Rh2AG082300

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007131
Physical Location & Seq
Reverse (-)
6980890 .. 6984866
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG082300.1

Sequence Viewer

Length: 1539 bp
ATGGACGGCTTGAAGGTTTGCAGCAATCTCTACTGGCTGAAAGGCAGTGGCAAATTCACCTTCCACGGGTTATCGGTGGTGTATTTGTCTGGTCTGCAATCTTCTGATAGTCAACAGTTTGGGACTTACAGTCAGGATGATGTTGATGCATTGCGAGCAATTGCTGAAGATCCTGGGATCGTTGACTTGTTCCTAACTAACAAATGGCCAAGTGGGATTACAAATAAAGCAGGTACATCTGATGTTCCTCCTGGAGCCTCTGAGCCTTTTGGCACTGACTCTACTGTGGCAGAGTTAGCAGCCGAGATCAAACCACGCTATCATGTTGCAGGCACAAAAGGCATGTTCTATGCACGTGAGCCTTACTCCAATGTCGATGCTGTGCATGTCACCCGCTTCATAGGTCTTGCTCCAGTTGGAAACAAAGACAAGCAGAAATTTCTTCATGCGCTTTCTCCTACTCCAGCATCTACTATGTCTGCTCTTGAAATTAAGACAAAATCGCCAAACACTACCTTGTCTCCATACATGTATATCGAGGGAACAGCTAATGCGAAAGAAGGTGCAAAGAGGTCTAGTGATAGTGTTTCTGATTCTGATTCACAGTACTGGAGATATGATGTCTCCCAAAAAAGGCAGAAACATGGAGCTGGAGATGGTAATAGGCTGTGCTTTAAGTTTGTATCCTCCGGTTCTTGTCCACGAGGGGAAACTTGCAACTTTCAACATGACATGGATGCAAGAGAACAATCTTTGAGAGGTGTTTGCTTCGATTTTATGAACAAAGGAAAGTGTGAAAGGGGTACTGATTGCAAATTTAAGCACAGCTTACAGGATGAAGGTGAAAGCAATTCTCACAAAAGACGCACTGGAAATGCTACCAGGTCGACAGAGTGCTGGTTTTGTTTGTCAAGCTCCAAAATAGAGTCTCATCTATTTATCAGCTTAGGGGAGCATTACTACTGTGCACTTGCTAAAGGCCCACTTGTTGAAGACCACGTATTGCTAATCCCTATTGGGCATTCACCCAATACCCTTTCTCTACCCTCCGAATGTGAGATGGAGCTTGGTAAATTCCAGGATAGTCTTAAAAAGTTTTATAAGAAACAAGACAAGGAAGTAGTTTTCTTTGAGTGGGCTTCAAAACGTTCAACTCATGCCAATCTTCAGGTCGTTCCTATTCCGTCGGCCAAAGCAGCTAGTGTTCAAAATTTATTTCATTTAGCTGCTGACAGGTTCCAATTCAAATTTACGACCACAAAATTCAGTAACAGTTCTGATGGGAGAAAATCGTTGAGGACCCAGTTCGATAGAAATTTTAGCTTCTTCTATGCTGAGCTCCCTGATGGTACAGTCCTATCACATTTAATTGAGGAGAGCGAGAATTTCCTACCCCAATTTGGACGCGAGGTTATGGCAGGTTTATTGAACAAGGCTAACAGGGCTGATTGGAGGAGGTGTACAGATAGCAAAGAAGAGGAAACGAAGATGGTAGAAAAATTCAAGAGTAGATTCGAAGAATTTGATCCAAATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CwfJ_C_1 CwfJ_C_2 zf-CCCH

Protein Analysis

512

Amino Acids

57.31

Weight (kDa)

7.57

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_DUS3L PF25585 221 - 247 1.8e-06 DUS3L CCCH zinc finger domain
CwfJ_C_1 PF04677 289 - 406 2e-37 Protein similar to CwfJ C-terminal
CwfJ_C_2 PF04676 423 - 509 5.4e-20 Protein similar to CwfJ C-terminus 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1101
Acc36I ACCTGC 2 cut(s) 221, 1409
AccI GTMKAC 1 cut(s) 887
AccII CGCG 1 cut(s) 1407
AciI CCGC 1 cut(s) 394
AclI AACGTT 1 cut(s) 1147
AclWI GGATC 3 cut(s) 164, 185, 1520
AcoI YGGCCR 2 cut(s) 206, 1188
AcuI CTGAAG 2 cut(s) 186, 1151
AcvI CACGTG 1 cut(s) 356
AfaI GTAC 5 cut(s) 235, 608, 805, 1351, 1462
AfiI CCNNNNNNNGG 3 cut(s) 66, 633, 1400
AflIII ACRYGT 1 cut(s) 528
AhdI GACNNNNNGTC 1 cut(s) 129
AjnI CCWGG 4 cut(s) 172, 250, 881, 1077
Alw21I GWGCWC 2 cut(s) 970, 1341
Alw26I GTCTC 3 cut(s) 525, 628, 933
Alw44I GTGCAC 1 cut(s) 966
AlwI GGATC 3 cut(s) 164, 185, 1520
AoxI GGCC 3 cut(s) 206, 979, 1188
ApaLI GTGCAC 1 cut(s) 966
ApeKI GCWGC 4 cut(s) 21, 299, 1196, 1226
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 2 cut(s) 980, 1299
AsuHPI GGTGA 4 cut(s) 49, 382, 854, 1017
AsuII TTCGAA 1 cut(s) 1515
AvaII GGWCC 1 cut(s) 1299
BaeGI GKGCMC 1 cut(s) 970
BalI TGGCCA 1 cut(s) 208
BanII GRGCYC 1 cut(s) 1341
BauI CACGAG 1 cut(s) 702
BbrPI CACGTG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 999
Bbv12I GWGCWC 2 cut(s) 970, 1341
BbvI GCAGC 4 cut(s) 33, 311, 1208, 1213
BccI CCATC 5 cut(s) 650, 1054, 1274, 1340, 1483
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 4 cut(s) 174, 252, 883, 1079
BciVI GTATCC 1 cut(s) 694
BcoDI GTCTC 3 cut(s) 525, 628, 933
BfaI CTAG 2 cut(s) 576, 1200
BfuAI ACCTGC 2 cut(s) 221, 1409
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 4 cut(s) 22, 300, 1197, 1227
BlpI GCTNAGC 1 cut(s) 1335
BlsI GCNGC 4 cut(s) 23, 301, 1198, 1228
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 4 cut(s) 174, 252, 883, 1079
Bme18I GGWCC 1 cut(s) 1299
BmeRI GACNNNNNGTC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 980, 1299
BmiI GGNNCC 3 cut(s) 256, 1238, 1301
BmrFI CCNGG 4 cut(s) 174, 252, 883, 1079
BmrI ACTGGG 1 cut(s) 1297
BmsI GCATC 4 cut(s) 136, 367, 476, 727
BmuI ACTGGG 1 cut(s) 1297
BpiI GAAGAC 1 cut(s) 999
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpmI CTGGAG 5 cut(s) 273, 396, 447, 631, 672
Bpu10I CCTNAGC 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 1335
Bpu14I TTCGAA 1 cut(s) 1515
BsaAI YACGTR 2 cut(s) 356, 1000
BsaJI CCNNGG 2 cut(s) 64, 173
BsaWI WCCGGW 1 cut(s) 689
BsaXI ACNNNNNCTCC 4 cut(s) 505, 535, 1444, 1474
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 633, 1400
Bse1I ACTGG 5 cut(s) 38, 413, 614, 874, 1303
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 4 cut(s) 174, 252, 883, 1079
BseDI CCNNGG 2 cut(s) 64, 173
BseGI GGATG 3 cut(s) 142, 742, 841
BseLI CCNNNNNNNGG 3 cut(s) 66, 633, 1400
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 252, 1326
BseNI ACTGG 5 cut(s) 38, 413, 614, 874, 1303
BseRI GAGGAG 2 cut(s) 1388, 1468
BseSI GKGCMC 1 cut(s) 970
BseXI GCAGC 4 cut(s) 33, 311, 1208, 1213
Bsh1236I CGCG 1 cut(s) 1407
BshFI GGCC 3 cut(s) 208, 981, 1190
BsiHKAI GWGCWC 2 cut(s) 970, 1341
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 3 cut(s) 66, 633, 1400
BsmAI GTCTC 3 cut(s) 525, 628, 933
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 1 cut(s) 1021
BsnI GGCC 3 cut(s) 208, 981, 1190
Bsp119I TTCGAA 1 cut(s) 1515
Bsp1286I GDGCHC 2 cut(s) 970, 1341
Bsp1407I TGTACA 1 cut(s) 1460
Bsp143I GATC 4 cut(s) 169, 177, 306, 1525
Bsp1720I GCTNAGC 1 cut(s) 1335
BspACI CCGC 1 cut(s) 394
BspANI GGCC 3 cut(s) 208, 981, 1190
BspCNI CTCAG 2 cut(s) 253, 1327
BspFNI CGCG 1 cut(s) 1407
BspLI GGNNCC 3 cut(s) 256, 1238, 1301
BspMI ACCTGC 2 cut(s) 221, 1409
BspPI GGATC 3 cut(s) 164, 185, 1520
BspT104I TTCGAA 1 cut(s) 1515
BsrDI GCAATG 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 1460
BsrI ACTGG 5 cut(s) 38, 413, 614, 874, 1303
BssECI CCNNGG 2 cut(s) 64, 173
BssMI GATC 4 cut(s) 169, 177, 306, 1525
BssSI CACGAG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 4 cut(s) 174, 252, 883, 1079
Bst4CI ACNGT 7 cut(s) 117, 131, 286, 606, 965, 1274, 1354
Bst6I CTCTTC 1 cut(s) 1470
BstAUI TGTACA 1 cut(s) 1460
BstBAI YACGTR 2 cut(s) 356, 1000
BstBI TTCGAA 1 cut(s) 1515
BstC8I GCNNGC 2 cut(s) 156, 331
BstDEI CTNAG 3 cut(s) 261, 946, 1335
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 142, 742, 841
BstFNI CGCG 1 cut(s) 1407
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 4 cut(s) 172, 180, 309, 1528
BstMAI GTCTC 3 cut(s) 525, 628, 933
BstMBI GATC 4 cut(s) 169, 177, 306, 1525
BstMWI GCNNNNNNNGC 5 cut(s) 155, 296, 339, 1196, 1442
BstNI CCWGG 4 cut(s) 174, 252, 883, 1079
BstNSI RCATGY 3 cut(s) 346, 389, 532
BstSCI CCNGG 4 cut(s) 172, 250, 881, 1077
BstSLI GKGCMC 1 cut(s) 970
BstUI CGCG 1 cut(s) 1407
BstV1I GCAGC 4 cut(s) 33, 311, 1208, 1213
BstV2I GAAGAC 1 cut(s) 999
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 3 cut(s) 208, 981, 1190
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 142, 742, 841
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 3 cut(s) 52, 273, 867
BveI ACCTGC 2 cut(s) 221, 1409
Cac8I GCNNGC 2 cut(s) 156, 331
CfoI GCGC 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 980, 1299
CseI GACGC 2 cut(s) 873, 1413
CsiI ACCWGGT 1 cut(s) 881
Csp6I GTAC 5 cut(s) 234, 607, 804, 1350, 1461
CviAII CATG 9 cut(s) 323, 343, 386, 446, 529, 644, 728, 733, 1157
CviQI GTAC 5 cut(s) 234, 607, 804, 1350, 1461
DdeI CTNAG 3 cut(s) 261, 946, 1335
DpnI GATC 4 cut(s) 171, 179, 308, 1527
DpnII GATC 4 cut(s) 169, 177, 306, 1525
DriI GACNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 206, 1188
Eam1104I CTCTTC 1 cut(s) 1470
Eam1105I GACNNNNNGTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 1470
Ecl136II GAGCTC 1 cut(s) 1339
Eco24I GRGCYC 1 cut(s) 1341
Eco47I GGWCC 1 cut(s) 1299
Eco53kI GAGCTC 1 cut(s) 1339
Eco57I CTGAAG 2 cut(s) 186, 1151
Eco72I CACGTG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 1339
EcoO109I RGGNCCY 1 cut(s) 1299
EcoRII CCWGG 4 cut(s) 172, 250, 881, 1077
EcoT22I ATGCAT 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 1341
FaeI CATG 9 cut(s) 326, 346, 389, 449, 532, 647, 731, 736, 1160
FalI AAGNNNNNCTT 4 cut(s) 812, 844, 969, 1001
FaqI GGGAC 1 cut(s) 136
FatI CATG 9 cut(s) 322, 342, 385, 445, 528, 643, 727, 732, 1156
FauI CCCGC 1 cut(s) 401
FblI GTMKAC 1 cut(s) 887
Fnu4HI GCNGC 4 cut(s) 22, 300, 1197, 1227
FokI GGATG 3 cut(s) 149, 749, 848
FriOI GRGCYC 1 cut(s) 1341
Fsp4HI GCNGC 4 cut(s) 22, 300, 1197, 1227
FspBI CTAG 2 cut(s) 576, 1200
GlaI GCGC 1 cut(s) 450
GluI GCNGC 4 cut(s) 22, 300, 1197, 1227
GsuI CTGGAG 5 cut(s) 273, 396, 447, 631, 672
HaeIII GGCC 3 cut(s) 208, 981, 1190
HapII CCGG 1 cut(s) 690
HgaI GACGC 2 cut(s) 873, 1413
HhaI GCGC 1 cut(s) 451
Hin1II CATG 9 cut(s) 326, 346, 389, 449, 532, 647, 731, 736, 1160
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 3 cut(s) 113, 184, 888
HindII GTYRAC 3 cut(s) 113, 184, 888
HinfI GANTC 5 cut(s) 278, 593, 599, 926, 1512
HpaII CCGG 1 cut(s) 690
HphI GGTGA 4 cut(s) 49, 382, 854, 1017
Hpy166II GTNNAC 6 cut(s) 113, 184, 701, 888, 968, 1461
Hpy188I TCNGA 7 cut(s) 106, 241, 262, 592, 598, 1051, 1279
Hpy188III TCNNGA 3 cut(s) 134, 485, 1504
Hpy8I GTNNAC 6 cut(s) 113, 184, 701, 888, 968, 1461
Hpy99I CGWCG 1 cut(s) 1189
HpyAV CCTTC 4 cut(s) 7, 70, 554, 833
HpyCH4III ACNGT 7 cut(s) 117, 131, 286, 606, 965, 1274, 1354
HpyCH4IV ACGT 3 cut(s) 355, 999, 1147
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 296, 339, 1196, 1442
HpyF3I CTNAG 3 cut(s) 261, 946, 1335
HpySE526I ACGT 3 cut(s) 355, 999, 1147
Hsp92II CATG 9 cut(s) 326, 346, 389, 449, 532, 647, 731, 736, 1160
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 4 cut(s) 169, 177, 306, 1525
LmnI GCTCC 7 cut(s) 254, 415, 647, 920, 952, 1063, 1344
Lsp1109I GCAGC 4 cut(s) 33, 311, 1208, 1213
LweI GCATC 4 cut(s) 136, 367, 476, 727
MabI ACCWGGT 1 cut(s) 881
MaeI CTAG 2 cut(s) 576, 1200
MaeII ACGT 3 cut(s) 355, 999, 1147
MaeIII GTNAC 2 cut(s) 388, 1268
MalI GATC 4 cut(s) 171, 179, 308, 1527
MboI GATC 4 cut(s) 169, 177, 306, 1525
MboII GAAGA 9 cut(s) 93, 179, 434, 1004, 1157, 1318, 1487, 1498, 1529
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 2 cut(s) 970, 1341
MlsI TGGCCA 1 cut(s) 208
MluNI TGGCCA 1 cut(s) 208
MlyI GAGTC 2 cut(s) 272, 935
MmeI TCCRAC 1 cut(s) 397
Mox20I TGGCCA 1 cut(s) 208
Mph1103I ATGCAT 1 cut(s) 151
MscI TGGCCA 1 cut(s) 208
MseI TTAA 5 cut(s) 492, 675, 819, 1089, 1367
Msp20I TGGCCA 1 cut(s) 208
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 4 cut(s) 174, 252, 883, 1079
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 4 cut(s) 174, 252, 883, 1079
MvnI CGCG 1 cut(s) 1407
MwoI GCNNNNNNNGC 5 cut(s) 155, 296, 339, 1196, 1442
NdeII GATC 4 cut(s) 169, 177, 306, 1525
NlaIII CATG 9 cut(s) 326, 346, 389, 449, 532, 647, 731, 736, 1160
NlaIV GGNNCC 3 cut(s) 256, 1238, 1301
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 3 cut(s) 346, 389, 532
NspV TTCGAA 1 cut(s) 1515
PciI ACATGT 1 cut(s) 528
PctI GAATGC 1 cut(s) 1021
PfeI GAWTC 3 cut(s) 593, 599, 1512
PfoI TCCNGGA 2 cut(s) 250, 1077
PkrI GCNGC 4 cut(s) 23, 301, 1198, 1228
PleI GAGTC 2 cut(s) 272, 934
PmaCI CACGTG 1 cut(s) 356
PmlI CACGTG 1 cut(s) 356
PpsI GAGTC 2 cut(s) 272, 934
Ppu21I YACGTR 2 cut(s) 356, 1000
PpuMI RGGWCCY 1 cut(s) 1299
PscI ACATGT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 1341
Psp1406I AACGTT 1 cut(s) 1147
Psp5II RGGWCCY 1 cut(s) 1299
Psp6I CCWGG 4 cut(s) 172, 250, 881, 1077
PspCI CACGTG 1 cut(s) 356
PspGI CCWGG 4 cut(s) 172, 250, 881, 1077
PspN4I GGNNCC 3 cut(s) 256, 1238, 1301
PspPI GGNCC 2 cut(s) 980, 1299
PspPPI RGGWCCY 1 cut(s) 1299
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 5 cut(s) 235, 608, 805, 1351, 1462
RsaNI GTAC 5 cut(s) 234, 607, 804, 1350, 1461
SacI GAGCTC 1 cut(s) 1341
SalI GTCGAC 1 cut(s) 886
SaqAI TTAA 5 cut(s) 492, 675, 819, 1089, 1367
SatI GCNGC 4 cut(s) 22, 300, 1197, 1227
Sau3AI GATC 4 cut(s) 169, 177, 306, 1525
Sau96I GGNCC 2 cut(s) 980, 1299
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 2 cut(s) 272, 935
ScrFI CCNGG 4 cut(s) 174, 252, 883, 1079
SduI GDGCHC 2 cut(s) 970, 1341
SexAI ACCWGGT 1 cut(s) 881
SfaNI GCATC 4 cut(s) 136, 367, 476, 727
SfuI TTCGAA 1 cut(s) 1515
SinI GGWCC 1 cut(s) 1299
SsiI CCGC 1 cut(s) 394
SspMI CTAG 2 cut(s) 576, 1200
SstI GAGCTC 1 cut(s) 1341
StyD4I CCNGG 4 cut(s) 172, 250, 881, 1077
TaaI ACNGT 7 cut(s) 117, 131, 286, 606, 965, 1274, 1354
TaiI ACGT 3 cut(s) 358, 1002, 1150
TaqI TCGA 6 cut(s) 375, 537, 771, 887, 1308, 1515
TatI WGTACW 2 cut(s) 606, 1460
TfiI GAWTC 3 cut(s) 593, 599, 1512
Tru1I TTAA 5 cut(s) 492, 675, 819, 1089, 1367
Tru9I TTAA 5 cut(s) 492, 675, 819, 1089, 1367
TscAI CASTG 3 cut(s) 52, 280, 874
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 4 cut(s) 21, 299, 1196, 1226
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 5 cut(s) 388, 434, 794, 852, 1208
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 3 cut(s) 52, 280, 874
VneI GTGCAC 1 cut(s) 966
VpaK11BI GGWCC 1 cut(s) 1299
XceI RCATGY 3 cut(s) 346, 389, 532
XmiI GTMKAC 1 cut(s) 887
XspI CTAG 2 cut(s) 576, 1200
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.