FvH4_1g05750

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011073
Physical Location & Seq
Reverse (-)
3033808 .. 3035019
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05750.t1

Sequence Viewer

Length: 1212 bp
ATGGATGCTGAAGCACAGTCTGGAACTTTTTCTTCTGAGACACAATCTAATTTTCAACCAAGTGTTGCTGTGGTTATAGGTATTCTAGCCATCATGTTTGCTCTCACTTTTGTTCTTTTAGTGTATGCCAAGTTCTGCCATAGAAGAATTTCAGCGCATGGCAACAACCATCACTTGGAAGGGAGGCGTATCGGCACAAGCTCTAGATTTTCTGGTATTGACAAGACAGTGATTGAGGCACTTCCCTTTTTCAGATTCTCTACTCTTAAAGGGTCAAAGGAAGGGCTAGAATGTTCAGTCTGCCTATCAAAATTTGAAGACATTGAAGTCCTCAGATTGCTTCCCAAGTGCAAGCATGCTTTCCACATTGATTGCATAGATCACTGGCTTGAGAAGCACTCAAGCTGTCCTCTTTGCAGGCACAAGGTCAGTTCTGAGGATCTCACATTCATTGCATACTCAAACAGTATGAGAATCTTGTGGGATAGCCAATCAGAGCGGCGAGAAGACTCAAACATTGAGCTCTTTGTTCAGAGAGAGGAAGATCACCGCGGCTCCTCAAGGTTCAGCGTGGGAAGCAGCTTTCGGAAAATGGAGAAGGGTGTTGGCATAGAAGAGGAACTATTGATCCAAGAAGAAGGTGGGATTCTAAATGAGGAGGACGATGACAAGGTCCTGCACAAGCACAAGCACAAGATTGTTGTGTCCGATTTTGTGTTCCAGAACAGATGGAGCAATGTAAGCTCCTCGGACCTCATCTTCTTGAACTCGGAGATGCTTAATACAATGTCAAGCAGTAGATTCTCCTCATCTGCACGGTCTAACAATGAGCAGCTTTCGCCGAGGGAAGCAACAGGAAATGAAGAGATCATGAAGATTAGGGAGGAGATGGAGATGAAGAGATTGTTCGAGAACAAAGTCAGCAGCACAATGAACAAAAACAGTACTAGTCCTGCACGTTCAACCCTACCAACTCATGCCTCGAGTTCTATGAATCAAGGTGAGAAGAGATCAATGTCAGAAATCACTGGTCTCTCAAGATTTGGAAATTGGGGTATCAAGAACAGAACCAAAGAGCCTTCATTGCTTGATAGTAATGTGAAAGAGGAAAGAACGAGGCGGCTTTGGTTGCCGATTGCTAGAAGAACAGTCCAGTGGTTTGCCAATAGAGAGAAAAGGACTGAACAGCCTCCACAGTCTTCAAATGTGTAA

Protein Analysis

404

Amino Acids

46.09

Weight (kDa)

8.13

Isoelectric Point (pI)

76.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 97 - 140 7.4e-13 Ring finger domain
zf-RING_11 PF17123 97 - 126 3.1e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 97 - 139 3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 98 - 139 2.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 100 - 140 1.4e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 113 - 143 7.5e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 499
AccII CGCG 1 cut(s) 552
AciI CCGC 4 cut(s) 499, 550, 552, 1120
AclWI GGATC 2 cut(s) 447, 622
AcsI RAATTY 2 cut(s) 147, 311
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 946
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 6 cut(s) 56, 317, 326, 766, 963, 1203
AhlI ACTAGT 1 cut(s) 947
AloI GAACNNNNNNTCC 2 cut(s) 612, 644
AluBI AGCT 6 cut(s) 201, 405, 523, 582, 744, 835
AluI AGCT 6 cut(s) 201, 405, 523, 582, 744, 835
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 2 cut(s) 32, 1037
AlwI GGATC 2 cut(s) 447, 622
Ama87I CYCGRG 1 cut(s) 982
ApeKI GCWGC 3 cut(s) 579, 832, 924
ApoI RAATTY 2 cut(s) 147, 311
Asp700I GAANNNNTTC 2 cut(s) 28, 148
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 673, 751
AsuHPI GGTGA 2 cut(s) 539, 1013
AvaI CYCGRG 1 cut(s) 982
AvaII GGWCC 2 cut(s) 673, 751
BanII GRGCYC 1 cut(s) 525
BbsI GAAGAC 3 cut(s) 324, 513, 1191
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 591, 844, 936
BccI CCATC 4 cut(s) 98, 177, 723, 883
BcoDI GTCTC 2 cut(s) 32, 1037
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 5 cut(s) 86, 204, 287, 948, 1140
BisI GCNGC 6 cut(s) 500, 553, 580, 833, 925, 1121
BlsI GCNGC 6 cut(s) 501, 554, 581, 834, 926, 1122
BmcAI AGTACT 1 cut(s) 946
Bme18I GGWCC 2 cut(s) 673, 751
BmeT110I CYCGRG 1 cut(s) 982
BmgT120I GGNCC 2 cut(s) 673, 751
BmiI GGNNCC 1 cut(s) 556
BmsI GCATC 1 cut(s) 765
BpiI GAAGAC 3 cut(s) 324, 513, 1191
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpuEI CTTGAG 4 cut(s) 385, 410, 544, 1021
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 3 cut(s) 550, 747, 842
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 3 cut(s) 389, 1033, 1153
Bse3DI GCAATG 3 cut(s) 450, 742, 1082
BseDI CCNNGG 3 cut(s) 550, 747, 842
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMI GCAATG 3 cut(s) 450, 742, 1082
BseMII CTCAG 3 cut(s) 27, 346, 426
BseNI ACTGG 3 cut(s) 389, 1033, 1153
BseRI GAGGAG 5 cut(s) 547, 671, 736, 796, 899
BseXI GCAGC 3 cut(s) 591, 844, 936
BsgI GTGCAG 3 cut(s) 662, 798, 939
Bsh1236I CGCG 1 cut(s) 552
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 982
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 2 cut(s) 32, 1037
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 1 cut(s) 982
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
BspACI CCGC 4 cut(s) 499, 550, 552, 1120
BspCNI CTCAG 3 cut(s) 28, 345, 427
BspFNI CGCG 1 cut(s) 552
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 2 cut(s) 447, 622
BspTNI GGTCTC 1 cut(s) 1037
BsrBI CCGCTC 1 cut(s) 499
BsrDI GCAATG 3 cut(s) 450, 742, 1082
BsrI ACTGG 3 cut(s) 389, 1033, 1153
BssECI CCNNGG 3 cut(s) 550, 747, 842
BssMI GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
Bst4CI ACNGT 7 cut(s) 18, 229, 467, 819, 944, 1150, 1197
Bst6I CTCTTC 4 cut(s) 609, 858, 893, 1001
BstC8I GCNNGC 3 cut(s) 353, 357, 419
BstDEI CTNAG 3 cut(s) 36, 332, 435
BstDSI CCRYGG 1 cut(s) 550
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 6 cut(s) 382, 442, 547, 630, 870, 1013
BstMAI GTCTC 2 cut(s) 32, 1037
BstMBI GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
BstMWI GCNNNNNNNGC 6 cut(s) 394, 576, 741, 838, 1084, 1129
BstNSI RCATGY 1 cut(s) 359
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 591, 844, 936
BstV2I GAAGAC 3 cut(s) 324, 513, 1191
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 550
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 4 cut(s) 234, 382, 1026, 1160
Cac8I GCNNGC 3 cut(s) 353, 357, 419
CciI TCATGA 1 cut(s) 870
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 673, 751
Cfr42I CCGCGG 1 cut(s) 553
Csp6I GTAC 1 cut(s) 945
CspCI CAANNNNNGTGG 2 cut(s) 353, 388
CviAII CATG 5 cut(s) 94, 158, 356, 871, 977
CviQI GTAC 1 cut(s) 945
DdeI CTNAG 3 cut(s) 36, 332, 435
DpnI GATC 6 cut(s) 381, 441, 546, 629, 869, 1012
DpnII GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 4 cut(s) 609, 858, 893, 1001
EarI CTCTTC 4 cut(s) 609, 858, 893, 1001
Ecl136II GAGCTC 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 673, 751
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 982
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 525
FaeI CATG 5 cut(s) 97, 161, 359, 874, 980
FatI CATG 5 cut(s) 93, 157, 355, 870, 976
Fnu4HI GCNGC 6 cut(s) 500, 553, 580, 833, 925, 1121
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 6 cut(s) 500, 553, 580, 833, 925, 1121
FspBI CTAG 5 cut(s) 86, 204, 287, 948, 1140
GlaI GCGC 1 cut(s) 156
GluI GCNGC 6 cut(s) 500, 553, 580, 833, 925, 1121
HhaI GCGC 1 cut(s) 157
Hin1II CATG 5 cut(s) 97, 161, 359, 874, 980
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 6 cut(s) 255, 474, 509, 646, 801, 994
HphI GGTGA 2 cut(s) 539, 1013
Hpy188III TCNNGA 8 cut(s) 21, 204, 721, 763, 871, 910, 1038, 1060
HpyAV CCTTC 5 cut(s) 173, 275, 592, 632, 1089
HpyCH4III ACNGT 7 cut(s) 18, 229, 467, 819, 944, 1150, 1197
HpyCH4IV ACGT 1 cut(s) 958
HpyCH4V TGCA 7 cut(s) 351, 375, 417, 455, 679, 815, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 394, 576, 741, 838, 1084, 1129
HpyF3I CTNAG 3 cut(s) 36, 332, 435
HpySE526I ACGT 1 cut(s) 958
Hsp92II CATG 5 cut(s) 97, 161, 359, 874, 980
HspAI GCGC 1 cut(s) 155
KspI CCGCGG 1 cut(s) 553
Kzo9I GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
LmnI GCTCC 3 cut(s) 560, 732, 749
Lsp1109I GCAGC 3 cut(s) 591, 844, 936
LweI GCATC 1 cut(s) 765
MaeI CTAG 5 cut(s) 86, 204, 287, 948, 1140
MaeII ACGT 1 cut(s) 958
MalI GATC 6 cut(s) 381, 441, 546, 629, 869, 1012
MbiI CCGCTC 1 cut(s) 499
MboI GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 525
MluCI AATT 4 cut(s) 49, 147, 311, 1048
MlyI GAGTC 1 cut(s) 503
MroXI GAANNNNTTC 2 cut(s) 28, 148
MseI TTAA 2 cut(s) 267, 780
MspA1I CMGCKG 1 cut(s) 552
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 6 cut(s) 394, 576, 741, 838, 1084, 1129
NdeII GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
NlaIII CATG 5 cut(s) 97, 161, 359, 874, 980
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 867
NspI RCATGY 1 cut(s) 359
PaeI GCATGC 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 982
PagI TCATGA 1 cut(s) 870
PdmI GAANNNNTTC 2 cut(s) 28, 148
PfeI GAWTC 5 cut(s) 255, 474, 646, 801, 994
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 6 cut(s) 501, 554, 581, 834, 926, 1122
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 673
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 673
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 2 cut(s) 673, 751
PspPPI RGGWCCY 1 cut(s) 673
PspXI VCTCGAGB 1 cut(s) 982
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 1 cut(s) 946
RsaNI GTAC 1 cut(s) 945
SacI GAGCTC 1 cut(s) 525
SacII CCGCGG 1 cut(s) 553
SaqAI TTAA 2 cut(s) 267, 780
SatI GCNGC 6 cut(s) 500, 553, 580, 833, 925, 1121
Sau3AI GATC 6 cut(s) 379, 439, 544, 627, 867, 1010
Sau96I GGNCC 2 cut(s) 673, 751
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 1 cut(s) 503
SduI GDGCHC 1 cut(s) 525
SfaNI GCATC 1 cut(s) 765
Sfr274I CTCGAG 1 cut(s) 982
Sfr303I CCGCGG 1 cut(s) 553
SgrBI CCGCGG 1 cut(s) 553
SinI GGWCC 2 cut(s) 673, 751
SlaI CTCGAG 1 cut(s) 982
SmlI CTYRAG 5 cut(s) 389, 400, 559, 982, 1036
SmoI CTYRAG 5 cut(s) 389, 400, 559, 982, 1036
SpeI ACTAGT 1 cut(s) 947
SphI GCATGC 1 cut(s) 359
Sse9I AATT 4 cut(s) 49, 147, 311, 1048
SsiI CCGC 4 cut(s) 499, 550, 552, 1120
SspMI CTAG 5 cut(s) 86, 204, 287, 948, 1140
SstI GAGCTC 1 cut(s) 525
TaaI ACNGT 7 cut(s) 18, 229, 467, 819, 944, 1150, 1197
TaiI ACGT 1 cut(s) 961
TaqI TCGA 2 cut(s) 909, 983
TasI AATT 4 cut(s) 49, 147, 311, 1048
TatI WGTACW 1 cut(s) 944
TauI GCSGC 3 cut(s) 502, 555, 1123
TfiI GAWTC 5 cut(s) 255, 474, 646, 801, 994
Tru1I TTAA 2 cut(s) 267, 780
Tru9I TTAA 2 cut(s) 267, 780
TscAI CASTG 4 cut(s) 234, 389, 1033, 1160
TseI GCWGC 3 cut(s) 579, 832, 924
TspDTI ATGAA 7 cut(s) 439, 876, 887, 911, 947, 1007, 1071
TspRI CASTG 4 cut(s) 234, 389, 1033, 1160
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 673, 751
XapI RAATTY 2 cut(s) 147, 311
XbaI TCTAGA 1 cut(s) 203
XceI RCATGY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 982
XmnI GAANNNNTTC 2 cut(s) 28, 148
XspI CTAG 5 cut(s) 86, 204, 287, 948, 1140
ZrmI AGTACT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.