RchiOBHm_Chr2g0091391

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011073
Physical Location & Seq
Reverse (-)
5058137 .. 5059447
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46657

Sequence Viewer

Length: 1311 bp
ATGATTAGAATCTTGGGTCAGTTTCAAGATTATTTCTTTCCAATGTTTCGTTCGAGCTTGATTGTTTTGCTTCTCTTTATTTCTGTGCCATTCATGGATGCTGAAGCACAATCTGTAGGCGGTTATGATTCTGAAACAGATCAATCTAATTTTCAACCAAGTGTTGCTGTGGTTATAGGTATTTTAGCCCTCATGTTTGCTCTGACTTTTGTTCTGTTAGTGTATGCCAAGTTCTGCCATAGAAGAGTTTCTGTTCATGGCAACCATCACTTGGAAGGGGGGCGTATCGGCACAAGCTCTCGGTTTTCTGGTATTGACAAGACAGTGATTGAGGCACTTCCCTTTTTCAGATTCTCTAATCTTAAAGGGTCAAAGGAAGGGCTAGAATGTGCAGTCTGCCTATCAAAATTTGAAGACATCGAAGTCCTCAGATTGCTTCCCAAGTGCAAGCATGCTTTCCACATTGATTGCATCGATCACTGGCTTGAGAAGCACTCCAGCTGTCCTCTTTGCAGGCACAAGGTCAGTTCTGAGGACCTCACGTTCATTGCATACTCCAACAGTATGAGAGTCTTGTGGGATAGCCAATCAGAGCGGCGAGAAGACTCAAACATTGAGCTCTTTGTTCAGAGAGAGGAAGACCACCGCGGCTCTTCAAGGTTCAGTGTGGGAAGTAGCTTTCGGAAAATGGAGAAGGGCGTTGATAAAGGAGAGGAACTGTTGATCCAAGAAGAAGCTGGGATTCTCAAGGAGGATGATGATGATGACAAGGTCCTGCACAGACACAAGCACAAAATTGTTGTGTCTGATTTTGTGTTCCAGAACAGATGGAGTAATCTGAGTTCCTCGGACCTCATTTTCTTGAACTCGGAGATGCTTAATACAATGTCAAGCAGTAGATTCTCCACATCAGCTCGTTTAAACAACGAGCAGTTTTCGCCGAAGGAAGCGACAGGAAATGATGAGATTATGAAGATTAGGGAGGAGATGGAGATGAAGAGATTGTTCGAAAACAAAGTCAGCAGCACAATGAACAAAAACAGTACTACTTTAGCAGCTTCAACCCTACCAACTCATGCCTCGAGTTCTATGAATCAAGGTGAGAAGAGATCAATGTCAGAAATCACTGCTCTTTCGAGATTTGGAAACTGGGGTATCAAGAACAGAACCAAAGAGCCTTCATTGCTTGGTAACAATGTGAAAGAGGAAAGAATGAGGCGGCTTTGGTTGCCGATTGCCAGAAGAACAGTCCAATGGTTTGCCAATAGAGAGAAAAGGTCTGAACAGCCTCAACATTCTTCAAATGTGTAA

Protein Analysis

436

Amino Acids

50.0

Weight (kDa)

7.69

Isoelectric Point (pI)

64.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 129 - 172 7.3e-13 Ring finger domain
zf-RING_11 PF17123 129 - 158 4.4e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 129 - 171 2e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 130 - 171 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 132 - 172 2.7e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 145 - 175 7.8e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20030 AT4G28890
fragaria_vesca FvH4_1g05750
malus_domestica MD02G1058600.v1.1 MD02G1058700.v1.1 MD15G1192900.v1.1 MD15G1193000.v1.1
prunus_persica Prupe.7G224300_v2.0.a1
pyrus_communis pycom02g04750 pycom15g17270 pycom15g17280
rosa_chinensis RchiOBHm_Chr2g0091391
rosa_laevigata RLG00000016220
rosa_multiflora Rmu_ssc0000333.1_g000003
rosa_rugosa Rorug02G0017400
rosa_samantha Rh2AG062400 Rh2BG061400 Rh2CG063500 Rh2DG062000
rosa_wichuraiana Rw2G005780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 271
AccBSI CCGCTC 1 cut(s) 595
AccII CGCG 1 cut(s) 648
AciI CCGC 5 cut(s) 120, 595, 646, 648, 1219
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 1 cut(s) 1045
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 7 cut(s) 26, 155, 413, 657, 865, 1062, 1302
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AloI GAACNNNNNNTCC 4 cut(s) 527, 559, 708, 740
AluBI AGCT 8 cut(s) 57, 297, 501, 619, 678, 737, 914, 1058
AluI AGCT 8 cut(s) 57, 297, 501, 619, 678, 737, 914, 1058
Alw21I GWGCWC 1 cut(s) 621
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 1081
ApeKI GCWGC 2 cut(s) 1023, 1055
ApoI RAATTY 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 1002, 1034
Asp700I GAANNNNTTC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 535, 772, 850
AsuHPI GGTGA 1 cut(s) 1112
AsuII TTCGAA 1 cut(s) 1008
AvaI CYCGRG 1 cut(s) 1081
AvaII GGWCC 3 cut(s) 535, 772, 850
BaeI ACNNNNGTAYC 2 cut(s) 1138, 1171
BanII GRGCYC 1 cut(s) 621
BbsI GAAGAC 3 cut(s) 420, 609, 645
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 2 cut(s) 1035, 1067
BccI CCATC 3 cut(s) 273, 822, 982
BfaI CTAG 1 cut(s) 383
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 5 cut(s) 596, 649, 1024, 1056, 1220
BlsI GCNGC 5 cut(s) 597, 650, 1025, 1057, 1221
BmcAI AGTACT 1 cut(s) 1045
Bme18I GGWCC 3 cut(s) 535, 772, 850
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 535, 772, 850
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 3 cut(s) 88, 480, 864
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 3 cut(s) 420, 609, 645
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 481
Bpu14I TTCGAA 1 cut(s) 1008
BpuEI CTTGAG 2 cut(s) 506, 731
Bsa29I ATCGAT 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 646, 846
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 2 cut(s) 485, 1154
Bse3DI GCAATG 2 cut(s) 546, 1181
Bse8I GATNNNNATC 1 cut(s) 8
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 646, 846
BseGI GGATG 2 cut(s) 103, 760
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 2 cut(s) 546, 1181
BseMII CTCAG 3 cut(s) 442, 522, 830
BseNI ACTGG 2 cut(s) 485, 1154
BseRI GAGGAG 1 cut(s) 998
BseXI GCAGC 2 cut(s) 1035, 1067
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 2 cut(s) 411, 761
Bsh1236I CGCG 1 cut(s) 648
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 1081
BslI CCNNNNNNNGG 1 cut(s) 271
BsoBI CYCGRG 1 cut(s) 1081
Bsp119I TTCGAA 1 cut(s) 1008
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 4 cut(s) 139, 475, 723, 1109
BspACI CCGC 5 cut(s) 120, 595, 646, 648, 1219
BspCNI CTCAG 3 cut(s) 441, 523, 831
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 1 cut(s) 648
BspPI GGATC 1 cut(s) 718
BspQI GCTCTTC 1 cut(s) 658
BspT104I TTCGAA 1 cut(s) 1008
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 546, 1181
BsrI ACTGG 2 cut(s) 485, 1154
BssECI CCNNGG 2 cut(s) 646, 846
BssMI GATC 4 cut(s) 139, 475, 723, 1109
Bst4CI ACNGT 5 cut(s) 325, 563, 720, 1043, 1249
Bst6I CTCTTC 4 cut(s) 238, 658, 992, 1100
BstBI TTCGAA 1 cut(s) 1008
BstC8I GCNNGC 3 cut(s) 449, 453, 515
BstDEI CTNAG 3 cut(s) 428, 531, 839
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 2 cut(s) 103, 760
BstFNI CGCG 1 cut(s) 648
BstKTI GATC 4 cut(s) 142, 478, 726, 1112
BstMBI GATC 4 cut(s) 139, 475, 723, 1109
BstMWI GCNNNNNNNGC 4 cut(s) 490, 937, 1183, 1228
BstNSI RCATGY 1 cut(s) 455
BstSFI CTRYAG 1 cut(s) 114
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 2 cut(s) 1035, 1067
BstV2I GAAGAC 3 cut(s) 420, 609, 645
Bsu15I ATCGAT 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 2 cut(s) 103, 760
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 4 cut(s) 330, 478, 670, 1125
Cac8I GCNNGC 3 cut(s) 449, 453, 515
Cfr13I GGNCC 3 cut(s) 535, 772, 850
Cfr42I CCGCGG 1 cut(s) 649
ClaI ATCGAT 1 cut(s) 474
Csp6I GTAC 1 cut(s) 1044
CspCI CAANNNNNGTGG 2 cut(s) 449, 484
CviAII CATG 5 cut(s) 94, 193, 257, 452, 1076
CviQI GTAC 1 cut(s) 1044
DdeI CTNAG 3 cut(s) 428, 531, 839
DpnI GATC 4 cut(s) 141, 477, 725, 1111
DpnII GATC 4 cut(s) 139, 475, 723, 1109
DraI TTTAAA 1 cut(s) 921
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
Eam1104I CTCTTC 4 cut(s) 238, 658, 992, 1100
EarI CTCTTC 4 cut(s) 238, 658, 992, 1100
Ecl136II GAGCTC 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 621
Eco47I GGWCC 3 cut(s) 535, 772, 850
Eco53kI GAGCTC 1 cut(s) 619
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 1081
EcoICRI GAGCTC 1 cut(s) 619
EcoO109I RGGNCCY 2 cut(s) 535, 772
EcoT38I GRGCYC 1 cut(s) 621
FaeI CATG 5 cut(s) 97, 196, 260, 455, 1079
FatI CATG 5 cut(s) 93, 192, 256, 451, 1075
Fnu4HI GCNGC 5 cut(s) 596, 649, 1024, 1056, 1220
FokI GGATG 2 cut(s) 110, 767
FriOI GRGCYC 1 cut(s) 621
Fsp4HI GCNGC 5 cut(s) 596, 649, 1024, 1056, 1220
FspBI CTAG 1 cut(s) 383
GluI GCNGC 5 cut(s) 596, 649, 1024, 1056, 1220
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 1 cut(s) 481
Hin1II CATG 5 cut(s) 97, 196, 260, 455, 1079
HinfI GANTC 8 cut(s) 9, 128, 351, 570, 605, 742, 900, 1093
HphI GGTGA 1 cut(s) 1112
Hpy188III TCNNGA 5 cut(s) 26, 820, 862, 1137, 1159
HpyAV CCTTC 5 cut(s) 269, 371, 688, 937, 1188
HpyCH4III ACNGT 5 cut(s) 325, 563, 720, 1043, 1249
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 6 cut(s) 392, 447, 471, 513, 551, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 490, 937, 1183, 1228
HpyF3I CTNAG 3 cut(s) 428, 531, 839
HpySE526I ACGT 1 cut(s) 542
Hsp92II CATG 5 cut(s) 97, 196, 260, 455, 1079
KspI CCGCGG 1 cut(s) 649
Kzo9I GATC 4 cut(s) 139, 475, 723, 1109
LguI GCTCTTC 1 cut(s) 658
Lsp1109I GCAGC 2 cut(s) 1035, 1067
LweI GCATC 3 cut(s) 88, 480, 864
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 1 cut(s) 1190
MalI GATC 4 cut(s) 141, 477, 725, 1111
MbiI CCGCTC 1 cut(s) 595
MboI GATC 4 cut(s) 139, 475, 723, 1109
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 3 cut(s) 148, 407, 795
MlyI GAGTC 2 cut(s) 579, 599
MmeI TCCRAC 1 cut(s) 582
MroXI GAANNNNTTC 1 cut(s) 247
MseI TTAA 3 cut(s) 363, 879, 920
MspA1I CMGCKG 2 cut(s) 501, 648
MssI GTTTAAAC 1 cut(s) 921
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 4 cut(s) 490, 937, 1183, 1228
NdeII GATC 4 cut(s) 139, 475, 723, 1109
NlaIII CATG 5 cut(s) 97, 196, 260, 455, 1079
NspI RCATGY 1 cut(s) 455
NspV TTCGAA 1 cut(s) 1008
PaeI GCATGC 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 1081
PciSI GCTCTTC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 247
PfeI GAWTC 6 cut(s) 9, 128, 351, 742, 900, 1093
PflFI GACNNNGTC 1 cut(s) 770
PflMI CCANNNNNTGG 1 cut(s) 271
PkrI GCNGC 5 cut(s) 597, 650, 1025, 1057, 1221
PleI GAGTC 2 cut(s) 578, 599
PmeI GTTTAAAC 1 cut(s) 921
PpsI GAGTC 2 cut(s) 578, 599
PpuMI RGGWCCY 2 cut(s) 535, 772
Psp124BI GAGCTC 1 cut(s) 621
Psp5II RGGWCCY 2 cut(s) 535, 772
PspFI CCCAGC 1 cut(s) 737
PspPI GGNCC 3 cut(s) 535, 772, 850
PspPPI RGGWCCY 2 cut(s) 535, 772
PspXI VCTCGAGB 1 cut(s) 1081
PsrI GAACNNNNNNTAC 4 cut(s) 215, 247, 826, 858
PsyI GACNNNGTC 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 501
RsaI GTAC 1 cut(s) 1045
RsaNI GTAC 1 cut(s) 1044
SacI GAGCTC 1 cut(s) 621
SacII CCGCGG 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 658
SaqAI TTAA 3 cut(s) 363, 879, 920
SatI GCNGC 5 cut(s) 596, 649, 1024, 1056, 1220
Sau3AI GATC 4 cut(s) 139, 475, 723, 1109
Sau96I GGNCC 3 cut(s) 535, 772, 850
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 2 cut(s) 579, 599
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 3 cut(s) 88, 480, 864
SfcI CTRYAG 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 1081
Sfr303I CCGCGG 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 1008
SgrBI CCGCGG 1 cut(s) 649
SinI GGWCC 3 cut(s) 535, 772, 850
SlaI CTCGAG 1 cut(s) 1081
SmlI CTYRAG 3 cut(s) 485, 746, 1081
SmoI CTYRAG 3 cut(s) 485, 746, 1081
SphI GCATGC 1 cut(s) 455
Sse9I AATT 3 cut(s) 148, 407, 795
SsiI CCGC 5 cut(s) 120, 595, 646, 648, 1219
SspMI CTAG 1 cut(s) 383
SstI GAGCTC 1 cut(s) 621
TaaI ACNGT 5 cut(s) 325, 563, 720, 1043, 1249
TaiI ACGT 1 cut(s) 545
TaqI TCGA 6 cut(s) 53, 420, 474, 1008, 1082, 1136
TasI AATT 3 cut(s) 148, 407, 795
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 3 cut(s) 598, 651, 1222
TfiI GAWTC 6 cut(s) 9, 128, 351, 742, 900, 1093
Tru1I TTAA 3 cut(s) 363, 879, 920
Tru9I TTAA 3 cut(s) 363, 879, 920
TscAI CASTG 4 cut(s) 330, 485, 670, 1132
TseI GCWGC 2 cut(s) 1023, 1055
TspDTI ATGAA 8 cut(s) 82, 245, 535, 986, 1010, 1046, 1106, 1170
TspRI CASTG 4 cut(s) 330, 485, 670, 1132
Tth111I GACNNNGTC 1 cut(s) 770
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 3 cut(s) 535, 772, 850
XapI RAATTY 1 cut(s) 407
XceI RCATGY 1 cut(s) 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 734
XhoI CTCGAG 1 cut(s) 1081
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 1 cut(s) 383
ZrmI AGTACT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.