Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Reverse (-)

18156590 .. 18162358

Gene Structure 5769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1223500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGCGGAACGTGTGCTGACCCGCGTCCAGAGTCTCCGGGAGAGGCTGGACGAAACCCTTTCTGCTCACCGCAATGAGATTCTGGCCCTTCTTTCAAGGATTGAGAGCAAGGGGAAAGGGTTTCTGCAACCCCATCAGCTTCTTGCCGAGTTCGAAGAGATTCCTGAAGCGAACAGGCAGAAGCTGTTGGATGGGGCTTTTGGTGAAGTTTTGAAGAACACCCAGGAGGTAATTGTTCTGCCCCCATGGGTGGCGCTTGCTGTCCGCCCAAGGCCTGGTGTTTGGGAGTACATTCGTGTGAATGTCCATGCTCTCGTTGTCGAGGAGTTACGTGTGGCTGAGTACTTGCAATTCAAGGAGGAACTTGTTGATGGAAGTGCCAATGGCAACTTTGTGCTCGAGTTGGACTTTGAGCCATTCAACGCCTCCTTCCCCCGCCCAACTCTTTCCAAGTCCATTGGAAACGGCGTTGAGTTCCTCAACCGCCACCTTTCTGCAAAACTCTTCCATGACAAGGAGAGCCTGCACCCGCTGCTCAAGTTTCTCCGTGTTCACTGCTACGAGGGCAGGAACCTGATGCTGAATAACAAAATTAAGGATGTGAATGAGCTGCAACATGTTCTGAGGAAGGCAGAGGATTTCCTATCTGCAATTGCCCCTGAAACTCCGTACAAGGACTTCGAGCCCAAGTTGCAAGCCATTGGCTTGGAAAGGGGTTGGGGTGACACTGCTGGGCGTGTGCTCGAAATGATCGAACTTCTGTTGGACCTTCTTGAGGCACCCGATCCTTGCACTCTTGAGAAATTCCTTGACCAAATCCCCATGGTCTTTAATGTTGTGATCCTCTCCCCCCACGGCTATTTCGCCCAAGACAATGTCTTGGGGTACCCTGACACCGGTGGCCAGGTTGTCTATATCTTGGATCAAGTTCGCGCATTGGAGGCTGAGATGCTCAAGCGTGTTAAGCAACAGGGACTGGATATCATACCCCGCATTATCATTCTCACAAGGCTTCTTCCTGATGCCGTTGGAACCACATGTGGTGATCGACTTGAGAAAGTATACGGGACTGAGCATTCAGATATTCTTCGTGTTCCATTCAGAGATGAAAAAGGAGCCATTGTCCGCAGATGGATTTCAAGATTCGAAGTCTGGCCCTACCTAGAGACATACGCCGAGGATGCGATCAAGGAACTTTCCAAGGAAATGCATGGCAAGCCTGATCTCATCATCGGAAACTACAGTGATGGCAACATTGTGGCCTCACTGATGGCACTCAAATTGGGTGTTACACAGTGCACCATTGCTCATGCTCTCGAGAAGTCAAAATACCCTGATTCTGACCTTTACTGGAAGAAATTGGATGACAGTTATCACTTCTCATGCCAATTTACAGCTGATCTTATCGCCATGAACCACACTGATTTCATCATCACCAGTACCTATCAAGAGATTGCTGGAAGCAAAGAAACTGTTGGTCAGTATGAGAGCCACACTGCTTTTACTCTTCCTGGGCTCTACCGTGTTGTTCATGGTATTGATGTCTTCGATCCCAAGTTCAACATTGTTTCACCAGGAGCTGACATGAGCGTCTACTTCCCTTACTCTGAGAAAGAAAAGAGGCTGACTAACTTCCACCCTGAAATCGAAGAGCTCCTTTACAGCCAAGTCGAAAATAAGGAACACTTATGTGTGTTGAAGGACAGAAACAAGCCAATCATCTTCACAATGGCAAGGCTGGACCGCGTGAAGAACATCACTGGGCTTGTCGAGTGGTATGGTAAGAATGCCAAGCTCAGAGAGTTGGTTAACCTGGTTGTAGTTGCTGGTGATCGCCGCAAGGAGTCCAAGGACAATGAGGAGAAGGCTGAGATGAAGAAGATGTATGAGCTTATTGACACCTACAAGCTGAACGGGCAGTTCAGATGGATTTCTTCCCAGATGAACCGTGTCAGGAATGGTGAACTTTACCGCTACATTTGTGACACCAAGGGAGCCTTTGTGCAGCCTGCTGTGTATGAAGCTTTCGGCTTGACTGTCGTTGAGGCCATGACTTGTGGACTGCCCACATTTGCCACTTGCAAGGGTGGCCCGGCTGAAATTATAGTGCATGGCAAATCTGGCTACCACATTGATCCATATCACGGCGATCAAGCCGCTGAGATCCTTGTTGATTTCTTTGAGAAGAGCAAGGCAGATCCTAGCCACTGGGACAAGATTTCTCAAGGCGGCTTGCAGCGCATTTATGAGAAGTACACATGGCAAATCTACTCTCAGAGGCTCCTGACCCTCACCGGAGTTTATGGTTTCTGGAAGCATGTTTCAAACCTTGATCGCCTGGAGAGCCGCCGCTATCTGGAGATGTTTTACGCCCTCAAGTACAGCAAGCTGGCGGCGTCTGTTCCTCTTGCTGTGGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

808

Amino Acids

92.37

Weight (kDa)

5.88

Isoelectric Point (pI)

32.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	9 - 120	1.2e-46	Sucrose synthase N-terminal
SUS_EPBD	PF24862	155 - 242	1.8e-33	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	265 - 554	2.9e-164	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	567 - 730	8.5e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	572 - 724	4e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1589
Acc65I	GGTACC	1 cut(s)	885
AccB1I	GGYRCC	2 cut(s)	778, 885
AccB7I	CCANNNNNTGG	1 cut(s)	275
AccI	GTMKAC	2 cut(s)	1062, 1593
AccII	CGCG	3 cut(s)	24, 933, 1746
AclWI	GGATC	7 cut(s)	779, 835, 930, 1544, 2129, 2158, 2192
AcoI	YGGCCR	1 cut(s)	901
AcsI	RAATTY	1 cut(s)	803
AcuI	CTGAAG	1 cut(s)	186
AcyI	GRCGYC	1 cut(s)	2396
AfaI	GTAC	7 cut(s)	290, 344, 671, 887, 1441, 2255, 2381
AfiI	CCNNNNNNNGG	7 cut(s)	250, 275, 514, 775, 896, 2144, 2356
AflIII	ACRYGT	4 cut(s)	10, 331, 616, 1037
AgeI	ACCGGT	1 cut(s)	896
AgsI	TTSAA	8 cut(s)	96, 214, 355, 421, 1140, 1561, 1699, 2325
AjnI	CCWGG	7 cut(s)	222, 274, 903, 1510, 1573, 1812, 2337
AleI	CACNNNNGTG	1 cut(s)	1689
Alw21I	GWGCWC	4 cut(s)	399, 744, 1301, 1656
Alw26I	GTCTC	2 cut(s)	38, 1160
Alw44I	GTGCAC	1 cut(s)	1297
AlwI	GGATC	7 cut(s)	779, 835, 930, 1544, 2129, 2158, 2192
AlwNI	CAGNNNCTG	3 cut(s)	184, 976, 1580
Ama87I	CYCGRG	2 cut(s)	398, 1316
AoxI	GGCC	7 cut(s)	84, 272, 901, 1154, 1260, 2046, 2089
ApaLI	GTGCAC	1 cut(s)	1297
ApeKI	GCWGC	4 cut(s)	532, 610, 2005, 2236
ApoI	RAATTY	1 cut(s)	803
AsiGI	ACCGGT	1 cut(s)	896
Asp700I	GAANNNNTTC	1 cut(s)	159
Asp718I	GGTACC	1 cut(s)	885
AspLEI	GCGC	3 cut(s)	256, 935, 2241
AspS9I	GGNCC	5 cut(s)	85, 766, 1155, 1741, 2090
AsuC2I	CCSGG	2 cut(s)	38, 2093
AsuHPI	GGTGA	9 cut(s)	59, 215, 734, 1055, 1426, 1563, 1841, 1973, 2284
AsuII	TTCGAA	2 cut(s)	153, 1146
AvaI	CYCGRG	2 cut(s)	398, 1316
AvaII	GGWCC	2 cut(s)	766, 1741
BaeGI	GKGCMC	1 cut(s)	1301
BalI	TGGCCA	1 cut(s)	903
BanI	GGYRCC	2 cut(s)	778, 885
BanII	GRGCYC	3 cut(s)	687, 1518, 1656
BarI	GAAGNNNNNNTAC	4 cut(s)	662, 694, 1313, 1345
BbsI	GAAGAC	1 cut(s)	1537
Bbv12I	GWGCWC	4 cut(s)	399, 744, 1301, 1656
BbvI	GCAGC	4 cut(s)	519, 597, 2017, 2248
BccI	CCATC	7 cut(s)	141, 185, 365, 1125, 1241, 1264, 1920
BceAI	ACGGC	4 cut(s)	481, 871, 1010, 2161
BciT130I	CCWGG	7 cut(s)	224, 276, 905, 1512, 1575, 1814, 2339
BcnI	CCSGG	2 cut(s)	38, 2093
BcoDI	GTCTC	2 cut(s)	38, 1160
BfaI	CTAG	2 cut(s)	1163, 2202
BfmI	CTRYAG	1 cut(s)	1240
BfoI	RGCGCY	1 cut(s)	257
BmcAI	AGTACT	1 cut(s)	344
Bme1390I	CCNGG	9 cut(s)	38, 224, 276, 905, 1512, 1575, 1814, 2093, 2339
Bme18I	GGWCC	2 cut(s)	766, 1741
BmeT110I	CYCGRG	2 cut(s)	398, 1316
BmgT120I	GGNCC	5 cut(s)	85, 766, 1155, 1741, 2090
BmiI	GGNNCC	7 cut(s)	572, 780, 887, 1033, 1117, 1996, 2282
BmrFI	CCNGG	9 cut(s)	38, 224, 276, 905, 1512, 1575, 1814, 2093, 2339
BmrI	ACTGGG	2 cut(s)	1770, 2218
BmsI	GCATC	4 cut(s)	567, 939, 1012, 1171
BmuI	ACTGGG	2 cut(s)	1770, 2218
BoxI	GACNNNNGTC	1 cut(s)	23
BpiI	GAAGAC	1 cut(s)	1537
BpmI	CTGGAG	2 cut(s)	2360, 2378
Bpu14I	TTCGAA	2 cut(s)	153, 1146
BpuEI	CTTGAG	7 cut(s)	521, 794, 818, 938, 1073, 2208, 2360
BpuMI	CCSGG	2 cut(s)	38, 2093
BsaAI	YACGTR	1 cut(s)	332
BsaBI	GATNNNNATC	1 cut(s)	2139
BsaHI	GRCGYC	1 cut(s)	2396
BsaWI	WCCGGW	2 cut(s)	896, 2294
BsaXI	ACNNNNNCTCC	4 cut(s)	1107, 1137, 1986, 2016
Bsc4I	CCNNNNNNNGG	7 cut(s)	250, 275, 514, 775, 896, 2144, 2356
Bse118I	RCCGGY	1 cut(s)	896
Bse1I	ACTGG	5 cut(s)	981, 1355, 1437, 1765, 2213
Bse3DI	GCAATG	2 cut(s)	79, 1302
Bse8I	GATNNNNATC	1 cut(s)	2139
BseBI	CCWGG	7 cut(s)	224, 276, 905, 1512, 1575, 1814, 2339
BseGI	GGATG	4 cut(s)	196, 604, 1186, 1369
BseJI	GATNNNNATC	1 cut(s)	2139
BseLI	CCNNNNNNNGG	7 cut(s)	250, 275, 514, 775, 896, 2144, 2356
BseMI	GCAATG	2 cut(s)	79, 1302
BseMII	CTCAG	9 cut(s)	330, 614, 936, 1062, 1599, 1810, 1860, 2151, 2288
BseNI	ACTGG	5 cut(s)	981, 1355, 1437, 1765, 2213
BseRI	GAGGAG	2 cut(s)	338, 1874
BseSI	GKGCMC	1 cut(s)	1301
BseXI	GCAGC	4 cut(s)	519, 597, 2017, 2248
BseYI	CCCAGC	1 cut(s)	731
BsgI	GTGCAG	2 cut(s)	509, 2024
Bsh1236I	CGCG	3 cut(s)	24, 933, 1746
BshFI	GGCC	7 cut(s)	86, 274, 903, 1156, 1262, 2048, 2091
BshNI	GGYRCC	2 cut(s)	778, 885
BshTI	ACCGGT	1 cut(s)	896
BsiHKAI	GWGCWC	4 cut(s)	399, 744, 1301, 1656
BsiHKCI	CYCGRG	2 cut(s)	398, 1316
BsiSI	CCGG	4 cut(s)	37, 897, 2093, 2295
BslFI	GGGAC	3 cut(s)	987, 1081, 2225
BslI	CCNNNNNNNGG	7 cut(s)	250, 275, 514, 775, 896, 2144, 2356
BsmAI	GTCTC	2 cut(s)	38, 1160
BsmFI	GGGAC	3 cut(s)	987, 1081, 2225
BsmI	GAATGC	2 cut(s)	1075, 1792
BsnI	GGCC	7 cut(s)	86, 274, 903, 1156, 1262, 2048, 2091
BsoBI	CYCGRG	2 cut(s)	398, 1316
Bsp119I	TTCGAA	2 cut(s)	153, 1146
Bsp1286I	GDGCHC	6 cut(s)	399, 687, 744, 1301, 1518, 1656
Bsp19I	CCATGG	2 cut(s)	245, 822
BspANI	GGCC	7 cut(s)	86, 274, 903, 1156, 1262, 2048, 2091
BspCNI	CTCAG	9 cut(s)	331, 615, 937, 1063, 1600, 1809, 1861, 2152, 2287
BspFNI	CGCG	3 cut(s)	24, 933, 1746
BspLI	GGNNCC	7 cut(s)	572, 780, 887, 1033, 1117, 1996, 2282
BspPI	GGATC	7 cut(s)	779, 835, 930, 1544, 2129, 2158, 2192
BspQI	GCTCTTC	2 cut(s)	1644, 2180
BspT104I	TTCGAA	2 cut(s)	153, 1146
BspT107I	GGYRCC	2 cut(s)	778, 885
BsrDI	GCAATG	2 cut(s)	79, 1302
BsrFI	RCCGGY	1 cut(s)	896
BsrI	ACTGG	5 cut(s)	981, 1355, 1437, 1765, 2213
BssAI	RCCGGY	1 cut(s)	896
BssNAI	GTATAC	1 cut(s)	1063
BssNI	GRCGYC	1 cut(s)	2396
BssT1I	CCWWGG	6 cut(s)	245, 269, 822, 1200, 1848, 1989
Bst1107I	GTATAC	1 cut(s)	1063
Bst2UI	CCWGG	7 cut(s)	224, 276, 905, 1512, 1575, 1814, 2339
Bst4CI	ACNGT	7 cut(s)	1244, 1296, 1370, 1474, 1523, 1949, 2038
Bst6I	CTCTTC	5 cut(s)	150, 509, 1512, 1644, 2180
BstACI	GRCGYC	1 cut(s)	2396
BstAPI	GCANNNNNTGC	1 cut(s)	532
BstBAI	YACGTR	1 cut(s)	332
BstBI	TTCGAA	2 cut(s)	153, 1146
BstC8I	GCNNGC	8 cut(s)	258, 524, 696, 1217, 2010, 2234, 2387, 2391
BstDEI	CTNAG	9 cut(s)	339, 623, 945, 1071, 1608, 1796, 1869, 2160, 2274
BstDSI	CCRYGG	3 cut(s)	245, 822, 853
BstENI	CCTNNNNNAGG	1 cut(s)	773
BstF5I	GGATG	4 cut(s)	196, 604, 1186, 1369
BstFNI	CGCG	3 cut(s)	24, 933, 1746
BstH2I	RGCGCY	1 cut(s)	257
BstHHI	GCGC	3 cut(s)	256, 935, 2241
BstMAI	GTCTC	2 cut(s)	38, 1160
BstNI	CCWGG	7 cut(s)	224, 276, 905, 1512, 1575, 1814, 2339
BstNSI	RCATGY	3 cut(s)	620, 1041, 2321
BstPAI	GACNNNNGTC	1 cut(s)	23
BstSCI	CCNGG	9 cut(s)	36, 222, 274, 903, 1510, 1573, 1812, 2091, 2337
BstSFI	CTRYAG	1 cut(s)	1240
BstSLI	GKGCMC	1 cut(s)	1301
BstUI	CGCG	3 cut(s)	24, 933, 1746
BstV1I	GCAGC	4 cut(s)	519, 597, 2017, 2248
BstV2I	GAAGAC	1 cut(s)	1537
BstX2I	RGATCY	2 cut(s)	2163, 2197
BstXI	CCANNNNNNTGG	1 cut(s)	706
BstYI	RGATCY	2 cut(s)	2163, 2197
BstZ17I	GTATAC	1 cut(s)	1063
BsuRI	GGCC	7 cut(s)	86, 274, 903, 1156, 1262, 2048, 2091
BtgI	CCRYGG	3 cut(s)	245, 822, 853
BtsCI	GGATG	4 cut(s)	196, 604, 1186, 1369
BtsI	GCAGTG	3 cut(s)	553, 726, 1494
BtsIMutI	CAGTG	9 cut(s)	553, 726, 1249, 1265, 1301, 1419, 1494, 1758, 2206
Cac8I	GCNNGC	8 cut(s)	258, 524, 696, 1217, 2010, 2234, 2387, 2391
CaiI	CAGNNNCTG	3 cut(s)	184, 976, 1580
CfoI	GCGC	3 cut(s)	256, 935, 2241
Cfr10I	RCCGGY	1 cut(s)	896
Cfr13I	GGNCC	5 cut(s)	85, 766, 1155, 1741, 2090
CseI	GACGC	3 cut(s)	13, 1579, 2385
CsiI	ACCWGGT	1 cut(s)	1812
Csp6I	GTAC	7 cut(s)	289, 343, 670, 886, 1440, 2254, 2380
CspAI	ACCGGT	1 cut(s)	896
CviQI	GTAC	7 cut(s)	289, 343, 670, 886, 1440, 2254, 2380
DdeI	CTNAG	9 cut(s)	339, 623, 945, 1071, 1608, 1796, 1869, 2160, 2274
DrdI	GACNNNNNNGTC	1 cut(s)	1589
DseDI	GACNNNNNNGTC	1 cut(s)	1589
EaeI	YGGCCR	1 cut(s)	901
Eam1104I	CTCTTC	5 cut(s)	150, 509, 1512, 1644, 2180
EarI	CTCTTC	5 cut(s)	150, 509, 1512, 1644, 2180
EciI	GGCGGA	2 cut(s)	20, 254
Ecl136II	GAGCTC	1 cut(s)	1654
Eco130I	CCWWGG	6 cut(s)	245, 269, 822, 1200, 1848, 1989
Eco147I	AGGCCT	1 cut(s)	274
Eco24I	GRGCYC	3 cut(s)	687, 1518, 1656
Eco32I	GATATC	1 cut(s)	982
Eco47I	GGWCC	2 cut(s)	766, 1741
Eco53kI	GAGCTC	1 cut(s)	1654
Eco57I	CTGAAG	1 cut(s)	186
Eco88I	CYCGRG	2 cut(s)	398, 1316
EcoICRI	GAGCTC	1 cut(s)	1654
EcoNI	CCTNNNNNAGG	1 cut(s)	773
EcoRII	CCWGG	7 cut(s)	222, 274, 903, 1510, 1573, 1812, 2337
EcoRV	GATATC	1 cut(s)	982
EcoT14I	CCWWGG	6 cut(s)	245, 269, 822, 1200, 1848, 1989
EcoT22I	ATGCAT	1 cut(s)	1212
EcoT38I	GRGCYC	3 cut(s)	687, 1518, 1656
ErhI	CCWWGG	6 cut(s)	245, 269, 822, 1200, 1848, 1989
FalI	AAGNNNNNCTT	6 cut(s)	1641, 1673, 1670, 1702, 1982, 2014
FaqI	GGGAC	3 cut(s)	987, 1081, 2225
FauI	CCCGC	4 cut(s)	29, 443, 537, 998
FblI	GTMKAC	2 cut(s)	1062, 1593
FokI	GGATG	4 cut(s)	203, 611, 1193, 1376
FriOI	GRGCYC	3 cut(s)	687, 1518, 1656
FspBI	CTAG	2 cut(s)	1163, 2202
GlaI	GCGC	3 cut(s)	255, 934, 2240
GsaI	CCCAGC	1 cut(s)	735
GsuI	CTGGAG	2 cut(s)	2360, 2378
HaeII	RGCGCY	1 cut(s)	257
HaeIII	GGCC	7 cut(s)	86, 274, 903, 1156, 1262, 2048, 2091
HapII	CCGG	4 cut(s)	37, 897, 2093, 2295
HgaI	GACGC	3 cut(s)	13, 1579, 2385
HhaI	GCGC	3 cut(s)	256, 935, 2241
Hin1I	GRCGYC	1 cut(s)	2396
Hin6I	GCGC	3 cut(s)	254, 933, 2239
HinP1I	GCGC	3 cut(s)	254, 933, 2239
HincII	GTYRAC	1 cut(s)	1810
HindII	GTYRAC	1 cut(s)	1810
HindIII	AAGCTT	1 cut(s)	2022
HinfI	GANTC	6 cut(s)	31, 79, 160, 1143, 1337, 1844
HpaI	GTTAAC	1 cut(s)	1810
HpaII	CCGG	4 cut(s)	37, 897, 2093, 2295
HphI	GGTGA	9 cut(s)	59, 215, 734, 1055, 1426, 1563, 1841, 1973, 2284
Hpy166II	GTNNAC	8 cut(s)	553, 1063, 1299, 1594, 1810, 1964, 2060, 2256
Hpy188I	TCNGA	9 cut(s)	624, 1081, 1103, 1235, 1342, 1609, 1799, 1925, 2277
Hpy8I	GTNNAC	8 cut(s)	553, 1063, 1299, 1594, 1810, 1964, 2060, 2256
HpyAV	CCTTC	6 cut(s)	98, 439, 622, 779, 1693, 1858
HpyCH4III	ACNGT	7 cut(s)	1244, 1296, 1370, 1474, 1523, 1949, 2038
HpyCH4IV	ACGT	2 cut(s)	10, 331
HpyF3I	CTNAG	9 cut(s)	339, 623, 945, 1071, 1608, 1796, 1869, 2160, 2274
HpySE526I	ACGT	2 cut(s)	10, 331
Hsp92I	GRCGYC	1 cut(s)	2396
HspAI	GCGC	3 cut(s)	254, 933, 2239
KpnI	GGTACC	1 cut(s)	889
KspAI	GTTAAC	1 cut(s)	1810
LguI	GCTCTTC	2 cut(s)	1644, 2180
LmnI	GCTCC	5 cut(s)	1115, 1577, 1659, 1994, 2286
Lsp1109I	GCAGC	4 cut(s)	519, 597, 2017, 2248
LweI	GCATC	4 cut(s)	567, 939, 1012, 1171
MabI	ACCWGGT	1 cut(s)	1812
MaeI	CTAG	2 cut(s)	1163, 2202
MaeII	ACGT	2 cut(s)	10, 331
MaeIII	GTNAC	4 cut(s)	327, 722, 1288, 1982
MfeI	CAATTG	1 cut(s)	651
MflI	RGATCY	2 cut(s)	2163, 2197
MhlI	GDGCHC	6 cut(s)	399, 687, 744, 1301, 1518, 1656
MlsI	TGGCCA	1 cut(s)	903
MluCI	AATT	9 cut(s)	231, 350, 591, 651, 803, 1280, 1358, 1388, 2100
MluNI	TGGCCA	1 cut(s)	903
MlyI	GAGTC	2 cut(s)	40, 1853
MmeI	TCCRAC	4 cut(s)	168, 384, 744, 1009
Mox20I	TGGCCA	1 cut(s)	903
Mph1103I	ATGCAT	1 cut(s)	1212
MroXI	GAANNNNTTC	1 cut(s)	159
MscI	TGGCCA	1 cut(s)	903
MseI	TTAA	4 cut(s)	594, 831, 963, 1809
MslI	CAYNNNNRTG	3 cut(s)	72, 296, 1689
Msp20I	TGGCCA	1 cut(s)	903
MspA1I	CMGCKG	3 cut(s)	532, 1397, 2159
MspI	CCGG	4 cut(s)	37, 897, 2093, 2295
MspR9I	CCNGG	9 cut(s)	38, 224, 276, 905, 1512, 1575, 1814, 2093, 2339
MunI	CAATTG	1 cut(s)	651
Mva1269I	GAATGC	2 cut(s)	1075, 1792
MvaI	CCWGG	7 cut(s)	224, 276, 905, 1512, 1575, 1814, 2339
MvnI	CGCG	3 cut(s)	24, 933, 1746
NciI	CCSGG	2 cut(s)	38, 2093
NcoI	CCATGG	2 cut(s)	245, 822
NlaIV	GGNNCC	7 cut(s)	572, 780, 887, 1033, 1117, 1996, 2282
NmeAIII	GCCGAG	2 cut(s)	172, 1201
NmuCI	GTSAC	2 cut(s)	722, 1982
NsiI	ATGCAT	1 cut(s)	1212
NspI	RCATGY	3 cut(s)	620, 1041, 2321
NspV	TTCGAA	2 cut(s)	153, 1146
OliI	CACNNNNGTG	1 cut(s)	1689
PaeR7I	CTCGAG	2 cut(s)	398, 1316
PceI	AGGCCT	1 cut(s)	274
PciI	ACATGT	2 cut(s)	616, 1037
PciSI	GCTCTTC	2 cut(s)	1644, 2180
PcsI	WCGNNNNNNNCGW	1 cut(s)	750
PctI	GAATGC	2 cut(s)	1075, 1792
PdmI	GAANNNNTTC	1 cut(s)	159
PfeI	GAWTC	4 cut(s)	79, 160, 1143, 1337
PflFI	GACNNNGTC	1 cut(s)	875
PflMI	CCANNNNNTGG	1 cut(s)	275
PfoI	TCCNGGA	1 cut(s)	36
PinAI	ACCGGT	1 cut(s)	896
PleI	GAGTC	2 cut(s)	39, 1852
PpsI	GAGTC	2 cut(s)	39, 1852
Ppu21I	YACGTR	1 cut(s)	332
PscI	ACATGT	2 cut(s)	616, 1037
PshAI	GACNNNNGTC	1 cut(s)	23
Psp124BI	GAGCTC	1 cut(s)	1656
Psp6I	CCWGG	7 cut(s)	222, 274, 903, 1510, 1573, 1812, 2337
PspFI	CCCAGC	1 cut(s)	731
PspGI	CCWGG	7 cut(s)	222, 274, 903, 1510, 1573, 1812, 2337
PspN4I	GGNNCC	7 cut(s)	572, 780, 887, 1033, 1117, 1996, 2282
PspPI	GGNCC	5 cut(s)	85, 766, 1155, 1741, 2090
PspXI	VCTCGAGB	1 cut(s)	398
PstNI	CAGNNNCTG	3 cut(s)	184, 976, 1580
PsuI	RGATCY	2 cut(s)	2163, 2197
PsyI	GACNNNGTC	1 cut(s)	875
PvuII	CAGCTG	1 cut(s)	1397
RsaI	GTAC	7 cut(s)	290, 344, 671, 887, 1441, 2255, 2381
RsaNI	GTAC	7 cut(s)	289, 343, 670, 886, 1440, 2254, 2380
RseI	CAYNNNNRTG	3 cut(s)	72, 296, 1689
SacI	GAGCTC	1 cut(s)	1656
SapI	GCTCTTC	2 cut(s)	1644, 2180
SaqAI	TTAA	4 cut(s)	594, 831, 963, 1809
Sau96I	GGNCC	5 cut(s)	85, 766, 1155, 1741, 2090
ScaI	AGTACT	1 cut(s)	344
SchI	GAGTC	2 cut(s)	40, 1853
ScrFI	CCNGG	9 cut(s)	38, 224, 276, 905, 1512, 1575, 1814, 2093, 2339
SduI	GDGCHC	6 cut(s)	399, 687, 744, 1301, 1518, 1656
SexAI	ACCWGGT	1 cut(s)	1812
SfaNI	GCATC	4 cut(s)	567, 939, 1012, 1171
SfcI	CTRYAG	1 cut(s)	1240
Sfr274I	CTCGAG	2 cut(s)	398, 1316
SfuI	TTCGAA	2 cut(s)	153, 1146
SgrAI	CRCCGGYG	1 cut(s)	896
SinI	GGWCC	2 cut(s)	766, 1741
SlaI	CTCGAG	2 cut(s)	398, 1316
SmiMI	CAYNNNNRTG	3 cut(s)	72, 296, 1689
SmlI	CTYRAG	9 cut(s)	398, 536, 773, 797, 953, 1052, 1316, 2223, 2375
SmoI	CTYRAG	9 cut(s)	398, 536, 773, 797, 953, 1052, 1316, 2223, 2375
Sse9I	AATT	9 cut(s)	231, 350, 591, 651, 803, 1280, 1358, 1388, 2100
SseBI	AGGCCT	1 cut(s)	274
SspMI	CTAG	2 cut(s)	1163, 2202
SstI	GAGCTC	1 cut(s)	1656
StuI	AGGCCT	1 cut(s)	274
StyD4I	CCNGG	9 cut(s)	36, 222, 274, 903, 1510, 1573, 1812, 2091, 2337
StyI	CCWWGG	6 cut(s)	245, 269, 822, 1200, 1848, 1989
TaaI	ACNGT	7 cut(s)	1244, 1296, 1370, 1474, 1523, 1949, 2038
TaiI	ACGT	2 cut(s)	13, 334
TasI	AATT	9 cut(s)	231, 350, 591, 651, 803, 1280, 1358, 1388, 2100
TatI	WGTACW	4 cut(s)	288, 342, 2253, 2379
TauI	GCSGC	6 cut(s)	1839, 2159, 2232, 2349, 2352, 2396
TfiI	GAWTC	4 cut(s)	79, 160, 1143, 1337
Tru1I	TTAA	4 cut(s)	594, 831, 963, 1809
Tru9I	TTAA	4 cut(s)	594, 831, 963, 1809
TscAI	CASTG	9 cut(s)	560, 733, 1249, 1272, 1301, 1426, 1501, 1765, 2213
TseFI	GTSAC	2 cut(s)	722, 1982
TseI	GCWGC	4 cut(s)	532, 610, 2005, 2236
Tsp45I	GTSAC	2 cut(s)	722, 1982
TspDTI	ATGAA	7 cut(s)	1122, 1417, 1427, 1520, 1889, 1958, 2034
TspGWI	ACGGA	2 cut(s)	536, 657
TspRI	CASTG	9 cut(s)	560, 733, 1249, 1272, 1301, 1426, 1501, 1765, 2213
Tth111I	GACNNNGTC	1 cut(s)	875
Van91I	CCANNNNNTGG	1 cut(s)	275
VneI	GTGCAC	1 cut(s)	1297
VpaK11BI	GGWCC	2 cut(s)	766, 1741
XagI	CCTNNNNNAGG	1 cut(s)	773
XapI	RAATTY	1 cut(s)	803
XceI	RCATGY	3 cut(s)	620, 1041, 2321
XhoI	CTCGAG	2 cut(s)	398, 1316
XmiI	GTMKAC	2 cut(s)	1062, 1593
XmnI	GAANNNNTTC	1 cut(s)	159
XspI	CTAG	2 cut(s)	1163, 2202
ZrmI	AGTACT	1 cut(s)	344
Zsp2I	ATGCAT	1 cut(s)	1212

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.