pycom02g08840

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010997
Physical Location & Seq
Reverse (-)
6095604 .. 6096260
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08840.1

Sequence Viewer

Length: 657 bp
ATGCCTCGCTCTCAGAGATTTCTAGGCAGCAGCACCCCCGCCGCCAACTTCTCAACCGTATCGGCAGCTCCCCCCCCTGAGGCCGTGGCTCTGGAGTCGGATTTAGTTGTCATACTAGCAGCGCTGCTCTGCGCCGTCATATGCGTGGTGGGTCTTCTCGCCGTCGCCCGCTGCGCCTGGCTCCGCCGCGGCCCCGGCGGCAGAGCCGCTATCAGCTCGGCTTCTTCATCCGCCAACAAGGGCCTCAAGAAGAAGGTCCTCCAGTCCCTCCCCAAGTTCACGTACGGAACCGGCGGCGCCGAGCCGCCGCCAAAGCTGGGTTCGGAGTGCGCCATCTGCTTAGGGGAGTTCGTGGAGGGGCACGAGATCAGGATTCTGCCGCAGTGTGGGCACGTGTTCCACGTTGGATGCATCGACACGTGGCTTGGGTCTCACTCGTCGTGCCCGTCTTGTCGGCAGATCTTGGTGGTTGCTCGGTGTCACAAGTGCGGTGGGTTTCCGGCTCCAATATCTGAATCTGAGGCGGAGCTCAAGGCTCGCCAGGACCACACCAACCATTCAGCTCCTCCTCCGCCGGCGGCAGCGGCTGCTGCACCCACTACTGCCATTAGTATTAGTTCTTCTAATACGGCTCCAACTACCAATTTTCTCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

22.44

Weight (kDa)

8.69

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 109 - 152 5e-15 Ring finger domain
zf-RING_11 PF17123 109 - 138 7.9e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 109 - 151 1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 109 - 153 5e-06 zinc-RING finger domain
zf-C3HC4 PF00097 110 - 151 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 110 - 149 3.1e-06 RING-type zinc-finger
zf-rbx1 PF12678 126 - 152 2e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 189
AcvI CACGTG 2 cut(s) 394, 420
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 284
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 79, 317, 386
AflIII ACRYGT 2 cut(s) 393, 417
AjnI CCWGG 2 cut(s) 176, 540
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 5 cut(s) 68, 216, 316, 529, 563
AluI AGCT 5 cut(s) 68, 216, 316, 529, 563
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 1 cut(s) 435
AlwNI CAGNNNCTG 1 cut(s) 587
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 3 cut(s) 81, 190, 241
ApeKI GCWGC 9 cut(s) 27, 30, 65, 119, 124, 171, 581, 587, 590
AspLEI GCGC 5 cut(s) 124, 134, 176, 299, 332
AspS9I GGNCC 4 cut(s) 191, 241, 256, 544
AsuC2I CCSGG 1 cut(s) 195
AvaII GGWCC 2 cut(s) 256, 544
AxyI CCTNAGG 1 cut(s) 78
BaeGI GKGCMC 3 cut(s) 363, 393, 446
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 531
BarI GAAGNNNNNNTAC 2 cut(s) 604, 636
BauI CACGAG 1 cut(s) 362
BbrPI CACGTG 2 cut(s) 394, 420
BbsI GAAGAC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 9 cut(s) 39, 42, 77, 111, 131, 158, 574, 577, 593
BccI CCATC 1 cut(s) 341
BceAI ACGGC 4 cut(s) 68, 119, 146, 645
BciT130I CCWGG 2 cut(s) 178, 542
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 23, 116
BfoI RGCGCY 2 cut(s) 125, 300
BglI GCCNNNNNGGC 1 cut(s) 198
BglII AGATCT 1 cut(s) 459
Bme1390I CCNGG 3 cut(s) 178, 195, 542
Bme18I GGWCC 2 cut(s) 256, 544
BmgT120I GGNCC 4 cut(s) 191, 241, 256, 544
BmiI GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 633
BmrFI CCNGG 3 cut(s) 178, 195, 542
BmsI GCATC 2 cut(s) 398, 420
BpiI GAAGAC 1 cut(s) 146
BpmI CTGGAG 2 cut(s) 113, 245
Bpu10I CCTNAGC 1 cut(s) 340
BpuEI CTTGAG 2 cut(s) 230, 515
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 3 cut(s) 282, 394, 420
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 84, 187, 193
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 317, 386
Bse118I RCCGGY 2 cut(s) 290, 574
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 178, 542
BseDI CCNNGG 3 cut(s) 84, 187, 193
BseGI GGATG 2 cut(s) 227, 413
BseLI CCNNNNNNNGG 3 cut(s) 79, 317, 386
BseMII CTCAG 3 cut(s) 26, 69, 510
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 2 cut(s) 555, 558
BseSI GKGCMC 3 cut(s) 363, 393, 446
BseXI GCAGC 9 cut(s) 39, 42, 77, 111, 131, 158, 574, 577, 593
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 3 cut(s) 83, 192, 243
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 4 cut(s) 195, 291, 500, 575
BsiWI CGTACG 1 cut(s) 282
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 3 cut(s) 79, 317, 386
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 3 cut(s) 83, 192, 243
Bso31I GGTCTC 1 cut(s) 435
Bsp1286I GDGCHC 4 cut(s) 363, 393, 446, 531
Bsp143I GATC 2 cut(s) 366, 459
BspANI GGCC 3 cut(s) 83, 192, 243
BspCNI CTCAG 3 cut(s) 25, 70, 511
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 633
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 435
BsrFI RCCGGY 2 cut(s) 290, 574
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 2 cut(s) 290, 574
BssECI CCNNGG 3 cut(s) 84, 187, 193
BssMI GATC 2 cut(s) 366, 459
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 362
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 178, 542
Bst4CI ACNGT 1 cut(s) 58
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBAI YACGTR 3 cut(s) 282, 394, 420
BstC8I GCNNGC 3 cut(s) 169, 538, 576
BstDEI CTNAG 4 cut(s) 12, 78, 340, 519
BstDSI CCRYGG 2 cut(s) 84, 187
BstF5I GGATG 2 cut(s) 227, 413
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 2 cut(s) 125, 300
BstHHI GCGC 5 cut(s) 124, 134, 176, 299, 332
BstKTI GATC 2 cut(s) 369, 462
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 2 cut(s) 366, 459
BstMWI GCNNNNNNNGC 9 cut(s) 173, 195, 198, 313, 336, 388, 584, 587, 590
BstNI CCWGG 2 cut(s) 178, 542
BstSCI CCNGG 3 cut(s) 176, 193, 540
BstSLI GKGCMC 3 cut(s) 363, 393, 446
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 9 cut(s) 39, 42, 77, 111, 131, 158, 574, 577, 593
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 3 cut(s) 83, 192, 243
BtgI CCRYGG 2 cut(s) 84, 187
BtsCI GGATG 2 cut(s) 227, 413
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 3 cut(s) 169, 538, 576
CaiI CAGNNNCTG 1 cut(s) 587
CfoI GCGC 5 cut(s) 124, 134, 176, 299, 332
Cfr10I RCCGGY 2 cut(s) 290, 574
Cfr13I GGNCC 4 cut(s) 191, 241, 256, 544
Cfr42I CCGCGG 1 cut(s) 190
Csp6I GTAC 1 cut(s) 283
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 4 cut(s) 12, 78, 340, 519
DinI GGCGCC 1 cut(s) 298
DpnI GATC 2 cut(s) 368, 461
DpnII GATC 2 cut(s) 366, 459
EciI GGCGGA 4 cut(s) 173, 220, 539, 561
Ecl136II GAGCTC 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 256, 544
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 529
Eco72I CACGTG 2 cut(s) 394, 420
Eco81I CCTNAGG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 529
EcoO109I RGGNCCY 2 cut(s) 241, 256
EcoRII CCWGG 2 cut(s) 176, 540
EcoT22I ATGCAT 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaiI YATR 3 cut(s) 113, 140, 142
FaqI GGGAC 1 cut(s) 250
FauI CCCGC 2 cut(s) 46, 176
FauNDI CATATG 1 cut(s) 140
FokI GGATG 2 cut(s) 214, 420
FriOI GRGCYC 1 cut(s) 531
FspBI CTAG 2 cut(s) 23, 116
GlaI GCGC 5 cut(s) 123, 133, 175, 298, 331
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 2 cut(s) 113, 245
HaeII RGCGCY 2 cut(s) 125, 300
HaeIII GGCC 3 cut(s) 83, 192, 243
HapII CCGG 4 cut(s) 195, 291, 500, 575
HhaI GCGC 5 cut(s) 124, 134, 176, 299, 332
Hin1I GRCGYC 1 cut(s) 297
Hin6I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinP1I GCGC 5 cut(s) 122, 132, 174, 297, 330
HinfI GANTC 3 cut(s) 95, 373, 515
HpaII CCGG 4 cut(s) 195, 291, 500, 575
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 5 cut(s) 15, 100, 325, 514, 520
Hpy188III TCNNGA 3 cut(s) 92, 247, 370
Hpy8I GTNNAC 1 cut(s) 279
Hpy99I CGWCG 2 cut(s) 167, 442
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 4 cut(s) 281, 393, 402, 419
HpyCH4V TGCA 2 cut(s) 411, 593
HpyF10VI GCNNNNNNNGC 9 cut(s) 173, 195, 198, 313, 336, 388, 584, 587, 590
HpyF3I CTNAG 4 cut(s) 12, 78, 340, 519
HpySE526I ACGT 4 cut(s) 281, 393, 402, 419
Hsp92I GRCGYC 1 cut(s) 297
HspAI GCGC 5 cut(s) 122, 132, 174, 297, 330
KasI GGCGCC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 574
KroNI GCCGGC 1 cut(s) 576
KspI CCGCGG 1 cut(s) 190
Kzo9I GATC 2 cut(s) 366, 459
LmnI GCTCC 6 cut(s) 73, 186, 508, 526, 568, 637
Lsp1109I GCAGC 9 cut(s) 39, 42, 77, 111, 131, 158, 574, 577, 593
LweI GCATC 2 cut(s) 398, 420
MaeI CTAG 2 cut(s) 23, 116
MaeII ACGT 4 cut(s) 281, 393, 402, 419
MaeIII GTNAC 1 cut(s) 479
MalI GATC 2 cut(s) 368, 461
MboI GATC 2 cut(s) 366, 459
MboII GAAGA 4 cut(s) 146, 216, 262, 612
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 4 cut(s) 363, 393, 446, 531
MluCI AATT 1 cut(s) 643
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 78, 385
MnlI CCTC 9 cut(s) 15, 73, 254, 269, 278, 349, 514, 576, 579
Mph1103I ATGCAT 1 cut(s) 413
MreI CGCCGGCG 1 cut(s) 574
MroNI GCCGGC 1 cut(s) 574
MseI TTAA 1 cut(s) 655
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 3 cut(s) 171, 189, 584
MspI CCGG 4 cut(s) 195, 291, 500, 575
MspR9I CCNGG 3 cut(s) 178, 195, 542
MvaI CCWGG 2 cut(s) 178, 542
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 9 cut(s) 173, 195, 198, 313, 336, 388, 584, 587, 590
NaeI GCCGGC 1 cut(s) 576
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 195
NdeI CATATG 1 cut(s) 140
NdeII GATC 2 cut(s) 366, 459
NgoMIV GCCGGC 1 cut(s) 574
NlaIV GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 633
NmeAIII GCCGAG 2 cut(s) 197, 325
NmuCI GTSAC 1 cut(s) 479
NsiI ATGCAT 1 cut(s) 413
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 443
PdiI GCCGGC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 373, 515
Pfl23II CGTACG 1 cut(s) 282
PleI GAGTC 1 cut(s) 103
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 2 cut(s) 394, 420
PmlI CACGTG 2 cut(s) 394, 420
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 3 cut(s) 282, 394, 420
PpuMI RGGWCCY 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 531
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 176, 540
PspCI CACGTG 2 cut(s) 394, 420
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 2 cut(s) 176, 540
PspLI CGTACG 1 cut(s) 282
PspN4I GGNNCC 6 cut(s) 182, 193, 289, 298, 504, 633
PspPI GGNCC 4 cut(s) 191, 241, 256, 544
PspPPI RGGWCCY 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 531
SacII CCGCGG 1 cut(s) 190
SaqAI TTAA 1 cut(s) 655
Sau3AI GATC 2 cut(s) 366, 459
Sau96I GGNCC 4 cut(s) 191, 241, 256, 544
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 178, 195, 542
SduI GDGCHC 4 cut(s) 363, 393, 446, 531
SfaNI GCATC 2 cut(s) 398, 420
SfoI GGCGCC 1 cut(s) 298
Sfr303I CCGCGG 1 cut(s) 190
SgrAI CRCCGGYG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 190
SinI GGWCC 2 cut(s) 256, 544
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 245, 530
SmoI CTYRAG 2 cut(s) 245, 530
Sse9I AATT 1 cut(s) 643
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 2 cut(s) 23, 116
SstI GAGCTC 1 cut(s) 531
StyD4I CCNGG 3 cut(s) 176, 193, 540
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 4 cut(s) 284, 396, 405, 422
TaqI TCGA 1 cut(s) 414
TasI AATT 1 cut(s) 643
TfiI GAWTC 2 cut(s) 373, 515
Tru1I TTAA 1 cut(s) 655
Tru9I TTAA 1 cut(s) 655
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 9 cut(s) 27, 30, 65, 119, 124, 171, 581, 587, 590
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 256, 544
XspI CTAG 2 cut(s) 23, 116
Zsp2I ATGCAT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.