Rh2AG062400

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011073
Physical Location & Seq
Reverse (-)
5109647 .. 5110870
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG062400.1

Sequence Viewer

Length: 1224 bp
ATGGATGCTGAAGCACAATCTGTAGGCGGTTATGATTCTGAAACAGATCAATCTAATTTTCAACCAAGTGTTGCTGTGGTTATAGGTATTTTAGCCATCATGTTTGCTCTGACTTTTGTTCTCTTACTGTATGCCAAGTTCTGCCATAGAAGAGTTTCTGTTCATGGCAACCATCACTTGGAAGGGGGGCGTATCGGCACAAGCTCTCGGTTTTCTGGTATTGACAAGACAGTGATTGAGGCACTTCCCTTTTTCAGATTCTCTAATCTTAAAGGGTCAAAGGAAGGGCTAGAATGTGCAGTCTGCCTATCAAAATTTGAAGACATCGAAGTCCTCAGATTGCTTCCCAAGTGCAAGCATGCTTTCCACATTGATTGCATCGATCACTGGCTTGAGAAGCACTCCAGCTGTCCTCTTTGCAGGCACAAGGTCAGTTCTGAGGACCTCACGTTCATTGCATACTCCAACAGTATGAGAGTCTTGTGGGATAGCCAATCAGAGCGGCGAGAAGACTCAAACATTGAGCTCTTTGTTCAGAGAGAGGAAGACCACCGCGGCTCTTCAAGGTTCAGTGTGGGAAGTAGCTTTCGGAAAATGGAAAAGGGCGTTGATACAGGAGAGGAACTGTTGATCCAAGAAGAAGCTGGGATTCTCAAGGAGGAGGAGGAGGAGGATGACAAGGTCCTGCACAAACACAAGCACAAAATTGTTGTGTCAGATTTTGTGTTCCAGAACAGATGGAGTAATCTGAGTTCCTCGGACCTCATTTTCTTGAACTCGGAGATGCTTAATACAATGTCAAGCAGTAGATTCTCCACATCTGCTCGTTTAAACAATGAGCAGTTTTCGCCGATGGAAGCGTCGACAGGAAATGATGAGATTATGAAGATTAGGGAGGAGATGGAGATGAAGAGATTGTTCGAAAACAAAGTCAGCAGCACAATGAACAAAAACAGTACTACTTTAGCAGCTTCAACCCTACCAACTCATGCCTCGAGTTCTATGAACCAAGGTGAGAAGAGATCAATGTCAGAAATCACTGCTCTTTCGAGATTTGGAAACTGGGGTATCAAGAACAGAACCAAAGAGCCTTCATTGCTTGGTAACAATGTGAAAGAGGAAAGAATGAGGCGGCTTTGGTTGCCGATTGCCAGAAGAACAGTCCAATGGTTTGCCAATAGAGAGAAAAGGTCTGAGCAGCCTCAACATTCTTCAAATGTGTAA

Protein Analysis

407

Amino Acids

46.5

Weight (kDa)

6.59

Isoelectric Point (pI)

67.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 98 - 141 6.6e-13 Ring finger domain
zf-RING_11 PF17123 98 - 127 4e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 98 - 140 1.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 99 - 140 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 102 - 141 2.7e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 114 - 144 7.2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccB7I CCANNNNNTGG 1 cut(s) 178
AccBSI CCGCTC 1 cut(s) 502
AccI GTMKAC 1 cut(s) 863
AccII CGCG 1 cut(s) 555
AciI CCGC 5 cut(s) 27, 502, 553, 555, 1132
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 958
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 6 cut(s) 62, 320, 564, 775, 975, 1215
AloI GAACNNNNNNTCC 4 cut(s) 434, 466, 615, 647
AluBI AGCT 6 cut(s) 204, 408, 526, 585, 644, 971
AluI AGCT 6 cut(s) 204, 408, 526, 585, 644, 971
Alw21I GWGCWC 1 cut(s) 528
AlwI GGATC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 994
ApeKI GCWGC 3 cut(s) 936, 968, 1198
ApoI RAATTY 1 cut(s) 314
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
Asp700I GAANNNNTTC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 442, 682, 760
AsuHPI GGTGA 1 cut(s) 1025
AsuII TTCGAA 1 cut(s) 921
AvaI CYCGRG 1 cut(s) 994
AvaII GGWCC 3 cut(s) 442, 682, 760
BaeI ACNNNNGTAYC 2 cut(s) 1051, 1084
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 3 cut(s) 327, 516, 552
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 948, 980, 1210
BccI CCATC 5 cut(s) 104, 180, 732, 847, 895
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 6 cut(s) 503, 556, 937, 969, 1133, 1199
BlsI GCNGC 6 cut(s) 504, 557, 938, 970, 1134, 1200
BmcAI AGTACT 1 cut(s) 958
Bme18I GGWCC 3 cut(s) 442, 682, 760
BmeT110I CYCGRG 1 cut(s) 994
BmgT120I GGNCC 3 cut(s) 442, 682, 760
BmrI ACTGGG 1 cut(s) 1072
BmsI GCATC 2 cut(s) 387, 774
BmuI ACTGGG 1 cut(s) 1072
BpiI GAAGAC 3 cut(s) 327, 516, 552
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 921
BpuEI CTTGAG 2 cut(s) 413, 638
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 553, 756, 1009
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 392, 1067
Bse3DI GCAATG 2 cut(s) 453, 1094
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 3 cut(s) 553, 756, 1009
BseGI GGATG 2 cut(s) 10, 679
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 453, 1094
BseMII CTCAG 4 cut(s) 349, 429, 740, 1185
BseNI ACTGG 2 cut(s) 392, 1067
BseRI GAGGAG 5 cut(s) 674, 677, 680, 683, 911
BseXI GCAGC 3 cut(s) 948, 980, 1210
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 2 cut(s) 318, 671
Bsh1236I CGCG 1 cut(s) 555
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 994
BslI CCNNNNNNNGG 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 994
Bsp119I TTCGAA 1 cut(s) 921
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 4 cut(s) 46, 382, 630, 1022
BspACI CCGC 5 cut(s) 27, 502, 553, 555, 1132
BspCNI CTCAG 4 cut(s) 348, 430, 741, 1186
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 1 cut(s) 555
BspPI GGATC 1 cut(s) 625
BspQI GCTCTTC 1 cut(s) 565
BspT104I TTCGAA 1 cut(s) 921
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 2 cut(s) 453, 1094
BsrI ACTGG 2 cut(s) 392, 1067
BssECI CCNNGG 3 cut(s) 553, 756, 1009
BssMI GATC 4 cut(s) 46, 382, 630, 1022
BssT1I CCWWGG 1 cut(s) 1009
Bst4CI ACNGT 6 cut(s) 129, 232, 470, 627, 956, 1162
Bst6I CTCTTC 4 cut(s) 145, 565, 905, 1013
BstBI TTCGAA 1 cut(s) 921
BstC8I GCNNGC 3 cut(s) 356, 360, 422
BstDEI CTNAG 4 cut(s) 335, 438, 749, 1194
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 10, 679
BstFNI CGCG 1 cut(s) 555
BstKTI GATC 4 cut(s) 49, 385, 633, 1025
BstMBI GATC 4 cut(s) 46, 382, 630, 1022
BstMWI GCNNNNNNNGC 4 cut(s) 397, 847, 1096, 1141
BstNSI RCATGY 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 3 cut(s) 948, 980, 1210
BstV2I GAAGAC 3 cut(s) 327, 516, 552
Bsu15I ATCGAT 1 cut(s) 381
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 2 cut(s) 10, 679
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 4 cut(s) 237, 385, 577, 1038
Cac8I GCNNGC 3 cut(s) 356, 360, 422
Cfr13I GGNCC 3 cut(s) 442, 682, 760
Cfr42I CCGCGG 1 cut(s) 556
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 849
Csp6I GTAC 1 cut(s) 957
CspCI CAANNNNNGTGG 2 cut(s) 356, 391
CviAII CATG 4 cut(s) 100, 164, 359, 989
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 4 cut(s) 335, 438, 749, 1194
DpnI GATC 4 cut(s) 48, 384, 632, 1024
DpnII GATC 4 cut(s) 46, 382, 630, 1022
DraI TTTAAA 1 cut(s) 831
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
Eam1104I CTCTTC 4 cut(s) 145, 565, 905, 1013
EarI CTCTTC 4 cut(s) 145, 565, 905, 1013
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 1009
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 3 cut(s) 442, 682, 760
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 994
EcoICRI GAGCTC 1 cut(s) 526
EcoO109I RGGNCCY 2 cut(s) 442, 682
EcoT14I CCWWGG 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 1009
FaeI CATG 4 cut(s) 103, 167, 362, 992
FatI CATG 4 cut(s) 99, 163, 358, 988
FblI GTMKAC 1 cut(s) 863
Fnu4HI GCNGC 6 cut(s) 503, 556, 937, 969, 1133, 1199
FokI GGATG 2 cut(s) 17, 686
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 6 cut(s) 503, 556, 937, 969, 1133, 1199
FspBI CTAG 1 cut(s) 290
GluI GCNGC 6 cut(s) 503, 556, 937, 969, 1133, 1199
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 1 cut(s) 388
HgaI GACGC 1 cut(s) 849
Hin1II CATG 4 cut(s) 103, 167, 362, 992
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HinfI GANTC 6 cut(s) 35, 258, 477, 512, 649, 810
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 1 cut(s) 864
Hpy188III TCNNGA 4 cut(s) 730, 772, 1050, 1072
Hpy8I GTNNAC 1 cut(s) 864
Hpy99I CGWCG 1 cut(s) 865
HpyAV CCTTC 3 cut(s) 176, 278, 1101
HpyCH4III ACNGT 6 cut(s) 129, 232, 470, 627, 956, 1162
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 299, 354, 378, 420, 458, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 397, 847, 1096, 1141
HpyF3I CTNAG 4 cut(s) 335, 438, 749, 1194
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 4 cut(s) 103, 167, 362, 992
KspI CCGCGG 1 cut(s) 556
Kzo9I GATC 4 cut(s) 46, 382, 630, 1022
LguI GCTCTTC 1 cut(s) 565
Lsp1109I GCAGC 3 cut(s) 948, 980, 1210
LweI GCATC 2 cut(s) 387, 774
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 1 cut(s) 1103
MalI GATC 4 cut(s) 48, 384, 632, 1024
MbiI CCGCTC 1 cut(s) 502
MboI GATC 4 cut(s) 46, 382, 630, 1022
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 3 cut(s) 55, 314, 705
MlyI GAGTC 2 cut(s) 486, 506
MmeI TCCRAC 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 154
MseI TTAA 3 cut(s) 270, 789, 830
MspA1I CMGCKG 2 cut(s) 408, 555
MssI GTTTAAAC 1 cut(s) 831
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 4 cut(s) 397, 847, 1096, 1141
NdeII GATC 4 cut(s) 46, 382, 630, 1022
NlaIII CATG 4 cut(s) 103, 167, 362, 992
NspI RCATGY 1 cut(s) 362
NspV TTCGAA 1 cut(s) 921
PaeI GCATGC 1 cut(s) 362
PaeR7I CTCGAG 1 cut(s) 994
PciSI GCTCTTC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 154
PfeI GAWTC 4 cut(s) 35, 258, 649, 810
PflFI GACNNNGTC 1 cut(s) 680
PflMI CCANNNNNTGG 1 cut(s) 178
PkrI GCNGC 6 cut(s) 504, 557, 938, 970, 1134, 1200
PleI GAGTC 2 cut(s) 485, 506
PmeI GTTTAAAC 1 cut(s) 831
PpsI GAGTC 2 cut(s) 485, 506
PpuMI RGGWCCY 2 cut(s) 442, 682
Psp124BI GAGCTC 1 cut(s) 528
Psp5II RGGWCCY 2 cut(s) 442, 682
PspFI CCCAGC 1 cut(s) 644
PspPI GGNCC 3 cut(s) 442, 682, 760
PspPPI RGGWCCY 2 cut(s) 442, 682
PspXI VCTCGAGB 1 cut(s) 994
PsrI GAACNNNNNNTAC 4 cut(s) 122, 154, 736, 768
PsyI GACNNNGTC 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 408
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
SacI GAGCTC 1 cut(s) 528
SacII CCGCGG 1 cut(s) 556
SalI GTCGAC 1 cut(s) 862
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 3 cut(s) 270, 789, 830
SatI GCNGC 6 cut(s) 503, 556, 937, 969, 1133, 1199
Sau3AI GATC 4 cut(s) 46, 382, 630, 1022
Sau96I GGNCC 3 cut(s) 442, 682, 760
ScaI AGTACT 1 cut(s) 958
SchI GAGTC 2 cut(s) 486, 506
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 387, 774
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 994
Sfr303I CCGCGG 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 921
SgrBI CCGCGG 1 cut(s) 556
SinI GGWCC 3 cut(s) 442, 682, 760
SlaI CTCGAG 1 cut(s) 994
SmlI CTYRAG 3 cut(s) 392, 653, 994
SmoI CTYRAG 3 cut(s) 392, 653, 994
SphI GCATGC 1 cut(s) 362
Sse9I AATT 3 cut(s) 55, 314, 705
SsiI CCGC 5 cut(s) 27, 502, 553, 555, 1132
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 528
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 6 cut(s) 129, 232, 470, 627, 956, 1162
TaiI ACGT 1 cut(s) 452
TaqI TCGA 6 cut(s) 327, 381, 863, 921, 995, 1049
TasI AATT 3 cut(s) 55, 314, 705
TatI WGTACW 1 cut(s) 956
TauI GCSGC 3 cut(s) 505, 558, 1135
TfiI GAWTC 4 cut(s) 35, 258, 649, 810
Tru1I TTAA 3 cut(s) 270, 789, 830
Tru9I TTAA 3 cut(s) 270, 789, 830
TscAI CASTG 4 cut(s) 237, 392, 577, 1045
TseI GCWGC 3 cut(s) 936, 968, 1198
TspDTI ATGAA 7 cut(s) 152, 442, 899, 923, 959, 1019, 1083
TspRI CASTG 4 cut(s) 237, 392, 577, 1045
Tth111I GACNNNGTC 1 cut(s) 680
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 3 cut(s) 442, 682, 760
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XhoI CTCGAG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 863
XmnI GAANNNNTTC 1 cut(s) 154
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.