MD09G1215700.v1.1

Plays an essential role in chain termination during de novo fatty acid synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005858
Physical Location & Seq
Forward (+)
21326340 .. 21327424
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1215700.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGTAGTACTTACTGGTTCTCCACCAACGTTGTTGTTGCCAACGAAACTACTGACCTTTCCGTCGAAAACCAAATCAACATTATGCTGGGAAGGAACAAACAATGGCTCACATACTAATCAAAGTAACCCTAATTCCACACCTACTGCTAAGGTTCATGCCAAGGTCTTTCCCAAAGGCAATGTTATCGTTGCCTCCACAGAAGACGATGACAAAACTGACGCACCAGCTGCCCCTACTAAGAAAATGCCACAACATCCGAACATGATATCAGCAAAGTACGATGGAGTTTATGACATGTTTCGTGGACGATTGGACGAGAGTGGTTTTGTTTTCCAAAAAAACTTTGCAATTAGATCGTATGAGTTAGGTCCTGATGGCAAAGCAACCATGACTTGCTTGATGGAGCGTTTACAGGAAACATCAGTTCAGCACTGCAGGAGTATTGGGCTCCTTGTTGGTGGTTTTGGTTCATCACTAGAGATGAGCAAAAGGGGCCAAGTGTGGGTGGTCCGGAAGTTACAGGCTATAGTGGATAAATATCCTTCTTGTGGGACATCAGGGAACAATGGTATGTTCCGGGATTGGATATTTCGTGATTACATAACTGGAGAAATATTAATGCGAGCAACCGGTAATGGTCTTTTGAGCTGCTGGCTGAGTTTTCTTGTTTTTCTCTTTTGGGCAGTGTTGAACAAAAAAAATAATAAACATATGAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko01100 ko01212 map00061 map01100 map01212
Pfam Domains
Protein Families
Acyl-ACP_TE

Protein Analysis

242

Amino Acids

26.78

Weight (kDa)

9.51

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-ACP_TE PF01643 110 - 185 7.2e-11 Acyl-ACP thioesterase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AccIII TCCGGA 1 cut(s) 513
AclI AACGTT 1 cut(s) 29
AfaI GTAC 2 cut(s) 9, 281
AfiI CCNNNNNNNGG 2 cut(s) 505, 551
AflIII ACRYGT 1 cut(s) 297
AgeI ACCGGT 1 cut(s) 632
AgsI TTSAA 1 cut(s) 694
AluBI AGCT 2 cut(s) 230, 651
AluI AGCT 2 cut(s) 230, 651
Aor13HI TCCGGA 1 cut(s) 513
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 230, 651
AseI ATTAAT 1 cut(s) 620
AsiGI ACCGGT 1 cut(s) 632
AspS9I GGNCC 3 cut(s) 371, 496, 511
AsuC2I CCSGG 1 cut(s) 581
AvaII GGWCC 2 cut(s) 371, 511
BanII GRGCYC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 217, 638
BccI CCATC 3 cut(s) 278, 371, 397
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 339, 373
BcnI CCSGG 1 cut(s) 581
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 436, 528
BisI GCNGC 2 cut(s) 231, 652
BlsI GCNGC 2 cut(s) 232, 653
BmcAI AGTACT 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 2 cut(s) 371, 511
BmgT120I GGNCC 3 cut(s) 371, 496, 511
BmiI GGNNCC 2 cut(s) 452, 497
BmrFI CCNGG 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 581
BsaBI GATNNNNATC 1 cut(s) 540
BsaJI CCNNGG 1 cut(s) 162
BsaWI WCCGGW 2 cut(s) 513, 632
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 551
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 2 cut(s) 19, 613
Bse3DI GCAATG 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 2 cut(s) 505, 551
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 19, 613
BseXI GCAGC 2 cut(s) 217, 638
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 1 cut(s) 498
BshTI ACCGGT 1 cut(s) 632
BsiSI CCGG 3 cut(s) 514, 580, 633
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 2 cut(s) 505, 551
BsmFI GGGAC 1 cut(s) 568
BsnI GGCC 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 453
Bsp13I TCCGGA 1 cut(s) 513
Bsp143I GATC 1 cut(s) 356
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 1 cut(s) 651
BspEI TCCGGA 1 cut(s) 513
BspLI GGNNCC 2 cut(s) 452, 497
BspMAI CTGCAG 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 2 cut(s) 19, 613
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 627, 656
BstDEI CTNAG 3 cut(s) 150, 240, 659
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 1 cut(s) 359
BstMBI GATC 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 230, 495
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 1 cut(s) 579
BstSFI CTRYAG 2 cut(s) 436, 528
BstV1I GCAGC 2 cut(s) 217, 638
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 2 cut(s) 433, 693
BtsIMutI CAGTG 2 cut(s) 433, 693
Cac8I GCNNGC 2 cut(s) 627, 656
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 3 cut(s) 371, 496, 511
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 8, 280
CspAI ACCGGT 1 cut(s) 632
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 4 cut(s) 158, 265, 298, 391
CviJI RGCY 7 cut(s) 108, 230, 451, 498, 527, 651, 658
CviKI_1 RGCY 7 cut(s) 108, 230, 451, 498, 527, 651, 658
CviQI GTAC 2 cut(s) 8, 280
DdeI CTNAG 3 cut(s) 150, 240, 659
DpnI GATC 1 cut(s) 358
DpnII GATC 1 cut(s) 356
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 453
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 371, 511
EcoO109I RGGNCCY 1 cut(s) 371
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 4 cut(s) 161, 268, 301, 394
FaqI GGGAC 1 cut(s) 568
FatI CATG 4 cut(s) 157, 264, 297, 390
FauNDI CATATG 1 cut(s) 714
Fnu4HI GCNGC 2 cut(s) 231, 652
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 2 cut(s) 231, 652
FspBI CTAG 1 cut(s) 479
GluI GCNGC 2 cut(s) 231, 652
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 1 cut(s) 498
HapII CCGG 3 cut(s) 514, 580, 633
HgaI GACGC 1 cut(s) 230
Hin1II CATG 4 cut(s) 161, 268, 301, 394
HpaII CCGG 3 cut(s) 514, 580, 633
Hpy166II GTNNAC 2 cut(s) 308, 413
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 3 cut(s) 374, 514, 596
Hpy8I GTNNAC 2 cut(s) 308, 413
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 86, 555
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 350, 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 495
HpyF3I CTNAG 3 cut(s) 150, 240, 659
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 4 cut(s) 161, 268, 301, 394
Kpn2I TCCGGA 1 cut(s) 513
Kzo9I GATC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 406, 456
Lsp1109I GCAGC 2 cut(s) 217, 638
MaeI CTAG 1 cut(s) 479
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 125, 519
MalI GATC 1 cut(s) 358
MboI GATC 1 cut(s) 356
MboII GAAGA 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 453
MluCI AATT 2 cut(s) 133, 351
MnlI CCTC 1 cut(s) 205
MroI TCCGGA 1 cut(s) 513
MseI TTAA 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 3 cut(s) 514, 580, 633
MspR9I CCNGG 1 cut(s) 581
MwoI GCNNNNNNNGC 2 cut(s) 230, 495
NciI CCSGG 1 cut(s) 581
NdeI CATATG 1 cut(s) 714
NdeII GATC 1 cut(s) 356
NlaIII CATG 4 cut(s) 161, 268, 301, 394
NlaIV GGNNCC 2 cut(s) 452, 497
NspI RCATGY 1 cut(s) 301
PciI ACATGT 1 cut(s) 297
PfoI TCCNGGA 1 cut(s) 579
PinAI ACCGGT 1 cut(s) 632
PkrI GCNGC 2 cut(s) 232, 653
PpuMI RGGWCCY 1 cut(s) 371
PscI ACATGT 1 cut(s) 297
PshBI ATTAAT 1 cut(s) 620
Psp1406I AACGTT 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 452, 497
PspPI GGNCC 3 cut(s) 371, 496, 511
PspPPI RGGWCCY 1 cut(s) 371
PstI CTGCAG 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 2 cut(s) 9, 281
RsaNI GTAC 2 cut(s) 8, 280
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 2 cut(s) 231, 652
Sau3AI GATC 1 cut(s) 356
Sau96I GGNCC 3 cut(s) 371, 496, 511
ScaI AGTACT 1 cut(s) 9
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 1 cut(s) 453
SetI ASST 8 cut(s) 32, 59, 145, 156, 168, 232, 373, 653
SfcI CTRYAG 2 cut(s) 436, 528
SinI GGWCC 2 cut(s) 371, 511
Sse9I AATT 2 cut(s) 133, 351
SspI AATATT 1 cut(s) 618
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 1 cut(s) 162
TaiI ACGT 1 cut(s) 32
TaqI TCGA 1 cut(s) 65
TasI AATT 2 cut(s) 133, 351
TatI WGTACW 1 cut(s) 7
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 2 cut(s) 440, 693
TseI GCWGC 2 cut(s) 230, 651
TspDTI ATGAA 2 cut(s) 146, 462
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 2 cut(s) 440, 693
VpaK11BI GGWCC 2 cut(s) 371, 511
VspI ATTAAT 1 cut(s) 620
XceI RCATGY 1 cut(s) 301
XspI CTAG 1 cut(s) 479
ZrmI AGTACT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.