Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005858

Physical Location & Seq

Genomic Coordinates Reverse (-)

3484770 .. 3489451

Gene Structure 4682 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1042500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGTTGCCACTTCTGCTACTGCCTCGTTCTTTCCGGTTTCTTCTCCCAACTCAGACTCAAGCGCCAAGAACGCCAAGCTTGGGTCAGCCAATTTAGGACTCAAATCGAAGTCTGCATCTGGTGGTTTGCAGGTAAAGGCAAATGCTCAAGCCCCTTCAAAGATAAATGGAACTAGTGTTGGTTTGGCAACCGTGGAAAGTGGGAAGCATGGGGATGACATTTCATCCCCTCCGGCACGGACTTTCATTAACCAATTACCTGATTGGAGTGTGCTCCTTGCTGCTATTACCACAATCTTCTTGGCTGCAGAGAAGCAATGGACAATGCTTGATTGGAAACCCAAGCGACCTGACATGCTCATTGACCCATTTGGTCTAGGACGAATTGTTCAGGATGGTCTTGTCTTTCGCCAGAACTTCTCAATTAGATCATATGAAATAGGTGCTGATCGTACGGCTTCAATAGAGACGTTAATGAATCATTTACAGGAAACAGCACTTAATCATGTTAAGACTGCTGGACTTCTGGGAGATGGTTTTGGTTCAACTCCAGAGATGACTGTAAGAAACCTGATATGGGTGGTAACGAAGATGCAGGTTGTGGTAGACCGCTATCCTACTTGGGGTGACGTTGTTCAAGTTGACACTTGGGTTAGTGCCTCTGGGAAGAATGGAATGCGTCGTGATTGGATTATCCAGGATTTGAAAACTGGCCAAATTCTAACAAGAGCCTCCAGTGTGTGGGTGATGATGAATAAAGTGACGAGGAGATTATCAAAGATGCCTGATGCAGTTCGCGGTGAAATAGAGTCCTTTTTTATGAATTCTCCTCCTGTTGTGGAGGAAGATGGCAGGAAACTGCCGAAACTTGATGACAAAACAGCGGACGTTGTTCTCTCTGGTTTGACTCCTAGATGGAGTGATTTAGATGTCAACCAGCACGTTAATAACGTGAAGTACATTGGCTGGATCCTTGAGGGTGCTCCCTTGCCAATCCTGGAGAGTCATGAGCTCTCTTCTTTGACTCTGGAGTATAGGAGGGAGTGCGGGAGGGACAGTGTGCTTCAGTCTCTGACTGCAGTCTCAGGTGCTGATATCGGCAACCTGGGAAGTAATGGCACGGTGGAGTGCCAGCACATGCTTCGACTTGAGGATGGGGCTGAGATTGTGAGGGGAAGGACTGAGTGGAGGCCCAAATATGCCAACAATCTTGGGATTGTGGGTCATCTTCCAGCAGAAAGCGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000036 GO:0003674 GO:0003824 GO:0004312 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016297 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044620 GO:0046394 GO:0048037 GO:0051192 GO:0071704 GO:0072330 GO:0140104 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko01100 ko01212 map00061 map01100 map01212

Pfam Domains

Protein Families

Acyl-ACP_TE Acyl-thio_N

Protein Analysis

416

Amino Acids

45.31

Weight (kDa)

6.68

Isoelectric Point (pI)

39.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-thio_N	PF12590	1 - 122	1.7e-51	Acyl-ATP thioesterase
Acyl-ACP_TE	PF01643	133 - 267	3e-40	Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C	PF20791	298 - 397	4.2e-28	Acyl-ACP thioesterase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005858)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08510
fragaria_vesca	FvH4_1g04250 FvH4_1g04250 FvH4_1g04250 FvH4_6g30873
malus_domestica	MD02G1042500.v1.1 MD02G1062000.v1.1 MD09G1215700.v1.1
prunus_persica	Prupe.3G074600_v2.0.a1 Prupe.3G074600_v2.0.a1 Prupe.3G074700_v2.0.a1 Prupe.7G234600_v2.0.a1 Prupe.7G234600_v2.0.a1 Prupe.7G234600_v2.0.a1
pyrus_communis	pycom02g03520 pycom15g16310
rosa_chinensis	RchiOBHm_Chr2g0089811
rosa_laevigata	RLG00000016057
rosa_multiflora	Rmu_sc0000665.1_g000007
rosa_roxburghii	Rroxscaffold_2G00151600
rosa_rugosa	Rorug02G0003400 Rorug02G0003500
rosa_samantha	Rh2AG048600 Rh2AG389900 Rh2BG047400 Rh2CG049500 Rh2CG377400
rosa_wichuraiana	Rw2G004180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	121, 586
AccB7I	CCANNNNNTGG	1 cut(s)	741
AccI	GTMKAC	1 cut(s)	606
AccII	CGCG	1 cut(s)	798
AciI	CCGC	4 cut(s)	610, 798, 884, 1047
AclWI	GGATC	2 cut(s)	964, 977
AcoI	YGGCCR	1 cut(s)	712
AcsI	RAATTY	2 cut(s)	717, 823
AcuI	CTGAAG	1 cut(s)	1049
AfaI	GTAC	2 cut(s)	454, 959
AfiI	CCNNNNNNNGG	4 cut(s)	81, 577, 623, 741
AgsI	TTSAA	5 cut(s)	159, 462, 546, 638, 706
AhlI	ACTAGT	1 cut(s)	173
AjnI	CCWGG	3 cut(s)	696, 996, 1104
AloI	GAACNNNNNNTCC	2 cut(s)	372, 404
AluBI	AGCT	2 cut(s)	79, 1012
AluI	AGCT	2 cut(s)	79, 1012
Alw21I	GWGCWC	3 cut(s)	276, 985, 1014
Alw26I	GTCTC	3 cut(s)	461, 1074, 1087
AlwI	GGATC	2 cut(s)	964, 977
AlwNI	CAGNNNCTG	2 cut(s)	1072, 1091
AoxI	GGCC	2 cut(s)	712, 1190
ApeKI	GCWGC	2 cut(s)	281, 305
ApoI	RAATTY	2 cut(s)	717, 823
AspLEI	GCGC	2 cut(s)	65, 1244
AspS9I	GGNCC	1 cut(s)	1191
AsuHPI	GGTGA	3 cut(s)	638, 757, 812
BalI	TGGCCA	1 cut(s)	714
BamHI	GGATCC	1 cut(s)	969
BanII	GRGCYC	1 cut(s)	1014
Bbv12I	GWGCWC	3 cut(s)	276, 985, 1014
BbvI	GCAGC	2 cut(s)	268, 292
BccI	CCATC	5 cut(s)	389, 527, 842, 909, 1148
BceAI	ACGGC	1 cut(s)	471
BcgI	CGANNNNNNTGC	2 cut(s)	1124, 1158
BciT130I	CCWGG	3 cut(s)	698, 998, 1106
BcoDI	GTCTC	3 cut(s)	461, 1074, 1087
BcuI	ACTAGT	1 cut(s)	173
BfaI	CTAG	3 cut(s)	174, 377, 912
BfmI	CTRYAG	2 cut(s)	306, 1077
BfoI	RGCGCY	1 cut(s)	66
BfuAI	ACCTGC	2 cut(s)	121, 586
BisI	GCNGC	2 cut(s)	282, 306
BlsI	GCNGC	2 cut(s)	283, 307
Bme1390I	CCNGG	3 cut(s)	698, 998, 1106
BmgT120I	GGNCC	1 cut(s)	1191
BmiI	GGNNCC	1 cut(s)	971
BmrFI	CCNGG	3 cut(s)	698, 998, 1106
BmsI	GCATC	4 cut(s)	125, 582, 771, 778
BoxI	GACNNNNGTC	1 cut(s)	1079
BpmI	CTGGAG	4 cut(s)	534, 718, 1019, 1049
BpuEI	CTTGAG	4 cut(s)	43, 132, 995, 1169
BsaJI	CCNNGG	2 cut(s)	192, 1105
BsaWI	WCCGGW	1 cut(s)	34
BsaXI	ACNNNNNCTCC	2 cut(s)	1042, 1072
Bsc4I	CCNNNNNNNGG	4 cut(s)	81, 577, 623, 741
Bse1I	ACTGG	2 cut(s)	715, 735
Bse3DI	GCAATG	1 cut(s)	323
BseBI	CCWGG	3 cut(s)	698, 998, 1106
BseDI	CCNNGG	2 cut(s)	192, 1105
BseGI	GGATG	4 cut(s)	220, 224, 400, 1159
BseLI	CCNNNNNNNGG	4 cut(s)	81, 577, 623, 741
BseMI	GCAATG	1 cut(s)	323
BseMII	CTCAG	4 cut(s)	66, 1098, 1152, 1173
BseNI	ACTGG	2 cut(s)	715, 735
BseRI	GAGGAG	2 cut(s)	781, 819
BseXI	GCAGC	2 cut(s)	268, 292
Bsh1236I	CGCG	1 cut(s)	798
BshFI	GGCC	2 cut(s)	714, 1192
BsiHKAI	GWGCWC	3 cut(s)	276, 985, 1014
BsiSI	CCGG	2 cut(s)	35, 233
BsiWI	CGTACG	1 cut(s)	452
BslFI	GGGAC	1 cut(s)	1067
BslI	CCNNNNNNNGG	4 cut(s)	81, 577, 623, 741
BsmAI	GTCTC	3 cut(s)	461, 1074, 1087
BsmBI	CGTCTC	1 cut(s)	461
BsmFI	GGGAC	1 cut(s)	1067
BsmI	GAATGC	1 cut(s)	681
BsnI	GGCC	2 cut(s)	714, 1192
Bsp1286I	GDGCHC	3 cut(s)	276, 985, 1014
Bsp143I	GATC	3 cut(s)	428, 448, 969
BspACI	CCGC	4 cut(s)	610, 798, 884, 1047
BspANI	GGCC	2 cut(s)	714, 1192
BspCNI	CTCAG	4 cut(s)	65, 1097, 1153, 1174
BspFNI	CGCG	1 cut(s)	798
BspHI	TCATGA	1 cut(s)	1006
BspLI	GGNNCC	1 cut(s)	971
BspMAI	CTGCAG	2 cut(s)	310, 1081
BspMI	ACCTGC	2 cut(s)	121, 586
BspPI	GGATC	2 cut(s)	964, 977
BsrDI	GCAATG	1 cut(s)	323
BsrI	ACTGG	2 cut(s)	715, 735
BssECI	CCNNGG	2 cut(s)	192, 1105
BssMI	GATC	3 cut(s)	428, 448, 969
Bst2UI	CCWGG	3 cut(s)	698, 998, 1106
Bst4CI	ACNGT	4 cut(s)	193, 562, 1058, 1123
Bst6I	CTCTTC	1 cut(s)	1021
BstC8I	GCNNGC	1 cut(s)	1133
BstDEI	CTNAG	4 cut(s)	52, 1084, 1161, 1182
BstDSI	CCRYGG	1 cut(s)	192
BstF5I	GGATG	4 cut(s)	220, 224, 400, 1159
BstFNI	CGCG	1 cut(s)	798
BstH2I	RGCGCY	1 cut(s)	66
BstHHI	GCGC	2 cut(s)	65, 1244
BstKTI	GATC	3 cut(s)	431, 451, 972
BstMAI	GTCTC	3 cut(s)	461, 1074, 1087
BstMBI	GATC	3 cut(s)	428, 448, 969
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 71, 1241
BstNI	CCWGG	3 cut(s)	698, 998, 1106
BstNSI	RCATGY	2 cut(s)	358, 1141
BstPAI	GACNNNNGTC	1 cut(s)	1079
BstSCI	CCNGG	3 cut(s)	696, 996, 1104
BstSFI	CTRYAG	2 cut(s)	306, 1077
BstUI	CGCG	1 cut(s)	798
BstV1I	GCAGC	2 cut(s)	268, 292
BstX2I	RGATCY	1 cut(s)	969
BstYI	RGATCY	1 cut(s)	969
BsuRI	GGCC	2 cut(s)	714, 1192
BtgI	CCRYGG	1 cut(s)	192
BtsCI	GGATG	4 cut(s)	220, 224, 400, 1159
BtsIMutI	CAGTG	2 cut(s)	742, 1063
BveI	ACCTGC	2 cut(s)	121, 586
Cac8I	GCNNGC	1 cut(s)	1133
CaiI	CAGNNNCTG	2 cut(s)	1072, 1091
CciI	TCATGA	1 cut(s)	1006
CfoI	GCGC	2 cut(s)	65, 1244
Cfr13I	GGNCC	1 cut(s)	1191
CseI	GACGC	1 cut(s)	668
Csp6I	GTAC	2 cut(s)	453, 958
CviAII	CATG	5 cut(s)	209, 355, 506, 1007, 1138
CviQI	GTAC	2 cut(s)	453, 958
DdeI	CTNAG	4 cut(s)	52, 1084, 1161, 1182
DpnI	GATC	3 cut(s)	430, 450, 971
DpnII	GATC	3 cut(s)	428, 448, 969
EaeI	YGGCCR	1 cut(s)	712
Eam1104I	CTCTTC	1 cut(s)	1021
EarI	CTCTTC	1 cut(s)	1021
Ecl136II	GAGCTC	1 cut(s)	1012
Eco24I	GRGCYC	1 cut(s)	1014
Eco32I	GATATC	1 cut(s)	1096
Eco53kI	GAGCTC	1 cut(s)	1012
Eco57I	CTGAAG	1 cut(s)	1049
EcoICRI	GAGCTC	1 cut(s)	1012
EcoRI	GAATTC	1 cut(s)	823
EcoRII	CCWGG	3 cut(s)	696, 996, 1104
EcoRV	GATATC	1 cut(s)	1096
EcoT38I	GRGCYC	1 cut(s)	1014
Esp3I	CGTCTC	1 cut(s)	461
FaeI	CATG	5 cut(s)	212, 358, 509, 1010, 1141
FaqI	GGGAC	1 cut(s)	1067
FatI	CATG	5 cut(s)	208, 354, 505, 1006, 1137
FauI	CCCGC	1 cut(s)	1040
FauNDI	CATATG	1 cut(s)	433
FblI	GTMKAC	1 cut(s)	606
Fnu4HI	GCNGC	2 cut(s)	282, 306
FokI	GGATG	4 cut(s)	211, 227, 407, 1166
FriOI	GRGCYC	1 cut(s)	1014
Fsp4HI	GCNGC	2 cut(s)	282, 306
FspBI	CTAG	3 cut(s)	174, 377, 912
GlaI	GCGC	2 cut(s)	64, 1243
GluI	GCNGC	2 cut(s)	282, 306
GsuI	CTGGAG	4 cut(s)	534, 718, 1019, 1049
HaeII	RGCGCY	1 cut(s)	66
HaeIII	GGCC	2 cut(s)	714, 1192
HapII	CCGG	2 cut(s)	35, 233
HgaI	GACGC	1 cut(s)	668
HhaI	GCGC	2 cut(s)	65, 1244
Hin1II	CATG	5 cut(s)	212, 358, 509, 1010, 1141
Hin6I	GCGC	2 cut(s)	63, 1242
HinP1I	GCGC	2 cut(s)	63, 1242
HincII	GTYRAC	2 cut(s)	643, 934
HindII	GTYRAC	2 cut(s)	643, 934
HindIII	AAGCTT	1 cut(s)	77
HinfI	GANTC	7 cut(s)	56, 99, 478, 809, 907, 1003, 1024
HpaII	CCGG	2 cut(s)	35, 233
HphI	GGTGA	3 cut(s)	638, 757, 812
Hpy166II	GTNNAC	3 cut(s)	607, 643, 934
Hpy188I	TCNGA	2 cut(s)	55, 1074
Hpy188III	TCNNGA	5 cut(s)	392, 551, 683, 1007, 1028
Hpy8I	GTNNAC	3 cut(s)	607, 643, 934
Hpy99I	CGWCG	1 cut(s)	684
HpyAV	CCTTC	2 cut(s)	165, 1170
HpyCH4III	ACNGT	4 cut(s)	193, 562, 1058, 1123
HpyCH4IV	ACGT	5 cut(s)	470, 630, 888, 942, 951
HpyCH4V	TGCA	6 cut(s)	116, 130, 308, 595, 791, 1079
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 71, 1241
HpyF3I	CTNAG	4 cut(s)	52, 1084, 1161, 1182
HpySE526I	ACGT	5 cut(s)	470, 630, 888, 942, 951
Hsp92II	CATG	5 cut(s)	212, 358, 509, 1010, 1141
HspAI	GCGC	2 cut(s)	63, 1242
Kzo9I	GATC	3 cut(s)	428, 448, 969
LmnI	GCTCC	2 cut(s)	279, 988
Lsp1109I	GCAGC	2 cut(s)	268, 292
LweI	GCATC	4 cut(s)	125, 582, 771, 778
MaeI	CTAG	3 cut(s)	174, 377, 912
MaeII	ACGT	5 cut(s)	470, 630, 888, 942, 951
MaeIII	GTNAC	3 cut(s)	583, 626, 760
MalI	GATC	3 cut(s)	430, 450, 971
MboI	GATC	3 cut(s)	428, 448, 969
MboII	GAAGA	7 cut(s)	33, 289, 601, 679, 857, 1008, 1220
MflI	RGATCY	1 cut(s)	969
MhlI	GDGCHC	3 cut(s)	276, 985, 1014
MlsI	TGGCCA	1 cut(s)	714
MluCI	AATT	6 cut(s)	91, 254, 384, 423, 717, 823
MluNI	TGGCCA	1 cut(s)	714
MlyI	GAGTC	6 cut(s)	50, 93, 818, 901, 1012, 1018
Mox20I	TGGCCA	1 cut(s)	714
MscI	TGGCCA	1 cut(s)	714
MseI	TTAA	5 cut(s)	249, 473, 501, 510, 945
MslI	CAYNNNNRTG	1 cut(s)	213
Msp20I	TGGCCA	1 cut(s)	714
MspA1I	CMGCKG	1 cut(s)	884
MspI	CCGG	2 cut(s)	35, 233
MspR9I	CCNGG	3 cut(s)	698, 998, 1106
Mva1269I	GAATGC	1 cut(s)	681
MvaI	CCWGG	3 cut(s)	698, 998, 1106
MvnI	CGCG	1 cut(s)	798
MwoI	GCNNNNNNNGC	3 cut(s)	14, 71, 1241
NdeI	CATATG	1 cut(s)	433
NdeII	GATC	3 cut(s)	428, 448, 969
NlaIII	CATG	5 cut(s)	212, 358, 509, 1010, 1141
NlaIV	GGNNCC	1 cut(s)	971
NmuCI	GTSAC	2 cut(s)	626, 760
NspI	RCATGY	2 cut(s)	358, 1141
PagI	TCATGA	1 cut(s)	1006
PcsI	WCGNNNNNNNCGW	1 cut(s)	948
PctI	GAATGC	1 cut(s)	681
PfeI	GAWTC	1 cut(s)	478
Pfl23II	CGTACG	1 cut(s)	452
PflMI	CCANNNNNTGG	1 cut(s)	741
PfoI	TCCNGGA	2 cut(s)	696, 996
PkrI	GCNGC	2 cut(s)	283, 307
PleI	GAGTC	6 cut(s)	50, 93, 817, 901, 1011, 1018
PpsI	GAGTC	6 cut(s)	50, 93, 817, 901, 1011, 1018
PshAI	GACNNNNGTC	1 cut(s)	1079
Psp124BI	GAGCTC	1 cut(s)	1014
Psp6I	CCWGG	3 cut(s)	696, 996, 1104
PspGI	CCWGG	3 cut(s)	696, 996, 1104
PspLI	CGTACG	1 cut(s)	452
PspN4I	GGNNCC	1 cut(s)	971
PspPI	GGNCC	1 cut(s)	1191
PstI	CTGCAG	2 cut(s)	310, 1081
PstNI	CAGNNNCTG	2 cut(s)	1072, 1091
PsuI	RGATCY	1 cut(s)	969
RsaI	GTAC	2 cut(s)	454, 959
RsaNI	GTAC	2 cut(s)	453, 958
RseI	CAYNNNNRTG	1 cut(s)	213
SacI	GAGCTC	1 cut(s)	1014
SaqAI	TTAA	5 cut(s)	249, 473, 501, 510, 945
SatI	GCNGC	2 cut(s)	282, 306
Sau3AI	GATC	3 cut(s)	428, 448, 969
Sau96I	GGNCC	1 cut(s)	1191
SchI	GAGTC	6 cut(s)	50, 93, 818, 901, 1012, 1018
ScrFI	CCNGG	3 cut(s)	698, 998, 1106
SduI	GDGCHC	3 cut(s)	276, 985, 1014
SfaNI	GCATC	4 cut(s)	125, 582, 771, 778
SfcI	CTRYAG	2 cut(s)	306, 1077
SmiMI	CAYNNNNRTG	1 cut(s)	213
SmlI	CTYRAG	4 cut(s)	58, 147, 974, 1148
SmoI	CTYRAG	4 cut(s)	58, 147, 974, 1148
SpeI	ACTAGT	1 cut(s)	173
Sse9I	AATT	6 cut(s)	91, 254, 384, 423, 717, 823
SsiI	CCGC	4 cut(s)	610, 798, 884, 1047
SspMI	CTAG	3 cut(s)	174, 377, 912
SstI	GAGCTC	1 cut(s)	1014
StyD4I	CCNGG	3 cut(s)	696, 996, 1104
TaaI	ACNGT	4 cut(s)	193, 562, 1058, 1123
TaiI	ACGT	5 cut(s)	473, 633, 891, 945, 954
TaqI	TCGA	2 cut(s)	107, 1144
TasI	AATT	6 cut(s)	91, 254, 384, 423, 717, 823
TatI	WGTACW	1 cut(s)	957
TfiI	GAWTC	1 cut(s)	478
Tru1I	TTAA	5 cut(s)	249, 473, 501, 510, 945
Tru9I	TTAA	5 cut(s)	249, 473, 501, 510, 945
TscAI	CASTG	2 cut(s)	742, 1063
TseFI	GTSAC	2 cut(s)	626, 760
TseI	GCWGC	2 cut(s)	281, 305
Tsp45I	GTSAC	2 cut(s)	626, 760
TspDTI	ATGAA	6 cut(s)	213, 235, 450, 491, 767, 836
TspGWI	ACGGA	1 cut(s)	253
TspRI	CASTG	2 cut(s)	742, 1063
Van91I	CCANNNNNTGG	1 cut(s)	741
XapI	RAATTY	2 cut(s)	717, 823
XceI	RCATGY	2 cut(s)	358, 1141
XcmI	CCANNNNNNNNNTGG	1 cut(s)	298
XmiI	GTMKAC	1 cut(s)	606
XspI	CTAG	3 cut(s)	174, 377, 912

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.