MD02G1185100.v1.1

Mitochondrial outer membrane protein porin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003645
Physical Location & Seq
Forward (+)
16803844 .. 16807653
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1185100.v1.1.491

Sequence Viewer

Length: 831 bp
ATGAGCAAGGGACCTGGACTTTTCACCGACATCGGCAAAAAAGCCAAAGATCTTCTGACCAAGGACTACAGCTCCGATCAGAAATTCACCGTCTCCACCTACAGCGATGCGGGAGTGGCCCTCACCTCAACAGCAGTGAAGAAAGGAGGACTTTCCACTGGGGACATCTTGACACAATACAAGTACAAGAACACGGTGGTTGATGTCAAAGTTGATACAGAATCAAATATCTCAACGACCTTCACGATCAATGAGATTGTCCCATCCACAAAAACTATAGCTTCATTCAAAGCCCCTGATTTCAACTCTGGCAAGTTGGAGGTTCAATACTTCCATGACCATGCAACCTTCACTACAGCTGTTGCCTTGAGTCAATCCCCAGCCATTGATTTTTCAGCCACCATTGGAACCCCAACCATTGCATTTGGTGCAGAAGGGGGTTATGACACTACCTCGGGAAACTTTACAAAGTACACTGCTGGAATTAGTATCACAAAGCCCGATCAGTCTGCTTCAATAATTTTGGGCGACAAGGGAGATAGTATAAGAGCATCTTATGTGCATTATATGGATTTGTTGAAGAGGAGTGCTGCTGTGGGAGAGATCACAAGAAAGTTCTCCACGAACGAGAACACATTCACGGTGGGAGGACAATATGCTGTTGATATTTACACAATAGTGAAAGCTAAGCTCAACAATCACGGAAAGCTAGGAGCCCTCCTGCAGCATGAAGTCATACCAAAGTCGGTGCTGACAATATCTGGCGAGGTTGACACCAAGGCCTTGGACAAAAATCCCAGGTTTGGAGTGGCTATTGCCCTGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Porin_3

Protein Analysis

277

Amino Acids

29.48

Weight (kDa)

8.76

Isoelectric Point (pI)

19.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 4 - 269 6.9e-76 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 2 cut(s) 185, 473
AfiI CCNNNNNNNGG 1 cut(s) 803
AgsI TTSAA 5 cut(s) 289, 304, 326, 516, 580
AjnI CCWGG 2 cut(s) 13, 797
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 6 cut(s) 72, 281, 359, 686, 691, 709
AluI AGCT 6 cut(s) 72, 281, 359, 686, 691, 709
Alw26I GTCTC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 454
AoxI GGCC 2 cut(s) 117, 780
ApeKI GCWGC 2 cut(s) 590, 724
ApoI RAATTY 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 635
AspS9I GGNCC 2 cut(s) 11, 118
AsuHPI GGTGA 3 cut(s) 16, 79, 115
AvaI CYCGRG 1 cut(s) 454
AvaII GGWCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 718
BbvI GCAGC 2 cut(s) 577, 736
BccI CCATC 1 cut(s) 271
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 2 cut(s) 15, 799
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 5 cut(s) 67, 100, 276, 354, 722
BglII AGATCT 1 cut(s) 49
BisI GCNGC 2 cut(s) 591, 725
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 2 cut(s) 592, 726
Bme1390I CCNGG 2 cut(s) 15, 799
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 11, 118
BmiI GGNNCC 3 cut(s) 12, 409, 715
BmrFI CCNGG 2 cut(s) 15, 799
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 97, 560
BmuI ACTGGG 1 cut(s) 168
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu1102I GCTNAGC 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 388
BsaJI CCNNGG 5 cut(s) 60, 453, 777, 783, 797
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse1I ACTGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 2 cut(s) 15, 799
BseDI CCNNGG 5 cut(s) 60, 453, 777, 783, 797
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 598
BseXI GCAGC 2 cut(s) 577, 736
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 2 cut(s) 119, 782
BsiHKCI CYCGRG 1 cut(s) 454
BslFI GGGAC 3 cut(s) 24, 176, 245
BslI CCNNNNNNNGG 1 cut(s) 803
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 24, 176, 245
BsnI GGCC 2 cut(s) 119, 782
BsoBI CYCGRG 1 cut(s) 454
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 5 cut(s) 49, 76, 246, 502, 603
Bsp1720I GCTNAGC 1 cut(s) 687
BspACI CCGC 1 cut(s) 110
BspANI GGCC 2 cut(s) 119, 782
BspLI GGNNCC 3 cut(s) 12, 409, 715
BspMAI CTGCAG 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 5 cut(s) 60, 453, 777, 783, 797
BssMI GATC 5 cut(s) 49, 76, 246, 502, 603
BssT1I CCWWGG 3 cut(s) 60, 777, 783
Bst2UI CCWGG 2 cut(s) 15, 799
Bst4CI ACNGT 3 cut(s) 91, 196, 643
Bst6I CTCTTC 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 428
BstDEI CTNAG 1 cut(s) 687
BstF5I GGATG 1 cut(s) 263
BstKTI GATC 5 cut(s) 52, 79, 249, 505, 606
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 5 cut(s) 49, 76, 246, 502, 603
BstMWI GCNNNNNNNGC 2 cut(s) 116, 428
BstNI CCWGG 2 cut(s) 15, 799
BstSCI CCNGG 2 cut(s) 13, 797
BstSFI CTRYAG 5 cut(s) 67, 100, 276, 354, 722
BstV1I GCAGC 2 cut(s) 577, 736
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 2 cut(s) 119, 782
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 263
BtsI GCAGTG 2 cut(s) 141, 474
BtsIMutI CAGTG 3 cut(s) 141, 156, 474
Cfr13I GGNCC 2 cut(s) 11, 118
Csp6I GTAC 2 cut(s) 184, 472
CviAII CATG 3 cut(s) 335, 341, 728
CviQI GTAC 2 cut(s) 184, 472
DdeI CTNAG 1 cut(s) 687
DpnI GATC 5 cut(s) 51, 78, 248, 504, 605
DpnII GATC 5 cut(s) 49, 76, 246, 502, 603
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 60, 777, 783
Eco147I AGGCCT 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 11
Eco88I CYCGRG 1 cut(s) 454
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 13, 797
EcoT14I CCWWGG 3 cut(s) 60, 777, 783
EcoT38I GRGCYC 1 cut(s) 718
ErhI CCWWGG 3 cut(s) 60, 777, 783
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 3 cut(s) 338, 344, 731
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 538, 570
FaqI GGGAC 3 cut(s) 24, 176, 245
FatI CATG 3 cut(s) 334, 340, 727
FauI CCCGC 1 cut(s) 103
Fnu4HI GCNGC 2 cut(s) 591, 725
FokI GGATG 1 cut(s) 250
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 2 cut(s) 591, 725
FspBI CTAG 1 cut(s) 710
GluI GCNGC 2 cut(s) 591, 725
GsaI CCCAGC 1 cut(s) 383
HaeIII GGCC 2 cut(s) 119, 782
Hin1II CATG 3 cut(s) 338, 344, 731
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 2 cut(s) 221, 370
HphI GGTGA 3 cut(s) 16, 79, 115
Hpy166II GTNNAC 2 cut(s) 474, 772
Hpy188I TCNGA 3 cut(s) 57, 76, 81
Hpy188III TCNNGA 3 cut(s) 169, 244, 456
Hpy8I GTNNAC 2 cut(s) 474, 772
HpyAV CCTTC 3 cut(s) 250, 358, 428
HpyCH4III ACNGT 3 cut(s) 91, 196, 643
HpyCH4V TGCA 5 cut(s) 344, 422, 431, 562, 724
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 428
HpyF3I CTNAG 1 cut(s) 687
Hsp92II CATG 3 cut(s) 338, 344, 731
Kzo9I GATC 5 cut(s) 49, 76, 246, 502, 603
LmnI GCTCC 2 cut(s) 77, 713
Lsp1109I GCAGC 2 cut(s) 577, 736
LweI GCATC 2 cut(s) 97, 560
MaeI CTAG 1 cut(s) 710
MalI GATC 5 cut(s) 51, 78, 248, 504, 605
MboI GATC 5 cut(s) 49, 76, 246, 502, 603
MboII GAAGA 3 cut(s) 44, 151, 592
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 3 cut(s) 83, 483, 519
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 9 cut(s) 131, 136, 140, 313, 463, 576, 641, 728, 760
MroXI GAANNNNTTC 1 cut(s) 635
MslI CAYNNNNRTG 2 cut(s) 339, 677
MspA1I CMGCKG 1 cut(s) 359
MspR9I CCNGG 2 cut(s) 15, 799
MvaI CCWGG 2 cut(s) 15, 799
MwoI GCNNNNNNNGC 2 cut(s) 116, 428
NdeII GATC 5 cut(s) 49, 76, 246, 502, 603
NlaIII CATG 3 cut(s) 338, 344, 731
NlaIV GGNNCC 3 cut(s) 12, 409, 715
OliI CACNNNNGTG 1 cut(s) 677
PceI AGGCCT 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 592, 726
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 13, 797
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 2 cut(s) 13, 797
PspN4I GGNNCC 3 cut(s) 12, 409, 715
PspPI GGNCC 2 cut(s) 11, 118
PspPPI RGGWCCY 1 cut(s) 11
PstI CTGCAG 1 cut(s) 726
PsuI RGATCY 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 2 cut(s) 185, 473
RsaNI GTAC 2 cut(s) 184, 472
RseI CAYNNNNRTG 2 cut(s) 339, 677
SatI GCNGC 2 cut(s) 591, 725
Sau3AI GATC 5 cut(s) 49, 76, 246, 502, 603
Sau96I GGNCC 2 cut(s) 11, 118
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 15, 799
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 2 cut(s) 97, 560
SfcI CTRYAG 5 cut(s) 67, 100, 276, 354, 722
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 339, 677
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
Sse9I AATT 3 cut(s) 83, 483, 519
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 1 cut(s) 110
SspMI CTAG 1 cut(s) 710
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 2 cut(s) 13, 797
StyI CCWWGG 3 cut(s) 60, 777, 783
TaaI ACNGT 3 cut(s) 91, 196, 643
TasI AATT 3 cut(s) 83, 483, 519
TatI WGTACW 2 cut(s) 183, 471
TfiI GAWTC 1 cut(s) 221
TscAI CASTG 3 cut(s) 141, 163, 481
TseI GCWGC 2 cut(s) 590, 724
TspDTI ATGAA 2 cut(s) 273, 744
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 3 cut(s) 141, 163, 481
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.