FvH4_1g06030

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005821
Physical Location & Seq
Reverse (-)
3219675 .. 3220541
867 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06030.t1

Sequence Viewer

Length: 366 bp
ATGGCTAGTTTATCCAGAAACACATTTCTATCAGACGCGTACGGGATCGATCTGTGCTTTGATCTAGACGACGCGCTGACGCTGCCGGGGAATGAAGACGGATACCGGACTAGTTCAATAACGTCGCCGGAAAGTGATAGTGCTGGTGATGCTTCGGTGGCGGACTTGCCGACGGTTGCTGCAGCGGAAGGTGTCATTTGCTCGGTCTGTGTCGAAGGGTTTCGAGCAGAAGAAGAAGGAGGAGGTGGTCGAGGTAAGCAAGTCCCTTGTGGCCATGTCTATCACGAAACTTGCATAGCAAAGTGGCTTTCACACTCGAACTCTTGCCCTCTCTGCCGCTCCACCGTCTTCCATCCTCATCAGTAA

Protein Analysis

122

Amino Acids

12.77

Weight (kDa)

4.62

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 67 - 113 1.4e-13 Ring finger domain
zf-C3HC4 PF00097 67 - 112 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 67 - 110 2.4e-06 RING-type zinc-finger
zf-RING_5 PF14634 67 - 113 5.6e-06 zinc-RING finger domain
zf-rbx1 PF12678 87 - 113 7.4e-09 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 88 - 112 6.4e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 339
AccII CGCG 2 cut(s) 38, 74
AciI CCGC 3 cut(s) 161, 185, 337
AclWI GGATC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 1 cut(s) 117
AhlI ACTAGT 1 cut(s) 110
AlwI GGATC 1 cut(s) 53
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 3 cut(s) 82, 179, 182
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 158
BalI TGGCCA 1 cut(s) 273
BbsI GAAGAC 2 cut(s) 102, 340
BbvI GCAGC 3 cut(s) 69, 166, 194
BccI CCATC 1 cut(s) 360
BciVI GTATCC 1 cut(s) 95
BcnI CCSGG 1 cut(s) 87
BcuI ACTAGT 1 cut(s) 110
BfaI CTAG 3 cut(s) 6, 65, 111
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 95
BisI GCNGC 4 cut(s) 83, 180, 183, 337
BlsI GCNGC 4 cut(s) 84, 181, 184, 338
Bme1390I CCNGG 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 102, 340
BpuMI CCSGG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 86
BsaWI WCCGGW 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 86
BseGI GGATG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 3 cut(s) 69, 166, 194
Bsh1236I CGCG 2 cut(s) 38, 74
BshFI GGCC 1 cut(s) 273
BshVI ATCGAT 1 cut(s) 48
BsiSI CCGG 3 cut(s) 86, 106, 128
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 273
Bsp143I GATC 3 cut(s) 45, 49, 61
BspACI CCGC 3 cut(s) 161, 185, 337
BspANI GGCC 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 48
BspFNI CGCG 2 cut(s) 38, 74
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 3 cut(s) 45, 49, 61
Bst4CI ACNGT 2 cut(s) 175, 346
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 2 cut(s) 38, 74
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 3 cut(s) 48, 52, 64
BstMBI GATC 3 cut(s) 45, 49, 61
BstMWI GCNNNNNNNGC 4 cut(s) 82, 149, 158, 333
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 2 cut(s) 38, 74
BstV1I GCAGC 3 cut(s) 69, 166, 194
BstV2I GAAGAC 2 cut(s) 102, 340
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 1 cut(s) 95
BsuRI GGCC 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 48
BtsCI GGATG 1 cut(s) 352
CfoI GCGC 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 3 cut(s) 44, 80, 88
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 275
CviJI RGCY 3 cut(s) 5, 273, 307
CviKI_1 RGCY 3 cut(s) 5, 273, 307
CviQI GTAC 1 cut(s) 40
DpnI GATC 3 cut(s) 47, 51, 63
DpnII GATC 3 cut(s) 45, 49, 61
EaeI YGGCCR 1 cut(s) 271
EciI GGCGGA 1 cut(s) 176
FaeI CATG 1 cut(s) 278
FaiI YATR 2 cut(s) 276, 296
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 4 cut(s) 83, 180, 183, 337
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 83, 180, 183, 337
FspBI CTAG 3 cut(s) 6, 65, 111
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 83, 180, 183, 337
HaeIII GGCC 1 cut(s) 273
HapII CCGG 3 cut(s) 86, 106, 128
HgaI GACGC 3 cut(s) 44, 80, 88
HhaI GCGC 1 cut(s) 76
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HpaII CCGG 3 cut(s) 86, 106, 128
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 3 cut(s) 15, 65, 284
Hpy99I CGWCG 3 cut(s) 74, 127, 175
HpyAV CCTTC 3 cut(s) 182, 209, 230
HpyCH4III ACNGT 2 cut(s) 175, 346
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 2 cut(s) 182, 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 82, 149, 158, 333
HpySE526I ACGT 1 cut(s) 122
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 3 cut(s) 45, 49, 61
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 5 cut(s) 28, 99, 119, 129, 141
Lsp1109I GCAGC 3 cut(s) 69, 166, 194
LweI GCATC 1 cut(s) 139
MaeI CTAG 3 cut(s) 6, 65, 111
MaeII ACGT 1 cut(s) 122
MalI GATC 3 cut(s) 47, 51, 63
MbiI CCGCTC 1 cut(s) 339
MboI GATC 3 cut(s) 45, 49, 61
MboII GAAGA 4 cut(s) 107, 242, 245, 340
MlsI TGGCCA 1 cut(s) 273
MluI ACGCGT 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 273
MnlI CCTC 5 cut(s) 233, 236, 245, 339, 366
Mox20I TGGCCA 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 219
MscI TGGCCA 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 3 cut(s) 86, 106, 128
MspR9I CCNGG 1 cut(s) 87
MvnI CGCG 2 cut(s) 38, 74
MwoI GCNNNNNNNGC 4 cut(s) 82, 149, 158, 333
NciI CCSGG 1 cut(s) 87
NdeII GATC 3 cut(s) 45, 49, 61
NlaIII CATG 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 219
Pfl23II CGTACG 1 cut(s) 39
PkrI GCNGC 4 cut(s) 84, 181, 184, 338
PspLI CGTACG 1 cut(s) 39
PstI CTGCAG 1 cut(s) 184
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SatI GCNGC 4 cut(s) 83, 180, 183, 337
Sau3AI GATC 3 cut(s) 45, 49, 61
ScrFI CCNGG 1 cut(s) 87
SetI ASST 4 cut(s) 125, 193, 247, 256
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 180
SpeI ACTAGT 1 cut(s) 110
SsiI CCGC 3 cut(s) 161, 185, 337
SspMI CTAG 3 cut(s) 6, 65, 111
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 175, 346
TaiI ACGT 1 cut(s) 125
TaqI TCGA 5 cut(s) 48, 213, 223, 250, 317
TaqII GACCGA 1 cut(s) 193
TauI GCSGC 1 cut(s) 339
TseI GCWGC 3 cut(s) 82, 179, 182
TspDTI ATGAA 1 cut(s) 108
TspGWI ACGGA 1 cut(s) 114
XbaI TCTAGA 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 3 cut(s) 6, 65, 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.