Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0007131

Physical Location & Seq

Genomic Coordinates Reverse (-)

4016258 .. 4020363

Gene Structure 4106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g07540.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGCTCCTCCCAAAATCTTACTCTGCGGCGACGTCTCCGGCAACCTCAAGCAGCTCTTCAAACGAGTCGTCTCGGTCAACAAATCGGCCGGTCCGTTTGACGCGTTGTTCTGCGTCGGCCAGTTCTTCCCTAACTCGGCGGACCAGCTCGACGAGTTCTCCGGTTACCTCGAAGGCCGCGCCGAGATTCCTATCCCGACCTACTTCATCGGCGACTACGGCGTCGGCAGCGCTAAGTTTCTATTGGCGGCAAGTAAGGACGCAGCGAACCAAGGCTTCAAGTTGGACGGCTTGAAGGTTTGCAACAATCTCTACTGGCTCAAAGGCAGTGGCAAATTCACCTTCCACGGGTTATCCGTGGTGTATTTGTCCGGTCTGCAGTCTTCTGTTAGTCAACAGTTTGGGACTTACAGCGAGGACGATGTCGCTGCGTTGCGAGCAATTGCTGAGGATCCCGGGATCGTTGACTTGTTCCTAACTAACGAATGGCCAAGTGGGGTTACAAATAAAGCAGCTACATCTGATGTTCCTCCCAGAGCCTCTGAGCCTTCTGGCACTGACTCTATGGTGGCAGAGTTAGTTGCTGAGATCAAACCACGCTATCATATTGCAGGCACAAAAGGAGTGTTCTATGCACGTGAGCCTTACTCCAATGTCGATGCTGTGCATGTCACCCGCTTCATAGGTCTTGCACCGGTTGGAAACAAAGATAAGCAGAAATTTCTTCATGCGCTTTCTCCAACTCCAGCATCTACTATGTCTGCAATTGAGATTCAGACAAAAACGTCAAACACTACCGTGTCTCCATACACGTATATCGAGGGAACAGCTAATGCAAAAGAAGGTGCAAAGAGGTCTAGTGATAGTGTTTCTGATTCTGATTCACAGTACTGGAGGTATGATGTCTCCCAAAAAAGGCAGAAACATGGAGATGGTAATAGGCTGTGCTTTAAGTTTGTATCCTCCGGTTCTTGTCCACGAGGGGAAACTTGCAACTTTCAACATGACATGGACGCAAGAGAACAATCTTTGAGAGGTGTTTGCTTCGAGTTTATGAACAAAGGAAAGTGTGAAAGGGGTGCTGATTGCAAATTTAAGCACAGCTTACAGGATGAAGGTGAAAGCAATTCTCACAAGAGACGCACTGGAAATTCTACCAGGTCGACAGAGTGCTGGTTTTGTTTGTCAAGCTCCAAAATAGAGTCACATCTATTTATCAGCTTAGGGGAGCATTACTACTGTGCACTTGCTAAAGGCCCACTTGTTGAAGACCACGTATTGCTAATCCCTATTGGGCATTCACCCAATACCCTTTCTCTACCCTCCGAATGTGAGATTGAGCTTGGTAAATTCCAGGATAGTCTGAAAAAGTTTTATAAGAAACAAGAAAAGGAAGTTGTTTTCTTTGAGTGGGCTTCGAAACGCTCAACTCATGCCAATCTTCAGGTCGTTCCTATTCCATCAGCCAAAGCAGCTAGTGTTCAAAATATATTTCATTTAGCTGCTGACAGACTTGGCTTCAAGTTTATGACCACAAAATTCAGTAACAGTTCTGATGGGAGAAAATCGTTGAGGACACAGTTCGATAGAAATTTTAGTTTCTTCTATGCTGAACTCCCTGATGGTACAGTCCTATCACATTTAATTGAGGAGAGCGAGAAATTCCTAGCCCAATTTGGACGCGAGGTTATGGCAGGTTTATTGAACAAGGCTGACAGGGCTGATTGGAGGAATTGTACAGATAGCAAAGAAGAGGAAACGAAGATGGTAGCAAATTTCAAGAGTAGATTTGCAGAATTTGATCCAAATAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CwfJ_C_1 CwfJ_C_2 zf-CCCH

Protein Analysis

605

Amino Acids

66.93

Weight (kDa)

7.2

Isoelectric Point (pI)

45.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCCH_DUS3L	PF25585	313 - 339	2.2e-06	DUS3L CCCH zinc finger domain
CwfJ_C_1	PF04677	381 - 498	6.6e-37	Protein similar to CwfJ C-terminal
CwfJ_C_2	PF04676	518 - 601	3.5e-19	Protein similar to CwfJ C-terminus 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007131)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56900 AT5G56900 AT5G56900
fragaria_vesca	FvH4_1g07540 FvH4_1g07540 FvH4_1g07540
malus_domestica	MD02G1080200.v1.1 MD15G1207600.v1.1
prunus_persica	Prupe.7G209200_v2.0.a1
pyrus_communis	pycom02g06370 pycom15g18400
rosa_chinensis	RchiOBHm_Chr2g0093421
rosa_laevigata	RLG00000016398
rosa_multiflora	Rmu_sc0004881.1_g000009 Rmu_sc0021911.1_g000011
rosa_roxburghii	Rroxscaffold_2G00148120
rosa_rugosa	Rorug02G0035700
rosa_samantha	Rh2AG081700 Rh2AG082300 Rh2BG083100 Rh2CG085100 Rh2DG080700 Rh2DG080800 Rh2DG081300
rosa_wichuraiana	Rw2G006490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1377
AasI	GACNNNNNNGTC	2 cut(s)	221, 784
AatII	GACGTC	1 cut(s)	36
Acc36I	ACCTGC	1 cut(s)	1685
AccI	GTMKAC	1 cut(s)	1163
AccII	CGCG	3 cut(s)	104, 180, 1683
AciI	CCGC	5 cut(s)	27, 140, 178, 248, 676
AclWI	GGATC	4 cut(s)	446, 459, 467, 1796
AcoI	YGGCCR	3 cut(s)	87, 118, 488
AcuI	CTGAAG	1 cut(s)	1427
AcvI	CACGTG	1 cut(s)	638
AcyI	GRCGYC	2 cut(s)	33, 222
AfaI	GTAC	3 cut(s)	890, 1627, 1738
AfeI	AGCGCT	1 cut(s)	232
AfiI	CCNNNNNNNGG	3 cut(s)	136, 348, 915
AflIII	ACRYGT	2 cut(s)	102, 810
AgeI	ACCGGT	1 cut(s)	694
AgsI	TTSAA	9 cut(s)	61, 280, 295, 1001, 1268, 1484, 1522, 1705, 1780
AjnI	CCWGG	2 cut(s)	1157, 1353
AleI	CACNNNNGTG	1 cut(s)	797
Alw21I	GWGCWC	1 cut(s)	1246
Alw26I	GTCTC	5 cut(s)	40, 76, 807, 910, 1132
Alw44I	GTGCAC	1 cut(s)	1242
AlwI	GGATC	4 cut(s)	446, 459, 467, 1796
Ama87I	CYCGRG	1 cut(s)	455
Aor51HI	AGCGCT	1 cut(s)	232
AoxI	GGCC	5 cut(s)	87, 118, 175, 488, 1255
ApaLI	GTGCAC	1 cut(s)	1242
ApeKI	GCWGC	7 cut(s)	52, 228, 263, 428, 512, 1472, 1502
ArsI	GACNNNNNNTTYG	2 cut(s)	54, 86
AsiGI	ACCGGT	1 cut(s)	694
AspLEI	GCGC	3 cut(s)	182, 233, 733
AspS9I	GGNCC	3 cut(s)	92, 142, 1256
AsuC2I	CCSGG	2 cut(s)	456, 457
AsuHPI	GGTGA	4 cut(s)	331, 664, 1130, 1293
AsuII	TTCGAA	1 cut(s)	1418
AvaI	CYCGRG	1 cut(s)	455
AvaII	GGWCC	2 cut(s)	92, 142
BaeGI	GKGCMC	1 cut(s)	1246
BalI	TGGCCA	1 cut(s)	490
BamHI	GGATCC	1 cut(s)	451
BauI	CACGAG	1 cut(s)	978
BbrPI	CACGTG	1 cut(s)	638
BbsI	GAAGAC	2 cut(s)	375, 1275
Bbv12I	GWGCWC	1 cut(s)	1246
BbvCI	CCTCAGC	1 cut(s)	447
BbvI	GCAGC	7 cut(s)	64, 240, 275, 415, 524, 1484, 1489
BccI	CCATC	5 cut(s)	926, 1468, 1550, 1616, 1759
BceAI	ACGGC	2 cut(s)	235, 304
BcgI	CGANNNNNNTGC	2 cut(s)	410, 444
BciT130I	CCWGG	2 cut(s)	1159, 1355
BciVI	GTATCC	1 cut(s)	970
BcnI	CCSGG	2 cut(s)	456, 457
BcoDI	GTCTC	5 cut(s)	40, 76, 807, 910, 1132
BfaI	CTAG	3 cut(s)	858, 1476, 1667
BfmI	CTRYAG	1 cut(s)	377
BfoI	RGCGCY	1 cut(s)	234
BfuAI	ACCTGC	1 cut(s)	1685
BfuI	GTATCC	1 cut(s)	970
BmcAI	AGTACT	1 cut(s)	890
Bme1390I	CCNGG	4 cut(s)	456, 457, 1159, 1355
Bme18I	GGWCC	2 cut(s)	92, 142
BmeT110I	CYCGRG	1 cut(s)	455
BmgT120I	GGNCC	3 cut(s)	92, 142, 1256
BmiI	GGNNCC	2 cut(s)	6, 453
BmrFI	CCNGG	4 cut(s)	456, 457, 1159, 1355
BmsI	GCATC	2 cut(s)	649, 758
BpiI	GAAGAC	2 cut(s)	375, 1275
BplI	GAGNNNNNCTC	2 cut(s)	632, 664
BpmI	CTGGAG	2 cut(s)	729, 913
Bpu10I	CCTNAGC	2 cut(s)	447, 1222
Bpu14I	TTCGAA	1 cut(s)	1418
BpuEI	CTTGAG	1 cut(s)	32
BpuMI	CCSGG	2 cut(s)	456, 457
BsaAI	YACGTR	3 cut(s)	638, 813, 1276
BsaBI	GATNNNNATC	1 cut(s)	191
BsaHI	GRCGYC	2 cut(s)	33, 222
BsaJI	CCNNGG	4 cut(s)	271, 346, 357, 455
BsaWI	WCCGGW	4 cut(s)	161, 371, 694, 965
BsaXI	ACNNNNNCTCC	6 cut(s)	143, 173, 787, 817, 1720, 1750
Bsc4I	CCNNNNNNNGG	3 cut(s)	136, 348, 915
Bse118I	RCCGGY	2 cut(s)	89, 694
Bse1I	ACTGG	4 cut(s)	121, 320, 896, 1150
Bse8I	GATNNNNATC	1 cut(s)	191
BseBI	CCWGG	2 cut(s)	1159, 1355
BseDI	CCNNGG	4 cut(s)	271, 346, 357, 455
BseGI	GGATG	1 cut(s)	1117
BseJI	GATNNNNATC	1 cut(s)	191
BseLI	CCNNNNNNNGG	3 cut(s)	136, 348, 915
BseMII	CTCAG	3 cut(s)	438, 534, 576
BseNI	ACTGG	4 cut(s)	121, 320, 896, 1150
BseRI	GAGGAG	1 cut(s)	1664
BseSI	GKGCMC	1 cut(s)	1246
BseX3I	CGGCCG	1 cut(s)	87
BseXI	GCAGC	7 cut(s)	64, 240, 275, 415, 524, 1484, 1489
Bsh1236I	CGCG	3 cut(s)	104, 180, 1683
Bsh1285I	CGRYCG	1 cut(s)	90
BshFI	GGCC	5 cut(s)	89, 120, 177, 490, 1257
BshTI	ACCGGT	1 cut(s)	694
BsiEI	CGRYCG	1 cut(s)	90
BsiHKAI	GWGCWC	1 cut(s)	1246
BsiHKCI	CYCGRG	1 cut(s)	455
BsiSI	CCGG	7 cut(s)	39, 90, 162, 372, 456, 695, 966
BslFI	GGGAC	1 cut(s)	418
BslI	CCNNNNNNNGG	3 cut(s)	136, 348, 915
BsmAI	GTCTC	5 cut(s)	40, 76, 807, 910, 1132
BsmBI	CGTCTC	3 cut(s)	40, 76, 1132
BsmFI	GGGAC	1 cut(s)	418
BsmI	GAATGC	1 cut(s)	1297
BsnI	GGCC	5 cut(s)	89, 120, 177, 490, 1257
BsoBI	CYCGRG	1 cut(s)	455
Bsp119I	TTCGAA	1 cut(s)	1418
Bsp1286I	GDGCHC	1 cut(s)	1246
Bsp1407I	TGTACA	1 cut(s)	1736
Bsp143I	GATC	4 cut(s)	451, 459, 588, 1801
BspACI	CCGC	5 cut(s)	27, 140, 178, 248, 676
BspANI	GGCC	5 cut(s)	89, 120, 177, 490, 1257
BspCNI	CTCAG	3 cut(s)	439, 535, 577
BspFNI	CGCG	3 cut(s)	104, 180, 1683
BspLI	GGNNCC	2 cut(s)	6, 453
BspMAI	CTGCAG	1 cut(s)	381
BspMI	ACCTGC	1 cut(s)	1685
BspPI	GGATC	4 cut(s)	446, 459, 467, 1796
BspQI	GCTCTTC	1 cut(s)	62
BspT104I	TTCGAA	1 cut(s)	1418
BsrFI	RCCGGY	2 cut(s)	89, 694
BsrGI	TGTACA	1 cut(s)	1736
BsrI	ACTGG	4 cut(s)	121, 320, 896, 1150
BssAI	RCCGGY	2 cut(s)	89, 694
BssECI	CCNNGG	4 cut(s)	271, 346, 357, 455
BssMI	GATC	4 cut(s)	451, 459, 588, 1801
BssNI	GRCGYC	2 cut(s)	33, 222
BssSI	CACGAG	1 cut(s)	978
BssT1I	CCWWGG	1 cut(s)	271
Bst2BI	CACGAG	1 cut(s)	978
Bst2UI	CCWGG	2 cut(s)	1159, 1355
Bst4CI	ACNGT	7 cut(s)	399, 799, 888, 1241, 1550, 1581, 1630
Bst6I	CTCTTC	2 cut(s)	62, 1746
BstACI	GRCGYC	2 cut(s)	33, 222
BstAUI	TGTACA	1 cut(s)	1736
BstBAI	YACGTR	3 cut(s)	638, 813, 1276
BstBI	TTCGAA	1 cut(s)	1418
BstC8I	GCNNGC	2 cut(s)	438, 613
BstDEI	CTNAG	5 cut(s)	234, 447, 543, 585, 1222
BstDSI	CCRYGG	2 cut(s)	346, 357
BstEII	GGTNACC	1 cut(s)	164
BstF5I	GGATG	1 cut(s)	1117
BstFNI	CGCG	3 cut(s)	104, 180, 1683
BstH2I	RGCGCY	1 cut(s)	234
BstHHI	GCGC	3 cut(s)	182, 233, 733
BstKTI	GATC	4 cut(s)	454, 462, 591, 1804
BstMAI	GTCTC	5 cut(s)	40, 76, 807, 910, 1132
BstMBI	GATC	4 cut(s)	451, 459, 588, 1801
BstMCI	CGRYCG	1 cut(s)	90
BstMWI	GCNNNNNNNGC	5 cut(s)	219, 228, 437, 1472, 1718
BstNI	CCWGG	2 cut(s)	1159, 1355
BstNSI	RCATGY	1 cut(s)	671
BstPI	GGTNACC	1 cut(s)	164
BstSCI	CCNGG	4 cut(s)	454, 455, 1157, 1353
BstSFI	CTRYAG	1 cut(s)	377
BstSLI	GKGCMC	1 cut(s)	1246
BstUI	CGCG	3 cut(s)	104, 180, 1683
BstV1I	GCAGC	7 cut(s)	64, 240, 275, 415, 524, 1484, 1489
BstV2I	GAAGAC	2 cut(s)	375, 1275
BstX2I	RGATCY	1 cut(s)	451
BstYI	RGATCY	1 cut(s)	451
BstZI	CGGCCG	1 cut(s)	87
BsuI	GTATCC	1 cut(s)	970
BsuRI	GGCC	5 cut(s)	89, 120, 177, 490, 1257
BtgI	CCRYGG	2 cut(s)	346, 357
BtsCI	GGATG	1 cut(s)	1117
BtsI	GCAGTG	1 cut(s)	334
BtsIMutI	CAGTG	3 cut(s)	334, 555, 1143
BveI	ACCTGC	1 cut(s)	1685
Cac8I	GCNNGC	2 cut(s)	438, 613
CfoI	GCGC	3 cut(s)	182, 233, 733
Cfr10I	RCCGGY	2 cut(s)	89, 694
Cfr13I	GGNCC	3 cut(s)	92, 142, 1256
Cfr9I	CCCGGG	1 cut(s)	455
CpoI	CGGWCCG	1 cut(s)	92
CseI	GACGC	7 cut(s)	103, 110, 211, 269, 1022, 1149, 1689
CsiI	ACCWGGT	1 cut(s)	1157
Csp6I	GTAC	3 cut(s)	889, 1626, 1737
CspAI	ACCGGT	1 cut(s)	694
CspCI	CAANNNNNGTGG	2 cut(s)	310, 345
CspI	CGGWCCG	1 cut(s)	92
CviAII	CATG	6 cut(s)	668, 728, 926, 1004, 1009, 1433
CviQI	GTAC	3 cut(s)	889, 1626, 1737
DdeI	CTNAG	5 cut(s)	234, 447, 543, 585, 1222
DpnI	GATC	4 cut(s)	453, 461, 590, 1803
DpnII	GATC	4 cut(s)	451, 459, 588, 1801
DrdI	GACNNNNNNGTC	2 cut(s)	221, 784
DseDI	GACNNNNNNGTC	2 cut(s)	221, 784
EaeI	YGGCCR	3 cut(s)	87, 118, 488
EagI	CGGCCG	1 cut(s)	87
Eam1104I	CTCTTC	2 cut(s)	62, 1746
EarI	CTCTTC	2 cut(s)	62, 1746
EciI	GGCGGA	1 cut(s)	155
EclXI	CGGCCG	1 cut(s)	87
Eco130I	CCWWGG	1 cut(s)	271
Eco47I	GGWCC	2 cut(s)	92, 142
Eco47III	AGCGCT	1 cut(s)	232
Eco52I	CGGCCG	1 cut(s)	87
Eco57I	CTGAAG	1 cut(s)	1427
Eco72I	CACGTG	1 cut(s)	638
Eco88I	CYCGRG	1 cut(s)	455
Eco91I	GGTNACC	1 cut(s)	164
EcoO65I	GGTNACC	1 cut(s)	164
EcoRII	CCWGG	2 cut(s)	1157, 1353
EcoT14I	CCWWGG	1 cut(s)	271
ErhI	CCWWGG	1 cut(s)	271
Esp3I	CGTCTC	3 cut(s)	40, 76, 1132
FaeI	CATG	6 cut(s)	671, 731, 929, 1007, 1012, 1436
FalI	AAGNNNNNCTT	6 cut(s)	41, 73, 1088, 1120, 1245, 1277
FaqI	GGGAC	1 cut(s)	418
FatI	CATG	6 cut(s)	667, 727, 925, 1003, 1008, 1432
FauI	CCCGC	1 cut(s)	683
FblI	GTMKAC	1 cut(s)	1163
FokI	GGATG	1 cut(s)	1124
FspBI	CTAG	3 cut(s)	858, 1476, 1667
GlaI	GCGC	3 cut(s)	181, 232, 732
GsuI	CTGGAG	2 cut(s)	729, 913
HaeII	RGCGCY	1 cut(s)	234
HaeIII	GGCC	5 cut(s)	89, 120, 177, 490, 1257
HapII	CCGG	7 cut(s)	39, 90, 162, 372, 456, 695, 966
HgaI	GACGC	7 cut(s)	103, 110, 211, 269, 1022, 1149, 1689
HhaI	GCGC	3 cut(s)	182, 233, 733
Hin1I	GRCGYC	2 cut(s)	33, 222
Hin1II	CATG	6 cut(s)	671, 731, 929, 1007, 1012, 1436
Hin6I	GCGC	3 cut(s)	180, 231, 731
HinP1I	GCGC	3 cut(s)	180, 231, 731
HincII	GTYRAC	4 cut(s)	79, 395, 466, 1164
HindII	GTYRAC	4 cut(s)	79, 395, 466, 1164
HinfI	GANTC	7 cut(s)	66, 187, 560, 772, 875, 881, 1202
HpaII	CCGG	7 cut(s)	39, 90, 162, 372, 456, 695, 966
HphI	GGTGA	4 cut(s)	331, 664, 1130, 1293
Hpy166II	GTNNAC	6 cut(s)	79, 395, 466, 977, 1164, 1244
Hpy188I	TCNGA	8 cut(s)	523, 544, 777, 874, 880, 1327, 1365, 1555
Hpy188III	TCNNGA	2 cut(s)	196, 1780
Hpy8I	GTNNAC	6 cut(s)	79, 395, 466, 977, 1164, 1244
Hpy99I	CGWCG	4 cut(s)	35, 119, 155, 227
HpyAV	CCTTC	6 cut(s)	167, 289, 352, 558, 836, 1109
HpyCH4III	ACNGT	7 cut(s)	399, 799, 888, 1241, 1550, 1581, 1630
HpyCH4IV	ACGT	5 cut(s)	33, 637, 785, 812, 1275
HpyF10VI	GCNNNNNNNGC	5 cut(s)	219, 228, 437, 1472, 1718
HpyF3I	CTNAG	5 cut(s)	234, 447, 543, 585, 1222
HpySE526I	ACGT	5 cut(s)	33, 637, 785, 812, 1275
Hsp92I	GRCGYC	2 cut(s)	33, 222
Hsp92II	CATG	6 cut(s)	671, 731, 929, 1007, 1012, 1436
HspAI	GCGC	3 cut(s)	180, 231, 731
Kzo9I	GATC	4 cut(s)	451, 459, 588, 1801
LguI	GCTCTTC	1 cut(s)	62
LmnI	GCTCC	3 cut(s)	10, 1196, 1228
Lsp1109I	GCAGC	7 cut(s)	64, 240, 275, 415, 524, 1484, 1489
LweI	GCATC	2 cut(s)	649, 758
MabI	ACCWGGT	1 cut(s)	1157
MaeI	CTAG	3 cut(s)	858, 1476, 1667
MaeII	ACGT	5 cut(s)	33, 637, 785, 812, 1275
MaeIII	GTNAC	5 cut(s)	164, 499, 670, 1203, 1544
MalI	GATC	4 cut(s)	453, 461, 590, 1803
MboI	GATC	4 cut(s)	451, 459, 588, 1801
MboII	GAAGA	9 cut(s)	49, 118, 375, 716, 1280, 1433, 1594, 1763, 1774
MfeI	CAATTG	2 cut(s)	441, 765
MflI	RGATCY	1 cut(s)	451
MhlI	GDGCHC	1 cut(s)	1246
MlsI	TGGCCA	1 cut(s)	490
MluI	ACGCGT	1 cut(s)	102
MluNI	TGGCCA	1 cut(s)	490
MlyI	GAGTC	3 cut(s)	75, 554, 1211
MmeI	TCCRAC	3 cut(s)	264, 679, 764
Mox20I	TGGCCA	1 cut(s)	490
MscI	TGGCCA	1 cut(s)	490
MseI	TTAA	3 cut(s)	951, 1095, 1643
MslI	CAYNNNNRTG	2 cut(s)	797, 930
Msp20I	TGGCCA	1 cut(s)	490
MspI	CCGG	7 cut(s)	39, 90, 162, 372, 456, 695, 966
MspR9I	CCNGG	4 cut(s)	456, 457, 1159, 1355
MunI	CAATTG	2 cut(s)	441, 765
Mva1269I	GAATGC	1 cut(s)	1297
MvaI	CCWGG	2 cut(s)	1159, 1355
MvnI	CGCG	3 cut(s)	104, 180, 1683
MwoI	GCNNNNNNNGC	5 cut(s)	219, 228, 437, 1472, 1718
NciI	CCSGG	2 cut(s)	456, 457
NdeII	GATC	4 cut(s)	451, 459, 588, 1801
NlaIII	CATG	6 cut(s)	671, 731, 929, 1007, 1012, 1436
NlaIV	GGNNCC	2 cut(s)	6, 453
NmeAIII	GCCGAG	2 cut(s)	116, 208
NmuCI	GTSAC	2 cut(s)	670, 1203
NspI	RCATGY	1 cut(s)	671
NspV	TTCGAA	1 cut(s)	1418
OliI	CACNNNNGTG	1 cut(s)	797
PciSI	GCTCTTC	1 cut(s)	62
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 354
PctI	GAATGC	1 cut(s)	1297
PfeI	GAWTC	4 cut(s)	187, 772, 875, 881
PflFI	GACNNNGTC	1 cut(s)	422
PfoI	TCCNGGA	1 cut(s)	1353
PinAI	ACCGGT	1 cut(s)	694
PleI	GAGTC	3 cut(s)	74, 554, 1210
PmaCI	CACGTG	1 cut(s)	638
PmlI	CACGTG	1 cut(s)	638
PpsI	GAGTC	3 cut(s)	74, 554, 1210
Ppu21I	YACGTR	3 cut(s)	638, 813, 1276
PsiI	TTATAA	1 cut(s)	1377
Psp6I	CCWGG	2 cut(s)	1157, 1353
PspCI	CACGTG	1 cut(s)	638
PspEI	GGTNACC	1 cut(s)	164
PspGI	CCWGG	2 cut(s)	1157, 1353
PspN4I	GGNNCC	2 cut(s)	6, 453
PspPI	GGNCC	3 cut(s)	92, 142, 1256
PstI	CTGCAG	1 cut(s)	381
PsuI	RGATCY	1 cut(s)	451
PsyI	GACNNNGTC	1 cut(s)	422
RsaI	GTAC	3 cut(s)	890, 1627, 1738
RsaNI	GTAC	3 cut(s)	889, 1626, 1737
RseI	CAYNNNNRTG	2 cut(s)	797, 930
Rsr2I	CGGWCCG	1 cut(s)	92
RsrII	CGGWCCG	1 cut(s)	92
SalI	GTCGAC	1 cut(s)	1162
SapI	GCTCTTC	1 cut(s)	62
SaqAI	TTAA	3 cut(s)	951, 1095, 1643
Sau3AI	GATC	4 cut(s)	451, 459, 588, 1801
Sau96I	GGNCC	3 cut(s)	92, 142, 1256
ScaI	AGTACT	1 cut(s)	890
SchI	GAGTC	3 cut(s)	75, 554, 1211
ScrFI	CCNGG	4 cut(s)	456, 457, 1159, 1355
SduI	GDGCHC	1 cut(s)	1246
SexAI	ACCWGGT	1 cut(s)	1157
SfaNI	GCATC	2 cut(s)	649, 758
SfcI	CTRYAG	1 cut(s)	377
SfuI	TTCGAA	1 cut(s)	1418
SinI	GGWCC	2 cut(s)	92, 142
SmaI	CCCGGG	1 cut(s)	457
SmiMI	CAYNNNNRTG	2 cut(s)	797, 930
SmlI	CTYRAG	1 cut(s)	47
SmoI	CTYRAG	1 cut(s)	47
SsiI	CCGC	5 cut(s)	27, 140, 178, 248, 676
SspMI	CTAG	3 cut(s)	858, 1476, 1667
StyD4I	CCNGG	4 cut(s)	454, 455, 1157, 1353
StyI	CCWWGG	1 cut(s)	271
TaaI	ACNGT	7 cut(s)	399, 799, 888, 1241, 1550, 1581, 1630
TaiI	ACGT	5 cut(s)	36, 640, 788, 815, 1278
TaqI	TCGA	8 cut(s)	150, 171, 657, 819, 1047, 1163, 1418, 1584
TaqII	GACCGA	1 cut(s)	64
TatI	WGTACW	2 cut(s)	888, 1736
TauI	GCSGC	3 cut(s)	30, 180, 251
TfiI	GAWTC	4 cut(s)	187, 772, 875, 881
Tru1I	TTAA	3 cut(s)	951, 1095, 1643
Tru9I	TTAA	3 cut(s)	951, 1095, 1643
TscAI	CASTG	3 cut(s)	334, 562, 1150
TseFI	GTSAC	2 cut(s)	670, 1203
TseI	GCWGC	7 cut(s)	52, 228, 263, 428, 512, 1472, 1502
Tsp45I	GTSAC	2 cut(s)	670, 1203
TspDTI	ATGAA	6 cut(s)	196, 670, 716, 1070, 1128, 1484
TspGWI	ACGGA	2 cut(s)	84, 346
TspMI	CCCGGG	1 cut(s)	455
TspRI	CASTG	3 cut(s)	334, 562, 1150
Tth111I	GACNNNGTC	1 cut(s)	422
VneI	GTGCAC	1 cut(s)	1242
VpaK11BI	GGWCC	2 cut(s)	92, 142
XceI	RCATGY	1 cut(s)	671
XmaI	CCCGGG	1 cut(s)	455
XmiI	GTMKAC	1 cut(s)	1163
XspI	CTAG	3 cut(s)	858, 1476, 1667
ZraI	GACGTC	1 cut(s)	34
ZrmI	AGTACT	1 cut(s)	890

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.