AT5G67500

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003645
Physical Location & Seq
Forward (+)
26935075 .. 26937650
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67500.1

Sequence Viewer

Length: 918 bp
ATGAGCAAAGGTCCAGGACTCTTCACCGATATCGGCAAGAAAGCCAAAGATCTGTTGACGAGAGACTACAATTCCGATCAGAAGTTCAGTATCTCCACTTACAGTGCCTCCGGCGTGGCCCTTACGTCTACTGCTCTGAAGAAAGGAGGAGTTCATGCTGCTGATGTTGCCACCCAATACAAGTACAAGAATGCTTTGTTTGATGTTAAAATCGACACTGATTCTAGTGTTTTGACAACAGTCACACTTACCGAGATCCTCCCATCAACAAAAGCCATTGCCTCCTTCAAAGTCCCTGATTATAATTCCGCCAAGCTCGAGGTGCAATACTTCCACGACCACGCAACAGTTACCGCTGCTGCAGCTTTGAAACAAAACCCACTAATTGACATAACAGCTACTCTTGGTTCGCCAGTCATCTCATTTGGTGCTGAAGCTGGATATGACACAACCTCCAAAACTTTCACCAAGTACAATGCCGGGATCAGTGTGACAAAACCAGATGCTTGCCTCTCCATAATATTGGGAGACAAAGGAGACTCACTCAAAGCATCATACCTTCATCACTTTGATGAATTTAAGAGAACCGCAGCAGTTGGTGAGGTTTACAGGAAGTTCTCGACAAATGAAAACACGATAACGGTTGGTGGATTGTATGCGATTGATCATTCGACTGCAGTGAAAGCTAAGCTCAACAACCATGGCACACTTGGTGCTCTTCTGCAGCACGAGGTCCTGCCCAGATCACTAGTGACTGTTTCCAGTGAGATTGACACTAAGGCTTTAGAAAAGCATCCAAGGAACAATGATAGGAGGATACAAATAAAAACCGTTTCTACAACGAGCCAGTTTGGTTTAGTTGATCAGCTGCTTGTCATCGAGGTACATTCGCAAAGGAGATTCTCCCAATATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

305

Amino Acids

33.13

Weight (kDa)

8.95

Isoelectric Point (pI)

24.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 4 - 263 1.5e-71 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccI GTMKAC 1 cut(s) 128
AciI CCGC 3 cut(s) 309, 354, 588
AclWI GGATC 2 cut(s) 250, 491
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 2 cut(s) 158, 453
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 3 cut(s) 185, 473, 885
AgsI TTSAA 2 cut(s) 289, 370
AhlI ACTAGT 1 cut(s) 748
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 7 cut(s) 316, 365, 398, 437, 686, 691, 868
AluI AGCT 7 cut(s) 316, 365, 398, 437, 686, 691, 868
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 3 cut(s) 57, 522, 531
AlwI GGATC 2 cut(s) 250, 491
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 7 cut(s) 158, 356, 359, 362, 590, 724, 868
ApoI RAATTY 1 cut(s) 575
AspS9I GGNCC 3 cut(s) 11, 118, 733
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 16, 457, 611
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 11, 733
BauI CACGAG 1 cut(s) 728
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 7 cut(s) 145, 343, 346, 374, 602, 736, 855
BccI CCATC 1 cut(s) 271
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 810
BclI TGATCA 2 cut(s) 664, 862
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 3 cut(s) 57, 522, 531
BcuI ACTAGT 1 cut(s) 748
BfaI CTAG 2 cut(s) 225, 749
BfmI CTRYAG 3 cut(s) 360, 675, 722
BfuI GTATCC 1 cut(s) 810
BglII AGATCT 1 cut(s) 49
BisI GCNGC 7 cut(s) 159, 357, 360, 363, 591, 725, 869
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 7 cut(s) 160, 358, 361, 364, 592, 726, 870
Bme1390I CCNGG 2 cut(s) 15, 481
Bme18I GGWCC 2 cut(s) 11, 733
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 11, 118, 733
BmrFI CCNGG 2 cut(s) 15, 481
BmsI GCATC 3 cut(s) 493, 560, 802
BoxI GACNNNNGTC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 528, 560
Bpu1102I GCTNAGC 1 cut(s) 687
BpuMI CCSGG 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 700, 797
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 437, 467
Bse1I ACTGG 3 cut(s) 413, 762, 847
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 700, 797
BseGI GGATG 1 cut(s) 793
BseMI GCAATG 1 cut(s) 276
BseNI ACTGG 3 cut(s) 413, 762, 847
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 7 cut(s) 145, 343, 346, 374, 602, 736, 855
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 718
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 111, 480
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 3 cut(s) 57, 522, 531
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
Bsp1720I GCTNAGC 1 cut(s) 687
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 3 cut(s) 309, 354, 588
BspANI GGCC 1 cut(s) 119
BspMAI CTGCAG 3 cut(s) 364, 679, 726
BspPI GGATC 2 cut(s) 250, 491
BspQI GCTCTTC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 3 cut(s) 413, 762, 847
BssECI CCNNGG 2 cut(s) 700, 797
BssMI GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 2 cut(s) 700, 797
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 6 cut(s) 104, 241, 349, 643, 757, 832
Bst6I CTCTTC 2 cut(s) 26, 723
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 2 cut(s) 687, 777
BstDSI CCRYGG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 793
BstKTI GATC 7 cut(s) 52, 79, 258, 486, 667, 746, 865
BstMAI GTCTC 3 cut(s) 57, 522, 531
BstMBI GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
BstMWI GCNNNNNNNGC 4 cut(s) 167, 322, 362, 683
BstNI CCWGG 1 cut(s) 15
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 13, 479
BstSFI CTRYAG 3 cut(s) 360, 675, 722
BstV1I GCAGC 7 cut(s) 145, 343, 346, 374, 602, 736, 855
BstX2I RGATCY 2 cut(s) 49, 255
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 2 cut(s) 49, 255
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 1 cut(s) 793
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 5 cut(s) 109, 216, 493, 684, 769
Cac8I GCNNGC 1 cut(s) 508
Cfr13I GGNCC 3 cut(s) 11, 118, 733
Csp6I GTAC 3 cut(s) 184, 472, 884
CviAII CATG 2 cut(s) 155, 701
CviQI GTAC 3 cut(s) 184, 472, 884
DdeI CTNAG 2 cut(s) 687, 777
DpnI GATC 7 cut(s) 51, 78, 257, 485, 666, 745, 864
DpnII GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
DraIII CACNNNGTG 1 cut(s) 713
Eam1104I CTCTTC 2 cut(s) 26, 723
EarI CTCTTC 2 cut(s) 26, 723
EciI GGCGGA 1 cut(s) 298
Eco130I CCWWGG 2 cut(s) 700, 797
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 11, 733
Eco57I CTGAAG 2 cut(s) 158, 453
Eco88I CYCGRG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 2 cut(s) 700, 797
ErhI CCWWGG 2 cut(s) 700, 797
FaeI CATG 2 cut(s) 158, 704
FaiI YATR 8 cut(s) 156, 303, 392, 444, 518, 556, 657, 702
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 154, 700
FbaI TGATCA 2 cut(s) 664, 862
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 7 cut(s) 159, 357, 360, 363, 591, 725, 869
FokI GGATG 1 cut(s) 780
Fsp4HI GCNGC 7 cut(s) 159, 357, 360, 363, 591, 725, 869
FspBI CTAG 2 cut(s) 225, 749
GluI GCNGC 7 cut(s) 159, 357, 360, 363, 591, 725, 869
HaeIII GGCC 1 cut(s) 119
HapII CCGG 2 cut(s) 111, 480
Hin1II CATG 2 cut(s) 158, 704
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 4 cut(s) 18, 221, 539, 900
HpaII CCGG 2 cut(s) 111, 480
HphI GGTGA 3 cut(s) 16, 457, 611
Hpy166II GTNNAC 3 cut(s) 57, 129, 607
Hpy188I TCNGA 3 cut(s) 76, 81, 138
Hpy188III TCNNGA 1 cut(s) 619
Hpy8I GTNNAC 3 cut(s) 57, 129, 607
HpyAV CCTTC 2 cut(s) 295, 569
HpyCH4III ACNGT 6 cut(s) 104, 241, 349, 643, 757, 832
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 4 cut(s) 325, 362, 677, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 322, 362, 683
HpyF3I CTNAG 2 cut(s) 687, 777
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 2 cut(s) 158, 704
Ksp22I TGATCA 2 cut(s) 664, 862
Kzo9I GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
LguI GCTCTTC 1 cut(s) 723
Lsp1109I GCAGC 7 cut(s) 145, 343, 346, 374, 602, 736, 855
LweI GCATC 3 cut(s) 493, 560, 802
MaeI CTAG 2 cut(s) 225, 749
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 4 cut(s) 241, 349, 490, 751
MalI GATC 7 cut(s) 51, 78, 257, 485, 666, 745, 864
MboI GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
MboII GAAGA 3 cut(s) 13, 151, 710
MflI RGATCY 2 cut(s) 49, 255
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 4 cut(s) 70, 304, 384, 575
MlyI GAGTC 2 cut(s) 12, 533
MseI TTAA 2 cut(s) 207, 579
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 2 cut(s) 356, 868
MspI CCGG 2 cut(s) 111, 480
MspR9I CCNGG 2 cut(s) 15, 481
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 4 cut(s) 167, 322, 362, 683
NciI CCSGG 1 cut(s) 481
NcoI CCATGG 1 cut(s) 700
NdeII GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
NlaIII CATG 2 cut(s) 158, 704
NmuCI GTSAC 3 cut(s) 241, 490, 751
PaeR7I CTCGAG 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 221, 900
PfoI TCCNGGA 1 cut(s) 13
PkrI GCNGC 7 cut(s) 160, 358, 361, 364, 592, 726, 870
PleI GAGTC 2 cut(s) 12, 533
PpsI GAGTC 2 cut(s) 12, 533
PpuMI RGGWCCY 1 cut(s) 733
PshAI GACNNNNGTC 1 cut(s) 239
PsiI TTATAA 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspPI GGNCC 3 cut(s) 11, 118, 733
PspPPI RGGWCCY 1 cut(s) 733
PspXI VCTCGAGB 1 cut(s) 317
PstI CTGCAG 3 cut(s) 364, 679, 726
PsuI RGATCY 2 cut(s) 49, 255
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 3 cut(s) 185, 473, 885
RsaNI GTAC 3 cut(s) 184, 472, 884
RseI CAYNNNNRTG 1 cut(s) 570
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 207, 579
SatI GCNGC 7 cut(s) 159, 357, 360, 363, 591, 725, 869
Sau3AI GATC 7 cut(s) 49, 76, 255, 483, 664, 743, 862
Sau96I GGNCC 3 cut(s) 11, 118, 733
SchI GAGTC 2 cut(s) 12, 533
ScrFI CCNGG 2 cut(s) 15, 481
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 3 cut(s) 493, 560, 802
SfcI CTRYAG 3 cut(s) 360, 675, 722
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 11, 733
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SpeI ACTAGT 1 cut(s) 748
Sse9I AATT 4 cut(s) 70, 304, 384, 575
SsiI CCGC 3 cut(s) 309, 354, 588
SspI AATATT 2 cut(s) 522, 911
SspMI CTAG 2 cut(s) 225, 749
StyD4I CCNGG 2 cut(s) 13, 479
StyI CCWWGG 2 cut(s) 700, 797
TaaI ACNGT 6 cut(s) 104, 241, 349, 643, 757, 832
TaiI ACGT 1 cut(s) 128
TaqI TCGA 5 cut(s) 213, 318, 620, 671, 879
TasI AATT 4 cut(s) 70, 304, 384, 575
TatI WGTACW 2 cut(s) 183, 471
TfiI GAWTC 2 cut(s) 221, 900
Tru1I TTAA 2 cut(s) 207, 579
Tru9I TTAA 2 cut(s) 207, 579
TscAI CASTG 5 cut(s) 109, 223, 493, 684, 769
TseFI GTSAC 3 cut(s) 241, 490, 751
TseI GCWGC 7 cut(s) 158, 356, 359, 362, 590, 724, 868
Tsp45I GTSAC 3 cut(s) 241, 490, 751
TspDTI ATGAA 4 cut(s) 143, 551, 588, 642
TspRI CASTG 5 cut(s) 109, 223, 493, 684, 769
VpaK11BI GGWCC 2 cut(s) 11, 733
XapI RAATTY 1 cut(s) 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 128
XspI CTAG 2 cut(s) 225, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.