Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Reverse (-)

8164061 .. 8167409

Gene Structure 3349 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016605

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGCTGACTCGCGTGTTCTGAGCCGTGTCCACAGCCTCAGGGAGAGGCTGGATGACACACTTTCTGCTCACCGCAACGAGATCTTGGGCTTTCTTTCAAGGATTGAAGCCAAGGGAAAAGGGTTTTTGCAGCCCCATCAGCTGATTGATGAGTTTGAAGCAATTCCTGAAGCCAGCAGACAAAAATTGTCGGAATCTGCATTTGGGGAAGTTCTGAAGAACACCCAGGAAGCTATTGTTCTGCCTCCATGGGTGGCACTCGCTGTTCGTCCCAGGCCTGGAGTTTGGGAGTACATTCGGGTCAATGTGCACTCACTTGTTGCCGAGGAGTTGCTTGTGCCCGAGTACTTGCACTTCAAAGAGGAGCTTGTTGATGGAAGTCTCAATGGCAACTTTGTGCTTGAATTGGACTTTGAACCGTTCAATGCCTCTTTCCCCAAACCAACTCTTTCCAAATCTATTGGAAATGGTGTCGAGTTCCTCAACCGTCACCTCTCTGCAAAACTCTTCCATGACAAGGAGAGCATGCACCCACTGCTTGAGTTTCTCCGAGTTCACTGCTACAAGGGCAAGAATATGATGCTGAATGACAGAATTCAGAATGTGAATGCACTTCAACATGTTCTGAGGAAGGCAGAGGAGTTACTGGCTACACTCGCACCAGAAACCCCATACAAGAGTTTTGAGATCAAGTTGCAGGATCTTGGTTTGGAGAGAGGTTGGGGAGACAACGCTGGGCGCGTGCTCGAAATGATCCAACTCCTCTTGGAGCTCCTTGAGGCACCTGATCCTTGCAATCTAGAAAAGTTCCTTGGCAGAATTCCCATGGTGTTCAATGTTGTGATCATGACTCCTCATGGTTACTTTGCCCAAGACAATGTGTTGGGGTACCCTGACACTGGTGGTCAGGTTGTTTATATCTTGGATCAAGTTCGTGCGTTGGAGAATGAGATGCTTAAACGTATCAAGGAACAAGGGCTAGATATCATCCCTCGCATTCTTGTTGTCACAAGGCTCCTTCCTGATGCCGTAGGAACTACTTGTGGTCAGCGTCTTGAGAAGGTGTACAATACCAAACATTCTGATATTCTTCGTGTTCCCTTTAGAACCGAGAAGGGAATTGTCCGCAAATGGATCTCAAGATTCGAAGTGTGGCCATACCTAGAGACTTACACCGAGGATGTTGCAACAGAACTTGCTAAAGAGTTCCAGGGCAAGCCTGATCTCATTATTGGAAACTACAGTGACGGTAACATTGTGGCCTCATTGTTGGCACATAAATTGAGTGTTACTCAGTGCACCATTGCCCATGCTTTGGAGAAGACAAAGTACCCTGATTCAGACCTTTACTGGAAGAAACTAGACGACAAGTACCACTTCTCTTGCCAATTCACAGCTGATCTTATCGCCATGAACCACACTGACTTCATTATCACCAGTACCTTCCAGGAGATTGCTGGAAGCAAAGATACTGTTGGTCAGTATGAGAGTCACACCGCCTTCACCATGCCTGGTCTCTACCGTGTTGTTCATGGGATTGATGTCTTTGATCCCAAGTTCAACATTGTTTCACCTGGAGCCGACATGGACATTTACTTCCCTTACACCGAGAAGGAAAAGAGGTTGACACATTTCCACCCTGAAATCGAAGAGCTCATCTACAGCCAAGAGGAGAACAAAGAACACTTGTGTGTGTTGAAGGACAGGAACAAGCCAGTCCTCTTCACAATGGCAAGGCTGGACCGTGTAAAGAACATCACTGGGCTGGTTGAGTGGTATGGTAAGAACAAGAAGCTTAGGGAGCTGGTTAACTTGGTTGTTGTTGCTGGGGATCGCCGAAAGGAGTCCCAGGACAATGAGGAGAAAGCTGAGCTGAAGAAGATGTATGAGCTGATTGACACCTACAAGCTGAATGGGCAGTTCAGATGGATTTCCTCTCAGATGAATCGGGTGAGGAACGGTGAACTCTATCGTGTCATTGCTGACAGCAAGGGAGCTTTTGTGCAGCCTGCTGTGTATGAAGCCTTTGGCTTGACTGTTGTTGAGGCAATGACTTGTGGTTTGCCCACATTTGCTACTTGCAAAGGTGGCCCAGCTGAGATTATTGTGAACGGAAAATCTGGGTACCATATTGATCCATACCACGGTGAGCAAGCTGCTGAGATCATTGTTGACTTCTTTGAGAAGTGTAAGAAAGACCCTTCTCACTGGGACAAGATTTCCCAGGGAGGCTTGAAGCGTATCTATGAGAAGTACACATGGCAAATCTACTCTGAAAGGCTCTTGACCTTGACTGGAGTTTATGGCTTCTGGAAGCATGTCTCCAACCTTGATCGCCTTGAGAGCCGTCGCTACATTGAGATGTTCTATGCTCTCAAGTACAAAAAGCTGGCTGAGTCTGTTCCTCTTGCTAAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

807

Amino Acids

92.55

Weight (kDa)

6.25

Isoelectric Point (pI)

30.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	9 - 121	1.6e-45	Sucrose synthase N-terminal
SUS_EPBD	PF24862	156 - 243	8.7e-34	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	266 - 554	3.5e-170	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	567 - 737	5.4e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	572 - 726	1.9e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	888, 2124
AccB1I	GGYRCC	3 cut(s)	781, 888, 2124
AccB7I	CCANNNNNTGG	1 cut(s)	1315
AccII	CGCG	2 cut(s)	13, 741
AciI	CCGC	3 cut(s)	73, 1126, 1497
AclWI	GGATC	8 cut(s)	708, 748, 782, 933, 1142, 1544, 1839, 2129
AcoI	YGGCCR	1 cut(s)	1154
AcsI	RAATTY	2 cut(s)	594, 819
AcuI	CTGAAG	3 cut(s)	189, 236, 1895
AfiI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
AflIII	ACRYGT	1 cut(s)	619
AjnI	CCWGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
AjuI	GAANNNNNNNTTGG	4 cut(s)	186, 218, 795, 827
AleI	CACNNNNGTG	1 cut(s)	1689
Alw21I	GWGCWC	5 cut(s)	312, 747, 774, 1301, 1656
Alw26I	GTCTC	5 cut(s)	386, 720, 1160, 1520, 2326
Alw44I	GTGCAC	2 cut(s)	308, 1297
AlwI	GGATC	8 cut(s)	708, 748, 782, 933, 1142, 1544, 1839, 2129
Ama87I	CYCGRG	1 cut(s)	341
AoxI	GGCC	4 cut(s)	275, 1154, 1260, 2089
ApaLI	GTGCAC	2 cut(s)	308, 1297
ApeKI	GCWGC	3 cut(s)	130, 2005, 2156
ApoI	RAATTY	2 cut(s)	594, 819
ArsI	GACNNNNNNTTYG	2 cut(s)	2044, 2076
Asp700I	GAANNNNTTC	1 cut(s)	162
Asp718I	GGTACC	2 cut(s)	888, 2124
AspLEI	GCGC	1 cut(s)	741
AspS9I	GGNCC	2 cut(s)	1741, 2090
AsuHPI	GGTGA	8 cut(s)	62, 482, 1426, 1495, 1563, 1963, 1973, 2159
AsuII	TTCGAA	1 cut(s)	1146
AvaI	CYCGRG	1 cut(s)	341
AvaII	GGWCC	1 cut(s)	1741
AxyI	CCTNAGG	1 cut(s)	38
BaeGI	GKGCMC	3 cut(s)	312, 342, 1301
BalI	TGGCCA	1 cut(s)	1156
BanI	GGYRCC	3 cut(s)	781, 888, 2124
BanII	GRGCYC	2 cut(s)	774, 1656
BarI	GAAGNNNNNNTAC	6 cut(s)	338, 370, 1313, 1345, 1453, 1485
BbsI	GAAGAC	1 cut(s)	1328
Bbv12I	GWGCWC	5 cut(s)	312, 747, 774, 1301, 1656
BbvI	GCAGC	3 cut(s)	142, 2017, 2143
BccI	CCATC	3 cut(s)	144, 368, 1920
BceAI	ACGGC	3 cut(s)	9, 1013, 2331
BcgI	CGANNNNNNTGC	4 cut(s)	540, 574, 1166, 1200
BciT130I	CCWGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
BclI	TGATCA	1 cut(s)	843
BcoDI	GTCTC	5 cut(s)	386, 720, 1160, 1520, 2326
BfaI	CTAG	4 cut(s)	800, 980, 1163, 1361
BfmI	CTRYAG	2 cut(s)	1240, 1660
BglII	AGATCT	1 cut(s)	81
BisI	GCNGC	3 cut(s)	131, 2006, 2157
BlpI	GCTNAGC	1 cut(s)	1869
BlsI	GCNGC	3 cut(s)	132, 2007, 2158
BmcAI	AGTACT	1 cut(s)	347
Bme1390I	CCNGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
Bme18I	GGWCC	1 cut(s)	1741
BmeT110I	CYCGRG	1 cut(s)	341
BmgT120I	GGNCC	2 cut(s)	1741, 2090
BmiI	GGNNCC	5 cut(s)	783, 890, 1016, 1579, 2126
BmrFI	CCNGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
BmrI	ACTGGG	2 cut(s)	1770, 2218
BmsI	GCATC	3 cut(s)	570, 942, 1015
BmuI	ACTGGG	2 cut(s)	1770, 2218
BpiI	GAAGAC	1 cut(s)	1328
BplI	GAGNNNNNCTC	2 cut(s)	1276, 1308
BpmI	CTGGAG	3 cut(s)	300, 1596, 2316
Bpu10I	CCTNAGC	2 cut(s)	1796, 2412
Bpu1102I	GCTNAGC	1 cut(s)	1869
Bpu14I	TTCGAA	1 cut(s)	1146
BpuEI	CTTGAG	6 cut(s)	560, 797, 1076, 1123, 2360, 2360
BsaI	GGTCTC	1 cut(s)	1520
BsaXI	ACNNNNNCTCC	2 cut(s)	1986, 2016
Bsc4I	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
Bse1I	ACTGG	8 cut(s)	651, 904, 1355, 1437, 1715, 1765, 2213, 2299
Bse21I	CCTNAGG	1 cut(s)	38
Bse3DI	GCAATG	3 cut(s)	1302, 1977, 2055
BseBI	CCWGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
BseGI	GGATG	3 cut(s)	58, 987, 1186
BseLI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
BseMI	GCAATG	3 cut(s)	1302, 1977, 2055
BseMII	CTCAG	9 cut(s)	11, 52, 617, 1307, 1860, 1952, 2088, 2151, 2385
BseNI	ACTGG	8 cut(s)	651, 904, 1355, 1437, 1715, 1765, 2213, 2299
BseRI	GAGGAG	7 cut(s)	341, 377, 653, 752, 843, 1685, 1874
BseSI	GKGCMC	3 cut(s)	312, 342, 1301
BseXI	GCAGC	3 cut(s)	142, 2017, 2143
BseYI	CCCAGC	3 cut(s)	734, 1826, 2092
BsgI	GTGCAG	1 cut(s)	2024
Bsh1236I	CGCG	2 cut(s)	13, 741
BshFI	GGCC	4 cut(s)	277, 1156, 1262, 2091
BshNI	GGYRCC	3 cut(s)	781, 888, 2124
BsiHKAI	GWGCWC	5 cut(s)	312, 747, 774, 1301, 1656
BsiHKCI	CYCGRG	1 cut(s)	341
BslFI	GGGAC	3 cut(s)	255, 1831, 2225
BslI	CCNNNNNNNGG	5 cut(s)	278, 517, 899, 1315, 2144
BsmAI	GTCTC	5 cut(s)	386, 720, 1160, 1520, 2326
BsmFI	GGGAC	3 cut(s)	255, 1831, 2225
BsmI	GAATGC	2 cut(s)	613, 996
BsnI	GGCC	4 cut(s)	277, 1156, 1262, 2091
Bso31I	GGTCTC	1 cut(s)	1520
BsoBI	CYCGRG	1 cut(s)	341
Bsp119I	TTCGAA	1 cut(s)	1146
Bsp1286I	GDGCHC	6 cut(s)	312, 342, 747, 774, 1301, 1656
Bsp1407I	TGTACA	1 cut(s)	1065
Bsp1720I	GCTNAGC	1 cut(s)	1869
Bsp19I	CCATGG	2 cut(s)	248, 825
BspACI	CCGC	3 cut(s)	73, 1126, 1497
BspANI	GGCC	4 cut(s)	277, 1156, 1262, 2091
BspCNI	CTCAG	9 cut(s)	12, 51, 618, 1306, 1861, 1951, 2089, 2152, 2386
BspFNI	CGCG	2 cut(s)	13, 741
BspHI	TCATGA	1 cut(s)	846
BspLI	GGNNCC	5 cut(s)	783, 890, 1016, 1579, 2126
BspPI	GGATC	8 cut(s)	708, 748, 782, 933, 1142, 1544, 1839, 2129
BspQI	GCTCTTC	1 cut(s)	1644
BspT104I	TTCGAA	1 cut(s)	1146
BspT107I	GGYRCC	3 cut(s)	781, 888, 2124
BspTNI	GGTCTC	1 cut(s)	1520
BsrDI	GCAATG	3 cut(s)	1302, 1977, 2055
BsrGI	TGTACA	1 cut(s)	1065
BsrI	ACTGG	8 cut(s)	651, 904, 1355, 1437, 1715, 1765, 2213, 2299
BssT1I	CCWWGG	4 cut(s)	111, 248, 811, 825
Bst2UI	CCWGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
Bst6I	CTCTTC	3 cut(s)	512, 1644, 1727
BstAPI	GCANNNNNTGC	1 cut(s)	535
BstAUI	TGTACA	1 cut(s)	1065
BstBI	TTCGAA	1 cut(s)	1146
BstC8I	GCNNGC	7 cut(s)	175, 527, 743, 1217, 2010, 2154, 2391
BstDSI	CCRYGG	3 cut(s)	248, 825, 2143
BstF5I	GGATG	3 cut(s)	58, 987, 1186
BstFNI	CGCG	2 cut(s)	13, 741
BstHHI	GCGC	1 cut(s)	741
BstMAI	GTCTC	5 cut(s)	386, 720, 1160, 1520, 2326
BstMWI	GCNNNNNNNGC	8 cut(s)	139, 535, 567, 656, 1801, 1915, 2088, 2343
BstNI	CCWGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
BstNSI	RCATGY	3 cut(s)	529, 623, 2321
BstSCI	CCNGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
BstSFI	CTRYAG	2 cut(s)	1240, 1660
BstSLI	GKGCMC	3 cut(s)	312, 342, 1301
BstUI	CGCG	2 cut(s)	13, 741
BstV1I	GCAGC	3 cut(s)	142, 2017, 2143
BstV2I	GAAGAC	1 cut(s)	1328
BstX2I	RGATCY	3 cut(s)	81, 700, 1134
BstYI	RGATCY	3 cut(s)	81, 700, 1134
Bsu36I	CCTNAGG	1 cut(s)	38
BsuRI	GGCC	4 cut(s)	277, 1156, 1262, 2091
BtgI	CCRYGG	3 cut(s)	248, 825, 2143
BtsCI	GGATG	3 cut(s)	58, 987, 1186
BtsI	GCAGTG	2 cut(s)	533, 556
BtsIMutI	CAGTG	8 cut(s)	533, 556, 897, 1249, 1301, 1419, 1758, 2206
Cac8I	GCNNGC	7 cut(s)	175, 527, 743, 1217, 2010, 2154, 2391
CciI	TCATGA	1 cut(s)	846
CfoI	GCGC	1 cut(s)	741
Cfr13I	GGNCC	2 cut(s)	1741, 2090
CseI	GACGC	1 cut(s)	1040
CspCI	CAANNNNNGTGG	2 cut(s)	1364, 1399
EaeI	YGGCCR	1 cut(s)	1154
Eam1104I	CTCTTC	3 cut(s)	512, 1644, 1727
EarI	CTCTTC	3 cut(s)	512, 1644, 1727
Ecl136II	GAGCTC	2 cut(s)	772, 1654
Eco130I	CCWWGG	4 cut(s)	111, 248, 811, 825
Eco147I	AGGCCT	1 cut(s)	277
Eco24I	GRGCYC	2 cut(s)	774, 1656
Eco31I	GGTCTC	1 cut(s)	1520
Eco32I	GATATC	1 cut(s)	985
Eco47I	GGWCC	1 cut(s)	1741
Eco53kI	GAGCTC	2 cut(s)	772, 1654
Eco57I	CTGAAG	3 cut(s)	189, 236, 1895
Eco81I	CCTNAGG	1 cut(s)	38
Eco88I	CYCGRG	1 cut(s)	341
EcoICRI	GAGCTC	2 cut(s)	772, 1654
EcoRI	GAATTC	2 cut(s)	594, 819
EcoRII	CCWGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
EcoRV	GATATC	1 cut(s)	985
EcoT14I	CCWWGG	4 cut(s)	111, 248, 811, 825
EcoT38I	GRGCYC	2 cut(s)	774, 1656
ErhI	CCWWGG	4 cut(s)	111, 248, 811, 825
FalI	AAGNNNNNCTT	4 cut(s)	351, 383, 1361, 1393
FaqI	GGGAC	3 cut(s)	255, 1831, 2225
FbaI	TGATCA	1 cut(s)	843
Fnu4HI	GCNGC	3 cut(s)	131, 2006, 2157
FokI	GGATG	3 cut(s)	65, 974, 1193
FriOI	GRGCYC	2 cut(s)	774, 1656
Fsp4HI	GCNGC	3 cut(s)	131, 2006, 2157
FspBI	CTAG	4 cut(s)	800, 980, 1163, 1361
GlaI	GCGC	1 cut(s)	740
GluI	GCNGC	3 cut(s)	131, 2006, 2157
GsaI	CCCAGC	3 cut(s)	738, 1830, 2096
GsuI	CTGGAG	3 cut(s)	300, 1596, 2316
HaeIII	GGCC	4 cut(s)	277, 1156, 1262, 2091
HgaI	GACGC	1 cut(s)	1040
HhaI	GCGC	1 cut(s)	741
Hin6I	GCGC	1 cut(s)	739
HinP1I	GCGC	1 cut(s)	739
HincII	GTYRAC	3 cut(s)	1626, 1810, 2173
HindII	GTYRAC	3 cut(s)	1626, 1810, 2173
HindIII	AAGCTT	1 cut(s)	1793
HinfI	GANTC	9 cut(s)	8, 194, 850, 1143, 1337, 1489, 1844, 1945, 2396
HpaI	GTTAAC	1 cut(s)	1810
HphI	GGTGA	8 cut(s)	62, 482, 1426, 1495, 1563, 1963, 1973, 2159
Hpy188III	TCNNGA	8 cut(s)	167, 800, 847, 1022, 1055, 1140, 2284, 2311
Hpy99I	CGWCG	1 cut(s)	2352
HpyAV	CCTTC	9 cut(s)	625, 1028, 1054, 1108, 1453, 1510, 1606, 1693, 2211
HpyCH4IV	ACGT	1 cut(s)	961
HpyF10VI	GCNNNNNNNGC	8 cut(s)	139, 535, 567, 656, 1801, 1915, 2088, 2343
HpySE526I	ACGT	1 cut(s)	961
HspAI	GCGC	1 cut(s)	739
KpnI	GGTACC	2 cut(s)	892, 2128
Ksp22I	TGATCA	1 cut(s)	843
KspAI	GTTAAC	1 cut(s)	1810
LguI	GCTCTTC	1 cut(s)	1644
LmnI	GCTCC	7 cut(s)	364, 769, 777, 1020, 1577, 1801, 1994
Lsp1109I	GCAGC	3 cut(s)	142, 2017, 2143
LweI	GCATC	3 cut(s)	570, 942, 1015
MaeI	CTAG	4 cut(s)	800, 980, 1163, 1361
MaeII	ACGT	1 cut(s)	961
MaeIII	GTNAC	8 cut(s)	488, 642, 860, 1006, 1244, 1250, 1288, 1490
MboII	GAAGA	9 cut(s)	229, 499, 1082, 1333, 1366, 1661, 1714, 1888, 1891
MflI	RGATCY	3 cut(s)	81, 700, 1134
MhlI	GDGCHC	6 cut(s)	312, 342, 747, 774, 1301, 1656
MlsI	TGGCCA	1 cut(s)	1156
MluCI	AATT	8 cut(s)	162, 185, 404, 594, 819, 1119, 1280, 1388
MluNI	TGGCCA	1 cut(s)	1156
MlyI	GAGTC	5 cut(s)	2, 844, 1498, 1853, 2405
MmeI	TCCRAC	4 cut(s)	171, 781, 921, 2349
Mox20I	TGGCCA	1 cut(s)	1156
MroXI	GAANNNNTTC	1 cut(s)	162
MscI	TGGCCA	1 cut(s)	1156
MseI	TTAA	2 cut(s)	957, 1809
MslI	CAYNNNNRTG	2 cut(s)	1689, 2360
Msp20I	TGGCCA	1 cut(s)	1156
MspA1I	CMGCKG	3 cut(s)	142, 1397, 2096
MspR9I	CCNGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
Mva1269I	GAATGC	2 cut(s)	613, 996
MvaI	CCWGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
MvnI	CGCG	2 cut(s)	13, 741
MwoI	GCNNNNNNNGC	8 cut(s)	139, 535, 567, 656, 1801, 1915, 2088, 2343
NcoI	CCATGG	2 cut(s)	248, 825
NlaIV	GGNNCC	5 cut(s)	783, 890, 1016, 1579, 2126
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	4 cut(s)	488, 1006, 1244, 1490
NspI	RCATGY	3 cut(s)	529, 623, 2321
NspV	TTCGAA	1 cut(s)	1146
OliI	CACNNNNGTG	1 cut(s)	1689
PaeI	GCATGC	1 cut(s)	529
PagI	TCATGA	1 cut(s)	846
PasI	CCCWGGG	1 cut(s)	2224
PceI	AGGCCT	1 cut(s)	277
PciI	ACATGT	1 cut(s)	619
PciSI	GCTCTTC	1 cut(s)	1644
PcsI	WCGNNNNNNNCGW	1 cut(s)	738
PctI	GAATGC	2 cut(s)	613, 996
PdmI	GAANNNNTTC	1 cut(s)	162
PfeI	GAWTC	4 cut(s)	194, 1143, 1337, 1945
PflMI	CCANNNNNTGG	1 cut(s)	1315
PfoI	TCCNGGA	1 cut(s)	1446
PkrI	GCNGC	3 cut(s)	132, 2007, 2158
PleI	GAGTC	5 cut(s)	2, 844, 1497, 1852, 2404
PpsI	GAGTC	5 cut(s)	2, 844, 1497, 1852, 2404
PscI	ACATGT	1 cut(s)	619
Psp124BI	GAGCTC	2 cut(s)	774, 1656
Psp6I	CCWGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
PspFI	CCCAGC	3 cut(s)	734, 1826, 2092
PspGI	CCWGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
PspN4I	GGNNCC	5 cut(s)	783, 890, 1016, 1579, 2126
PspPI	GGNCC	2 cut(s)	1741, 2090
PsuI	RGATCY	3 cut(s)	81, 700, 1134
PvuII	CAGCTG	3 cut(s)	142, 1397, 2096
RseI	CAYNNNNRTG	2 cut(s)	1689, 2360
SacI	GAGCTC	2 cut(s)	774, 1656
SapI	GCTCTTC	1 cut(s)	1644
SaqAI	TTAA	2 cut(s)	957, 1809
SatI	GCNGC	3 cut(s)	131, 2006, 2157
Sau96I	GGNCC	2 cut(s)	1741, 2090
ScaI	AGTACT	1 cut(s)	347
SchI	GAGTC	5 cut(s)	2, 844, 1498, 1853, 2405
ScrFI	CCNGG	9 cut(s)	227, 274, 279, 1211, 1448, 1512, 1575, 1850, 2225
SduI	GDGCHC	6 cut(s)	312, 342, 747, 774, 1301, 1656
SfaNI	GCATC	3 cut(s)	570, 942, 1015
SfcI	CTRYAG	2 cut(s)	1240, 1660
SfuI	TTCGAA	1 cut(s)	1146
SinI	GGWCC	1 cut(s)	1741
SmiMI	CAYNNNNRTG	2 cut(s)	1689, 2360
SmlI	CTYRAG	6 cut(s)	539, 776, 1055, 1138, 2339, 2375
SmoI	CTYRAG	6 cut(s)	539, 776, 1055, 1138, 2339, 2375
SphI	GCATGC	1 cut(s)	529
Sse9I	AATT	8 cut(s)	162, 185, 404, 594, 819, 1119, 1280, 1388
SseBI	AGGCCT	1 cut(s)	277
SsiI	CCGC	3 cut(s)	73, 1126, 1497
SspMI	CTAG	4 cut(s)	800, 980, 1163, 1361
SstI	GAGCTC	2 cut(s)	774, 1656
StuI	AGGCCT	1 cut(s)	277
StyD4I	CCNGG	9 cut(s)	225, 272, 277, 1209, 1446, 1510, 1573, 1848, 2223
StyI	CCWWGG	4 cut(s)	111, 248, 811, 825
TaiI	ACGT	1 cut(s)	964
TaqI	TCGA	4 cut(s)	474, 747, 1146, 1647
TasI	AATT	8 cut(s)	162, 185, 404, 594, 819, 1119, 1280, 1388
TatI	WGTACW	5 cut(s)	291, 345, 1065, 2253, 2379
TfiI	GAWTC	4 cut(s)	194, 1143, 1337, 1945
Tru1I	TTAA	2 cut(s)	957, 1809
Tru9I	TTAA	2 cut(s)	957, 1809
TscAI	CASTG	8 cut(s)	540, 563, 904, 1249, 1301, 1426, 1765, 2213
TseFI	GTSAC	4 cut(s)	488, 1006, 1244, 1490
TseI	GCWGC	3 cut(s)	130, 2005, 2156
Tsp45I	GTSAC	4 cut(s)	488, 1006, 1244, 1490
TspDTI	ATGAA	5 cut(s)	1417, 1427, 1520, 1958, 2034
TspGWI	ACGGA	1 cut(s)	2127
TspRI	CASTG	8 cut(s)	540, 563, 904, 1249, 1301, 1426, 1765, 2213
Van91I	CCANNNNNTGG	1 cut(s)	1315
VneI	GTGCAC	2 cut(s)	308, 1297
VpaK11BI	GGWCC	1 cut(s)	1741
XapI	RAATTY	2 cut(s)	594, 819
XbaI	TCTAGA	1 cut(s)	799
XceI	RCATGY	3 cut(s)	529, 623, 2321
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1454
XmnI	GAANNNNTTC	1 cut(s)	162
XspI	CTAG	4 cut(s)	800, 980, 1163, 1361
ZrmI	AGTACT	1 cut(s)	347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.