RLG00000016682

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010997
Physical Location & Seq
Reverse (-)
8917923 .. 8918579
657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016682

Sequence Viewer

Length: 657 bp
ATGCCTCGCTCCCAGAGATTTCTCACCACAGCAAACTCAACGATGAAGTACGCCGCCGCGCCGCCTCCTGAGGCCGTGACCTTGGAATCCGACTTTGTTGTTATCCTGGCGGCGCTTTTATGCGCCGTCATATGCGTCGTGGGGCTGCTCGCCGTGGCGCGCTGCGCCTGGCTGCGACGCGCGCCAGGCGGGGGCCGGGCCGGTCAACCCGGCTCCGCACAGGCCTCTTCTGCCAACAAAGGGCTGAAAAAGAAGGTCCTGCAGCAGCTCCCCAAGTTCATCTACGGCGACGGAGGACCGTCGGAGCCGCCGCCGAAGATGGGGTCGGAGTGCGCCATATGCCTCGGCGAGTTCGCGGAGGGAGATGAGATTAGGGTTCTGCCGCAGTGCGGGCACGTTTTCCACGTCGGATGCATCGACACGTGGCTAGGGTCCCACTCGTCTTGTCCGTCGTGTCGGCAGATCCTGGTGGTGGCGCGGTGTCACAAGTGCGGCAACTTCCCCGCCATTTCTGACGCTGAGCTCAAAATTCGGCAAGATCATATTTACAATTCTGCCCCTCCGCCACTCGCCTTGGCCGCCGCCGCCGCCACAACTACTAGTTGTAGTAGCAGCAGCAGTAATAGTAATAGTAAGAGCAATATTTTCTTACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

22.82

Weight (kDa)

8.75

Isoelectric Point (pI)

65.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 110 - 153 5.9e-16 Ring finger domain
zf-RING_11 PF17123 110 - 139 1.1e-12 RING-like zinc finger
zf-C3HC4_2 PF13923 110 - 152 3.2e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 110 - 154 8.8e-07 zinc-RING finger domain
zf-C3HC4 PF00097 111 - 152 5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 111 - 150 1e-06 RING-type zinc-finger
zf-rbx1 PF12678 119 - 153 2.3e-07 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 121 - 159 3.4e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 528
AcvI CACGTG 1 cut(s) 423
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 5 cut(s) 191, 240, 320, 389, 472
AflIII ACRYGT 1 cut(s) 420
AhlI ACTAGT 1 cut(s) 599
AjiI CACGTC 1 cut(s) 406
AjnI CCWGG 4 cut(s) 105, 167, 184, 465
AluBI AGCT 2 cut(s) 268, 523
AluI AGCT 2 cut(s) 268, 523
Alw21I GWGCWC 1 cut(s) 525
AlwI GGATC 1 cut(s) 457
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 5 cut(s) 72, 193, 198, 222, 576
ApeKI GCWGC 7 cut(s) 145, 162, 172, 262, 265, 612, 615
ApoI RAATTY 1 cut(s) 528
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspS9I GGNCC 5 cut(s) 193, 198, 256, 296, 432
AsuC2I CCSGG 2 cut(s) 197, 210
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 3 cut(s) 256, 296, 432
AxyI CCTNAGG 1 cut(s) 69
BaeGI GKGCMC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 525
BbrPI CACGTG 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 7 cut(s) 132, 149, 159, 274, 277, 624, 627
BccI CCATC 1 cut(s) 313
BceAI ACGGC 4 cut(s) 59, 110, 137, 301
BciT130I CCWGG 4 cut(s) 107, 169, 186, 467
BcnI CCSGG 2 cut(s) 197, 210
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 2 cut(s) 428, 600
BfmI CTRYAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 116
BlpI GCTNAGC 1 cut(s) 519
Bme1390I CCNGG 6 cut(s) 107, 169, 186, 197, 210, 467
Bme18I GGWCC 3 cut(s) 256, 296, 432
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 5 cut(s) 193, 198, 256, 296, 432
BmiI GGNNCC 5 cut(s) 194, 214, 306, 433, 434
BmrFI CCNGG 6 cut(s) 107, 169, 186, 197, 210, 467
BmsI GCATC 2 cut(s) 401, 423
Bpu1102I GCTNAGC 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 197, 210
BsaAI YACGTR 1 cut(s) 423
BsaJI CCNNGG 4 cut(s) 81, 153, 343, 573
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 240, 320, 389, 472
Bse118I RCCGGY 1 cut(s) 200
Bse21I CCTNAGG 1 cut(s) 69
BseBI CCWGG 4 cut(s) 107, 169, 186, 467
BseDI CCNNGG 4 cut(s) 81, 153, 343, 573
BseGI GGATG 1 cut(s) 416
BseLI CCNNNNNNNGG 5 cut(s) 191, 240, 320, 389, 472
BseMII CTCAG 2 cut(s) 60, 510
BsePI GCGCGC 2 cut(s) 158, 180
BseSI GKGCMC 1 cut(s) 396
BseXI GCAGC 7 cut(s) 132, 149, 159, 274, 277, 624, 627
Bsh1236I CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
BshFI GGCC 5 cut(s) 74, 195, 200, 224, 578
BsiHKAI GWGCWC 1 cut(s) 525
BsiSI CCGG 3 cut(s) 196, 201, 210
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 5 cut(s) 191, 240, 320, 389, 472
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 5 cut(s) 74, 195, 200, 224, 578
Bsp1286I GDGCHC 2 cut(s) 396, 525
Bsp143I GATC 2 cut(s) 462, 538
Bsp1720I GCTNAGC 1 cut(s) 519
BspANI GGCC 5 cut(s) 74, 195, 200, 224, 578
BspCNI CTCAG 2 cut(s) 61, 511
BspFNI CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
BspLI GGNNCC 5 cut(s) 194, 214, 306, 433, 434
BspMAI CTGCAG 1 cut(s) 264
BspPI GGATC 1 cut(s) 457
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 4 cut(s) 81, 153, 343, 573
BssHII GCGCGC 2 cut(s) 158, 180
BssMI GATC 2 cut(s) 462, 538
BssT1I CCWWGG 2 cut(s) 81, 573
Bst2UI CCWGG 4 cut(s) 107, 169, 186, 467
Bst4CI ACNGT 1 cut(s) 300
Bst6I CTCTTC 1 cut(s) 232
BstBAI YACGTR 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 150, 160, 182, 392
BstDEI CTNAG 2 cut(s) 69, 519
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
BstH2I RGCGCY 1 cut(s) 116
BstKTI GATC 2 cut(s) 465, 541
BstMBI GATC 2 cut(s) 462, 538
BstMWI GCNNNNNNNGC 9 cut(s) 164, 181, 186, 230, 339, 391, 578, 584, 587
BstNI CCWGG 4 cut(s) 107, 169, 186, 467
BstSCI CCNGG 6 cut(s) 105, 167, 184, 195, 208, 465
BstSFI CTRYAG 1 cut(s) 260
BstSLI GKGCMC 1 cut(s) 396
BstUI CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
BstV1I GCAGC 7 cut(s) 132, 149, 159, 274, 277, 624, 627
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 69
BsuRI GGCC 5 cut(s) 74, 195, 200, 224, 578
BtgI CCRYGG 1 cut(s) 153
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 1 cut(s) 416
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 1 cut(s) 392
Cac8I GCNNGC 4 cut(s) 150, 160, 182, 392
CaiI CAGNNNCTG 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 193, 198, 256, 296, 432
CseI GACGC 3 cut(s) 124, 186, 524
Csp6I GTAC 1 cut(s) 49
CspCI CAANNNNNGTGG 4 cut(s) 425, 460, 555, 590
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 69, 519
DpnI GATC 2 cut(s) 464, 540
DpnII GATC 2 cut(s) 462, 538
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
EciI GGCGGA 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 81, 573
Eco147I AGGCCT 1 cut(s) 224
Eco24I GRGCYC 1 cut(s) 525
Eco47I GGWCC 3 cut(s) 256, 296, 432
Eco53kI GAGCTC 1 cut(s) 523
Eco72I CACGTG 1 cut(s) 423
Eco81I CCTNAGG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 2 cut(s) 256, 432
EcoRII CCWGG 4 cut(s) 105, 167, 184, 465
EcoT14I CCWWGG 2 cut(s) 81, 573
EcoT22I ATGCAT 1 cut(s) 416
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 2 cut(s) 81, 573
FaiI YATR 6 cut(s) 121, 131, 133, 338, 340, 543
FaqI GGGAC 1 cut(s) 418
FauI CCCGC 3 cut(s) 182, 383, 511
FauNDI CATATG 2 cut(s) 131, 338
FokI GGATG 1 cut(s) 423
FriOI GRGCYC 1 cut(s) 525
FspBI CTAG 2 cut(s) 428, 600
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 5 cut(s) 74, 195, 200, 224, 578
HapII CCGG 3 cut(s) 196, 201, 210
HgaI GACGC 3 cut(s) 124, 186, 524
HincII GTYRAC 1 cut(s) 206
HindII GTYRAC 1 cut(s) 206
HinfI GANTC 1 cut(s) 86
HpaII CCGG 3 cut(s) 196, 201, 210
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 206
Hpy188I TCNGA 5 cut(s) 91, 304, 328, 410, 514
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 1 cut(s) 206
Hpy99I CGWCG 6 cut(s) 140, 180, 293, 304, 410, 454
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4IV ACGT 3 cut(s) 396, 405, 422
HpyCH4V TGCA 2 cut(s) 262, 414
HpyF10VI GCNNNNNNNGC 9 cut(s) 164, 181, 186, 230, 339, 391, 578, 584, 587
HpyF3I CTNAG 2 cut(s) 69, 519
HpySE526I ACGT 3 cut(s) 396, 405, 422
KflI GGGWCCC 1 cut(s) 432
Kzo9I GATC 2 cut(s) 462, 538
LmnI GCTCC 4 cut(s) 14, 218, 273, 304
Lsp1109I GCAGC 7 cut(s) 132, 149, 159, 274, 277, 624, 627
LweI GCATC 2 cut(s) 401, 423
MaeI CTAG 2 cut(s) 428, 600
MaeII ACGT 3 cut(s) 396, 405, 422
MaeIII GTNAC 2 cut(s) 76, 482
MalI GATC 2 cut(s) 464, 540
MboI GATC 2 cut(s) 462, 538
MboII GAAGA 2 cut(s) 219, 328
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 396, 525
MluCI AATT 2 cut(s) 528, 550
MmeI TCCRAC 4 cut(s) 114, 282, 306, 388
MnlI CCTC 8 cut(s) 15, 64, 75, 235, 287, 352, 353, 570
Mph1103I ATGCAT 1 cut(s) 416
MspI CCGG 3 cut(s) 196, 201, 210
MspR9I CCNGG 6 cut(s) 107, 169, 186, 197, 210, 467
MteI GCGCNGCGC 1 cut(s) 163
MvaI CCWGG 4 cut(s) 107, 169, 186, 467
MvnI CGCG 6 cut(s) 59, 160, 180, 182, 356, 478
MwoI GCNNNNNNNGC 9 cut(s) 164, 181, 186, 230, 339, 391, 578, 584, 587
NciI CCSGG 2 cut(s) 197, 210
NdeI CATATG 2 cut(s) 131, 338
NdeII GATC 2 cut(s) 462, 538
NlaIV GGNNCC 5 cut(s) 194, 214, 306, 433, 434
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 2 cut(s) 76, 482
NsiI ATGCAT 1 cut(s) 416
PauI GCGCGC 2 cut(s) 158, 180
PceI AGGCCT 1 cut(s) 224
PcsI WCGNNNNNNNCGW 3 cut(s) 402, 414, 446
PfeI GAWTC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 423
PmlI CACGTG 1 cut(s) 423
Ppu21I YACGTR 1 cut(s) 423
PpuMI RGGWCCY 2 cut(s) 256, 432
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 2 cut(s) 256, 432
Psp6I CCWGG 4 cut(s) 105, 167, 184, 465
PspCI CACGTG 1 cut(s) 423
PspGI CCWGG 4 cut(s) 105, 167, 184, 465
PspN4I GGNNCC 5 cut(s) 194, 214, 306, 433, 434
PspPI GGNCC 5 cut(s) 193, 198, 256, 296, 432
PspPPI RGGWCCY 2 cut(s) 256, 432
PstI CTGCAG 1 cut(s) 264
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 462
PteI GCGCGC 2 cut(s) 158, 180
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 1 cut(s) 525
Sau3AI GATC 2 cut(s) 462, 538
Sau96I GGNCC 5 cut(s) 193, 198, 256, 296, 432
ScrFI CCNGG 6 cut(s) 107, 169, 186, 197, 210, 467
SduI GDGCHC 2 cut(s) 396, 525
SetI ASST 7 cut(s) 83, 258, 270, 399, 408, 425, 525
SfaNI GCATC 2 cut(s) 401, 423
SfcI CTRYAG 1 cut(s) 260
SinI GGWCC 3 cut(s) 256, 296, 432
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 2 cut(s) 528, 550
SseBI AGGCCT 1 cut(s) 224
SspI AATATT 1 cut(s) 643
SspMI CTAG 2 cut(s) 428, 600
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 224
StyD4I CCNGG 6 cut(s) 105, 167, 184, 195, 208, 465
StyI CCWWGG 2 cut(s) 81, 573
TaaI ACNGT 1 cut(s) 300
TaiI ACGT 3 cut(s) 399, 408, 425
TaqI TCGA 1 cut(s) 417
TasI AATT 2 cut(s) 528, 550
TfiI GAWTC 1 cut(s) 86
TscAI CASTG 1 cut(s) 392
TseFI GTSAC 2 cut(s) 76, 482
TseI GCWGC 7 cut(s) 145, 162, 172, 262, 265, 612, 615
Tsp45I GTSAC 2 cut(s) 76, 482
TspDTI ATGAA 2 cut(s) 59, 268
TspGWI ACGGA 2 cut(s) 306, 438
TspRI CASTG 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 256, 296, 432
XapI RAATTY 1 cut(s) 528
XspI CTAG 2 cut(s) 428, 600
Zsp2I ATGCAT 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.