FvH4_1g10140

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010997
Physical Location & Seq
Reverse (-)
5507718 .. 5509252
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10140.t1

Sequence Viewer

Length: 627 bp
ATGACCCATATGCCTCGCTCACAGAGATTCCTCACCACAGCCAACTACACCGCCGCAGCGCCGCCTCCCGAAGCCATGGCCTTGGAGTCCGACTTCGTCGTCATCCTGGCAGCTCTCTTATGCGCCGTCATATGCGTCGTGGGGTTATTAGCCGTGGCCCGCTGCGCGTGGCTGCGACGCGCCCCGGTTCCCGGCGGCCAGTCCCAGGCCTCTTCAGCTGCCAACAAGGGCCTGAAGAAGAAGGTGCTCCAGCAGCTACCCAAGTTCACCTACGGCGACGGAGGACCGGCGGCGCCGCCGAAGATGGGGTCGGAGTGCGCCATATGCCTCGGCGAGTTCGCGGAGGGCGACGAGATTAGAGCTCTGCCGCAGTGCGGCCATGTCTTCCACGTCGGATGCATCGACACGTGGCTCGGCTCGCACTCGTCGTGTCCGTCGTGCCGGCAGGTCCTGGTGGTGGCGCGTTGTCACAAGTGCGGCAACTTCCCCGCCATTCCTGAATCTGAGCCTAAAACTCGGCTGGATCATATTTACCATTCTGCCCCTCCACCACCACCCTCCACCGCCTCAACTACCACTTGTAGCAACAGCTTCAGTAACAGTAATAGTTTTGTTTTCTTACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

209

Amino Acids

22.03

Weight (kDa)

8.47

Isoelectric Point (pI)

65.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 105 - 148 8e-16 Ring finger domain
zf-RING_11 PF17123 105 - 134 4.4e-12 RING-like zinc finger
zf-C3HC4_2 PF13923 105 - 147 3.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 105 - 149 2.4e-06 zinc-RING finger domain
zf-C3HC4 PF00097 106 - 147 4.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 106 - 145 1.8e-06 RING-type zinc-finger
zf-rbx1 PF12678 116 - 148 6.8e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 292
AccII CGCG 4 cut(s) 167, 180, 341, 463
AclWI GGATC 1 cut(s) 531
AcoI YGGCCR 2 cut(s) 196, 376
AcuI CTGAAG 3 cut(s) 198, 254, 577
AcvI CACGTG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 293
AfiI CCNNNNNNNGG 5 cut(s) 191, 205, 305, 374, 457
AflIII ACRYGT 1 cut(s) 405
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 3 cut(s) 105, 204, 450
AluBI AGCT 5 cut(s) 113, 218, 256, 362, 591
AluI AGCT 5 cut(s) 113, 218, 256, 362, 591
Alw21I GWGCWC 2 cut(s) 249, 364
AlwI GGATC 1 cut(s) 531
AlwNI CAGNNNCTG 1 cut(s) 451
AoxI GGCC 6 cut(s) 78, 156, 196, 207, 229, 376
ApeKI GCWGC 6 cut(s) 56, 110, 162, 172, 218, 253
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspLEI GCGC 7 cut(s) 61, 125, 167, 182, 295, 320, 463
AspS9I GGNCC 4 cut(s) 157, 229, 284, 448
AsuC2I CCSGG 2 cut(s) 185, 192
AsuHPI GGTGA 2 cut(s) 25, 259
AvaII GGWCC 2 cut(s) 284, 448
BanI GGYRCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 364
BbrPI CACGTG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 249, 364
BbvI GCAGC 6 cut(s) 68, 122, 149, 159, 205, 265
BccI CCATC 1 cut(s) 298
BceAI ACGGC 3 cut(s) 110, 137, 289
BciT130I CCWGG 3 cut(s) 107, 206, 452
BcnI CCSGG 2 cut(s) 185, 192
BfoI RGCGCY 2 cut(s) 62, 296
BfuAI ACCTGC 1 cut(s) 436
Bme1390I CCNGG 5 cut(s) 107, 185, 192, 206, 452
Bme18I GGWCC 2 cut(s) 284, 448
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 4 cut(s) 157, 229, 284, 448
BmiI GGNNCC 2 cut(s) 189, 294
BmrFI CCNGG 5 cut(s) 107, 185, 192, 206, 452
BmsI GCATC 2 cut(s) 386, 408
BpiI GAAGAC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 233
BpuMI CCSGG 2 cut(s) 185, 192
BsaAI YACGTR 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 6 cut(s) 75, 81, 153, 183, 204, 328
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 205, 305, 374, 457
Bse118I RCCGGY 2 cut(s) 286, 441
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 3 cut(s) 107, 206, 452
BseDI CCNNGG 6 cut(s) 75, 81, 153, 183, 204, 328
BseGI GGATG 2 cut(s) 102, 401
BseLI CCNNNNNNNGG 5 cut(s) 191, 205, 305, 374, 457
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 1 cut(s) 199
BseXI GCAGC 6 cut(s) 68, 122, 149, 159, 205, 265
Bsh1236I CGCG 4 cut(s) 167, 180, 341, 463
BshFI GGCC 6 cut(s) 80, 158, 198, 209, 231, 378
BshNI GGYRCC 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 249, 364
BsiSI CCGG 4 cut(s) 185, 192, 287, 442
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 5 cut(s) 191, 205, 305, 374, 457
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 6 cut(s) 80, 158, 198, 209, 231, 378
Bsp1286I GDGCHC 2 cut(s) 249, 364
Bsp143I GATC 1 cut(s) 523
Bsp19I CCATGG 1 cut(s) 75
BspANI GGCC 6 cut(s) 80, 158, 198, 209, 231, 378
BspCNI CTCAG 1 cut(s) 496
BspFNI CGCG 4 cut(s) 167, 180, 341, 463
BspLI GGNNCC 2 cut(s) 189, 294
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 292
BsrFI RCCGGY 2 cut(s) 286, 441
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 2 cut(s) 286, 441
BssECI CCNNGG 6 cut(s) 75, 81, 153, 183, 204, 328
BssMI GATC 1 cut(s) 523
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 75, 81
Bst2UI CCWGG 3 cut(s) 107, 206, 452
Bst4CI ACNGT 1 cut(s) 602
Bst6I CTCTTC 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 160, 419, 443
BstDEI CTNAG 1 cut(s) 504
BstDSI CCRYGG 2 cut(s) 75, 153
BstF5I GGATG 2 cut(s) 102, 401
BstFNI CGCG 4 cut(s) 167, 180, 341, 463
BstH2I RGCGCY 2 cut(s) 62, 296
BstHHI GCGC 7 cut(s) 61, 125, 167, 182, 295, 320, 463
BstKTI GATC 1 cut(s) 526
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 5 cut(s) 164, 215, 253, 324, 418
BstNI CCWGG 3 cut(s) 107, 206, 452
BstSCI CCNGG 5 cut(s) 105, 183, 190, 204, 450
BstUI CGCG 4 cut(s) 167, 180, 341, 463
BstV1I GCAGC 6 cut(s) 68, 122, 149, 159, 205, 265
BstV2I GAAGAC 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 82
BsuRI GGCC 6 cut(s) 80, 158, 198, 209, 231, 378
BtgI CCRYGG 2 cut(s) 75, 153
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 2 cut(s) 102, 401
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 1 cut(s) 377
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 160, 419, 443
CaiI CAGNNNCTG 1 cut(s) 451
CfoI GCGC 7 cut(s) 61, 125, 167, 182, 295, 320, 463
Cfr10I RCCGGY 2 cut(s) 286, 441
Cfr13I GGNCC 4 cut(s) 157, 229, 284, 448
CseI GACGC 2 cut(s) 124, 186
CviAII CATG 2 cut(s) 76, 380
DdeI CTNAG 1 cut(s) 504
DinI GGCGCC 1 cut(s) 294
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 2 cut(s) 196, 376
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 75, 81
Eco147I AGGCCT 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 284, 448
Eco53kI GAGCTC 1 cut(s) 362
Eco57I CTGAAG 3 cut(s) 198, 254, 577
Eco72I CACGTG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 362
EcoO109I RGGNCCY 2 cut(s) 229, 448
EcoRII CCWGG 3 cut(s) 105, 204, 450
EcoT14I CCWWGG 2 cut(s) 75, 81
EcoT22I ATGCAT 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 364
EgeI GGCGCC 1 cut(s) 294
EheI GGCGCC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 75, 81
FaeI CATG 2 cut(s) 79, 383
FaqI GGGAC 1 cut(s) 187
FatI CATG 2 cut(s) 75, 379
FauI CCCGC 2 cut(s) 167, 496
FauNDI CATATG 3 cut(s) 9, 131, 323
FokI GGATG 2 cut(s) 89, 408
FriOI GRGCYC 1 cut(s) 364
GlaI GCGC 7 cut(s) 60, 124, 166, 181, 294, 319, 462
GsuI CTGGAG 1 cut(s) 233
HaeII RGCGCY 2 cut(s) 62, 296
HaeIII GGCC 6 cut(s) 80, 158, 198, 209, 231, 378
HapII CCGG 4 cut(s) 185, 192, 287, 442
HgaI GACGC 2 cut(s) 124, 186
HhaI GCGC 7 cut(s) 61, 125, 167, 182, 295, 320, 463
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 2 cut(s) 79, 383
Hin6I GCGC 7 cut(s) 59, 123, 165, 180, 293, 318, 461
HinP1I GCGC 7 cut(s) 59, 123, 165, 180, 293, 318, 461
HinfI GANTC 3 cut(s) 27, 86, 500
HpaII CCGG 4 cut(s) 185, 192, 287, 442
HphI GGTGA 2 cut(s) 25, 259
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 4 cut(s) 91, 313, 395, 505
Hpy188III TCNNGA 2 cut(s) 68, 497
Hpy8I GTNNAC 1 cut(s) 267
Hpy99I CGWCG 8 cut(s) 101, 140, 180, 281, 353, 395, 430, 439
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 602
HpyCH4IV ACGT 2 cut(s) 390, 407
HpyCH4V TGCA 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 215, 253, 324, 418
HpyF3I CTNAG 1 cut(s) 504
HpySE526I ACGT 2 cut(s) 390, 407
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 2 cut(s) 79, 383
HspAI GCGC 7 cut(s) 59, 123, 165, 180, 293, 318, 461
KasI GGCGCC 1 cut(s) 292
KroI GCCGGC 1 cut(s) 441
KroNI GCCGGC 1 cut(s) 443
Kzo9I GATC 1 cut(s) 523
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 6 cut(s) 68, 122, 149, 159, 205, 265
LweI GCATC 2 cut(s) 386, 408
MaeII ACGT 2 cut(s) 390, 407
MaeIII GTNAC 2 cut(s) 467, 596
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 5 cut(s) 204, 247, 250, 313, 376
MhlI GDGCHC 2 cut(s) 249, 364
Mly113I GGCGCC 1 cut(s) 293
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 114, 291, 373
Mph1103I ATGCAT 1 cut(s) 401
MroNI GCCGGC 1 cut(s) 441
MspA1I CMGCKG 2 cut(s) 162, 218
MspI CCGG 4 cut(s) 185, 192, 287, 442
MspR9I CCNGG 5 cut(s) 107, 185, 192, 206, 452
MvaI CCWGG 3 cut(s) 107, 206, 452
MvnI CGCG 4 cut(s) 167, 180, 341, 463
MwoI GCNNNNNNNGC 5 cut(s) 164, 215, 253, 324, 418
NaeI GCCGGC 1 cut(s) 443
NarI GGCGCC 1 cut(s) 293
NciI CCSGG 2 cut(s) 185, 192
NcoI CCATGG 1 cut(s) 75
NdeI CATATG 3 cut(s) 9, 131, 323
NdeII GATC 1 cut(s) 523
NgoMIV GCCGGC 1 cut(s) 441
NlaIII CATG 2 cut(s) 79, 383
NlaIV GGNNCC 2 cut(s) 189, 294
NmeAIII GCCGAG 3 cut(s) 309, 393, 496
NmuCI GTSAC 1 cut(s) 467
NsiI ATGCAT 1 cut(s) 401
PceI AGGCCT 1 cut(s) 209
PcsI WCGNNNNNNNCGW 5 cut(s) 345, 399, 425, 431, 434
PdiI GCCGGC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 27, 500
PflFI GACNNNGTC 1 cut(s) 95
PleI GAGTC 1 cut(s) 94
PluTI GGCGCC 1 cut(s) 296
PmaCI CACGTG 1 cut(s) 408
PmlI CACGTG 1 cut(s) 408
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 448
Psp124BI GAGCTC 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 448
Psp6I CCWGG 3 cut(s) 105, 204, 450
PspCI CACGTG 1 cut(s) 408
PspGI CCWGG 3 cut(s) 105, 204, 450
PspN4I GGNNCC 2 cut(s) 189, 294
PspPI GGNCC 4 cut(s) 157, 229, 284, 448
PspPPI RGGWCCY 1 cut(s) 448
PstNI CAGNNNCTG 1 cut(s) 451
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 364
Sau3AI GATC 1 cut(s) 523
Sau96I GGNCC 4 cut(s) 157, 229, 284, 448
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 5 cut(s) 107, 185, 192, 206, 452
SduI GDGCHC 2 cut(s) 249, 364
SfaNI GCATC 2 cut(s) 386, 408
SfoI GGCGCC 1 cut(s) 294
SinI GGWCC 2 cut(s) 284, 448
SseBI AGGCCT 1 cut(s) 209
SspDI GGCGCC 1 cut(s) 292
SstI GAGCTC 1 cut(s) 364
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 5 cut(s) 105, 183, 190, 204, 450
StyI CCWWGG 2 cut(s) 75, 81
TaaI ACNGT 1 cut(s) 602
TaiI ACGT 2 cut(s) 393, 410
TaqI TCGA 1 cut(s) 402
TauI GCSGC 8 cut(s) 56, 64, 198, 293, 298, 370, 378, 480
TfiI GAWTC 2 cut(s) 27, 500
TscAI CASTG 1 cut(s) 377
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 6 cut(s) 56, 110, 162, 172, 218, 253
Tsp45I GTSAC 1 cut(s) 467
TspGWI ACGGA 2 cut(s) 294, 423
TspRI CASTG 1 cut(s) 377
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 284, 448
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.