MD02G1058600.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011073
Physical Location & Seq
Reverse (-)
4706545 .. 4707849
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1058600.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGATTAAAATCTCGGGTTTTCAAGATTTTGATCTTCCAATGTTTCATTCATGCTTGTTTGTTTTGCTTCTCAACTGTGTGGTCTTCCATGTTGAATCACAAGCCCCAGATGTGTCTTCTGATGAAGGAAATAAATATCAATCAGGTGTTCATATGGTTATCGGTGTTCTATCTATCATGTTTTCGATAACTTTTGCGCTCCTAGTGTATGCCAAATACTGCTATAGAAGAGTTCCTGTTCGACAAAACAGTCTCCTGGAAACAGGACTTATCAGTAGTAGCTCTCGGTTCTCTGGGATTGACAAGACAGTGATTGAGGCACTTCCCTTTTTCAGATTCTCTTCTCTCAAAGGGTCAAAGGAAGGTCTCGAATGCGCAGTCTGCCTATCGAAATTTGAAGATATTGAGATTCTCAGATTGCTTCCCAAATGCAAACATGCTTTTCACATCAGCTGCATTGATCACTGGCTGGAAAAGCACTCAAGCTGTCCTCTCTGCCGGCACAGGGTTAGCTCTGAGGATCTCACATTCGATACCTTCTCGGATAGCATGAGGGTCTTGTCGAATAACCAATCAGAGAATCGACATGACTCGAGCATAGATCTCTTTGTTCAGAGAGAGGAAGATCGCCACAGTTCTTCGAGATTCAGCGTAGGACGCAGCTTTCGAAAAAGCAAAAGGGTGGTCGAGAAGGAAGATGAATTGTTAATCAAGGAAGAAGCTGCTTGTAGTGATGTGACAAAAGAGGATGACAAAATCCTGCACAAGCACAAACACAAAATTATTTTTTCTGCTCTCGAGTTTAACAACAGATGGAGCAATCTGAGTCCATCTGATCTTATGTTCTTGAATTCGGAGATGCTCAACACGGTGTCAAGCAACAGATTCTCTCCCACAAGAATAAACGATGAGCAGTTTTTGACCGCGAAAGCAACGGGAAATGAGGAGATTATGAAGATCAGGGAGGAGATGGAGATGAAAAGATTGTTTGAGAACAAAGTTAGCACAATGAACAAAAACAGTTCGGCTACAAACTCAATTGTTCCCTCTACATCAGATAGTACAGCAACTTCAGCTCATGCATCGAGTTCTACGAATCTAGGAGAGAAAAGATCAATGTCCGAAATCACTGCTCTTTCGAGATTTGGGAACTTGGGCTCCACGAACAGGACTAGAGATCCTTCACTGCTCGAAAACAATGCGAAAGAGGAAAGAATGAGGCGGCTTTGGATGCCGATTGCCCGAAGAACAGTCCAGTCGTTTGCCAATAGAGAGAAAAGGTGTCCACGGCCCTTGGATGTATGA

Protein Analysis

435

Amino Acids

49.54

Weight (kDa)

8.42

Isoelectric Point (pI)

62.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 124 - 167 1.2e-12 Ring finger domain
zf-RING_11 PF17123 124 - 153 9.2e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 124 - 166 5.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 125 - 166 4.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 128 - 167 3.8e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 249
Acc16I TGCGCA 1 cut(s) 376
AccII CGCG 1 cut(s) 926
AciI CCGC 2 cut(s) 924, 1222
AclWI GGATC 2 cut(s) 528, 1172
AcsI RAATTY 2 cut(s) 392, 850
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 1 cut(s) 1063
AfiI CCNNNNNNNGG 2 cut(s) 505, 1167
AgsI TTSAA 4 cut(s) 23, 95, 398, 850
AjnI CCWGG 1 cut(s) 255
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AloI GAACNNNNNNTCC 2 cut(s) 1142, 1174
AluBI AGCT 7 cut(s) 282, 453, 486, 513, 663, 722, 1076
AluI AGCT 7 cut(s) 282, 453, 486, 513, 663, 722, 1076
Alw26I GTCTC 2 cut(s) 257, 371
AlwI GGATC 2 cut(s) 528, 1172
Ama87I CYCGRG 3 cut(s) 13, 592, 797
AoxI GGCC 1 cut(s) 1289
ApeKI GCWGC 3 cut(s) 453, 660, 722
ApoI RAATTY 2 cut(s) 392, 850
ArsI GACNNNNNNTTYG 6 cut(s) 363, 395, 648, 669, 680, 701
AspLEI GCGC 2 cut(s) 199, 377
AspS9I GGNCC 1 cut(s) 1290
AsuII TTCGAA 1 cut(s) 667
AvaI CYCGRG 3 cut(s) 13, 592, 797
BanII GRGCYC 1 cut(s) 1160
BarI GAAGNNNNNNTAC 2 cut(s) 1054, 1086
BbsI GAAGAC 2 cut(s) 76, 108
BbvI GCAGC 3 cut(s) 440, 672, 709
BccI CCATC 3 cut(s) 807, 838, 964
BcgI CGANNNNNNTGC 4 cut(s) 1112, 1146, 1181, 1215
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 2 cut(s) 257, 371
BfaI CTAG 3 cut(s) 203, 1100, 1173
BfmI CTRYAG 1 cut(s) 223
BglII AGATCT 1 cut(s) 601
BisI GCNGC 4 cut(s) 454, 661, 723, 1223
BlsI GCNGC 4 cut(s) 455, 662, 724, 1224
Bme1390I CCNGG 1 cut(s) 257
BmeT110I CYCGRG 3 cut(s) 13, 592, 797
BmgT120I GGNCC 1 cut(s) 1290
BmiI GGNNCC 1 cut(s) 1159
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 3 cut(s) 849, 1091, 1221
BpiI GAAGAC 2 cut(s) 76, 108
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 466
BsaBI GATNNNNATC 2 cut(s) 8, 30
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 1286, 1293
BsaXI ACNNNNNCTCC 2 cut(s) 1142, 1172
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 1167
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 2 cut(s) 470, 1255
Bse8I GATNNNNATC 2 cut(s) 8, 30
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 2 cut(s) 1286, 1293
BseGI GGATG 3 cut(s) 754, 1236, 1303
BseJI GATNNNNATC 2 cut(s) 8, 30
BseLI CCNNNNNNNGG 2 cut(s) 505, 1167
BseMII CTCAG 3 cut(s) 427, 507, 815
BseNI ACTGG 2 cut(s) 470, 1255
BseRI GAGGAG 2 cut(s) 959, 980
BseXI GCAGC 3 cut(s) 440, 672, 709
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 1 cut(s) 926
BshFI GGCC 1 cut(s) 1291
BsiHKCI CYCGRG 3 cut(s) 13, 592, 797
BsiSI CCGG 1 cut(s) 499
BslI CCNNNNNNNGG 2 cut(s) 505, 1167
BsmAI GTCTC 2 cut(s) 257, 371
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 1 cut(s) 1291
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 3 cut(s) 13, 592, 797
Bsp119I TTCGAA 1 cut(s) 667
Bsp1286I GDGCHC 1 cut(s) 1160
Bsp143I GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
BspACI CCGC 2 cut(s) 924, 1222
BspANI GGCC 1 cut(s) 1291
BspCNI CTCAG 3 cut(s) 426, 508, 816
BspFNI CGCG 1 cut(s) 926
BspLI GGNNCC 1 cut(s) 1159
BspPI GGATC 2 cut(s) 528, 1172
BspT104I TTCGAA 1 cut(s) 667
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 2 cut(s) 470, 1255
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 1286, 1293
BssMI GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
BssT1I CCWWGG 1 cut(s) 1293
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 7 cut(s) 77, 251, 310, 635, 871, 1022, 1252
Bst6I CTCTTC 2 cut(s) 223, 346
BstBI TTCGAA 1 cut(s) 667
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 3 cut(s) 413, 516, 824
BstDSI CCRYGG 1 cut(s) 1286
BstF5I GGATG 3 cut(s) 754, 1236, 1303
BstFNI CGCG 1 cut(s) 926
BstHHI GCGC 2 cut(s) 199, 377
BstKTI GATC 9 cut(s) 34, 463, 523, 604, 628, 838, 960, 1115, 1180
BstMAI GTCTC 2 cut(s) 257, 371
BstMBI GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
BstMWI GCNNNNNNNGC 5 cut(s) 381, 475, 657, 1073, 1231
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 223
BstUI CGCG 1 cut(s) 926
BstV1I GCAGC 3 cut(s) 440, 672, 709
BstV2I GAAGAC 2 cut(s) 76, 108
BstX2I RGATCY 3 cut(s) 520, 601, 1177
BstYI RGATCY 3 cut(s) 520, 601, 1177
BsuRI GGCC 1 cut(s) 1291
BtgI CCRYGG 1 cut(s) 1286
BtsCI GGATG 3 cut(s) 754, 1236, 1303
BtsI GCAGTG 2 cut(s) 1128, 1184
BtsIMutI CAGTG 4 cut(s) 315, 463, 1128, 1184
Cac8I GCNNGC 1 cut(s) 500
CfoI GCGC 2 cut(s) 199, 377
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 1 cut(s) 1290
CseI GACGC 1 cut(s) 666
Csp6I GTAC 1 cut(s) 1062
CspCI CAANNNNNGTGG 1 cut(s) 1275
CviAII CATG 7 cut(s) 51, 89, 178, 437, 550, 587, 1079
CviQI GTAC 1 cut(s) 1062
DdeI CTNAG 3 cut(s) 413, 516, 824
DpnI GATC 9 cut(s) 33, 462, 522, 603, 627, 837, 959, 1114, 1179
DpnII GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
DrdI GACNNNNNNGTC 1 cut(s) 249
DseDI GACNNNNNNGTC 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 223, 346
EarI CTCTTC 2 cut(s) 223, 346
Eco130I CCWWGG 1 cut(s) 1293
Eco24I GRGCYC 1 cut(s) 1160
Eco31I GGTCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 1056
Eco88I CYCGRG 3 cut(s) 13, 592, 797
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 1293
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 1160
ErhI CCWWGG 1 cut(s) 1293
FaeI CATG 7 cut(s) 54, 92, 181, 440, 553, 590, 1082
FatI CATG 7 cut(s) 50, 88, 177, 436, 549, 586, 1078
FauNDI CATATG 1 cut(s) 153
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 4 cut(s) 454, 661, 723, 1223
FokI GGATG 2 cut(s) 761, 1243
FriOI GRGCYC 1 cut(s) 1160
Fsp4HI GCNGC 4 cut(s) 454, 661, 723, 1223
FspBI CTAG 3 cut(s) 203, 1100, 1173
FspI TGCGCA 1 cut(s) 376
GlaI GCGC 2 cut(s) 198, 376
GluI GCNGC 4 cut(s) 454, 661, 723, 1223
HaeIII GGCC 1 cut(s) 1291
HapII CCGG 1 cut(s) 499
HgaI GACGC 1 cut(s) 666
HhaI GCGC 2 cut(s) 199, 377
Hin1II CATG 7 cut(s) 54, 92, 181, 440, 553, 590, 1082
Hin6I GCGC 2 cut(s) 197, 375
HinP1I GCGC 2 cut(s) 197, 375
HinfI GANTC 9 cut(s) 95, 336, 409, 580, 590, 645, 826, 885, 1096
HpaII CCGG 1 cut(s) 499
Hpy166II GTNNAC 1 cut(s) 1286
Hpy188III TCNNGA 7 cut(s) 23, 368, 642, 688, 797, 847, 1140
Hpy8I GTNNAC 1 cut(s) 1286
HpyAV CCTTC 5 cut(s) 119, 356, 547, 685, 1191
HpyCH4III ACNGT 7 cut(s) 77, 251, 310, 635, 871, 1022, 1252
HpyCH4V TGCA 4 cut(s) 432, 456, 763, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 381, 475, 657, 1073, 1231
HpyF3I CTNAG 3 cut(s) 413, 516, 824
Hsp92II CATG 7 cut(s) 54, 92, 181, 440, 553, 590, 1082
HspAI GCGC 2 cut(s) 197, 375
KroI GCCGGC 1 cut(s) 498
KroNI GCCGGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
LmnI GCTCC 3 cut(s) 204, 816, 1163
Lsp1109I GCAGC 3 cut(s) 440, 672, 709
LweI GCATC 3 cut(s) 849, 1091, 1221
MaeI CTAG 3 cut(s) 203, 1100, 1173
MaeIII GTNAC 1 cut(s) 736
MalI GATC 9 cut(s) 33, 462, 522, 603, 627, 837, 959, 1114, 1179
MboI GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
MfeI CAATTG 1 cut(s) 1038
MflI RGATCY 3 cut(s) 520, 601, 1177
MhlI GDGCHC 1 cut(s) 1160
MluCI AATT 5 cut(s) 392, 701, 780, 850, 1038
MlyI GAGTC 2 cut(s) 584, 835
Mph1103I ATGCAT 1 cut(s) 1084
MroNI GCCGGC 1 cut(s) 498
MseI TTAA 3 cut(s) 6, 707, 804
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 1 cut(s) 499
MspR9I CCNGG 1 cut(s) 257
MunI CAATTG 1 cut(s) 1038
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 5 cut(s) 381, 475, 657, 1073, 1231
NaeI GCCGGC 1 cut(s) 500
NdeI CATATG 1 cut(s) 153
NdeII GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
NgoMIV GCCGGC 1 cut(s) 498
NlaIII CATG 7 cut(s) 54, 92, 181, 440, 553, 590, 1082
NlaIV GGNNCC 1 cut(s) 1159
NmuCI GTSAC 1 cut(s) 736
NsbI TGCGCA 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 440
NspV TTCGAA 1 cut(s) 667
PaeR7I CTCGAG 2 cut(s) 592, 797
PcsI WCGNNNNNNNCGW 2 cut(s) 664, 1091
PctI GAATGC 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 500
PfeI GAWTC 7 cut(s) 95, 336, 409, 580, 645, 885, 1096
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 4 cut(s) 455, 662, 724, 1224
PleI GAGTC 2 cut(s) 584, 834
PpsI GAGTC 2 cut(s) 584, 834
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 1159
PspPI GGNCC 1 cut(s) 1290
PspXI VCTCGAGB 1 cut(s) 592
PsuI RGATCY 3 cut(s) 520, 601, 1177
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 1 cut(s) 1063
RsaNI GTAC 1 cut(s) 1062
SaqAI TTAA 3 cut(s) 6, 707, 804
SatI GCNGC 4 cut(s) 454, 661, 723, 1223
Sau3AI GATC 9 cut(s) 31, 460, 520, 601, 625, 835, 957, 1112, 1177
Sau96I GGNCC 1 cut(s) 1290
SchI GAGTC 2 cut(s) 584, 835
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 1 cut(s) 1160
SfaNI GCATC 3 cut(s) 849, 1091, 1221
SfcI CTRYAG 1 cut(s) 223
Sfr274I CTCGAG 2 cut(s) 592, 797
SfuI TTCGAA 1 cut(s) 667
SlaI CTCGAG 2 cut(s) 592, 797
SmlI CTYRAG 3 cut(s) 481, 592, 797
SmoI CTYRAG 3 cut(s) 481, 592, 797
Sse9I AATT 5 cut(s) 392, 701, 780, 850, 1038
SsiI CCGC 2 cut(s) 924, 1222
SspMI CTAG 3 cut(s) 203, 1100, 1173
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 1293
TaaI ACNGT 7 cut(s) 77, 251, 310, 635, 871, 1022, 1252
TasI AATT 5 cut(s) 392, 701, 780, 850, 1038
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 1225
TfiI GAWTC 7 cut(s) 95, 336, 409, 580, 645, 885, 1096
Tru1I TTAA 3 cut(s) 6, 707, 804
Tru9I TTAA 3 cut(s) 6, 707, 804
TscAI CASTG 4 cut(s) 315, 470, 1135, 1191
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 3 cut(s) 453, 660, 722
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 8 cut(s) 35, 39, 138, 140, 714, 968, 992, 1025
TspRI CASTG 4 cut(s) 315, 470, 1135, 1191
XapI RAATTY 2 cut(s) 392, 850
XceI RCATGY 1 cut(s) 440
XhoI CTCGAG 2 cut(s) 592, 797
XspI CTAG 3 cut(s) 203, 1100, 1173
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.