Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Forward (+)

4121458 .. 4131574

Gene Structure 10117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0053800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGACGAAATATCTCCTCTTCTTCTTCGCTCTCATCCTCCTCGTTTCTCTCTCCTCCCAAACCGCCGACCCGAAAACCCAAAACCCGACCCGAACCCCATCGCCGGCCCACACCGAGCTGACCCGCTACGGCTTCCCAATCGGGCTCCTCCCTTCTCCGGTCAAAAACTACACCGTCAACCCCCTCACCGGCGACTTCGTCGTCGACCTCGGCGACGCGTGCAAGATCACGCTCCCGCCGGACAACTACTTGGCCACGTATTCCAAGAAAGTCACCGGCAAAATCAACCGCGGCCGGATCGCCGAAATCAACGGCATCAGCGTCCGGGCCTTCTACCAGTGGTGGTCGATCACCGGAATCAGGTCCAGCGGCGAGAATTTGGTGTTTGAGGGTTCTACTTTGCTCGGGTCTCCATCAATTAGATTATGTGATGGTGTCCAGTTTTTAATTCCAATACTAATGCTACTTAAGCAAGTTTCTGGAGTTCAAAGTCTCTCTTCCAGTGTGCAAAGCACTCCCGAGAAGAATGGGCATTCAGATGATGCTTCAAGAAGTCCAGAGCTCCTTCAACAGTTTCTGAAATCTGGTCCCAGGAAGGAACTTCTTCGGGCATGTGTTGACAAGGACAAGAAAAATTTGACTTCCTCAAAAAACAAGATGGCCGAACTTTTGAAGACTACTAACAAGACAAATAAGAAGCAGGAGTCAAAAAAAGCTTCTTCTAGTCCAAATAATCATCCTCCTAAAAAGCAATCCAGAAAGGGGGAAAATCCCATCCGACTCTCACCAGCTTCTGAGCAGTCTCCAGATTTTGGATGTTCGAACTCATGGATCTGTAAAAACTCTGCTTGTAGAGCTGTTCTATCCATAGACAACACATTCTGCAAGAGATGCTCCTGCTGTATTTGTCATTTGTTTGATGACAACAAGGACCCAAGTCTTTGGTTAGTATGCACATCTGAATCTGGTGAGGGAGATTCCTGTGGGTTATCTTGTCACATTGACTGCGCGCTTAGATGTGAAAAGGTGGGAGTTGTTGATCTTGGGCAATTGATGCAGCTAGATGGTAGTTATTGTTGTGCTTCATGTGGTAAAGTTTCTGGGATACTTGGGATTTGGAAGAAGCAGCTACTCGTAGCAAAGGATGCTCGACGTGTTGATGTTCTTTGGGACAGGATATGCTTGAGTTATAGGCTTCTGGATGGGACTTCAAGGTTTAAAGAACTTCATGATATTGTGAAGGAAGCGAAGACTAAACTCGAGACAGAACTAGGTCCAATTAATGGAGATTCAGGAACAATGGTACGTGGGATAGTCAGCAGACTATCTATCGCTGGTGATGTGCTAAAACTCTGCTCTCTTGCAATTGAGAAAGCTGATGAATGGTTGGCCAATGTTTCTAGTGCAGACCCAAACAGAGAGGGTTCACTTCCAGCTGCCTGCAAGTTTCTCTTTGAGGAAGTAACACCTTCATCTGTTGTGATTATATTGATCGAACTGTCTAATGCTTCATCTGATGACATCAAGGGATACAAGCTCTGGTATTACAAGAGCAGAGAAGAATCACACACAAAAGAGCCTACTTGTACCTTTTCAAGATCTCAGAGAAGAATTTTGATCTCCAATCTGCAGCCTTGCACAGAGTATACATTTAGGATAATATCTTATACTGAGGCTGGTGACTTGGGTCACTCTGAGGCAAAGTGTTTCACCAAAAGTGTTGAGATAATTCACAAAAACTCGGATTCACCGGTCTGCAGGAATCATAAGAAAGAAAATCCTGTCATTGAGGCAAATTCTAGTGCCATGAGGGAGTCTGAAACTACAACAGCAGTTGTGCCTTCTTCCGAATTTAAAGTTCGTGATCTTGGAAAGATACTTCGTCTGGCTCAGGCTCAACAGAAAGGCTCCTTGGAGGGCTTTTGTAGTGCCAACAAAGAAAAATGTTGTGGACCAAGCAACCCGATAAAAACTGAAACTCTACAAGAAGAGTTGCCTTCTGTTCCACGGGGGCTTGACTTAAATGTTCTTTCAATGCCCGATCTGAATGAAGAGCTAACCCCGCCCTTTGAGTCCTCCAGAGATGAAGATAATGGATGCACTTTGCAGCAGGCTGTGGAGGCAGATGATGATGCTGCTTCTCATGACATGGTGAAGAACGGCCTAGCAAGATCACATGGTAGTGGTGATTCACAGACCTGGACTCATGGGATTACTGGAGATGTCCCTGCTGTTGATTCTCGGGCAGAAATTTGCAGGAAGAGGAAAGGAAACTCAAACGAAGAGACCCATGATTGTGACAGCACCCTGATAAATGGGCCACCATTGCAAAATGGTTCTTGTTGCTTGGACGAGAACTTTGAGTACTGTGTGAAAATAATCAGGTGGCTGGAATGCGAGGGTCACCTTACGGAGGAATTCAGATTGAAGTTGCTAACATGGTTCAGTTTGAGATCAACTGAGCAAGAGCGTAGGGTGGTCAACACCTTTATTCAAACTATGATTGATGATCCAAGTAGCTTGGCAGGACAGTTGGTTGACTCCTTTGCAGATATCATATCCCGCAAGAGGCAACGGAACGGATTCTGCAGTAAGCTGTGGCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

868

Amino Acids

96.02

Weight (kDa)

7.14

Isoelectric Point (pI)

54.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF538	PF04398	37 - 131	1.3e-21	Protein of unknown function, DUF538
PHD_Oberon	PF07227	279 - 397	1.4e-31	PHD - plant homeodomain finger protein
Fn3_VIN3	PF23376	484 - 571	4.2e-32	VIN3-like, fibronectin type-III domain
fn3	PF00041	485 - 565	1.2e-06	Fibronectin type III domain
VIN3_C	PF23380	784 - 857	1.9e-39	VIN3-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	200
AccB7I	CCANNNNNTGG	2 cut(s)	812, 1283
AccI	GTMKAC	2 cut(s)	204, 1646
AccII	CGCG	3 cut(s)	218, 291, 1010
AciI	CCGC	8 cut(s)	63, 124, 236, 289, 291, 369, 2063, 2563
AclWI	GGATC	3 cut(s)	305, 839, 2504
AcoI	YGGCCR	4 cut(s)	252, 292, 660, 1389
AcsI	RAATTY	7 cut(s)	376, 634, 1611, 1795, 1850, 2250, 2417
AfaI	GTAC	3 cut(s)	1305, 1588, 2366
AfiI	CCNNNNNNNGG	9 cut(s)	103, 157, 188, 360, 762, 812, 1283, 2413, 2568
AflII	CTTAAG	1 cut(s)	467
AflIII	ACRYGT	2 cut(s)	216, 1153
AgeI	ACCGGT	1 cut(s)	1750
AgsI	TTSAA	9 cut(s)	488, 549, 569, 673, 1212, 1596, 2034, 2428, 2495
AjiI	CACGTC	1 cut(s)	1154
AjnI	CCWGG	2 cut(s)	590, 2198
AjuI	GAANNNNNNNTTGG	2 cut(s)	1895, 1927
Alw21I	GWGCWC	1 cut(s)	564
Alw26I	GTCTC	5 cut(s)	414, 497, 807, 1256, 2279
AlwI	GGATC	3 cut(s)	305, 839, 2504
AlwNI	CAGNNNCTG	2 cut(s)	577, 794
Ama87I	CYCGRG	4 cut(s)	404, 518, 1259, 2241
AoxI	GGCC	8 cut(s)	105, 252, 292, 327, 660, 1389, 2161, 2318
ApeKI	GCWGC	6 cut(s)	1057, 1126, 1436, 1630, 2107, 2135
ApoI	RAATTY	7 cut(s)	376, 634, 1611, 1795, 1850, 2250, 2417
ArsI	GACNNNNNNTTYG	2 cut(s)	2342, 2374
AseI	ATTAAT	1 cut(s)	1281
AsiGI	ACCGGT	1 cut(s)	1750
Asp700I	GAANNNNTTC	2 cut(s)	603, 2582
AspLEI	GCGC	2 cut(s)	1010, 1012
AspS9I	GGNCC	8 cut(s)	106, 327, 363, 587, 931, 1274, 1952, 2318
AsuC2I	CCSGG	1 cut(s)	326
AsuII	TTCGAA	1 cut(s)	821
AvaI	CYCGRG	4 cut(s)	404, 518, 1259, 2241
AvaII	GGWCC	5 cut(s)	363, 587, 931, 1274, 1952
BalI	TGGCCA	2 cut(s)	254, 1391
BanII	GRGCYC	2 cut(s)	147, 564
BbsI	GAAGAC	2 cut(s)	680, 1256
Bbv12I	GWGCWC	1 cut(s)	564
BbvI	GCAGC	6 cut(s)	1069, 1138, 1423, 1642, 2119, 2122
BccI	CCATC	7 cut(s)	106, 421, 425, 652, 782, 1058, 1196
BceAI	ACGGC	3 cut(s)	145, 328, 2176
BciT130I	CCWGG	2 cut(s)	592, 2200
BciVI	GTATCC	2 cut(s)	1098, 1523
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	5 cut(s)	414, 497, 807, 1256, 2279
BfaI	CTAG	6 cut(s)	723, 1061, 1271, 1401, 1800, 2165
BfmI	CTRYAG	3 cut(s)	1628, 1756, 2587
BfrI	CTTAAG	1 cut(s)	467
BfuI	GTATCC	2 cut(s)	1098, 1523
BglII	AGATCT	1 cut(s)	1598
BisI	GCNGC	8 cut(s)	292, 370, 1058, 1127, 1437, 1631, 2108, 2136
BlsI	GCNGC	8 cut(s)	293, 371, 1059, 1128, 1438, 1632, 2109, 2137
BmcAI	AGTACT	1 cut(s)	2366
Bme1390I	CCNGG	3 cut(s)	326, 592, 2200
Bme18I	GGWCC	5 cut(s)	363, 587, 931, 1274, 1952
BmeT110I	CYCGRG	4 cut(s)	404, 518, 1259, 2241
BmgBI	CACGTC	1 cut(s)	1154
BmgT120I	GGNCC	8 cut(s)	106, 327, 363, 587, 931, 1274, 1952, 2318
BmiI	GGNNCC	4 cut(s)	146, 589, 933, 1909
BmrFI	CCNGG	3 cut(s)	326, 592, 2200
BmsI	GCATC	7 cut(s)	324, 532, 881, 1044, 1135, 2087, 2122
BoxI	GACNNNNGTC	2 cut(s)	936, 1686
BpiI	GAAGAC	2 cut(s)	680, 1256
BpmI	CTGGAG	4 cut(s)	501, 789, 2062, 2238
Bpu10I	CCTNAGC	1 cut(s)	1890
Bpu14I	TTCGAA	1 cut(s)	821
BpuEI	CTTGAG	1 cut(s)	1204
BpuMI	CCSGG	1 cut(s)	326
BsaAI	YACGTR	2 cut(s)	258, 1307
BsaI	GGTCTC	2 cut(s)	414, 2279
BsaJI	CCNNGG	5 cut(s)	208, 289, 590, 1911, 2006
BsaWI	WCCGGW	3 cut(s)	157, 353, 1750
Bsc4I	CCNNNNNNNGG	9 cut(s)	103, 157, 188, 360, 762, 812, 1283, 2413, 2568
Bse118I	RCCGGY	4 cut(s)	103, 188, 275, 1750
Bse1I	ACTGG	4 cut(s)	337, 439, 501, 2221
Bse3DI	GCAATG	1 cut(s)	2324
BseBI	CCWGG	2 cut(s)	592, 2200
BseDI	CCNNGG	5 cut(s)	208, 289, 590, 1911, 2006
BseGI	GGATG	7 cut(s)	33, 736, 774, 821, 1150, 1207, 2102
BseLI	CCNNNNNNNGG	9 cut(s)	103, 157, 188, 360, 762, 812, 1283, 2413, 2568
BseMI	GCAATG	1 cut(s)	2324
BseMII	CTCAG	6 cut(s)	786, 1616, 1662, 1686, 1904, 2451
BseNI	ACTGG	4 cut(s)	337, 439, 501, 2221
BsePI	GCGCGC	1 cut(s)	1008
BseRI	GAGGAG	4 cut(s)	5, 29, 43, 137
BseX3I	CGGCCG	1 cut(s)	292
BseXI	GCAGC	6 cut(s)	1069, 1138, 1423, 1642, 2119, 2122
BsgI	GTGCAG	1 cut(s)	1425
Bsh1236I	CGCG	3 cut(s)	218, 291, 1010
Bsh1285I	CGRYCG	1 cut(s)	295
BshFI	GGCC	8 cut(s)	107, 254, 294, 329, 662, 1391, 2163, 2320
BshTI	ACCGGT	1 cut(s)	1750
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	1 cut(s)	564
BsiHKCI	CYCGRG	4 cut(s)	404, 518, 1259, 2241
BsiSI	CCGG	9 cut(s)	104, 158, 189, 239, 276, 295, 325, 354, 1751
BslFI	GGGAC	4 cut(s)	573, 1184, 1219, 2210
BslI	CCNNNNNNNGG	9 cut(s)	103, 157, 188, 360, 762, 812, 1283, 2413, 2568
BsmAI	GTCTC	5 cut(s)	414, 497, 807, 1256, 2279
BsmFI	GGGAC	4 cut(s)	573, 1184, 1219, 2210
BsmI	GAATGC	2 cut(s)	532, 2399
BsnI	GGCC	8 cut(s)	107, 254, 294, 329, 662, 1391, 2163, 2320
Bso31I	GGTCTC	2 cut(s)	414, 2279
BsoBI	CYCGRG	4 cut(s)	404, 518, 1259, 2241
Bsp119I	TTCGAA	1 cut(s)	821
Bsp1286I	GDGCHC	2 cut(s)	147, 564
BspACI	CCGC	8 cut(s)	63, 124, 236, 289, 291, 369, 2063, 2563
BspANI	GGCC	8 cut(s)	107, 254, 294, 329, 662, 1391, 2163, 2320
BspCNI	CTCAG	6 cut(s)	787, 1615, 1663, 1687, 1903, 2452
BspFNI	CGCG	3 cut(s)	218, 291, 1010
BspHI	TCATGA	2 cut(s)	1228, 2143
BspLI	GGNNCC	4 cut(s)	146, 589, 933, 1909
BspMAI	CTGCAG	3 cut(s)	1632, 1760, 2591
BspPI	GGATC	3 cut(s)	305, 839, 2504
BspQI	GCTCTTC	1 cut(s)	2046
BspT104I	TTCGAA	1 cut(s)	821
BspTI	CTTAAG	1 cut(s)	467
BspTNI	GGTCTC	2 cut(s)	414, 2279
BsrDI	GCAATG	1 cut(s)	2324
BsrFI	RCCGGY	4 cut(s)	103, 188, 275, 1750
BsrI	ACTGG	4 cut(s)	337, 439, 501, 2221
BssAI	RCCGGY	4 cut(s)	103, 188, 275, 1750
BssECI	CCNNGG	5 cut(s)	208, 289, 590, 1911, 2006
BssHII	GCGCGC	1 cut(s)	1008
BssNAI	GTATAC	1 cut(s)	1647
BssT1I	CCWWGG	1 cut(s)	1911
Bst1107I	GTATAC	1 cut(s)	1647
Bst2UI	CCWGG	2 cut(s)	592, 2200
Bst4CI	ACNGT	5 cut(s)	175, 573, 1500, 2369, 2532
Bst6I	CTCTTC	6 cut(s)	23, 502, 1983, 2046, 2255, 2277
BstAFI	CTTAAG	1 cut(s)	467
BstAPI	GCANNNNNTGC	3 cut(s)	891, 1054, 1145
BstBAI	YACGTR	2 cut(s)	258, 1307
BstBI	TTCGAA	1 cut(s)	821
BstC8I	GCNNGC	5 cut(s)	105, 220, 1010, 1441, 2112
BstDEI	CTNAG	7 cut(s)	795, 1013, 1602, 1671, 1695, 1890, 2460
BstDSI	CCRYGG	2 cut(s)	289, 2006
BstEII	GGTNACC	1 cut(s)	2402
BstENI	CCTNNNNNAGG	1 cut(s)	2411
BstF5I	GGATG	7 cut(s)	33, 736, 774, 821, 1150, 1207, 2102
BstFNI	CGCG	3 cut(s)	218, 291, 1010
BstHHI	GCGC	2 cut(s)	1010, 1012
BstMAI	GTCTC	5 cut(s)	414, 497, 807, 1256, 2279
BstMCI	CGRYCG	1 cut(s)	295
BstMWI	GCNNNNNNNGC	9 cut(s)	219, 469, 854, 891, 1054, 1145, 2062, 2120, 2326
BstNI	CCWGG	2 cut(s)	592, 2200
BstNSI	RCATGY	1 cut(s)	615
BstPAI	GACNNNNGTC	2 cut(s)	936, 1686
BstPI	GGTNACC	1 cut(s)	2402
BstSCI	CCNGG	3 cut(s)	324, 590, 2198
BstSFI	CTRYAG	3 cut(s)	1628, 1756, 2587
BstUI	CGCG	3 cut(s)	218, 291, 1010
BstV1I	GCAGC	6 cut(s)	1069, 1138, 1423, 1642, 2119, 2122
BstV2I	GAAGAC	2 cut(s)	680, 1256
BstX2I	RGATCY	2 cut(s)	831, 1598
BstXI	CCANNNNNNTGG	1 cut(s)	942
BstYI	RGATCY	2 cut(s)	831, 1598
BstZ17I	GTATAC	1 cut(s)	1647
BstZI	CGGCCG	1 cut(s)	292
BsuI	GTATCC	2 cut(s)	1098, 1523
BsuRI	GGCC	8 cut(s)	107, 254, 294, 329, 662, 1391, 2163, 2320
BtgI	CCRYGG	2 cut(s)	289, 2006
BtgZI	GCGATG	1 cut(s)	84
BtrI	CACGTC	1 cut(s)	1154
BtsCI	GGATG	7 cut(s)	33, 736, 774, 821, 1150, 1207, 2102
BtsIMutI	CAGTG	2 cut(s)	344, 508
Cac8I	GCNNGC	5 cut(s)	105, 220, 1010, 1441, 2112
CaiI	CAGNNNCTG	2 cut(s)	577, 794
CciI	TCATGA	2 cut(s)	1228, 2143
CfoI	GCGC	2 cut(s)	1010, 1012
Cfr10I	RCCGGY	4 cut(s)	103, 188, 275, 1750
Cfr13I	GGNCC	8 cut(s)	106, 327, 363, 587, 931, 1274, 1952, 2318
Cfr42I	CCGCGG	1 cut(s)	292
CseI	GACGC	2 cut(s)	224, 310
Csp6I	GTAC	3 cut(s)	1304, 1587, 2365
CspAI	ACCGGT	1 cut(s)	1750
CviQI	GTAC	3 cut(s)	1304, 1587, 2365
DdeI	CTNAG	7 cut(s)	795, 1013, 1602, 1671, 1695, 1890, 2460
DraI	TTTAAA	2 cut(s)	1219, 1855
DrdI	GACNNNNNNGTC	1 cut(s)	200
DseDI	GACNNNNNNGTC	1 cut(s)	200
EaeI	YGGCCR	4 cut(s)	252, 292, 660, 1389
EagI	CGGCCG	1 cut(s)	292
Eam1104I	CTCTTC	6 cut(s)	23, 502, 1983, 2046, 2255, 2277
EarI	CTCTTC	6 cut(s)	23, 502, 1983, 2046, 2255, 2277
Ecl136II	GAGCTC	1 cut(s)	562
EclXI	CGGCCG	1 cut(s)	292
Eco130I	CCWWGG	1 cut(s)	1911
Eco24I	GRGCYC	2 cut(s)	147, 564
Eco31I	GGTCTC	2 cut(s)	414, 2279
Eco32I	GATATC	1 cut(s)	2554
Eco47I	GGWCC	5 cut(s)	363, 587, 931, 1274, 1952
Eco52I	CGGCCG	1 cut(s)	292
Eco53kI	GAGCTC	1 cut(s)	562
Eco88I	CYCGRG	4 cut(s)	404, 518, 1259, 2241
Eco91I	GGTNACC	1 cut(s)	2402
EcoICRI	GAGCTC	1 cut(s)	562
EcoNI	CCTNNNNNAGG	1 cut(s)	2411
EcoO109I	RGGNCCY	1 cut(s)	931
EcoO65I	GGTNACC	1 cut(s)	2402
EcoRI	GAATTC	1 cut(s)	2417
EcoRII	CCWGG	2 cut(s)	590, 2198
EcoRV	GATATC	1 cut(s)	2554
EcoT14I	CCWWGG	1 cut(s)	1911
EcoT38I	GRGCYC	2 cut(s)	147, 564
ErhI	CCWWGG	1 cut(s)	1911
FalI	AAGNNNNNCTT	4 cut(s)	481, 513, 1436, 1468
FaqI	GGGAC	4 cut(s)	573, 1184, 1219, 2210
FauI	CCCGC	4 cut(s)	131, 243, 2070, 2570
FblI	GTMKAC	2 cut(s)	204, 1646
Fnu4HI	GCNGC	8 cut(s)	292, 370, 1058, 1127, 1437, 1631, 2108, 2136
FokI	GGATG	7 cut(s)	20, 723, 761, 828, 1157, 1214, 2109
FriOI	GRGCYC	2 cut(s)	147, 564
Fsp4HI	GCNGC	8 cut(s)	292, 370, 1058, 1127, 1437, 1631, 2108, 2136
FspBI	CTAG	6 cut(s)	723, 1061, 1271, 1401, 1800, 2165
GlaI	GCGC	2 cut(s)	1009, 1011
GluI	GCNGC	8 cut(s)	292, 370, 1058, 1127, 1437, 1631, 2108, 2136
GsuI	CTGGAG	4 cut(s)	501, 789, 2062, 2238
HaeIII	GGCC	8 cut(s)	107, 254, 294, 329, 662, 1391, 2163, 2320
HapII	CCGG	9 cut(s)	104, 158, 189, 239, 276, 295, 325, 354, 1751
HgaI	GACGC	2 cut(s)	224, 310
HhaI	GCGC	2 cut(s)	1010, 1012
Hin6I	GCGC	2 cut(s)	1008, 1010
HinP1I	GCGC	2 cut(s)	1008, 1010
HincII	GTYRAC	5 cut(s)	178, 205, 619, 2482, 2539
HindII	GTYRAC	5 cut(s)	178, 205, 619, 2482, 2539
HindIII	AAGCTT	1 cut(s)	714
HpaII	CCGG	9 cut(s)	104, 158, 189, 239, 276, 295, 325, 354, 1751
Hpy166II	GTNNAC	8 cut(s)	178, 205, 619, 1427, 1647, 1952, 2482, 2539
Hpy8I	GTNNAC	8 cut(s)	178, 205, 619, 1427, 1647, 1952, 2482, 2539
Hpy99I	CGWCG	4 cut(s)	203, 206, 218, 1155
HpyAV	CCTTC	8 cut(s)	162, 340, 575, 589, 1234, 1479, 1851, 2007
HpyCH4III	ACNGT	5 cut(s)	175, 573, 1500, 2369, 2532
HpyCH4IV	ACGT	3 cut(s)	257, 1153, 1306
HpyF10VI	GCNNNNNNNGC	9 cut(s)	219, 469, 854, 891, 1054, 1145, 2062, 2120, 2326
HpyF3I	CTNAG	7 cut(s)	795, 1013, 1602, 1671, 1695, 1890, 2460
HpySE526I	ACGT	3 cut(s)	257, 1153, 1306
HspAI	GCGC	2 cut(s)	1008, 1010
KroI	GCCGGC	1 cut(s)	103
KroNI	GCCGGC	1 cut(s)	105
KspI	CCGCGG	1 cut(s)	292
LguI	GCTCTTC	1 cut(s)	2046
LmnI	GCTCC	5 cut(s)	150, 237, 567, 899, 1913
Lsp1109I	GCAGC	6 cut(s)	1069, 1138, 1423, 1642, 2119, 2122
LweI	GCATC	7 cut(s)	324, 532, 881, 1044, 1135, 2087, 2122
MaeI	CTAG	6 cut(s)	723, 1061, 1271, 1401, 1800, 2165
MaeII	ACGT	3 cut(s)	257, 1153, 1306
MaeIII	GTNAC	7 cut(s)	271, 995, 1462, 1679, 1688, 2297, 2402
MfeI	CAATTG	2 cut(s)	1049, 1365
MflI	RGATCY	2 cut(s)	831, 1598
MhlI	GDGCHC	2 cut(s)	147, 564
MlsI	TGGCCA	2 cut(s)	254, 1391
MluI	ACGCGT	1 cut(s)	216
MluNI	TGGCCA	2 cut(s)	254, 1391
MlyI	GAGTC	6 cut(s)	713, 774, 1823, 2081, 2197, 2534
MmeI	TCCRAC	1 cut(s)	802
Mox20I	TGGCCA	2 cut(s)	254, 1391
MroNI	GCCGGC	1 cut(s)	103
MroXI	GAANNNNTTC	2 cut(s)	603, 2582
MscI	TGGCCA	2 cut(s)	254, 1391
MseI	TTAA	7 cut(s)	446, 468, 1218, 1281, 1854, 2021, 2605
MslI	CAYNNNNRTG	3 cut(s)	537, 2181, 2295
Msp20I	TGGCCA	2 cut(s)	254, 1391
MspA1I	CMGCKG	3 cut(s)	291, 369, 1436
MspCI	CTTAAG	1 cut(s)	467
MspI	CCGG	9 cut(s)	104, 158, 189, 239, 276, 295, 325, 354, 1751
MspR9I	CCNGG	3 cut(s)	326, 592, 2200
MunI	CAATTG	2 cut(s)	1049, 1365
Mva1269I	GAATGC	2 cut(s)	532, 2399
MvaI	CCWGG	2 cut(s)	592, 2200
MvnI	CGCG	3 cut(s)	218, 291, 1010
MwoI	GCNNNNNNNGC	9 cut(s)	219, 469, 854, 891, 1054, 1145, 2062, 2120, 2326
NaeI	GCCGGC	1 cut(s)	105
NciI	CCSGG	1 cut(s)	326
NgoMIV	GCCGGC	1 cut(s)	103
NlaIV	GGNNCC	4 cut(s)	146, 589, 933, 1909
NmeAIII	GCCGAG	1 cut(s)	189
NmuCI	GTSAC	6 cut(s)	271, 995, 1679, 1688, 2297, 2402
NspI	RCATGY	1 cut(s)	615
NspV	TTCGAA	1 cut(s)	821
PaeR7I	CTCGAG	1 cut(s)	1259
PagI	TCATGA	2 cut(s)	1228, 2143
PauI	GCGCGC	1 cut(s)	1008
PciSI	GCTCTTC	1 cut(s)	2046
PcsI	WCGNNNNNNNCGW	2 cut(s)	210, 318
PctI	GAATGC	2 cut(s)	532, 2399
PdiI	GCCGGC	1 cut(s)	105
PdmI	GAANNNNTTC	2 cut(s)	603, 2582
PflFI	GACNNNGTC	1 cut(s)	197
PflMI	CCANNNNNTGG	2 cut(s)	812, 1283
PinAI	ACCGGT	1 cut(s)	1750
PkrI	GCNGC	8 cut(s)	293, 371, 1059, 1128, 1438, 1632, 2109, 2137
PleI	GAGTC	6 cut(s)	712, 774, 1822, 2080, 2197, 2534
PpsI	GAGTC	6 cut(s)	712, 774, 1822, 2080, 2197, 2534
Ppu21I	YACGTR	2 cut(s)	258, 1307
PpuMI	RGGWCCY	1 cut(s)	931
PshAI	GACNNNNGTC	2 cut(s)	936, 1686
PshBI	ATTAAT	1 cut(s)	1281
Psp124BI	GAGCTC	1 cut(s)	564
Psp5II	RGGWCCY	1 cut(s)	931
Psp6I	CCWGG	2 cut(s)	590, 2198
PspEI	GGTNACC	1 cut(s)	2402
PspGI	CCWGG	2 cut(s)	590, 2198
PspN4I	GGNNCC	4 cut(s)	146, 589, 933, 1909
PspPI	GGNCC	8 cut(s)	106, 327, 363, 587, 931, 1274, 1952, 2318
PspPPI	RGGWCCY	1 cut(s)	931
PsrI	GAACNNNNNNTAC	2 cut(s)	2348, 2380
PstI	CTGCAG	3 cut(s)	1632, 1760, 2591
PstNI	CAGNNNCTG	2 cut(s)	577, 794
PsuI	RGATCY	2 cut(s)	831, 1598
PsyI	GACNNNGTC	1 cut(s)	197
PteI	GCGCGC	1 cut(s)	1008
PvuII	CAGCTG	1 cut(s)	1436
RsaI	GTAC	3 cut(s)	1305, 1588, 2366
RsaNI	GTAC	3 cut(s)	1304, 1587, 2365
RseI	CAYNNNNRTG	3 cut(s)	537, 2181, 2295
SacI	GAGCTC	1 cut(s)	564
SacII	CCGCGG	1 cut(s)	292
SalI	GTCGAC	1 cut(s)	203
SapI	GCTCTTC	1 cut(s)	2046
SaqAI	TTAA	7 cut(s)	446, 468, 1218, 1281, 1854, 2021, 2605
SatI	GCNGC	8 cut(s)	292, 370, 1058, 1127, 1437, 1631, 2108, 2136
Sau96I	GGNCC	8 cut(s)	106, 327, 363, 587, 931, 1274, 1952, 2318
ScaI	AGTACT	1 cut(s)	2366
SchI	GAGTC	6 cut(s)	713, 774, 1823, 2081, 2197, 2534
ScrFI	CCNGG	3 cut(s)	326, 592, 2200
SduI	GDGCHC	2 cut(s)	147, 564
SfaNI	GCATC	7 cut(s)	324, 532, 881, 1044, 1135, 2087, 2122
SfcI	CTRYAG	3 cut(s)	1628, 1756, 2587
Sfr274I	CTCGAG	1 cut(s)	1259
Sfr303I	CCGCGG	1 cut(s)	292
SfuI	TTCGAA	1 cut(s)	821
SgrAI	CRCCGGYG	1 cut(s)	188
SgrBI	CCGCGG	1 cut(s)	292
SinI	GGWCC	5 cut(s)	363, 587, 931, 1274, 1952
SlaI	CTCGAG	1 cut(s)	1259
SmiMI	CAYNNNNRTG	3 cut(s)	537, 2181, 2295
SmlI	CTYRAG	3 cut(s)	467, 1183, 1259
SmoI	CTYRAG	3 cut(s)	467, 1183, 1259
SsiI	CCGC	8 cut(s)	63, 124, 236, 289, 291, 369, 2063, 2563
SspMI	CTAG	6 cut(s)	723, 1061, 1271, 1401, 1800, 2165
SstI	GAGCTC	1 cut(s)	564
StyD4I	CCNGG	3 cut(s)	324, 590, 2198
StyI	CCWWGG	1 cut(s)	1911
TaaI	ACNGT	5 cut(s)	175, 573, 1500, 2369, 2532
TaiI	ACGT	3 cut(s)	260, 1156, 1309
TaqI	TCGA	6 cut(s)	204, 347, 821, 1150, 1260, 1494
TatI	WGTACW	1 cut(s)	2364
TauI	GCSGC	2 cut(s)	294, 372
Tru1I	TTAA	7 cut(s)	446, 468, 1218, 1281, 1854, 2021, 2605
Tru9I	TTAA	7 cut(s)	446, 468, 1218, 1281, 1854, 2021, 2605
TscAI	CASTG	2 cut(s)	344, 508
TseFI	GTSAC	6 cut(s)	271, 995, 1679, 1688, 2297, 2402
TseI	GCWGC	6 cut(s)	1057, 1126, 1436, 1630, 2107, 2135
Tsp45I	GTSAC	6 cut(s)	271, 995, 1679, 1688, 2297, 2402
TspDTI	ATGAA	7 cut(s)	1074, 1217, 1395, 1461, 1500, 2064, 2100
TspGWI	ACGGA	3 cut(s)	2426, 2590, 2595
TspRI	CASTG	2 cut(s)	344, 508
Tth111I	GACNNNGTC	1 cut(s)	197
Van91I	CCANNNNNTGG	2 cut(s)	812, 1283
Vha464I	CTTAAG	1 cut(s)	467
VpaK11BI	GGWCC	5 cut(s)	363, 587, 931, 1274, 1952
VspI	ATTAAT	1 cut(s)	1281
XagI	CCTNNNNNAGG	1 cut(s)	2411
XapI	RAATTY	7 cut(s)	376, 634, 1611, 1795, 1850, 2250, 2417
XceI	RCATGY	1 cut(s)	615
XhoI	CTCGAG	1 cut(s)	1259
XmiI	GTMKAC	2 cut(s)	204, 1646
XmnI	GAANNNNTTC	2 cut(s)	603, 2582
XspI	CTAG	6 cut(s)	723, 1061, 1271, 1401, 1800, 2165
ZrmI	AGTACT	1 cut(s)	2366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.