTCAAGTTCTCCCGGTGTAGCTACCTCCAGAGCCTCCTCCGTCCCGCCGTTATCAAGGAATTAGAGCTAGCTCGGCATGGAATGAAGCTACTGAAGAGGAGTCCGTCGTCGTTTACGCCTTGTTTGGACGGACGTTATCTTCTTTTAGGGCCGGAAAAGGACCTTAACCATTCAGAGATTGCTAAGTTCTCCAAGCTAGACGCTGATGCTTATCCAAGATATGAGAATCAGCTAGAGAAGTTCTGTCAGTTTATGGATCCTTTGCTGGATTCAGCTCCTCCAGAATCTTCGCAAGGCGCCTCCTCGGCTACTTTTGGTGACCGGTTGAAGGACAAGATGCACAAGTCGATGTTTTGGGCTCAGCTTCTGAGACAGGCTGCTGTCTTAGGCCAGAAAGATATGGTAGACTTTATGGACCTTCTGTTATCCCCGGCTTCGAAGGTTTTGAACAACTGGTTTGAGACTGATGTTCTGAAGGCAACCCTTGCAACAGATGCTGTAATAGGAACTACGGGAAGTGTCCATACACCAGGGAGTGGATATGTTCTGCTACATCATGTGATGGGAGAAACTGACGGCGAGCGCGGAATTTGGTCGTATGTTGAAGGTGGGATGGGTTCAGTATCCATGGCTATTGGTAATGCTGCTAGGGAAGCTGGGGCTCATATTGTCACAAGTGCAGAGGTTCAGCAAATGTTAATTAATGACTCTGGCAAAGTGGATGGGGTTTTGCTATCTGATGGTTCACGGGTGCACTCTTCAATTGTTTTATCAAATGCAACCCCTTATAAAACATTCAAGGAGTTGGTGCCTGATGATGTTCTTCCAGATGATTTTCTATGTGCAATTAAATACTCAGATTACAGCTCTGCTACTACGAAGATAAATATAGCGGTTGATCAGTTGCCCCAGTTTAAGAGCTGCAAGTTAAGTCATCCTCATGCAGGTCCTCAGCATATGGGCACCATTCACATTGGTTCCGAGAGTATGGAAGAGATTCACTTGGCCTGTCAAGATGCTGTTAATGGATTACCATCACAAAGACCAGTTATCGAGATGACAATTCCATCTGTACTGGACAAGACTATATCTCCACCTGGTAAGCATGTGATCAACTTGTTTATTCAATACACACCGTATAGTCCATCAGATGGACACTGGGGAGATCCTGTTTACAGAGAATCATTTGCAAAAAAGTGTTTCACCTTAATTGATGAATATGCCCCTGGGTTCAGTTCATCAGTAATTGGTTACGACATGTTGACTCCACCAGACCTTGAAAGGGAAATTGGTTTGACAGGAGGGAATATCTTCCATGGTGCTATGGGATTGGATTCACTCTTCCTCATGCGACCTGTAAAAGGATGGTCAAATTATAGAACACCACTTCCGGGTCTGTATTTGTGCGGTAGCGGGGCCCACCCAGGGGGCGGTGTGATGGGGGCTCCCGGGCGTAATGCTGCTCGTGTGGCTTTGGAAGATGTAAACAAGCCATTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009605 GO:0009991 GO:0031667 GO:0042594 GO:0050896 GO:1990641
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

568

Amino Acids

61.43

Weight (kDa)

6.35

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 21 - 60 6.9e-06 Thi4 family
DAO PF01266 23 - 59 2.4e-07 FAD dependent oxidoreductase
FAD_oxidored PF12831 23 - 62 2.2e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 26 - 73 4.1e-11 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 33 - 369 7.4e-11 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 1139
AccB1I GGYRCC 4 cut(s) 26, 500, 1012, 1166
AccB7I CCANNNNNTGG 2 cut(s) 740, 1355
AccI GTMKAC 1 cut(s) 609
AccII CGCG 4 cut(s) 47, 71, 113, 789
AclWI GGATC 3 cut(s) 455, 468, 1364
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 792
AcuI CTGAAG 2 cut(s) 317, 698
AcyI GRCGYC 2 cut(s) 27, 501
AfaI GTAC 2 cut(s) 116, 1278
AflIII ACRYGT 1 cut(s) 1461
AgeI ACCGGT 1 cut(s) 525
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 5 cut(s) 199, 733, 1300, 1429, 1627
AjuI GAANNNNNNNTTGG 2 cut(s) 1476, 1508
AloI GAACNNNNNNTCC 2 cut(s) 1576, 1608
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 2 cut(s) 568, 659
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 3 cut(s) 455, 468, 1364
AlwNI CAGNNNCTG 2 cut(s) 571, 701
Ama87I CYCGRG 1 cut(s) 1652
AoxI GGCC 7 cut(s) 88, 97, 130, 353, 592, 1209, 1620
ApaI GGGCCC 1 cut(s) 1624
ApaLI GTGCAC 1 cut(s) 956
ApeKI GCWGC 7 cut(s) 21, 35, 107, 581, 848, 1125, 1664
ApoI RAATTY 1 cut(s) 792
AseI ATTAAT 1 cut(s) 906
AsiGI ACCGGT 1 cut(s) 525
Asp700I GAANNNNTTC 2 cut(s) 1200, 1514
AspLEI GCGC 6 cut(s) 29, 35, 47, 73, 503, 789
AspS9I GGNCC 6 cut(s) 353, 364, 619, 1151, 1620, 1621
AsuC2I CCSGG 5 cut(s) 217, 635, 1596, 1653, 1654
AsuHPI GGTGA 2 cut(s) 533, 1399
AsuII TTCGAA 1 cut(s) 641
AsuNHI GCTAGC 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 1652
AvaII GGWCC 3 cut(s) 364, 619, 1151
BaeGI GKGCMC 3 cut(s) 960, 1169, 1624
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 4 cut(s) 26, 500, 1012, 1166
BanII GRGCYC 4 cut(s) 565, 868, 1624, 1651
BarI GAAGNNNNNNTAC 2 cut(s) 712, 744
BauI CACGAG 2 cut(s) 123, 1668
Bbv12I GWGCWC 1 cut(s) 960
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 7 cut(s) 33, 47, 119, 568, 835, 1112, 1651
BceAI ACGGC 4 cut(s) 112, 164, 236, 796
BciT130I CCWGG 5 cut(s) 201, 735, 1302, 1431, 1629
BciVI GTATCC 1 cut(s) 838
BclI TGATCA 2 cut(s) 1102, 1314
BcnI CCSGG 5 cut(s) 217, 635, 1596, 1653, 1654
BcoDI GTCTC 2 cut(s) 568, 659
BfaI CTAG 4 cut(s) 272, 401, 437, 852
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 2 cut(s) 30, 504
BfuAI ACCTGC 1 cut(s) 1139
BfuI GTATCC 1 cut(s) 838
BglI GCCNNNNNGGC 2 cut(s) 96, 509
BlpI GCTNAGC 1 cut(s) 564
Bme18I GGWCC 3 cut(s) 364, 619, 1151
BmeT110I CYCGRG 1 cut(s) 1652
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 6 cut(s) 353, 364, 619, 1151, 1620, 1621
BmrI ACTGGG 2 cut(s) 1108, 1372
BmsI GCATC 4 cut(s) 400, 531, 688, 1210
BmtI GCTAGC 1 cut(s) 275
BmuI ACTGGG 2 cut(s) 1108, 1372
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpmI CTGGAG 2 cut(s) 215, 468
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 641
BpuMI CCSGG 5 cut(s) 217, 635, 1596, 1653, 1654
BsaBI GATNNNNATC 2 cut(s) 414, 1237
BsaHI GRCGYC 2 cut(s) 27, 501
BsaWI WCCGGW 1 cut(s) 525
BsaXI ACNNNNNCTCC 6 cut(s) 165, 195, 1279, 1309, 1359, 1389
Bse118I RCCGGY 3 cut(s) 29, 128, 525
Bse1I ACTGG 5 cut(s) 662, 1114, 1250, 1284, 1367
Bse8I GATNNNNATC 2 cut(s) 414, 1237
BseBI CCWGG 5 cut(s) 201, 735, 1302, 1431, 1629
BseGI GGATG 4 cut(s) 822, 931, 1138, 1574
BseJI GATNNNNATC 2 cut(s) 414, 1237
BseMII CTCAG 4 cut(s) 563, 578, 1074, 1169
BseNI ACTGG 5 cut(s) 662, 1114, 1250, 1284, 1367
BseRI GAGGAG 5 cut(s) 25, 230, 316, 471, 496
BseSI GKGCMC 3 cut(s) 960, 1169, 1624
BseXI GCAGC 7 cut(s) 33, 47, 119, 568, 835, 1112, 1651
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 1 cut(s) 903
Bsh1236I CGCG 4 cut(s) 47, 71, 113, 789
BshFI GGCC 7 cut(s) 90, 99, 132, 355, 594, 1211, 1622
BshNI GGYRCC 4 cut(s) 26, 500, 1012, 1166
BshTI ACCGGT 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 960
BsiHKCI CYCGRG 1 cut(s) 1652
BsiSI CCGG 8 cut(s) 30, 129, 217, 356, 526, 635, 1595, 1653
BslFI GGGAC 2 cut(s) 80, 231
BsmAI GTCTC 2 cut(s) 568, 659
BsmFI GGGAC 2 cut(s) 80, 231
BsnI GGCC 7 cut(s) 90, 99, 132, 355, 594, 1211, 1622
BsoBI CYCGRG 1 cut(s) 1652
Bsp119I TTCGAA 1 cut(s) 641
Bsp120I GGGCCC 1 cut(s) 1620
Bsp1286I GDGCHC 6 cut(s) 565, 868, 960, 1169, 1624, 1651
Bsp143I GATC 4 cut(s) 460, 1102, 1314, 1369
Bsp1720I GCTNAGC 1 cut(s) 564
Bsp19I CCATGG 2 cut(s) 831, 1519
BspANI GGCC 7 cut(s) 90, 99, 132, 355, 594, 1211, 1622
BspCNI CTCAG 4 cut(s) 564, 577, 1073, 1168
BspFNI CGCG 4 cut(s) 47, 71, 113, 789
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 1139
BspOI GCTAGC 1 cut(s) 275
BspPI GGATC 3 cut(s) 455, 468, 1364
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 4 cut(s) 26, 500, 1012, 1166
BsrFI RCCGGY 3 cut(s) 29, 128, 525
BsrI ACTGG 5 cut(s) 662, 1114, 1250, 1284, 1367
BssAI RCCGGY 3 cut(s) 29, 128, 525
BssMI GATC 4 cut(s) 460, 1102, 1314, 1369
BssNI GRCGYC 2 cut(s) 27, 501
BssSI CACGAG 2 cut(s) 123, 1668
BssT1I CCWWGG 2 cut(s) 831, 1519
Bst2BI CACGAG 2 cut(s) 123, 1668
Bst2UI CCWGG 5 cut(s) 201, 735, 1302, 1431, 1629
Bst4CI ACNGT 1 cut(s) 1341
Bst6I CTCTTC 5 cut(s) 183, 293, 967, 1191, 1550
BstACI GRCGYC 2 cut(s) 27, 501
BstAPI GCANNNNNTGC 2 cut(s) 689, 698
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 5 cut(s) 31, 43, 130, 273, 785
BstDEI CTNAG 6 cut(s) 387, 564, 572, 589, 1060, 1155
BstDSI CCRYGG 2 cut(s) 831, 1519
BstEII GGTNACC 1 cut(s) 521
BstENI CCTNNNNNAGG 2 cut(s) 1146, 1563
BstF5I GGATG 4 cut(s) 822, 931, 1138, 1574
BstFNI CGCG 4 cut(s) 47, 71, 113, 789
BstH2I RGCGCY 2 cut(s) 30, 504
BstHHI GCGC 6 cut(s) 29, 35, 47, 73, 503, 789
BstKTI GATC 4 cut(s) 463, 1105, 1317, 1372
BstMAI GTCTC 2 cut(s) 568, 659
BstMBI GATC 4 cut(s) 460, 1102, 1314, 1369
BstNI CCWGG 5 cut(s) 201, 735, 1302, 1431, 1629
BstNSI RCATGY 2 cut(s) 1313, 1465
BstPI GGTNACC 1 cut(s) 521
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 3 cut(s) 960, 1169, 1624
BstUI CGCG 4 cut(s) 47, 71, 113, 789
BstV1I GCAGC 7 cut(s) 33, 47, 119, 568, 835, 1112, 1651
BstX2I RGATCY 2 cut(s) 460, 1369
BstYI RGATCY 2 cut(s) 460, 1369
BsuI GTATCC 1 cut(s) 838
BsuRI GGCC 7 cut(s) 90, 99, 132, 355, 594, 1211, 1622
BtgI CCRYGG 2 cut(s) 831, 1519
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 4 cut(s) 822, 931, 1138, 1574
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 102, 1360
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 5 cut(s) 31, 43, 130, 273, 785
CaiI CAGNNNCTG 2 cut(s) 571, 701
CfoI GCGC 6 cut(s) 29, 35, 47, 73, 503, 789
Cfr10I RCCGGY 3 cut(s) 29, 128, 525
Cfr13I GGNCC 6 cut(s) 353, 364, 619, 1151, 1620, 1621
Cfr9I CCCGGG 1 cut(s) 1652
CseI GACGC 2 cut(s) 77, 413
CsiI ACCWGGT 1 cut(s) 1300
Csp6I GTAC 2 cut(s) 115, 1277
CspAI ACCGGT 1 cut(s) 525
CviAII CATG 8 cut(s) 281, 761, 832, 1145, 1310, 1462, 1520, 1552
CviQI GTAC 2 cut(s) 115, 1277
DdeI CTNAG 6 cut(s) 387, 564, 572, 589, 1060, 1155
DinI GGCGCC 2 cut(s) 28, 502
DpnI GATC 4 cut(s) 462, 1104, 1316, 1371
DpnII GATC 4 cut(s) 460, 1102, 1314, 1369
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 5 cut(s) 183, 293, 967, 1191, 1550
EarI CTCTTC 5 cut(s) 183, 293, 967, 1191, 1550
EciI GGCGGA 2 cut(s) 22, 186
Eco130I CCWWGG 2 cut(s) 831, 1519
Eco24I GRGCYC 4 cut(s) 565, 868, 1624, 1651
Eco47I GGWCC 3 cut(s) 364, 619, 1151
Eco57I CTGAAG 2 cut(s) 317, 698
Eco88I CYCGRG 1 cut(s) 1652
Eco91I GGTNACC 1 cut(s) 521
EcoNI CCTNNNNNAGG 2 cut(s) 1146, 1563
EcoO109I RGGNCCY 3 cut(s) 364, 1151, 1620
EcoO65I GGTNACC 1 cut(s) 521
EcoRII CCWGG 5 cut(s) 199, 733, 1300, 1429, 1627
EcoT14I CCWWGG 2 cut(s) 831, 1519
EcoT38I GRGCYC 4 cut(s) 565, 868, 1624, 1651
EgeI GGCGCC 2 cut(s) 28, 502
EheI GGCGCC 2 cut(s) 28, 502
ErhI CCWWGG 2 cut(s) 831, 1519
FaeI CATG 8 cut(s) 284, 764, 835, 1148, 1313, 1465, 1523, 1555
FaqI GGGAC 2 cut(s) 80, 231
FatI CATG 8 cut(s) 280, 760, 831, 1144, 1309, 1461, 1519, 1551
FauI CCCGC 2 cut(s) 256, 1610
FauNDI CATATG 1 cut(s) 1161
FbaI TGATCA 2 cut(s) 1102, 1314
FblI GTMKAC 1 cut(s) 609
FokI GGATG 4 cut(s) 829, 938, 1125, 1581
FriOI GRGCYC 4 cut(s) 565, 868, 1624, 1651
FspBI CTAG 4 cut(s) 272, 401, 437, 852
GlaI GCGC 6 cut(s) 28, 34, 46, 72, 502, 788
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 2 cut(s) 215, 468
HaeII RGCGCY 2 cut(s) 30, 504
HaeIII GGCC 7 cut(s) 90, 99, 132, 355, 594, 1211, 1622
HapII CCGG 8 cut(s) 30, 129, 217, 356, 526, 635, 1595, 1653
HgaI GACGC 2 cut(s) 77, 413
HhaI GCGC 6 cut(s) 29, 35, 47, 73, 503, 789
Hin1I GRCGYC 2 cut(s) 27, 501
Hin1II CATG 8 cut(s) 284, 764, 835, 1148, 1313, 1465, 1523, 1555
Hin6I GCGC 6 cut(s) 27, 33, 45, 71, 501, 787
HinP1I GCGC 6 cut(s) 27, 33, 45, 71, 501, 787
HincII GTYRAC 1 cut(s) 1467
HindII GTYRAC 1 cut(s) 1467
HpaII CCGG 8 cut(s) 30, 129, 217, 356, 526, 635, 1595, 1653
HphI GGTGA 2 cut(s) 533, 1399
Hpy166II GTNNAC 7 cut(s) 318, 610, 950, 958, 1378, 1467, 1690
Hpy188I TCNGA 8 cut(s) 83, 379, 573, 678, 943, 1063, 1186, 1354
Hpy188III TCNNGA 5 cut(s) 232, 485, 1031, 1217, 1258
Hpy8I GTNNAC 7 cut(s) 318, 610, 950, 958, 1378, 1467, 1690
Hpy99I CGWCG 3 cut(s) 143, 313, 316
HpyAV CCTTC 6 cut(s) 46, 526, 632, 637, 673, 803
HpyCH4III ACNGT 1 cut(s) 1341
HpyCH4IV ACGT 2 cut(s) 162, 337
HpyF3I CTNAG 6 cut(s) 387, 564, 572, 589, 1060, 1155
HpySE526I ACGT 2 cut(s) 162, 337
Hsp92I GRCGYC 2 cut(s) 27, 501
Hsp92II CATG 8 cut(s) 284, 764, 835, 1148, 1313, 1465, 1523, 1555
HspAI GCGC 6 cut(s) 27, 33, 45, 71, 501, 787
KasI GGCGCC 2 cut(s) 26, 500
KroI GCCGGC 2 cut(s) 29, 128
KroNI GCCGGC 2 cut(s) 31, 130
Ksp22I TGATCA 2 cut(s) 1102, 1314
Kzo9I GATC 4 cut(s) 460, 1102, 1314, 1369
LmnI GCTCC 4 cut(s) 12, 173, 484, 1654
Lsp1109I GCAGC 7 cut(s) 33, 47, 119, 568, 835, 1112, 1651
LweI GCATC 4 cut(s) 400, 531, 688, 1210
MabI ACCWGGT 1 cut(s) 1300
MaeI CTAG 4 cut(s) 272, 401, 437, 852
MaeII ACGT 2 cut(s) 162, 337
MaeIII GTNAC 4 cut(s) 181, 521, 874, 1454
MalI GATC 4 cut(s) 462, 1104, 1316, 1371
MboI GATC 4 cut(s) 460, 1102, 1314, 1369
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 2 cut(s) 460, 1369
MhlI GDGCHC 6 cut(s) 565, 868, 960, 1169, 1624, 1651
Mly113I GGCGCC 2 cut(s) 27, 501
MlyI GAGTC 3 cut(s) 313, 905, 1462
MreI CGCCGGCG 1 cut(s) 29
MroNI GCCGGC 2 cut(s) 29, 128
MroXI GAANNNNTTC 2 cut(s) 1200, 1514
MseI TTAA 8 cut(s) 369, 902, 906, 1053, 1119, 1133, 1227, 1412
MslI CAYNNNNRTG 2 cut(s) 1143, 1702
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 8 cut(s) 30, 129, 217, 356, 526, 635, 1595, 1653
MunI CAATTG 1 cut(s) 966
MvaI CCWGG 5 cut(s) 201, 735, 1302, 1431, 1629
MvnI CGCG 4 cut(s) 47, 71, 113, 789
NaeI GCCGGC 2 cut(s) 31, 130
NarI GGCGCC 2 cut(s) 27, 501
NciI CCSGG 5 cut(s) 217, 635, 1596, 1653, 1654
NcoI CCATGG 2 cut(s) 831, 1519
NdeI CATATG 1 cut(s) 1161
NdeII GATC 4 cut(s) 460, 1102, 1314, 1369
NgoMIV GCCGGC 2 cut(s) 29, 128
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 8 cut(s) 284, 764, 835, 1148, 1313, 1465, 1523, 1555
NmeAIII GCCGAG 3 cut(s) 147, 256, 488
NmuCI GTSAC 3 cut(s) 181, 521, 874
NspI RCATGY 2 cut(s) 1313, 1465
NspV TTCGAA 1 cut(s) 641
PacI TTAATTAA 1 cut(s) 906
PasI CCCWGGG 2 cut(s) 1430, 1628
PciI ACATGT 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 2 cut(s) 31, 130
PdmI GAANNNNTTC 2 cut(s) 1200, 1514
PfeI GAWTC 7 cut(s) 204, 430, 473, 488, 1201, 1385, 1538
PflMI CCANNNNNTGG 2 cut(s) 740, 1355
PfoI TCCNGGA 1 cut(s) 199
PinAI ACCGGT 1 cut(s) 525
PleI GAGTC 3 cut(s) 312, 905, 1462
PluTI GGCGCC 2 cut(s) 30, 504
PpsI GAGTC 3 cut(s) 312, 905, 1462
PpuMI RGGWCCY 2 cut(s) 364, 1151
PscI ACATGT 1 cut(s) 1461
PshBI ATTAAT 1 cut(s) 906
PsiI TTATAA 1 cut(s) 993
Psp5II RGGWCCY 2 cut(s) 364, 1151
Psp6I CCWGG 5 cut(s) 199, 733, 1300, 1429, 1627
PspEI GGTNACC 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 5 cut(s) 199, 733, 1300, 1429, 1627
PspOMI GGGCCC 1 cut(s) 1620
PspPI GGNCC 6 cut(s) 353, 364, 619, 1151, 1620, 1621
PspPPI RGGWCCY 2 cut(s) 364, 1151
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 2 cut(s) 571, 701
PsuI RGATCY 2 cut(s) 460, 1369
RsaI GTAC 2 cut(s) 116, 1278
RsaNI GTAC 2 cut(s) 115, 1277
RseI CAYNNNNRTG 2 cut(s) 1143, 1702
SaqAI TTAA 8 cut(s) 369, 902, 906, 1053, 1119, 1133, 1227, 1412
Sau3AI GATC 4 cut(s) 460, 1102, 1314, 1369
Sau96I GGNCC 6 cut(s) 353, 364, 619, 1151, 1620, 1621
SchI GAGTC 3 cut(s) 313, 905, 1462
SduI GDGCHC 6 cut(s) 565, 868, 960, 1169, 1624, 1651
SexAI ACCWGGT 1 cut(s) 1300
SfaNI GCATC 4 cut(s) 400, 531, 688, 1210
SfcI CTRYAG 1 cut(s) 105
SfiI GGCCNNNNNGGCC 1 cut(s) 96
SfoI GGCGCC 2 cut(s) 28, 502
SfuI TTCGAA 1 cut(s) 641
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 3 cut(s) 364, 619, 1151
SmaI CCCGGG 1 cut(s) 1654
SmiMI CAYNNNNRTG 2 cut(s) 1143, 1702
SspDI GGCGCC 2 cut(s) 26, 500
SspMI CTAG 4 cut(s) 272, 401, 437, 852
StyI CCWWGG 2 cut(s) 831, 1519
TaaI ACNGT 1 cut(s) 1341
TaiI ACGT 2 cut(s) 165, 340
TaqI TCGA 4 cut(s) 150, 551, 641, 1257
TatI WGTACW 1 cut(s) 1276
TauI GCSGC 6 cut(s) 27, 41, 90, 113, 159, 179
TfiI GAWTC 7 cut(s) 204, 430, 473, 488, 1201, 1385, 1538
Tru1I TTAA 8 cut(s) 369, 902, 906, 1053, 1119, 1133, 1227, 1412
Tru9I TTAA 8 cut(s) 369, 902, 906, 1053, 1119, 1133, 1227, 1412
TscAI CASTG 2 cut(s) 109, 1367
TseFI GTSAC 3 cut(s) 181, 521, 874
TseI GCWGC 7 cut(s) 21, 35, 107, 581, 848, 1125, 1664
Tsp45I GTSAC 3 cut(s) 181, 521, 874
TspDTI ATGAA 4 cut(s) 184, 302, 1431, 1434
TspGWI ACGGA 5 cut(s) 127, 200, 233, 297, 348
TspMI CCCGGG 1 cut(s) 1652
TspRI CASTG 2 cut(s) 109, 1367
Van91I CCANNNNNTGG 2 cut(s) 740, 1355
VneI GTGCAC 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 364, 619, 1151
VspI ATTAAT 1 cut(s) 906
XagI CCTNNNNNAGG 2 cut(s) 1146, 1563
XapI RAATTY 1 cut(s) 792
XceI RCATGY 2 cut(s) 1313, 1465
XmaI CCCGGG 1 cut(s) 1652
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 2 cut(s) 1200, 1514
XspI CTAG 4 cut(s) 272, 401, 437, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.