MD15G1195700.v1.1

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005821
Physical Location & Seq
Reverse (-)
15544011 .. 15544508
498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1195700.v1.1.491

Sequence Viewer

Length: 498 bp
ATGTTTATCCAACCATCACCATACAAAACAATTAAAACTATATATTGGTTTTCAAGTTTTCCATTCAAGTTCTCTCTCTCTTCTTTCTCTCTTCTCTCTCTCATGGGTACCATCTCCAACCCTATTAGAAGAACAGCAAGCCATGCAATCGATATGTACTTTGATTTGGACGAAGCCCTGACGCTGCCACCTGAAGATCATATATGCTACCGTGGGAATAACTATAATATTGCAACGTCGACGGCATACACTTTGGCAGCCAACATGCCGACGGCGGTGGGTGCAGAAGAAGTTAATGAAGGTGTAATTTGTTCAATTTGTGCTGTATGTTTTCATCACGGATATTCTGATCATGATAATGAGTTTGCTGCTAAGCAAGTCCCTTGTCGCCATGTCTACCACCAAACTTGCATAGCAAACTGGCTCTCCAATTCTAACTCTTGCCCTCTCTGCCGCTCCACAATCATTCCGATTGATCGACGCCCAATAACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

166

Amino Acids

18.55

Weight (kDa)

6.35

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 104 - 152 5.6e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 107 - 152 4.3e-10 Ring finger domain
zf-C3HC4 PF00097 107 - 151 2.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 124 - 151 2.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 456
AccI GTMKAC 2 cut(s) 239, 396
AciI CCGC 2 cut(s) 275, 454
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 481
AfaI GTAC 2 cut(s) 109, 158
AgsI TTSAA 3 cut(s) 54, 67, 315
ApeKI GCWGC 3 cut(s) 184, 257, 368
Asp718I GGTACC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 9
BanI GGYRCC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 171, 269, 355
BccI CCATC 2 cut(s) 22, 119
BceAI ACGGC 2 cut(s) 258, 288
BclI TGATCA 1 cut(s) 349
BisI GCNGC 4 cut(s) 185, 258, 369, 454
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 4 cut(s) 186, 259, 370, 455
BmiI GGNNCC 1 cut(s) 109
Bpu1102I GCTNAGC 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bse1I ACTGG 1 cut(s) 425
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 211
BseNI ACTGG 1 cut(s) 425
BseXI GCAGC 3 cut(s) 171, 269, 355
BsgI GTGCAG 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 107
BshVI ATCGAT 1 cut(s) 150
BslFI GGGAC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 365
Bsp143I GATC 3 cut(s) 196, 349, 475
Bsp1720I GCTNAGC 1 cut(s) 372
BspACI CCGC 2 cut(s) 275, 454
BspDI ATCGAT 1 cut(s) 150
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 109
BspT107I GGYRCC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 456
BsrI ACTGG 1 cut(s) 425
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 3 cut(s) 196, 349, 475
BssNI GRCGYC 1 cut(s) 481
Bst4CI ACNGT 1 cut(s) 212
Bst6I CTCTTC 2 cut(s) 85, 96
BstACI GRCGYC 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 372
BstDSI CCRYGG 1 cut(s) 211
BstKTI GATC 3 cut(s) 199, 352, 478
BstMBI GATC 3 cut(s) 196, 349, 475
BstMWI GCNNNNNNNGC 3 cut(s) 143, 281, 450
BstNSI RCATGY 1 cut(s) 268
BstV1I GCAGC 3 cut(s) 171, 269, 355
Bsu15I ATCGAT 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 211
Cac8I GCNNGC 1 cut(s) 139
CciI TCATGA 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 2 cut(s) 190, 489
Csp6I GTAC 2 cut(s) 108, 157
CspCI CAANNNNNGTGG 2 cut(s) 389, 424
CviAII CATG 5 cut(s) 103, 143, 265, 353, 392
CviJI RGCY 4 cut(s) 141, 176, 260, 424
CviKI_1 RGCY 4 cut(s) 141, 176, 260, 424
CviQI GTAC 2 cut(s) 108, 157
DdeI CTNAG 1 cut(s) 372
DpnI GATC 3 cut(s) 198, 351, 477
DpnII GATC 3 cut(s) 196, 349, 475
Eam1104I CTCTTC 2 cut(s) 85, 96
EarI CTCTTC 2 cut(s) 85, 96
Eco57I CTGAAG 1 cut(s) 213
FaeI CATG 5 cut(s) 106, 146, 268, 356, 395
FaqI GGGAC 1 cut(s) 365
FatI CATG 5 cut(s) 102, 142, 264, 352, 391
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 2 cut(s) 239, 396
Fnu4HI GCNGC 4 cut(s) 185, 258, 369, 454
Fsp4HI GCNGC 4 cut(s) 185, 258, 369, 454
GluI GCNGC 4 cut(s) 185, 258, 369, 454
HgaI GACGC 2 cut(s) 190, 489
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 5 cut(s) 106, 146, 268, 356, 395
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 2 cut(s) 240, 397
Hpy188I TCNGA 2 cut(s) 349, 471
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 2 cut(s) 240, 397
Hpy99I CGWCG 4 cut(s) 241, 244, 274, 483
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 4 cut(s) 146, 233, 284, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 281, 450
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 1 cut(s) 236
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 5 cut(s) 106, 146, 268, 356, 395
KpnI GGTACC 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 3 cut(s) 196, 349, 475
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 3 cut(s) 191, 204, 406
Lsp1109I GCAGC 3 cut(s) 171, 269, 355
MaeII ACGT 1 cut(s) 236
MalI GATC 3 cut(s) 198, 351, 477
MbiI CCGCTC 1 cut(s) 456
MboI GATC 3 cut(s) 196, 349, 475
MboII GAAGA 5 cut(s) 72, 83, 141, 206, 299
MluCI AATT 5 cut(s) 30, 306, 315, 430, 493
MmeI TCCRAC 2 cut(s) 34, 141
MnlI CCTC 1 cut(s) 456
MseI TTAA 3 cut(s) 33, 294, 496
MslI CAYNNNNRTG 1 cut(s) 357
MwoI GCNNNNNNNGC 3 cut(s) 143, 281, 450
NdeII GATC 3 cut(s) 196, 349, 475
NlaIII CATG 5 cut(s) 106, 146, 268, 356, 395
NlaIV GGNNCC 1 cut(s) 109
NspI RCATGY 1 cut(s) 268
PagI TCATGA 1 cut(s) 352
PkrI GCNGC 4 cut(s) 186, 259, 370, 455
PspN4I GGNNCC 1 cut(s) 109
RsaI GTAC 2 cut(s) 109, 158
RsaNI GTAC 2 cut(s) 108, 157
RseI CAYNNNNRTG 1 cut(s) 357
SalI GTCGAC 1 cut(s) 238
SaqAI TTAA 3 cut(s) 33, 294, 496
SatI GCNGC 4 cut(s) 185, 258, 369, 454
Sau3AI GATC 3 cut(s) 196, 349, 475
SetI ASST 3 cut(s) 193, 239, 304
SgrDI CGTCGACG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 357
Sse9I AATT 5 cut(s) 30, 306, 315, 430, 493
SsiI CCGC 2 cut(s) 275, 454
SspI AATATT 1 cut(s) 229
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 239
TaqI TCGA 3 cut(s) 150, 239, 478
TasI AATT 5 cut(s) 30, 306, 315, 430, 493
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 456
Tru1I TTAA 3 cut(s) 33, 294, 496
Tru9I TTAA 3 cut(s) 33, 294, 496
TseI GCWGC 3 cut(s) 184, 257, 368
TspDTI ATGAA 2 cut(s) 312, 323
TspGWI ACGGA 1 cut(s) 354
XceI RCATGY 1 cut(s) 268
XmiI GTMKAC 2 cut(s) 239, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.