Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Reverse (-)

5022657 .. 5028170

Gene Structure 5514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g09360.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGCGGACTCACGTGCTCTGAACCGTGCCCATAGCCTCAGGGAGAGGCTGGATGACACTCTCTCTGCTCACCGTAACGAGATCCTAGCCTTTCTGTCAAGGATTGAAGCCAAGGGAAAAGGGTTTTTGCAGCCCCATCAGCTGATTGATGAGTTTGAAGCAATTCCTGAAGCCAGCAGAAAAAATTTGTTGGAAAGTGAATTTGGGGAAGTTCTGAAGAACACCCAGGAAGCTATTGTTCTTCCTCCATGGGTGGCACTGGCTGTTCGTCCCAGGCCTGGTGTTTGGGAGTACATTCGGGTCAATGTGCACTCACTTGTTGCCGAGGAGTTGCTTGTGCCCGAGTACTTGCACTTCAAAGAGGAGCTTGTTGATGGAAGTCTTAATGGCAACTTTGTGCTTGAATTGGACTTTGAACCATTCAATGCCACTTTCCCTAAGCCAACTCTTTCCAAATCTATTGGAAATGGTGTCGAGTTCCTCAACCGTCACCTCTCTGCAAAGCTCTTCCATGACAAGGAGAGCATGCACCCTCTGCTCGAGTTTCTCCGAGTTCACTGCTACAAGGGCAAGAATATGATGCTCAATGGCAAAATTCAGAATGTGAATGCACTTCAACATAATCTGAGAAAGGCCGAGGAGTATCTGTCTAAGCTCGCACCAGAAACCCCATACAAAACTTTTGAGATCAAGTTGCAGGATCTTGGTTTGGAGAGAGGTTGGGGAGACAACGCTGAGCGCGTGCTGGAAATGATCCAACTCCTATTGGAACTCCTTGAGGCACCTGACCCTTGCAATCTGGAAAAATTCCTTGGCAGAATTCCCATGGTGTTCAATGTTGTGATCATGTCTCCTCATGGTTACTTTGCCCAAGACAATGTGTTGGGGTACCCTGACACTGGTGGCCAGGTTGTTTATATCTTGGATCAAGTTCGTGCTTTGGAGAATGAGATGCTTAAACGTATCAAGGAACAAGGGCTTGACATTATCCCTCGCATTCTTATTGTCACAAGGCTCCTTCCTGATGCAGTAGGAACTACTTGTGGTCAGCGTCTTGAGAAGGTTTACAACACCAAACACTCCGATATTCTTCGTGTTCCCTTTAGAACCGAGAAGGGAATTGTCCGCAAATGGATCTCAAGATTTGAAGTGTGGCCATACCTAGAGACTTACACCGAGGATGTTGCAACAGAACTTGCTAAAGAGTTCCAGGGCAAGCCTGATCTCATTATTGGAAACTACAGTGATGGTAACATTGTGGCCTCTTTGTTGGCTCATAAATTGAGTGTTACTCAATGCACCATTGCCCATGCTTTGGAGAAGACCAAGTACCCTGATTCTGACCTTTACTGGAAGAAACTAGATGACAAGTACCATTTCTCTTGCCAGTTCACAGCTGATCTTATCGCCATGAACCACACCGACTTCATCATCACCAGTACCTTCCAGGAGATTGCCGGAAGCAAAGATACCGTTGGCCAGTACGAGAGTCACACCGCTTTCACCATGCCCGGTCTCTACCGTGTTGTTCATGGGATTGATGTCTTTGATCCCAAGTTCAACATTGTTTCACCAGGAGCTGACATGGACATTTACTTCCCTTATTCCGAGAAGGAAAAGAGGTTGACACATTTCCACCCGGAAATTGAAGAGCTCATCTACAGCCAAGAGGAGAACAAAGAACACTTGTGTGTGTTGAAGGACAGGAACAAGCCAATCATCTTCACAATGGCAAGGCTGGACCGTGTAAAGAACATCACTGGGCTGGTTGAGTGGTATGGTAAGAACAAGAAGCTTAGAGAGCTGGTTAACTTGGTTGTAGTTGCTGGGGATCGACGAAAGGAGTCCCAGGACAATGAGGAGAAAGCTGAGCTGAAGAAGATGTATGAGCTGATTGACACCTACAAGCTGAATGGGCAGTTCAGATGGATTTCCTCTCAGATGAACCGCGTGAGGAACGGTGAACTCTACCGTGTCATTTGTGACACCAAGGGAGCTTTTGTGCAGCCTGCTGTGTATGAAGCCTTTGGCTTGACTGTTGTTGAGGCCATGACCTGTGGTTTGCCCACATTTGCTACTTGCAAAGGTGGCCCAGCAGAGATTATTGTGCATGGCAAATCTGGGTACCATATTGATCCATACCATGGTGAGCAAGCTGCTGAGATCCTTGTTGACTTCTTTGAGAAGTGCAAGAAAGACCCTTCTCACTGGGACAAGATTTCCCAGGGAGGCCTGAAGCGTATCGAGGAGAAGTACACATGGCAAATCTACTCTGAAAGGCTCCTGACCTTGACTGGAGTTTATGGGTTCTGGAAGCATGTCTCTAACCTTGATCGCCTTGAGAGTCGTCGCTACATCGAAATGTTTTATGCCCTCAAGTACAAAAAGCTGGCTGATTCTGTTCCTCTTGCTGAGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

807

Amino Acids

92.75

Weight (kDa)

6.1

Isoelectric Point (pI)

32.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	9 - 121	1.1e-45	Sucrose synthase N-terminal
SUS_EPBD	PF24862	156 - 243	4.1e-34	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	266 - 554	2.7e-170	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	567 - 739	1.6e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	572 - 725	4.5e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	888, 2124
AccB1I	GGYRCC	3 cut(s)	781, 888, 2124
AccB7I	CCANNNNNTGG	2 cut(s)	1315, 2144
AccII	CGCG	2 cut(s)	741, 1950
AciI	CCGC	4 cut(s)	5, 1126, 1497, 1948
AclWI	GGATC	9 cut(s)	76, 708, 748, 933, 1142, 1544, 1839, 2129, 2158
AcoI	YGGCCR	3 cut(s)	904, 1154, 1477
AcsI	RAATTY	5 cut(s)	184, 200, 594, 806, 819
AcuI	CTGAAG	4 cut(s)	189, 236, 1895, 2255
AcvI	CACGTG	1 cut(s)	14
AfiI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
AjnI	CCWGG	9 cut(s)	225, 272, 277, 906, 1209, 1446, 1573, 1848, 2223
AjuI	GAANNNNNNNTTGG	4 cut(s)	186, 218, 795, 827
AleI	CACNNNNGTG	1 cut(s)	1689
Alw21I	GWGCWC	3 cut(s)	19, 312, 1656
Alw26I	GTCTC	5 cut(s)	720, 855, 1160, 1520, 2326
Alw44I	GTGCAC	1 cut(s)	308
AlwI	GGATC	9 cut(s)	76, 708, 748, 933, 1142, 1544, 1839, 2129, 2158
AlwNI	CAGNNNCTG	1 cut(s)	1580
Ama87I	CYCGRG	2 cut(s)	341, 539
AoxI	GGCC	9 cut(s)	275, 633, 904, 1154, 1260, 1477, 2046, 2089, 2230
ApaLI	GTGCAC	1 cut(s)	308
ApeKI	GCWGC	3 cut(s)	130, 2005, 2156
ApoI	RAATTY	5 cut(s)	184, 200, 594, 806, 819
ArsI	GACNNNNNNTTYG	2 cut(s)	2044, 2076
Asp700I	GAANNNNTTC	1 cut(s)	162
Asp718I	GGTACC	2 cut(s)	888, 2124
AspLEI	GCGC	1 cut(s)	741
AspS9I	GGNCC	2 cut(s)	1741, 2090
AsuC2I	CCSGG	2 cut(s)	1512, 1640
AsuHPI	GGTGA	7 cut(s)	62, 482, 1426, 1495, 1563, 1973, 2159
AvaI	CYCGRG	2 cut(s)	341, 539
AvaII	GGWCC	1 cut(s)	1741
AxyI	CCTNAGG	1 cut(s)	38
BaeGI	GKGCMC	3 cut(s)	31, 312, 342
BalI	TGGCCA	3 cut(s)	906, 1156, 1479
BanI	GGYRCC	3 cut(s)	781, 888, 2124
BanII	GRGCYC	1 cut(s)	1656
BarI	GAAGNNNNNNTAC	6 cut(s)	338, 370, 1313, 1345, 1453, 1485
BbrPI	CACGTG	1 cut(s)	14
BbsI	GAAGAC	1 cut(s)	1328
Bbv12I	GWGCWC	3 cut(s)	19, 312, 1656
BbvCI	CCTCAGC	1 cut(s)	2412
BbvI	GCAGC	3 cut(s)	142, 2017, 2143
BccI	CCATC	4 cut(s)	144, 368, 1241, 1920
BcgI	CGANNNNNNTGC	4 cut(s)	540, 574, 1166, 1200
BciT130I	CCWGG	9 cut(s)	227, 274, 279, 908, 1211, 1448, 1575, 1850, 2225
BclI	TGATCA	1 cut(s)	843
BcnI	CCSGG	2 cut(s)	1512, 1640
BcoDI	GTCTC	5 cut(s)	720, 855, 1160, 1520, 2326
BfaI	CTAG	3 cut(s)	86, 1163, 1361
BfmI	CTRYAG	2 cut(s)	1240, 1660
BisI	GCNGC	3 cut(s)	131, 2006, 2157
BlpI	GCTNAGC	2 cut(s)	735, 1869
BlsI	GCNGC	3 cut(s)	132, 2007, 2158
BmcAI	AGTACT	1 cut(s)	347
Bme18I	GGWCC	1 cut(s)	1741
BmeT110I	CYCGRG	2 cut(s)	341, 539
BmgT120I	GGNCC	2 cut(s)	1741, 2090
BmiI	GGNNCC	5 cut(s)	783, 890, 1016, 2126, 2282
BmrI	ACTGGG	2 cut(s)	1770, 2218
BmsI	GCATC	3 cut(s)	570, 942, 1015
BmuI	ACTGGG	2 cut(s)	1770, 2218
BpiI	GAAGAC	1 cut(s)	1328
BplI	GAGNNNNNCTC	2 cut(s)	1276, 1308
BpmI	CTGGAG	1 cut(s)	2316
Bpu10I	CCTNAGC	2 cut(s)	438, 2412
Bpu1102I	GCTNAGC	2 cut(s)	735, 1869
BpuEI	CTTGAG	5 cut(s)	797, 1076, 1123, 2360, 2360
BpuMI	CCSGG	2 cut(s)	1512, 1640
BsaAI	YACGTR	1 cut(s)	14
BsaI	GGTCTC	1 cut(s)	1520
BsaXI	ACNNNNNCTCC	4 cut(s)	632, 662, 1986, 2016
Bsc4I	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
Bse1I	ACTGG	9 cut(s)	264, 904, 1355, 1387, 1437, 1480, 1765, 2213, 2299
Bse21I	CCTNAGG	1 cut(s)	38
Bse3DI	GCAATG	1 cut(s)	1302
BseBI	CCWGG	9 cut(s)	227, 274, 279, 908, 1211, 1448, 1575, 1850, 2225
BseGI	GGATG	2 cut(s)	58, 1186
BseLI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
BseMI	GCAATG	1 cut(s)	1302
BseMII	CTCAG	7 cut(s)	52, 617, 726, 1860, 1952, 2151, 2403
BseNI	ACTGG	9 cut(s)	264, 904, 1355, 1387, 1437, 1480, 1765, 2213, 2299
BseRI	GAGGAG	7 cut(s)	341, 377, 653, 843, 1685, 1874, 2261
BseSI	GKGCMC	3 cut(s)	31, 312, 342
BseXI	GCAGC	3 cut(s)	142, 2017, 2143
BseYI	CCCAGC	2 cut(s)	1826, 2092
BsgI	GTGCAG	1 cut(s)	2024
Bsh1236I	CGCG	2 cut(s)	741, 1950
BshFI	GGCC	9 cut(s)	277, 635, 906, 1156, 1262, 1479, 2048, 2091, 2232
BshNI	GGYRCC	3 cut(s)	781, 888, 2124
BsiHKAI	GWGCWC	3 cut(s)	19, 312, 1656
BsiHKCI	CYCGRG	2 cut(s)	341, 539
BsiSI	CCGG	3 cut(s)	1458, 1512, 1640
BslFI	GGGAC	3 cut(s)	255, 1831, 2225
BslI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
BsmAI	GTCTC	5 cut(s)	720, 855, 1160, 1520, 2326
BsmFI	GGGAC	3 cut(s)	255, 1831, 2225
BsmI	GAATGC	2 cut(s)	613, 996
BsnI	GGCC	9 cut(s)	277, 635, 906, 1156, 1262, 1479, 2048, 2091, 2232
Bso31I	GGTCTC	1 cut(s)	1520
BsoBI	CYCGRG	2 cut(s)	341, 539
Bsp1286I	GDGCHC	5 cut(s)	19, 31, 312, 342, 1656
Bsp1720I	GCTNAGC	2 cut(s)	735, 1869
Bsp19I	CCATGG	3 cut(s)	248, 825, 2143
BspACI	CCGC	4 cut(s)	5, 1126, 1497, 1948
BspANI	GGCC	9 cut(s)	277, 635, 906, 1156, 1262, 1479, 2048, 2091, 2232
BspCNI	CTCAG	7 cut(s)	51, 618, 727, 1861, 1951, 2152, 2404
BspFNI	CGCG	2 cut(s)	741, 1950
BspLI	GGNNCC	5 cut(s)	783, 890, 1016, 2126, 2282
BspPI	GGATC	9 cut(s)	76, 708, 748, 933, 1142, 1544, 1839, 2129, 2158
BspQI	GCTCTTC	2 cut(s)	512, 1644
BspT107I	GGYRCC	3 cut(s)	781, 888, 2124
BspTNI	GGTCTC	1 cut(s)	1520
BsrDI	GCAATG	1 cut(s)	1302
BsrI	ACTGG	9 cut(s)	264, 904, 1355, 1387, 1437, 1480, 1765, 2213, 2299
BssT1I	CCWWGG	6 cut(s)	111, 248, 811, 825, 1989, 2143
Bst2UI	CCWGG	9 cut(s)	227, 274, 279, 908, 1211, 1448, 1575, 1850, 2225
Bst6I	CTCTTC	2 cut(s)	512, 1644
BstAPI	GCANNNNNTGC	1 cut(s)	535
BstBAI	YACGTR	1 cut(s)	14
BstC8I	GCNNGC	8 cut(s)	175, 527, 657, 743, 1217, 2010, 2154, 2391
BstDSI	CCRYGG	3 cut(s)	248, 825, 2143
BstF5I	GGATG	2 cut(s)	58, 1186
BstFNI	CGCG	2 cut(s)	741, 1950
BstHHI	GCGC	1 cut(s)	741
BstMAI	GTCTC	5 cut(s)	720, 855, 1160, 1520, 2326
BstMWI	GCNNNNNNNGC	6 cut(s)	139, 535, 567, 1801, 1915, 2088
BstNI	CCWGG	9 cut(s)	227, 274, 279, 908, 1211, 1448, 1575, 1850, 2225
BstNSI	RCATGY	2 cut(s)	529, 2321
BstSFI	CTRYAG	2 cut(s)	1240, 1660
BstSLI	GKGCMC	3 cut(s)	31, 312, 342
BstUI	CGCG	2 cut(s)	741, 1950
BstV1I	GCAGC	3 cut(s)	142, 2017, 2143
BstV2I	GAAGAC	1 cut(s)	1328
BstX2I	RGATCY	4 cut(s)	81, 700, 1134, 2163
BstYI	RGATCY	4 cut(s)	81, 700, 1134, 2163
Bsu36I	CCTNAGG	1 cut(s)	38
BsuRI	GGCC	9 cut(s)	277, 635, 906, 1156, 1262, 1479, 2048, 2091, 2232
BtgI	CCRYGG	3 cut(s)	248, 825, 2143
BtsCI	GGATG	2 cut(s)	58, 1186
BtsI	GCAGTG	1 cut(s)	556
BtsIMutI	CAGTG	6 cut(s)	257, 556, 897, 1249, 1758, 2206
Cac8I	GCNNGC	8 cut(s)	175, 527, 657, 743, 1217, 2010, 2154, 2391
CaiI	CAGNNNCTG	1 cut(s)	1580
CfoI	GCGC	1 cut(s)	741
Cfr13I	GGNCC	2 cut(s)	1741, 2090
CseI	GACGC	1 cut(s)	1040
EaeI	YGGCCR	3 cut(s)	904, 1154, 1477
Eam1104I	CTCTTC	2 cut(s)	512, 1644
EarI	CTCTTC	2 cut(s)	512, 1644
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	1654
Eco130I	CCWWGG	6 cut(s)	111, 248, 811, 825, 1989, 2143
Eco147I	AGGCCT	2 cut(s)	277, 2232
Eco24I	GRGCYC	1 cut(s)	1656
Eco31I	GGTCTC	1 cut(s)	1520
Eco47I	GGWCC	1 cut(s)	1741
Eco53kI	GAGCTC	1 cut(s)	1654
Eco57I	CTGAAG	4 cut(s)	189, 236, 1895, 2255
Eco72I	CACGTG	1 cut(s)	14
Eco81I	CCTNAGG	1 cut(s)	38
Eco88I	CYCGRG	2 cut(s)	341, 539
EcoICRI	GAGCTC	1 cut(s)	1654
EcoRI	GAATTC	1 cut(s)	819
EcoRII	CCWGG	9 cut(s)	225, 272, 277, 906, 1209, 1446, 1573, 1848, 2223
EcoT14I	CCWWGG	6 cut(s)	111, 248, 811, 825, 1989, 2143
EcoT38I	GRGCYC	1 cut(s)	1656
ErhI	CCWWGG	6 cut(s)	111, 248, 811, 825, 1989, 2143
FalI	AAGNNNNNCTT	2 cut(s)	351, 383
FaqI	GGGAC	3 cut(s)	255, 1831, 2225
FbaI	TGATCA	1 cut(s)	843
Fnu4HI	GCNGC	3 cut(s)	131, 2006, 2157
FokI	GGATG	2 cut(s)	65, 1193
FriOI	GRGCYC	1 cut(s)	1656
Fsp4HI	GCNGC	3 cut(s)	131, 2006, 2157
FspBI	CTAG	3 cut(s)	86, 1163, 1361
GlaI	GCGC	1 cut(s)	740
GluI	GCNGC	3 cut(s)	131, 2006, 2157
GsaI	CCCAGC	2 cut(s)	1830, 2096
GsuI	CTGGAG	1 cut(s)	2316
HaeIII	GGCC	9 cut(s)	277, 635, 906, 1156, 1262, 1479, 2048, 2091, 2232
HapII	CCGG	3 cut(s)	1458, 1512, 1640
HgaI	GACGC	1 cut(s)	1040
HhaI	GCGC	1 cut(s)	741
Hin6I	GCGC	1 cut(s)	739
HinP1I	GCGC	1 cut(s)	739
HincII	GTYRAC	3 cut(s)	1626, 1810, 2173
HindII	GTYRAC	3 cut(s)	1626, 1810, 2173
HindIII	AAGCTT	1 cut(s)	1793
HinfI	GANTC	6 cut(s)	8, 1337, 1489, 1844, 2344, 2396
HpaI	GTTAAC	1 cut(s)	1810
HpaII	CCGG	3 cut(s)	1458, 1512, 1640
HphI	GGTGA	7 cut(s)	62, 482, 1426, 1495, 1563, 1973, 2159
Hpy166II	GTNNAC	9 cut(s)	310, 556, 1066, 1392, 1626, 1810, 1964, 2173, 2256
Hpy188III	TCNNGA	7 cut(s)	167, 800, 1022, 1055, 1140, 2284, 2311
Hpy8I	GTNNAC	9 cut(s)	310, 556, 1066, 1392, 1626, 1810, 1964, 2173, 2256
Hpy99I	CGWCG	2 cut(s)	1839, 2352
HpyAV	CCTTC	7 cut(s)	1028, 1054, 1108, 1453, 1606, 1693, 2211
HpyCH4IV	ACGT	2 cut(s)	13, 961
HpyF10VI	GCNNNNNNNGC	6 cut(s)	139, 535, 567, 1801, 1915, 2088
HpySE526I	ACGT	2 cut(s)	13, 961
HspAI	GCGC	1 cut(s)	739
KpnI	GGTACC	2 cut(s)	892, 2128
Ksp22I	TGATCA	1 cut(s)	843
KspAI	GTTAAC	1 cut(s)	1810
LguI	GCTCTTC	2 cut(s)	512, 1644
LmnI	GCTCC	5 cut(s)	364, 1020, 1577, 1994, 2286
Lsp1109I	GCAGC	3 cut(s)	142, 2017, 2143
LweI	GCATC	3 cut(s)	570, 942, 1015
MaeI	CTAG	3 cut(s)	86, 1163, 1361
MaeII	ACGT	2 cut(s)	13, 961
MaeIII	GTNAC	8 cut(s)	74, 488, 860, 1006, 1250, 1288, 1490, 1982
MflI	RGATCY	4 cut(s)	81, 700, 1134, 2163
MhlI	GDGCHC	5 cut(s)	19, 31, 312, 342, 1656
MlsI	TGGCCA	3 cut(s)	906, 1156, 1479
MluNI	TGGCCA	3 cut(s)	906, 1156, 1479
MlyI	GAGTC	4 cut(s)	2, 1498, 1853, 2353
MmeI	TCCRAC	2 cut(s)	171, 781
Mox20I	TGGCCA	3 cut(s)	906, 1156, 1479
MroXI	GAANNNNTTC	1 cut(s)	162
MscI	TGGCCA	3 cut(s)	906, 1156, 1479
MseI	TTAA	3 cut(s)	384, 957, 1809
MslI	CAYNNNNRTG	2 cut(s)	1689, 2360
Msp20I	TGGCCA	3 cut(s)	906, 1156, 1479
MspA1I	CMGCKG	2 cut(s)	142, 1397
MspI	CCGG	3 cut(s)	1458, 1512, 1640
Mva1269I	GAATGC	2 cut(s)	613, 996
MvaI	CCWGG	9 cut(s)	227, 274, 279, 908, 1211, 1448, 1575, 1850, 2225
MvnI	CGCG	2 cut(s)	741, 1950
MwoI	GCNNNNNNNGC	6 cut(s)	139, 535, 567, 1801, 1915, 2088
NciI	CCSGG	2 cut(s)	1512, 1640
NcoI	CCATGG	3 cut(s)	248, 825, 2143
NlaIV	GGNNCC	5 cut(s)	783, 890, 1016, 2126, 2282
NmeAIII	GCCGAG	2 cut(s)	349, 661
NmuCI	GTSAC	4 cut(s)	488, 1006, 1490, 1982
NspI	RCATGY	2 cut(s)	529, 2321
OliI	CACNNNNGTG	1 cut(s)	1689
PaeI	GCATGC	1 cut(s)	529
PaeR7I	CTCGAG	1 cut(s)	539
PasI	CCCWGGG	1 cut(s)	2224
PceI	AGGCCT	2 cut(s)	277, 2232
PciSI	GCTCTTC	2 cut(s)	512, 1644
PcsI	WCGNNNNNNNCGW	1 cut(s)	738
PctI	GAATGC	2 cut(s)	613, 996
PdmI	GAANNNNTTC	1 cut(s)	162
PfeI	GAWTC	2 cut(s)	1337, 2396
PflMI	CCANNNNNTGG	2 cut(s)	1315, 2144
PfoI	TCCNGGA	1 cut(s)	1446
PkrI	GCNGC	3 cut(s)	132, 2007, 2158
PleI	GAGTC	4 cut(s)	2, 1497, 1852, 2352
PmaCI	CACGTG	1 cut(s)	14
PmlI	CACGTG	1 cut(s)	14
PpsI	GAGTC	4 cut(s)	2, 1497, 1852, 2352
Ppu21I	YACGTR	1 cut(s)	14
Psp124BI	GAGCTC	1 cut(s)	1656
Psp6I	CCWGG	9 cut(s)	225, 272, 277, 906, 1209, 1446, 1573, 1848, 2223
PspCI	CACGTG	1 cut(s)	14
PspFI	CCCAGC	2 cut(s)	1826, 2092
PspGI	CCWGG	9 cut(s)	225, 272, 277, 906, 1209, 1446, 1573, 1848, 2223
PspN4I	GGNNCC	5 cut(s)	783, 890, 1016, 2126, 2282
PspPI	GGNCC	2 cut(s)	1741, 2090
PspXI	VCTCGAGB	1 cut(s)	539
PstNI	CAGNNNCTG	1 cut(s)	1580
PsuI	RGATCY	4 cut(s)	81, 700, 1134, 2163
PvuII	CAGCTG	2 cut(s)	142, 1397
RseI	CAYNNNNRTG	2 cut(s)	1689, 2360
SacI	GAGCTC	1 cut(s)	1656
SapI	GCTCTTC	2 cut(s)	512, 1644
SaqAI	TTAA	3 cut(s)	384, 957, 1809
SatI	GCNGC	3 cut(s)	131, 2006, 2157
Sau96I	GGNCC	2 cut(s)	1741, 2090
ScaI	AGTACT	1 cut(s)	347
SchI	GAGTC	4 cut(s)	2, 1498, 1853, 2353
SduI	GDGCHC	5 cut(s)	19, 31, 312, 342, 1656
SfaNI	GCATC	3 cut(s)	570, 942, 1015
SfcI	CTRYAG	2 cut(s)	1240, 1660
Sfr274I	CTCGAG	1 cut(s)	539
SinI	GGWCC	1 cut(s)	1741
SlaI	CTCGAG	1 cut(s)	539
SmiMI	CAYNNNNRTG	2 cut(s)	1689, 2360
SmlI	CTYRAG	6 cut(s)	539, 776, 1055, 1138, 2339, 2375
SmoI	CTYRAG	6 cut(s)	539, 776, 1055, 1138, 2339, 2375
SphI	GCATGC	1 cut(s)	529
SseBI	AGGCCT	2 cut(s)	277, 2232
SsiI	CCGC	4 cut(s)	5, 1126, 1497, 1948
SspMI	CTAG	3 cut(s)	86, 1163, 1361
SstI	GAGCTC	1 cut(s)	1656
StuI	AGGCCT	2 cut(s)	277, 2232
StyI	CCWWGG	6 cut(s)	111, 248, 811, 825, 1989, 2143
TaiI	ACGT	2 cut(s)	16, 964
TaqI	TCGA	5 cut(s)	474, 540, 1834, 2244, 2358
TatI	WGTACW	4 cut(s)	291, 345, 2253, 2379
TfiI	GAWTC	2 cut(s)	1337, 2396
Tru1I	TTAA	3 cut(s)	384, 957, 1809
Tru9I	TTAA	3 cut(s)	384, 957, 1809
TscAI	CASTG	6 cut(s)	264, 563, 904, 1249, 1765, 2213
TseFI	GTSAC	4 cut(s)	488, 1006, 1490, 1982
TseI	GCWGC	3 cut(s)	130, 2005, 2156
Tsp45I	GTSAC	4 cut(s)	488, 1006, 1490, 1982
TspDTI	ATGAA	5 cut(s)	1417, 1427, 1520, 1958, 2034
TspRI	CASTG	6 cut(s)	264, 563, 904, 1249, 1765, 2213
Van91I	CCANNNNNTGG	2 cut(s)	1315, 2144
VneI	GTGCAC	1 cut(s)	308
VpaK11BI	GGWCC	1 cut(s)	1741
XapI	RAATTY	5 cut(s)	184, 200, 594, 806, 819
XceI	RCATGY	2 cut(s)	529, 2321
XhoI	CTCGAG	1 cut(s)	539
XmnI	GAANNNNTTC	1 cut(s)	162
XspI	CTAG	3 cut(s)	86, 1163, 1361
ZrmI	AGTACT	1 cut(s)	347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.