Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Reverse (-)

3835515 .. 3838870

Gene Structure 3356 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g07260.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGGCCGATCCTTCTTCATGTGCTCATTGCAGCATCTATGAGAGGTTGGACTGGACGATGTCTGATCACCGTGTCGAGATCTTGGCTGTGCTTTCTAAGCTTGAAAGCAATGGCAAAGGGTTTGTGCAACCCCAGCAACTTGTTGCTGTGTTTCAAGCAATCCCTGAAGCCTTTGGTGAGGTTTCAAAGAACATTCAGGAAGCTCTTGTTCTATCTCCATACCTAGCTTTGGCTATTCGTCCTAGGCCTGGTGTTTGGGAACACATCAGAATCAACATGAAGACACTCGATGTTGAGGAGTTGCTTGTGGATGAGTACTTGATGATGAAGGAAGAACTCGTCGATGGAAGTGCTAAAGGAGACTTTGTGCTGGAGTTGGACTTTGAGCCATTCAATGCATCAGTTCCTAAACCAATTCTTTCCAAGTCTATTGGAAACGGCGTTGAGTTCCTCAACCGCCATATTTCTGCAAAGCTCTTCACTGAGCCGGAGAGCATGCAACCGGAGTTGCTTGAGTTTCTCCGTATTCACACCCATGACGGCAAGAACCTGATGCTGAATGACAACATTGAGGATGTGGAGTCCCTCCAACATGTTTTGATGGAGGTGGAAGAGTATCTCCTGACACTCTCACCCGAAACCGCATATGAAGCTTTCGAGCAGAAGTTGCAGGATCTTGGCTTGGAAAGAGGTTGGGGAAGCAATGCTGGCCGTGTGCTTGAAACTATCCAGCTTCTCTTGGAGCTTCTTGAGGCACCTGATGCTTGCAATCTCGAGAAATTCCTTAGCAAGATACCCTTGGTCTTTAATGTTGTGATCATGTCTCCCCATGGCTATTTTGCACAAGACAATGTCTTGGGATATCCTGACACTGGCGGGCAGGTTGTTTATATCTTGGATCAAGTTCGTGCTTTGGAGAATGAGATGCTCAAGCGTATCAAGGAACAAGGCCTTGACATCACCCCTCGCATTCTTATTGTTACAAGGCTCCTCCCTGATGCTGTTGGGACTACTTGTGGTCAGCGTCTTGAGAAGGTTTATGGAACTCAATATTCGGATATTATTCGTGTCCCTTTCAGAACAGAGAAGGGAATTGTCCGCAGATGGATCTCAAGATTCGAAGTCTGGCCCTATCTAGAGGCTTACACTGAGGATGTTGCAACAGAACTTCTCAAGGAGTTCCAGGGCAAGCCTGATCTCATTGTCGGAAACTATAGTGATGGAAACATTGTGGCCTCATTGTTGGCACACAAGTTTGATGTTACTCAGTGCACCATTGCTCATGCCTTGGAGAAGACAAAATACCCTGATTCTGACTTGTACTGGAAGAAACTCGAGGACAAGTACCACTTCTCTTGCCAGTTCACAGCTGATCTCATAGCCATGAACCAGACCGATTTCATTATCACAAGTACCTTCCAAGAGATTGCTGGAAACAAAGAGACTGTTGGTCAATATGAGAGTCACCTTGCCTTCACAATGCCTGGCCTCTACCGCGTTGTCAGTGGTATCGATTGCTTTGATCCCAAGTTCAACATTGTTTCCCCAGGAGCTGACATGGACATTTACTTCCCATACACTGAGAAGGAAAGAAGGTTGACATCATTCCACTCTGAAATCGAAGAGCTTCTCTACAACCAAGTGGAGAACAAGGAACACTTGTGTGTTTTGAATGACAAGAACAAGCCTATCATCTTCACAATGGCAAGGCTTGACCGTGTGAAGAACATCACCGGGCTTGTGGAATGGTATGGCAAGAATGCCAAGCTTAGGGAGTTGGTTAACCTTGTGGTTGTGGCTGGGGATCGGCGCAAGGAGTCCAAGGACAACGAGGAGAAGGCTGAGATGAAGAAGATGTATGAGCTAATTGACACCTACAAGTTGAATGGACAGTTCAGATGGATTTCTTCTCAGATGAACCGTGTGAGGAACGGTGAGCTCTACCGCTACATTGCTGACACTAAAGGAGCTTTTGTGCAGCCTGCTGTCTATGAAGCCTTTGGCTTGACTGTTGTGGAGGCAATGACTTGTGGATTGCCTACTTTCGCCACTTGCAAAGGCGGCCCTGCTGAGATTATTGTGAATGGCAAATCCGGCTACCACATTGATCCATATAACGGTGACAAAGCTGCTGATATCCTTGTCGATTTCTTTGAGAAGTGCAAGGCTGATCCTTCTCACTGGGATAAAATTTCCCAAGGAGGCTTGAAGCGTATCGAAGAGAAGTATACTTGGCAGATTTACTCTGAGAGGCTGCTGACCCTGACCGGAGTTTATGGCTTCTGGAAGCAAGTGTCTGACCTTGACGAGAAGCGTCGCTACATCGAAATGTTGTATGCCCTCCGGTATAAGAAATCGGTTGAGTCTGTTCCTCTTGCTGAAGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

797

Amino Acids

91.03

Weight (kDa)

5.1

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	11 - 111	6e-27	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 233	7.7e-30	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	257 - 545	2.2e-165	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	558 - 719	7.2e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	563 - 716	2.3e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	871
AccB1I	GGYRCC	1 cut(s)	754
AccI	GTMKAC	1 cut(s)	2228
AccII	CGCG	1 cut(s)	1497
AciI	CCGC	7 cut(s)	457, 642, 876, 1099, 1495, 1945, 2061
AclWI	GGATC	8 cut(s)	2, 681, 906, 1115, 1517, 1812, 2102, 2165
AcoI	YGGCCR	2 cut(s)	3, 709
AcsI	RAATTY	2 cut(s)	779, 2190
AcuI	CTGAAG	1 cut(s)	186
AfaI	GTAC	4 cut(s)	317, 1322, 1346, 1414
AfiI	CCNNNNNNNGG	5 cut(s)	229, 248, 872, 1770, 2117
AflIII	ACRYGT	1 cut(s)	592
AgsI	TTSAA	9 cut(s)	104, 155, 186, 394, 722, 1534, 1672, 1885, 2209
AhdI	GACNNNNNGTC	1 cut(s)	1449
AjnI	CCWGG	4 cut(s)	247, 1182, 1483, 1546
AleI	CACNNNNGTG	1 cut(s)	1662
Alw21I	GWGCWC	3 cut(s)	25, 1274, 1941
Alw26I	GTCTC	3 cut(s)	354, 828, 1436
Alw44I	GTGCAC	1 cut(s)	1270
AlwI	GGATC	8 cut(s)	2, 681, 906, 1115, 1517, 1812, 2102, 2165
AlwNI	CAGNNNCTG	1 cut(s)	1553
Ama87I	CYCGRG	2 cut(s)	773, 1334
AoxI	GGCC	8 cut(s)	3, 245, 709, 949, 1127, 1233, 1486, 2062
ApaLI	GTGCAC	1 cut(s)	1270
ApeKI	GCWGC	4 cut(s)	30, 1978, 2129, 2254
ApoI	RAATTY	2 cut(s)	779, 2190
Asp700I	GAANNNNTTC	1 cut(s)	1626
AspA2I	CCTAGG	1 cut(s)	242
AspLEI	GCGC	1 cut(s)	1812
AspS9I	GGNCC	2 cut(s)	1128, 2063
AsuC2I	CCSGG	1 cut(s)	1735
AsuHPI	GGTGA	8 cut(s)	59, 188, 624, 952, 1457, 1723, 1946, 2132
AsuII	TTCGAA	1 cut(s)	1119
AvaI	CYCGRG	2 cut(s)	773, 1334
AvrII	CCTAGG	1 cut(s)	242
BaeGI	GKGCMC	1 cut(s)	1274
BanI	GGYRCC	1 cut(s)	754
BanII	GRGCYC	1 cut(s)	1941
BarI	GAAGNNNNNNTAC	4 cut(s)	1286, 1318, 2303, 2335
BbsI	GAAGAC	2 cut(s)	287, 1301
Bbv12I	GWGCWC	3 cut(s)	25, 1274, 1941
BbvI	GCAGC	4 cut(s)	42, 1990, 2116, 2241
BccI	CCATC	5 cut(s)	338, 595, 1098, 1214, 1893
BceAI	ACGGC	3 cut(s)	454, 556, 696
BcgI	CGANNNNNNTGC	2 cut(s)	332, 366
BciT130I	CCWGG	4 cut(s)	249, 1184, 1485, 1548
BclI	TGATCA	2 cut(s)	64, 816
BcnI	CCSGG	1 cut(s)	1735
BcoDI	GTCTC	3 cut(s)	354, 828, 1436
BfaI	CTAG	3 cut(s)	224, 243, 1136
BfmI	CTRYAG	1 cut(s)	1213
BfuAI	ACCTGC	1 cut(s)	871
BglII	AGATCT	1 cut(s)	78
BisI	GCNGC	5 cut(s)	31, 1979, 2062, 2130, 2255
BlnI	CCTAGG	1 cut(s)	242
BlsI	GCNGC	5 cut(s)	32, 1980, 2063, 2131, 2256
BmcAI	AGTACT	1 cut(s)	317
Bme1390I	CCNGG	5 cut(s)	249, 1184, 1485, 1548, 1735
BmeRI	GACNNNNNGTC	1 cut(s)	1449
BmeT110I	CYCGRG	2 cut(s)	773, 1334
BmgT120I	GGNCC	2 cut(s)	1128, 2063
BmiI	GGNNCC	2 cut(s)	756, 989
BmrFI	CCNGG	5 cut(s)	249, 1184, 1485, 1548, 1735
BmrI	ACTGGG	1 cut(s)	2191
BmsI	GCATC	6 cut(s)	42, 407, 543, 751, 915, 988
BmuI	ACTGGG	1 cut(s)	2191
BpiI	GAAGAC	2 cut(s)	287, 1301
BpmI	CTGGAG	1 cut(s)	392
Bpu10I	CCTNAGC	2 cut(s)	785, 1769
Bpu14I	TTCGAA	1 cut(s)	1119
BpuEI	CTTGAG	6 cut(s)	533, 770, 914, 1049, 1096, 1157
BpuMI	CCSGG	1 cut(s)	1735
Bsa29I	ATCGAT	1 cut(s)	1512
BsaJI	CCNNGG	8 cut(s)	242, 798, 829, 1183, 1287, 1546, 1821, 2197
BsaWI	WCCGGW	3 cut(s)	502, 2267, 2343
BsaXI	ACNNNNNCTCC	4 cut(s)	351, 381, 1959, 1989
Bsc4I	CCNNNNNNNGG	5 cut(s)	229, 248, 872, 1770, 2117
Bse1I	ACTGG	5 cut(s)	56, 877, 1328, 1360, 2186
Bse3DI	GCAATG	6 cut(s)	25, 115, 709, 1275, 1950, 2028
BseBI	CCWGG	4 cut(s)	249, 1184, 1485, 1548
BseCI	ATCGAT	1 cut(s)	1512
BseDI	CCNNGG	8 cut(s)	242, 798, 829, 1183, 1287, 1546, 1821, 2197
BseGI	GGATG	3 cut(s)	316, 580, 1159
BseLI	CCNNNNNNNGG	5 cut(s)	229, 248, 872, 1770, 2117
BseMI	GCAATG	6 cut(s)	25, 115, 709, 1275, 1950, 2028
BseMII	CTCAG	8 cut(s)	474, 1140, 1280, 1572, 1833, 1925, 2061, 2238
BseNI	ACTGG	5 cut(s)	56, 877, 1328, 1360, 2186
BseRI	GAGGAG	3 cut(s)	311, 980, 1847
BseSI	GKGCMC	1 cut(s)	1274
BseXI	GCAGC	4 cut(s)	42, 1990, 2116, 2241
BseYI	CCCAGC	2 cut(s)	132, 1799
BsgI	GTGCAG	1 cut(s)	1997
Bsh1236I	CGCG	1 cut(s)	1497
BshFI	GGCC	8 cut(s)	5, 247, 711, 951, 1129, 1235, 1488, 2064
BshNI	GGYRCC	1 cut(s)	754
BshVI	ATCGAT	1 cut(s)	1512
BsiHKAI	GWGCWC	3 cut(s)	25, 1274, 1941
BsiHKCI	CYCGRG	2 cut(s)	773, 1334
BsiSI	CCGG	6 cut(s)	488, 503, 1734, 2094, 2268, 2344
BslFI	GGGAC	3 cut(s)	568, 1021, 1055
BslI	CCNNNNNNNGG	5 cut(s)	229, 248, 872, 1770, 2117
BsmAI	GTCTC	3 cut(s)	354, 828, 1436
BsmFI	GGGAC	3 cut(s)	568, 1021, 1055
BsmI	GAATGC	2 cut(s)	969, 1765
BsnI	GGCC	8 cut(s)	5, 247, 711, 951, 1129, 1235, 1488, 2064
BsoBI	CYCGRG	2 cut(s)	773, 1334
Bsp119I	TTCGAA	1 cut(s)	1119
Bsp1286I	GDGCHC	3 cut(s)	25, 1274, 1941
Bsp19I	CCATGG	1 cut(s)	829
BspACI	CCGC	7 cut(s)	457, 642, 876, 1099, 1495, 1945, 2061
BspANI	GGCC	8 cut(s)	5, 247, 711, 951, 1129, 1235, 1488, 2064
BspCNI	CTCAG	8 cut(s)	475, 1141, 1279, 1573, 1834, 1924, 2062, 2239
BspDI	ATCGAT	1 cut(s)	1512
BspFNI	CGCG	1 cut(s)	1497
BspLI	GGNNCC	2 cut(s)	756, 989
BspMI	ACCTGC	1 cut(s)	871
BspPI	GGATC	8 cut(s)	2, 681, 906, 1115, 1517, 1812, 2102, 2165
BspQI	GCTCTTC	2 cut(s)	482, 1617
BspT104I	TTCGAA	1 cut(s)	1119
BspT107I	GGYRCC	1 cut(s)	754
BsrDI	GCAATG	6 cut(s)	25, 115, 709, 1275, 1950, 2028
BsrI	ACTGG	5 cut(s)	56, 877, 1328, 1360, 2186
BssECI	CCNNGG	8 cut(s)	242, 798, 829, 1183, 1287, 1546, 1821, 2197
BssNAI	GTATAC	1 cut(s)	2229
BssT1I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
Bst1107I	GTATAC	1 cut(s)	2229
Bst2UI	CCWGG	4 cut(s)	249, 1184, 1485, 1548
Bst4CI	ACNGT	8 cut(s)	71, 1447, 1718, 1893, 1922, 1934, 2011, 2120
Bst6I	CTCTTC	5 cut(s)	482, 606, 1617, 2214, 2379
BstAPI	GCANNNNNTGC	2 cut(s)	667, 761
BstBI	TTCGAA	1 cut(s)	1119
BstC8I	GCNNGC	6 cut(s)	497, 709, 766, 878, 1190, 1983
BstDSI	CCRYGG	1 cut(s)	829
BstF5I	GGATG	3 cut(s)	316, 580, 1159
BstFNI	CGCG	1 cut(s)	1497
BstHHI	GCGC	1 cut(s)	1812
BstMAI	GTCTC	3 cut(s)	354, 828, 1436
BstMWI	GCNNNNNNNGC	9 cut(s)	97, 133, 650, 667, 708, 761, 1494, 2061, 2094
BstNI	CCWGG	4 cut(s)	249, 1184, 1485, 1548
BstNSI	RCATGY	2 cut(s)	499, 596
BstSCI	CCNGG	5 cut(s)	247, 1182, 1483, 1546, 1733
BstSFI	CTRYAG	1 cut(s)	1213
BstSLI	GKGCMC	1 cut(s)	1274
BstUI	CGCG	1 cut(s)	1497
BstV1I	GCAGC	4 cut(s)	42, 1990, 2116, 2241
BstV2I	GAAGAC	2 cut(s)	287, 1301
BstX2I	RGATCY	3 cut(s)	78, 673, 1107
BstYI	RGATCY	3 cut(s)	78, 673, 1107
BstZ17I	GTATAC	1 cut(s)	2229
Bsu15I	ATCGAT	1 cut(s)	1512
BsuRI	GGCC	8 cut(s)	5, 247, 711, 951, 1129, 1235, 1488, 2064
BsuTUI	ATCGAT	1 cut(s)	1512
BtgI	CCRYGG	1 cut(s)	829
BtsCI	GGATG	3 cut(s)	316, 580, 1159
BtsIMutI	CAGTG	7 cut(s)	480, 870, 1146, 1274, 1510, 1578, 2179
BveI	ACCTGC	1 cut(s)	871
Cac8I	GCNNGC	6 cut(s)	497, 709, 766, 878, 1190, 1983
CaiI	CAGNNNCTG	1 cut(s)	1553
CfoI	GCGC	1 cut(s)	1812
Cfr13I	GGNCC	2 cut(s)	1128, 2063
ClaI	ATCGAT	1 cut(s)	1512
CseI	GACGC	2 cut(s)	1013, 2303
Csp6I	GTAC	4 cut(s)	316, 1321, 1345, 1413
CspCI	CAANNNNNGTGG	2 cut(s)	1337, 1372
CviQI	GTAC	4 cut(s)	316, 1321, 1345, 1413
DriI	GACNNNNNGTC	1 cut(s)	1449
EaeI	YGGCCR	2 cut(s)	3, 709
Eam1104I	CTCTTC	5 cut(s)	482, 606, 1617, 2214, 2379
Eam1105I	GACNNNNNGTC	1 cut(s)	1449
EarI	CTCTTC	5 cut(s)	482, 606, 1617, 2214, 2379
Ecl136II	GAGCTC	1 cut(s)	1939
Eco130I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
Eco147I	AGGCCT	2 cut(s)	247, 951
Eco24I	GRGCYC	1 cut(s)	1941
Eco32I	GATATC	2 cut(s)	863, 2137
Eco53kI	GAGCTC	1 cut(s)	1939
Eco57I	CTGAAG	1 cut(s)	186
Eco88I	CYCGRG	2 cut(s)	773, 1334
EcoICRI	GAGCTC	1 cut(s)	1939
EcoRII	CCWGG	4 cut(s)	247, 1182, 1483, 1546
EcoRV	GATATC	2 cut(s)	863, 2137
EcoT14I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
EcoT22I	ATGCAT	1 cut(s)	400
EcoT38I	GRGCYC	1 cut(s)	1941
ErhI	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
FalI	AAGNNNNNCTT	6 cut(s)	782, 814, 1334, 1366, 1643, 1675
FaqI	GGGAC	3 cut(s)	568, 1021, 1055
FauI	CCCGC	1 cut(s)	869
FauNDI	CATATG	1 cut(s)	646
FbaI	TGATCA	2 cut(s)	64, 816
FblI	GTMKAC	1 cut(s)	2228
Fnu4HI	GCNGC	5 cut(s)	31, 1979, 2062, 2130, 2255
FokI	GGATG	3 cut(s)	323, 587, 1166
FriOI	GRGCYC	1 cut(s)	1941
Fsp4HI	GCNGC	5 cut(s)	31, 1979, 2062, 2130, 2255
FspBI	CTAG	3 cut(s)	224, 243, 1136
GlaI	GCGC	1 cut(s)	1811
GluI	GCNGC	5 cut(s)	31, 1979, 2062, 2130, 2255
GsaI	CCCAGC	2 cut(s)	136, 1803
GsuI	CTGGAG	1 cut(s)	392
HaeIII	GGCC	8 cut(s)	5, 247, 711, 951, 1129, 1235, 1488, 2064
HapII	CCGG	6 cut(s)	488, 503, 1734, 2094, 2268, 2344
HgaI	GACGC	2 cut(s)	1013, 2303
HhaI	GCGC	1 cut(s)	1812
Hin6I	GCGC	1 cut(s)	1810
HinP1I	GCGC	1 cut(s)	1810
HincII	GTYRAC	2 cut(s)	1599, 1783
HindII	GTYRAC	2 cut(s)	1599, 1783
HindIII	AAGCTT	3 cut(s)	98, 651, 1766
HinfI	GANTC	7 cut(s)	270, 581, 1116, 1310, 1462, 1817, 2363
HpaI	GTTAAC	1 cut(s)	1783
HpaII	CCGG	6 cut(s)	488, 503, 1734, 2094, 2268, 2344
HphI	GGTGA	8 cut(s)	59, 188, 624, 952, 1457, 1723, 1946, 2132
Hpy166II	GTNNAC	5 cut(s)	1272, 1365, 1599, 1783, 2229
Hpy8I	GTNNAC	5 cut(s)	1272, 1365, 1599, 1783, 2229
Hpy99I	CGWCG	2 cut(s)	344, 2319
HpyCH4III	ACNGT	8 cut(s)	71, 1447, 1718, 1893, 1922, 1934, 2011, 2120
HpyF10VI	GCNNNNNNNGC	9 cut(s)	97, 133, 650, 667, 708, 761, 1494, 2061, 2094
HspAI	GCGC	1 cut(s)	1810
Ksp22I	TGATCA	2 cut(s)	64, 816
KspAI	GTTAAC	1 cut(s)	1783
LguI	GCTCTTC	2 cut(s)	482, 1617
LmnI	GCTCC	4 cut(s)	742, 993, 1550, 1967
Lsp1109I	GCAGC	4 cut(s)	42, 1990, 2116, 2241
LweI	GCATC	6 cut(s)	42, 407, 543, 751, 915, 988
MaeI	CTAG	3 cut(s)	224, 243, 1136
MaeIII	GTNAC	4 cut(s)	979, 1261, 1463, 2120
MflI	RGATCY	3 cut(s)	78, 673, 1107
MhlI	GDGCHC	3 cut(s)	25, 1274, 1941
MluCI	AATT	5 cut(s)	414, 779, 1092, 1866, 2190
MlyI	GAGTC	4 cut(s)	590, 1471, 1826, 2372
MmeI	TCCRAC	4 cut(s)	27, 357, 613, 1186
Mph1103I	ATGCAT	1 cut(s)	400
MroXI	GAANNNNTTC	1 cut(s)	1626
MseI	TTAA	2 cut(s)	807, 1782
MslI	CAYNNNNRTG	4 cut(s)	534, 1382, 1662, 2327
MspA1I	CMGCKG	1 cut(s)	1370
MspI	CCGG	6 cut(s)	488, 503, 1734, 2094, 2268, 2344
MspR9I	CCNGG	5 cut(s)	249, 1184, 1485, 1548, 1735
Mva1269I	GAATGC	2 cut(s)	969, 1765
MvaI	CCWGG	4 cut(s)	249, 1184, 1485, 1548
MvnI	CGCG	1 cut(s)	1497
MwoI	GCNNNNNNNGC	9 cut(s)	97, 133, 650, 667, 708, 761, 1494, 2061, 2094
NciI	CCSGG	1 cut(s)	1735
NcoI	CCATGG	1 cut(s)	829
NdeI	CATATG	1 cut(s)	646
NlaIV	GGNNCC	2 cut(s)	756, 989
NmuCI	GTSAC	2 cut(s)	1463, 2120
NsiI	ATGCAT	1 cut(s)	400
NspI	RCATGY	2 cut(s)	499, 596
NspV	TTCGAA	1 cut(s)	1119
OliI	CACNNNNGTG	1 cut(s)	1662
PaeI	GCATGC	1 cut(s)	499
PaeR7I	CTCGAG	2 cut(s)	773, 1334
PceI	AGGCCT	2 cut(s)	247, 951
PciI	ACATGT	1 cut(s)	592
PciSI	GCTCTTC	2 cut(s)	482, 1617
PctI	GAATGC	2 cut(s)	969, 1765
PdmI	GAANNNNTTC	1 cut(s)	1626
PfeI	GAWTC	3 cut(s)	270, 1116, 1310
PflFI	GACNNNGTC	2 cut(s)	58, 851
PkrI	GCNGC	5 cut(s)	32, 1980, 2063, 2131, 2256
PleI	GAGTC	4 cut(s)	589, 1470, 1825, 2371
PpsI	GAGTC	4 cut(s)	589, 1470, 1825, 2371
PscI	ACATGT	1 cut(s)	592
Psp124BI	GAGCTC	1 cut(s)	1941
Psp6I	CCWGG	4 cut(s)	247, 1182, 1483, 1546
PspFI	CCCAGC	2 cut(s)	132, 1799
PspGI	CCWGG	4 cut(s)	247, 1182, 1483, 1546
PspN4I	GGNNCC	2 cut(s)	756, 989
PspPI	GGNCC	2 cut(s)	1128, 2063
PspXI	VCTCGAGB	1 cut(s)	1334
PstNI	CAGNNNCTG	1 cut(s)	1553
PsuI	RGATCY	3 cut(s)	78, 673, 1107
PsyI	GACNNNGTC	2 cut(s)	58, 851
PvuII	CAGCTG	1 cut(s)	1370
RsaI	GTAC	4 cut(s)	317, 1322, 1346, 1414
RsaNI	GTAC	4 cut(s)	316, 1321, 1345, 1413
RseI	CAYNNNNRTG	4 cut(s)	534, 1382, 1662, 2327
SacI	GAGCTC	1 cut(s)	1941
SapI	GCTCTTC	2 cut(s)	482, 1617
SaqAI	TTAA	2 cut(s)	807, 1782
SatI	GCNGC	5 cut(s)	31, 1979, 2062, 2130, 2255
Sau96I	GGNCC	2 cut(s)	1128, 2063
ScaI	AGTACT	1 cut(s)	317
SchI	GAGTC	4 cut(s)	590, 1471, 1826, 2372
ScrFI	CCNGG	5 cut(s)	249, 1184, 1485, 1548, 1735
SduI	GDGCHC	3 cut(s)	25, 1274, 1941
SfaNI	GCATC	6 cut(s)	42, 407, 543, 751, 915, 988
SfcI	CTRYAG	1 cut(s)	1213
Sfr274I	CTCGAG	2 cut(s)	773, 1334
SfuI	TTCGAA	1 cut(s)	1119
SlaI	CTCGAG	2 cut(s)	773, 1334
SmiMI	CAYNNNNRTG	4 cut(s)	534, 1382, 1662, 2327
SmlI	CTYRAG	8 cut(s)	512, 749, 773, 929, 1028, 1111, 1172, 1334
SmoI	CTYRAG	8 cut(s)	512, 749, 773, 929, 1028, 1111, 1172, 1334
SphI	GCATGC	1 cut(s)	499
Sse9I	AATT	5 cut(s)	414, 779, 1092, 1866, 2190
SseBI	AGGCCT	2 cut(s)	247, 951
SsiI	CCGC	7 cut(s)	457, 642, 876, 1099, 1495, 1945, 2061
SspI	AATATT	1 cut(s)	1052
SspMI	CTAG	3 cut(s)	224, 243, 1136
SstI	GAGCTC	1 cut(s)	1941
StuI	AGGCCT	2 cut(s)	247, 951
StyD4I	CCNGG	5 cut(s)	247, 1182, 1483, 1546, 1733
StyI	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
TaaI	ACNGT	8 cut(s)	71, 1447, 1718, 1893, 1922, 1934, 2011, 2120
TaqII	GACCGA	1 cut(s)	1409
TasI	AATT	5 cut(s)	414, 779, 1092, 1866, 2190
TatI	WGTACW	2 cut(s)	315, 1320
TauI	GCSGC	1 cut(s)	2064
TfiI	GAWTC	3 cut(s)	270, 1116, 1310
Tru1I	TTAA	2 cut(s)	807, 1782
Tru9I	TTAA	2 cut(s)	807, 1782
TscAI	CASTG	7 cut(s)	487, 877, 1153, 1274, 1510, 1585, 2186
TseFI	GTSAC	2 cut(s)	1463, 2120
TseI	GCWGC	4 cut(s)	30, 1978, 2129, 2254
Tsp45I	GTSAC	2 cut(s)	1463, 2120
TspDTI	ATGAA	9 cut(s)	6, 293, 341, 663, 1390, 1400, 1862, 1931, 2007
TspGWI	ACGGA	1 cut(s)	512
TspRI	CASTG	7 cut(s)	487, 877, 1153, 1274, 1510, 1585, 2186
Tth111I	GACNNNGTC	2 cut(s)	58, 851
VneI	GTGCAC	1 cut(s)	1270
XapI	RAATTY	2 cut(s)	779, 2190
XbaI	TCTAGA	1 cut(s)	1135
XceI	RCATGY	2 cut(s)	499, 596
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1427
XhoI	CTCGAG	2 cut(s)	773, 1334
XmaJI	CCTAGG	1 cut(s)	242
XmiI	GTMKAC	1 cut(s)	2228
XmnI	GAANNNNTTC	1 cut(s)	1626
XspI	CTAG	3 cut(s)	224, 243, 1136
ZrmI	AGTACT	1 cut(s)	317
Zsp2I	ATGCAT	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.