Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Reverse (-)

8019656 .. 8024233

Gene Structure 4578 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1100600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGCGGAACGTGTGCTGACCCGCGTCCAGAGCCTCCGGGAGAGGCTGGACGAAACCCTCTCTACTCAACGCAATGATTTTCTGGCCCTGCTTTCTAGGATTGAGAGCAAGGGGAAAGGGTTTCTGCAACCCCATCAGCTTCTTGCCGAGTTCGAAGCGATTCCTGAAAACAACAGGCAGAAGCTGTTGGATGGGGCTTTTGGTGAAGTTTTGAAGCACACCCAGGAGGCTATTGTTTTGCCCCCATGGGTGGCTCTTGCTGTGCGCCCAAGGCCCGGTGTTTGGGAGTACATTCGTGTGAATGTCCATGCTCTCGCTCTCGAGGAGCTGTGTGTGGCTGAGTACTTGCAATTCAAGGAGGAGCTTGTTGATGGAAGTTCCAATGGCAACTTTGTGCTTGAGTTGGACTTTCAACCGTTCAATGCATCTTTCCCCCGCCCAACTCTTTCCAAGTCCATTGGAAACGGCGTTGAGTTCCTCAATCGTCACCTTTCTGCTAAGCTCTTCCATGACAAGGAGAGCCTGCACCCTTTGCTCGAGTTTCTCCGAGTTCACTGCTATGAGGGCAGGAACATGATGCTGAATAACAGAATTAAGAATGTGAATGAGCTGCAACGTGTTCTGAGGAAGGCGGAGGATTTCCTCTCTTCAATTGTCCCCAGAACTCCATACAAGGAGTTCGAGCCCAAGTTGCAAGCCATCGGTTTGGAGAGGGGTTGGGGTGACACTGCAGAGCGCGTGCTTGAGATGATCCAACTTCTGTTGGACCTTCTTGAGGCACCCAATCCTTTTACTCTTGAGAAATTCCTTGGCCAGATCCCCATGGTCTTTAATGTTGTGATCCTTTCGCCCCACGGCTATTTTGCCCAAGACCATGTCTTGGGGTTCCCTGACACTGGTGGCCAGGTTGTCTATATCTTGGATCAAGTTCGTGCTTTGGAGTCTGAGATGCTTAAGCGCATTAAGCAGCAGGGGCTCGATATCACTCCCCGCATTATCATTCTAACAAGGCTTCTTCCTGATGCTGTTGGAACCACATGTGGTGATCGTCTTGAGAAAGTTTACGGGTGTGAGCATTCAGATATTCTTCGTGTTCCTTTCAGAGATAAGAAGGGCGCCATTGTCCGCAGATGGATTTCAAGATTCGAAGTCTGGCCCTACCTAGAGGCATATGCCGAGGACGCGATCAAGGAACTTTCCAAGGAAATGCAGGGCAAGCCTGATCTCATCATTGGAAACTACAGTGATGGCAATATTGTGGCCTCACTGATGGCACACAAATTGGGTGTTACACAGTGCACCATCGCCCATGCTCTGGAGAAGTCAAAATACCCTGATTCTGACCTTTACTGGAAGAAATTGGATGAGAATTATCACTTCTCATGCCAATTTACAGCTGATCTTATCGCCATGAACCACACTGATTTCATCATCACCAGTACCTTCCAAGAGATTGCTGGAAGCAAAGACACTGTTGGCCAGTATGAGAGCCACACTGCCTTCACCCTTCCTGGGCTCTACCGTGTCGTTCATGGTATTGATGTCTTCGATCCCAAGTTCAACATTGTTTCACCAGGAGCTGACATGAATGTCTACTTCCCTTTCTCTGAGAAAGAAAAGAGGTTGACCCACTTCCACCCTGAAATTGAAGAGCTCCTTTACGGCCAAGTCGAAAATATTGAACACTTATGTGTGTTGAAGGACAGGAACAAGCCAATCATCTTCACAATGGCAAGGCTGGACCGGGTGAAGAACATCACCGGGCTTGTCGAGTGGTATGGTAAGAATGCCAAGCTTAGAGAGTTGGTTAACCTTGTTGTGGTTGCTGGTGATCGCCGCAACGAGTCCAAGGACAATGAGGAGAAGGCTGAGTTGAAGAAAATGTATGAGTTGATCGACACCTACAAGCTGAACGGGCAGTTAAGATGGATTTCATCCCAGATGAACCGTGTGAGGAATGGTGAACTCTACCGCTACATTTGTGACACCAGGGGAGCATTTGTGCAGCCTGCTGTGTATGAAGCTTTCGGCCTGACTGTCATTGAGGCCATGACTTGTGGACTGCCCACATTTGCCACTTGCAAGGGTGGCCCGGCTGAGATTATCGTGAACGGAAAATCGGGCTACCACATTGATCCATACCACGGCGATCAGGCTGCTGAGATCCTTGTTGATTTCTTTGAGAAGAACAAGGCTGATCCTAGCCACTGGGACAAGATTTCTCAAGGCGGCTTGCAGCGCATTTACGAGAAGTACACATGGCAAATCTACTCTGAGAGGCTCTTGACACTCACTGGAGTTTATGGCTTCTGGAAAGATGTTTCGAACCTTGATCGCCTGGAGAGCCGCCGCTATCTGGAGATGTTTTACGCCCTCAAGTTCCGCAAGCTCGCGGCGTCTGTTCCTCTTGCTGTGGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

808

Amino Acids

92.57

Weight (kDa)

6.06

Isoelectric Point (pI)

31.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	9 - 120	9.3e-46	Sucrose synthase N-terminal
SUS_EPBD	PF24862	155 - 242	5.2e-33	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	265 - 554	3.7e-164	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	567 - 736	6.8e-27	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	572 - 724	6e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1589
AccB1I	GGYRCC	2 cut(s)	778, 1115
AccB7I	CCANNNNNTGG	2 cut(s)	880, 1315
AccI	GTMKAC	1 cut(s)	1593
AccII	CGCG	4 cut(s)	24, 738, 1184, 2393
AclWI	GGATC	8 cut(s)	745, 811, 835, 930, 1544, 2129, 2158, 2192
AcoI	YGGCCR	4 cut(s)	811, 901, 1477, 1663
AcsI	RAATTY	1 cut(s)	803
AcyI	GRCGYC	2 cut(s)	1116, 2396
AfaI	GTAC	4 cut(s)	290, 344, 1441, 2255
AflII	CTTAAG	1 cut(s)	953
AflIII	ACRYGT	3 cut(s)	10, 616, 1037
AjnI	CCWGG	6 cut(s)	222, 903, 1510, 1573, 1988, 2337
AleI	CACNNNNGTG	1 cut(s)	1689
Alw21I	GWGCWC	2 cut(s)	1301, 1656
Alw44I	GTGCAC	1 cut(s)	1297
AlwI	GGATC	8 cut(s)	745, 811, 835, 930, 1544, 2129, 2158, 2192
AlwNI	CAGNNNCTG	2 cut(s)	184, 1580
Ama87I	CYCGRG	2 cut(s)	320, 536
ApaLI	GTGCAC	1 cut(s)	1297
ApeKI	GCWGC	5 cut(s)	610, 967, 2005, 2156, 2236
ApoI	RAATTY	1 cut(s)	803
Asp700I	GAANNNNTTC	1 cut(s)	159
AspLEI	GCGC	5 cut(s)	267, 738, 960, 1118, 2241
AspS9I	GGNCC	6 cut(s)	85, 273, 766, 1155, 1741, 2090
AsuC2I	CCSGG	5 cut(s)	38, 276, 1745, 1762, 2093
AsuII	TTCGAA	3 cut(s)	153, 1146, 2324
AvaI	CYCGRG	2 cut(s)	320, 536
AvaII	GGWCC	2 cut(s)	766, 1741
BaeGI	GKGCMC	1 cut(s)	1301
BalI	TGGCCA	3 cut(s)	813, 903, 1479
BanI	GGYRCC	2 cut(s)	778, 1115
BanII	GRGCYC	4 cut(s)	687, 978, 1518, 1656
BarI	GAAGNNNNNNTAC	2 cut(s)	1313, 1345
BbsI	GAAGAC	1 cut(s)	1537
Bbv12I	GWGCWC	2 cut(s)	1301, 1656
BbvI	GCAGC	5 cut(s)	597, 979, 2017, 2143, 2248
BccI	CCATC	9 cut(s)	141, 185, 365, 707, 1125, 1241, 1264, 1310, 1920
BceAI	ACGGC	4 cut(s)	481, 871, 1678, 2161
BcgI	CGANNNNNNTGC	4 cut(s)	537, 571, 2138, 2172
BciT130I	CCWGG	6 cut(s)	224, 905, 1512, 1575, 1990, 2339
BcnI	CCSGG	5 cut(s)	38, 276, 1745, 1762, 2093
BfaI	CTAG	3 cut(s)	96, 1163, 2202
BfmI	CTRYAG	2 cut(s)	729, 1240
BfoI	RGCGCY	1 cut(s)	1119
BfrI	CTTAAG	1 cut(s)	953
BlpI	GCTNAGC	1 cut(s)	498
BmcAI	AGTACT	1 cut(s)	344
Bme18I	GGWCC	2 cut(s)	766, 1741
BmeT110I	CYCGRG	2 cut(s)	320, 536
BmgT120I	GGNCC	6 cut(s)	85, 273, 766, 1155, 1741, 2090
BmiI	GGNNCC	4 cut(s)	780, 887, 1033, 1117
BmrI	ACTGGG	1 cut(s)	2218
BmsI	GCATC	4 cut(s)	434, 567, 939, 1012
BmuI	ACTGGG	1 cut(s)	2218
BoxI	GACNNNNGTC	1 cut(s)	23
BpiI	GAAGAC	1 cut(s)	1537
BpmI	CTGGAG	4 cut(s)	1337, 2316, 2360, 2378
Bpu1102I	GCTNAGC	1 cut(s)	498
Bpu14I	TTCGAA	3 cut(s)	153, 1146, 2324
BpuEI	CTTGAG	7 cut(s)	419, 764, 794, 818, 1073, 2208, 2360
BpuMI	CCSGG	5 cut(s)	38, 276, 1745, 1762, 2093
BsaHI	GRCGYC	2 cut(s)	1116, 2396
BsaXI	ACNNNNNCTCC	2 cut(s)	1986, 2016
Bse1I	ACTGG	6 cut(s)	901, 1355, 1437, 1480, 2213, 2299
Bse3DI	GCAATG	1 cut(s)	79
BseBI	CCWGG	6 cut(s)	224, 905, 1512, 1575, 1990, 2339
BseGI	GGATG	3 cut(s)	196, 1369, 1934
BseMI	GCAATG	1 cut(s)	79
BseMII	CTCAG	8 cut(s)	330, 614, 936, 1599, 1860, 2088, 2151, 2265
BseNI	ACTGG	6 cut(s)	901, 1355, 1437, 1480, 2213, 2299
BseRI	GAGGAG	3 cut(s)	338, 374, 1874
BseSI	GKGCMC	1 cut(s)	1301
BseXI	GCAGC	5 cut(s)	597, 979, 2017, 2143, 2248
BsgI	GTGCAG	2 cut(s)	509, 2024
Bsh1236I	CGCG	4 cut(s)	24, 738, 1184, 2393
BshNI	GGYRCC	2 cut(s)	778, 1115
BsiHKAI	GWGCWC	2 cut(s)	1301, 1656
BsiHKCI	CYCGRG	2 cut(s)	320, 536
BsiSI	CCGG	5 cut(s)	37, 276, 1744, 1761, 2093
BslFI	GGGAC	2 cut(s)	641, 2225
BsmFI	GGGAC	2 cut(s)	641, 2225
BsmI	GAATGC	2 cut(s)	1075, 1792
BsoBI	CYCGRG	2 cut(s)	320, 536
Bsp119I	TTCGAA	3 cut(s)	153, 1146, 2324
Bsp1286I	GDGCHC	5 cut(s)	687, 978, 1301, 1518, 1656
Bsp1720I	GCTNAGC	1 cut(s)	498
Bsp19I	CCATGG	2 cut(s)	245, 822
BspCNI	CTCAG	8 cut(s)	331, 615, 937, 1600, 1861, 2089, 2152, 2266
BspFNI	CGCG	4 cut(s)	24, 738, 1184, 2393
BspLI	GGNNCC	4 cut(s)	780, 887, 1033, 1117
BspMAI	CTGCAG	1 cut(s)	733
BspPI	GGATC	8 cut(s)	745, 811, 835, 930, 1544, 2129, 2158, 2192
BspQI	GCTCTTC	2 cut(s)	509, 1644
BspT104I	TTCGAA	3 cut(s)	153, 1146, 2324
BspT107I	GGYRCC	2 cut(s)	778, 1115
BspTI	CTTAAG	1 cut(s)	953
BsrDI	GCAATG	1 cut(s)	79
BsrI	ACTGG	6 cut(s)	901, 1355, 1437, 1480, 2213, 2299
BssNI	GRCGYC	2 cut(s)	1116, 2396
BssT1I	CCWWGG	6 cut(s)	245, 269, 808, 822, 1200, 1848
Bst2UI	CCWGG	6 cut(s)	224, 905, 1512, 1575, 1990, 2339
Bst4CI	ACNGT	7 cut(s)	417, 1244, 1296, 1474, 1523, 1949, 2038
Bst6I	CTCTTC	3 cut(s)	509, 652, 1644
BstACI	GRCGYC	2 cut(s)	1116, 2396
BstAFI	CTTAAG	1 cut(s)	953
BstAPI	GCANNNNNTGC	1 cut(s)	532
BstBI	TTCGAA	3 cut(s)	153, 1146, 2324
BstC8I	GCNNGC	8 cut(s)	524, 696, 740, 1217, 2010, 2234, 2387, 2391
BstDSI	CCRYGG	4 cut(s)	245, 822, 853, 2143
BstENI	CCTNNNNNAGG	1 cut(s)	773
BstF5I	GGATG	3 cut(s)	196, 1369, 1934
BstFNI	CGCG	4 cut(s)	24, 738, 1184, 2393
BstH2I	RGCGCY	1 cut(s)	1119
BstHHI	GCGC	5 cut(s)	267, 738, 960, 1118, 2241
BstNI	CCWGG	6 cut(s)	224, 905, 1512, 1575, 1990, 2339
BstNSI	RCATGY	1 cut(s)	1041
BstPAI	GACNNNNGTC	1 cut(s)	23
BstSFI	CTRYAG	2 cut(s)	729, 1240
BstSLI	GKGCMC	1 cut(s)	1301
BstUI	CGCG	4 cut(s)	24, 738, 1184, 2393
BstV1I	GCAGC	5 cut(s)	597, 979, 2017, 2143, 2248
BstV2I	GAAGAC	1 cut(s)	1537
BstX2I	RGATCY	2 cut(s)	816, 2163
BstXI	CCANNNNNNTGG	1 cut(s)	706
BstYI	RGATCY	2 cut(s)	816, 2163
BtgI	CCRYGG	4 cut(s)	245, 822, 853, 2143
BtgZI	GCGATG	1 cut(s)	1288
BtsCI	GGATG	3 cut(s)	196, 1369, 1934
BtsI	GCAGTG	3 cut(s)	553, 726, 1494
Cac8I	GCNNGC	8 cut(s)	524, 696, 740, 1217, 2010, 2234, 2387, 2391
CaiI	CAGNNNCTG	2 cut(s)	184, 1580
CfoI	GCGC	5 cut(s)	267, 738, 960, 1118, 2241
Cfr13I	GGNCC	6 cut(s)	85, 273, 766, 1155, 1741, 2090
CseI	GACGC	3 cut(s)	13, 1190, 2385
Csp6I	GTAC	4 cut(s)	289, 343, 1440, 2254
CviQI	GTAC	4 cut(s)	289, 343, 1440, 2254
DinI	GGCGCC	1 cut(s)	1117
DrdI	GACNNNNNNGTC	1 cut(s)	1589
DseDI	GACNNNNNNGTC	1 cut(s)	1589
EaeI	YGGCCR	4 cut(s)	811, 901, 1477, 1663
Eam1104I	CTCTTC	3 cut(s)	509, 652, 1644
EarI	CTCTTC	3 cut(s)	509, 652, 1644
EciI	GGCGGA	2 cut(s)	20, 647
Ecl136II	GAGCTC	1 cut(s)	1654
Eco130I	CCWWGG	6 cut(s)	245, 269, 808, 822, 1200, 1848
Eco24I	GRGCYC	4 cut(s)	687, 978, 1518, 1656
Eco32I	GATATC	1 cut(s)	982
Eco47I	GGWCC	2 cut(s)	766, 1741
Eco53kI	GAGCTC	1 cut(s)	1654
Eco88I	CYCGRG	2 cut(s)	320, 536
EcoICRI	GAGCTC	1 cut(s)	1654
EcoNI	CCTNNNNNAGG	1 cut(s)	773
EcoRII	CCWGG	6 cut(s)	222, 903, 1510, 1573, 1988, 2337
EcoRV	GATATC	1 cut(s)	982
EcoT14I	CCWWGG	6 cut(s)	245, 269, 808, 822, 1200, 1848
EcoT22I	ATGCAT	1 cut(s)	427
EcoT38I	GRGCYC	4 cut(s)	687, 978, 1518, 1656
EgeI	GGCGCC	1 cut(s)	1117
EheI	GGCGCC	1 cut(s)	1117
ErhI	CCWWGG	6 cut(s)	245, 269, 808, 822, 1200, 1848
FalI	AAGNNNNNCTT	2 cut(s)	1641, 1673
FaqI	GGGAC	2 cut(s)	641, 2225
FauI	CCCGC	3 cut(s)	29, 443, 998
FauNDI	CATATG	1 cut(s)	1171
FblI	GTMKAC	1 cut(s)	1593
FokI	GGATG	3 cut(s)	203, 1376, 1921
FriOI	GRGCYC	4 cut(s)	687, 978, 1518, 1656
FspBI	CTAG	3 cut(s)	96, 1163, 2202
GlaI	GCGC	5 cut(s)	266, 737, 959, 1117, 2240
GsuI	CTGGAG	4 cut(s)	1337, 2316, 2360, 2378
HaeII	RGCGCY	1 cut(s)	1119
HapII	CCGG	5 cut(s)	37, 276, 1744, 1761, 2093
HgaI	GACGC	3 cut(s)	13, 1190, 2385
HhaI	GCGC	5 cut(s)	267, 738, 960, 1118, 2241
Hin1I	GRCGYC	2 cut(s)	1116, 2396
Hin6I	GCGC	5 cut(s)	265, 736, 958, 1116, 2239
HinP1I	GCGC	5 cut(s)	265, 736, 958, 1116, 2239
HincII	GTYRAC	2 cut(s)	1626, 1810
HindII	GTYRAC	2 cut(s)	1626, 1810
HindIII	AAGCTT	2 cut(s)	1793, 2022
HinfI	GANTC	5 cut(s)	160, 941, 1143, 1337, 1844
HpaI	GTTAAC	1 cut(s)	1810
HpaII	CCGG	5 cut(s)	37, 276, 1744, 1761, 2093
Hpy188I	TCNGA	8 cut(s)	548, 624, 946, 1081, 1103, 1342, 1609, 2275
HpyAV	CCTTC	8 cut(s)	622, 779, 1105, 1453, 1510, 1517, 1693, 1858
HpyCH4III	ACNGT	7 cut(s)	417, 1244, 1296, 1474, 1523, 1949, 2038
HpyCH4IV	ACGT	2 cut(s)	10, 616
HpySE526I	ACGT	2 cut(s)	10, 616
Hsp92I	GRCGYC	2 cut(s)	1116, 2396
HspAI	GCGC	5 cut(s)	265, 736, 958, 1116, 2239
KasI	GGCGCC	1 cut(s)	1115
KspAI	GTTAAC	1 cut(s)	1810
LguI	GCTCTTC	2 cut(s)	509, 1644
LmnI	GCTCC	5 cut(s)	325, 361, 1577, 1659, 1994
Lsp1109I	GCAGC	5 cut(s)	597, 979, 2017, 2143, 2248
LweI	GCATC	4 cut(s)	434, 567, 939, 1012
MaeI	CTAG	3 cut(s)	96, 1163, 2202
MaeII	ACGT	2 cut(s)	10, 616
MaeIII	GTNAC	4 cut(s)	485, 722, 1288, 1982
MfeI	CAATTG	1 cut(s)	651
MflI	RGATCY	2 cut(s)	816, 2163
MhlI	GDGCHC	5 cut(s)	687, 978, 1301, 1518, 1656
MlsI	TGGCCA	3 cut(s)	813, 903, 1479
MluCI	AATT	9 cut(s)	350, 591, 651, 803, 1280, 1358, 1369, 1388, 1644
MluNI	TGGCCA	3 cut(s)	813, 903, 1479
Mly113I	GGCGCC	1 cut(s)	1116
MlyI	GAGTC	2 cut(s)	950, 1853
MmeI	TCCRAC	5 cut(s)	168, 384, 744, 778, 1009
Mox20I	TGGCCA	3 cut(s)	813, 903, 1479
Mph1103I	ATGCAT	1 cut(s)	427
MroXI	GAANNNNTTC	1 cut(s)	159
MscI	TGGCCA	3 cut(s)	813, 903, 1479
MseI	TTAA	6 cut(s)	594, 831, 954, 963, 1809, 1922
MslI	CAYNNNNRTG	3 cut(s)	296, 558, 1689
Msp20I	TGGCCA	3 cut(s)	813, 903, 1479
MspA1I	CMGCKG	1 cut(s)	1397
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	5 cut(s)	37, 276, 1744, 1761, 2093
MunI	CAATTG	1 cut(s)	651
Mva1269I	GAATGC	2 cut(s)	1075, 1792
MvaI	CCWGG	6 cut(s)	224, 905, 1512, 1575, 1990, 2339
MvnI	CGCG	4 cut(s)	24, 738, 1184, 2393
NarI	GGCGCC	1 cut(s)	1116
NciI	CCSGG	5 cut(s)	38, 276, 1745, 1762, 2093
NcoI	CCATGG	2 cut(s)	245, 822
NdeI	CATATG	1 cut(s)	1171
NlaIV	GGNNCC	4 cut(s)	780, 887, 1033, 1117
NmeAIII	GCCGAG	2 cut(s)	172, 1201
NmuCI	GTSAC	3 cut(s)	485, 722, 1982
NsiI	ATGCAT	1 cut(s)	427
NspI	RCATGY	1 cut(s)	1041
NspV	TTCGAA	3 cut(s)	153, 1146, 2324
OliI	CACNNNNGTG	1 cut(s)	1689
PaeR7I	CTCGAG	2 cut(s)	320, 536
PciI	ACATGT	1 cut(s)	1037
PciSI	GCTCTTC	2 cut(s)	509, 1644
PcsI	WCGNNNNNNNCGW	1 cut(s)	1668
PctI	GAATGC	2 cut(s)	1075, 1792
PdmI	GAANNNNTTC	1 cut(s)	159
PfeI	GAWTC	3 cut(s)	160, 1143, 1337
PflFI	GACNNNGTC	1 cut(s)	875
PflMI	CCANNNNNTGG	2 cut(s)	880, 1315
PfoI	TCCNGGA	1 cut(s)	36
PleI	GAGTC	2 cut(s)	949, 1852
PluTI	GGCGCC	1 cut(s)	1119
PpsI	GAGTC	2 cut(s)	949, 1852
PscI	ACATGT	1 cut(s)	1037
PshAI	GACNNNNGTC	1 cut(s)	23
Psp124BI	GAGCTC	1 cut(s)	1656
Psp6I	CCWGG	6 cut(s)	222, 903, 1510, 1573, 1988, 2337
PspGI	CCWGG	6 cut(s)	222, 903, 1510, 1573, 1988, 2337
PspN4I	GGNNCC	4 cut(s)	780, 887, 1033, 1117
PspPI	GGNCC	6 cut(s)	85, 273, 766, 1155, 1741, 2090
PspXI	VCTCGAGB	1 cut(s)	536
PstI	CTGCAG	1 cut(s)	733
PstNI	CAGNNNCTG	2 cut(s)	184, 1580
PsuI	RGATCY	2 cut(s)	816, 2163
PsyI	GACNNNGTC	1 cut(s)	875
PvuII	CAGCTG	1 cut(s)	1397
RsaI	GTAC	4 cut(s)	290, 344, 1441, 2255
RsaNI	GTAC	4 cut(s)	289, 343, 1440, 2254
RseI	CAYNNNNRTG	3 cut(s)	296, 558, 1689
SacI	GAGCTC	1 cut(s)	1656
SapI	GCTCTTC	2 cut(s)	509, 1644
SaqAI	TTAA	6 cut(s)	594, 831, 954, 963, 1809, 1922
Sau96I	GGNCC	6 cut(s)	85, 273, 766, 1155, 1741, 2090
ScaI	AGTACT	1 cut(s)	344
SchI	GAGTC	2 cut(s)	950, 1853
SduI	GDGCHC	5 cut(s)	687, 978, 1301, 1518, 1656
SfaNI	GCATC	4 cut(s)	434, 567, 939, 1012
SfcI	CTRYAG	2 cut(s)	729, 1240
SfoI	GGCGCC	1 cut(s)	1117
Sfr274I	CTCGAG	2 cut(s)	320, 536
SfuI	TTCGAA	3 cut(s)	153, 1146, 2324
SinI	GGWCC	2 cut(s)	766, 1741
SlaI	CTCGAG	2 cut(s)	320, 536
SmiMI	CAYNNNNRTG	3 cut(s)	296, 558, 1689
Sse9I	AATT	9 cut(s)	350, 591, 651, 803, 1280, 1358, 1369, 1388, 1644
SspDI	GGCGCC	1 cut(s)	1115
SspI	AATATT	2 cut(s)	1255, 1678
SspMI	CTAG	3 cut(s)	96, 1163, 2202
SstI	GAGCTC	1 cut(s)	1656
StyI	CCWWGG	6 cut(s)	245, 269, 808, 822, 1200, 1848
TaaI	ACNGT	7 cut(s)	417, 1244, 1296, 1474, 1523, 1949, 2038
TaiI	ACGT	2 cut(s)	13, 619
TasI	AATT	9 cut(s)	350, 591, 651, 803, 1280, 1358, 1369, 1388, 1644
TatI	WGTACW	3 cut(s)	288, 342, 2253
TauI	GCSGC	5 cut(s)	1839, 2232, 2349, 2352, 2396
TfiI	GAWTC	3 cut(s)	160, 1143, 1337
Tru1I	TTAA	6 cut(s)	594, 831, 954, 963, 1809, 1922
Tru9I	TTAA	6 cut(s)	594, 831, 954, 963, 1809, 1922
TseFI	GTSAC	3 cut(s)	485, 722, 1982
TseI	GCWGC	5 cut(s)	610, 967, 2005, 2156, 2236
Tsp45I	GTSAC	3 cut(s)	485, 722, 1982
TspDTI	ATGAA	7 cut(s)	1417, 1427, 1520, 1601, 1923, 1958, 2034
TspGWI	ACGGA	1 cut(s)	2127
Tth111I	GACNNNGTC	1 cut(s)	875
Van91I	CCANNNNNTGG	2 cut(s)	880, 1315
Vha464I	CTTAAG	1 cut(s)	953
VneI	GTGCAC	1 cut(s)	1297
VpaK11BI	GGWCC	2 cut(s)	766, 1741
XagI	CCTNNNNNAGG	1 cut(s)	773
XapI	RAATTY	1 cut(s)	803
XceI	RCATGY	1 cut(s)	1041
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1454
XhoI	CTCGAG	2 cut(s)	320, 536
XmiI	GTMKAC	1 cut(s)	1593
XmnI	GAANNNNTTC	1 cut(s)	159
XspI	CTAG	3 cut(s)	96, 1163, 2202
ZrmI	AGTACT	1 cut(s)	344
Zsp2I	ATGCAT	1 cut(s)	427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.