RLG00000016220

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011073
Physical Location & Seq
Reverse (-)
4822971 .. 4824188
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016220

Sequence Viewer

Length: 1218 bp
ATGGATGCTGAAGCACAATCTGTAGGCGGTTATGATTCTGAAACAGATCAATCTAATTTTCAACCAAGTGTTGCTGTGGTTATAGGTATTTTAGCCCTCATGTTTGCTCTGACTTTTGTTCTCTTAGTGTATGCCAAGTTCTGCCATAGAAGAGTTTCTGTTCATGGCAACCATCACTTGGAAGGGGGGCGTATCGGCACAAGCTCTCGGTTTTCTGGTATTGACAAGACAGTGATTGAGGCACTTCCCTTTTTCAGATTCTCTAATCTTAAAGGGTCAAAGGAAGGGCTAGAATGTGCAGTCTGCCTATCAAAATTTGAAGACATCGAAGTCCTCAGATTGCTTCCCAAGTGCAAGCATGCTTTCCACATTGATTGCATCGATTACTGGCTTGAGAAGCACTCCAGCTGTCCACTTTGCAGGCACAAGGTCAGTTCTGAGGACCTCACGTTCATTGCATACTCCAACAGTATGAGAGTCTTGTGGGATAGCCAATCAGAGCGGCGAGAAGACTCAAACATTGAGCTCTTTGTTCAGAGAGAGGAAGACCACCGCGGCTCTTCAAGGTTCAGTGTGGGAAGTAGCTTTCGGAAAATGGAAAAGGGCGTTGATAAAGGAGAGGAACTGTTGATCCAAGAAGAAGCTCGGATTCTCAAGGAGGATGATGATGATGACAAGGTCCTGCACAAACACAAGCACAAAATTGTTGTGTCTGATTTTGTGTTCCAGAACAGATGGAGTAATCTGAGTTCCTCGGACCTCATTTTCTTGAACTCGGAGATGCTTAATACAATGTCGAGCAGTAGATTCTCCACATCAGCTCGTTTAAACAATGAGCAGTTTTCGCCGAGGGAAGCGACAGGAAATGATGAGATTATGAAGATTAGGGAGGAGATGGAGATGAAGAGATTGTTCGAAAACAAAGTCAGCAGCACAATGAACAAAAACAGTACTACTTTTGCAGCTTCAACCCTACCAACTCATGCCTCGAGTTCTATGAATCAAGGTGAGAAGAGATCAATGTCAGAAATCACTGCTCTTTCGAGATTTGGAAACTGGGGTATCAAGAACAAAACCAAAGAGCCTTCATTGCTTGGTAACAATGTGAAAGAGGAAAGAATGAGGCGGCTTTGGTTGCCGATTGCCAGAAGAACAGTCCAGTGGTTTGCCAATAGAGAGAAAAGGTCTGAACAGCCTCAACATTCTTCAAATGTGTAA

Protein Analysis

406

Amino Acids

46.41

Weight (kDa)

7.68

Isoelectric Point (pI)

65.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 98 - 141 1.2e-12 Ring finger domain
zf-RING_11 PF17123 98 - 127 4e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 98 - 140 1.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 99 - 140 5.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 102 - 141 1.5e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 114 - 144 8.7e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccB7I CCANNNNNTGG 1 cut(s) 178
AccBSI CCGCTC 1 cut(s) 502
AccII CGCG 1 cut(s) 555
AciI CCGC 5 cut(s) 27, 502, 553, 555, 1126
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 952
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 6 cut(s) 62, 320, 564, 772, 969, 1209
AloI GAACNNNNNNTCC 4 cut(s) 434, 466, 615, 647
AluBI AGCT 7 cut(s) 204, 408, 526, 585, 644, 821, 965
AluI AGCT 7 cut(s) 204, 408, 526, 585, 644, 821, 965
Alw21I GWGCWC 1 cut(s) 528
AlwI GGATC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 988
ApeKI GCWGC 2 cut(s) 930, 962
ApoI RAATTY 1 cut(s) 314
ArsI GACNNNNNNTTYG 2 cut(s) 909, 941
Asp700I GAANNNNTTC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 442, 679, 757
AsuHPI GGTGA 1 cut(s) 1019
AsuII TTCGAA 1 cut(s) 915
AvaI CYCGRG 1 cut(s) 988
AvaII GGWCC 3 cut(s) 442, 679, 757
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BanII GRGCYC 1 cut(s) 528
BbsI GAAGAC 3 cut(s) 327, 516, 552
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 942, 974
BccI CCATC 3 cut(s) 180, 729, 889
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 5 cut(s) 503, 556, 931, 963, 1127
BlsI GCNGC 5 cut(s) 504, 557, 932, 964, 1128
BmcAI AGTACT 1 cut(s) 952
Bme18I GGWCC 3 cut(s) 442, 679, 757
BmeT110I CYCGRG 1 cut(s) 988
BmgT120I GGNCC 3 cut(s) 442, 679, 757
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 2 cut(s) 387, 771
BmuI ACTGGG 1 cut(s) 1066
BpiI GAAGAC 3 cut(s) 327, 516, 552
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 915
BpuEI CTTGAG 2 cut(s) 413, 638
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 553, 753, 848
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse1I ACTGG 3 cut(s) 392, 1061, 1159
Bse3DI GCAATG 2 cut(s) 453, 1088
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 3 cut(s) 553, 753, 848
BseGI GGATG 2 cut(s) 10, 667
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 453, 1088
BseMII CTCAG 3 cut(s) 349, 429, 737
BseNI ACTGG 3 cut(s) 392, 1061, 1159
BseRI GAGGAG 1 cut(s) 905
BseXI GCAGC 2 cut(s) 942, 974
BsgI GTGCAG 2 cut(s) 318, 668
Bsh1236I CGCG 1 cut(s) 555
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 988
BslI CCNNNNNNNGG 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 988
Bsp119I TTCGAA 1 cut(s) 915
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 3 cut(s) 46, 630, 1016
BspACI CCGC 5 cut(s) 27, 502, 553, 555, 1126
BspCNI CTCAG 3 cut(s) 348, 430, 738
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 1 cut(s) 555
BspPI GGATC 1 cut(s) 625
BspQI GCTCTTC 1 cut(s) 565
BspT104I TTCGAA 1 cut(s) 915
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 2 cut(s) 453, 1088
BsrI ACTGG 3 cut(s) 392, 1061, 1159
BssECI CCNNGG 3 cut(s) 553, 753, 848
BssMI GATC 3 cut(s) 46, 630, 1016
Bst4CI ACNGT 5 cut(s) 232, 470, 627, 950, 1156
Bst6I CTCTTC 4 cut(s) 145, 565, 899, 1007
BstBI TTCGAA 1 cut(s) 915
BstC8I GCNNGC 3 cut(s) 356, 360, 422
BstDEI CTNAG 4 cut(s) 124, 335, 438, 746
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 10, 667
BstFNI CGCG 1 cut(s) 555
BstKTI GATC 3 cut(s) 49, 633, 1019
BstMBI GATC 3 cut(s) 46, 630, 1016
BstMWI GCNNNNNNNGC 4 cut(s) 397, 844, 1090, 1135
BstNSI RCATGY 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 2 cut(s) 942, 974
BstV2I GAAGAC 3 cut(s) 327, 516, 552
Bsu15I ATCGAT 1 cut(s) 381
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 2 cut(s) 10, 667
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 4 cut(s) 237, 577, 1032, 1166
Cac8I GCNNGC 3 cut(s) 356, 360, 422
Cfr13I GGNCC 3 cut(s) 442, 679, 757
Cfr42I CCGCGG 1 cut(s) 556
ClaI ATCGAT 1 cut(s) 381
Csp6I GTAC 1 cut(s) 951
CspCI CAANNNNNGTGG 2 cut(s) 356, 391
CviAII CATG 4 cut(s) 100, 164, 359, 983
CviQI GTAC 1 cut(s) 951
DdeI CTNAG 4 cut(s) 124, 335, 438, 746
DpnI GATC 3 cut(s) 48, 632, 1018
DpnII GATC 3 cut(s) 46, 630, 1016
DraI TTTAAA 1 cut(s) 828
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
Eam1104I CTCTTC 4 cut(s) 145, 565, 899, 1007
EarI CTCTTC 4 cut(s) 145, 565, 899, 1007
Ecl136II GAGCTC 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 3 cut(s) 442, 679, 757
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 988
EcoICRI GAGCTC 1 cut(s) 526
EcoO109I RGGNCCY 2 cut(s) 442, 679
EcoT38I GRGCYC 1 cut(s) 528
FaeI CATG 4 cut(s) 103, 167, 362, 986
FatI CATG 4 cut(s) 99, 163, 358, 982
Fnu4HI GCNGC 5 cut(s) 503, 556, 931, 963, 1127
FokI GGATG 2 cut(s) 17, 674
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 5 cut(s) 503, 556, 931, 963, 1127
FspBI CTAG 1 cut(s) 290
GluI GCNGC 5 cut(s) 503, 556, 931, 963, 1127
GsuI CTGGAG 1 cut(s) 388
Hin1II CATG 4 cut(s) 103, 167, 362, 986
HinfI GANTC 7 cut(s) 35, 258, 477, 512, 649, 807, 1000
HphI GGTGA 1 cut(s) 1019
Hpy166II GTNNAC 1 cut(s) 413
Hpy188III TCNNGA 4 cut(s) 727, 769, 1044, 1066
Hpy8I GTNNAC 1 cut(s) 413
HpyAV CCTTC 3 cut(s) 176, 278, 1095
HpyCH4III ACNGT 5 cut(s) 232, 470, 627, 950, 1156
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 7 cut(s) 299, 354, 378, 420, 458, 685, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 397, 844, 1090, 1135
HpyF3I CTNAG 4 cut(s) 124, 335, 438, 746
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 4 cut(s) 103, 167, 362, 986
KspI CCGCGG 1 cut(s) 556
Kzo9I GATC 3 cut(s) 46, 630, 1016
LguI GCTCTTC 1 cut(s) 565
Lsp1109I GCAGC 2 cut(s) 942, 974
LweI GCATC 2 cut(s) 387, 771
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 1 cut(s) 1097
MalI GATC 3 cut(s) 48, 632, 1018
MbiI CCGCTC 1 cut(s) 502
MboI GATC 3 cut(s) 46, 630, 1016
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 3 cut(s) 55, 314, 702
MlyI GAGTC 2 cut(s) 486, 506
MmeI TCCRAC 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 154
MseI TTAA 3 cut(s) 270, 786, 827
MspA1I CMGCKG 2 cut(s) 408, 555
MssI GTTTAAAC 1 cut(s) 828
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 4 cut(s) 397, 844, 1090, 1135
NdeII GATC 3 cut(s) 46, 630, 1016
NlaIII CATG 4 cut(s) 103, 167, 362, 986
NmeAIII GCCGAG 1 cut(s) 873
NspI RCATGY 1 cut(s) 362
NspV TTCGAA 1 cut(s) 915
PaeI GCATGC 1 cut(s) 362
PaeR7I CTCGAG 1 cut(s) 988
PciSI GCTCTTC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 154
PfeI GAWTC 5 cut(s) 35, 258, 649, 807, 1000
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 1 cut(s) 178
PkrI GCNGC 5 cut(s) 504, 557, 932, 964, 1128
PleI GAGTC 2 cut(s) 485, 506
PmeI GTTTAAAC 1 cut(s) 828
PpsI GAGTC 2 cut(s) 485, 506
PpuMI RGGWCCY 2 cut(s) 442, 679
Psp124BI GAGCTC 1 cut(s) 528
Psp5II RGGWCCY 2 cut(s) 442, 679
PspPI GGNCC 3 cut(s) 442, 679, 757
PspPPI RGGWCCY 2 cut(s) 442, 679
PspXI VCTCGAGB 1 cut(s) 988
PsrI GAACNNNNNNTAC 4 cut(s) 122, 154, 733, 765
PsyI GACNNNGTC 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 408
RsaI GTAC 1 cut(s) 952
RsaNI GTAC 1 cut(s) 951
SacI GAGCTC 1 cut(s) 528
SacII CCGCGG 1 cut(s) 556
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 3 cut(s) 270, 786, 827
SatI GCNGC 5 cut(s) 503, 556, 931, 963, 1127
Sau3AI GATC 3 cut(s) 46, 630, 1016
Sau96I GGNCC 3 cut(s) 442, 679, 757
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 2 cut(s) 486, 506
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 387, 771
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 988
Sfr303I CCGCGG 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 915
SgrBI CCGCGG 1 cut(s) 556
SinI GGWCC 3 cut(s) 442, 679, 757
SlaI CTCGAG 1 cut(s) 988
SmlI CTYRAG 3 cut(s) 392, 653, 988
SmoI CTYRAG 3 cut(s) 392, 653, 988
SphI GCATGC 1 cut(s) 362
Sse9I AATT 3 cut(s) 55, 314, 702
SsiI CCGC 5 cut(s) 27, 502, 553, 555, 1126
SspMI CTAG 1 cut(s) 290
SstI GAGCTC 1 cut(s) 528
TaaI ACNGT 5 cut(s) 232, 470, 627, 950, 1156
TaiI ACGT 1 cut(s) 452
TaqI TCGA 6 cut(s) 327, 381, 797, 915, 989, 1043
TasI AATT 3 cut(s) 55, 314, 702
TatI WGTACW 1 cut(s) 950
TauI GCSGC 3 cut(s) 505, 558, 1129
TfiI GAWTC 5 cut(s) 35, 258, 649, 807, 1000
Tru1I TTAA 3 cut(s) 270, 786, 827
Tru9I TTAA 3 cut(s) 270, 786, 827
TscAI CASTG 4 cut(s) 237, 577, 1039, 1166
TseI GCWGC 2 cut(s) 930, 962
TspDTI ATGAA 7 cut(s) 152, 442, 893, 917, 953, 1013, 1077
TspRI CASTG 4 cut(s) 237, 577, 1039, 1166
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 3 cut(s) 442, 679, 757
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 988
XmnI GAANNNNTTC 1 cut(s) 154
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.