Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Forward (+)

86157341 .. 86160205

Gene Structure 2865 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00148460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGGCCGATCCTTCTTCATGTGCTCATTGCAGCATCTATGAGAGGTTGGACTGGACTGAGTCTGTTCACCGTGATGAGATCTTGGCTGTGCTTTCGAGGGTTGAAAGCAAGGGCAAAGGGTTTGTCCAACCCCAGCAACTTGTTGCTGAGTTTGAAGCAATCCCTGAAGCATTTGGAGAAGTTTCAAAGAACATTCAGGAAGCTGTTGTTCTATCTCCATACCTAGCCCTCGCTCTTCGTCCAAGGCCCGGTGTTTGGGAACACATCCGAATCAACATGAAGACACTCGAGGTTGAGGAGTTGCCTGTGGCTGAGTACTTGAAGATGAAGGAGGAACTCGTCGATGGAAGTGCCAATGGAGGCTTTGTGCTGGAGTTGGACTTTGAGCCATTCAATGCTTCAGTTCCCAAACCAACTCTTTCCAAGTCCATTGGAAACGGCGTTGAGTACCTCAACCGCCACCTCTCTGCAAAGCTCTTCACTGAGCCGGAGAGCATGCATCCAGTGTTGCTCGAGTTTCTCCGCCTTCACACTCATGACGGCAAGAACCTGATGCTGAATGACAACATTGAGGATGTGGAAGAACTCAAAGATGTTCTCATGGAGGTGGAGAAGTATCTCTCTTCACTAGCCCCCGGAACTCCATATGAAGCTTTCGAGCATAAGTTGCAAGATCTTGGCTTGGAGAGAGGTTGGGGAAACAATGCTGAGCGTGCGCTTGAAACGATCCAGCTTCTCTTGGACCTTCTTGTGGCACCAGATGCTTGCAATCTCGAGAAATTCATCAGCAAGATCCCCTTGGTCTTTAATGTTGTGATCATGTCTCCCCATGGTTATTTTGCACAAGACAATGTCTTGGGATATCCTGACACTGGTGGCCAGGTTGTTTATATCTTGGATCAAGTGCGTGCTTTGGAGAGTGAGATGCTTAAGCGTATCAAGGAACAAGGGCTTGATATCACCCCTCGCATTCTTATTGTTACAAGGCTCCTTCCTGATGCTGTCGGGACTACTTGCGGTCAGCGTCTTGAGAGGGTGTATGGAACTGAATATTCGGATATTATCCGTGTTCCTTTCAGAACAGAGAAGGGAATTGTCCGTAGATGGATCTCAAGATTTGAAGTCTGGCCATATCTAGAGACTTACACTGAGGATGTTGCAACTGAACTTCTCAAGGAGTTCCAGGGCAAGCCTGATCTTATCGTCGGAAACTACAGTGATGGAAACATTGTGGCTTCATTGTTGGCACACAAATTTGATGTTACTCAGTGCACCATTGCTCATGCCTTGGAGAAGACAAAGTACCCTGATTCTGACTTGTACTGGAAGAAACTTGAGGACAAGTACCACTTCTCTTGCCAGTTCACAGCTGATCTCATTGCCATGAACCAGACCGATTTCATTATCACAAGTACCTTCCAAGAGATTGCTGGAAGCAAAGACACTGTTGGTCAATATGAGACTCACATTGCCTTCACAATGCCAGGCCTCTACCGCGTTGTCAATGGTATAGATTGCTTCGATCCCAAGTTCAACATTGTTTCCCCAGGAGCTGACATGGACATTTACTTCCCGTACACTGAGAAGGAAAGACGGTTGACATCATTCCACCCCGAAATCGAAGAGCTCATCTACAGCCAAGTTGAGAATAAGGAGCACTTGTGTGTCTTGAATGACACGAACAAGCCTATCATCTTCACAATGGCAAGGCTTGACCGTGTGAAGAACATCACCGGGCTTGTGGAATGGTATGGCAAGAATGCCAAGCTTAGGGAGTTGGTTAACCTTGTGGTTGTGGCTGGGGATCGGCGAAAGGAGTCCAAGGACAACGAGGAGAAGGCTGAGATGAAGAAGATGTACGAGCTCATTGACACCTACAAGTTGAATGGACAGTTCAGATGGATTTCTTCTCAGATGAACCGCGTGAGGAACGGTGAGCTCTACCGCTATATTGCTGACACTAAAGGAGCTTTTGTGCAACCTGCTGTCTATGAAGCCTTTGGCTTGACTGTTGTGGAGGCCATGACTTGTGGATTGCCTACCTTTGCTACTTGCAAAGGCGGCCCGGCTGAAATTATTGTGAATGGAAAATCCGGCTACCACATTGATCCATATCATGGTGAAAAAGCTGCTGAGATCCTTGTTGACTTCTTTCAGAAGTGCAAGGTTGATCCTTCTCACTGGGACAAGATCTCCCAAGGAGGCTTGAAGCGTATCGAAGAGAAGTACACTTGGCAGATTTACTCTGAGAGGCTGCTGACCCTGACCGGAGTTTATGGCTTCTGGAAGCATGTTTCTAACCTTGACGAGAAGCGTCGCTACATAGAAATGTTGTATGCCCTCCGGTATAAGAAATCGGTTGAGTCTGTTCCTCTTGCTGAAGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

796

Amino Acids

90.96

Weight (kDa)

5.26

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	11 - 111	1.6e-29	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 234	1e-30	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	257 - 545	9.6e-166	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	558 - 714	7.7e-26	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	563 - 715	5.5e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1990
AccB1I	GGYRCC	1 cut(s)	754
AccB7I	CCANNNNNTGG	1 cut(s)	2117
AccII	CGCG	2 cut(s)	1497, 1923
AciI	CCGC	7 cut(s)	457, 523, 1017, 1495, 1921, 1945, 2061
AcoI	YGGCCR	3 cut(s)	3, 877, 1127
AcsI	RAATTY	2 cut(s)	779, 1253
AcuI	CTGAAG	2 cut(s)	186, 384
AfaI	GTAC	9 cut(s)	317, 449, 1304, 1322, 1346, 1414, 1577, 1859, 2228
AfiI	CCNNNNNNNGG	6 cut(s)	248, 255, 751, 872, 1770, 2117
AflII	CTTAAG	1 cut(s)	929
AjnI	CCWGG	4 cut(s)	879, 1182, 1483, 1546
AleI	CACNNNNGTG	1 cut(s)	1662
Alw21I	GWGCWC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
Alw26I	GTCTC	3 cut(s)	828, 1133, 1454
Alw44I	GTGCAC	1 cut(s)	1270
AlwNI	CAGNNNCTG	1 cut(s)	1553
Ama87I	CYCGRG	3 cut(s)	287, 512, 773
AoxI	GGCC	7 cut(s)	3, 245, 877, 1127, 1486, 2019, 2062
ApaLI	GTGCAC	1 cut(s)	1270
ApeKI	GCWGC	3 cut(s)	30, 2129, 2254
ApoI	RAATTY	2 cut(s)	779, 1253
ArsI	GACNNNNNNTTYG	2 cut(s)	108, 140
AspLEI	GCGC	1 cut(s)	718
AspS9I	GGNCC	3 cut(s)	246, 742, 2063
AsuC2I	CCSGG	4 cut(s)	249, 636, 1735, 2066
AsuHPI	GGTGA	5 cut(s)	59, 952, 1723, 1946, 2132
AvaI	CYCGRG	3 cut(s)	287, 512, 773
AvaII	GGWCC	1 cut(s)	742
BaeGI	GKGCMC	1 cut(s)	1274
BalI	TGGCCA	2 cut(s)	879, 1129
BanI	GGYRCC	1 cut(s)	754
BanII	GRGCYC	3 cut(s)	1629, 1866, 1941
BarI	GAAGNNNNNNTAC	6 cut(s)	1286, 1318, 1841, 1873, 2303, 2335
BbsI	GAAGAC	2 cut(s)	287, 1301
Bbv12I	GWGCWC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
BbvI	GCAGC	3 cut(s)	42, 2116, 2241
BccI	CCATC	4 cut(s)	338, 1098, 1214, 1893
BceAI	ACGGC	2 cut(s)	454, 556
BcgI	CGANNNNNNTGC	2 cut(s)	332, 366
BciT130I	CCWGG	4 cut(s)	881, 1184, 1485, 1548
BclI	TGATCA	1 cut(s)	816
BcnI	CCSGG	4 cut(s)	249, 636, 1735, 2066
BcoDI	GTCTC	3 cut(s)	828, 1133, 1454
BfaI	CTAG	3 cut(s)	224, 629, 1136
BfmI	CTRYAG	2 cut(s)	1213, 1633
BfrI	CTTAAG	1 cut(s)	929
BfuAI	ACCTGC	1 cut(s)	1990
BglII	AGATCT	3 cut(s)	78, 673, 2190
BisI	GCNGC	4 cut(s)	31, 2062, 2130, 2255
BlpI	GCTNAGC	1 cut(s)	708
BlsI	GCNGC	4 cut(s)	32, 2063, 2131, 2256
BmcAI	AGTACT	1 cut(s)	317
Bme1390I	CCNGG	8 cut(s)	249, 636, 881, 1184, 1485, 1548, 1735, 2066
Bme18I	GGWCC	1 cut(s)	742
BmeT110I	CYCGRG	3 cut(s)	287, 512, 773
BmgT120I	GGNCC	3 cut(s)	246, 742, 2063
BmiI	GGNNCC	2 cut(s)	756, 989
BmrFI	CCNGG	8 cut(s)	249, 636, 881, 1184, 1485, 1548, 1735, 2066
BmrI	ACTGGG	1 cut(s)	2191
BmsI	GCATC	6 cut(s)	42, 508, 543, 751, 915, 988
BmuI	ACTGGG	1 cut(s)	2191
BpiI	GAAGAC	2 cut(s)	287, 1301
BpmI	CTGGAG	1 cut(s)	392
Bpu10I	CCTNAGC	1 cut(s)	1769
Bpu1102I	GCTNAGC	1 cut(s)	708
BpuEI	CTTGAG	4 cut(s)	1049, 1096, 1157, 1355
BpuMI	CCSGG	4 cut(s)	249, 636, 1735, 2066
BsaBI	GATNNNNATC	1 cut(s)	2112
BsaJI	CCNNGG	9 cut(s)	242, 634, 798, 829, 1183, 1287, 1546, 1821, 2197
BsaWI	WCCGGW	2 cut(s)	2267, 2343
BsaXI	ACNNNNNCTCC	8 cut(s)	351, 381, 1646, 1676, 1959, 1989, 2177, 2207
Bsc4I	CCNNNNNNNGG	6 cut(s)	248, 255, 751, 872, 1770, 2117
Bse1I	ACTGG	6 cut(s)	56, 503, 877, 1328, 1360, 2186
Bse3DI	GCAATG	4 cut(s)	25, 1275, 1377, 1467
Bse8I	GATNNNNATC	1 cut(s)	2112
BseBI	CCWGG	4 cut(s)	881, 1184, 1485, 1548
BseDI	CCNNGG	9 cut(s)	242, 634, 798, 829, 1183, 1287, 1546, 1821, 2197
BseGI	GGATG	4 cut(s)	264, 499, 580, 1159
BseJI	GATNNNNATC	1 cut(s)	2112
BseLI	CCNNNNNNNGG	6 cut(s)	248, 255, 751, 872, 1770, 2117
BseMI	GCAATG	4 cut(s)	25, 1275, 1377, 1467
BseNI	ACTGG	6 cut(s)	56, 503, 877, 1328, 1360, 2186
BseRI	GAGGAG	2 cut(s)	311, 1847
BseSI	GKGCMC	1 cut(s)	1274
BseXI	GCAGC	3 cut(s)	42, 2116, 2241
BseYI	CCCAGC	2 cut(s)	132, 1799
Bsh1236I	CGCG	2 cut(s)	1497, 1923
BshFI	GGCC	7 cut(s)	5, 247, 879, 1129, 1488, 2021, 2064
BshNI	GGYRCC	1 cut(s)	754
BsiHKAI	GWGCWC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
BsiHKCI	CYCGRG	3 cut(s)	287, 512, 773
BsiSI	CCGG	8 cut(s)	249, 488, 636, 1734, 2066, 2094, 2268, 2344
BslFI	GGGAC	2 cut(s)	1021, 2198
BslI	CCNNNNNNNGG	6 cut(s)	248, 255, 751, 872, 1770, 2117
BsmAI	GTCTC	3 cut(s)	828, 1133, 1454
BsmFI	GGGAC	2 cut(s)	1021, 2198
BsmI	GAATGC	2 cut(s)	969, 1765
BsnI	GGCC	7 cut(s)	5, 247, 879, 1129, 1488, 2021, 2064
BsoBI	CYCGRG	3 cut(s)	287, 512, 773
Bsp1286I	GDGCHC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
Bsp1720I	GCTNAGC	1 cut(s)	708
Bsp19I	CCATGG	1 cut(s)	829
BspACI	CCGC	7 cut(s)	457, 523, 1017, 1495, 1921, 1945, 2061
BspANI	GGCC	7 cut(s)	5, 247, 879, 1129, 1488, 2021, 2064
BspFNI	CGCG	2 cut(s)	1497, 1923
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	2 cut(s)	756, 989
BspMI	ACCTGC	1 cut(s)	1990
BspQI	GCTCTTC	3 cut(s)	240, 482, 1617
BspT107I	GGYRCC	1 cut(s)	754
BspTI	CTTAAG	1 cut(s)	929
BsrDI	GCAATG	4 cut(s)	25, 1275, 1377, 1467
BsrI	ACTGG	6 cut(s)	56, 503, 877, 1328, 1360, 2186
BssECI	CCNNGG	9 cut(s)	242, 634, 798, 829, 1183, 1287, 1546, 1821, 2197
BssT1I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
Bst2UI	CCWGG	4 cut(s)	881, 1184, 1485, 1548
Bst4CI	ACNGT	8 cut(s)	71, 1217, 1447, 1596, 1718, 1893, 1934, 2011
Bst6I	CTCTTC	6 cut(s)	240, 482, 628, 1617, 2214, 2379
BstAFI	CTTAAG	1 cut(s)	929
BstAPI	GCANNNNNTGC	2 cut(s)	667, 761
BstC8I	GCNNGC	5 cut(s)	497, 714, 766, 909, 1190
BstDSI	CCRYGG	1 cut(s)	829
BstF5I	GGATG	4 cut(s)	264, 499, 580, 1159
BstFNI	CGCG	2 cut(s)	1497, 1923
BstHHI	GCGC	1 cut(s)	718
BstMAI	GTCTC	3 cut(s)	828, 1133, 1454
BstMWI	GCNNNNNNNGC	5 cut(s)	667, 713, 761, 1494, 2061
BstNI	CCWGG	4 cut(s)	881, 1184, 1485, 1548
BstNSI	RCATGY	2 cut(s)	499, 2294
BstSCI	CCNGG	8 cut(s)	247, 634, 879, 1182, 1483, 1546, 1733, 2064
BstSFI	CTRYAG	2 cut(s)	1213, 1633
BstSLI	GKGCMC	1 cut(s)	1274
BstUI	CGCG	2 cut(s)	1497, 1923
BstV1I	GCAGC	3 cut(s)	42, 2116, 2241
BstV2I	GAAGAC	2 cut(s)	287, 1301
BstX2I	RGATCY	6 cut(s)	78, 673, 792, 1107, 2136, 2190
BstYI	RGATCY	6 cut(s)	78, 673, 792, 1107, 2136, 2190
BsuRI	GGCC	7 cut(s)	5, 247, 879, 1129, 1488, 2021, 2064
BtgI	CCRYGG	1 cut(s)	829
BtsCI	GGATG	4 cut(s)	264, 499, 580, 1159
BtsIMutI	CAGTG	9 cut(s)	480, 510, 870, 1146, 1222, 1274, 1443, 1578, 2179
BveI	ACCTGC	1 cut(s)	1990
Cac8I	GCNNGC	5 cut(s)	497, 714, 766, 909, 1190
CaiI	CAGNNNCTG	1 cut(s)	1553
CciI	TCATGA	1 cut(s)	535
CfoI	GCGC	1 cut(s)	718
Cfr13I	GGNCC	3 cut(s)	246, 742, 2063
CseI	GACGC	2 cut(s)	1013, 2303
Csp6I	GTAC	9 cut(s)	316, 448, 1303, 1321, 1345, 1413, 1576, 1858, 2227
CspCI	CAANNNNNGTGG	2 cut(s)	1337, 1372
CviQI	GTAC	9 cut(s)	316, 448, 1303, 1321, 1345, 1413, 1576, 1858, 2227
EaeI	YGGCCR	3 cut(s)	3, 877, 1127
Eam1104I	CTCTTC	6 cut(s)	240, 482, 628, 1617, 2214, 2379
EarI	CTCTTC	6 cut(s)	240, 482, 628, 1617, 2214, 2379
EciI	GGCGGA	1 cut(s)	512
Ecl136II	GAGCTC	3 cut(s)	1627, 1864, 1939
Eco130I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
Eco147I	AGGCCT	1 cut(s)	1488
Eco24I	GRGCYC	3 cut(s)	1629, 1866, 1941
Eco32I	GATATC	2 cut(s)	863, 958
Eco47I	GGWCC	1 cut(s)	742
Eco53kI	GAGCTC	3 cut(s)	1627, 1864, 1939
Eco57I	CTGAAG	2 cut(s)	186, 384
Eco88I	CYCGRG	3 cut(s)	287, 512, 773
EcoICRI	GAGCTC	3 cut(s)	1627, 1864, 1939
EcoRII	CCWGG	4 cut(s)	879, 1182, 1483, 1546
EcoRV	GATATC	2 cut(s)	863, 958
EcoT14I	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
EcoT22I	ATGCAT	1 cut(s)	501
EcoT38I	GRGCYC	3 cut(s)	1629, 1866, 1941
ErhI	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
FalI	AAGNNNNNCTT	6 cut(s)	782, 814, 1334, 1366, 1643, 1675
FaqI	GGGAC	2 cut(s)	1021, 2198
FauNDI	CATATG	1 cut(s)	646
FbaI	TGATCA	1 cut(s)	816
Fnu4HI	GCNGC	4 cut(s)	31, 2062, 2130, 2255
FokI	GGATG	4 cut(s)	251, 486, 587, 1166
FriOI	GRGCYC	3 cut(s)	1629, 1866, 1941
Fsp4HI	GCNGC	4 cut(s)	31, 2062, 2130, 2255
FspBI	CTAG	3 cut(s)	224, 629, 1136
GlaI	GCGC	1 cut(s)	717
GluI	GCNGC	4 cut(s)	31, 2062, 2130, 2255
GsaI	CCCAGC	2 cut(s)	136, 1803
GsuI	CTGGAG	1 cut(s)	392
HaeIII	GGCC	7 cut(s)	5, 247, 879, 1129, 1488, 2021, 2064
HapII	CCGG	8 cut(s)	249, 488, 636, 1734, 2066, 2094, 2268, 2344
HgaI	GACGC	2 cut(s)	1013, 2303
HhaI	GCGC	1 cut(s)	718
Hin6I	GCGC	1 cut(s)	716
HinP1I	GCGC	1 cut(s)	716
HincII	GTYRAC	3 cut(s)	1599, 1783, 2146
HindII	GTYRAC	3 cut(s)	1599, 1783, 2146
HindIII	AAGCTT	2 cut(s)	651, 1766
HinfI	GANTC	6 cut(s)	59, 270, 1310, 1462, 1817, 2363
HpaI	GTTAAC	1 cut(s)	1783
HpaII	CCGG	8 cut(s)	249, 488, 636, 1734, 2066, 2094, 2268, 2344
HphI	GGTGA	5 cut(s)	59, 952, 1723, 1946, 2132
Hpy166II	GTNNAC	8 cut(s)	67, 1272, 1365, 1578, 1599, 1783, 2146, 2229
Hpy188I	TCNGA	9 cut(s)	269, 1057, 1079, 1208, 1315, 1898, 1914, 2157, 2248
Hpy8I	GTNNAC	8 cut(s)	67, 1272, 1365, 1578, 1599, 1783, 2146, 2229
Hpy99I	CGWCG	3 cut(s)	344, 1208, 2319
HpyCH4III	ACNGT	8 cut(s)	71, 1217, 1447, 1596, 1718, 1893, 1934, 2011
HpyF10VI	GCNNNNNNNGC	5 cut(s)	667, 713, 761, 1494, 2061
HspAI	GCGC	1 cut(s)	716
Ksp22I	TGATCA	1 cut(s)	816
KspAI	GTTAAC	1 cut(s)	1783
LguI	GCTCTTC	3 cut(s)	240, 482, 1617
LmnI	GCTCC	4 cut(s)	993, 1550, 1654, 1967
Lsp1109I	GCAGC	3 cut(s)	42, 2116, 2241
LweI	GCATC	6 cut(s)	42, 508, 543, 751, 915, 988
MaeI	CTAG	3 cut(s)	224, 629, 1136
MaeIII	GTNAC	2 cut(s)	979, 1261
MflI	RGATCY	6 cut(s)	78, 673, 792, 1107, 2136, 2190
MhlI	GDGCHC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
MlsI	TGGCCA	2 cut(s)	879, 1129
MluCI	AATT	4 cut(s)	779, 1092, 1253, 2073
MluNI	TGGCCA	2 cut(s)	879, 1129
MlyI	GAGTC	4 cut(s)	68, 1456, 1826, 2372
MmeI	TCCRAC	4 cut(s)	27, 151, 357, 1186
Mox20I	TGGCCA	2 cut(s)	879, 1129
Mph1103I	ATGCAT	1 cut(s)	501
MscI	TGGCCA	2 cut(s)	879, 1129
MseI	TTAA	3 cut(s)	807, 930, 1782
MslI	CAYNNNNRTG	6 cut(s)	72, 534, 605, 1382, 1662, 2327
Msp20I	TGGCCA	2 cut(s)	879, 1129
MspA1I	CMGCKG	1 cut(s)	1370
MspCI	CTTAAG	1 cut(s)	929
MspI	CCGG	8 cut(s)	249, 488, 636, 1734, 2066, 2094, 2268, 2344
MspR9I	CCNGG	8 cut(s)	249, 636, 881, 1184, 1485, 1548, 1735, 2066
Mva1269I	GAATGC	2 cut(s)	969, 1765
MvaI	CCWGG	4 cut(s)	881, 1184, 1485, 1548
MvnI	CGCG	2 cut(s)	1497, 1923
MwoI	GCNNNNNNNGC	5 cut(s)	667, 713, 761, 1494, 2061
NciI	CCSGG	4 cut(s)	249, 636, 1735, 2066
NcoI	CCATGG	1 cut(s)	829
NdeI	CATATG	1 cut(s)	646
NlaIV	GGNNCC	2 cut(s)	756, 989
NsiI	ATGCAT	1 cut(s)	501
NspI	RCATGY	2 cut(s)	499, 2294
OliI	CACNNNNGTG	1 cut(s)	1662
PaeI	GCATGC	1 cut(s)	499
PaeR7I	CTCGAG	3 cut(s)	287, 512, 773
PagI	TCATGA	1 cut(s)	535
PceI	AGGCCT	1 cut(s)	1488
PciSI	GCTCTTC	3 cut(s)	240, 482, 1617
PctI	GAATGC	2 cut(s)	969, 1765
PfeI	GAWTC	2 cut(s)	270, 1310
PflFI	GACNNNGTC	2 cut(s)	58, 851
PflMI	CCANNNNNTGG	1 cut(s)	2117
PkrI	GCNGC	4 cut(s)	32, 2063, 2131, 2256
PleI	GAGTC	4 cut(s)	67, 1456, 1825, 2371
PpsI	GAGTC	4 cut(s)	67, 1456, 1825, 2371
Psp124BI	GAGCTC	3 cut(s)	1629, 1866, 1941
Psp6I	CCWGG	4 cut(s)	879, 1182, 1483, 1546
PspFI	CCCAGC	2 cut(s)	132, 1799
PspGI	CCWGG	4 cut(s)	879, 1182, 1483, 1546
PspN4I	GGNNCC	2 cut(s)	756, 989
PspPI	GGNCC	3 cut(s)	246, 742, 2063
PspXI	VCTCGAGB	2 cut(s)	287, 512
PstNI	CAGNNNCTG	1 cut(s)	1553
PsuI	RGATCY	6 cut(s)	78, 673, 792, 1107, 2136, 2190
PsyI	GACNNNGTC	2 cut(s)	58, 851
PvuII	CAGCTG	1 cut(s)	1370
RsaI	GTAC	9 cut(s)	317, 449, 1304, 1322, 1346, 1414, 1577, 1859, 2228
RsaNI	GTAC	9 cut(s)	316, 448, 1303, 1321, 1345, 1413, 1576, 1858, 2227
RseI	CAYNNNNRTG	6 cut(s)	72, 534, 605, 1382, 1662, 2327
SacI	GAGCTC	3 cut(s)	1629, 1866, 1941
SapI	GCTCTTC	3 cut(s)	240, 482, 1617
SaqAI	TTAA	3 cut(s)	807, 930, 1782
SatI	GCNGC	4 cut(s)	31, 2062, 2130, 2255
Sau96I	GGNCC	3 cut(s)	246, 742, 2063
ScaI	AGTACT	1 cut(s)	317
SchI	GAGTC	4 cut(s)	68, 1456, 1826, 2372
ScrFI	CCNGG	8 cut(s)	249, 636, 881, 1184, 1485, 1548, 1735, 2066
SduI	GDGCHC	6 cut(s)	25, 1274, 1629, 1659, 1866, 1941
SfaNI	GCATC	6 cut(s)	42, 508, 543, 751, 915, 988
SfcI	CTRYAG	2 cut(s)	1213, 1633
Sfr274I	CTCGAG	3 cut(s)	287, 512, 773
SinI	GGWCC	1 cut(s)	742
SlaI	CTCGAG	3 cut(s)	287, 512, 773
SmiMI	CAYNNNNRTG	6 cut(s)	72, 534, 605, 1382, 1662, 2327
SmlI	CTYRAG	8 cut(s)	287, 512, 773, 929, 1028, 1111, 1172, 1334
SmoI	CTYRAG	8 cut(s)	287, 512, 773, 929, 1028, 1111, 1172, 1334
SphI	GCATGC	1 cut(s)	499
Sse9I	AATT	4 cut(s)	779, 1092, 1253, 2073
SseBI	AGGCCT	1 cut(s)	1488
SsiI	CCGC	7 cut(s)	457, 523, 1017, 1495, 1921, 1945, 2061
SspI	AATATT	1 cut(s)	1052
SspMI	CTAG	3 cut(s)	224, 629, 1136
SstI	GAGCTC	3 cut(s)	1629, 1866, 1941
StuI	AGGCCT	1 cut(s)	1488
StyD4I	CCNGG	8 cut(s)	247, 634, 879, 1182, 1483, 1546, 1733, 2064
StyI	CCWWGG	6 cut(s)	242, 798, 829, 1287, 1821, 2197
TaaI	ACNGT	8 cut(s)	71, 1217, 1447, 1596, 1718, 1893, 1934, 2011
TaqI	TCGA	9 cut(s)	95, 288, 342, 513, 657, 774, 1521, 1620, 2217
TaqII	GACCGA	1 cut(s)	1409
TasI	AATT	4 cut(s)	779, 1092, 1253, 2073
TatI	WGTACW	3 cut(s)	315, 1320, 2226
TauI	GCSGC	1 cut(s)	2064
TfiI	GAWTC	2 cut(s)	270, 1310
Tru1I	TTAA	3 cut(s)	807, 930, 1782
Tru9I	TTAA	3 cut(s)	807, 930, 1782
TscAI	CASTG	9 cut(s)	487, 510, 877, 1153, 1222, 1274, 1450, 1585, 2186
TseI	GCWGC	3 cut(s)	30, 2129, 2254
TspGWI	ACGGA	2 cut(s)	1055, 1088
TspRI	CASTG	9 cut(s)	487, 510, 877, 1153, 1222, 1274, 1450, 1585, 2186
Tth111I	GACNNNGTC	2 cut(s)	58, 851
Van91I	CCANNNNNTGG	1 cut(s)	2117
Vha464I	CTTAAG	1 cut(s)	929
VneI	GTGCAC	1 cut(s)	1270
VpaK11BI	GGWCC	1 cut(s)	742
XapI	RAATTY	2 cut(s)	779, 1253
XbaI	TCTAGA	1 cut(s)	1135
XceI	RCATGY	2 cut(s)	499, 2294
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1427
XhoI	CTCGAG	3 cut(s)	287, 512, 773
XspI	CTAG	3 cut(s)	224, 629, 1136
ZrmI	AGTACT	1 cut(s)	317
Zsp2I	ATGCAT	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.