Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001556

Physical Location & Seq

Genomic Coordinates Forward (+)

6548921 .. 6551789

Gene Structure 2869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G006750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2349 bp

ATGGCCGATCCTTCTTCATGTGCTCATTGCAGCATCTATGAGAGGTTGGACTGGACTGAGTCTATTCACCGTGATGAGATCTTGGCTGTGCTTTCGAGGGTTGAAAGCAAGGGCAAAGGGTTTGTCCAACCCCAGCAACTTGTTGCTGGGTTTGAAGCAATCCCTGAAGCATTTGGAGAAGTTTCAAAGAACATTCAGGAAGCTGTTGTTCTATCTCCATACCTAGCCCTCGCTCTTCGTCCAAGGCCCGGTGTTTGGGAACACATCCGAATCAACATGAAGACACTCGAGGTTGAGGAGTTGCCTGTGGCTGAGTACTTGAAGATGAAGGAGGAACTCGTCGATGGAAGTGCCAATGGAGGCTTTGTGCTGGAGTTGGACTTTGAGCCATTCAATGCTTCAGTTCCCAAACCAACTCTTTCCAAGTCCATTGGAAAAGGCGTTGAGTACCTCAACCGCCACCTCTCTGCAAAGCTCTTCACTGAGCCGGAGAGCATGCATCCAGTGTTGCTCGAGTTTCTCCGCCTTCACACTCATGACGGCAAGAACCTGATGCTGAATGACAACATTGAGGATGTGGAAGAACTCAAACATGTTCTCAGGGAGGAGGTGGGGAAGTATCTCTCTTCACTAGCCCCCGGAACTCCATATGAAGCTTTCGAGCATAAGTTGCAAGATCTTGGCTTGGAGAGAGGTTGGGGAAACAATGCTGAGCGTGTGCTTGAAATGATCCAGCTTCTCTTGGACCTTCTTGATTCACCAGATGCTTGCAATCTCGAGAAATTCATCAGCAAGATCCCCTTGGTCTTTAATGTTGTGATCATGTCTCCCCATGGTTATTTTGCACAAGACAATGTCTTGGGATATCCTGACACTGGTGGCCAGGTTGTTTATATCTTGGATCAAGTGCGTGCTTTGGAGAATGAGATGCTTAAGCGTATCAAGGAACAAGGGCTTGATATCACCCCTCGCATTCTTATTGTTACAAGGCTCCTTCCTGATGCTGTCGGGACTACTTGCGGTCAGCGTCTTGAGAGGGTGTATGGAACTGAATATTCGGATATTATCCGTGTTCCTTTCAGAACAGAGAAGGGAATTGTCCGTAGATGGATCTCAAGATTTGAAGTCTGGCCATATCTAGAGACTTACACTGAGGATGTTGCAACTGAACTTCTCAAGGAGTTCCAGGGCAAGCCTGATCTTATCGTCGGAAACTACAGTGATGGAAACATTGTGGCCTCATTGTTGGCACACAAATTTGATGTTACTCAGTGCACCATTGCTCATGCCTTGGAGAAGACAAAGTACCCTGATTCTGACTTGTACTGGAAGAAACTTGAGGACAAGTACCACTTCTCGTGCCAGTTCACAGCTGATCTCATTGCCATGAACCAGACCGATTTCATTATCACAAGTACCTTCCAAGAGATTGCTGGAAGCAAAGACACTGTTGGTCAATATGAGACTCACATTGCCTTCACAATGCCTGGCCTCTACCGCGTTGTCAATGGTATAGATTGCTTCGATCCCAAGTTCAACATTGTTTCCCCAGGAGCTGACATGGACATTTACTTCCCGTACACTGAGAAGGAAAGACGGTTGACATCATTCCACCCCGAAATTGAAGAGCTCATCTACAGCCAACCTATCATCTTCACAATGGCAAGGCTTGACCGTGTGAAGAACATCACCGGGCTTGTGGAATGGTATGGCAAGAATGCCAAGCTTAGGGAGTTGGTTAACCTTGTGGTTGTGGCTGGGGATCGGCGAAAGGAGTCCAAGGACAACGAGGAGAAGGCTGAGATGAAGAAGATGTACGAGCTCATTGACACCTACAAGTTGAATGGACAGTTCAGATGGATTTCTTCTCAGATGAACCGCGTGAGGAACGGTGAGCTCTACCGCTATATTGCTGACACTAAAGGAGCTTTTGTGCAACCTGCTGTCTATGAAGCCTTTGGCTTGACTGTTGTGGAGGCCATGACTTGTGGATTGCCTACCTTTGCTACTTGCAAAGGCGGCCCGGCGGAGATTATTGTGAATGGCAAATCCGGCTACCACATTGATCCATATCATGGTGACAAAGCTGCTGAGATCCTTGTTGACTTCTTTCAGAAGTGCAAGGTTGATCCTTCTCACTGGGACAAGATCTCCCAAGGAGGCTTGAAGCGTATCGAAGAGAAGTATACTTGGCAGATTTACTCTGAGAGGCTGCTGACCCTGACCGGAGTTTATGGCTTCTGGAAGCATGTTTCTAACCTTGACGAGAAGCGTCGCTACATAGAAATGTTGTATGCCCTCCGGTATAAGAAATCGGTTGAGTCTGTTCCTCTTGCTGAAGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0001666 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008194 GO:0009266 GO:0009409 GO:0009413 GO:0009414 GO:0009415 GO:0009506 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010037 GO:0010038 GO:0010154 GO:0010431 GO:0010555 GO:0016020 GO:0016043 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0021700 GO:0022414 GO:0022607 GO:0030054 GO:0032501 GO:0032502 GO:0032504 GO:0034059 GO:0034284 GO:0034285 GO:0035251 GO:0036293 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0046686 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051259 GO:0051262 GO:0055044 GO:0061458 GO:0065003 GO:0070482 GO:0071695 GO:0071840 GO:0071944 GO:0072347 GO:0072708 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

782

Amino Acids

89.39

Weight (kDa)

5.38

Isoelectric Point (pI)

39.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	11 - 111	1.8e-28	Sucrose synthase N-terminal
SUS_EPBD	PF24862	146 - 235	1.6e-28	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	258 - 546	3.4e-166	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	547 - 700	5.1e-25	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	549 - 701	7.2e-11	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43190 AT3G43190 AT5G20830 AT5G20830 AT5G20830
fragaria_vesca	FvH4_1g07260 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360 FvH4_1g09360
malus_domestica	MD02G1100500.v1.1 MD02G1100600.v1.1 MD15G1223500.v1.1
prunus_persica	Prupe.2G242300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1 Prupe.7G192300_v2.0.a1
pyrus_communis	pycom02g07890 pycom15g19770
rosa_chinensis	RchiOBHm_Chr2g0093161 RchiOBHm_Chr2g0095901
rosa_laevigata	RLG00000016373 RLG00000016605
rosa_multiflora	Rmu_sc0003724.1_g000005 Rmu_sc0014241.1_g000002 Rmu_sc0038852.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145980 Rroxscaffold_2G00148460
rosa_rugosa	Rorug02G0033300 Rorug02G0053600 Rorug02G0053600 Rorug02G0053700 Rorug02G0053800
rosa_samantha	Rh2AG079000 Rh2AG102900 Rh2BG080300 Rh2BG104300 Rh2CG082500 Rh2CG106800 Rh2DG078000 Rh2DG104500
rosa_wichuraiana	Rw2G006750 Rw2G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1948
AccB7I	CCANNNNNTGG	1 cut(s)	2075
AccI	GTMKAC	1 cut(s)	2186
AccII	CGCG	2 cut(s)	1500, 1881
AciI	CCGC	8 cut(s)	457, 523, 1020, 1498, 1879, 1903, 2019, 2027
AcoI	YGGCCR	3 cut(s)	3, 880, 1130
AcsI	RAATTY	2 cut(s)	782, 1256
AcuI	CTGAAG	2 cut(s)	186, 384
AfaI	GTAC	8 cut(s)	317, 449, 1307, 1325, 1349, 1417, 1580, 1817
AfiI	CCNNNNNNNGG	5 cut(s)	248, 255, 875, 1728, 2075
AflII	CTTAAG	1 cut(s)	932
AflIII	ACRYGT	1 cut(s)	592
AjnI	CCWGG	4 cut(s)	882, 1185, 1486, 1549
Alw21I	GWGCWC	5 cut(s)	25, 1277, 1632, 1824, 1899
Alw26I	GTCTC	3 cut(s)	831, 1136, 1457
Alw44I	GTGCAC	1 cut(s)	1273
AlwNI	CAGNNNCTG	1 cut(s)	1556
Ama87I	CYCGRG	3 cut(s)	287, 512, 776
AoxI	GGCC	8 cut(s)	3, 245, 880, 1130, 1236, 1489, 1977, 2020
ApaLI	GTGCAC	1 cut(s)	1273
ApeKI	GCWGC	3 cut(s)	30, 2087, 2212
ApoI	RAATTY	2 cut(s)	782, 1256
ArsI	GACNNNNNNTTYG	2 cut(s)	108, 140
AspS9I	GGNCC	3 cut(s)	246, 745, 2021
AsuC2I	CCSGG	4 cut(s)	249, 639, 1693, 2024
AsuHPI	GGTGA	6 cut(s)	59, 750, 955, 1681, 1904, 2090
AvaI	CYCGRG	3 cut(s)	287, 512, 776
AvaII	GGWCC	1 cut(s)	745
BaeGI	GKGCMC	1 cut(s)	1277
BalI	TGGCCA	2 cut(s)	882, 1132
BanII	GRGCYC	3 cut(s)	1632, 1824, 1899
BarI	GAAGNNNNNNTAC	6 cut(s)	1289, 1321, 1799, 1831, 2261, 2293
BauI	CACGAG	1 cut(s)	1357
BbsI	GAAGAC	2 cut(s)	287, 1304
Bbv12I	GWGCWC	5 cut(s)	25, 1277, 1632, 1824, 1899
BbvI	GCAGC	3 cut(s)	42, 2074, 2199
BccI	CCATC	4 cut(s)	338, 1101, 1217, 1851
BceAI	ACGGC	1 cut(s)	556
BcgI	CGANNNNNNTGC	2 cut(s)	332, 366
BciT130I	CCWGG	4 cut(s)	884, 1187, 1488, 1551
BclI	TGATCA	1 cut(s)	819
BcnI	CCSGG	4 cut(s)	249, 639, 1693, 2024
BcoDI	GTCTC	3 cut(s)	831, 1136, 1457
BfaI	CTAG	3 cut(s)	224, 632, 1139
BfmI	CTRYAG	2 cut(s)	1216, 1636
BfrI	CTTAAG	1 cut(s)	932
BfuAI	ACCTGC	1 cut(s)	1948
BglII	AGATCT	3 cut(s)	78, 676, 2148
BisI	GCNGC	4 cut(s)	31, 2020, 2088, 2213
BlpI	GCTNAGC	1 cut(s)	711
BlsI	GCNGC	4 cut(s)	32, 2021, 2089, 2214
BmcAI	AGTACT	1 cut(s)	317
Bme1390I	CCNGG	8 cut(s)	249, 639, 884, 1187, 1488, 1551, 1693, 2024
Bme18I	GGWCC	1 cut(s)	745
BmeT110I	CYCGRG	3 cut(s)	287, 512, 776
BmgT120I	GGNCC	3 cut(s)	246, 745, 2021
BmiI	GGNNCC	1 cut(s)	992
BmrFI	CCNGG	8 cut(s)	249, 639, 884, 1187, 1488, 1551, 1693, 2024
BmrI	ACTGGG	1 cut(s)	2149
BmsI	GCATC	6 cut(s)	42, 508, 543, 754, 918, 991
BmuI	ACTGGG	1 cut(s)	2149
BpiI	GAAGAC	2 cut(s)	287, 1304
BpmI	CTGGAG	1 cut(s)	392
Bpu10I	CCTNAGC	1 cut(s)	1727
Bpu1102I	GCTNAGC	1 cut(s)	711
BpuEI	CTTGAG	4 cut(s)	1052, 1099, 1160, 1358
BpuMI	CCSGG	4 cut(s)	249, 639, 1693, 2024
BsaBI	GATNNNNATC	1 cut(s)	2070
BsaJI	CCNNGG	9 cut(s)	242, 637, 801, 832, 1186, 1290, 1549, 1779, 2155
BsaWI	WCCGGW	2 cut(s)	2225, 2301
BsaXI	ACNNNNNCTCC	6 cut(s)	351, 381, 1917, 1947, 2135, 2165
Bsc4I	CCNNNNNNNGG	5 cut(s)	248, 255, 875, 1728, 2075
Bse1I	ACTGG	6 cut(s)	56, 503, 880, 1331, 1363, 2144
Bse3DI	GCAATG	4 cut(s)	25, 1278, 1380, 1470
Bse8I	GATNNNNATC	1 cut(s)	2070
BseBI	CCWGG	4 cut(s)	884, 1187, 1488, 1551
BseDI	CCNNGG	9 cut(s)	242, 637, 801, 832, 1186, 1290, 1549, 1779, 2155
BseGI	GGATG	4 cut(s)	264, 499, 580, 1162
BseJI	GATNNNNATC	1 cut(s)	2070
BseLI	CCNNNNNNNGG	5 cut(s)	248, 255, 875, 1728, 2075
BseMI	GCAATG	4 cut(s)	25, 1278, 1380, 1470
BseNI	ACTGG	6 cut(s)	56, 503, 880, 1331, 1363, 2144
BseRI	GAGGAG	3 cut(s)	311, 620, 1805
BseSI	GKGCMC	1 cut(s)	1277
BseXI	GCAGC	3 cut(s)	42, 2074, 2199
BseYI	CCCAGC	3 cut(s)	132, 146, 1757
Bsh1236I	CGCG	2 cut(s)	1500, 1881
BshFI	GGCC	8 cut(s)	5, 247, 882, 1132, 1238, 1491, 1979, 2022
BsiHKAI	GWGCWC	5 cut(s)	25, 1277, 1632, 1824, 1899
BsiHKCI	CYCGRG	3 cut(s)	287, 512, 776
BsiSI	CCGG	8 cut(s)	249, 488, 639, 1692, 2024, 2052, 2226, 2302
BslFI	GGGAC	2 cut(s)	1024, 2156
BslI	CCNNNNNNNGG	5 cut(s)	248, 255, 875, 1728, 2075
BsmAI	GTCTC	3 cut(s)	831, 1136, 1457
BsmFI	GGGAC	2 cut(s)	1024, 2156
BsmI	GAATGC	2 cut(s)	972, 1723
BsnI	GGCC	8 cut(s)	5, 247, 882, 1132, 1238, 1491, 1979, 2022
BsoBI	CYCGRG	3 cut(s)	287, 512, 776
Bsp1286I	GDGCHC	5 cut(s)	25, 1277, 1632, 1824, 1899
Bsp1720I	GCTNAGC	1 cut(s)	711
Bsp19I	CCATGG	1 cut(s)	832
BspACI	CCGC	8 cut(s)	457, 523, 1020, 1498, 1879, 1903, 2019, 2027
BspANI	GGCC	8 cut(s)	5, 247, 882, 1132, 1238, 1491, 1979, 2022
BspFNI	CGCG	2 cut(s)	1500, 1881
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	1 cut(s)	992
BspMI	ACCTGC	1 cut(s)	1948
BspQI	GCTCTTC	3 cut(s)	240, 482, 1620
BspTI	CTTAAG	1 cut(s)	932
BsrDI	GCAATG	4 cut(s)	25, 1278, 1380, 1470
BsrI	ACTGG	6 cut(s)	56, 503, 880, 1331, 1363, 2144
BssECI	CCNNGG	9 cut(s)	242, 637, 801, 832, 1186, 1290, 1549, 1779, 2155
BssNAI	GTATAC	1 cut(s)	2187
BssSI	CACGAG	1 cut(s)	1357
BssT1I	CCWWGG	6 cut(s)	242, 801, 832, 1290, 1779, 2155
Bst1107I	GTATAC	1 cut(s)	2187
Bst2BI	CACGAG	1 cut(s)	1357
Bst2UI	CCWGG	4 cut(s)	884, 1187, 1488, 1551
Bst4CI	ACNGT	8 cut(s)	71, 1220, 1450, 1599, 1676, 1851, 1892, 1969
Bst6I	CTCTTC	6 cut(s)	240, 482, 631, 1620, 2172, 2337
BstAFI	CTTAAG	1 cut(s)	932
BstAPI	GCANNNNNTGC	1 cut(s)	670
BstC8I	GCNNGC	4 cut(s)	497, 769, 912, 1193
BstDSI	CCRYGG	1 cut(s)	832
BstF5I	GGATG	4 cut(s)	264, 499, 580, 1162
BstFNI	CGCG	2 cut(s)	1500, 1881
BstMAI	GTCTC	3 cut(s)	831, 1136, 1457
BstMWI	GCNNNNNNNGC	4 cut(s)	670, 1497, 2019, 2052
BstNI	CCWGG	4 cut(s)	884, 1187, 1488, 1551
BstNSI	RCATGY	3 cut(s)	499, 596, 2252
BstSCI	CCNGG	8 cut(s)	247, 637, 882, 1185, 1486, 1549, 1691, 2022
BstSFI	CTRYAG	2 cut(s)	1216, 1636
BstSLI	GKGCMC	1 cut(s)	1277
BstUI	CGCG	2 cut(s)	1500, 1881
BstV1I	GCAGC	3 cut(s)	42, 2074, 2199
BstV2I	GAAGAC	2 cut(s)	287, 1304
BstX2I	RGATCY	6 cut(s)	78, 676, 795, 1110, 2094, 2148
BstYI	RGATCY	6 cut(s)	78, 676, 795, 1110, 2094, 2148
BstZ17I	GTATAC	1 cut(s)	2187
BsuRI	GGCC	8 cut(s)	5, 247, 882, 1132, 1238, 1491, 1979, 2022
BtgI	CCRYGG	1 cut(s)	832
BtsCI	GGATG	4 cut(s)	264, 499, 580, 1162
BtsIMutI	CAGTG	9 cut(s)	480, 510, 873, 1149, 1225, 1277, 1446, 1581, 2137
BveI	ACCTGC	1 cut(s)	1948
Cac8I	GCNNGC	4 cut(s)	497, 769, 912, 1193
CaiI	CAGNNNCTG	1 cut(s)	1556
CciI	TCATGA	1 cut(s)	535
Cfr13I	GGNCC	3 cut(s)	246, 745, 2021
CseI	GACGC	2 cut(s)	1016, 2261
Csp6I	GTAC	8 cut(s)	316, 448, 1306, 1324, 1348, 1416, 1579, 1816
CviQI	GTAC	8 cut(s)	316, 448, 1306, 1324, 1348, 1416, 1579, 1816
EaeI	YGGCCR	3 cut(s)	3, 880, 1130
Eam1104I	CTCTTC	6 cut(s)	240, 482, 631, 1620, 2172, 2337
EarI	CTCTTC	6 cut(s)	240, 482, 631, 1620, 2172, 2337
EciI	GGCGGA	2 cut(s)	512, 2042
Ecl136II	GAGCTC	3 cut(s)	1630, 1822, 1897
Eco130I	CCWWGG	6 cut(s)	242, 801, 832, 1290, 1779, 2155
Eco24I	GRGCYC	3 cut(s)	1632, 1824, 1899
Eco32I	GATATC	2 cut(s)	866, 961
Eco47I	GGWCC	1 cut(s)	745
Eco53kI	GAGCTC	3 cut(s)	1630, 1822, 1897
Eco57I	CTGAAG	2 cut(s)	186, 384
Eco88I	CYCGRG	3 cut(s)	287, 512, 776
EcoICRI	GAGCTC	3 cut(s)	1630, 1822, 1897
EcoRII	CCWGG	4 cut(s)	882, 1185, 1486, 1549
EcoRV	GATATC	2 cut(s)	866, 961
EcoT14I	CCWWGG	6 cut(s)	242, 801, 832, 1290, 1779, 2155
EcoT22I	ATGCAT	1 cut(s)	501
EcoT38I	GRGCYC	3 cut(s)	1632, 1824, 1899
ErhI	CCWWGG	6 cut(s)	242, 801, 832, 1290, 1779, 2155
FalI	AAGNNNNNCTT	4 cut(s)	785, 817, 1337, 1369
FaqI	GGGAC	2 cut(s)	1024, 2156
FauNDI	CATATG	1 cut(s)	649
FbaI	TGATCA	1 cut(s)	819
FblI	GTMKAC	1 cut(s)	2186
Fnu4HI	GCNGC	4 cut(s)	31, 2020, 2088, 2213
FokI	GGATG	4 cut(s)	251, 486, 587, 1169
FriOI	GRGCYC	3 cut(s)	1632, 1824, 1899
Fsp4HI	GCNGC	4 cut(s)	31, 2020, 2088, 2213
FspBI	CTAG	3 cut(s)	224, 632, 1139
GluI	GCNGC	4 cut(s)	31, 2020, 2088, 2213
GsaI	CCCAGC	3 cut(s)	136, 150, 1761
GsuI	CTGGAG	1 cut(s)	392
HaeIII	GGCC	8 cut(s)	5, 247, 882, 1132, 1238, 1491, 1979, 2022
HapII	CCGG	8 cut(s)	249, 488, 639, 1692, 2024, 2052, 2226, 2302
HgaI	GACGC	2 cut(s)	1016, 2261
HincII	GTYRAC	3 cut(s)	1602, 1741, 2104
HindII	GTYRAC	3 cut(s)	1602, 1741, 2104
HindIII	AAGCTT	2 cut(s)	654, 1724
HinfI	GANTC	7 cut(s)	59, 270, 755, 1313, 1465, 1775, 2321
HpaI	GTTAAC	1 cut(s)	1741
HpaII	CCGG	8 cut(s)	249, 488, 639, 1692, 2024, 2052, 2226, 2302
HphI	GGTGA	6 cut(s)	59, 750, 955, 1681, 1904, 2090
Hpy166II	GTNNAC	7 cut(s)	1275, 1368, 1581, 1602, 1741, 2104, 2187
Hpy188I	TCNGA	9 cut(s)	269, 1060, 1082, 1211, 1318, 1856, 1872, 2115, 2206
Hpy8I	GTNNAC	7 cut(s)	1275, 1368, 1581, 1602, 1741, 2104, 2187
Hpy99I	CGWCG	3 cut(s)	344, 1211, 2277
HpyCH4III	ACNGT	8 cut(s)	71, 1220, 1450, 1599, 1676, 1851, 1892, 1969
HpyF10VI	GCNNNNNNNGC	4 cut(s)	670, 1497, 2019, 2052
Ksp22I	TGATCA	1 cut(s)	819
KspAI	GTTAAC	1 cut(s)	1741
LguI	GCTCTTC	3 cut(s)	240, 482, 1620
LmnI	GCTCC	3 cut(s)	996, 1553, 1925
Lsp1109I	GCAGC	3 cut(s)	42, 2074, 2199
LweI	GCATC	6 cut(s)	42, 508, 543, 754, 918, 991
MaeI	CTAG	3 cut(s)	224, 632, 1139
MaeIII	GTNAC	3 cut(s)	982, 1264, 2078
MflI	RGATCY	6 cut(s)	78, 676, 795, 1110, 2094, 2148
MhlI	GDGCHC	5 cut(s)	25, 1277, 1632, 1824, 1899
MlsI	TGGCCA	2 cut(s)	882, 1132
MluCI	AATT	4 cut(s)	782, 1095, 1256, 1620
MluNI	TGGCCA	2 cut(s)	882, 1132
MlyI	GAGTC	4 cut(s)	68, 1459, 1784, 2330
MmeI	TCCRAC	4 cut(s)	27, 151, 357, 1189
Mox20I	TGGCCA	2 cut(s)	882, 1132
Mph1103I	ATGCAT	1 cut(s)	501
MscI	TGGCCA	2 cut(s)	882, 1132
MseI	TTAA	3 cut(s)	810, 933, 1740
MslI	CAYNNNNRTG	4 cut(s)	72, 534, 1385, 2285
Msp20I	TGGCCA	2 cut(s)	882, 1132
MspA1I	CMGCKG	1 cut(s)	1373
MspCI	CTTAAG	1 cut(s)	932
MspI	CCGG	8 cut(s)	249, 488, 639, 1692, 2024, 2052, 2226, 2302
MspR9I	CCNGG	8 cut(s)	249, 639, 884, 1187, 1488, 1551, 1693, 2024
Mva1269I	GAATGC	2 cut(s)	972, 1723
MvaI	CCWGG	4 cut(s)	884, 1187, 1488, 1551
MvnI	CGCG	2 cut(s)	1500, 1881
MwoI	GCNNNNNNNGC	4 cut(s)	670, 1497, 2019, 2052
NciI	CCSGG	4 cut(s)	249, 639, 1693, 2024
NcoI	CCATGG	1 cut(s)	832
NdeI	CATATG	1 cut(s)	649
NlaIV	GGNNCC	1 cut(s)	992
NmuCI	GTSAC	1 cut(s)	2078
NsiI	ATGCAT	1 cut(s)	501
NspI	RCATGY	3 cut(s)	499, 596, 2252
PaeI	GCATGC	1 cut(s)	499
PaeR7I	CTCGAG	3 cut(s)	287, 512, 776
PagI	TCATGA	1 cut(s)	535
PciI	ACATGT	1 cut(s)	592
PciSI	GCTCTTC	3 cut(s)	240, 482, 1620
PctI	GAATGC	2 cut(s)	972, 1723
PfeI	GAWTC	3 cut(s)	270, 755, 1313
PflFI	GACNNNGTC	2 cut(s)	58, 854
PflMI	CCANNNNNTGG	1 cut(s)	2075
PkrI	GCNGC	4 cut(s)	32, 2021, 2089, 2214
PleI	GAGTC	4 cut(s)	67, 1459, 1783, 2329
PpsI	GAGTC	4 cut(s)	67, 1459, 1783, 2329
PscI	ACATGT	1 cut(s)	592
Psp124BI	GAGCTC	3 cut(s)	1632, 1824, 1899
Psp6I	CCWGG	4 cut(s)	882, 1185, 1486, 1549
PspFI	CCCAGC	3 cut(s)	132, 146, 1757
PspGI	CCWGG	4 cut(s)	882, 1185, 1486, 1549
PspN4I	GGNNCC	1 cut(s)	992
PspPI	GGNCC	3 cut(s)	246, 745, 2021
PspXI	VCTCGAGB	2 cut(s)	287, 512
PstNI	CAGNNNCTG	1 cut(s)	1556
PsuI	RGATCY	6 cut(s)	78, 676, 795, 1110, 2094, 2148
PsyI	GACNNNGTC	2 cut(s)	58, 854
PvuII	CAGCTG	1 cut(s)	1373
RsaI	GTAC	8 cut(s)	317, 449, 1307, 1325, 1349, 1417, 1580, 1817
RsaNI	GTAC	8 cut(s)	316, 448, 1306, 1324, 1348, 1416, 1579, 1816
RseI	CAYNNNNRTG	4 cut(s)	72, 534, 1385, 2285
SacI	GAGCTC	3 cut(s)	1632, 1824, 1899
SapI	GCTCTTC	3 cut(s)	240, 482, 1620
SaqAI	TTAA	3 cut(s)	810, 933, 1740
SatI	GCNGC	4 cut(s)	31, 2020, 2088, 2213
Sau96I	GGNCC	3 cut(s)	246, 745, 2021
ScaI	AGTACT	1 cut(s)	317
SchI	GAGTC	4 cut(s)	68, 1459, 1784, 2330
ScrFI	CCNGG	8 cut(s)	249, 639, 884, 1187, 1488, 1551, 1693, 2024
SduI	GDGCHC	5 cut(s)	25, 1277, 1632, 1824, 1899
SfaNI	GCATC	6 cut(s)	42, 508, 543, 754, 918, 991
SfcI	CTRYAG	2 cut(s)	1216, 1636
Sfr274I	CTCGAG	3 cut(s)	287, 512, 776
SinI	GGWCC	1 cut(s)	745
SlaI	CTCGAG	3 cut(s)	287, 512, 776
SmiMI	CAYNNNNRTG	4 cut(s)	72, 534, 1385, 2285
SmlI	CTYRAG	8 cut(s)	287, 512, 776, 932, 1031, 1114, 1175, 1337
SmoI	CTYRAG	8 cut(s)	287, 512, 776, 932, 1031, 1114, 1175, 1337
SphI	GCATGC	1 cut(s)	499
Sse9I	AATT	4 cut(s)	782, 1095, 1256, 1620
SsiI	CCGC	8 cut(s)	457, 523, 1020, 1498, 1879, 1903, 2019, 2027
SspI	AATATT	1 cut(s)	1055
SspMI	CTAG	3 cut(s)	224, 632, 1139
SstI	GAGCTC	3 cut(s)	1632, 1824, 1899
StyD4I	CCNGG	8 cut(s)	247, 637, 882, 1185, 1486, 1549, 1691, 2022
StyI	CCWWGG	6 cut(s)	242, 801, 832, 1290, 1779, 2155
TaaI	ACNGT	8 cut(s)	71, 1220, 1450, 1599, 1676, 1851, 1892, 1969
TaqI	TCGA	8 cut(s)	95, 288, 342, 513, 660, 777, 1524, 2175
TaqII	GACCGA	1 cut(s)	1412
TasI	AATT	4 cut(s)	782, 1095, 1256, 1620
TatI	WGTACW	2 cut(s)	315, 1323
TauI	GCSGC	1 cut(s)	2022
TfiI	GAWTC	3 cut(s)	270, 755, 1313
Tru1I	TTAA	3 cut(s)	810, 933, 1740
Tru9I	TTAA	3 cut(s)	810, 933, 1740
TscAI	CASTG	9 cut(s)	487, 510, 880, 1156, 1225, 1277, 1453, 1588, 2144
TseFI	GTSAC	1 cut(s)	2078
TseI	GCWGC	3 cut(s)	30, 2087, 2212
Tsp45I	GTSAC	1 cut(s)	2078
TspGWI	ACGGA	2 cut(s)	1058, 1091
TspRI	CASTG	9 cut(s)	487, 510, 880, 1156, 1225, 1277, 1453, 1588, 2144
Tth111I	GACNNNGTC	2 cut(s)	58, 854
Van91I	CCANNNNNTGG	1 cut(s)	2075
Vha464I	CTTAAG	1 cut(s)	932
VneI	GTGCAC	1 cut(s)	1273
VpaK11BI	GGWCC	1 cut(s)	745
XapI	RAATTY	2 cut(s)	782, 1256
XbaI	TCTAGA	1 cut(s)	1138
XceI	RCATGY	3 cut(s)	499, 596, 2252
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1430
XhoI	CTCGAG	3 cut(s)	287, 512, 776
XmiI	GTMKAC	1 cut(s)	2186
XspI	CTAG	3 cut(s)	224, 632, 1139
ZrmI	AGTACT	1 cut(s)	317
Zsp2I	ATGCAT	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.