Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0005858

Physical Location & Seq

Genomic Coordinates Reverse (-)

2248299 .. 2252454

Gene Structure 4156 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g04250.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGTTGCTTCTGCTGCTACTTCGTCGTTCTTTCCGGTTCCTTCTCCTGACTCTGGTGCCAAGAATTCCAAGAATGGAAATGGGAATTCCAAGTTAGGAATCAAATCAAAATCTGCTGCGGCTGGTTTGCAGGTAAAGGCCAACGCTCAAGCCCCTCCAAAAATAAATGGCACTAGTGTTGGTTTGGCCACTGTTGAAAGTGGAAAGCATGGGGATGACATGCACGGACTTACTCAATCCCCTCCTGCACGGACTTTCATTAACCAATTACCTGATTGGAGTATGCTCCTTGCTGCTATAACTACAATCTTCTTGGCAGCTGAAAAACAATGGATGATGCTTGATTGGAAACCCAAGCGGCCTGATATGCTAATTGACCCATTTGGTCTGGGAAGAATTGTTCAGGATGGTCTTGTATTCCGCCAGAACTTCTCCATTAGATCGTATGAAATAGGTGCTGATAGAACGGCTTCAATCGAGACGTTAATGAATCATTTACAGGAAACAGCGCTTAATCATGTTAAAACTGCTGGCCTCTTAGGGGACGGCTTTGGTTCAACTCCAGAGATGTCCATAAGAAACCTTATATGGGTGGTAACCAAAATGCAGGTTAAGGTAGACCGCTATCCTACTTGGGGTGATGTTGTTCAAGTTGACACTTGGGTTAGTGCCTCTGGGAAGAATGGTATGCGGCGTGATTGGATTATCCAGGATTGCAAAACAGGACAAATATTAACAAGAGCCTCCAGTGTGTGGGTGATGATGAATAAACTGACTAGGAGGTTATCCAAGATACCCAATGAGGTTCGAAGTGAAATAGAGCCTTACTTTGTGAATAATCCTCCTGTTGTGGAGGAGGATGGCAGGAAACTGCAGAAACTTGACGACAATACAGCGGACTTTGTCCGTACTGGTTTAACTCCTCGGTGGAGTGATCTGGACATCAACCAGCATGTTAACAATGTCAAGTACATTGGCTGGATCCTTGAGAGTGCTCCCTTGCCAATCTTGGAGAGCCATGAGCTTTCTTCAATGACTCTGGAGTATAGGAGGGAGTGTGGTAGGGACAGTGTGCTTCAGTCCCTGTCTGCAGTTTCTGCTGATATCGGGCATTTGGCAAGCACTGGCAACGTAGAGTGCCAGCACTTGCTTCGACTCGAGGGTGGGTCTGAGATTGTGAGGGGAAGGACTGGTTGGAGGCCCAAAAAACCCAACAATCTCGGGATTATGGGTGGGTTTCCAGCTGAAAGCGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000036 GO:0003674 GO:0003824 GO:0004312 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016297 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044620 GO:0046394 GO:0048037 GO:0051192 GO:0071704 GO:0072330 GO:0140104 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko01100 ko01212 map00061 map01100 map01212

Pfam Domains

Protein Families

Acyl-ACP_TE Acyl-thio_N

Protein Analysis

419

Amino Acids

45.97

Weight (kDa)

8.83

Isoelectric Point (pI)

41.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-thio_N	PF12590	1 - 126	5.4e-52	Acyl-ATP thioesterase
Acyl-ACP_TE	PF01643	137 - 273	1e-41	Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C	PF20791	299 - 400	1.4e-27	Acyl-ACP thioesterase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005858)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08510
fragaria_vesca	FvH4_1g04250 FvH4_1g04250 FvH4_1g04250 FvH4_6g30873
malus_domestica	MD02G1042500.v1.1 MD02G1062000.v1.1 MD09G1215700.v1.1
prunus_persica	Prupe.3G074600_v2.0.a1 Prupe.3G074600_v2.0.a1 Prupe.3G074700_v2.0.a1 Prupe.7G234600_v2.0.a1 Prupe.7G234600_v2.0.a1 Prupe.7G234600_v2.0.a1
pyrus_communis	pycom02g03520 pycom15g16310
rosa_chinensis	RchiOBHm_Chr2g0089811
rosa_laevigata	RLG00000016057
rosa_multiflora	Rmu_sc0000665.1_g000007
rosa_roxburghii	Rroxscaffold_2G00151600
rosa_rugosa	Rorug02G0003400 Rorug02G0003500
rosa_samantha	Rh2AG048600 Rh2AG389900 Rh2BG047400 Rh2CG049500 Rh2CG377400
rosa_wichuraiana	Rw2G004180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	121, 598
AccB1I	GGYRCC	1 cut(s)	56
AccB7I	CCANNNNNTGG	1 cut(s)	753
AccI	GTMKAC	1 cut(s)	618
AciI	CCGC	6 cut(s)	119, 358, 421, 622, 691, 896
AclWI	GGATC	2 cut(s)	976, 989
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	2 cut(s)	64, 85
AcuI	CTGAAG	1 cut(s)	1061
AfaI	GTAC	2 cut(s)	910, 971
AfeI	AGCGCT	1 cut(s)	510
AfiI	CCNNNNNNNGG	5 cut(s)	53, 541, 589, 635, 753
AgsI	TTSAA	5 cut(s)	197, 474, 558, 650, 1032
AhlI	ACTAGT	1 cut(s)	173
AjnI	CCWGG	1 cut(s)	708
AjuI	GAANNNNNNNTTGG	2 cut(s)	1177, 1209
AloI	GAACNNNNNNTCC	2 cut(s)	384, 416
AluBI	AGCT	3 cut(s)	320, 1024, 1244
AluI	AGCT	3 cut(s)	320, 1024, 1244
Alw21I	GWGCWC	1 cut(s)	997
Alw26I	GTCTC	1 cut(s)	473
AlwI	GGATC	2 cut(s)	976, 989
AlwNI	CAGNNNCTG	2 cut(s)	1084, 1097
Ama87I	CYCGRG	2 cut(s)	1157, 1220
Aor51HI	AGCGCT	1 cut(s)	510
AoxI	GGCC	5 cut(s)	138, 186, 359, 532, 1199
ApeKI	GCWGC	4 cut(s)	14, 116, 293, 317
ApoI	RAATTY	2 cut(s)	64, 85
Asp700I	GAANNNNTTC	1 cut(s)	469
AspLEI	GCGC	2 cut(s)	511, 1253
AspS9I	GGNCC	1 cut(s)	1200
AsuHPI	GGTGA	2 cut(s)	650, 769
AsuII	TTCGAA	1 cut(s)	808
AvaI	CYCGRG	2 cut(s)	1157, 1220
BalI	TGGCCA	1 cut(s)	188
BamHI	GGATCC	1 cut(s)	981
BanI	GGYRCC	1 cut(s)	56
Bbv12I	GWGCWC	1 cut(s)	997
BbvI	GCAGC	3 cut(s)	103, 280, 329
BccI	CCATC	2 cut(s)	401, 854
BceAI	ACGGC	2 cut(s)	483, 562
BcgI	CGANNNNNNTGC	2 cut(s)	1133, 1167
BciT130I	CCWGG	1 cut(s)	710
BcoDI	GTCTC	1 cut(s)	473
BcuI	ACTAGT	1 cut(s)	173
BfaI	CTAG	2 cut(s)	174, 777
BfmI	CTRYAG	2 cut(s)	872, 1089
BfoI	RGCGCY	1 cut(s)	512
BfuAI	ACCTGC	2 cut(s)	121, 598
BisI	GCNGC	7 cut(s)	15, 117, 120, 294, 318, 359, 692
BlsI	GCNGC	7 cut(s)	16, 118, 121, 295, 319, 360, 693
Bme1390I	CCNGG	1 cut(s)	710
BmeT110I	CYCGRG	2 cut(s)	1157, 1220
BmgT120I	GGNCC	1 cut(s)	1200
BmiI	GGNNCC	3 cut(s)	39, 58, 983
BmrFI	CCNGG	1 cut(s)	710
BmsI	GCATC	1 cut(s)	327
BpmI	CTGGAG	3 cut(s)	546, 730, 1061
Bpu14I	TTCGAA	1 cut(s)	808
BpuEI	CTTGAG	2 cut(s)	132, 1007
BsaJI	CCNNGG	1 cut(s)	923
BsaWI	WCCGGW	1 cut(s)	34
BsaXI	ACNNNNNCTCC	2 cut(s)	1042, 1072
Bsc4I	CCNNNNNNNGG	5 cut(s)	53, 541, 589, 635, 753
Bse1I	ACTGG	4 cut(s)	747, 916, 1129, 1195
BseBI	CCWGG	1 cut(s)	710
BseDI	CCNNGG	1 cut(s)	923
BseGI	GGATG	4 cut(s)	220, 339, 412, 865
BseLI	CCNNNNNNNGG	5 cut(s)	53, 541, 589, 635, 753
BseMII	CTCAG	1 cut(s)	1161
BseNI	ACTGG	4 cut(s)	747, 916, 1129, 1195
BseRI	GAGGAG	2 cut(s)	869, 912
BseXI	GCAGC	3 cut(s)	103, 280, 329
BsgI	GTGCAG	1 cut(s)	231
BshFI	GGCC	5 cut(s)	140, 188, 361, 534, 1201
BshNI	GGYRCC	1 cut(s)	56
BsiHKAI	GWGCWC	1 cut(s)	997
BsiHKCI	CYCGRG	2 cut(s)	1157, 1220
BsiSI	CCGG	1 cut(s)	35
BslFI	GGGAC	3 cut(s)	557, 1066, 1079
BslI	CCNNNNNNNGG	5 cut(s)	53, 541, 589, 635, 753
BsmAI	GTCTC	1 cut(s)	473
BsmBI	CGTCTC	1 cut(s)	473
BsmFI	GGGAC	3 cut(s)	557, 1066, 1079
BsnI	GGCC	5 cut(s)	140, 188, 361, 534, 1201
BsoBI	CYCGRG	2 cut(s)	1157, 1220
Bsp119I	TTCGAA	1 cut(s)	808
Bsp1286I	GDGCHC	1 cut(s)	997
Bsp143I	GATC	3 cut(s)	440, 934, 981
BspACI	CCGC	6 cut(s)	119, 358, 421, 622, 691, 896
BspANI	GGCC	5 cut(s)	140, 188, 361, 534, 1201
BspCNI	CTCAG	1 cut(s)	1162
BspLI	GGNNCC	3 cut(s)	39, 58, 983
BspMAI	CTGCAG	2 cut(s)	876, 1093
BspMI	ACCTGC	2 cut(s)	121, 598
BspPI	GGATC	2 cut(s)	976, 989
BspT104I	TTCGAA	1 cut(s)	808
BspT107I	GGYRCC	1 cut(s)	56
BsrI	ACTGG	4 cut(s)	747, 916, 1129, 1195
BssECI	CCNNGG	1 cut(s)	923
BssMI	GATC	3 cut(s)	440, 934, 981
Bst2UI	CCWGG	1 cut(s)	710
Bst4CI	ACNGT	2 cut(s)	193, 1070
BstAPI	GCANNNNNTGC	1 cut(s)	1097
BstBI	TTCGAA	1 cut(s)	808
BstC8I	GCNNGC	3 cut(s)	532, 1120, 1142
BstDEI	CTNAG	2 cut(s)	538, 1170
BstEII	GGTNACC	1 cut(s)	595
BstF5I	GGATG	4 cut(s)	220, 339, 412, 865
BstH2I	RGCGCY	1 cut(s)	512
BstHHI	GCGC	2 cut(s)	511, 1253
BstKTI	GATC	3 cut(s)	443, 937, 984
BstMAI	GTCTC	1 cut(s)	473
BstMBI	GATC	3 cut(s)	440, 934, 981
BstMWI	GCNNNNNNNGC	4 cut(s)	14, 367, 1097, 1250
BstNI	CCWGG	1 cut(s)	710
BstNSI	RCATGY	2 cut(s)	223, 956
BstPI	GGTNACC	1 cut(s)	595
BstSCI	CCNGG	1 cut(s)	708
BstSFI	CTRYAG	2 cut(s)	872, 1089
BstV1I	GCAGC	3 cut(s)	103, 280, 329
BstX2I	RGATCY	1 cut(s)	981
BstYI	RGATCY	1 cut(s)	981
BsuRI	GGCC	5 cut(s)	140, 188, 361, 534, 1201
BtsCI	GGATG	4 cut(s)	220, 339, 412, 865
BtsIMutI	CAGTG	4 cut(s)	189, 754, 1075, 1122
BveI	ACCTGC	2 cut(s)	121, 598
Cac8I	GCNNGC	3 cut(s)	532, 1120, 1142
CaiI	CAGNNNCTG	2 cut(s)	1084, 1097
CfoI	GCGC	2 cut(s)	511, 1253
Cfr13I	GGNCC	1 cut(s)	1200
Csp6I	GTAC	2 cut(s)	909, 970
CviAII	CATG	5 cut(s)	209, 220, 518, 953, 1019
CviQI	GTAC	2 cut(s)	909, 970
DdeI	CTNAG	2 cut(s)	538, 1170
DpnI	GATC	3 cut(s)	442, 936, 983
DpnII	GATC	3 cut(s)	440, 934, 981
EaeI	YGGCCR	1 cut(s)	186
EciI	GGCGGA	1 cut(s)	410
Eco32I	GATATC	1 cut(s)	1105
Eco47III	AGCGCT	1 cut(s)	510
Eco57I	CTGAAG	1 cut(s)	1061
Eco88I	CYCGRG	2 cut(s)	1157, 1220
Eco91I	GGTNACC	1 cut(s)	595
EcoO65I	GGTNACC	1 cut(s)	595
EcoRI	GAATTC	2 cut(s)	64, 85
EcoRII	CCWGG	1 cut(s)	708
EcoRV	GATATC	1 cut(s)	1105
Esp3I	CGTCTC	1 cut(s)	473
FaeI	CATG	5 cut(s)	212, 223, 521, 956, 1022
FaqI	GGGAC	3 cut(s)	557, 1066, 1079
FatI	CATG	5 cut(s)	208, 219, 517, 952, 1018
FblI	GTMKAC	1 cut(s)	618
Fnu4HI	GCNGC	7 cut(s)	15, 117, 120, 294, 318, 359, 692
FokI	GGATG	4 cut(s)	227, 346, 419, 872
Fsp4HI	GCNGC	7 cut(s)	15, 117, 120, 294, 318, 359, 692
FspBI	CTAG	2 cut(s)	174, 777
GlaI	GCGC	2 cut(s)	510, 1252
GluI	GCNGC	7 cut(s)	15, 117, 120, 294, 318, 359, 692
GsuI	CTGGAG	3 cut(s)	546, 730, 1061
HaeII	RGCGCY	1 cut(s)	512
HaeIII	GGCC	5 cut(s)	140, 188, 361, 534, 1201
HapII	CCGG	1 cut(s)	35
HhaI	GCGC	2 cut(s)	511, 1253
Hin1II	CATG	5 cut(s)	212, 223, 521, 956, 1022
Hin6I	GCGC	2 cut(s)	509, 1251
HinP1I	GCGC	2 cut(s)	509, 1251
HincII	GTYRAC	2 cut(s)	655, 958
HindII	GTYRAC	2 cut(s)	655, 958
HinfI	GANTC	5 cut(s)	50, 99, 490, 1036, 1155
HpaI	GTTAAC	1 cut(s)	958
HpaII	CCGG	1 cut(s)	35
HphI	GGTGA	2 cut(s)	650, 769
Hpy166II	GTNNAC	3 cut(s)	619, 655, 958
Hpy188I	TCNGA	1 cut(s)	1171
Hpy188III	TCNNGA	7 cut(s)	47, 404, 478, 563, 938, 1040, 1222
Hpy8I	GTNNAC	3 cut(s)	619, 655, 958
Hpy99I	CGWCG	1 cut(s)	28
HpyAV	CCTTC	2 cut(s)	51, 1179
HpyCH4III	ACNGT	2 cut(s)	193, 1070
HpyCH4IV	ACGT	2 cut(s)	482, 1131
HpyCH4V	TGCA	7 cut(s)	130, 223, 248, 607, 717, 874, 1091
HpyF10VI	GCNNNNNNNGC	4 cut(s)	14, 367, 1097, 1250
HpyF3I	CTNAG	2 cut(s)	538, 1170
HpySE526I	ACGT	2 cut(s)	482, 1131
Hsp92II	CATG	5 cut(s)	212, 223, 521, 956, 1022
HspAI	GCGC	2 cut(s)	509, 1251
KspAI	GTTAAC	1 cut(s)	958
Kzo9I	GATC	3 cut(s)	440, 934, 981
LmnI	GCTCC	2 cut(s)	291, 1000
Lsp1109I	GCAGC	3 cut(s)	103, 280, 329
LweI	GCATC	1 cut(s)	327
MaeI	CTAG	2 cut(s)	174, 777
MaeII	ACGT	2 cut(s)	482, 1131
MaeIII	GTNAC	1 cut(s)	595
MalI	GATC	3 cut(s)	442, 936, 983
MboI	GATC	3 cut(s)	440, 934, 981
MboII	GAAGA	4 cut(s)	301, 405, 691, 1020
MflI	RGATCY	1 cut(s)	981
MhlI	GDGCHC	1 cut(s)	997
MlsI	TGGCCA	1 cut(s)	188
MluCI	AATT	5 cut(s)	64, 85, 266, 372, 396
MluNI	TGGCCA	1 cut(s)	188
MlyI	GAGTC	3 cut(s)	44, 1030, 1149
MmeI	TCCRAC	1 cut(s)	1175
Mox20I	TGGCCA	1 cut(s)	188
MroXI	GAANNNNTTC	1 cut(s)	469
MscI	TGGCCA	1 cut(s)	188
MseI	TTAA	8 cut(s)	261, 485, 513, 522, 612, 734, 917, 957
MslI	CAYNNNNRTG	1 cut(s)	213
Msp20I	TGGCCA	1 cut(s)	188
MspA1I	CMGCKG	3 cut(s)	320, 896, 1244
MspI	CCGG	1 cut(s)	35
MspR9I	CCNGG	1 cut(s)	710
MvaI	CCWGG	1 cut(s)	710
MwoI	GCNNNNNNNGC	4 cut(s)	14, 367, 1097, 1250
NdeII	GATC	3 cut(s)	440, 934, 981
NlaIII	CATG	5 cut(s)	212, 223, 521, 956, 1022
NlaIV	GGNNCC	3 cut(s)	39, 58, 983
NspI	RCATGY	2 cut(s)	223, 956
NspV	TTCGAA	1 cut(s)	808
PaeR7I	CTCGAG	1 cut(s)	1157
PdmI	GAANNNNTTC	1 cut(s)	469
PfeI	GAWTC	2 cut(s)	99, 490
PflFI	GACNNNGTC	1 cut(s)	902
PflMI	CCANNNNNTGG	1 cut(s)	753
PfoI	TCCNGGA	1 cut(s)	708
PkrI	GCNGC	7 cut(s)	16, 118, 121, 295, 319, 360, 693
PleI	GAGTC	3 cut(s)	44, 1030, 1149
PpsI	GAGTC	3 cut(s)	44, 1030, 1149
Psp6I	CCWGG	1 cut(s)	708
PspEI	GGTNACC	1 cut(s)	595
PspGI	CCWGG	1 cut(s)	708
PspN4I	GGNNCC	3 cut(s)	39, 58, 983
PspPI	GGNCC	1 cut(s)	1200
PspXI	VCTCGAGB	1 cut(s)	1157
PstI	CTGCAG	2 cut(s)	876, 1093
PstNI	CAGNNNCTG	2 cut(s)	1084, 1097
PsuI	RGATCY	1 cut(s)	981
PsyI	GACNNNGTC	1 cut(s)	902
PvuII	CAGCTG	2 cut(s)	320, 1244
RsaI	GTAC	2 cut(s)	910, 971
RsaNI	GTAC	2 cut(s)	909, 970
RseI	CAYNNNNRTG	1 cut(s)	213
SaqAI	TTAA	8 cut(s)	261, 485, 513, 522, 612, 734, 917, 957
SatI	GCNGC	7 cut(s)	15, 117, 120, 294, 318, 359, 692
Sau3AI	GATC	3 cut(s)	440, 934, 981
Sau96I	GGNCC	1 cut(s)	1200
SchI	GAGTC	3 cut(s)	44, 1030, 1149
ScrFI	CCNGG	1 cut(s)	710
SduI	GDGCHC	1 cut(s)	997
SfaNI	GCATC	1 cut(s)	327
SfcI	CTRYAG	2 cut(s)	872, 1089
Sfr274I	CTCGAG	1 cut(s)	1157
SfuI	TTCGAA	1 cut(s)	808
SlaI	CTCGAG	1 cut(s)	1157
SmiMI	CAYNNNNRTG	1 cut(s)	213
SmlI	CTYRAG	3 cut(s)	147, 986, 1157
SmoI	CTYRAG	3 cut(s)	147, 986, 1157
SpeI	ACTAGT	1 cut(s)	173
Sse9I	AATT	5 cut(s)	64, 85, 266, 372, 396
SsiI	CCGC	6 cut(s)	119, 358, 421, 622, 691, 896
SspI	AATATT	1 cut(s)	732
SspMI	CTAG	2 cut(s)	174, 777
StyD4I	CCNGG	1 cut(s)	708
TaaI	ACNGT	2 cut(s)	193, 1070
TaiI	ACGT	2 cut(s)	485, 1134
TaqI	TCGA	4 cut(s)	477, 808, 1153, 1158
TasI	AATT	5 cut(s)	64, 85, 266, 372, 396
TatI	WGTACW	1 cut(s)	969
TauI	GCSGC	3 cut(s)	122, 361, 694
TfiI	GAWTC	2 cut(s)	99, 490
Tru1I	TTAA	8 cut(s)	261, 485, 513, 522, 612, 734, 917, 957
Tru9I	TTAA	8 cut(s)	261, 485, 513, 522, 612, 734, 917, 957
TscAI	CASTG	4 cut(s)	196, 754, 1075, 1129
TseI	GCWGC	4 cut(s)	14, 116, 293, 317
TspDTI	ATGAA	4 cut(s)	247, 462, 503, 779
TspGWI	ACGGA	3 cut(s)	240, 265, 896
TspRI	CASTG	4 cut(s)	196, 754, 1075, 1129
Tth111I	GACNNNGTC	1 cut(s)	902
Van91I	CCANNNNNTGG	1 cut(s)	753
XapI	RAATTY	2 cut(s)	64, 85
XceI	RCATGY	2 cut(s)	223, 956
XhoI	CTCGAG	1 cut(s)	1157
XmiI	GTMKAC	1 cut(s)	618
XmnI	GAANNNNTTC	1 cut(s)	469
XspI	CTAG	2 cut(s)	174, 777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.