MD15G1296700.v1.1

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003645
Physical Location & Seq
Forward (+)
27844292 .. 27848045
3754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1296700.v1.1.491

Sequence Viewer

Length: 831 bp
ATGAGCAAGGGACCTGGACTTTTTACCGACATCGGCAAGAAAGCCAAAGATCTTCTGACCAAGGACTACAGCTCCGATCAAAAATTCACCATCTCCACCTACAGCGATGCCGGCGTGGCCCTCACCTCAACAGCAGTGAAGAAAGGAGGACTTTCCATTGGGGACATCTTGACACAATACAAGTACAAGAACACGGTGGTTGATGTCAAAGTTGACACAGAATCAAATATCTTGACGACCTTCACGATCAATGAGATTGTCCCATCCACAAAAACTATAGCTTCATTCAAAGCGCCTGATTTCAACTCTGGCAAGTTGGAGGTTCAGTACTTCCATGACCATGCAGCCGTCACTACAGCCGTTGCCTTGAATCAATCCCCAGCTATTGATTTTTCAGCCACCATTGGAACTCCAACCATTGCATTTGGTGCAGAAGGGGGTTATGACACTACGTCTGGAAACTTTACGAAGTATACTGCTGGAATTAGCATCACAAAGCCAGATCAGTCTGCTTCAATAATTCTGGGTGACAAGGGAGACAGTATAAGAGCATCATATGTGCATTATATGGATCTGTTGAAGAGTAGTGCTGCTGTGGGAGAGATCACTAGAAAGTTCTCCACGAACGAGAACACTTTCACAGTGGGGGGCCAATATGCTGTTGATATCTACACTGTAGTGAAAGCTAAGCTCAACAATCACGGAAAGCTAGGAGCCCTCCTGCAGCATGAAGTCATACCGAAGTCAGTGCTGACAATATCTGGTGAGGTAGACACCAAGGCCTTGGACAAAAATCCCAGGTTTGGCGTGGCCATTGCCCTCAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

277

Amino Acids

29.4

Weight (kDa)

8.47

Isoelectric Point (pI)

17.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 4 - 269 2.3e-74 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 473, 771
AclWI GGATC 1 cut(s) 579
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 2 cut(s) 185, 329
AfiI CCNNNNNNNGG 1 cut(s) 803
AgsI TTSAA 5 cut(s) 289, 304, 370, 516, 580
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 13, 797
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 6 cut(s) 72, 281, 383, 686, 691, 709
AluI AGCT 6 cut(s) 72, 281, 383, 686, 691, 709
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 1 cut(s) 579
AoxI GGCC 4 cut(s) 117, 649, 780, 810
ApeKI GCWGC 3 cut(s) 344, 590, 724
ApoI RAATTY 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 3 cut(s) 11, 118, 649
AsuHPI GGTGA 4 cut(s) 79, 115, 539, 776
AvaII GGWCC 1 cut(s) 11
BalI TGGCCA 1 cut(s) 812
BanII GRGCYC 1 cut(s) 718
BbvI GCAGC 3 cut(s) 356, 577, 736
BccI CCATC 2 cut(s) 98, 271
BceAI ACGGC 2 cut(s) 332, 344
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 2 cut(s) 15, 799
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 2 cut(s) 609, 710
BfmI CTRYAG 6 cut(s) 67, 100, 276, 354, 675, 722
BfoI RGCGCY 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 49
BisI GCNGC 3 cut(s) 345, 591, 725
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 3 cut(s) 346, 592, 726
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 2 cut(s) 15, 799
Bme18I GGWCC 1 cut(s) 11
BmeRI GACNNNNNGTC 1 cut(s) 451
BmgT120I GGNCC 3 cut(s) 11, 118, 649
BmiI GGNNCC 3 cut(s) 12, 650, 715
BmrFI CCNGG 2 cut(s) 15, 799
BmsI GCATC 3 cut(s) 97, 498, 560
Bpu1102I GCTNAGC 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 60, 777, 783, 797
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse118I RCCGGY 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 417, 813
BseBI CCWGG 2 cut(s) 15, 799
BseDI CCNNGG 4 cut(s) 60, 777, 783, 797
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMI GCAATG 2 cut(s) 417, 813
BseXI GCAGC 3 cut(s) 356, 577, 736
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 4 cut(s) 119, 651, 782, 812
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 3 cut(s) 24, 176, 245
BslI CCNNNNNNNGG 1 cut(s) 803
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 3 cut(s) 24, 176, 245
BsnI GGCC 4 cut(s) 119, 651, 782, 812
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 6 cut(s) 49, 76, 246, 502, 571, 603
Bsp1720I GCTNAGC 1 cut(s) 687
BspANI GGCC 4 cut(s) 119, 651, 782, 812
BspLI GGNNCC 3 cut(s) 12, 650, 715
BspMAI CTGCAG 1 cut(s) 726
BspPI GGATC 1 cut(s) 579
BsrDI GCAATG 2 cut(s) 417, 813
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 4 cut(s) 60, 777, 783, 797
BssMI GATC 6 cut(s) 49, 76, 246, 502, 571, 603
BssNAI GTATAC 1 cut(s) 474
BssT1I CCWWGG 3 cut(s) 60, 777, 783
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 15, 799
Bst4CI ACNGT 4 cut(s) 196, 542, 643, 676
Bst6I CTCTTC 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 687
BstF5I GGATG 1 cut(s) 263
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 6 cut(s) 52, 79, 249, 505, 574, 606
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 6 cut(s) 49, 76, 246, 502, 571, 603
BstMWI GCNNNNNNNGC 3 cut(s) 111, 116, 428
BstNI CCWGG 2 cut(s) 15, 799
BstSCI CCNGG 2 cut(s) 13, 797
BstSFI CTRYAG 6 cut(s) 67, 100, 276, 354, 675, 722
BstV1I GCAGC 3 cut(s) 356, 577, 736
BstX2I RGATCY 2 cut(s) 49, 571
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 2 cut(s) 49, 571
BstZ17I GTATAC 1 cut(s) 474
BsuRI GGCC 4 cut(s) 119, 651, 782, 812
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 263
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 648, 672, 753
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 3 cut(s) 11, 118, 649
Csp6I GTAC 2 cut(s) 184, 328
CviAII CATG 3 cut(s) 335, 341, 728
CviQI GTAC 2 cut(s) 184, 328
DdeI CTNAG 1 cut(s) 687
DpnI GATC 6 cut(s) 51, 78, 248, 504, 573, 605
DpnII GATC 6 cut(s) 49, 76, 246, 502, 571, 603
DriI GACNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 575
Eam1105I GACNNNNNGTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 60, 777, 783
Eco147I AGGCCT 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 718
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 13, 797
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 3 cut(s) 60, 777, 783
EcoT38I GRGCYC 1 cut(s) 718
ErhI CCWWGG 3 cut(s) 60, 777, 783
FaeI CATG 3 cut(s) 338, 344, 731
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 3 cut(s) 24, 176, 245
FatI CATG 3 cut(s) 334, 340, 727
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 2 cut(s) 473, 771
Fnu4HI GCNGC 3 cut(s) 345, 591, 725
FokI GGATG 1 cut(s) 250
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 3 cut(s) 345, 591, 725
FspBI CTAG 2 cut(s) 609, 710
GlaI GCGC 1 cut(s) 294
GluI GCNGC 3 cut(s) 345, 591, 725
GsaI CCCAGC 1 cut(s) 383
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 4 cut(s) 119, 651, 782, 812
HapII CCGG 1 cut(s) 111
HhaI GCGC 1 cut(s) 295
Hin1II CATG 3 cut(s) 338, 344, 731
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 221, 370
HpaII CCGG 1 cut(s) 111
HphI GGTGA 4 cut(s) 79, 115, 539, 776
Hpy166II GTNNAC 3 cut(s) 214, 474, 772
Hpy188I TCNGA 2 cut(s) 57, 76
Hpy188III TCNNGA 4 cut(s) 169, 232, 244, 456
Hpy8I GTNNAC 3 cut(s) 214, 474, 772
HpyAV CCTTC 2 cut(s) 250, 428
HpyCH4III ACNGT 4 cut(s) 196, 542, 643, 676
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 5 cut(s) 344, 422, 431, 562, 724
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 116, 428
HpyF3I CTNAG 1 cut(s) 687
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 3 cut(s) 338, 344, 731
HspAI GCGC 1 cut(s) 293
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 6 cut(s) 49, 76, 246, 502, 571, 603
LmnI GCTCC 2 cut(s) 77, 713
Lsp1109I GCAGC 3 cut(s) 356, 577, 736
LweI GCATC 3 cut(s) 97, 498, 560
MaeI CTAG 2 cut(s) 609, 710
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 2 cut(s) 349, 527
MalI GATC 6 cut(s) 51, 78, 248, 504, 573, 605
MboI GATC 6 cut(s) 49, 76, 246, 502, 571, 603
MboII GAAGA 3 cut(s) 44, 151, 592
MflI RGATCY 2 cut(s) 49, 571
MhlI GDGCHC 1 cut(s) 718
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 3 cut(s) 83, 483, 519
MluNI TGGCCA 1 cut(s) 812
MmeI TCCRAC 2 cut(s) 297, 437
MnlI CCTC 7 cut(s) 131, 136, 140, 313, 728, 760, 830
Mox20I TGGCCA 1 cut(s) 812
MroNI GCCGGC 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 635
MscI TGGCCA 1 cut(s) 812
MslI CAYNNNNRTG 2 cut(s) 339, 677
Msp20I TGGCCA 1 cut(s) 812
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 15, 799
MvaI CCWGG 2 cut(s) 15, 799
MwoI GCNNNNNNNGC 3 cut(s) 111, 116, 428
NaeI GCCGGC 1 cut(s) 112
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 49, 76, 246, 502, 571, 603
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 3 cut(s) 338, 344, 731
NlaIV GGNNCC 3 cut(s) 12, 650, 715
NmuCI GTSAC 2 cut(s) 349, 527
OliI CACNNNNGTG 1 cut(s) 677
PceI AGGCCT 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PdiI GCCGGC 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 2 cut(s) 221, 370
PkrI GCNGC 3 cut(s) 346, 592, 726
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 13, 797
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 2 cut(s) 13, 797
PspN4I GGNNCC 3 cut(s) 12, 650, 715
PspPI GGNCC 3 cut(s) 11, 118, 649
PspPPI RGGWCCY 1 cut(s) 11
PstI CTGCAG 1 cut(s) 726
PsuI RGATCY 2 cut(s) 49, 571
RsaI GTAC 2 cut(s) 185, 329
RsaNI GTAC 2 cut(s) 184, 328
RseI CAYNNNNRTG 2 cut(s) 339, 677
SatI GCNGC 3 cut(s) 345, 591, 725
Sau3AI GATC 6 cut(s) 49, 76, 246, 502, 571, 603
Sau96I GGNCC 3 cut(s) 11, 118, 649
ScaI AGTACT 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 15, 799
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 3 cut(s) 97, 498, 560
SfcI CTRYAG 6 cut(s) 67, 100, 276, 354, 675, 722
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 339, 677
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 3 cut(s) 83, 483, 519
SseBI AGGCCT 1 cut(s) 782
SspMI CTAG 2 cut(s) 609, 710
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 2 cut(s) 13, 797
StyI CCWWGG 3 cut(s) 60, 777, 783
TaaI ACNGT 4 cut(s) 196, 542, 643, 676
TaiI ACGT 1 cut(s) 455
TasI AATT 3 cut(s) 83, 483, 519
TatI WGTACW 2 cut(s) 183, 327
TfiI GAWTC 2 cut(s) 221, 370
TscAI CASTG 4 cut(s) 141, 648, 679, 753
TseFI GTSAC 2 cut(s) 349, 527
TseI GCWGC 3 cut(s) 344, 590, 724
Tsp45I GTSAC 2 cut(s) 349, 527
TspDTI ATGAA 2 cut(s) 273, 744
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 4 cut(s) 141, 648, 679, 753
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XmiI GTMKAC 2 cut(s) 473, 771
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 2 cut(s) 609, 710
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.